| foodborne bacterial pathogens in retail prepacked ready-to-eat mixed ingredient salads. | prepacked ready-to-eat mixed ingredient salads (rte salads) are readily available whole meals that include a variety of ingredients such as raw vegetables, cooked meat, and pasta. as part of a trend toward healthy convenience foods, rte salads have become an increasingly popular product among consumers. however, data on the incidence of foodborne pathogens in rte salads are scarce. in this study, the microbiological safety of 141 rte salads containing chicken, ham, or smoked salmon was investiga ... | 2016 | 27296602 |
| label-free gram-negative bacteria detection using bacteriophage-adhesin-coated long-period gratings. | this paper presents a novel application of a highly sensitive sensor based on long-period gratings (lpgs) coated with t4 bacteriophage adhesin for gram-negative bacteria detection. we show here, that the sensor evidently recognizes escherichia coli k-12 (pcm2560), whereas in the reference tests - elisa and biacore - the results are questionable. for lpgs sensor the resonant wavelength shift observed for e. coli k-12 was approximately half of that measured for e.coli b (positive control). the bia ... | 2016 | 27231592 |
| complete genome sequence and comparative genome analysis of a new special yersinia enterocolitica. | yersinia enterocolitica is the most diverse species among the yersinia genera and shows more polymorphism, especially for the non-pathogenic strains. individual non-pathogenic y. enterocolitica strains are wrongly identified because of atypical phenotypes. in this study, we isolated an unusual y. enterocolitica strain lc20 from rattus norvegicus. the strain did not utilize urea and could not be classified as the biotype. api 20e identified escherichia coli; however, it grew well at 25 °c, but e. ... | 2016 | 27129539 |
| microbiological quality of the water used in agriculture in sardinia. | the microbiological quality of the water used in irrigation is crucial for the safety of products, such as fruit and vegetables, especially when destined to be consumed raw. however, the microbiological quality of this water is not defined at a community regulatory level or at a national level. | 2016 | 27071326 |
| inclusion of an rgd motif alters invasin integrin-binding affinity and specificity. | invasin is a key adhesin displayed on the outer membrane of yersinia enterocolitica and y. pseudotuberculosis that mediates the initial stages of infection. invasin specifically targets microfold (m) cells in the small intestine by binding β1 integrins and is sufficient to trigger eukaryotic uptake of invasin-coated particles, including yersinia, escherichia coli, and latex beads. as a result, invasin has generated interest to mediate oral delivery of vaccines and other biologics. integrin bindi ... | 2016 | 27015583 |
| resistance to amoxicillin-clavulanate and its relation to virulence-related factors in yersinia enterocolitica biovar 1a. | recent studies have reported that the virulence factors (vfs) were detected more frequently in amoxicillin-clavulanate (amc) susceptible clinical isolates of escherichia coli. here, we have evaluated the relationship between vfs and amc-resistance phenotype in clinical isolates of y. enterocolitica biovar 1a. the presence/absence of vfs was compared with their minimum inhibitory concentrations for amc in strains of two serovars. we observed that the strains of the serovar o: 6, 30-6, 31 showed a ... | 2016 | 26776125 |
| antimicrobial activity of the essential oil from the leaves and seeds of coriandrum sativum toward food-borne pathogens. | the increasing incidence of drug-resistant pathogens and toxicity of existing antibacterial compounds has drawn attention toward the antimicrobial activity of natural products. the purpose of this study is to evaluate the antimicrobial activity of the essential oil of the leaves and seeds of coriandrum sativum. the five strains of bacteria comprising escherichia coli, staphylococcus aureus, yersinia enterocolitica, salmonella enterica and vibrio cholerae were used for the antibacterial tests. in ... | 2015 | 26684163 |
| molecular modeling and simulation studies of recombinant laccase from yersinia enterocolitica suggests significant role in the biotransformation of non-steroidal anti-inflammatory drugs. | the yack gene from yersinia enterocolitica strain 7, cloned in pet28a vector and expressed in escherichia coli bl21 (de3), showed laccase activity when oxidized with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (abts) and guaiacol. the recombinant laccase protein was purified and characterized biochemically with a molecular mass of ≈58 kda on sds-page and showed positive zymogram with abts. the protein was highly robust with optimum ph 9.0 and stable at 70 °c upto 12 h with residual act ... | 2016 | 26631965 |
| oral iron acutely elevates bacterial growth in human serum. | iron deficiency is the most common nutrient deficiency worldwide and routine supplementation is standard policy for pregnant mothers and children in most low-income countries. however, iron lies at the center of host-pathogen competition for nutritional resources and recent trials of iron administration in african and asian children have resulted in significant excesses of serious adverse events including hospitalizations and deaths. increased rates of malaria, respiratory infections, severe dia ... | 2015 | 26593732 |
| antibacterial activity, antioxidant effect and chemical composition of propolis from the región del maule, central chile. | propolis is commercialized in chile as an antimicrobial agent. it is obtained mainly from central and southern chile, but is used for the same purposes regardless of its origin. to compare the antimicrobial effect, the total phenolic (tp), the total flavonoid (tf) content and the phenolic composition, 19 samples were collected in the main production centers in the región del maule, chile. samples were extracted with meoh and assessed for antimicrobial activity against gram (+) and gram (-) bacte ... | 2015 | 26457694 |
| solid-state nmr study of the yada membrane-anchor domain in the bacterial outer membrane. | mas-nmr was used to study the structure and dynamics at ambient temperatures of the membrane-anchor domain of yada (yada-m) in a pellet of the outer membrane of e. coli in which it was expressed. yada is an adhesin from the pathogen yersinia enterocolitica that is involved in interactions with the host cell, and it is a model protein for studying the autotransport process. existing assignments were sucessfully transferred to a large part of the yada-m protein in the e. coli lipid environment by ... | 2015 | 26332158 |
| expression, purification and characterization of a functional, recombinant, cold-active lipase (lipa) from psychrotrophic yersinia enterocolitica. | a novel cold-active lipase gene encoding 294 amino acid residues was obtained from the yersinia enterocolitica strain km1. sequence alignment and phylogenetic analysis revealed that this novel lipase is a new member of the bacterial lipase family i.1. the lipase shares the conserved gxsxg motif and catalytic triad ser85-asp239-his261. the recombinant protein lipa was solubly and heterogeneously expressed in escherichia coli, purified by ni-affinity chromatography, and then characterized. lipa wa ... | 2015 | 26256062 |
