| differential detection of pathogenic yersinia spp. by fluorescence in situ hybridization. | yersinia enterocolitica, y. pseudotuberculosis and y. pestis are pathogens of major medical importance, which are responsible for a considerable number of infections every year. the detection of these species still relies on cultural methods, which are slow, labour intensive and often hampered by the presence of high amounts of accompanying flora. in this study, fluorescence in situ hybridization (fish) was used to develop a fast, sensitive and reliable alternative to detect viable bacteria in f ... | 2017 | 27889163 |
| green fluorescent protein labeling of food pathogens yersinia enterocolitica and yersinia pseudotuberculosis. | labeling of bacteria with marker genes, such as green fluorescent protein, is a useful and practicable tool for tracking and enumerating bacterial cells in a complex environment e.g. discrimination from the indigenous background population. in this study, novel turbogfp prokaryotic expression vector was utilized for labeling of yersinia species. y. enterocolitica biovar 1a, biovar 2, biovar 4 and y. pseudotuberculosis were successfully transformed with the vector and expressed bright green fluor ... | 2017 | 27838541 |
| detection of pathogenic yersinia enterocolitica in pet djungarian hamsters in japan. | the prevalence of yersinia enterocolitica (y. enterocolitica) and yersinia pseudotuberculosis was examined in 151 pet animals including 108 rodents, 39 rabbits and four sugar gliders from 13 pet stores in the yamaguchi prefecture, japan. y. enterocolitica serogroup o:3 biotype 3 negative for the voges-proskauer reaction (o:3/3 variant vp-) was isolated from five djungarian hamsters (phodopus sungorus) raised at the same pet store. these pathogenic y. enterocolitica isolates carried the virulence ... | 2016 | 27396397 |
| vitronectin binds to a specific stretch within the head region of yersinia adhesin a and thereby modulates yersinia enterocolitica host interaction. | complement resistance is an important virulence trait of yersinia enterocolitica (ye). the predominant virulence factor expressed by ye is yersinia adhesin a (yada), which enables bacterial attachment to host cells and extracellular matrix and additionally allows the acquisition of soluble serum factors. the serum glycoprotein vitronectin (vn) acts as an inhibitory regulator of the terminal complement complex by inhibiting the lytic pore formation. here, we show yada-mediated direct interaction ... | 2017 | 27798934 |
| evaluation of chromogenic medium for selective isolation of yersinia. | cefsulodin-irgasan-novobiocin agar (cin) has been used as a selective agar to detect yersinia in food or human patients; however, its components can inhibit the growth of some strains of yersinia enterocolitica serovar o3 and y. pseudotuberculosis. recently, a new yersinia selective agar, chromagar yersinia enterocolitica (caye), was developed and evaluated as a novel selective agar for pathogenic y. enterocolitica. in this research, a total of 251yersinia strains (176 pathogenic y. enterocoliti ... | 2016 | 27784868 |
| [acute bacterial aortitis by yersinia enterocolitica]. | | 2016 | 26778100 |
| the positron emission tomography tracer 3'-deoxy-3'-[18f]fluorothymidine ([18f]flt) is not suitable to detect tissue proliferation induced by systemic yersinia enterocolitica infection in mice. | most frequently, gram-negative bacterial infections in humans are caused by enterobacteriaceae and remain a major challenge in medical diagnostics. we non-invasively imaged moderate and severe systemic yersinia enterocolitica infections in mice using the positron emission tomography (pet) tracer 3'-deoxy-3'-[18f]fluorothymidine ([18f]flt), which is a marker of proliferation, and compared the in vivo results to the ex vivo biodistributions, bacterial loads, and histologies of the corresponding or ... | 2016 | 27701464 |
| interactions between the cytoplasmic domains of pspb and pspc silence the yersinia enterocolitica phage shock protein response. | the phage shock protein (psp) system is a widely conserved cell envelope stress response that is essential for the virulence of some bacteria, including yersinia enterocolitica recruitment of pspa by the inner membrane pspb-pspc complex characterizes the activated state of this response. the pspb-pspc complex has been proposed to be a stress-responsive switch, changing from an off to an on state in response to an inducing stimulus. in the off state, pspa cannot access its binding site in the c-t ... | 2016 | 27698088 |
| detection of yersinia enterocolitica serotype o:9 in the faeces of cattle with false positive reactions in serological tests for brucellosis in ireland. | intestinal infection by yersinia enterocolitica serotype o:9 (yeo9) in cattle has been linked to false positive serological reactivity (fpsr) in diagnostic tests for brucellosis. although eradicated in ireland, brucellosis monitoring still identifies seropositive animals, usually one or two (termed singletons) per herd, which are classed as fpsr. to investigate a link between fpsr and yeo9, faeces and blood were collected from singleton fpsr cattle, and from companion animals, in eight selected ... | 2016 | 27687940 |
| national outbreak of yersinia enterocolitica infections in military and civilian populations associated with consumption of mixed salad, norway, 2014. | in may 2014, a cluster of yersinia enterocolitica (ye) o9 infections was reported from a military base in northern norway. concurrently, an increase in ye infections in civilians was observed in the norwegian surveillance system for communicable diseases. we investigated to ascertain the extent of the outbreak and identify the source in order to implement control measures. a case was defined as a person with laboratory-confirmed ye o9 infection with the outbreak multilocus variable-number tandem ... | 2016 | 27588690 |
| yersinia enterocolitica yoph-deficient strain activates neutrophil recruitment to peyer's patches and promotes clearance of the virulent strain. | yersinia enterocolitica evades the immune response by injecting yersinia outer proteins (yops) into the cytosol of host cells. yoph is a tyrosine phosphatase critical for yersinia virulence. however, the mucosal immune mechanisms subverted by yoph during in vivo orogastric infection with y. enterocolitica remain elusive. the results of this study revealed neutrophil recruitment to peyer's patches (pp) after infection with a yoph-deficient mutant strain (y. enterocolitica δyoph). while the y. ent ... | 2016 | 27550935 |
| conventional and molecular methods used in the detection and subtyping of yersinia enterocolitica in food. | | 2016 | 27543816 |
| a new analytical platform based on field-flow fractionation and olfactory sensor to improve the detection of viable and non-viable bacteria in food. | an integrated sensing system is presented for the first time, where a metal oxide semiconductor sensor-based electronic olfactory system (mos array), employed for pathogen bacteria identification based on their volatile organic compound (voc) characterisation, is assisted by a preliminary separative technique based on gravitational field-flow fractionation (grfff). in the integrated system, a preliminary step using grfff fractionation of a complex sample provided bacteria-enriched fractions read ... | 2016 | 27520323 |
