| agrobacterium tumefaciens-mediated transformation of pseudocercospora fijiensis to determine the role of pfhog1 in osmotic stress regulation and virulence modulation. | black sigatoka disease, caused by pseudocercospora fijiensis is a serious constraint to banana production worldwide. the disease continues to spread in new ecological niches and there is an urgent need to develop strategies for its control. the high osmolarity glycerol (hog) pathway in saccharomyces cerevisiae is well known to respond to changes in external osmolarity. hog pathway activation leads to phosphorylation, activation and nuclear transduction of the hog1 mitogen-activated protein kinas ... | 2017 | 28559879 |
| media development for large scale agrobacterium tumefaciens culture. | a chemically defined media was developed for growing agrobacterium tumefaciens at large scale for commercial production of recombinant proteins by transient expression in plants. design of experiments was used to identify major and secondary effects of ten media components: sucrose, ammonium sulfate ((nh4 )2 so4 ), magnesium sulfate heptahydrate (mgso4 *7h2 o), calcium chloride dihydrate (cacl2 *2h2 o), iron (ii) sulfate heptahydrate (feso4 *7h2 o), manganese (ii) sulfate monohydrate (mnso4 *h2 ... | 2017 | 28556626 |
| thermostabilization of the uronate dehydrogenase from agrobacterium tumefaciens by semi-rational design. | aldaric acids represent biobased 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. until today they are mostly produced from corresponding aldoses using strong chemical oxidizing agents. an environmentally friendly and more selective process could be achieved by using natural resources such as seaweed or pectin as raw material. these contain large amounts of uronic acids as major constituents such as glucuronic acid and galacturonic acid which can be ... | 2017 | 28545260 |
| complementation of ctb7 in the maize pathogen cercospora zeina overcomes the lack of in vitro cercosporin production. | gray leaf spot (gls), caused by the sibling species cercospora zeina or cercospora zeae-maydis, is cited as one of the most important diseases threatening global maize production. c. zeina fails to produce cercosporin in vitro and, in most cases, causes large coalescing lesions during maize infection, a symptom generally absent from cercosporin-deficient mutants in other cercospora spp. here, we describe the c. zeina cercosporin toxin biosynthetic (ctb) gene cluster. the oxidoreductase gene ctb7 ... | 2017 | 28535078 |
| crispr/cas9-mediated targeted mutagenesis of gmft2a delays flowering time in soya bean. | flowering is an indication of the transition from vegetative growth to reproductive growth and has considerable effects on the life cycle of soya bean (glycine max). in this study, we employed the crispr/cas9 system to specifically induce targeted mutagenesis of gmft2a, an integrator in the photoperiod flowering pathway in soya bean. the soya bean cultivar jack was transformed with three sgrna/cas9 vectors targeting different sites of endogenous gmft2a via agrobacterium tumefaciens-mediated tran ... | 2017 | 28509421 |
| common structural elements in the chromophore binding pocket of the pfr state of bathy phytochromes. | phytochromes are bimodal photoreceptors which, upon light absorption by the tetrapyrrole chromophore, can be converted between a red-absorbing state (pr) and far-red-absorbing state (pfr). in bacterial phytochromes, either pr or pfr are the thermally stable states, thereby constituting the classes of prototypical and bathy phytochromes, respectively. in this work, we have employed vibrational spectroscopies to elucidate the origin of the thermal stability of the pfr states in bathy phytochromes. ... | 2017 | 28500706 |
| atomic force microscopy correlates antimetastatic potentials of hepg2 cell line with its redox/energy status: effects of curcumin and khaya senegalensis. | the fatality of cancer is mostly dependent on the possibility of occurrence of metastasis. thus, if the development of metastasis can be prevented through novel therapeutic strategies targeted against this process, then the success of cancer treatment will drastically increase. in this study, therefore, we evaluated the antimetastatic potentials of an extract of khaya senegalensis and curcumin on the metastatic liver cell line hepg2, and also assessed the anticancer property of the extract. | 2017 | 28494852 |
| heritable genomic fragment deletions and small indels in the putative engase gene induced by crispr/cas9 in barley. | targeted genome editing with the crispr/cas9 system has been used extensively for the selective mutation of plant genes. here we used crispr/cas9 to disrupt the putative barley (hordeum vulgare cv. "golden promise") endo-n-acetyl-β-d-glucosaminidase (engase) gene. five single guide rnas (sgrnas) were designed for different target sites in the upstream part of the engase coding region. targeted fragment deletions were induced by co-bombarding selected combinations of sgrna with wild-type cas9 usi ... | 2017 | 28487703 |