| heat-killed lactobacillus spp. cells enhance survivals of caenorhabditis elegans against salmonella and yersinia infections. | this study examined the effect of feeding heat-killed lactobacillus cells on the survival of caenorhabditis elegans nematodes after salmonella typhimurium and yersinia enterocolitica infection. the feeding of heat-killed lactobacillus plantarum 133 (lp133) and lactobacillus fermentum 21 (lp21) cells to nematodes was shown to significantly increase the survival rate as well as stimulate the expression of pmk-1 gene that key factor for c. elegans immunity upon infection compared with control nemat ... | 2015 | 26250615 |
| genome-wide screening identifies six genes that are associated with susceptibility to escherichia coli microcin pdi. | the microcin pdi inhibits a diverse group of pathogenic escherichia coli strains. coculture of a single-gene knockout library (bw25113; n=3,985 mutants) against a microcin pdi-producing strain (e. coli 25) identified six mutants that were not susceptible (δatpa, δatpf, δdsba, δdsbb, δompf, and δompr). complementation of these genes restored susceptibility in all cases, and the loss of susceptibility was confirmed through independent gene knockouts in e. coli o157:h7 sakai. heterologous expressio ... | 2015 | 26209678 |
| production of a new non-specific nuclease from yersinia enterocolitica subsp. palearctica: optimization of induction conditions using response surface methodology. | a new non-specific nuclease from yersinia enterocolitica subsp. palearctica (y. nsn) was expressed in escherichia coli (e. coli) bl 21 star(tm) (de3)plyss. induction conditions, including isopropyl-β-d-thiogalactoside (iptg) concentration, cell density (od600), induction time and induction temperature, were optimized using response surface methodology. statistical analysis of the results revealed that induction temperature and all the quadratic terms of variables had significant effects on enzym ... | 2014 | 26019543 |
| [water-borne outbreak of yersinia enterocolitica o8 due to a small scale water system]. | a water-borne outbreak of yersinia enterocolitica o:8 associated with a small-scale water system occurred during july-august 2011 in toyama prefecture, japan. escherichia coli was not detected in tap water from the small-scale water system. however, the maximum concentration of viable bacteria in the tap water was 700cfu/ml, which exceeds the legal standard for purity of tap water (100cfu/ml). furthermore, y. enterocolitica o8 was isolated from the tap water with the use of immunomagnetic beads ... | 2014 | 25764804 |
| the expression of propionicin plg-1 gene (plg-1) by lactic starters. | propionicin plg-1 is a bacteriocin produced by propionibacterium thoenii p127. such bacteriocin inhibits wide range of food-borne pathogens such as pathogenic escherichia coli, pseudomonas aeruginosa, vibrio parahaemolyticus, yersinia enterocolitica and a strain of corynebacterium sp. in the present study, plg-1 gene expressing propionicin plg-1 was isolated, sequenced for the first time and the resulting sequence was analysed using several web-based bioinformatics programs. the pcr product cont ... | 2015 | 25684078 |
| impact of aeromonas and diarrheagenic escherichia coli screening in patients with diarrhea in paraná, southern brazil. | a wide diversity of bacterial agents may cause diarrhea, presenting challenges to clinical laboratories to define a diagnosis. considering that most stool cultures are negative, we screened stool samples from patients with diarrhea for the presence of 14 bacterial enteropathogens, aiming to establish which of them should be included in routine stool analysis. | 2014 | 25500659 |
| absence of ybey rnase compromises the growth and enhances the virulence plasmid gene expression of yersinia enterocolitica o:3. | ybey was recently recognized as an endoribonuclease playing a role in ribosome biosynthesis. in escherichia coli it functions as a single-strand-specific rnase that processes the 3' end of the 16s rrna and is crucial for the late-stage 70s ribosome quality control system. here we report that ybey is not essential in yersinia enterocolitica serotype o:3, yet its absence strongly compromised the bacterium. the lack of ybey resulted in misprocessing of 16s rrna and a severe decrease of growth rate ... | 2015 | 25416689 |
| a new series of antibacterial nitrosopyrimidines: synthesis and structure-activity relationship. | new nitrosopyrimidines were synthesized and evaluated as potential antibacterial agents. different compounds structurally related with 4,6-bis(alkyl or arylamino)-5-nitrosopyrimidines were evaluated. some of these nitrosopyrimidines displayed significant antibacterial activity against human pathogenic bacteria. among them compounds 1c, 2a-c, and 9a-c exhibited remarkable activity against methicillin-sensitive and -resistant staphylococcus aureus, escherichia coli, yersinia enterocolitica, and sa ... | 2015 | 25413501 |
| expanding the results of a high throughput screen against an isochorismate-pyruvate lyase to enzymes of a similar scaffold or mechanism. | antibiotic resistance is a growing health concern, and new avenues of antimicrobial drug design are being actively sought. one suggested pathway to be targeted for inhibitor design is that of iron scavenging through siderophores. here we present a high throughput screen to the isochorismate-pyruvate lyase of pseudomonas aeruginosa, an enzyme required for the production of the siderophore pyochelin. compounds identified in the screen are high nanomolar to low micromolar inhibitors of the enzyme a ... | 2014 | 25282647 |
| best practices for planning events encouraging human-animal interactions. | educational events encouraging human-animal interaction include the risk of zoonotic disease transmission. it is estimated that 14% of all disease in the usa caused by campylobacter spp., cryptosporidium spp., shiga toxin-producing escherichia coli (stec) o157, non-o157 stecs, listeria monocytogenes, non-typhoidal salmonella enterica and yersinia enterocolitica were attributable to animal contact. this article reviews best practices for organizing events where human-animal interactions are encou ... | 2015 | 24751220 |
| rabbit monoclonal antibodies directed at the t3ss effector protein yopm identify human pathogenic yersinia isolates. | the yersinia outer protein m (yopm) is a type 3 secretion system (t3ss)-dependent effector protein of yersinia enterocolitica, yersinia pseudotuberculosis and yersinia pestis. although yopm is indispensable for full virulence, its molecular functions still remain largely elusive. recently, we could identify the recombinant yopm (ryopm) protein derived from the y. enterocolitica strain 8081 (jb580) as a cell-penetrating protein, which down-regulates the expression of various pro-inflammatory cyto ... | 2014 | 24636859 |
| in vitro antibacterial activity of several plant extracts and oils against some gram-negative bacteria. | medicinal plants are considered new resources for producing agents that could act as alternatives to antibiotics in the treatment of antibiotic-resistant bacteria. the aim of this study was to evaluate the antibacterial activity of 28 plant extracts and oils against four gram-negative bacterial species. | 2014 | 24453392 |
| evaluation and functional characterization of a biosurfactant produced by lactobacillus plantarum cfr 2194. | the study details the investigations on the ability of lactobacillus plantarum cfr 2194, an isolate from kanjika, a rice-based ayurvedic fermented product, to produce biosurfactant. surfactant production, as a function of fermentation time, indicates that the maximum production occurred at 72 h under stationary conditions. isolation, partial purification, and characterization of the biosurfactant produced have been carried out, and fourier transform infrared spectroscopy (ftir) spectra demonstra ... | 2014 | 24258794 |
