| targeting and degradation of p53 by e6 of human papillomavirus type 16 is preferential for the 1620+ p53 conformation. | e6-mediated degradation of p53 is believed to play a role in the transformation of cells by high-risk types of human papillomavirus. in order to explore the structural requirements for targeting of p53 we have compared e6-mediated degradation of variant p53 forms expressed in vitro. complete degradation was observed in samples containing monomers, dimers and higher molecular weight structures of wild-type p53, indicating that e6 targets all quaternary forms of wild-type p53. wild-type human and ... | 1993 | 7690928 |
| tata-binding protein and the retinoblastoma gene product bind to overlapping epitopes on c-myc and adenovirus e1a protein. | using a protein binding assay, we show that the amino-terminal 204 amino acids of the c-myc protein interact directly with a key component of the basal transcription factor tfiid, the tata box-binding protein (tbp). essentially the same region of the c-myc protein also binds the product of the retinoblastoma gene, the rb protein. c-myc protein coimmunoprecipitates with tbp in lysates of mammalian cells, demonstrating that the proteins are also complexed in vivo. a short peptide that spans the rb ... | 1993 | 7690963 |
| hypothetical structure of the membrane-associated e5 oncoprotein of human papillomavirus type 16. | | 1994 | 7698457 |
| involvement of prb family in tgf beta-dependent epithelial cell hypertrophy. | although renal hypertrophy is often associated with the progressive loss of renal function, the mechanism of hypertrophy is poorly understood. in both primary cultures of rabbit proximal tubules and nrk-52e cells (a renal epithelial cell line), transforming growth factor beta 1 (tgf beta) converted epidermal growth factor (egf)-induced hyperplasia into hypertrophy. tgf beta did not affect egf-induced increases in c-fos mrna abundance or cyclin e protein abundance, but inhibited egf-induced entry ... | 1995 | 7698989 |
| involvement of the p53 tumor suppressor in repair of u.v.-type dna damage. | the tumor suppressor p53 plays a central role in the cellular responses to genotoxic stress. besides its well known role in activation of the g1 checkpoint after exposure to agents like ionizing radiation and its role in apoptosis, the possibility exists that p53 may have additional roles, such as in dna repair. for example, p53, is known to bind to single strand dna such as would occur during repair events, and the proteins encoded by two p53-regulated genes have previously been found to bind t ... | 1995 | 7700629 |
| intralesional interferon alpha-2b therapy for buschke-loewenstein tumour. | a patient with a buschke-loewenstein tumour associated with human papillomavirus type 6/11 is reported. he was intralesionally treated with human recombinant alpha-2b interferon (9 x 10(6) iu/day) three times weekly. the tumour completely disappeared within 5 months of continuous treatment which was well tolerated. sixteen months after completion of therapy the patient remains well and free of disease. | 1994 | 7701880 |
| [genital herpes simplex virus infection among men screened for genital papillomavirus]. | our aim was to assess the frequency of herpetic genital infection (hsv) among men attending a human papillomavirus (hpv) screening centre. clinical screening of a herpetic lesion was completed with biological detection of hsv by cell culture and by polymerase chain reaction (pcr). we also evaluated the role of the male viral factor on the female partners. | 1994 | 7702261 |
| inactivation of the p53 gene by either mutation or hpv infection is extremely frequent in human oral squamous cell carcinoma cell lines. | the state of p53 tumour suppressor and the frequency of high-risk human papillomavirus (hpv) infections were studied in nine human oral cancer cell lines. three cancer cell lines (scc-4, tu-177 and fadu) had similar amounts of p53 transcripts to normal cells, but contained significantly higher levels of p53 protein than the normal control cells. sequencing highly conserved open reading frames of the p53 gene of these cancer cells showed point mutations in the scc-4 and tu-177 cell lines, a base ... | 1994 | 7703804 |
| establishment and characterization of a cell line (cumc-3) derived from a human squamous carcinoma of the uterine cervix. | a new cell line, cumc-3, has been derived from an invasive nonkeratinizing squamous cell carcinoma of the uterine cervix in a 32-year-old patient. it has been maintained in long-term culture for 59 months, and passaged over 310 times. monolayer-cultured cells were polygonal in shape, showing a pavement-like arrangement and a tendency to pile up without contact inhibition. the epithelial nature of the cultured cumc-3 cells was also confirmed by transmission electron microscopy which demonstrated ... | 1995 | 7705700 |
| human t-cell lymphotropic virus type i and severe neoplasia of the cervix in jamaica. | human t-cell lymphotropic virus type i (htlv-i) was associated with carcinoma of the cervix in japan in a recent study that compared hospital cases with healthy population-based controls. to test this relationship in women more alike for cervical neoplasia risk factors (including sexual behavior and human papilloma virus; hpv), we enrolled consecutive patients from a colposcopy clinic in kingston, jamaica (an htlv-i endemic area). patients underwent pap smear, colposcopy, biopsy and cervical swa ... | 1995 | 7705929 |
| human papillomavirus infection is transient in young women: a population-based cohort study. | the prevalence of human papillomavirus (hpv) infection in cervical cell scrapes from a cohort of 276 young women was determined by a general two-step polymerase chain reaction. hpv infection fluctuated among young women during a 2-year interval. the total prevalence of hpv infection decreased from 21% to 8.3%. the most prevalent hpv types at enrollment were hpv-16 (3.3%) and hpv-6 (2.9%). at follow-up, the most common type was hpv-16 (2.9%), while no hpv-6 was detected. in 2 women only, the same ... | 1995 | 7706782 |
| the rev protein of human immunodeficiency virus type 1 counteracts the effect of an au-rich negative element in the human papillomavirus type 1 late 3' untranslated region. | we have identified a sequence in the late 3' untranslated region of human papillomavirus type 1 mrnas that acts posttranscriptionally to repress gene expression. deletion analysis localized the inhibitory element to an au-rich sequence between nucleotides 6958 and 6984 on the human papillomavirus type 1 genome. this sequence inhibits gene expression in an orientation-dependent manner. upon transfection of eucaryotic cells with plasmids containing this sequence, approximately 4-fold-lower cytopla ... | 1995 | 7707519 |
