| detection of major diarrheagenic bacterial pathogens by multiplex pcr panels. | diarrheal diseases remain a major threat to the youngest population in low- and middle-income countries. the main bacterial pathogens causing diarrhea are diarrheagenic escherichia coli (dec) that consists of enteroaggregative (eaec), enteropathogenic (epec), enterotoxigenic (etec), enterohemorrhagic ehec and enteroinvasive e. coli (eiec), salmonella, shigella spp. (s. dysenteria, s. sonnei, s. flexneri) campylobacter (c. coli, c. jejuni), vibrio (v. vulnificus, v. parahaemolyticusm, v. cholerae ... | 2015 | 25542594 |
| antimicrobial efficacy and in vivo toxicity studies of a quaternized biopolymeric flocculant. | this study evaluated the antibacterial spectrum and safety of a chemically modified biopolymeric flocculant (tmb) against waterborne pathogens. the biopolymer previously characterized as polysaccharide with flocculating activity is produced extracellularly by the bacterium klebsiella terrigena. the amino sugars on the polymer were chemically modified by quaternization, which resulted in n,n,n trimethyl biopolymer (tmb). quaternization was effective in imparting biocidal activity to tmb against f ... | 2014 | 25473973 |
| [sensitivity of three inmunocromathographic tests in faeces samples for campylobacter and salmonella detection in comparison to culture]. | introduction: campylobacter sp. and salmonella enterica are two of the main organisms causing gastroenteritis in our environment. immunochromatographic tests for antigen detection performed directly on stool samples for its simplicity and rapid results may make them useful diagnostic elements in the context of primary care. | 2014 | 24940890 |
| probiotic characterization of potential hydrolases producing lactococcus lactis subsp. lactis isolated from pickled yam. | the aim of this study was to characterize potential probiotic strain co-producing α-amylase and β-galactosidase. sixty-three strains, isolated from pickle samples were screened for their hydrolase producing capacity by utilizing different starches as carbon source. one out of 63 strains, isolated from traditionally fermented pickled yam showing maximum hydrolase activity (α-amylase (36.9 u/ml) and β-galactosidase (42.6 u/ml)) within a period of 48 hours was identified as lactococcus lactis subsp ... | 2014 | 24020495 |
| development of a quaternized chitosan with enhanced antibacterial efficacy. | the antibacterial activity of a water-soluble chitosan derivative prepared by chemical modification to quaternary ammonium compound n,n,n-trimethylchitosan (tc) was investigated against four selected waterborne pathogens: aeromonas hydrophila atcc 35654, yersinia enterocolitica atcc 9610, listeria monocytogenes atcc 19111 and escherichia coli o157:h7 atcc 32150. an inactivation of 4 log cfu/ml of all waterborne pathogens was noted for the quaternized chitosan as compared with chitosan over a sho ... | 2013 | 23981870 |
| emergence and stability of high-pressure resistance in different food-borne pathogens. | high hydrostatic pressure (hhp) processing is becoming a valuable nonthermal food pasteurization technique, although there is reasonable concern that bacterial hhp resistance could compromise the safety and stability of hhp-processed foods. while the degree of natural hhp resistance has already been shown to vary greatly among and within bacterial species, a still unresolved question remains as to what extent different food-borne pathogens can actually develop hhp resistance. in this study, we t ... | 2012 | 22344661 |
| [correlation between maldi-tof vitek-mstm system and conventional identification methods of gastrointestinal infection causing bacteria]. | rapid identification of pathogens is essential for the diagnosis of gastrointestinal infections. matrix-assisted laser desorption/ionization time of flight (maldi-tof) mass spectrometry has shown to be effective and fast for the identification of microorganisms. the objective of this study was to evaluate the correlation between vitek-mstm and conventional methods for bacterial identification causing gastrointestinal infection. | 2016 | 27454012 |
| [infectious etiology of acute idiopathic intussusception in children]. | the intussusception (iia) is an invagination of the immediate part of the intestine. she is responsible for a syndrome with an occlusive venous compression and swelling that can rapidly progress to intestinal necrosis. most cases occur in children aged 6 to 18 months and occur more frequently in boys than girls. there are two types of iia: the iia idiopathic representing 90-95% of invaginations of the child and the iia secondary to local injury of the gastrointestinal tract or occurring in a par ... | 2013 | 23906565 |
| cluster analysis of host cytokine responses to biodefense pathogens in a whole blood ex vivo exposure model (weem). | rapid detection and therapeutic intervention for infectious and emerging diseases is a major scientific goal in biodefense and public health. toward this end, cytokine profiles in human blood were investigated using a human whole blood ex vivo exposure model, called weem. | 2012 | 22607329 |
| cytotoxicity, bactericidal, and antioxidant activity of sodium alginate hydrosols treated with direct electric current. | the aim of the study was to investigate the effect of using direct electric current (dc) of 0, 200, and 400 ma for five minutes on the physiochemical properties, cytotoxicity, antibacterial, and antioxidant activity of sodium alginate hydrosols with different sodium chloride concentrations. the ph, oxidation-reduction potential (orp), electrical conductivity (ec), and available chlorine concentration (acc) were measured. the effect of sodium alginate hydrosols treated with dc on staphylococcus a ... | 2017 | 28327520 |
| in vitro evaluation of the antimicrobial ability and cytotoxicity on two melanoma cell lines of a benzylamide derivative of maslinic acid. | maslinic acid is a pentacyclic triterpene extracted from olives that has been systematically reported to exert several therapeutic effects, such as antitumoral, antidiabetic, antioxidant, anti-inflammatory, antiparasitic, and antiviral properties. recently, new derivatives of maslinic acid have been obtained and expanded the spectrum of biological activities and improved the existing ones. the present study was meant to perform the in vitro assessment of the (i) cytotoxic effects of a benzylamid ... | 2016 | 28050335 |