| lysine221 is the general base residue of the isochorismate synthase from pseudomonas aeruginosa (pcha) in a reaction that is diffusion limited. | the isochorismate synthase from pseudomonas aeruginosa (pcha) catalyzes the conversion of chorismate to isochorismate, which is subsequently converted by a second enzyme (pchb) to salicylate for incorporation into the salicylate-capped siderophore pyochelin. pcha is a member of the mst family of enzymes, which includes the structurally homologous isochorismate synthases from escherichia coli (entc and menf) and salicylate synthases from yersinia enterocolitica (irp9) and mycobacterium tuberculos ... | 2013 | 23942051 |
| effects of subtherapeutic concentrations of antimicrobials on gene acquisition events in yersinia, proteus, shigella, and salmonella recipient organisms in isolated ligated intestinal loops of swine. | to assess antimicrobial resistance and transfer of virulence genes facilitated by subtherapeutic concentrations of antimicrobials in swine intestines. | 2013 | 23879845 |
| ompc-like porin from outer membrane of yersinia enterocolitica: molecular structure and functional activity. | ompc-like porin was isolated from the outer membrane (om) of yersinia enterocolitica cultured at 37°c (the "warm" variant) and its physicochemical and functional properties were studied. the amino acid sequence of ompc porin was established, and the primary structure and transmembrane topology of this protein were analyzed in comparison with the ompf porin isolated from y. enterocolitica cultured at 6°c (the "cold" variant). both porins of y. enterocolitica had a high homology degree (65%) betwe ... | 2013 | 23848152 |
| microbiological quality of fresh-cut carrots and process waters. | fresh vegetables may be contaminated by pathogens in different ways after harvest. pathogenic microorganisms associated with fresh vegetables can cause severe outbreaks of foodborne disease. we discuss here the results of microbiological analysis of carrot samples, as well as of washing, processing, and wastewater samples. washed, unpeeled carrots generally contained the highest aerobic plate counts (mean, 5.5 log cfu/g). escherichia coli was not detected in any carrot or water sample examined. ... | 2013 | 23834799 |
| synergistic effect between colistin and bacteriocins in controlling gram-negative pathogens and their potential to reduce antibiotic toxicity in mammalian epithelial cells. | pathogens resistant to most conventional antibiotics are a harbinger of the need to discover novel antimicrobials and anti-infective agents and develop innovative strategies to combat them. the aim of this study was to assess the in vitro activity of colistin alone or in combination with two bacteriocins, nisin a and pediocin pa-1/ach, against salmonella choleraesuis atcc 14028, pseudomonas aeruginosa atcc 27853, yersinia enterocolitica atcc 9610, and escherichia coli atcc 35150 (o157:h7). the s ... | 2013 | 23571533 |
| bacterial growth in red blood cell units exposed to uncontrolled temperatures: challenging the 30-minute rule. | the '30-min rule' requires discarding red blood cells (rbcs) exposed to uncontrolled temperatures for >30 min to ensure safe rbc transfusion. this study was aimed at determining whether multiple room temperature (rt) exposures promote bacterial growth. | 2013 | 23398307 |
| recombinant production of yersinia enterocolitica pyruvate kinase isoenzymes pyka and pykf. | the glycolytic enzyme pyruvate kinase (pk) generates atp from adp through substrate-level phosphorylation powered by the conversion of phosphoenolpyruvate to pyruvate. in contrast to other bacteria, enterobacteriaceae, such as pathogenic yersiniae, harbour two pyruvate kinases encoded by pyka and pykf. the individual roles of these isoenzymes are poorly understood. in an attempt to make the yersinia enterocolitica pyruvate kinases pyka and pykf amenable to structural and functional characterisat ... | 2013 | 23384479 |
| estimates of illnesses, hospitalizations and deaths caused by major bacterial enteric pathogens in young children in the united states. | many enteric pathogens disproportionately affect young children. however, higher incidences of laboratory-confirmed illness may be explained, at least in part, by higher rates of medical care-seeking and stool sample submission in this age group. we estimated the overall number of bacterial enteric illnesses among children <5 years old in the united states caused by campylobacter, escherichia coli o157, nontyphoidal salmonella, shigella and yersinia enterocolitica. | 2013 | 23249909 |
| essential oil of azorella cryptantha collected in two different locations from san juan province, argentina: chemical variability and anti-insect and antimicrobial activities. | the essential oils (eos) of two populations of azorella cryptantha (clos) reiche, a native species from san juan province, were obtained by hydrodistillation in a clevenger-type apparatus and characterized by gc-fid and gc/ms analyses. the compounds identified amounted to 92.3 and 88.7% of the total oil composition for a. cryptantha from bauchaceta (ac-bau) and agua negra (ac-an), respectively. the eo composition for the two populations was similar, although with differences in the identity and ... | 2012 | 22899606 |
| estimates of enteric illness attributable to contact with animals and their environments in the united states. | contact with animals and their environment is an important, and often preventable, route of transmission for enteric pathogens. this study estimated the annual burden of illness attributable to animal contact for 7 groups of pathogens: campylobacter species, cryptosporidium species, shiga toxin-producing escherichia coli (stec) o157, stec non-o157, listeria monocytogenes, nontyphoidal salmonella species, and yersinia enterocolitica. | 2012 | 22572672 |
| nahua indian medicinal plants (mexico): inhibitory activity on nf-κb as an anti-inflammatory model and antibacterial effects. | selected plants documented as medicinal in an ethnobotanical study with the nahua of the sierra de zongolica (veracruz, mexico) were evaluated for anti-inflammatory and antibacterial activity. one of the potential sides of action of anti-inflammatory drugs is the transcription factor nf-κb. this factor is essential for the immune, inflammatory, and acute phase responses. we therefore tested extracts from a total of 28 plants used by the nahua indians for their potential effect on the activation ... | 1996 | 23195081 |
| establishment of serological herd profiles for zoonoses and production diseases in pigs by "meat juice multi-serology". | the most important pork-borne zoonotic diseases in humans such as salmonelloses and yersinioses cause only latent infections in pigs. thus, the infection of pigs does not result in apparent or palpable alterations in the pig carcasses. this is the major reason, why the traditional meat inspection with adspection, palpation and incision is not able to control the food safety risks of today. the objective of this paper is to evaluate a set of serological tests, which provides a classification of p ... | 2014 | 24411983 |