| abp9, a maize bzip transcription factor, enhances tolerance to salt and drought in transgenic cotton. | abp9 , encoding a bzip transcription factor from maize, enhances tolerance to multiple stresses and may participate in the aba signaling pathway in transgenic cotton by altering physiological and biochemical processes and stress-related gene expression. abiotic stresses, such as soil salinity and drought, negatively affect growth, development, and yield in cotton. gene abp9, which encodes a bzip transcription factor, binds to the abscisic acid (aba)-responsive-element (abre2) motif of the maize ... | 2017 | 28474114 |
| functional interaction and structural characteristics of unique components of helicobacter pylori t4ss. | the helicobacter pylori infection of the human gastric mucosa causes chronic active gastritis and peptic ulcers and is associated with the development of gastric cancer. epidemiological studies show that these gastric diseases are related to virulent h. pylori strains that harbor the cytotoxin-associated gene pathogenicity island (cag pai). the cag pai is a dna insertion in the h. pylori chromosome that encodes ~ 27 proteins, including the oncoprotein caga. approximately 20 of these proteins hav ... | 2017 | 28470874 |
| use of the yeast-like cells of tremella fuciformis as a cell factory to produce a pleurotus ostreatus hydrophobin. | to obtain hydrophobin, a class i hydrophobin gene, po.hyd from pleurotus ostreatus, was transformed into the yeast-like cells of tremella fuciformis using agrobacterium tumefaciens. | 2017 | 28470627 |
| senescence is delayed when ramie (boehmeria nivea l.) is transformed with the isopentyl transferase (ipt) gene under control of the sag12 promoter. | ramie is an economically important industrial fiber crop widely planted in china, india, and other southeast asian and pacific rim countries. it plays an important role in china's economy, where ramie farming, industry, and trade provide livelihood support to about five million people. however, poor fiber production resulting from leaf senescence and leaf abscission is a significant problem. in this study, we report the successful production of transgenic ramie plants which delayed leaf senescen ... | 2017 | 28469976 |
| a new and efficient approach for construction of uridine/uracil auxotrophic mutants in the filamentous fungus aspergillus oryzae using agrobacterium tumefaciens-mediated transformation. | aspergillus oryzae is a filamentous fungus widely used in food industry and as a microbial cell factory for recombinant protein production. due to the inherent resistance of a. oryzae to common antifungal compounds, genetic transformation of this mold usually requires auxotrophic mutants. in this study, we show that agrobacterium tumefaciens-mediated transformation (atmt) method is very efficient for deletion of the pyrg gene in different aspergillus oryzae wild-type strains to generate uridine/ ... | 2017 | 28466303 |
| water-wisteria as an ideal plant to study heterophylly in higher aquatic plants. | the semi-aquatic plant water-wisteria is suggested as a new model to study heterophylly due to its many advantages and typical leaf phenotypic plasticity in response to environmental factors and phytohormones. water-wisteria, hygrophila difformis (acanthaceae), is a fast growing semi-aquatic plant that exhibits a variety of leaf shapes, from simple leaves to highly branched compound leaves, depending on the environment. the phenomenon by which leaves change their morphology in response to enviro ... | 2017 | 28466187 |
| establishment of a gene function analysis system for the euhalophyte salicornia europaea l. | a salicornia europaea l. in vitro cell transformation system was developed and further applied to senhx1 function investigation. the exploration of salt-tolerant genes from halophyte has seriously been limited by the lack of self-dependent transformation system. here, an agrobacterium tumefaciens-mediated in vitro cell transformation system of euhalophyte salicornia europaea l. was developed. calli derived from hypocotyl of s. europaea were co-cultured for 3 days with agrobacterium at od600 rang ... | 2017 | 28466186 |
| improvement for agronomically important traits by gene engineering in sweetpotato. | sweetpotato is the seventh most important food crop in the world. it is mainly used for human food, animal feed, and for manufacturing starch and alcohol. this crop, a highly heterozygous, generally self-incompatible, outcrossing polyploidy, poses numerous challenges for the conventional breeding. its productivity and quality are often limited by abiotic and biotic stresses. gene engineering has been shown to have the great potential for improving the resistance to these stresses as well as the ... | 2017 | 28465664 |