| differences in chemical, physical and microbiological characteristics of italian burrata cheeses made in artisanal and industrial plants of apulia region. | the burrata cheese is a traditional product from southern italy, consisting of an envelope of pasta filata (stretched curd) filled with cream and pasta filata strips (usually leftovers from mozzarella production). physical [water activity (aw), ph], chemical (moisture, nacl content) and microbiological [total viable count (tvc), listeria monocytogenes, salmonella spp., yersinia enterocolitica, bacillus cereus, escherichia coli, enterobacteriaceae, coagulase-positive staphylococci] characteristic ... | 2016 | 27853715 |
| simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods. | capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ... | 2016 | 27494415 |
| the impact of oxygen on bacterial enteric pathogens. | bacterial enteric pathogens are responsible for a tremendous amount of foodborne illnesses every year through the consumption of contaminated food products. during their transit from contaminated food sources to the host gastrointestinal tract, these pathogens are exposed and must adapt to fluctuating oxygen levels to successfully colonize the host and cause diseases. however, the majority of enteric infection research has been conducted under aerobic conditions. to raise awareness of the import ... | 2016 | 27261784 |
| hygienic characteristics and microbiological hazard identification in horse and donkey raw milk. | today the interest toward horse (equus caballus) and donkey (equus asinus) milk for human consumption is receiving a renewed attention because of its particular composition, hypoallergenicity, and nutraceutical properties. the realistic perspective of global use of this aliment in balanced diets, especially for infancy and geriatrics, poses the need for a more in depth knowledge on milk hygiene and on the health status of dairy animals, as a prerequisite of consumers' safety. the aim of this pap ... | 2017 | 27033528 |
| enteric bacteria isolated from diarrheal patients in korea in 2014. | the aim of this study was to characterize the pathogens responsible for causing diarrhea according to season, region of isolation, patient age, and sex as well as to provide useful data for the prevention of diarrheal disease. | 2015 | 26473090 |
| supercritical co2 extraction of functional compounds from spirulina and their biological activity. | supercritical carbon dioxide (scco2) extraction and fractionation of spirulina platensis was carried out to obtain functional compounds with antioxidant, antimicrobial and enzyme inhibitory activities. extraction of scco2 was carried out using 200 g of spirulina powder at 40 ºc under 120 bar pressure with co2 flow rate of 1.2 kg h(-1). scco2 fraction obtained was further treated with hexane and ethyl acetate to identify its components. individual components were identified by comparing mass spec ... | 2015 | 26028745 |
| synthesis and antibacterial properties of 2,3-dideoxyglucosides of terpene alcohols and phenols. | essential oils and their oxygenated terpene constituents possess potent antimicrobial properties. in the present study, a facile synthetic route to the 2,3-dideoxy 1-o-glucosides of important phenols and terpene alcohols in excellent yields (85-96%) has been delineated. studies on their antimicrobial action against four food-borne pathogens--bacillus cereus, staphylococcus aureus, escherichia coli and yersinia enterocolitica--demonstrated that the zone of inhibition, in general, was higher for t ... | 2015 | 25952857 |
| microbiological diversity and prevalence of spoilage and pathogenic bacteria in commercial fermented alcoholic beverages (beer, fruit wine, refined rice wine, and yakju). | the present study examined 469 commercially available fermented alcoholic beverages (fabs), including beer (draft, microbrewed, and pasteurized), fruit wine (grape and others), refined rice wine, and yakju (raw and pasteurized). samples were screened for escherichia coli and eight foodborne pathogens (bacillus cereus, campylobacter jejuni, clostridium perfringens, escherichia coli o157:h7, listeria monocytogenes, salmonella spp., staphylococcus aureus, and yersinia enterocolitica), and the aerob ... | 2015 | 25836410 |
| expert elicitation as a means to attribute 28 enteric pathogens to foodborne, waterborne, animal contact, and person-to-person transmission routes in canada. | enteric illness contributes to a significant burden of illness in canada and globally. understanding its sources is a critical step in identifying and preventing health risks. expert elicitation is a powerful tool, used previously, to obtain information about enteric illness source attribution where information is difficult or expensive to obtain. thirty-one experts estimated transmission of 28 pathogens via major transmission routes (foodborne, waterborne, animal contact, person-to-person, and ... | 2015 | 25835810 |
| pyrosequencing analysis of microbial community and food-borne bacteria on restaurant cutting boards collected in seri kembangan, malaysia, and their correlation with grades of food premises. | this study adopts the pyrosequencing technique to identify bacteria present on 26 kitchen cutting boards collected from different grades of food premises around seri kembangan, a city in malaysia. pyrosequencing generated 452,401 of total reads of otus with an average of 1.4×10(7) bacterial cells/cm(2). proteobacteria, firmicutes and bacteroides were identified as the most abundant phyla in the samples. taxonomic richness was generally high with >1000 operational taxonomic units (otus) observed ... | 2015 | 25679309 |