| human papillomavirus not found in squamous and large cell lung carcinomas by polymerase chain reaction. | | 1995 | 7712451 |
| [detection of cervical human papillomavirus infection from women with external genital condyloma acuminata]. | cervical biopsy specimens were taken from 36 women with external genital condyloma acuminata in order to diagnose a concomitant cervical human papillomavirus (hpv) infection. positive hpv infection rate of cervix was 16% (6/36) by routine visual examination, 69% (25/36) by colposcopy and 56% (18/32) by pathology. among those patients 31% (10/32) have grade i-ii cervical intraepithelial neoplasia (cin) according to pathology. in 8 cases, both vulva and cervical specimens were examined by southern ... | 1994 | 7712891 |
| sperm as a noninvasive gene delivery system for preimplantation embryos. | to determine if sperm could be manipulated to be a noninvasive transport carrier for the delivery of gene fragments to the blastocyst. | 1995 | 7720929 |
| human papillomavirus infection in human immunodeficiency virus-seropositive women. | to compare the prevalence of human papillomavirus (hpv) infections in women who are seropositive and seronegative for human immunodeficiency virus (hiv), and to determine if associations between hpv and cervical disease are altered in hiv-seropositive women. | 1995 | 7724095 |
| prognostic significance of human papillomavirus dna in vulvar carcinoma. | to determine the histopathologic, epidemiologic, and prognostic significance of human papillomavirus (hpv) dna in primary invasive vulvar cancer. | 1995 | 7724101 |
| reconstitution of p53-ubiquitinylation reactions from purified components: the role of human ubiquitin-conjugating enzyme ubc4 and e6-associated protein (e6ap). | the e6 protein of the high-risk human papillomaviruses inactivates the tumor suppressor protein p53 by stimulating its ubiquitinylation and subsequent degradation. ubiquitinylation is a multistep process involving a ubiquitin-activating enzyme, one of many distinct ubiquitin-conjugating enzymes, and in certain cases, a ubiquitin ligase. in human papillomavirus-infected cells, e6 and the e6-associated protein are thought to act as a ubiquitin-protein ligase in the ubiquitinylation of p53. here we ... | 1995 | 7724550 |
| an evaluation of human papillomavirus testing for intermediate- and high-risk types as triage before colposcopy. | our purpose was to evaluate the role of testing for intermediate- and high-risk human papillomavirus by use of a hybrid capture technique for predicting which patients with abnormal papanicolaou smears are most likely to have squamous intraepithelial lesions or cancer. | 1995 | 7726250 |
| human papillomavirus and esophageal squamous cell carcinoma. | | 1995 | 7729660 |
| human papillomavirus and cervical intraepithelial neoplasia grade ii-iii: a population-based case-control study. | the association between certain human papillomaviruses (hpv) and cervical intraepithelial neoplasia (cin) is well documented, but there is uncertainty about the strength of association and the role of co-factors is unclear. this population-based case-control study in norwegian women 20-44 years of age included 103 cases with histologically confirmed cin ii-iii and 234 age-matched and randomly selected controls. cytological specimens from the cervix were analyzed using the polymerase chain reacti ... | 1995 | 7729940 |
| oral and laryngeal papilloma: a pediatric manifestation of sexually transmitted disease? | laryngeal and oral papilloma in pediatric patients are generally considered transmitted by maternal fetal transmission. in adults and immunocompromised patients, it is well documented that human papilloma virus (hpv) of the oropharynx is sexually transmitted. the possibility of sexual transmission of oral and laryngeal papilloma in children by oral genital contact is discussed. | 1995 | 7729997 |
| the human papillomavirus (hpv)-6 and hpv-16 e5 proteins co-operate with hpv-16 e7 in the transformation of primary rodent cells. | e5 is the smallest transforming protein encoded by the human papillomaviruses (hpvs). it has been shown to promote anchorage-independent growth in established nih 3t3 cells, an activity that is enhanced in the presence of epidermal growth factor (egf). it is thought that this activity of e5 is brought about by an increase in the half-life of stimulated egf receptors, possibly through the perturbation of receptor processing. recent studies have also shown that e5 can co-operate with hpv-16 e7 to ... | 1995 | 7730808 |
| serum antibody against unfused recombinant e7 protein of human papillomavirus type 16 in cervical cancer patients. | sera were examined for the presence of antibody against e7 protein of human papillomavirus type 16 (hpv-16) by western blot analysis using the bacterially derived unfused protein. the occurrence rates of anti-e7 antibody against hpv-16 were 14.1% (10/71) in cervical cancer patients, 0% (0/48) in cervical intraepithelial neoplasia patients, and 0% (0/41) in female non-malignant patients. three patients (one with endometrial cancer, one with breast cancer, and one male patient with colon polyp) ou ... | 1995 | 7737906 |
| pathology and management of vulval pain and pruritus. | vulval pain and pruritus are symptoms associated with benign or malignant diseases. the paucity of information regarding aetiological factors confounds a logical approach to successful therapeutic and preventative strategies. research continues to address the possible relationship between human papillomavirus and benign, premalignant and malignant vulval conditions. although present in many conditions, oncogenesis caused by human papillomavirus has yet to be proven as a cause of vulvar premalign ... | 1995 | 7742509 |
| binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells. | infection of cells by human papillomaviruses (hpvs) associated with malignant genital lesions has not been studied because of the lack of an in vitro system and the unavailability of virions. we have now used virus-like particles (vlps) of hpv type 33 to analyze the initial events in the interaction of the hpv capsid with cell lines. binding of vlps to hela cells was observed in biochemical assays and by immunofluorescence. vlp binding was inhibited by antisera raised against vlps but not by mon ... | 1995 | 7745672 |