| bacterial pero permeases transport sulfate and related oxyanions. | rhodobacter capsulatus synthesizes the high-affinity abc transporters cystwa and modabc to specifically import the chemically related oxyanions sulfate and molybdate, respectively. in addition, r. capsulatus has the low-affinity permease pero acting as a general oxyanion transporter, whose elimination increases tolerance to molybdate and tungstate. although pero-like permeases are widespread in bacteria, their function has not been examined in any other species to date. here, we present evidence ... | 2017 | 28461447 |
| use of chimeric type iv secretion systems to define contributions of outer membrane subassemblies for contact-dependent translocation. | recent studies have shown that conjugation systems of gram-negative bacteria are composed of distinct inner and outer membrane core complexes (imcs and omccs, respectively). here, we characterized the omcc by focusing first on a cap domain that forms a channel across the outer membrane. strikingly, the omcc caps of the escherichia coli pkm101 tra and agrobacterium tumefaciens virb/vird4 systems are completely dispensable for substrate transfer, but required for formation of conjugative pili. the ... | 2017 | 28452085 |
| the abundance of endofungal bacterium rhizobium radiobacter (syn. agrobacterium tumefaciens) increases in its fungal host piriformospora indica during the tripartite sebacinalean symbiosis with higher plants. | rhizobium radiobacter (syn. agrobacterium tumefaciens, syn. "agrobacterium fabrum") is an endofungal bacterium of the fungal mutualist piriformospora (syn. serendipita) indica (basidiomycota), which together form a tripartite sebacinalean symbiosis with a broad range of plants. r. radiobacter strain f4 (rrf4), isolated from p. indica dsm 11827, induces growth promotion and systemic resistance in cereal crops, including barley and wheat, suggesting that r. radiobacter contributes to a successful ... | 2017 | 28450855 |
| silencing of agrobacterium tumefaciens oncogenes ipt and iaam induces resistance to crown gall disease in plum but not in apricot. | in this study, two vectors with short-length chimeric transgenes were used to produce prunus rootstocks resistant to crown gall disease through rna-interference-mediated gene silencing of the agrobacterium tumefaciens oncogenes ipt and iaam. | 2017 | 28449201 |
| expression of pennisetum glaucum eukaryotic translational initiation factor 4a (pgeif4a) confers improved drought, salinity, and oxidative stress tolerance in groundnut. | eukaryotic translational initiation factor 4a belong to family of helicases, involved in multifunctional activities during stress and non-stress conditions. the eif4a gene was isolated and cloned from semi-arid cereal crop of pennisetum glaucum. in present study, the pgeif4a gene was expressed under the regulation of stress inducible arabidopsis rd29a promoter in groundnut (cv jl-24) with bar as a selectable marker. the de-embryonated cotyledons were infected with agrobacterium tumefaciens (lba4 ... | 2017 | 28439277 |
| characterization the carotenoid productions and profiles of three rhodosporidium toruloides mutants from agrobacterium tumefaciens-mediated transformation. | the red yeast rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. however, it remains challenging to study its carotenoid production profiles owing to limited biochemical information and inefficient genetic tools. here we used an agrobacterium tumefaciens-mediated transformation (atmt) to change its carotenoid production and profiles. we constructed r. toruloides np11 mutant libraries with atmt, selected three mutants wit ... | 2017 | 28426167 |
| dissecting virulence function from recognition: cell death suppression in nicotiana benthamiana by xopq/hopq1-family effectors relies on eds1-dependent immunity. | many gram-negative plant pathogenic bacteria express effector proteins of the xopq/hopq1 family which are translocated into plant cells via the type iii secretion system during infection. in nicotiana benthamiana, recognition of xopq/hopq1 proteins induces an effector-triggered immunity (eti) reaction which is not associated with strong cell death but renders plants immune against pseudomonas syringae and xanthomonas campestris pv. vesicatoria strains. additionally, xopq suppresses cell death in ... | 2017 | 28423458 |
| elevated auxin and reduced cytokinin contents in rootstocks improve their performance and grafting success. | plant grafting is an important technique for horticultural and silvicultural production. however, many rootstock plants suffer from undesirable lateral bud outgrowth, low grafting success rates or poor rooting. here, we used a root-predominant gene promoter (sbugt) to drive the expression of a tryptophan-2-monooxygenase gene (iaam) from agrobacterium tumefaciens to increase auxin levels in tobacco. the transgenic plants, when used as a rootstock, displayed inhibited lateral bud outgrowth, enhanc ... | 2017 | 28376249 |