| effects of climate change on the persistence and dispersal of foodborne bacterial pathogens in the outdoor environment: a review. | according to the intergovernmental panel on climate change (ipcc), warming of the climate system is unequivocal. over the coming century, warming trends such as increased duration and frequency of heat waves and hot extremes are expected in some areas, as well as increased intensity of some storm systems. climate-induced trends will impact the persistence and dispersal of foodborne pathogens in myriad ways, especially for environmentally ubiquitous and/or zoonotic microorganisms. animal hosts of ... | 2016 | 25612827 |
| synthesis, characterization and antibacterial activity of biodegradable films prepared from schiff bases of zein. | pure zein is known to be very hydrophobic, but is still inappropriate for coating and film applications because of their brittle nature. in an attempt to improve the flexibility and the antimicrobial activity of these coatings and films, chemical modification of zein through forming schiff bases with different phenolic aldhydes was tried. influence of this modifications on mechanical, topographical, wetting properties and antimicrobial activity of zein films were evaluated. the chemical structur ... | 2014 | 25328181 |
| prevalence and characterization of foodborne pathogens from australian dairy farm environments. | the ability of foodborne pathogens to gain entry into food supply systems remains an ongoing concern. in dairy products, raw milk acts as a major vehicle for this transfer; however, the sources of pathogenic bacteria that contaminate raw milk are often not clear, and environmental sources of contamination or the animals themselves may contribute to the transfer. this survey examined the occurrence of 9 foodborne pathogens in raw milk and environments of 7 dairy farms (3 bovine, 3 caprine, and 1 ... | 2014 | 25282417 |
| antibacterial, antiviral, and antifungal properties of wines and winery byproducts in relation to their flavonoid content. | grapes produce organic compounds that may be involved in the defense of the plants against invading phytopathogens. these metabolites include numerous phenolic compounds that are also active against human pathogens. grapes are used to produce a variety of wines, grape juices, and raisins. grape pomace, seeds, and skins, the remains of the grapes that are a byproduct of winemaking, also contain numerous bioactive compounds that differ from those found in grapes and wines. this overview surveys an ... | 2014 | 24945318 |
| toxin production and growth of pathogens subjected to temperature fluctuations simulating consumer handling of cold cuts. | it is crucial for the quality and safety of ready-to-eat (rte) foods to maintain the cold chain from production to consumption. the effect of temperature abuse related to daily meals and elevated refrigerator temperatures on the growth and toxin production of bacillus cereus, bacillus weihenstephanensis and staphylococcus aureus and the growth of listeria monocytogenes and yersinia enterocolitica was studied. a case study with temperature loggings in the domestic environment during easter and ch ... | 2014 | 24935689 |
| development of 23 individual taqman® real-time pcr assays for identifying common foodborne pathogens using a single set of amplification conditions. | most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. the aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. the list of targets include, among the others, listeria spp., salmonella spp., shigella spp., escherichia coli spp., campylobacter spp., clostridium spp. and staphylococcus aureus. the accuracy of detection was deter ... | 2014 | 24929880 |
| food-borne pathogens of animal origin-diagnosis, prevention, control and their zoonotic significance: a review. | the term food borne diseases or food-borne illnesses or more commonly food poisoning are used to denote gastrointestinal complications that occur following recent consumption of a particular food or drink. millions of people suffer worldwide every year and the situation is quiet grave in developing nations creating social and economic strain. the food borne pathogens include various bacteria viz., salmonella, campylobacter, escherichia coli, listeria monocytogenes, yersinia enterocolitica, staph ... | 2013 | 24506006 |
| yaxab, a yersinia enterocolitica pore-forming toxin regulated by rova. | the transcriptional regulator rova positively regulates transcription of the yersinia enterocolitica virulence gene inv. invasin, encoded by inv, is important for establishment of y. enterocolitica infection. however, a rova mutant is more attenuated for virulence than an inv mutant, implying that rova regulates additional virulence genes. when the y. enterocolitica rova regulon was defined by microarray analysis, ye1984 and ye1985 were among the genes identified as being upregulated by rova. si ... | 2013 | 24002058 |
| antibacterial and antimutagenic activities of novel zerumbone analogues. | zerumbone, the key constituent of zingiber zerumbet smith, is a very important bioactive phytochemical. two new compounds viz. azazerumbone 1 and azazerumbone 2 were synthesised by zncl2-catalysed beckmann rearrangement of the zerumbone oxime. the structure elucidation of these analogues of zerumbone was carried out by 1d ((1)h nmr and (13)c nmr) and 2d-nmr (cosy, hsqc and noesy) spectral analysis. studies on the antibacterial activity established that azazerumbone 2 had better activity than zer ... | 2013 | 23790891 |
| causes of infectious acute diarrhea in infants treated at pediatric clinic. | the aim of this work has been a presentation of causes of acute infectious diarrhea. | 2013 | 23678831 |
| effect of the temperature of the dipping solution on the antimicrobial effectiveness of various chemical decontaminants against pathogenic and spoilage bacteria on poultry. | the influence of the temperature of the dipping solution on the antimicrobial effectiveness of several chemical poultry decontaminants was assessed. a total of 765 poultry legs were inoculated with gram-positive bacteria (listeria monocytogenes, staphylococcus aureus, bacillus cereus, or brochothrix thermosphacta) or gram-negative bacteria (salmonella enterica serotype enteritidis, escherichia coli, yersinia enterocolitica, or pseudomonas fluorescens). samples were dipped for 15 min in solutions ... | 2013 | 23643125 |