| human papillomavirus type 31b late gene expression is regulated through protein kinase c-mediated changes in rna processing. | expression of the human papillomavirus (hpv) capsid genes, l1 and l2, as well as amplification of viral dna and virion assembly occur in the terminally differentiated layers of infected stratified squamous epithelium in vivo. these processes can be duplicated in the laboratory through the use of organotypic or raft cultures. when cin612 cells, which contain episomal copies of the high-risk hpv type 31b, are allowed to differentiate in raft cultures, the expression of transcripts encoding the ear ... | 1995 | 7745684 |
| oligomerisation of full length p53 contributes to the interaction with mdm2 but not hpv e6. | the tumour suppressor protein p53 normally functions as a tetramer in a defined conformational state. mutations within p53 which contribute to cancer development frequently induce a conformational shift in the protein which correlates with loss of wild type growth suppressor functions. both the cell encoded mdm2 protein and the human papillomavirus oncoprotein e6 can regulate p53 function and we have examined the interaction of these proteins with p53. the e6/p53 association is sensitive to conf ... | 1995 | 7753547 |
| differentiated carcinomas develop as a consequence of the thyroid specific expression of a thyroglobulin-human papillomavirus type 16 e7 transgene. | the oncogenic properties of the high risk human papillomaviruses (hpv) e7 protein are attributed to its interaction with the retinoblastoma susceptibility gene product rb1 and other related proteins. we report here the generation of a transgenic model expressing the e7 oncogene of hpv16 in thyroid follicular cells, under control of the bovine thyroglobulin gene promoter. transgenics develop differentiated and functionally regulated thyroid goitres, due to thyroid cell proliferation and accumulat ... | 1995 | 7753555 |
| naked-eye inspection of the cervix after acetic acid application may improve the predictive value of negative cytologic screening. | the purpose of this study was to assess the predictive value of naked-eye inspection of the cervix (nic) after acetic acid application as an adjunct to papanicolaou (pap) testing for cervical cancer screening. | 1994 | 7755697 |
| detection of human papillomavirus dna on surgeons' gloves: possible implications for patients with cervical cancer. | | 1995 | 7759166 |
| invasive squamous-cell carcinoma in giant anorectal condyloma (buschke-löwenstein tumor). | giant condyloma acuminata, first described by buschke and löwenstein in 1925 as a penile lesion, is extremely rare in the anorectal region. the cauliflower-like tumor behaves clinically in a malignant fashion, although it shows no histomorphological criteria of malignancy. up to the time of writing only 33 cases of anorectal origin, 42% with malignant transformation, have been published. the authors report 2 more cases of squamous-cell carcinoma in giant anorectal condylomata acuminata. buschke- ... | 1995 | 7760649 |
| sequence determination of human papillomavirus type 6a and assembly of virus-like particles in saccharomyces cerevisiae. | human papillomavirus 6a (hpv6a), the most abundant hpv6 subtype, was detected in a vulvar condyloma acuminatum. the complete genome of hpv6a was cloned, and its dna sequence was shown to be over 97% identical to the hpv6b sequence. of the eight open reading frames (orfs) of hpv6a, only the imputed amino acid sequence of the major capsid protein l1 was identical to the corresponding hpv6b sequence; all other hpv6a orfs showed amino acid changes compared to the hpv6b orfs. the hpv6a l1 or the l1 + ... | 1995 | 7778283 |
| human papillomavirus and widespread cutaneous carcinoma after puva photochemotherapy. | oral psoralen with uv-a (puva) photochemotherapy is known to cause cutaneous malignancies and has been associated with cutaneous immunosuppression. human papillomavirus infection has also been associated with cutaneous malignancies and with immunosuppressed individuals. we therefore sought evidence of human papillomavirus infection in a patient with a long history of puva therapy and multiple cutaneous malignancies. | 1995 | 7778923 |
| overexpression of bcl-xs sensitizes mcf-7 cells to chemotherapy-induced apoptosis. | resistance to apoptosis plays an important role in tumors that are refractory to chemotherapy. we report that bcl-xl, which functions like bcl-2 to inhibit apoptosis, is highly expressed in mcf-7 human breast carcinoma cells. we used bcl-xs, a dominant negative inhibitor of bcl-2 and bcl-xl, to demonstrate the role of these genes in modulating chemotherapy-induced apoptosis. bcl-xs overexpressed in mcf-7 cells by stable transfection does not affect viability by itself but induces a marked increa ... | 1995 | 7780958 |
| combined oral carcinogenicity of hpv-16 and benzo(a)pyrene: an in vitro multistep carcinogenesis model. | we previously immortalized normal human oral keratinocytes by transfection with recombinant hpv-16 dna and subsequently exposed the cells to benzo(a)pyrene for 7 days. the exposure to benzo(a)pyrene modified the immortalized cells: the modified cells (hok-16b-bap) proliferated in an ordinary culture medium containing physiological calcium level (1.5 mm), but demonstrated only enhanced proliferation capacity without tumor formation in nude mice and failed to show in vitro anchorage-independency. ... | 1995 | 7784058 |
| expression of cytochrome p450 and microsomal epoxide hydrolase in cervical and oral epithelial cells immortalized by human papillomavirus type 16 e6/e7 genes. | epidemiological evidence suggests that the presence of human papillomaviruses (hpv), when combined with smoking behaviors, considerably enhances the risk of developing oral, cervical, vulvar, and/or anal carcinomas. it is well established that the cytochrome p450 (cyp), microsomal epoxide hydrolase (meh), and other biotransformation enzymes are important modulators of the bioactivation and detoxification of many environmental chemicals, including constituents of tobacco smoke such as certain nit ... | 1995 | 7788860 |
| establishment and characterization of a human cell line from a squamous carcinoma of the tongue. | a human squamous carcinoma cell line, pwh-s1, has been established from the metastatic lymph node of a chinese patient with a squamous cell carcinoma of the tongue. the fibroblast-free culture has been propagated in dmem supplemented with 10% fetal bovine serum for more than 100 passages. pwh-s1 cells showed anchorage-independent growth in 0.3% agar solution. pwh-s1 cell line exhibited a monolayer growth and loss of contact inhibition. the in vitro doubling time of the pwh-s1 cell line was appro ... | 1995 | 7788927 |