| urticaria and bacterial infections. | the association between urticaria and infectious diseases has been discussed for >100 years. however, a causal relationship with underlying or precipitating infection is difficult to establish. the purpose of this work was to perform a systematic analysis of the published cases of urticaria associated with bacterial infections. we give an umbrella breakdown of up-to-date systematic reviews and other important publications on the complex association of urticaria and bacterial infections. we did a ... | 2016 | 24857191 |
| differential modulation of intracellular survival of cytosolic and vacuolar pathogens by curcumin. | curcumin, a principal component of turmeric, acts as an immunomodulator regulating the host defenses in response to a diseased condition. the role of curcumin in controlling certain infectious diseases is highly controversial. it is known to alleviate symptoms of helicobacter pylori infection and exacerbate that of leishmania infection. we have evaluated the role of curcumin in modulating the fate of various intracellular bacterial pathogens. we show that pretreatment of macrophages with curcumi ... | 2012 | 22890770 |
| occurrence and analysis of irp2 virulence gene in isolates of klebsiella pneumoniae and enterobacter spp. from microbiota and hospital and community-acquired infections. | eighty-five isolates of klebsiella pneumoniae and enterobacter spp., originating from hospital- and community-acquired infections and from oropharyngeal and faecal microbiota from patients in recife-pe, brazil, were analyzed regarding the presence of irp2 gene. this is a yersinia typical gene involved in the synthesis of siderophore yersiniabactin. dna sequencing confirmed the identity of irp2 gene in five k. pneumoniae, five enterobacter aerogenes and one enterobacter amnigenus isolates. to our ... | 2016 | 27133266 |
| chemical composition and biological activity of the centipeda minima (asteraceae). | the antimicrobial activity of the centipeda minima l. (asteraceae) extract was evaluated against seven microorganisms using the disc diffusion method. the extract showed a broad spectrum of antimicrobial activity against all the tested bacterial strains, especially enterobacter aerogenes, klebsiella pneumonia, staphylococcus aureus and yersinia enterocolitica. the chemical composition of the extract obtained from centipeda minima was analysed by gc-ms. twentythree compounds, constituting about 8 ... | 2007 | 22692191 |
| evaluation of drug-resistant enterobacteriaceae in retail poultry and beef. | there has been increasing concern on the emergence of multidrug-resistant foodborne pathogens from foods of animal origin, including poultry. the current study aimed to evaluate antibiotic-resistant enterobacteriaceae from raw retail chicken/turkey parts (thigh, wings, breast, and ground) and beef meat (ground and chunks) in middle tennessee. resistance of the collected enterobacteriaceae to a panel of antibiotics was determined by the kirby-bauer disk diffusion test. retail meats were also assa ... | 2013 | 23472034 |
| lack of correlation between in vitro antibiosis and in vivo protection against enteropathogenic bacteria by probiotic lactobacilli. | increased resistance to infection is one of the beneficial effects attributed to probiotic microorganisms. this effect may be due to several mechanisms: production of inhibitory substances, blocking of adhesion sites on the intestinal surface, competition for nutrients and stimulation of mucosal and systemic immunity. the present study aimed to investigate the correlation between in vitro and in vivo antimicrobial activity of probiotic lactobacilli. the agar spot test was used to show that twent ... | 2014 | 24140789 |
| in vitro probiotic characterization of lactobacillus strains from fermented radish and their anti-adherence activity against enteric pathogens. | in this study, we evaluated the probiotic properties of lactobacillus plantarum, lactobacillus pentosus, and lactobacillus fermentum strains isolated from fermented radish. all the strains survived the simulated oro-gastrointestinal transit condition and showed significantly higher adherence to caco-2 cells compared with the probiotic strain lactobacillus rhamnosus gg. the strains showed broad-spectrum antimicrobial activity, autoaggregation, and coaggregation capacity with pathogens. furthermor ... | 2015 | 26382558 |
| antibacterial activity of lactobacillus spp. isolated from the feces of healthy infants against enteropathogenic bacteria. | lactobacilli are normal microflora of the gastrointestinal (gi) tract and are a heterogeneous group of lactic acid bacteria (lab). lactobacillus strains with probiotic activity may have health benefits for human. this study investigates the probiotic potential of lactobacillus strains obtained from the feces of healthy infants and also explores antibacterial activity of lactobacillus strains with probiotic potential against enteropathogenic bacteria. fecal samples were collected from 95 healthy ... | 2015 | 25930687 |
| technological and probiotic potential of bgra43 a natural isolate of lactobacillus helveticus. | lactobacillus helveticus bgra43 is a human intestinal isolate showing antimicrobial activity, amongst others, against yersinia enterocolitica, shigella sonnei, shigella flexneri, and streptococcus pneumoniae. bgra43 produces prth proteinase with proteolytic activity on both casein and β-lactoglobulin (blg). bgra43 is able to reduce the allergenicity of blg. bioactive peptides released in bgra43 fermented milk are potent modulators of innate immunity by modulating the production of proinflammator ... | 2013 | 23346083 |
| biological potency and characterization of antibacterial substances produced by lactobacillus pentosus isolated from hentak, a fermented fish product of north-east india. | lactic acid bacteria (lab) isolated from various foods are important due to their potential to inhibit microorganisms, including drug-resistant bacteria. the objectives of this investigation were to isolate and identify antibacterial substances producing lab from hentak, a traditional fermented fish product of manipur (north-east india), and to optimize the production of antagonistic substances present in cell free neutralized supernatant (cfns) against enteric bacterial pathogens using the 'one ... | 2016 | 27795886 |
| a lactobacillus plantarum strain isolated from kefir protects against intestinal infection with yersinia enterocolitica o9 and modulates immunity in mice. | lactobacillus plantarum c4, previously isolated from kefir and characterized as a potential probiotic strain, was tested for its protective and immunomodulatory capacity in a murine model of yersiniosis. the inoculation of balb/c mice with a low pathogenicity serotype o9 strain of yersinia enterocolitica results in a prolonged intestinal infection with colonization of peyer's patches. pretreatment with c4 was without effect on fecal excretion of yersiniae, but shortened the colonization of peyer ... | 2015 | 26272025 |
| predicting inhibition and inactivation of yersinia enterocolitica through lactic acid production by lactobacillus sakei. | in food technology, there is a need for models taking into account the interactions between microorganisms, in order to correctly predict the safety and shelf life of food products. when leaving these interactions out of consideration, a discrepancy between the model prediction and the actual microbial evolution may occur for certain types of food products. in this study, a model describing the inhibition of the pathogenic yersinia enterocolitica in mono- and coculture with lactobacillus sakei w ... | 2003 | 24757785 |