| synthesis of amino acid conjugates of tetrahydrocurcumin and evaluation of their antibacterial and anti-mutagenic properties. | tetrahydrocurcumin (thc), the hydrogenated and stable form of curcumin, exhibits physiological and pharmacological activities similar to curcumin. a protocol has been developed for the synthesis of novel conjugates of thc with alanine (2a), isoleucine (2b), proline (2c), valine (2d), phenylalanine (2e), glycine (2f) and leucine (2g) in high yields (43-82%). all the derivatives of thc exhibited more potent anti-microbial activity than thc against bacillus cereus, staphylococcus aureus, escherichi ... | 2013 | 23561114 |
| pathogenic bacteria in finnish bulk tank milk. | while the quality of raw cow milk in finland is known for its high hygienic standard, with the national average total bacterial count being below 10(4) cfu/ml annually, the prevalence of pathogenic bacteria in finnish raw milk is underreported. the aim of this study was to determine the occurrence of listeria monocytogenes, thermophilic campylobacter spp., salmonella spp., stx-positive escherichia coli (stec), coagulase-positive staphylococci, yersinia spp., and bacillus cereus group in raw cow ... | 2013 | 23373473 |
| changes in microbial contamination levels of porcine carcasses and fresh pork in slaughterhouses, processing lines, retail outlets, and local markets by commercial distribution. | the objective of this study was to evaluate the changes in microbial contamination levels of each porcine carcass and fresh pork in a general distribution process. a total of 100 commercial pigs were sampled (six sampling sites per individual, total 600 samples) at four sequential stages: slaughterhouse (after carcass grading and boning), processing line, retail outlet, and local market. no significant differences were observed in the contaminant percentages among sampling sites and sample colle ... | 2013 | 23273786 |
| synthesis of quinoline derivatives of tetrahydrocurcumin and zingerone and evaluation of their antioxidant and antibacterial attributes. | tetrahydrocurcumin (thc, 1) and zingerone (2) are biologically active molecules originating from the important spices turmeric and ginger, respectively. novel quinoline derivatives of thc and zingerone have been synthesised by an efficient protocol involving their reaction with substituted 2-aminobenzophenones and 2-aminoacetophenone. radical-scavenging activities (rsa) of thc, zingerone and their quinoline derivatives were evaluated. the amino-substituted quinoline derivative of thc, 1e, showed ... | 2013 | 23122110 |
| rapid detection and respirometric profiling of aerobic bacteria on panels of selective media. | to evaluate high-throughput optical oxygen microrespirometry for selective detection and predictive identification of aerobic bacteria. | 2013 | 23107004 |
| prevalence of foodborne pathogens in retailed foods in thailand. | the consumption of foodborne pathogens contaminated in food is one of the major causes of diarrheal diseases in thailand. the objective of this study was to evaluate the prevalence and types of contaminating bacteria in retailed foodstuffs in thailand. food from four categories (137 samples total), including meat (51 samples), vegetables (38 samples), fish or seafood (37 samples), and fermented food (11 samples), was purchased randomly from seven different open-markets and seven supermarkets in ... | 2012 | 22953752 |
| optimizing the production of the biosurfactant lichenysin and its application in biofilm control. | apply response surface methodology (rsm) to develop and optimize an economical medium for lichenysin production, which is a surfactant produced by bacillus licheniformis and evaluate the application of lichenysin in the prevention and disruption of pathogenic micro-organism biofilm that creates health problems in the food industry and hospitals. | 2016 | 26519210 |
| empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria. | in this study, fractional compound composition, antioxidant capacity, and phenolic substance content of 14 plant essential oils-anise (pimpinella anisum), bay leaves (laurus nobilis), cinnamon bark (cinnamomum verum), clove (eugenia caryophyllata), fennel (foeniculum vulgare), hop (humulus lupulus), istanbul oregano (origanum vulgare subsp. hirtum), izmir oregano (origanum onites), mint (mentha piperita), myrtus (myrtus communis), orange peel (citrus sinensis), sage (salvia officinalis), thyme ( ... | 2015 | 25764982 |
| conventional and molecular methods in the diagnosis of community-acquired diarrhoea in children under 5 years of age from the north-eastern region of poland. | the purpose of this study was to determine the main causative agents of community-acquired acute diarrhoea in children using conventional methods and pcr. | 2015 | 26159845 |
| plasmacytoid dendritic cells are crucial in bifidobacterium adolescentis-mediated inhibition of yersinia enterocolitica infection. | in industrialized countries bacterial intestinal infections are commonly caused by enteropathogenic enterobacteriaceae. the interaction of the microbiota with the host immune system determines the adequacy of an appropriate response against pathogens. in this study we addressed whether the probiotic bifidobacterium adolescentis is protective during intestinal yersinia enterocolitica infection. female c57bl/6 mice were fed with b. adolescentis, infected with yersinia enterocolitica, or b. adolesc ... | 2013 | 23977019 |
| antagonistic activities of some bifidobacterium sp. strains isolated from resident infant gastrointestinal microbiota on gram-negative enteric pathogens. | the gastrointestinal microbiota contributes to the consolidation of the anti-infectious barrier against enteric pathogens. the purpose of this study was to investigate the influence of bifidobacterium sp. strains, recently isolated from infant gastrointestinal microbiota on the in vitro growth and virulence features expression of enteropathogenic bacterial strains. the antibacterial activity of twelve bifidobacterium sp. strains isolated from human feces was examined in vitro against a wide rang ... | 2016 | 26921694 |