| enhancing the biocatalytic manufacture of the key intermediate of atorvastatin by focused directed evolution of halohydrin dehalogenase. | halohydrin dehalogenases (hhdhs) are biocatalytically interesting enzymes due to their ability to form c-c, c-n, c-o, and c-s bonds. one of most important application of hhdh was the protein engineering of hhec (halohydrin dehalogenase from agrobacterium radiobacter ad1) for the industrial manufacturing of ethyl (r)-4-cyano-3-hydroxybutanoate (hn), a key chiral synthon of a cholesterol-lowering drug of atorvastatin. during our development of an alternative, more efficient and economic route for ... | 2017 | 28165015 |
| exploring the thermostable properties of halohydrin dehalogenase from agrobacterium radiobacter ad1 by a combinatorial directed evolution strategy. | as a crucial factor for biocatalysts, protein thermostability often arises from a combination of factors that are often difficult to rationalize. in this work, the thermostable nature of halohydrin dehalogenase from agrobacterium radiobacter ad1 (hhec) was systematically explored using a combinatorial directed evolution approach. for this, a mutagenesis library of hhec mutants was first constructed using error-prone pcr with low mutagenesis frequency. after screening approximately 2000 colonies, ... | 2017 | 28074221 |
| covalent immobilization of agrobacterium radiobacter epoxide hydrolase on ethylenediamine functionalised epoxy supports for biocatalytical synthesis of (r)-epichlorohydrin. | to improve the operational stability and reusability of an epoxide hydrolase (eh) for the biosynthesis of optically active epoxides. | 2016 | 27379653 |
| proteobacteria from the human skin microbiota: species-level diversity and hypotheses. | the human skin microbiota is quantitatively dominated by gram-positive bacteria, detected by both culture and metagenomics. however, metagenomics revealed a huge variety of gram-negative taxa generally considered from environmental origin. for species affiliation of bacteria in skin microbiota, clones of 16s rrna gene and colonies growing on diverse culture media were analyzed. species-level identification was achieved for 81% of both clones and colonies. fifty species distributed in 26 genera w ... | 2016 | 28616476 |
| improvement of the thermostability and activity of halohydrin dehalogenase from agrobacterium radiobacter ad1 by engineering c-terminal amino acids. | in the current study, a three-tiered mutagenesis strategy was employed to simultaneously improve the thermostability and activity of halohydrin dehalogenase from agrobacterium radiobacter ad1 (hhec) by engineering the last ten amino acids (met245∼glu254) of its c-terminal region. initially, truncated mutagenesis results displayed that c-terminal deletions decreased the thermostability and/or activity of hhec. then ten residues were subjected to single-site saturation mutagenesis, resulting in 20 ... | 2015 | 26302840 |
| mdc-analyzer-facilitated combinatorial strategy for improving the activity and stability of halohydrin dehalogenase from agrobacterium radiobacter ad1. | halohydrin dehalogenase from agrobacterium radiobacter ad1 (hhec) displays a broad substrate range with high regio- and enantioselectivity of both ring-closure and ring-opening reactions, making the enzyme a useful catalyst for the production of optically pure epoxides and β-substituted alcohols. in this study, we report a novel method using an mdc-analyzer-facilitated combinatorial strategy to improve the activity and stability of hhec by simultaneously randomizing multiple contiguous residues. ... | 2015 | 25896949 |
| exploring the enantioselective mechanism of halohydrin dehalogenase from agrobacterium radiobacter ad1 by iterative saturation mutagenesis. | halohydrin dehalogenase from agrobacterium radiobacter ad1 (hhec) shows great potential in producing valuable chiral epoxides and β-substituted alcohols. the wild-type (wt) enzyme displays a high r-enantiopreference toward most aromatic substrates, whereas no s-selective hhec has been reported to date. to obtain more enantioselective enzymes, seven noncatalytic active-site residues were subjected to iterative saturation mutagenesis (ism). after two rounds of screening aspects of both activity an ... | 2015 | 25681194 |
| a single point mutation enhances hydroxynitrile synthesis by halohydrin dehalogenase. | the cyanide-mediated ring opening of epoxides catalyzed by halohydrin dehalogenases yields β-hydroxynitriles that are of high interest for synthetic chemistry. the best studied halohydrin dehalogenase to date is the enzyme from agrobacterium radiobacter, but this enzyme (hhec) exhibits only low cyanolysis activities. sequence comparison between a pair of related halohydrin dehalogenases from corynebacterium and mycobacterium suggested that substitution of a threonine that interacts with the acti ... | 2015 | 25659632 |