| biophysical characterization of the type iii secretion tip proteins and the tip proteins attached to bacterium-like particles. | bacterium-like particles (blps), derived from lactococcus lactis, offer a self-adjuvanting delivery vehicle for subunit protein vaccines. proteins can be specifically loaded onto the blps via a peptidoglycan anchoring (pa) domain. in this study, the tip proteins ipad, sipd, and lcrv belonging to type iii secretion systems of shigella flexneri, salmonella enterica, and yersinia enterocolitica, respectively, were fused to the pa and loaded onto the blps. herein, we biophysically characterized thes ... | 2015 | 24916512 |
| prevalence and antimicrobial resistance of listeria, salmonella, and yersinia species isolates in ducks and geese. | the aims of this study were to determine the prevalence and antimicrobial resistance of listeria, salmonella, and yersinia spp. isolated from duck and goose intestinal contents. a total of 471 samples, including 291 duck and 180 goose intestinal contents, were purchased from wet markets between november 2008 and july 2010. listeria, salmonella, and yersinia spp. were isolated from 58 (12.3%), 107 (22.7%), and 80 (17%) of the samples, respectively. it was concluded that listeria ivanovii, salmone ... | 2014 | 24706981 |
| fate of listeria monocytogenes , pathogenic yersinia enterocolitica , and escherichia coli o157:h7 gfp(+) in ready-to-eat salad during cold storage: what is the risk to consumers? | in this study, we investigated the fate of listeria monocytogenes , pathogenic yersinia enterocolitica , and escherichia coli o157:h7 gfp(+) inoculated in low numbers into ready-to-eat baby spinach and mixed-ingredient salad (baby spinach with chicken meat). samples were stored at recommended maximum refrigerator temperature (8°c in sweden) or at an abuse temperature (15°c) for up to 7 days. mixed-ingredient salad supported considerable growth when stored at 15°c during shelf life (3 days), with ... | 2017 | 28221975 |
| propagation method for persistent high yield of diverse listeria phages on permissive hosts at refrigeration temperatures. | the efficient production of a high concentration of bacteriophage in large volumes has been a limiting factor in the exploration of the true potential of these organisms for biotechnology, agriculture and medicine. traditional methods focus on generating small volumes of highly concentrated samples as the end product of extensive mechanical and osmotic processing. to function at an industrial scale mandates extensive investment in infrastructure and input materials not feasible for many smaller ... | 2016 | 27287043 |
| predicting the kinetics of listeria monocytogenes and yersinia enterocolitica under dynamic growth/death-inducing conditions, in italian style fresh sausage. | traditional italian pork products can be consumed after variable drying periods, where the temporal decrease of water activity spans from optimal to inactivating values. this makes it necessary to a) consider the bias factor when applying culture-medium-based predictive models to sausage; b) apply the dynamic version (described by differential equations) of those models; c) combine growth and death models in a continuous way, including the highly uncertain growth/no growth range separating the t ... | 2017 | 27178365 |
| free-living species of carnivorous mammals in poland: red fox, beech marten, and raccoon as a potential reservoir of salmonella, yersinia, listeria spp. and coagulase-positive staphylococcus. | the objective of the study was to examine a population of free-living carnivorous mammals most commonly found in poland (red fox, beech marten, and raccoon) for the occurrence of bacteria that are potentially pathogenic for humans and other animal species and to determine their virulence potential (the presence of selected virulence genes). from the total pool of isolates obtained (n = 328), we selected 90 belonging to species that pose the greatest potential threat to human health: salmonella s ... | 2016 | 27171434 |
| prevalence of foodborne pathogens in freshwater fish in latvia. | the aim of this study was to detect the prevalence of salmonella spp., listeria monocytogenes, and yersinia enterocolitica in freshwater fish in latvia. in total, 235 samples, including freshly caught fish from fives lakes (n = 129) and fish from retail markets (n = 106), were collected from april 2014 to december 2014 in latvia. samples were tested according to international organization for standardization methods. no salmonella spp. were found in fresh fish from lakes or in commercially avail ... | 2015 | 26555535 |
| microbiological survey of raw and ready-to-eat leafy green vegetables marketed in italy. | the presence of foodborne pathogens (salmonella spp., listeria monocytogenes, escherichia coli o157:h7, thermotolerant campylobacter, yersinia enterocolitica and norovirus) in fresh leafy (fl) and ready-to-eat (rte) vegetable products, sampled at random on the italian market, was investigated to evaluate the level of risk to consumers. nine regional laboratories, representing 18 of the 20 regions of italy and in which 97.7% of the country's population resides, were involved in this study. all la ... | 2015 | 26114592 |
| the antimicrobial activity of liquidambar orientalis mill. against food pathogens and antioxidant capacity of leaf extracts. | medicinal plants are an important source of substances which are claimed to induce antimicrobial, antimutagenic and antioxidant effects. many plants have been used due to their antimicrobial treatments. antimicrobial and antioxidant activities of l. orientalis have not been reported to the present day. the aim of this work was to investigate of the antimicrobial and antioxidant potentials of different extracts from l. orientalis. | 2014 | 25395700 |
| definition of a standard protocol to determine the growth potential of listeria monotgenes and yersinia enterocolitica in pork sausage produced in abruzzo region, italy. | pork meat products consumed raw or after a short period of fermentation can be considered at risk for food safety. sausages (fresh sausage made from pork meat) are produced in several italian regions, with variation in ingredients. in some italian regions, including abruzzo, these products are frequently consumed raw or undercooked, after a variable period of fermentation. the european community food regulation promotes the use of challenge tests to determine safety levels. this study is aimed t ... | 2015 | 27800415 |
| crystal structure of an engineered yopm-inlb hybrid protein. | the multi-domain protein inlb (internalin b) from listeria monocytogenes is an agonist of the human receptor tyrosine kinase met. only the internalin domain directly interacts with met. the internalin domain consists of seven central leucine-rich repeats (lrrs) flanked by an n-terminal helical cap domain and a c-terminal immunoglobulin-like structure. a potential function of the n-terminal cap in receptor binding could so far not be demonstrated by deleting the cap, since the cap is also implica ... | 2014 | 24669959 |
| yersinia enterocolitica inhibits salmonella enterica serovar typhimurium and listeria monocytogenes cellular uptake. | yersinia enterocolitica biovar 1b employs two type three secretion systems (t3ss), ysa and ysc, which inject effector proteins into macrophages to prevent phagocytosis. conversely, salmonella enterica serovar typhimurium uses a t3ss encoded by salmonella pathogenicity island 1 (spi1) to actively invade cells that are normally nonphagocytic and a second t3ss encoded by spi2 to survive within macrophages. given the distinctly different outcomes that occur with regard to host cell uptake of s. typh ... | 2014 | 24126528 |