| drug therapies for sexually transmitted diseases. clinical and economic considerations. | sexually transmitted diseases (stds) are common, and result in immense social and economic costs. in some countries they have a major demographic impact. because many stds facilitate the transmission of hiv, the consequences of stds are further increasing. at the same time, this association between stds and hiv provides one of the ways in which drug therapy should be very cost effective. the perspective taken in this article is a societal one, and broader issues than those directly related to dr ... | 1995 | 7789285 |
| interferons alpha, beta and gamma therapy of anogenital human papillomavirus infections. | anogenital condyloma acuminatum (genital warts) is the most commonly diagnosed sexually transmitted viral disease in the united states. at least 14 of the more than 60 types of human papillomaviruses (hpvs) are responsible for condyloma acuminatum. anogenital condyloma acuminatum has a broad spectrum of manifestations in men and women, including subclinical latent infection, clinically apparent warts, abnormal genital cytology and squamous carcinoma. traditional therapeutic modalities include cy ... | 1995 | 7792318 |
| test reliability is critically important to molecular epidemiology: an example from studies of human papillomavirus infection and cervical neoplasia. | to demonstrate that it is critically important to achieve excellent test reliability before conducting full-scale molecular epidemiological studies, data were compared from two consecutive case-control studies of human papillomavirus (hpv) infection and cervical intraepithelial neoplasia. the major methodological difference between the two studies was the much greater reliability of the hpv test used in the second study. although the first study used an assay considered state-of-the-art at that ... | 1994 | 7794294 |
| kinetic and equilibrium binding studies of the human papillomavirus type-16 transcription regulatory protein e2 interacting with core enhancer elements. | the human papillomaviruses (hpvs) are a family of dna viruses which cause benign tumours of the skin and mucosa that infrequently progress to malignant carcinoma. the e2 open reading frame of hpv is thought to encode a papillomavirus-specific transcription factor which also has a role in viral replication. the e2 proteins of all papillomaviruses studied to date have been shown to bind specifically to the common conserved sequence acc(n)6ggt found at multiple locations in their genomes. in the ca ... | 1994 | 7800477 |
| detection of human papillomavirus dna in colorectal adenomas. | to assess the presence of different types of human papillomavirus (hpv) dna in colorectal adenomas. | 1995 | 7802580 |
| sexually transmitted diseases from the tropics. an overview. | the tropical ecosystem as related to sexually transmitted diseases and their epidemiology is outlined in this article. the predominance of first-generation bacterial stds with more florid presentations, together with a back-ground of more covert infections, such as with chlamydia and human papillomavirus, are noted. presentations of infections in people returning from the tropics are highlighted, and particular features of the various infections relevant to their tropical origin are discussed. t ... | 1994 | 7805303 |
| human papillomavirus in condyloma acuminata and other benign lesions of the female genital tract. | pcr was used to detect (hpv) in condyloma acuminata (ca), pseudocondyloma whose cause is unclear, and the other benign lesions in female genital tract. the results showed that hpv prevalence rate in ca was 98.2%, type 6 was in 11 was 85.7%, and that in pseudocondyloma, cervical polyp, cervical erosion and chronic vaginitis was 40.4%, 76.2%, 77.3% and 16.0%, respectively. even in normal vaginal tissue, there existed hpv subclinical infection. hpv prevalence rate in swabs from patient's underwears ... | 1994 | 7805465 |
| cell-mediated immunity in invasive cervical carcinoma associated with human papillomavirus types 16 and 18 infection. | | 1994 | 7805989 |
| management of the female hiv-infected patient. | management of women with hiv infection or aids should follow the established guidelines for antiretroviral therapy and prevention and treatment of opportunistic complications of hiv infection. gynecological manifestations of hiv are primarily cervical dysplasia and cancer associated with human papillomavirus (hpv) infection and vaginal and mucocutaneous candidiasis. human papillomavirus-associated cervical dysplasia/neoplasia is more common in women with advanced rather than early hiv disease, a ... | 1994 | 7811546 |
| association of human leukocyte antigen-b1*03 with cervical cancer in japanese women aged 35 years and younger. | an association of human leukocyte antigen (hla)-dqw3 alleles with squamous cell carcinoma (scc) of the cervix has been reported in some european populations, but the significance of hla-dqw3 has not been examined in other populations to the authors' knowledge. the interaction between hla-dqw3 and human papillomavirus (hpv) in scc remains to be clarified. | 1995 | 7812922 |
| cis-acting components of human papillomavirus (hpv) dna replication: linker substitution analysis of the hpv type 11 origin. | papillomavirus dna replication requires the viral trans-acting factors e1 and e2 in addition to the host cell's general replication machinery. the origins of dna replication in bovine and human papillomavirus genomes have been localized to a specific part of the upstream regulatory region (urr) which includes recognition sites for e1 and e2 proteins. to fine map cis-acting elements influencing human papillomavirus type 11 (hpv-11) dna replication and to determine the relative contributions of su ... | 1995 | 7815528 |
| [asymptomatic sexually transmitted infections among hiv-tested persons]. | we have prospectively determined the frequency of asymptomatic sexually transmitted diseases (std) among patients seeking an hiv-test. in 246 patients, we observed 32 cases (13%) of asymptomatic std, predominantly infections caused by chlamydia trachomatis or human papillomavirus. std screening is of significance among patients seeking an hiv-test. | 1994 | 7817414 |
| binding of the lxcxe insulin motif to a hexapeptide derived from retinoblastoma protein. | peptides corresponding to retinoblastoma protein (rb) fragment 649-654 (lfykkv) were tested for their ability to recognize the lxcxe sequence motif in human papilloma virus type 16e7 protein (hpv-16e7) encompassing e7 residues 21-26 (dlycye) and an identical motif in human insulin comprising insulin b-chain residues 16-21 (ylvcge), respectively. interaction between these complementary peptide sequences was observed by several approaches, including direct and competitive elisa as well as affinity ... | 1995 | 7818556 |