| detection, seroprevalence and antimicrobial resistance of yersinia enterocolitica and yersinia pseudotuberculosis in pig tonsils in northern italy. | yersiniosis is the third most common reported zoonoses in europe, with y. enterocolitica and y. pseudotuberculosis responsible for 98.66% and 0.94% of the confirmed human cases in 2013. from june 2013 to october 2014, 201 pigs at slaughter belonging to 67 batches were tested for y. enterocolitica and y. pseudotuberculosis in tonsils. diaphragm muscle samples were tested for antibodies against yersinia by a commercially available elisa test. y. enterocolitica 4/o:3 was detected in 55/201 pig tons ... | 2016 | 27500659 |
| characterization of european yersinia enterocolitica 1a strains using restriction fragment length polymorphism and multilocus sequence analysis. | yersinia enterocolitica is currently divided into two subspecies: subsp. enterocolitica including highly pathogenic strains of biotype 1b and subsp. palearctica including nonpathogenic strains of biotype 1a and moderately pathogenic strains of biotypes 2-5. in this work, we characterized 162 y. enterocolitica strains of biotype 1a and 50 strains of biotypes 2-4 isolated from human, animal and food samples by restriction fragment length polymorphism using the hindiii restriction enzyme. phylogene ... | 2016 | 27473388 |
| [detection of yersinia enterocolitica bacteriophage phiye-f10 lysis spectrum and analysis of the relationship between lysis ability and virulence gene of yersinia enterocolitica]. | to determine the lysis spectrum of yersinia enterocolitica bacteriophage phiye-f10 and to analyze the relationship between the lysis ability of phiye-f10 and the virulence gene of yersinia enterocolitica. to observe the lysis ability of the phage phiye-f10 to the different yersinia strains with the double-layer technique. the strains used in this study including 213 of yersinia enterocolitica and 36 of yersinia pseudotuberculosis and 1 of yersinia pestis. the virulence genes of these yersinia en ... | 2016 | 27396162 |
| the rna chaperone hfq is essential for virulence and modulates the expression of four adhesins in yersinia enterocolitica. | in enterobacteriaceae, the rna chaperone hfq mediates the interaction of small rnas with target mrnas, thereby modulating transcript stability and translation. this post-transcriptional control helps bacteria adapt quickly to changing environmental conditions. our previous mutational analysis showed that hfq is involved in metabolism and stress survival in the enteropathogen yersinia enterocolitica. in this study we demonstrate that hfq is essential for virulence in mice and influences productio ... | 2016 | 27387855 |
| yersinia virulence factors - a sophisticated arsenal for combating host defences. | the human pathogens yersinia pseudotuberculosis and yersinia enterocolitica cause enterocolitis, while yersinia pestis is responsible for pneumonic, bubonic, and septicaemic plague. all three share an infection strategy that relies on a virulence factor arsenal to enable them to enter, adhere to, and colonise the host while evading host defences to avoid untimely clearance. their arsenal includes a number of adhesins that allow the invading pathogens to establish a foothold in the host and to ad ... | 2016 | 27347390 |
| yersinia enterocolitica-specific infection by bacteriophages tg1 and ϕr1-rt is dependent on temperature-regulated expression of the phage host receptor ompf. | bacteriophages present huge potential both as a resource for developing novel tools for bacterial diagnostics and for use in phage therapy. this potential is also valid for bacteriophages specific for yersinia enterocolitica to increase our knowledge of y. enterocolitica-specific phages, we characterized two novel yersiniophages. the genomes of the bacteriophages vb_yenm_tg1 (tg1) and vb_yenm_ϕr1-rt (ϕr1-rt), isolated from pig manure in canada and from sewage in finland, consist of linear double ... | 2016 | 27342557 |
| all yersinia enterocolitica are pathogenic: virulence of phylogroup 1 y. enterocolitica in a galleria mellonella infection model. | yersinia enterocolitica is a zoonotic pathogen and a common cause of gastroenteritis in humans. the species is composed of six diverse phylogroups, of which strains of phylogroup 1 are considered non-pathogenic to mammals due to the lack of the major virulence plasmid pyv, and their lack of virulence in a mouse infection model. in the present report we present data examining the pathogenicity of strains of y. enterocolitica across all six phylogroups in a galleria mellonellla model. we have demo ... | 2016 | 27221796 |
| characterization of yersinia enterocolitica strains potentially virulent for humans and animals in river water. | the aim of this study was to isolate and identify potentially pathogenic strains of yersinia enterocolitica in water samples collected from the upstream section of the drwęca river in poland. | 2016 | 27203356 |
| four sporadic pediatric cases of yersinia enterocolitica o:8 infection in a rural area of japan. | in the spring of 2015, we experienced a cluster of 4 sporadic cases of yersiniosis in children in nagano prefecture, a rural area of japan. two patients developed appendicitis-like episodes; one had acute gastroenteritis, and the other had bacteremia associated with liver abscess. the causative agent of these infections was yersinia enterocolitica serogroup o:8. none of the patients had an underlying illness, and all have recovered completely. the patients were neither socially nor geographicall ... | 2017 | 27169952 |
| rna-sequencing reveals the progression of phage-host interactions between φr1-37 and yersinia enterocolitica. | despite the expanding interest in bacterial viruses (bacteriophages), insights into the intracellular development of bacteriophage and its impact on bacterial physiology are still scarce. here we investigate during lytic infection the whole-genome transcription of the giant phage vb_yecm_φr1-37 (φr1-37) and its host, the gastroenteritis causing bacterium yersinia enterocolitica. rna sequencing reveals that the gene expression of φr1-37 does not follow a pattern typical observed in other lytic ba ... | 2016 | 27110815 |
| mononuclear phagocytes contribute to intestinal invasion and dissemination of yersinia enterocolitica. | enteropathogenic yersinia enterocolitica (ye) enters the host via contaminated food. after colonisation of the small intestine ye invades the peyer's patches (pps) via m cells and disseminates to the mesenteric lymph nodes (mlns), spleen and liver. whether ye uses other invasion routes and which pathogenicity factors are required remains elusive. oral infection of lymphotoxin-β-receptor deficient mice lacking pps and mlns with ye revealed similar bacterial load in the spleen 1h post infection as ... | 2016 | 27107739 |
| monitoring of yersinia enterocolitica strains from free-living animals using different methods. | the aim of the study was to monitor y. enterocolitica strains from free-living animals captured during 2011-2014 hunting seasons in poland using warm (itc) and cold (psb) enrichment and molecular examination. over 1600 samples have been cultured. after itc/psb enrichment 237 strains presenting features characteristic for y. enterocolitica were isolated. molecular examination using multiplex pcr revealed 140 isolates from psb and 78 from itc. the concentration of pathogenic yersinia in asymptomat ... | 2016 | 27096808 |