| triesterase and promiscuous diesterase activities of a di-co(ii)-containing organophosphate degrading enzyme reaction mechanisms. | the reaction mechanism for the hydrolysis of trimethyl phosphate and of the obtained phosphodiester by the di-co(ii) derivative of organophosphate degrading enzyme from agrobacterium radiobacter p230(opda), have been investigated at density functional level of theory in the framework of the cluster model approach. both mechanisms proceed by a multistep sequence and each catalytic cycle begins with the nucleophilic attack by a metal-bound hydroxide on the phosphorus atom of the substrate, leading ... | 2015 | 25582757 |
| dc-analyzer-facilitated combinatorial strategy for rapid directed evolution of functional enzymes with multiple mutagenesis sites. | iterative saturation mutagenesis (ism) has been shown to be a powerful method for directed evolution. in this study, the approach was modified (termed m-ism) by combining the single-site saturation mutagenesis method with a dc-analyzer-facilitated combinatorial strategy, aiming to evolve novel biocatalysts efficiently in the case where multiple sites are targeted simultaneously. initially, all target sites were explored individually by constructing single-site saturation mutagenesis libraries. n ... | 2014 | 25449543 |
| rhizobium radiobacter peritonitis: the first case report from india and review. | rhizobium radiobacter is an opportunistic human pathogen in debilitated patients with foreign plastic intravascular devices and peritoneal dialysis (pd) catheters. we performed a medline search of the english-language literature on r. radiobacter continuous ambulatory peritoneal dialysis (capd) peritonitis in end-stage renal disease (esrd) and reviewed 13 cases. | 2014 | 28663815 |
| rapid regeneration and ploidy stability of 'cv ir36' indica rice (oryza sativa. l) confers efficient protocol for in vitro callus organogenesis and agrobacterium tumefaciens mediated transformation. | cereal crops are the major targets for transformation mediated crop improvement and ir36 is an early maturing, high yielding, insect and disease resistant rice variety however, it is abiotic stress sensitive. hence, development of an efficient and reproducible micropropagation system via somatic embryogenesis and agrobacterium tumefaciens mediated transformation is prerequisite to develop abiotic stress tolerant ir36. further, genetic stability of analysis of plantlets through rapd and issr and ... | 2013 | 28510899 |
| highly efficient synthesis of (r)-3-quinuclidinol in a space-time yield of 916 g l(-1) d(-1) using a new bacterial reductase arqr. | a new keto reductase (arqr), identified from agrobacterium radiobacter ecu2556, can efficiently reduce 3-quinuclidinone in excellent enantioselectivity and high space-time yield for the synthesis of (r)-3-quinuclidinol, a chiral building block of many antimuscarinic agents. this is the first time that a high yield of (r)-3-quinuclidinol up to 916 g l(-1) d(-1) using a bioreduction approach is reported. | 2013 | 24050153 |
| high frequency regeneration via direct somatic embryogenesis and efficient agrobacterium-mediated genetic transformation of tobacco. | a direct somatic embryogenesis protocol was developed for four cultivars of nicotiana species, by using leaf disc as an explant. direct somatic embryogenesis of nicotiana by using bap and iaa has not been investigated so far. this method does not require formation of callus tissues which leads to somaclonal variations. the frequency of somatic embryogenesis was strongly influenced by the plant growth hormones. the somatic embryos developing directly from explant tissue were noticed after 6 d of ... | 2013 | 23518589 |
| quantum chemical modeling of the dehalogenation reaction of haloalcohol dehalogenase. | the dehalogenation reaction of haloalcohol dehalogenase hhec from agrobacterium radiobacter ad1 was investigated theoretically using hybrid density functional theory methods. hhec catalyzes the enantioselective conversion of halohydrins into their corresponding epoxides. the reaction is proposed to be mediated by a catalytic ser132-tyr145-arg149 triad, and a distinct halide binding site is suggested to facilitate halide displacement by stabilizing the free ion. we investigated the hhec-mediated ... | 2008 | 26636366 |
| technical communication: a simple and economically viable medium for the growth of agrobacterium radiobacter for the production of d-amino acid. | a simple and cost effective medium for the growth and d-amino acid production by agrobacterium radiobacter from hydantoin is reported. the effectiveness of this medium is compared with other synthetic media. | 1996 | 24415435 |