| the role of early colposcopy in the management of females with first episode anogenital warts. | 212 females attending a genitourinary medicine (gum) clinic with first episode anogenital warts were screened by cervical cytology and colposcopy/histology for the presence of cervical epithelial abnormalities in keeping with infection by the human papillomavirus (hpv infection) and/or cervical intraepithelial neoplasia (cin). the prevalence of cervical epithelial abnormalities detected by cervical cytology alone was 32%, rising to 56% after colposcopic examination. however, the majority of cerv ... | 1994 | 7819352 |
| [stable suppression of transcription of human papillomavirus type 18 (hpv 18) e6 and e7 genes in transformed rat fibroblasts: use of an antisense oligonucleotide to the e7 gene]. | | 1994 | 7820079 |
| hpv detection using "hot start" polymerase chain reaction in patients with oral cancer: a clinicopathological study of 64 patients. | we examined the incidence of human papillomavirus (hpv) in intraoral cancers from 64 patients as determined by the highly sensitive technique of "hot start" polymerase chain reaction (pcr) in formalin-fixed paraffin-embedded tissues. polymerase-chain-reaction-amplified hpv dna was detected in the carcinomas of 16 patients (25%). the percentage of men in the hpv-positive (hpv+) group was greater than that in the hpv-negative (hpv-) group (86% versus 68%), but the difference was not statistically ... | 1994 | 7824504 |
| role of hiv as cofactor in hpv oncogenesis: in vitro evidences of virus interactions. | | 1994 | 7826030 |
| detection of dna and e7 transcripts of human papillomavirus types 16, 18, 31 and 33, tgf beta and gm-csf transcripts in cervical cancers and precancers. | the association of human papillomavirus (hpv) with a high proportion of cervical cancers should allow the efficiency of cytological screening methods to be improved. we report here that quantitative detection of hpv types 16, 18, 31 and 33 dna and the corresponding e7 transcripts by polymerase chain reaction (pcr) may be of value in identifying precancers and cancers. in clinical specimens with major cervical lesions, the level of e7 transcription does not appear to be related to concomitant tra ... | 1994 | 7826216 |
| [cervical pathology and immunodepression]. | the human papilloma virus is often involved in the pathogenesis of cervical lesions. a local or systemic immunodeficiency allows neoplasia outbreaks. we do not know if immunodepression only allows the virus to persist, or if the hpv induces a local immunodeficiency. large warts are often associated with pregnancy, but cervical cancers are not increased in pregnant women. induced immunodeficiency (among transplanted patients), or aids increase the rate of cin and cervical cancers. the more seriou ... | 1994 | 7827639 |
| human papillomavirus vaccines: a warty problem. | infection of the genital tract with human papillomaviruses (hpvs) is a common occurrence, and manifestations can include genital warts (condyloma acuminata), dysplasia, and invasive cancer. approaches to diagnose and treat hpv infections are costly and are not fully effective. even in populations at low risk for sexually transmitted diseases (stds), hpv infection is severalfold more prevalent than all other stds combined, and worldwide, uterine cervical cancer remains the most common cancer in w ... | 1994 | 7827787 |
| genetic characterization of the human papillomavirus (hpv) 18 e2 gene in clinical specimens suggests the presence of a subtype with decreased oncogenic potential. | hpv 18 is associated with 2 divergent phenotypes: (i) aggressive cervical cancer and a preponderance of cancer relative to cervical intra-epithelial neoplasia (cin) and (ii) benign warty lesions of the cervix. the e2 gene of hpv 18 encodes a regulatory protein that represses viral oncogene transcription and is involved in viral replication. variation within the e2 gene of hpv 18 and its correlation with the morphologic grade of associated lesions were analyzed in a sample of 20 hpv 18-positive c ... | 1995 | 7829247 |
| differential response of cervical intraepithelial and cervical carcinoma cell lines to transforming growth factor-beta 1. | transforming growth factor-beta 1 (tgf-beta 1) is a potent inhibitor of epithelial cell proliferation. it has been proposed that loss of sensitivity to growth inhibition by tgf-beta 1 may be an important step in the development of cervical carcinoma, but it remains unclear whether this represents an early or a late event. we compared the sensitivity to tgf-beta 1 of nontumorigenic human papillomavirus deoxyribonucleic acid (hpv dna)-positive cell lines derived from cervical intraepithelial neopl ... | 1994 | 7835777 |
| intra- and extracellular control mechanisms of human papillomavirus infection. | | 1994 | 7843929 |
| epidemiology: a tool for the study of human papillomavirus-related carcinogenesis. | | 1994 | 7843932 |
| [inverted papilloma and its association with human papillomavirus (hpv). a study with polymerase chain reaction (pcr)]. | nasal inverted papilloma is usually a benign tumor but is associated with squamous cell carcinoma in about 10% of cases. to determine the etiological role of human papillomavirus (hpv) in inverted papilloma and to clarify the relationship between the different types of human papillomavirus and malignant transformation, we analyzed retrospectively a series of 29 formalin - fixed, paraffin-embedded cases, 3 of which had squamous cell carcinoma. a highly sensitive and specific modification of the p ... | 1994 | 7843998 |
| rare association of human papillomavirus dna with esophageal cancer in japan. | to examine whether human papillomavirus (hpv) dna is associated with esophageal cancer, frozen and paraffin-embedded neoplasms of the upper aerodigestive tract, including esophageal cancer, were investigated. dna obtained from frozen specimens and cell lines were analyzed by both polymerase chain reaction (pcr) and southern blot hybridization. dna from paraffin-embedded samples were analyzed strictly by pcr. dna of hpv types 6 and 11 was detected in papillomas of the upper respiratory tract at > ... | 1995 | 7844381 |
| non-isotopic in situ hybridization of human papilloma virus on histologic sections: an amended protocol. | the authors report on their experience with an hpv non-radioactive in situ hybridization kit and describe the favorable results gained with the amended protocol, which are as follows: 1. the application of a decreased amount of both the probe and the chromogen substrate did not alter the quality of reactions. therefore we were able to make 60 reactions instead of the originally suggested 21. 2. the proteolytic enzyme digestion time could be prolonged by changing proteinase-k for pepsin which int ... | 1994 | 7847839 |