| cytotoxic and bioactive properties of different color tulip flowers and degradation kinetic of tulip flower anthocyanins. | this study was conducted to determine the potential use of anthocyanin-based extracts (abes) of wasted tulip flowers as food/drug colorants. for this aim, wasted tulip flowers were samples and analyzed for their bioactive properties and cytotoxicity. total phenolic contents of the extracts of the claret red (126.55 mg of gallic acid equivalent (gae)/g dry extract) and orange-red (113.76 mg gae/g dry extract) flowers were the higher than those of the other tulip flowers. total anthocyanin levels ... | 2013 | 23712096 |
| development of a random genomic dna microarray for the detection and identification of listeria monocytogenes in milk. | we developed a dna microarray that contains random genomic dna fragments of listeria monocytogenes, validated its diagnostic abilities using cells grown in laboratory media and milk, and established enrichment conditions for detection of a low population of l. monocytogenes in milk. genomic dna of l. monocytogenes strain atcc 19111 was fractionated by agarose gel electrophoresis after being cleaved using several different pairs of restriction enzymes. sixty dna fragments of different sizes were ... | 2013 | 23313851 |
| identification of the crp gene in avian pasteurella multocida and evaluation of the effects of crp deletion on its phenotype, virulence and immunogenicity. | pasteurella multocida (p. multocida) is an important veterinary pathogen that can cause severe diseases in a wide range of mammals and birds. the global regulator crp gene has been found to regulate the virulence of some bacteria, and crp mutants have been demonstrated to be effective attenuated vaccines against salmonella enterica and yersinia enterocolitica. here, we first characterized the crp gene in p. multocida, and we report the effects of a crp deletion. | 2016 | 27343075 |
| a prevalence study of salmonella spp., yersinia spp., toxoplasma gondii and porcine reproductive and respiratory syndrome virus in uk pigs at slaughter. | an abattoir-based study was undertaken between january and may 2013 to estimate the prevalence of salmonella spp. and yersinia spp. carriage and seroprevalence of antibodies to toxoplasma gondii and porcine reproductive and respiratory syndrome virus (prrsv) in uk pigs at slaughter. in total, 626 pigs were sampled at 14 abattoirs that together process 80% of the annual uk pig slaughter throughput. sampling was weighted by abattoir throughput and sampling dates and pig carcasses were randomly sel ... | 2016 | 26586451 |
| yersinia enterocolitica in sheep--a high frequency of biotype 1a. | pigs are regarded as the main reservoir for human pathogenic yersinia enterocolitica, which is dominated by bioserotype 4/o:3. other animals, including sheep, have occasionally been reported as carriers of pathogenic strains of y. enterocolitica. to our knowledge, this is the first study performed in the nordic countries in which the presence of y. enterocolitica in sheep is investigated. | 2012 | 22748116 |
| holo structure and steady state kinetics of the thiazolinyl imine reductases for siderophore biosynthesis. | thiazolinyl imine reductases catalyze the nadph-dependent reduction of a thiazoline to a thiazolidine, a required step in the formation of the siderophores yersiniabactin (yersinia spp.) and pyochelin (pseudomonas aeruginosa). these stand-alone nonribosomal peptide tailoring domains are structural homologues of sugar oxidoreductases. two closed structures of the thiazolinyl imine reductase from yersinia enterocolitica (irp3) are presented here: an nadp(+)-bound structure to 1.45 å resolution and ... | 2016 | 27601130 |
| expedient route to access rare deoxy amino l-sugar building blocks for the assembly of bacterial glycoconjugates. | bacterial glycoproteins and oligosaccharides contain several rare deoxy amino l-sugars which are virtually absent in the human cells. this structural difference between the bacterial and host cell surface glycans can be exploited for the development of carbohydrate based vaccines and target specific drugs. however, the unusual deoxy amino l-sugars present in the bacterial glycoconjugates are not available from natural sources. thus, procurement of orthogonally protected rare l-sugar building blo ... | 2016 | 27002789 |
| surface-modified yeast cells: a novel eukaryotic carrier for oral application. | the effective targeting and subsequent binding of particulate carriers to m cells in peyer's patches of the gut is a prerequisite for the development of oral delivery systems. we have established a novel carrier system based on cell surface expression of the β1-integrin binding domain of invasins derived from yersinia enterocolitica and yersinia pseudotuberculosis on the yeast saccharomyces cerevisiae. all invasin derivatives were shown to be effectively expressed on the cell surface and recombi ... | 2016 | 26763373 |
| simultaneous occurrence of salmonella enterica, campylobacter spp. and yersinia enterocolitica along the pork production chain from farm to meat processing in five conventional fattening pig herds in lower saxony. | the objectives of this study were to gather data on the occurrence of salmonella (s.) enterica, campylobacter spp. and yersinia (y.) enterocolitica along the pork production chain and to further analyze detected salmonella isolates by additionally applying mlva (multiple-locus variable-number tandem repeat analysis). in total, 336 samples were collected at primary production, slaughter and meat processing from five conventional fattening pig farms and one common slaughterhouse. at farm level, s. ... | 2016 | 27529991 |
| genome sequence of the fish pathogen yersinia ruckeri sc09 provides insights into niche adaptation and pathogenic mechanism. | yersinia ruckeri is the etiologic agent of enteric red mouth disease (erm), a severe fish disease prevailing in worldwide aquaculture industries. here we report for the first time the complete genome of y. ruckeri (yersinia ruckeri) sc09, a highly virulent strain isolated from ictalurus punctatus with severe septicemia. sc09 possesses a single chromosome of 3,923,491 base pairs, which contains 3651 predicted protein coding sequences (cds), 19 rrna genes, and 79 trna genes. among the cds, we have ... | 2016 | 27089334 |
| activity of a bacterial cell envelope stress response is controlled by the interaction of a protein binding domain with different partners. | the bacterial phage shock protein (psp) system is a highly conserved cell envelope stress response required for virulence in yersinia enterocolitica and salmonella enterica. in non-inducing conditions the transcription factor pspf is inhibited by an interaction with pspa. in contrast, pspa associates with the cytoplasmic membrane proteins pspbc during inducing conditions. this has led to the proposal that pspbc exists in an off state, which cannot recruit pspa, or an on state, which can. however ... | 2015 | 25802329 |
| presence of salmonella spp., yersinia enterocolitica, yersinia pseudotuberculosis and escherichia coli o157:h7 in wild boars. | the european wild boar populations are growing and spreading to new areas, which might constitute a threat to public health, since wild boar can harbour pathogens with the potential to cause serious illness in humans. tonsils, ileocaecal lymph nodes and faecal samples were collected from 88 swedish wild boars and analysed for the presence of the zoonotic pathogens salmonella spp., yersinia enterocolitica, y. pseudotuberculosis and enterohaemorrhagic escherichia coli o157:h7 (ehec). a combination ... | 2014 | 24512817 |