| the mechanisms of complement activation in normal bovine serum and normal horse serum against yersinia enterocolitica o:9 strains with different outer membrane proteins content. | yersinia enterocolitica is a common zoonotic pathogen and facultative intracellular bacterium which can survive within blood cells. cattle and horses are considered a reservoir of y. enterocolitica which often causes several serious syndromes associated with yersiniosis such as abortions, premature births or infertility. the aim of our investigation was to determine the vitality of y. enterocolitica o:9 strains (ye9) in bovine and horse sera (nbs and nhrs) and explain the role of outer membrane ... | 2016 | 27096793 |
| prevalence of pathogenic yersinia enterocolitica in finnish slaughter pigs. | the prevalence of human pathogenic yersinia enterocolitica was determined in tonsil and intestinal content samples from 388 healthy fattening pigs at the four biggest finnish slaughterhouses. these slaughterhouses process 73% of pigs in finland. tonsil samples were tested by pcr targeted for yada, and intestinal samples were cultured. all pathogenic y. enterocolitica isolates represented bioserotype 4/o:3. the prevalence of y. enterocolitica in tonsil samples was 60% (95% confidence limit, 55.4 ... | 2016 | 27052875 |
| [two outbreaks of yersinia enterocolitica o:8 infections in tokyo and the characterization of isolates]. | although the number of outbreaks caused by yersinia enterocolitica has been very small in japan, 4 outbreaks were occurred during the 2 years between 2012 and 2013. we describe herein 2 outbreaks which were examined in tokyo in the present study. outbreak 1: a total of 39 people (37 high school students and 2 staff) stayed at a hotel in mountain area in japan had experienced abdominal pain, diarrhea and fever in august, 2012. the y. enterocolitica serogroup o:8 was isolated from 18 (64.3%) out o ... | 2016 | 27032176 |
| large diversity of porcine yersinia enterocolitica 4/o:3 in eight european countries assessed by multiple-locus variable-number tandem-repeat analysis. | a total of 253 multiple-locus variable-number tandem-repeat analysis (mlva) types among 634 isolates were discovered while studying the genetic diversity of porcine yersinia enterocolitica 4/o:3 isolates from eight different european countries. six variable-number tandem-repeat (vntr) loci v2a, v4, v5, v6, v7, and v9 were used to study the isolates from 82 farms in belgium (n = 93, 7 farms), england (n = 41, 8 farms), estonia (n = 106, 12 farms), finland (n = 70, 13 farms), italy (n = 111, 20 fa ... | 2016 | 26990517 |
| yersiniosis in france: overview and potential sources of infection. | the aim of this study was to exploit the extensive database on strains of yersinia collected over more than 50 years in france in order to gain an overview of yersiniosis and potential sources of contamination in this country. | 2016 | 26987478 |
| new pathogen-specific immunopet/mr tracer for molecular imaging of a systemic bacterial infection. | the specific and rapid detection of enterobacteriaceae, the most frequent cause of gram-negative bacterial infections in humans, remains a major challenge. we developed a non-invasive method to rapidly detect systemic yersinia enterocolitica infections using immunopet (antibody-targeted positron emission tomography) with [64cu]nodaga-labeled yersinia-specific polyclonal antibodies targeting the outer membrane protein yada. in contrast to the tracer [18f]fdg, [64cu]nodaga-yada uptake co-localized ... | 2016 | 26934329 |
| 'add, stir and reduce': yersinia spp. as model bacteria for pathogen evolution. | pathogenic species in the yersinia genus have historically been targets for research aimed at understanding how bacteria evolve into mammalian pathogens. the advent of large-scale population genomic studies has greatly accelerated the progress in this field, and yersinia pestis, yersinia pseudotuberculosis and yersinia enterocolitica have once again acted as model organisms to help shape our understanding of the evolutionary processes involved in pathogenesis. in this review, we highlight the ge ... | 2016 | 26876035 |
| analysis of differentially expressed proteins in yersinia enterocolitica-infected hela cells. | yersinia enterocolitica is a facultative intracellular pathogen and a causative agent of yersiniosis, which can be contracted by ingestion of contaminated food. yersinia secretes virulence factors to subvert critical pathways in the host cell. in this study we utilized shotgun label-free proteomics to study differential protein expression in epithelial cells infected with y.enterocolitica. we identified a total of 551 proteins, amongst which 42 were downregulated (including prostaglandin e synth ... | 2016 | 26854600 |
| [the effect of waal ligase genes deletion on motility and stress adaptation reactions of yersinia enterocolitica 6471/76]. | the aim of current study was to estimate the influence of waal(os) and waal(ps) genes deletion on lipopolysaccharide (lps) synthesis, bacterial motility and stress resistance of bacteria yersinia enterocolitica 6471/76. single and double waal mutants were created by replacing the wild-type alleles in bacterial chromosome for mutant ones. the phenotypes of mutants were visualized by doc-page gels stained with silver and immunoblot with specific to o-polysaccharide and outer core monoclonal antibo ... | 2016 | 26841489 |
| yersinia spp. identification using copy diversity in the chromosomal 16s rrna gene sequence. | api 20e strip test, the standard for enterobacteriaceae identification, is not sufficient to discriminate some yersinia species for some unstable biochemical reactions and the same biochemical profile presented in some species, e.g. yersinia ferderiksenii and yersinia intermedia, which need a variety of molecular biology methods as auxiliaries for identification. the 16s rrna gene is considered a valuable tool for assigning bacterial strains to species. however, the resolution of the 16s rrna ge ... | 2016 | 26808495 |
| systemic activation of tlr3-dependent trif signaling confers host defense against gram-negative bacteria in the intestine. | recognition of gram-negative bacteria by toll-like receptor (tlr)4 induces myd88 and trif mediated responses. we have shown that trif-dependent responses play an important role in intestinal defense against gram-negative enteropathogens. in the current study, we examined underlying mechanisms of how systemic trif activation enhances intestinal immune defense against gram-negative bacteria. first we confirmed that the protective effect of poly i:c against enteric infection of mice with yersinia e ... | 2015 | 26793623 |
| ileocecal resection for massive rectal bleeding due to yersinia enterocolitica: a case report and review of the literature. | massive gastrointestinal bleeding is an emergency that can sometimes require immediate surgery. we report the first case, to the best of our knowledge, of massive rectal bleeding due to yersinia enterocolitica, requiring ileocecal resection. | 2016 | 26781434 |
| genetic and metabolic signals during acute enteric bacterial infection alter the microbiota and drive progression to chronic inflammatory disease. | chronic inflammatory disorders are thought to arise due to an interplay between predisposing host genetics and environmental factors. for example, the onset of inflammatory bowel disease is associated with enteric proteobacterial infection, yet the mechanistic basis for this association is unclear. we have shown previously that genetic defiency in tlr1 promotes acute enteric infection by the proteobacteria yersinia enterocolitica. examining that model further, we uncovered an altered cellular im ... | 2016 | 26764594 |