| screening for cervical abnormalities in women with anogenital warts in an std clinic: an inappropriate use of colposcopy. | an audit of the use of colposcopy in women with anogenital warts was performed. fifty women attending a clinic for sexually transmitted diseases in a district general hospital with anogenital warts were examined by cervical cytology and colposcopy for cervical infection by human papillomavirus (hpv) or epithelial abnormality indicating cervical intraepithelial neoplasia (cin) or both. collated results showed a high prevalence of both conditions in these 50 women; 20 (40%) had evidence of cervica ... | 1994 | 7849124 |
| expression of p53 protein in laryngeal squamous cell carcinoma and dysplasia: possible correlation with human papillomavirus infection and clinicopathological findings. | in order to evaluate the expression of p53 protein in 28 premalignant and 40 malignant squamous cell proliferations of the larynx and its relationship to tobacco consumption, human papillomavirus infection and differentiation grade of the lesions, p53 expression was examined by means of a microwave post-fixation immunohistochemical method using the pab 240 and pab 1801 monoclonal antibodies. hpv infection was assessed by non-isotopic in situ hybridization (nish) and polymerase chain reaction (pc ... | 1994 | 7850072 |
| detection of human papillomavirus rna in ovarian and endometrial carcinomas by reverse transcription/polymerase chain reaction. | the presence of human papillomavirus (hpv) type 16 and 18 transcripts was determined by reverse transcription/polymerase chain reaction (pcr) in ovarian and endometrial carcinoma tissue. hpv-16 dna sequences were detected in 50.0% (9/18) of the ovarian carcinomas and in 44.4% (8/18) of endometrial carcinomas. hpv-18 dna sequences were found in 16.7% (3/18) of both the ovarian and endometrial carcinomas. using rna-pcr analysis, we found 3 out of 9 (33.3%) of the hpv-16 dna-positive and 1 in 3 (33 ... | 1994 | 7851815 |
| degradation of p53 only is not sufficient for the growth stimulatory effect of human papillomavirus 16 e6 oncoprotein in human embryonic fibroblasts. | certain types of human papillomavirus (hpv), such as types 16 and 18, are thought to be responsible for the development of cervical carcinomas. the e6 and e7 genes of these viruses have transforming activities in various cultured cells and their mrnas and proteins are expressed in almost all cervical carcinoma cells. inactivation of the tumor suppressor p53 protein by the e6 gene is believed to be critical for transformation by these oncogenic hpvs. to determine whether degradation of the p53 pr ... | 1994 | 7852968 |
| vulvar intraepithelial neoplasia: age, morphological phenotype, papillomavirus dna, and coexisting invasive carcinoma. | recent studies suggest that subsets of vulvar intraepithelial neoplasia (vin) may be distinguished based on morphological presentation, the presence or absence of human papillomavirus (hpv) nucleic acids, and patient age. we analyzed 65 vin lesions, including 15 with associated squamous cell carcinoma, to determine the relationship between pathological parameters associated with common types of vin (multinucleation, koilocytosis, verruco-papillary morphology, diffuse atypia), rarer variants (dif ... | 1995 | 7860044 |
| a randomized trial of laser vaporization in the management of cervical intraepithelial neoplasia associated with human papilloma virus infection. | a randomized controlled trial was carried out at colposcopy clinics in the birmingham and midland hospital for women, to determine the efficacy of laser vaporization in the management of cervical human papilloma virus (hpv) infection occurring alone or in association with cervical intraepithelial neoplasia (cin), and to study the natural history of these lesions. the subjects were 365 women referred for investigation of cytological abnormality who were found to have histological evidence of hpv ... | 1993 | 7864916 |
| comparison of the polymerase chain reaction and southern blot analysis in detecting and typing human papilloma virus deoxyribonucleic acid in tumors of the lower female genital tract. | to conduct studies on the clinical and pathologic significance of human papilloma virus (hpv) in genital malignancies, accurate detection and typing of the virus in clinical material are essential. currently, southern blotting and the polymerase chain reaction (pcr) are two of the most commonly used methods to identify hpv. this study was undertaken to compare these techniques in the detection and typing of hpv in 242 invasive malignancies of the lower female genital tract. bamhi and psti restri ... | 1994 | 7866640 |
| [expression of hpv16e6 gene and preparation of monoclonal antibody against the expression product]. | the recombinant plasmid pas1-hpv16e6 containing the hpv16e6 gene was expressed in e. coli ar120 under nalidixic acid induction. a 19kd expression protein was isolated, purified and identified. mice were immunized with the purified e6 expression protein. a murine hybridoma, rac6, was obtained by fusing splenic cells from an immunized babl/c mouse with mouse myeloma cell line sp2/0-ag14 cells, followed by screening in hat medium, cloning and recloning in methyl cellulose. the hybridoma rac6 stably ... | 1994 | 7867098 |
| risk of acquiring human papillomavirus from the plume produced by the carbon dioxide laser in the treatment of warts. | the documented presence of human papillomavirus dna in the plume after carbon dioxide laser treatment of warts has raised questions about the risk of transmission of human papillomavirus to laser surgeons. | 1995 | 7868712 |
| detection of human papillomavirus types in cervical lesions of patients from taiwan by the polymerase chain reaction. | the association of human papillomavirus (hpv) infection with cervical carcinoma is well documented. the hpv types in cervical lesions of patients from taiwan are analyzed by the polymerase chain reaction (pcr). | 1994 | 7871443 |
| role of human papillomavirus in determining the hla associated risk of cervical carcinogenesis. | to investigate the role of human papillomavirus (hpv) in the association between hla dqw3 and squamous cell cancer of the cervix (sccc). | 1994 | 7876378 |
| absence of human papillomavirus in squamous cell carcinomas of nongenital skin from immunocompromised renal transplant patients: a comment. | | 1995 | 7880308 |