| review of bacterial and viral zoonotic infections transmitted by dogs. | dogs are a major reservoir for zoonotic infections. dogs transmit several viral and bacterial diseases to humans. zoonotic diseases can be transmitted to human by infected saliva, aerosols, contaminated urine or feces and direct contact with the dog. viral infections such as rabies and norovirus and bacterial infections including pasteurella, salmonella, brucella, yersinia enterocolitica, campylobacter, capnocytophaga, bordetella bronchiseptica, coxiella burnetii, leptospira, staphylococcus inte ... | 2015 | 28316698 |
| seroprevalence of campylobacteriosis and relevant post-infectious sequelae. | post-infectious sequelea such as guillain barré syndrome (gbs), reactive arthritis (ra), and inflammatory bowel disease (ibd) may arise as a consequence of acute campylobacter-enteritis (ae). however, reliable seroprevalence data of campylobacter-associated sequelae has not been established. the objectives of this study were, first, to identify the most specific and sensitive test antigen in an optimized elisa assay for diagnosing a previous campylobacter-infection and, second, to compare the pr ... | 2014 | 24413899 |
| occurrence of pathogens in wild rodents caught on swedish pig and chicken farms. | a total of 207 wild rodents were caught on nine pig farms, five chicken farms and five non-farm locations in sweden and surveyed for a selection of bacteria, parasites and viruses. lawsonia intracellularia and pathogenic yersinia enterocolitica were only detected in rodents on pig farms (9% and 8% prevalence, respectively) which indicate that these agents are more likely to be transmitted to rodents from pigs or the environment on infected farms. brachyspira hyodysenteriae (1%), brachyspira inte ... | 2013 | 23174339 |
| an evaluation of elisa using recombinant brucella abortus bacterioferritin (bfr) for bovine brucellosis. | to date, detection of antibodies against the lipopolysaccharide portion is the backbone of most serodiagnostic methods for brucellosis screening. however this pose a risk for false positive reactions related to other pathogens especially that of yersinia enterocolitica o:9 which has the most prominent cross reactivity with brucella spp. in this study, cloning and expression of brucella abortus bacterioferritin (bfr) was accomplished by pcr amplification into an expression vector system, and puri ... | 2016 | 27012915 |
| a review of the basis of the immunological diagnosis of ruminant brucellosis. | bacteria of the genus brucella cause brucellosis, the most common bacterial zoonosis worldwide. the diagnosis of brucella abortus and brucella melitensis ruminant brucellosis is based on bacteriological and immunological tests, the latter being routinely used in control and eradication and surveillance programs. infections by smooth and rough brucella spp., the use of smooth and rough vaccines, and the false-positive serological reactions caused by yersinia enterocolitica o:9 and other cross-rea ... | 2016 | 26964721 |
| serological diagnosis of bovine brucellosis using b. melitensis strain b115. | bovine brucellosis is diagnosed by official tests, such as rose bengal plate test (rbpt) and complement fixation test (cft). both tests detect antibodies directed against the lipolysaccharide (lps) of brucella cell wall. despite their good sensitivity, those tests do not discriminate between true positive and false positive serological reactions (fpsr), the latter being generated by animals infected with other gram negative microorganisms that share components of brucella lps. in this study, an ... | 2015 | 26476137 |
| proteomic analyses of the time course responses of mice infected with brucella abortus 544 reveal immunogenic antigens. | brucellosis is a major zoonotic disease caused by pathogens of the genus brucella. the eradication of brucellosis in domestic animals, associated with the prevention of human infection, can be attained through accurate diagnosis. however, the conventional serological diagnosis of brucellosis has limitations, particularly in detecting the infection period. accordingly, the aim of this study was to determine reliable immunogenic proteins to detect brucella abortus infection according to time cours ... | 2014 | 24975114 |
| immunoproteomics of brucella abortus rb51 as candidate antigens in serological diagnosis of brucellosis. | the current brucellosis serodiagnostic assays are chiefly based on detecting anti-lps (lipopolysaccharide) antibodies. however, cross-reaction with some gram-negative bacteria can occasionally induce due to similar o-polysaccharide (ops) structure. therefore, the aim of the present study was to identify new candidate antigens from brucella abortus rb51, a mutant strain lacking the lps portion, which might be valuable in brucellosis diagnosis. to detect potential antigens, immobilized ph gradient ... | 2014 | 24908638 |
| yersinia enterocolitica: an unlikely cause of positive brucellosis tests in greater yellowstone ecosystem bison (bison bison). | yersinia enterocolitica serotype o:9 has identical o-antigens to those of brucella abortus and has apparently caused false-positive reactions in numerous brucellosis serologic tests in elk (cervus canadensis) from southwest montana. we investigated whether a similar phenomenon was occurring in brucellosis antibody-positive bison (bison bison) using y. enterocolitica culturing techniques and multiplex pcr of four diagnostic loci. feces from 53 yellowstone bison culled from the population and 113 ... | 2012 | 22740518 |
| serological diagnostic potential of recombinant outer membrane proteins (romps) from brucella melitensis in mouse model using indirect enzyme-linked immunosorbent assay. | brucella melitensis is the most important pathogenic species of brucella spp. which affects goats and sheep and causes caprine and ovine brucellosis, respectively. serological tests for diagnosis of brucellosis such as rose bengal plate test (rbpt) and enzyme-linked immunosorbent assay (elisa) usually utilize smooth lipopolysaccharides (s-lps) as a diagnostic antigen which could give false positive serological reactions. outer membrane proteins (omp) of b. melitensis have been used as alternativ ... | 2015 | 26530141 |
| [identification of zoonotic bacterial pathogens by the maldi tof ms method]. | to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work. | 2015 | 26312374 |