| acute gastroenteritis leaves a lasting impression. | host immunity shapes intestinal microbiota composition, influencing health and disease. in this issue of cell host & microbe,kamdar et al. (2016) demonstrate that an aberrant acute inflammatory response to yersinia enterocolitica infection leads to long-lasting shifts in commensal communities and renders the host susceptible to chronic inflammation despite pathogen clearance. | 2016 | 26764589 |
| chicoric acid binds to two sites and decreases the activity of the yoph bacterial virulence factor. | chicoric acid (ca) is a phenolic compound present in dietary supplements with a large spectrum of biological properties reported ranging from antioxidant, to antiviral, to immunostimulatory properties. due to the fact that chicoric acid promotes phagocytic activity and was reported as an allosteric inhibitor of the ptp1b phosphatase, we examined the effect of ca on yoph phosphatase from pathogenic bacteria, which block phagocytic processes of a host cell. we performed computational studies of ch ... | 2016 | 26735581 |
| recombinant bivalent fusion protein rve induces cd4+ and cd8+ t-cell mediated memory immune response for protection against yersinia enterocolitica infection. | studies investigating the correlates of immune protection against yersinia infection have established that both humoral and cell mediated immune responses are required for the comprehensive protection. in our previous study, we established that the bivalent fusion protein (rve) comprising immunologically active regions of y. pestis lcrv (100-270 aa) and yope (50-213 aa) proteins conferred complete passive and active protection against lethal y. enterocolitica 8081 challenge. in the present study ... | 2015 | 26733956 |
| hypoxia decreases invasin-mediated yersinia enterocolitica internalization into caco-2 cells. | yersinia enterocolitica is a major cause of human yersiniosis, with enterocolitis being a typical manifestation. these bacteria can cross the intestinal mucosa, and invade eukaryotic cells by binding to host β1 integrins, a process mediated by the bacterial effector protein invasin. this study examines the role of hypoxia on the internalization of y. enterocolitica into intestinal epithelial cells, since the gastrointestinal tract has been shown to be physiologically deficient in oxygen levels ( ... | 2016 | 26731748 |
| role of β1 integrins and bacterial adhesins for yop injection into leukocytes in yersinia enterocolitica systemic mouse infection. | injection of yersinia outer proteins (yops) into host cells by a type iii secretion system is an important immune evasion mechanism of yersinia enterocolitica (ye). in this process ye invasin (inv) binds directly while yersinia adhesin a (yada) binds indirectly via extracellular matrix (ecm) proteins to β1 integrins on host cells. although leukocytes turned out to be an important target of yop injection by ye, it was unclear which ye adhesins and which leukocyte receptors are required for yop in ... | 2016 | 26718660 |
| pathogenic gut flora in patients with chronic heart failure. | the goal of this study was to measure the presence of pathogenic gut flora and intestinal permeability (ip) and their correlations with disease severity, venous blood congestion, and inflammation in patients with chronic heart failure (chf). | 2016 | 26682791 |
| multispecies epidemiologic surveillance study after an outbreak of yersiniosis at an african green monkey research facility. | after an outbreak of yersinia enterocolitica at a nhp research facility, we performed a multispecies investigation of the prevalence of yersinia spp. in various mammals that resided or foraged on the grounds of the facility, to better understand the epizootiology of yersiniosis. blood samples and fecal and rectal swabs were obtained from 105 captive african green monkeys (agm), 12 feral cats, 2 dogs, 20 mice, 12 rats, and 3 mongooses. total dna extracted from swab suspensions served as template ... | 2015 | 26678370 |
| impact of ompr on the membrane proteome of yersinia enterocolitica in different environments: repression of major adhesin yada and heme receptor hemr. | enteropathogenic yersinia enterocolitica is able to grow within or outside the mammalian host. previous transcriptomic studies have indicated that the regulator ompr plays a role in the expression of hundreds of genes in enterobacteria. here, we have examined the impact of ompr on the production of y. enterocolitica membrane proteins upon changes in temperature, osmolarity and ph. proteomic analysis indicated that the loss of ompr affects the production of 120 proteins, a third of which are invo ... | 2016 | 26627632 |
| detection and characterisation of yersinia enterocolitica strains in cold-stored carcasses of large game animals in poland. | yersinia enterocolitica is an important foodborne pathogen. the aim of the present study was to identify the bioserotypes and virulence markers of y.enterocolitica strains isolated from three different anatomical regions of cold-stored carcasses of large game animals intended for human consumption. y.enterocolitica strains were found in 12/20 (60%) of the roe deer carcasses examined, 7/16 (43.8%) of red deer carcasses and 11/20 (55%) of wild boar carcasses. of the 52 y.enterocolitica strains, 19 ... | 2016 | 26626093 |
| yersinia enterocolitica affects intestinal barrier function in the colon. | infection with yersinia enterocolitica causes acute diarrhea in early childhood. a mouse infection model presents new findings on pathological mechanisms in the colon. symptoms involve diarrhea with watery feces and weight loss that have their functional correlates in decreased transepithelial electrical resistance and increased fluorescein permeability. y. enterocolitica was present within the murine mucosa of both ileum and colon. here, the bacterial insult was of focal nature and led to chang ... | 2016 | 26621910 |
| a rapid method for the nonselective enumeration of yersinia enterocolitica, a foodborne pathogen associated with pork. | an impedance method was developed as a rapid, convenient method to enumerate pure culture of yersinia enterocolitica. cultures were incubated in trypticase soy broth (tsb) at 30°c. the bactrac™ 4000 microorganism growth analyser was used to detect impedance change of tsb representing bacteria cell numbers in the samples. good correlations with standard plate counts were obtained (r(2)>0.95). this method is also reliable to enumerate y. enterocolitica growing in biofilms attached to stainless ste ... | 2016 | 26613189 |
| comparative genomic hybridization analysis of yersinia enterocolitica and yersinia pseudotuberculosis identifies genetic traits to elucidate their different ecologies. | enteropathogenic yersinia enterocolitica and yersinia pseudotuberculosis are both etiological agents for intestinal infection known as yersiniosis, but their epidemiology and ecology bear many differences. swine are the only known reservoir for y. enterocolitica 4/o:3 strains, which are the most common cause of human disease, while y. pseudotuberculosis has been isolated from a variety of sources, including vegetables and wild animals. infections caused by y. enterocolitica mainly originate from ... | 2015 | 26605338 |