| human papillomavirus detection in genital lesions by in situ hybridization and ultrastructural observations. | detection of papillomavirus dna in situ hybridization technique was performed in 29 symptomatic patients (6 males and 23 females) during the period of 1989-1991 at the clinic for sexually transmitted diseases, universidade federal fluminense, state of rio de janeiro. all the male patients had condyloma acuminata. only hpv 6/11 were found in these lesions. clinical features in the female patients included vulvar condyloma acuminata, bowenoid papulosis, flat cervical condyloma, cervical condyloma ... | 1994 | 7885244 |
| micronutrients and cancer aetiology: the epidemiological evidence. | micronutrient deficiencies occur most commonly in poor countries and, therefore, are most likely to be associated with cancers common in these countries. epidemiological studies are hampered by inaccurate measurement of micronutrient intake and by the correlations between intakes of many nutrients. the strongest evidence for a protective effect of micronutrients is for oesophageal cancer. the identity of the micronutrients is not certain, but may include retinol, riboflavin, ascorbic acid and zn ... | 1994 | 7886059 |
| inhibition of growth of normal and human papillomavirus-transformed keratinocytes in monolayer and organotypic cultures by interferon-gamma and tumor necrosis factor-alpha. | the growth response of normal and human papillomavirus (hpv)-transformed cervical keratinocytes to interferon-gamma (ifn-gamma) and tumor necrosis factor-alpha was investigated in monolayer and organotypic raft cultures. the proliferation rates of monolayer cultures were assessed by [3h]tdr incorporation and fluorimetric dna titration. the growth of keratinocytes in organotypic cultures was estimated by their ability to stratify on collagen rafts and by immunohistochemistry for ki67 antigen expr ... | 1995 | 7887441 |
| human papillomavirus testing by hybrid capture appears to be useful in triaging women with a cytologic diagnosis of atypical squamous cells of undetermined significance. | our purpose was to determine the clinical value of human papillomavirus deoxyribonucleic acid testing with the hybrid capture test, specifically to examine whether human papillomavirus testing could identify which women with papanicolaou smears read as atypical squamous cells of undetermined significance were most likely to have histologically confirmed cervical intraepithelial neoplasia. | 1995 | 7892889 |
| possible role for human papillomavirus 16 in squamous cell carcinoma of the finger. | there are about 20 types of human papillomaviruses (hpvs) which are generally believed to be confined to the anogenital tract. hpv 16 is the most common of these. four squamous cell carcinomas of the finger from three individuals were analyzed for the presence of hpv dna by pst 1 restriction enzyme digest and southern blotting. under high stringency conditions, all were positive for hpv 16. the dna from each patient was digested with a panel of restriction enzymes so that integration of hpv into ... | 1994 | 7897367 |
| viral load as a determinant for transmission of human papillomavirus type 16 from mother to child. | whilst genital papillomaviruses are commonly believed to be sexually transmitted, transmission of human papillomavirus type 16 (hpv-16) from mother to child at delivery has been described previously [pakarian et al. (in press) british journal of obstetrics and gynaecology]. in order to determine whether viral load in cervical/vaginal cells was an important determinant of transmission 15 pregnant women with hpv-16 infections were studied. eight of these women had infants who were positive for hpv ... | 1994 | 7897374 |
| functional p53 protein in human papillomavirus-positive cancer cells. | there is accumulating evidence that the p53 protein contributes to tumor suppression by stimulating the transcription of specific cellular genes, such as the cell cycle control gene waf1/clp1. p53-mediated transcriptional activation is inhibited in cotransfection assays by overexpressed e6 protein from cancer-associated human papillomavirus (hpv) types, pointing at a possible molecular mechanism by which these viruses contribute to malignant cell transformation. here we analysed the transcriptio ... | 1995 | 7898934 |
| schistosomiasis and cervical cancer in ghana. | to discover a possible association between schistosomiasis and cervical dysplasia and/or cervical cancer in ghana, west africa. | 1993 | 7901060 |
| possible prodromes of human papillomavirus uterine cervix infection. | we present two case reports and mention four others with similar clinical, pathological and laboratory findings which suggest that viremia could possibly occur in some human papillomavirus (hpv) infections. fever, general indisposition or arthralgia associated with micturition difficulties or dysuria appear to be the symptoms accompanying this viremia. after a period of time these symptoms disappear and the infection shows typical hpv uterine cervix symptoms. the diagnosis of hpv infection was c ... | 1993 | 7901085 |
| interferon alpha-2b treatment of symptomatic chronic vulvodynia associated with koilocytosis. | in an open study with identical criteria for diagnosis, 16 female patients with typical symptoms of chronic vulvodynia associated with acetowhitening of the vestibular epithelium and koilocytosis in biopsy specimens received recombinant interferon alpha-2b 5 miu intralesionally 3 times weekly for 3 weeks or subcutaneously 3 times weekly for 8 weeks. three months after end of therapy, clinical symptoms had disappeared in 7 (70%) of the 10 patients treated intralesionally, compared with only one ( ... | 1993 | 7904410 |
| vulvar neoplasia associated with other primary malignancies. | it is recognized that a significant number of patients with vulvar neoplasia have had previous, concurrent, or subsequent genital tract neoplasia. this has resulted in speculation that there may be a common etiology and, in particular, of the possibility of an infection element. among 59 cases of vulvar cancer diagnosed at the national taiwan university hospital from 1976 to 1991, there were seven cases (12%) associated with other primary malignancies. carcinoma of the cervix was the most freque ... | 1993 | 7904858 |
| delayed-type hypersensitivity response to human papillomavirus type 16 e6 protein in a mouse model. | a mouse model incorporating the epitheliotropic nature of human papillomavirus (hpv) infections has been used to study an immune response to hpv type 16 (hpv-16) e6 protein in vivo. using a transplantation technique, a novel immortal keratinocyte cell line expressing the e6 protein has been grafted onto syngeneic mice to re-form a differentiated epithelium overlying a granulation tissue bed. by this approach the presentation of viral antigens to the immune system can be modelled in a way analogo ... | 1994 | 7906707 |