| performance of skin tests with allergens from b. melitensis b115 and rough b. abortus mutants for diagnosing swine brucellosis. | swine brucellosis by brucella suis biovar 2 is an emerging disease whose control is based on serological testing and culling. however, current serological tests detect antibodies to the o-polysaccharide (o/ps) moiety of brucella smooth lipopolysaccharide (s-lps), and thus lack specificity when infections by yersinia enterocolitica o:9 and other gram-negative bacteria carrying cross-reacting o/ps occur. the skin test with the protein-rich brucellin extract obtained from rough b. melitensis b115 i ... | 2014 | 24331743 |
| the epitopic and structural characterization of brucella suis biovar 2 o-polysaccharide demonstrates the existence of a new m-negative c-negative smooth brucella serovar. | the brucellae are gram-negative bacteria that cause an important zoonosis. studies with the main brucella species have shown that the o-antigens of the brucella smooth lipopolysaccharide are α-(1 → 2) and α-(1 → 3)-linked n-formyl-perosamine polysaccharides that carry m, a and c (a = m, a>m and a<m) epitopes relevant in serodiagnosis and typing. we report that, in contrast to the b. suis biovar 1 o-antigen used as a reference or to all described brucella o-antigens, b. suis biovar 2 o-antigen fa ... | 2013 | 23335981 |
| an evaluation of the capability of existing and novel serodiagnostic methods for porcine brucellosis to reduce false positive serological reactions. | porcine brucellosis is a zoonotic disease of truly global significance because even in countries without the disease the occurrence of false positive serological reactions (fpsrs) creates significant problems. statutory diagnostic testing is required in many disease free countries or regions and is often a prerequisite for the movement of live animals. currently this testing is dependent almost entirely on serological assays and these may result in a significant number of fpsrs. the aim of this ... | 2012 | 22763172 |
| zoonotic public health hazards in backyard chickens. | backyard poultry has become increasingly popular in industrialized countries. in addition to keeping chickens for eggs and meat, owners often treat the birds as pets. however, several pathogenic enteric bacteria have the potential for zoonotic transmission from poultry to humans but very little is known about the occurrence of zoonotic pathogens in backyard flocks. the occurrence and the antimicrobial resistance of salmonella enterica, campylobacter spp., listeria monocytogenes and enteropathoge ... | 2016 | 26752227 |
| specific pathogen-free pig herds also free from campylobacter? | as specific pathogen-free (spf) pig herds are designed and managed to prevent specific pig diseases, it might be feasible to expand the list of micro-organisms also including zoonotic pathogens such as campylobacter coli as this agent has its origin in pigs. in a previous survey, 15 of 16 of spf herds were found free from human pathogenic yersinia enterocolitica. accordingly, three nucleus and seven multiplying herds were surveyed for campylobacter to investigate whether the norwegian spf pig py ... | 2015 | 24798507 |
| effects of lactic acid and commercial chilling processes on survival of salmonella, yersinia enterocolitica, and campylobacter coli in pork variety meats. | current industry chilling practices with and without the application of 2% l-lactic acid were compared for their effectiveness at reducing levels of salmonella, yersinia enterocolitica, and campylobacter coli on pork variety meats. pork variety meats (livers, intestines, hearts, and stomachs) were inoculated individually with one of the three pathogens and subjected to five different treatment combinations that included one or more of the following: water wash (25°c), lactic acid spray (2%, 40 t ... | 2012 | 22947465 |
| antimicrobial use for and resistance of zoonotic bacteria recovered from nonhuman primates. | as a growing threat to human and animal health, antimicrobial resistance (amr) has become a central public-health topic. largescale surveillance systems, such as the national antimicrobial resistance monitoring system (narms), are now established to monitor and provide guidance regarding amr, but comprehensive literature on amr among nhp is sparse. this study provides data regarding current antimicrobial use strategies and the prevalence of amr in zoonotic bacteria recovered from nhp within biom ... | 2017 | 28222842 |
| antimicrobial use for and resistance of zoonotic bacteria recovered from nonhuman primates. | as a growing threat to human and animal health, antimicrobial resistance (amr) has become a central public-health topic. largescale surveillance systems, such as the national antimicrobial resistance monitoring system (narms), are now established to monitor and provide guidance regarding amr, but comprehensive literature on amr among nhp is sparse. this study provides data regarding current antimicrobial use strategies and the prevalence of amr in zoonotic bacteria recovered from nhp within biom ... | 2017 | 28128070 |
| a screening algorithm for diagnosing bacterial gastroenteritis by real-time pcr in combination with guided culture. | we have introduced a real-time pcr for the simultaneous detection of campylobacter jejuni, salmonella spp., shigella spp./enteroinvasive escherichia coli and yersinia enterocolitica in fecal samples in our routine laboratory. this new approach showed consistent results, with minimal inter-sample variation. when compared to conventional culture, the hands-on time decreased by 13 h/wk, and the median turnaround time drastically shortened from 73 to 29 h (p < .0001). moreover, the detection rate of ... | 2016 | 27107537 |
| susceptibility of bacteria isolated from acute gastrointestinal infections to rifaximin and other antimicrobial agents in mexico. | bacterial resistance may hamper the antimicrobial management of acute gastroenteritis. bacterial susceptibility to rifaximin, an antibiotic that achieves high fecal concentrations (up to 8,000μg/g), has not been evaluated in mexico. | 2017 | 26525276 |