| ex vivo perfusion of the isolated rat small intestine as a novel model of salmonella enteritis. | using an ex vivo perfused rat small intestinal model, we examined pathological changes to the tissue, inflammation induction, as well as dynamic changes to smooth muscle activity, metabolic competence, and luminal fluid accumulation during short-term infection with the enteropathogenic bacteria salmonella enterica serovar typhimurium and yersinia enterocolitica. although few effects were seen upon yersinia infection, this system accurately modeled key aspects associated with salmonella enteritis ... | 2016 | 26564721 |
| mesenteric lymphadenitis caused by yersinia enterocolitica. | yersiniosis is an acute or chronic, zoonotic disease caused by infection of gram-negative rods yersinia enterocolitica. it can be transmitted by the consumption of originally contaminated food products (pork, unpasteurized milk) or secondarily contaminated with animal or vegetable products. the clinical picture of infection may have a variable course is related to the age and physical condition of the patient, or pathogenic properties of microorganisms. infection caused by y. enterocolitica can ... | 2015 | 26557944 |
| another mistaken case of appendicitis. | in 2010, when i was 17, i presented to hospital with progressive discomfort and pain in the right iliac fossa when eating and moving, associated with mild fever and diarrhoea. appendicitis was suspected but immediate surgery was deferred, as the inflammatory markers did not adequately support the clinical diagnosis of appendicitis. further tests, including mri, were then undertaken. the mri showed evidence of terminal ileitis and a normal appendix. crohn's disease was considered as part of the d ... | 2015 | 26504093 |
| analysis of yersinia enterocolitica effector translocation into host cells using beta-lactamase effector fusions. | many gram-negative bacteria including pathogenic yersinia spp. employ type iii secretion systems to translocate effector proteins into eukaryotic target cells. inside the host cell the effector proteins manipulate cellular functions to the benefit of the bacteria. to better understand the control of type iii secretion during host cell interaction, sensitive and accurate assays to measure translocation are required. we here describe the application of an assay based on the fusion of a yersinia en ... | 2015 | 26484613 |
| prevalence, antimicrobial resistance and genetic diversity of yersinia enterocolitica isolated from retail frozen foods in china. | in this study, our aim was to estimate the extent of yersinia enterocolitica contamination in frozen foods in china and determine the bioserotype, virulotype, antimicrobial resistance, and enterobacterial repetitive intergenic consensus-pcr (eric-pcr) genotyping profiles of recovered y. enterocolitica isolates. out of 455 samples collected between july 2011 and may 2014, 56 (12.3%) tested positive for y. enterocolitica. the 70 isolated strains were grouped into five clusters and one singleton ba ... | 2015 | 26472688 |
| yersinia enterocolitica-mediated degradation of neutrophil extracellular traps (nets). | neutrophil extracellular trap (net) formation is described as a tool of the innate host defence to fight against invading pathogens. fibre-like dna structures associated with proteins such as histones, cell-specific enzymes and antimicrobial peptides are released, thereby entrapping invading pathogens. it has been reported that several bacteria are able to degrade nets by nucleases and thus evade the net-mediated entrapment. here we studied the ability of three different yersinia serotypes to in ... | 2015 | 26459885 |
| homology analysis and cross-immunogenicity of ompa from pathogenic yersinia enterocolitica, yersinia pseudotuberculosis and yersinia pestis. | the outer membrane protein a (ompa) is one of the intra-species conserved proteins with immunogenicity widely found in the family of enterobacteriaceae. here we first confirmed ompa is conserved in the three pathogenic yersinia: yersinia pestis, yersinia pseudotuberculosis and pathogenic yersinia enterocolitica, with high homology at the nucleotide level and at the amino acid sequence level. the identity of ompa sequences for 262 y. pestis strains, 134 y. pseudotuberculosis strains and 219 patho ... | 2015 | 26435220 |
| directional gene flow and ecological separation in yersinia enterocolitica. | yersinia enterocolitica is a common cause of food-borne gastroenteritis worldwide. recent work defining the phylogeny of the genus yersinia subdivided y. enterocolitica into six distinct phylogroups. here, we provide detailed analyses of the evolutionary processes leading to the emergence of these phylogroups. the dominant phylogroups isolated from human infections, pg3-5, show very little diversity at the sequence level, but do present marked patterns of gain and loss of functions, including th ... | 2015 | 28348815 |
| characterisation of yersinia secretion apparatus--pathogenicity island (ysa-pi) of yersinia enterocolitica 1b/o8 in poland: an idle ysa is a specific hallmark of the epidemic sensu stricto strain. | yersinia secretion apparatus (ysa), the chromosomal type three secretion system (t3ss) is considered to contribute to virulence of high-pathogenicity yersina enterocolitica biovar 1b. dna-sequence of ysa pathogenicity island was determined for clinical isolate dm0110 of y enterocolitica 1b/08 with origin in poland. we found a premature stop-codon in the regulatory gene ysrr (mutation at position 269). altered ysrr was detected in all tested 78 isolates of y enterocolitica 1b/o8 collected from cl ... | 2015 | 26373178 |
| evaluation of the pathogenic potential, antimicrobial susceptibility, and genomic relations of yersinia enterocolitica strains from food and human origin. | yersinia enterocolitica is a food-borne pathogen that causes gastroenteritis with occasional postinfection sequels. this study was aimed to determinate the pathogenic potential, antimicrobial susceptibility, and genomic relationships of y. enterocolitica strains of different bioserotypes (b/o) isolated from foods and human samples in san luis, argentina. strains obtained by culture were bioserotyped and characterized by phenotypic and genotypic virulence markers, antimicrobial susceptibility, an ... | 2015 | 26370735 |
| molecular docking based screening of neem-derived compounds with the ns1 protein of influenza virus. | different strains of influenza virus are affecting a large number of people worldwide to combat with influenza virus destruction, numerous synthetic antiviral medicines are available for influenza virus in the market. but still there was a need for the development of drug which will target all the strains of influenza virus. for this purpose conserved residues within the influenza virus ns1 protein have been found by aligning all the available sequences of existing strains from the national cent ... | 2015 | 26339153 |
| detection of yersinia enterocolitica in milk powders by cross-priming amplification combined with immunoblotting analysis. | yersinia enterocolitica (y. enterocolitica) is frequently isolated from a wide variety of foods and can cause human yersiniosis. biochemical and culture-based assays are common detection methods, but require a long incubation time and easily misidentify y. enterocolitica as other non-pathogenic yersinia species. alternatively, cross-priming amplification (cpa) under isothermal conditions combined with immunoblotting analysis enables a more sensitive detection in a relatively short time period. a ... | 2015 | 26253307 |