| campylobacter spp., yersinia enterocolitica, and salmonella enterica and their simultaneous occurrence in german fattening pig herds and their environment. | campylobacter spp., salmonella enterica, and yersinia enterocolitica are common causes of foodborne infections in humans with pork as a potential source. monitoring programs at farm level are, to date, only implemented for s. enterica, while epidemiological knowledge of the other two pathogens is still lacking. this study aimed to assess the pathogen load (in the pigs' environment) in fattening pig herds, their simultaneous occurrence, and the occurrence of campylobacter spp. and y. enterocoliti ... | 2013 | 24112569 |
| prevalence, characterization and antimicrobial susceptibility of salmonella enterica and yersinia enterocolitica in pigs at slaughter in italy. | in 2005-2008, 1152 samples (451 faecal samples, 451 carcass swabs and 250 tonsils) were collected from 451 finishing pigs slaughtered in three abattoirs of northern italy. in two abattoirs, 34 scalding water samples were collected. the aim of this study was to investigate the faecal and palatine tonsil carriage rate of salmonella enterica and yersinia enterocolitica in pigs at slaughter and the degree of carcass contamination by these bacteria. typing of the isolates, virulence characterization ... | 2013 | 23603278 |
| novel flow cytometric screening method for bacterial contamination of red blood cells: a proof-of-principle evaluation. | platelet concentrates (pcs) are the major source of transfusion-transmitted bacterial infection but the significantly higher number of transfused red blood cells (rbcs) has resulted in a similar occurrence of possible transfusion-associated bacterial infections as those of recipients transfused with platelets. the aim of this study was adaption of the bactiflow (bf) flow cytometric rapid pc screening method for the detection of bacterial contamination in rbcs. | 2014 | 24304098 |
| in situ structural analysis of the yersinia enterocolitica injectisome. | injectisomes are multi-protein transmembrane machines allowing pathogenic bacteria to inject effector proteins into eukaryotic host cells, a process called type iii secretion. here we present the first three-dimensional structure of yersinia enterocolitica and shigella flexneri injectisomes in situ and the first structural analysis of the yersinia injectisome. unexpectedly, basal bodies of injectisomes inside the bacterial cells showed length variations of 20%. the in situ structures of the y. e ... | 2013 | 23908767 |
| primary cellulitis and cutaneous abscess caused by yersinia enterocolitica in an immunocompetent host: a case report and literature review. | primary extraintestinal complications caused by yersinia enterocolitica are extremely rare, especially in the form of skin and soft-tissue manifestations, and little is known about their clinical characteristics and treatments. we presented our case and reviewed past cases of primary skin and soft-tissue infections caused by y enterocolitica. we report a case of primary cellulitis and cutaneous abscess caused by y enterocolitica in an immunocompetent 70-year-old woman with keratodermia tylodes p ... | 2016 | 27368001 |
| yersinia enterocolitica and photorhabdus asymbiotica β-lactamases blaa are exported by the twin-arginine translocation pathway. | in general, β-lactamases of medically important gram-negative bacteria are sec-dependently translocated into the periplasm. in contrast, β-lactamases of mycobacteria spp. (blac, blas) and the gram-negative environmental bacteria stenotrophomonas maltophilia (l2) and xanthomonas campestris (bla(xcc-1)) have been reported to be secreted by the twin-arginine translocation (tat) system. yersinia enterocolitica carries 2 distinct β-lactamase genes (blaa and blab) encoding blaa(ye) and the ampc-like β ... | 2013 | 23276548 |
| meat juice serology and improved food chain information as control tools for pork-related public health hazards. | the seroprevalence of salmonella spp., pathogenic yersinia spp., toxoplasma gondii and trichinella spp. was studied in 1353 finishing pigs from 259 farms that were allocated according to farm types: large fattening farms (≥ 1000 pig places), small fattening farms (< 1000 pig places) and farrow-to-finish farms. the antibodies were analysed with commercial elisa kits in meat juice samples that were collected at finnish slaughterhouses. salmonella antibodies were rare (3% of pigs, 14% of farms) whe ... | 2015 | 25439544 |
| antibiotic resistant shigella is a major cause of diarrhoea in the highlands of papua new guinea. | diarrhoea remains a major cause of illness in papua new guinea (png); however, little is known about its aetiology. as a result of the cholera outbreak that spread throughout png in 2009-2011, we conducted diarrhoeal surveillance in eastern highlands province. | 2014 | 25390051 |
| a study of single nucleotide polymorphism in the ystb gene of yersinia enterocolitica strains isolated from various wild animal species. | y. enterocolitica is the causative agent of yersiniosis. the objective of the article was a study of single nucleotide polymorphism in the ystb gene of y. enterocolitica strains isolated from various wild animal species. | 2017 | 28378975 |
| yersinia spp. in wild rodents and shrews in finland. | yersinia enterocolitica and yersinia pseudotuberculosis are important zoonotic bacteria causing human enteric yersiniosis commonly reported in europe. all y. pseudotuberculosis strains are considered pathogenic, while y. enterocolitica include both pathogenic and nonpathogenic strains which can be divided into six biotypes (1a, 1b, and 2-5) and about 30 serotypes. the most common types causing yersiniosis in europe are y. enterocolitica bioserotypes 4/o:3 and 2/o:9. strains belonging to biotype ... | 2017 | 28332937 |
| presence of ail and ystb genes in yersinia enterocolitica biotype 1a isolates from game animals in poland. | the pathogenicity of yersinia enterocolitica is associated with the presence of plasmid and chromosomal virulence genes. strains belonging to biotype 1a do not possess pyv plasmids, often harbour the ystb gene and usually lack the ail gene, which is the main virulence marker for y. enterocolitica. the simultaneous presence of ail and ystb is uncommon. in this study, 21/218 (9.6%) biotype 1a y. enterocolitica isolates from rectal swabs of wild boar (sus scrofa; n = 18), red deer (cervus elaphus; ... | 2017 | 28283072 |
| interfering with outer membrane biogenesis to fight gram-negative bacterial pathogens. | | 2017 | 28277902 |
| henoch-schönlein purpura following yersinia enterocolitica infection. | | 2017 | 28253462 |
| luxcde-luxab-based promoter reporter system to monitor the yersinia enterocolitica o:3 gene expression in vivo. | it is crucial to understand the in vitro and in vivo regulation of the virulence factor genes of bacterial pathogens. in this study, we describe the construction of a versatile reporter system for yersinia enterocolitica serotype o:3 (yeo3) based on the luxcdabe operon. in strain yeo3-luxcde we integrated the luciferase substrate biosynthetic genes, luxcde, into the genome of the bacterium so that the substrate is constitutively produced. the luxab genes that encode the luciferase enzyme were cl ... | 2017 | 28235077 |