| cellular subsets and epithelial icam-1 and hla-dr expression in human papillomavirus infection of the vulva. | cryostat sections of 20 clinical condylomata of the vulva induced by human papillomavirus and 5 normal control biopsies were examined using immunohistochemistry. the results indicated that in vulvar papillomavirus infection the intraepithelial langerhans' cells showed abnormal morphology and a significantly lower density than controls. cd1a positive langerhans' cells were also observed in dermis of condylomata, suggesting an abnormal epithelial traffic of dendritic cells. t lymphocytes with a me ... | 1994 | 7908483 |
| disrupted dichotomous intracellular control of human papillomavirus infection in cancer of the cervix. | the role of specific human papillomaviruses (hpvs) in the aetiology of cancer of the cervix is firmly established. progression of an hpv-infected cell clone to invasive growth involves consecutive modifications of a set of host cell genes. some of these modifications suppress viral oncogene functions post-transcriptionally, and others suppress transcription via a signalling pathway stimulated by activated macrophages and possibly by additional cells. i describe a scheme that tries to unify avail ... | 1994 | 7909014 |
| induction of proliferating cell nuclear antigen in differentiated keratinocytes of human papillomavirus-infected lesions. | the expression of proliferating cell nuclear antigen (pcna) was studied in human papillomavirus (hpv)-infected, benign and malignant lesions of the genital tract and larynx using immunocytochemical staining of formalin-fixed clinical specimens. we observed the induction of pcna in squamous carcinomas and adenocarcinomas, as has been demonstrated with other malignancies. in addition, the differentiated keratinocytes of the upper spinous cells and granulocytes in condylomata acuminata and low-grad ... | 1994 | 7909306 |
| management of human papilloma virus vulvo-perineal infection with systemic beta-interferon and thymostimulin in hiv-positive patients. | to evaluate the effect of a combined therapy based on beta-interferon and thymostimulin in hiv-seropositive women with florid vulvoperineal condylomatosis. | 1994 | 7909764 |
| the p53 status of cultured human premalignant oral keratinocytes. | around 60% of oral squamous cell carcinomas (sccs) have been shown to harbour p53 mutations, and other studies have demonstrated mutant p53 genes in normal and dysplastic squamous epithelium adjacent to these sccs. in line with these earlier studies we show here that dok, a keratinocyte cell line derived from a dysplasia, displays elevated levels of p53 protein and harbours a 12 bp in-frame deletion of the p53 gene spanning codons 188-191. in contrast, the coding region of the p53 gene was norma ... | 1994 | 7917902 |
| genetic analysis of an in vitro model system for human papillomavirus type 16-associated tumorigenesis. | we have investigated the genetic basis for malignant progression of an human papillomavirus type 16 immortalized human keratinocyte cell line (hpkia) by somatic cell fusion. in this cell culture model system for human papillomavirus type 16-associated tumorigenesis, early passage cells (hpkia-nt) are nontumorigenic, whereas gamma-irradiated late passage cells (hpkia-t) are tumorigenic when tested in nude mice. fusion of hpkia-t cells with normal human keratinocytes or with hpkia-nt gave rise to ... | 1994 | 7923202 |
| differential disruption of genomic integrity and cell cycle regulation in normal human fibroblasts by the hpv oncoproteins. | genomic integrity is maintained by a network of cellular activities that assess the status of the genome at a given point in time, provide signals to proceed with or halt cell cycle progression, and provide for repair of damaged dna. mutations in any part of these pathways can have the ultimate effect of disturbing chromosomal integrity. recent work suggests that p53 performs this integrator function in mammalian cells. our present study demonstrates that in mortal cells, the expression of e6 an ... | 1994 | 7926757 |
| pcr-rflp studies on chromosome 3p in formaldehyde-fixed, paraffin-embedded cervical cancer tissues. | loss of heterozygosity (loh) has been extensively studied on the short arm of chromosome 3, and functional proofs have been obtained defining a tumor-suppressor locus at 3p21-22. we examined 31 paraffin-embedded cervical cancer samples for loh, using 5 pcr-primer pairs, located around 3p21. allele loss was found in 19 out of the 27 informative samples (70%) while 13 out of 23 informative samples (56%) had loh located at 3p21-22. more of the human papillomavirus (hpv)-positive samples had loh com ... | 1994 | 7927869 |
| detection of multiple human papillomavirus types in condylomata acuminata from immunosuppressed patients. | using the hybrid capture method, condylomata acuminata from healthy patients (controls) and patients with altered cell-mediated immunity were analyzed for multiple human papillomavirus (hpv) dna: 82.9% and 38.0% of lesions from 41 controls and 21 patients, respectively, were hpv dna-positive only with probes for low-risk hpv types (p = .00035). using probes for both low- and high-risk hpv types, 16.3% and 52.3% of lesions from 43 controls and 21 patients, respectively, were positive for both pro ... | 1994 | 7930715 |
| analysis of p53 status in tonsillar carcinomas associated with human papillomavirus. | tonsillar squamous cell carcinomas (a total of 14) were examined both for the presence of human papillomavirus (hpv) dna and for p53 alterations. general primer-mediated hpv polymerase chain reaction (gp-pcr) revealed the presence of hpv dna in 12/14 cases. subsequent typing by hpv type-specific pcr and sequence or hybridization analysis of gp-pcr products revealed dna from hpv 16 in seven cases, from hpv 33 in two cases, and from hpv 7, hpv 16/33 and hpv 33/59 each in a single case. p53 immunoh ... | 1994 | 7931165 |
| efficacy of co2 laser surgery in treating squamous intraepithelial lesions. an analysis of clinical and virologic results. | this paper reports the clinical and virologic results of co2 laser treatment for squamous intraepithelial lesions in 154 patients. on the basis of cervical canal involvement, vaporization or conization was performed. failure rates following the first and second treatments were 4.5% and 0%, respectively. however, human papillomavirus persistence in several women with completely normal follow-up suggests that one of the possible mechanisms of failure could be reexposure of the healing area to a vi ... | 1994 | 7932396 |