| role of electrostatic interactions for ligand recognition and specificity of peptide transporters. | peptide transporters are membrane proteins that mediate the cellular uptake of di- and tripeptides, and of peptidomimetic drugs such as β-lactam antibiotics, antiviral drugs and antineoplastic agents. in spite of their high physiological and pharmaceutical importance, the molecular recognition by these transporters of the amino acid side chains of short peptides and thus the mechanisms for substrate binding and specificity are far from being understood. | 2015 | 26246134 |
| analysis of iron acquisition and storage-related genes in clinical and non-clinical strains of yersinia enterocolitica biovar 1a. | possession of mechanisms for iron acquisition and its storage enhances the ability of the bacteria to survive in the iron-limiting environment of the host. in this study, 81 strains of yersinia enterocolitica biovar 1a isolated from various clinical (n = 51) and non-clinical (n = 30) sources were investigated for the presence of the genes related to iron acquisition and storage. important genes which were present in more than 85% of the strains included hasa, foxa, bfr, bfd, ftna, and hmst as we ... | 2015 | 26223204 |
| interaction of human mannose-binding lectin (mbl) with yersinia enterocolitica lipopolysaccharide. | the lipopolysaccharide (lps) is involved in the interaction between gram-negative pathogenic bacteria and host. mannose-binding lectin (mbl), complement-activating soluble pattern-recognition receptor targets microbial glycoconjugates, including lps. we studied its interactions with a set of yersinia enterocolitica o:3 lps mutants. the wild-type strain lps consists of lipid a (la) substituted with an inner core oligosaccharide (ic) which in turn is substituted either with the o-specific polysacc ... | 2015 | 26188838 |
| biochemical studies on wbca, a sugar epimerase from yersinia enterocolitica. | yersinia enterocolitica is a gram-negative bacterium that causes yersiniosis, a zoonotic disease affecting the gastrointestinal tract of humans, cattle, and pigs, among others. the lipopolysaccharide of y. enterocolitica o:8 contains an unusual sugar, 6-deoxy-d-gulose, which requires four enzymes for its biosynthesis. here, we describe a combined structural and functional investigation of wbca, which catalyzes the third step in the pathway, namely an epimerization about the c-3' carbon of a cdp- ... | 2015 | 26174084 |
| yersinia enterocolitica strains isolated from beavers (castor fiber). | pseudocloacal swabs and palatine tonsils from beavers have been examined for the yersinia enterocolitica presence. thirty-six samples from 24 beavers were collected and subjected to bacteriological examinations including sero- and biotypisation. amplicons confirmed by pcr as y. enterocolitica were sequenced. positive samples originated from 4 out of the 24 beavers (16.7 %) and all the strains belonged to biotype 1a. the study suggested that y. enterocolitica could be isolated from beavers, which ... | 2015 | 26172198 |
| innate immune system favors emergency monopoiesis at the expense of dc-differentiation to control systemic bacterial infection in mice. | dcs are professional apcs playing a crucial role in the initiation of t-cell responses to combat infection. however, systemic bacterial infection with various pathogens leads to dc-depletion in humans and mice. the mechanisms of pathogen-induced dc-depletion remain poorly understood. previously, we showed that mice infected with yersinia enterocolitica (ye) had impaired de novo dc-development, one reason for dc-depletion. here, we extend these studies to gain insight into the molecular mechanism ... | 2015 | 26138432 |
| genetic diversity among yersinia enterocolitica isolated from chicken and fish in and around coimbatore city, india. | yersinia enterocolitica is rapidly emerging worldwide as an enteric pathogen and has become a major cause of diarrhea even in developed countries. the aim of this study was to characterize and genetic diversity analysis among y. enterocolitica strains isolated from fish and chicken sources. | 2014 | 26110155 |
| detection of pathogenic yersinia enterocolitica in slaughtered pigs by cultural methods and real-time polymerase chain reaction. | healthy pigs carrying pathogenic to human yersinia enterocolitica strains are the main source of entry into slaughterhouse, where cross-contamination of carcasses can happen. the aim of this work was to determine y. enterocolitica prevalence in slaughtered pigs, investigating the presence of carriers in relation to carcass contamination. a total of 132 pig samples (tonsils, mesenteric lymph nodes, colon content, carcass surface) were collected from 4 sardinian slaughterhouses. all the samples we ... | 2015 | 27800392 |
| ecology and geographic distribution of yersinia enterocolitica among livestock and wildlife in china. | the results in this study show the prevalence of yersinia enterocolitica varies in different animal species and regions of china. the highest prevalence is among pigs (12.91%), followed by dogs (9.80%), ochotona curzoniae (plateau pica) (6.76%), chickens (4.50%), rodents (3.40%), cattle (2.78%) and sheep (0.89%). pathogenic isolates comprised the majority of the y. enterocolitica recovered from pigs (73.50%) and dogs (59.44%); whereas the nonpathogenic y. enterocolitica made up most of poultry a ... | 2015 | 25987302 |
| genotypic and phenotypic virulence characteristics and antimicrobial resistance of yersinia spp. isolated from meat and milk products. | a total of 300 food samples including 180 milk and 120 meat products have been examined for the presence of yersinia spp. using the iso 10273 and the cold enrichment method. the overall prevalence of yersinia spp. was 84 (28%). yersinia enterocolitica was isolated from 18 (6%) of the 300 samples. the other yersinia species were detected in the samples yersinia rohdei 15 (5%), yersinia intermedia 14 (4.7%), yersinia pseudotuberculosis 12 (4%), yersinia ruckeri 12 (4%), yersinia mollaretii 5 (1.7% ... | 2015 | 25969137 |
| the descriptive epidemiology of yersiniosis: a multistate study, 2005-2011. | yersiniosis, a foodborne infection of zoonotic origin caused by the bacteria yersinia enterocolitica and yersinia pseudotuberculosis, is a reportable disease in 38 states. both sporadic and foodborne outbreaks of yersiniosis have been reported in the u.s., with annual occurrence of an estimated 98,000 episodes of illness, 533 hospitalizations, and 29 deaths. we analyzed surveillance data from nine non-foodnet-participating u.s. states during the period 2005-2011 to describe the epidemiology of t ... | 2017 | 25931631 |
| proteomic analysis of yersinia enterocolitica biovar 1a under iron-rich and iron-poor conditions indicate existence of efficiently regulated mechanisms of iron homeostasis. | the pathogenicity of yersinia enterocolitica biovar 1a strains is controversial as these lack most of the known virulence factors. acquisition of iron and presence of well-regulated iron homeostasis in bacteria represents an important virulence trait. differential abundance of proteins was examined under iron-rich and iron-poor conditions in a clinical y. enterocolitica biovar 1a strain ip27407. whole cell protein profiles were analysed by 2d gel electrophoresis (2d-ge). following statistical an ... | 2015 | 25913300 |