| a probe-free four-tube real-time pcr assay for simultaneous detection of twelve enteric viruses and bacteria. | we aim to develop a multiplex real-time pcr assay to detect the most common pathogens causing community outbreaks of diarrhea. | 2015 | 26342434 |
| reactive arthritis in relation to internal derangements of the temporomandibular joint: a case control study. | the aim of this study was to find out if reactive arthritis was involved in the aetiology of chronic closed lock of the temporomandibular joint (tmj) by looking for bacterial antigens in the synovial membrane of the tmj, and by studying the antibody serology and carriage of human leucocyte antigen (hla) b27 in patients with chronic closed lock. patients with reciprocal clicking and healthy subjects acted as controls. we studied a total of 43 consecutive patients, 15 with chronic closed lock, 13 ... | 2015 | 25957137 |
| evaluation of a real-time multiplex pcr for the simultaneous detection of campylobacter jejuni, salmonella spp., shigella spp./eiec, and yersinia enterocolitica in fecal samples. | conventional diagnosis of infectious diarrhea caused by bacteria is time-consuming, labor-intensive, and has a suboptimal sensitivity. we have therefore developed a multiplex real-time polymerase chain reaction (pcr) for the simultaneous detection of campylobacter jejuni, salmonella spp., shigella spp./enteroinvasive escherichia coli (eiec), and yersinia enterocolitica in fecal samples. no cross reactivity between the different pathogens was observed, and the multiplex setup of the assay did not ... | 2015 | 25326870 |
| [ill after drinking untreated milk; 'die amelander krankheit']. | in 2012 three patients consulted their general practitioner with symptoms of gastro-enteritis with bloody stools. this was caused by drinking untreated milk infected with campylobacter jejuni. another patient developed reactive arthritis. he too had drunk untreated milk that had probably been infected with yersinia enterocolitica. between 1958 and 1995 many german children living in the region of cologne developed gastro-enteritis after holidaying on ameland, one of the dutch islands. this condi ... | 2013 | 24345367 |
| drinking water for dairy cattle: always a benefit or a microbiological risk? | drinking water can be considered an essential nutrient for dairy cattle. however, because it comes from different sources, its chemical and microbiological quality does not always reach accepted standards. moreover, water quality is not routinely assessed on dairy farms. the microecology of drinking water sources and distribution systems is rather complex and still not fully understood. water quality is adversely affected by the formation of biofilms in distribution systems, which form a persist ... | 2013 | 23457917 |
| first reported case of campylobacter lanienae enteritis in a human. | campylobacters are the most frequently identified bacteria causing diarrhoea in humans worldwide. campylobacter lanienae was isolated for the first time in 2000 from faecal samples of two asymptomatic abattoir workers in switzerland during a routine hygiene screen, but has never been associated with human disease. | 2016 | 28348764 |
| a study on antimicrobial, antioxidant and antimutagenic activities of elaeagnus angustifolia l. leaves. | [corrected] the aim of this work was to investigate the antimicrobial, antioxidant, and antimutagenic potentials of methanol extracts from e. angustifolia. | 2014 | 24653563 |
| preparation and antimicrobial action of three tryptic digested functional molecules of bovine lactoferrin. | lactoferrin is an 80 kda bilobal, iron binding glycoprotein which is primarily antimicrobial in nature. the hydrolysis of lactoferrin by various proteases in the gut produces several functional fragments of lactoferrin which have varying molecular sizes and properties. here, bovine lactoferrin has been hydrolyzed by trypsin, the major enzyme present in the gut, to produce three functional molecules of sizes approximately 21 kda, 38 kda and 45 kda. the molecules have been purified using ion excha ... | 2014 | 24595088 |
| the extended structure of the periplasmic region of cdsd, a structural protein of the type iii secretion system of chlamydia trachomatis. | the type iii secretion system (t3ss) is required for the virulence of many gram-negative bacterial human pathogens. it is composed of several structural proteins, forming the secretion needle and its basis, the basal body. in chlamydia spp., the t3ss inner membrane ring (im-ring) of the basal body is formed by the periplasmic part of cdsd (outer ring) and cdsj (inner ring). here we describe the crystal structure of the c-terminal, periplasmic part of cdsd, not including the last 60 residues. two ... | 2016 | 26914207 |
| identification of type iii secretion substrates of chlamydia trachomatis using yersinia enterocolitica as a heterologous system. | chlamydia trachomatis is an obligate intracellular human pathogen causing ocular and urogenital infections that are a significant clinical and public health concern. this bacterium uses a type iii secretion (t3s) system to manipulate host cells, through the delivery of effector proteins into their cytosol, membranes, and nucleus. in this work, we aimed to find previously unidentified c. trachomatis t3s substrates. | 2014 | 24533538 |
| f18ab escherichia coli flagella expression is regulated by acyl-homoserine lactone and contributes to bacterial virulence. | to investigate the effect of the quorum sensing (qs)-i system on the expression of virulence factors in shiga toxin producing and verotoxin-producing escherichia coli (stec and vtec), the yeni gene from yersinia enterocolitica was cloned into e. coli f18ab 107/86. recombinant e. coli transformed with yeni produced acyl-homoserine lactone synthase (ahl), as measured using cross-streaking assays with the reporter biosensor strain chromobacterium violaceum cv026. the ai-1 positive recombinant f18ab ... | 2013 | 23693029 |
| inhibition of quorum sensing (qs) in yersinia enterocolitica by an orange extract rich in glycosylated flavanones. | flavanones, flavonoids abundant in citrus , have been shown to interfere with quorum sensing (qs) and affect related physiological processes. we have investigated the qs-inhibitory effects of an orange extract enriched in o-glycosylated flavanones (mainly naringin, neohesperidin, and hesperidin). the qs-inhibitory capacity of this extract and its main flavanone components was first screened using the bacteriological monitoring system chromobacterium violaceum . we next examined the ability of th ... | 2012 | 22533445 |