| synthesis of β-(1→2)-linked 6-deoxy-l-altropyranose oligosaccharides via gold(i)-catalyzed glycosylation of an ortho-hexynylbenzoate donor. | the β-(1→2)-linked 6-deoxy-l-altropyranose di- to pentasaccharides 2-5, relevant to the o-antigen of the infectious yersinia enterocolitica o:3, were synthesized for the first time. the challenging 1,2-cis-altropyranosyl linkage was assembled effectively via glycosylation with 2-o-benzyl-3,4-di-o-benzoyl-6-deoxy-l-altropyranosyl ortho-hexynylbenzoate (7) under the catalysis of pph3auntf2. nmr and molecular modeling studies showed that the pentasaccharide (5) adopted a left-handed helical conform ... | 2017 | 28230982 |
| yersinia enterocolitica ileitis mimicking pediatric crohn's disease. | | 2017 | 28230560 |
| allograft inflammatory factor 1 is a regulator of transcytosis in m cells. | m cells in follicle-associated epithelium (fae) are specialized antigen-sampling cells that take up intestinal luminal antigens. transcription factor spi-b regulates m-cell maturation, but the molecules that promote transcytosis within m cells are not fully identified. here we show that mouse allograft inflammatory factor 1 (aif1) is expressed by m cells and contributes to m-cell transcytosis. fae in aif1(-/-) mice has suppressed uptake of particles and commensal bacteria, compared with wild-typ ... | 2017 | 28224999 |
| control of human pathogenic yersinia enterocolitica in minced meat: comparative analysis of different interventions using a risk assessment approach. | this study aimed to evaluate the effect of different processing scenarios along the farm-to-fork chain on the contamination of minced pork with human pathogenic y. enterocolitica. a modular process risk model (mprm) was used to perform the assessment of the concentrations of pathogenic y. enterocolitica in minced meat produced in industrial meat processing plants. the model described the production of minced pork starting from the contamination of pig carcasses with pathogenic y. enterocolitica ... | 2017 | 28213039 |
| rapid cd8(+) function is critical for protection of neonatal mice from an extracellular bacterial enteropathogen. | both human and murine neonates are characteristically highly susceptible to bacterial infections. however, we recently discovered that neonatal mice are surprisingly highly resistant to oral infection with yersinia enterocolitica. this resistance was linked with activation of both innate and adaptive responses, involving innate phagocytes, cd4(+) cells, and b cells. we have now extended these studies and found that cd8(+) cells also contribute importantly to neonatal protection from y. enterocol ... | 2016 | 28119902 |
| identifying components required for omp biogenesis as novel targets for antiinfective drugs. | the emergence of multiresistant gram-negative bacteria requires new therapies for combating bacterial infections. targeting the biogenesis of virulence factors could be an alternative strategy instead of killing bacteria with antibiotics. the outer membrane (om) of gram-negative bacteria acts as a physical barrier. at the same time it facilitates the exchange of molecules and harbors a multitude of proteins associated with virulence. in order to insert proteins into the om, an essential oligomer ... | 2017 | 28118090 |
| yersinia enterocolitica, a neglected cause of human enteric infections in côte d'ivoire. | enteropathogenic yersinia circulate in the pig reservoir and are the third bacterial cause of human gastrointestinal infections in europe. in west africa, reports of human yersiniosis are rare. this study was conducted to determine whether pathogenic yersinia are circulating in pig farms and are responsible for human infections in the abidjan district. | 2017 | 28081123 |
| virulence-related genes, adhesion and invasion of some yersinia enterocolitica-like strains suggests its pathogenic potential. | yersina enterocolitica-like species have not been extensively studied regarding its pathogenic potential. this work aimed to assess the pathogenic potential of some y. enterocolitica-like strains by evaluating the presence of virulence-related genes by pcr and their ability to adhere to and invade caco-2 and hep-2 cells. a total of 50 y. frederiksenii, 55 y. intermedia and 13 y. kristensenii strains were studied. the strains contained the following genes: y. frederiksenii, fepa(44%), fes(44%) an ... | 2017 | 28062290 |
| sepsis and siderosis, yersinia enterocolitica and hereditary haemochromatosis. | a 60-year-old woman was admitted with sepsis, relative bradycardia, ct evidence of numerous small liver abscesses and 'skin bronzing' consistent with hereditary haemochromatosis (hh). yersinia enterocolitica o:9 infection was confirmed by serology specimens taken 10 days apart. iron overload was detected, and homozygous c282y gene mutation confirmed hh. liver biopsy revealed grade iv siderosis with micronodular cirrhosis. haemochromatosis is a common, inherited disorder leading to iron overload ... | 2017 | 28052950 |
| mechanisms of yersinia yopo kinase substrate specificity. | yersinia bacteria cause a range of human diseases, including yersiniosis, far east scarlet-like fever and the plague. yersiniae modulate and evade host immune defences through injection of yersinia outer proteins (yops) into phagocytic cells. one of the yops, yopo (also known as ypka) obstructs phagocytosis through disrupting actin filament regulation processes - inhibiting polymerization-promoting signaling through sequestration of rac/rho family gtpases and by using monomeric actin as bait to ... | 2017 | 28051168 |
| several hfq-dependent alterations in physiology of yersinia enterocolitica o:3 are mediated by derepression of the transcriptional regulator rovm. | in bacteria, the rna chaperone hfq enables pairing of small regulatory rnas with their target mrnas and therefore is a key player of post-transcriptional regulation network. as a global regulator, hfq is engaged in the adaptation to external environment, regulation of metabolism and bacterial virulence. in this study we used rna-sequencing and quantitative proteomics (lc-ms/ms) to elucidate the role of this chaperone in the physiology and virulence of yersinia enterocolitica serotype o:3. this g ... | 2017 | 28010054 |
| genomic insights and its comparative analysis with yersinia enterocolitica reveals the potential virulence determinants and further pathogenicity for foodborne outbreaks. | yersinia enterocolitica is a well-known foodborne pathogen causing gastrointestinal infections worldwide. the strain y. enterocolitica forc_002 was isolated from the gill of flatfish (plaice) and its genome was sequenced. the genomic dna consists of 4,837,317 bp with a gc content of 47.1%, and is predicted to contain 4,221 open reading frames, 81 trna genes, and 26 rrna genes. interestingly, genomic analysis revealed pathogenesis and host immune evasion-associated genes encoding guanylate cyclas ... | 2017 | 27974737 |
| sheep carrying pathogenic yersinia enterocolitica bioserotypes 2/o:9 and 5/o:3 in the feces at slaughter. | yersinia enterocolitica is a heterogeneous species including non-pathogenic strains belonging to biotype 1a and pathogenic strains belonging to biotypes 1b and 2-5. pathogenic strains of biotypes 2-4 carrying the ail virulence gene have frequently been isolated from domestic pigs at slaughter. in sheep, mostly non-pathogenic biotype 1a strains have been reported. in our study, the prevalence of ail-positive y. enterocolitica was studied by pcr and culturing in 406 young sheep (<1year of age) and ... | 2016 | 27938687 |