| isolation of pathogenic yersinia enterocolitica strains from different sources in izmir region, turkey. | yersinia enterocolitica is a foodborne pathogen that is very rarely encountered in turkey. in this work, several human, porcine, and environmental samples collected from izmir region in turkey were examined for the presence of y. enterocolitica using different cultivation and enrichment methods. a total of nine pathogenic y. enterocolitica strains were isolated; five strains from pig stool and manure samples and four strains from waste water samples. on the other hand, no y. enterocolitica was i ... | 2015 | 25912845 |
| expression of the acrab components of the acrab-tolc multidrug efflux pump of yersinia enterocolitica is subject to dual regulation by ompr. | ompr is a transcriptional regulator implicated in the control of various cellular processes and functions in enterobacteriaceae. this study was undertaken to identify genes comprising the ompr regulon in the human gastrointestinal pathogen yersinia enterocolitica. derivatives of an ompr-negative strain with random transposon insertions creating transcriptional fusions with the reporter gene lacz were isolated. these were supplied with the wild-type ompr allele in trans and then screened for ompr ... | 2015 | 25893523 |
| assessment of risk factors for a high within-batch prevalence of yersinia enterocolitica in pigs based on microbiological analysis at slaughter. | the purpose of the current study was to find farm-level factors influencing the bacteriological prevalence of yersinia enterocolitica in pigs at time of slaughter. on 100 farms, data concerning a broad range of farm aspects (e.g., management and housing system, biosecurity, and hygiene measurements) were collected using a face-to-face questionnaire. at the slaughterhouse, tonsils of on average 70 slaughter pigs per batch were sampled to determine the infection status of pigs. after univariable m ... | 2015 | 25884541 |
| contamination of freshly slaughtered pig carcasses with enteropathogenic yersinia spp.: distribution, quantification and identification of risk factors. | a cross-sectional survey was undertaken to determine the overall prevalence of enteropathogenic yersinia spp. in the tonsils, feces and on carcasses of pigs at slaughter. moreover, factors associated with yersinia contamination of freshly eviscerated pig carcasses were studied. yersinia enterocolitica serotype o:3 was isolated from the tonsils and feces of 55.3% and 25.6% of pigs, and y. pseudotuberculosis from 1.4% and 0.6%, respectively. the pathogens were also recovered from 39.7% of carcass ... | 2015 | 25835198 |
| investigation of an unusual increase in human yersinioses in creuse, france. | to investigate an unusual cluster of y. enterocolitica 4/o:3/viii human infections that occurred in creuse (france) during the summer 2008, and to perform retrospective and prospective analyses of yersiniosis cases to get a better view of the general trend. | 2015 | 25800947 |
| novel r pipeline for analyzing biolog phenotypic microarray data. | data produced by biolog phenotype microarrays are longitudinal measurements of cells' respiration on distinct substrates. we introduce a three-step pipeline to analyze phenotypic microarray data with novel procedures for grouping, normalization and effect identification. grouping and normalization are standard problems in the analysis of phenotype microarrays defined as categorizing bacterial responses into active and non-active, and removing systematic errors from the experimental data, respect ... | 2015 | 25786143 |
| expression of the yersinia enterocolitica o:3 lps o-antigen and outer core gene clusters is rfah-dependent. | the antiterminator rfah is required for the expression of lps, capsule, haemolysin, exotoxin, haemin uptake receptor and f pilus. as these structures are critical for bacterial virulence, loss of rfah usually leads to attenuation. here, we inactivated the rfah gene of yersinia enterocolitica o:3 to study its role in this enteropathogen. rna sequencing of the wt and δrfah strain transcriptomes revealed that rfah acted as a highly specific regulator that enhanced the transcription of the operons i ... | 2015 | 25767108 |
| a fatal case of post-transfusion sepsis caused by yersinia enterocolitica after delivery. | | 2015 | 25761326 |
| co-expression of the c-terminal domain of yersinia enterocolitica invasin enhances the efficacy of classical swine-fever-vectored vaccine based on human adenovirus. | the use of adenovirus vector-based vaccines is a promising approach for generating antigen-specific immune responses. improving vaccine potency is necessary in other approaches to address their inadequate protection for the majority of infectious diseases. this study is the first to reconstruct a recombinant replication-defective human adenovirus co-expressing e2 and invasin c-terminal (invc) glycoproteins (rad-e2-invc). rad-e2-invc with 2 x 10(6) tcid50 was intramuscularly administered two time ... | 2015 | 25740144 |
| a case of septic arthritis of the shoulder due to yersinia enterocolitica with review of the literature. | yersinia enterocolitica infection rarely can cause extra-intestinal infections. we present a case of septic arthritis of the shoulder due to this organism in an elderly man with liver and cardiac disease. we review previously published cases of y. enterocolitica septic arthritis, and discuss risk factors and management. | 2014 | 25734124 |
| yersinia enterocolitica exploits different pathways to accomplish adhesion and toxin injection into host cells. | the current paradigm suggests that yersinia enterocolitica (ye) adheres to host cells via the outer membrane proteins yersinia adhesin a (yada) or invasin (inv) to facilitate injection of yops by the type iii secretion system. in this process inv binds directly to β1 integrins of host cells while yada may bind indirectly via extracellular matrix proteins to β1 integrins. here we challenged this paradigm and investigated the requirements for yop injection. we demonstrate that inv- but not yada-me ... | 2015 | 25678064 |
| purification and characterization of an extracellular cold-adapted alkaline lipase produced by psychrotrophic bacterium yersinia enterocolitica strain km1. | an extracellular cold-adapted alkaline lipase from the psychrotrophic yersinia enterocolitica strain km1 was purified 26-fold to homogeneity. the enzyme was active over a broad range spanning 0-60 °c with an optimum activity at 37 °c, and it was found to be alkaline-preferring with an optimum activity at ph 9.0. the molecular weight was estimated to be 34.3 kda and monomeric. the lipase could be activated by ca(2+) and low concentration (10%) of ethanol, dimethyl sulphoxide, methanol, and aceton ... | 2015 | 25677080 |
| yersinia effector yopo uses actin as bait to phosphorylate proteins that regulate actin polymerization. | pathogenic yersinia species evade host immune systems through the injection of yersinia outer proteins (yops) into phagocytic cells. one yop, yopo, also known as ypka, induces actin-filament disruption, impairing phagocytosis. here we describe the x-ray structure of yersinia enterocolitica yopo in complex with actin, which reveals that yopo binds to an actin monomer in a manner that blocks polymerization yet allows the bound actin to interact with host actin-regulating proteins. silac-ms and bio ... | 2015 | 25664724 |
| genotypic diversity and pathogenic potential of yersinia enterocolitica biotype 2 strains isolated in brazil. | to investigate the pathogenic potential and genotypic diversity of yersinia enterocolitica biotype 2 strains isolated in brazil and to compare these strains with other y. enterocolitica biotypes using eric-pcr and pfge. | 2015 | 25639495 |
| virulence-associated gene pattern of porcine and human yersinia enterocolitica biotype 4 isolates. | yersinia enterocolitica 4/o:3 is the most important human pathogenic bioserotype in europe and the predominant pathogenic bioserotype in slaughter pigs. although many studies on the virulence of y. enterocolitica strains have showed a broad spectrum of detectable factors in pigs and humans, an analysis based on a strict comparative approach and serving to verify the virulence capability of porcine y. enterocolitica as a source for human yersiniosis is lacking. therefore, in the present study, st ... | 2015 | 25617775 |
| the first pathogenic yersinia enterocolitica bioserotype 4/o:3 strain isolated from a hunted wild boar (sus scrofa) in poland. | the objective of this study was to identify the bioserotypes and virulence markers of yersinia enterocolitica strains isolated from wild boars in poland. bacteriological examination of 302 rectal swabs from 151 wild boars resulted in the isolation of 40 y. enterocolitica strains. the majority of the examined strains (n = 30), belonged to bioserotype 1a/ni. the presence of individual y. enterocolitica strains belonging to bioserotypes 1b/ni (3), 1a/o:8 (2), 1a/o:27 (2), 2/ni (1), 2/o:9 (1) and 4/ ... | 2015 | 25608621 |
| yersinia adhesin a (yada)--beauty & beast. | the trimeric autotransporter adhesin yersinia adhesin a is the prototype of the type vc secretion systems. it is expressed by enteropathogenic yersinia enterocolitica and yersinia pseudotuberculosis strains, but not by yersinia pestis. a characteristic trait of yada is its modular composition and trimeric nature. yada consists of an n-terminal passenger domain which is exposed on the bacterial cell surface. the translocation of this passenger onto the surface is facilitated by a c-terminal β-bar ... | 2015 | 25604505 |
| yersiniabase: a genomic resource and analysis platform for comparative analysis of yersinia. | yersinia is a gram-negative bacteria that includes serious pathogens such as the yersinia pestis, which causes plague, yersinia pseudotuberculosis, yersinia enterocolitica. the remaining species are generally considered non-pathogenic to humans, although there is evidence that at least some of these species can cause occasional infections using distinct mechanisms from the more pathogenic species. with the advances in sequencing technologies, many genomes of yersinia have been sequenced. however ... | 2015 | 25591325 |
| bayesian modelling to identify the risk factors for yersinia enterocolitica contamination of pork carcasses and pluck sets in slaughterhouses. | the probability of contamination by pathogenic yersinia enterocolitica of carcasses and pluck sets at slaughterhouse was determined by means of a bayesian analysis. prior information of the prevalence of y. enterocolitica in faeces and the seroprevalence of yersinia in serum of pigs collected at farms were obtained from previous studies and introduced in the models as beta prior informative distributions. samples of intestinal content, tonsils, and swabs of carcasses and pluck set surfaces were ... | 2015 | 25555229 |
| yersinia enterocolitica septicemia after chitterling ingestion in a pediatric patient with iron overload disease: a case report. | yersinia enterocolitica is a gram-negative cocobacillus causing a range of illness from self-limited enteritis to invasive disease, including septicemia. it is a particularly virulent pathogen in patients with underlying hemoglobinopathies who are predisposed to iron overload. a substantial risk factor for disease in children and infants is exposure to the household preparation of chitterlings. early identification of these patients is critical in the pediatric intensive care unit as this cause ... | 2015 | 27335968 |
| a child with kawasaki disease and yersinia enterocolitica infection: a closer look at pathogenesis. | | 2015 | 27335962 |
| yersinia enterocolitica type iii secretion injectisomes form regularly spaced clusters, which incorporate new machines upon activation. | bacterial type iii secretion systems or injectisomes are multiprotein complexes directly transporting bacterial effector proteins into eukaryotic host cells. to investigate the distribution of injectisomes in the bacterium and the influence of activation of the system on that distribution, we combined in vivo fluorescent imaging and high-resolution in situ visualization of yersinia enterocolitica injectisomes by cryo-electron tomography. fluorescence microscopy showed the injectisomes as regular ... | 2015 | 25524451 |
| manipulation of pro-inflammatory cytokine production by the bacterial cell-penetrating effector protein yopm is independent of its interaction with host cell kinases rsk1 and prk2. | the effector protein yersinia outer protein m (yopm) of yersinia enterocolitica has previously been identified and characterized as the first bacterial cell-penetrating protein (cpp). we found that recombinant yopm (ryopm) enters different eukaryotic cell types and downregulates the expression of several pro-inflammatory cytokines (e.g., tumor necrosis factor-α [tnf-α]) after autonomous translocation. after infection with y. enterocolitica or transfection of host cells, yopm interacts with isofo ... | 2014 | 25513777 |
| phenotypic and genotypic analysis of bio-serotypes of yersinia enterocolitica from various sources in brazil. | yersinia enterocolitica is a well-known foodborne pathogen widely distributed in nature with high public health relevance, especially in europe. | 2014 | 25500651 |
| prevalence, characterization, and antimicrobial resistance of yersinia species and yersinia enterocolitica isolated from raw milk in farm bulk tanks. | the aims of this study were to investigate the prevalence and to characterize and determine the antibiotic resistance of yersinia spp. isolates from raw milk. from september 2008 to august 2010, 446 raw milk samples were obtained from farm bulk milk tanks in varamin, iran. yersinia spp. were detected in 29 (6.5%) samples, out of which 23 (79.3%), 5 (17.2%), and 1 (3.4%) were isolated from cow, sheep, and goat raw milk, respectively. the most common species isolated was yersinia enterocolitica (6 ... | 2015 | 25497824 |
| isolation and characterization of yersinia-specific bacteriophages from pig stools in finland. | bacteriophages infect bacteria, and they are present everywhere in the world including the intestinal tracts of animals. yersiniosis is a common foodborne infection caused by yersinia enterocolitica and yersinia pseudotuberculosis. as these bacteria are frequently isolated from pigs, we wanted to know whether yersinia-specific bacteriophages are also present in the pig stools and, if so, whether there is a positive or negative association between the prevalence of the yersinia phages and the pat ... | 2015 | 25495090 |