| characterization of phicfp-1, a virulent bacteriophage specific for citrobacter freundii. | citrobacter freundii, a gram-negative bacterium, causes many opportunistic infections. bacteriophage phicfp-1 was isolated and characterized by its ability to lyse the multidrug-resistant clinical c. freundii strain p10159. transmission electron microscopy showed that the phage has an icosahedral head and a short tail, making it a podoviridae family member. in a single-step growth experiment, phicfp-1 exhibited an eclipse period of 20 min and a burst size of 100 particles per cell. its genome as ... | 2016 | 26455439 |
| a decade of development of chromogenic culture media for clinical microbiology in an era of molecular diagnostics. | in the last 25 years, chromogenic culture media have found widespread application in diagnostic clinical microbiology. in the last decade, the range of media available to clinical laboratories has expanded greatly, allowing specific detection of additional pathogens, including pseudomonas aeruginosa, group b streptococci, clostridium difficile, campylobacter spp., and yersinia enterocolitica. new media have also been developed to screen for pathogens with acquired antimicrobial resistance, inclu ... | 2017 | 28122803 |
| evaluation of the biofire filmarray® gastrointestinalpanel in a midwestern academic hospital. | the biofire filmarray® gastrointestinal panel (gip) was implemented to replace traditional stool culture and enzyme immunoassay (eia) testing for stool pathogens. the purpose of this study was to evaluate the detection rate, incidence of coinfection, and culture recovery rate of gastrointestinal (gi) pathogens detected by the gip over a 1-year period. a total of 2257 stools collected from january to december 2015 were tested using the gip. clostridium difficile colonization was also evaluated by ... | 2017 | 27957599 |
| rapid identification of the etiological factors causing diarrheal diseases. | the main objective of this investigation was to develop and pilot a real-time polymerase chain reaction (rt-pcr) diagnostic system for rapid and simultaneous identification of pathogens with a particular emphasis on diarrheal disease diagnostics. the diarrheal diseases were selected as a target for the pilot because they constitute a primary public health priority in georgia and worldwide. the product developed by our research team "neo_pcr_diagnostics" represents an original system for the iden ... | 2016 | 27770538 |
| [the application and epidemiological research of xtag gpp multiplex pcr in the diagnosis of infectious diarrhea]. | to investigate the application value of xtag (®) gastrointestinal pathogen panel (xtag9(®) gpp) multiplex pcr in the early diagnosis of infectious diarrhea, and understand the epidemiology of intestinal diarrhea pathogens. | 2015 | 25916780 |
| evaluation of a multiplex pcr assay for simultaneous detection of bacterial and viral enteropathogens in stool samples of paediatric patients. | we evaluated a multiplex pcr assay, the seeplex diarrhoea ace detection, that simultaneously detects 15 enteric pathogens, including salmonella spp., shigella spp., vibrio spp., toxin b producer clostridium difficile, campylobacter spp., clostridium perfringens, yersinia enterocolitica, aeromonas spp., escherichia coli o157:h7, verocytotoxin-producing escherichia coli, adenovirus, group a rotavirus, norovirus gi and gii, and astrovirus. we compared this assay with clinical methods routinely used ... | 2014 | 24656922 |
| improved detection of gastrointestinal pathogens using generalised sample processing and amplification panels. | we aimed to streamline the diagnosis of gastrointestinal disease by producing multiplexed real time polymerase chain reaction (pcr) panels employing universal sample processing for dna and rna containing pathogens. a total of 487 stored, previously characterised stool samples comprising bacterial, viral, protozoan and clostridium difficile positive samples were tested using four multiplexed real time pcr panels. a further 81 pre-selected clinical samples from a teaching hospital were included to ... | 2014 | 24300711 |
| added value of multiplex luminex gastrointestinal pathogen panel (xtag® gpp) testing in the diagnosis of infectious gastroenteritis. | the luminex gastrointestinal pathogen panel (xtag(®) gpp) detects in one assay the most common gastroenteritis-causing pathogens and toxins, namely adenovirus 40/41, norovirus genogroup (ng) i/ii, rotavirus a, clostridium difficile toxin a/b, campylobacter sp., escherichia coli o157, enterotoxigenic e. coli heat-labile enterotoxin/heat-stable enterotoxin, salmonella sp., shiga-toxin producing e. coli, shiga-like toxin (stx)1/2, shigella sp., vibrio cholerae, yersinia enterocolitica, cryptosporid ... | 2014 | 24131399 |
| occurrence of zoonotic clostridia and yersinia in healthy cattle. | zoonotic pathogens are a frequent cause of disease worldwide. this study was designed to determine the occurrence of clostridium difficile, clostridium botulinum, and yersinia enterocolitica in cattle in southern bavaria, germany. the study population included 49 farms; 34 were dairy farms (30 also fattening beef cattle) and 15 were solely beef cattle farms. fecal and dust samples were collected from summer 2011 to summer 2012 and analyzed using a combination of enrichment procedures and real-ti ... | 2013 | 24112568 |
| yersinia enterocolitica yopt and clostridium difficile toxin b induce expression of gilz in epithelial cells. | glucocorticoid induced-leucine zipper (gilz) has been shown to be induced in cells by different stimuli such as glucocorticoids, il-10 or deprivation of il-2. gilz has anti-inflammatory properties and may be involved in signalling modulating apoptosis. herein we demonstrate that wildtype yersinia enterocolitica which carry the pyv plasmid upregulated gilz mrna levels and protein expression in epithelial cells. infection of hela cells with different yersinia mutant strains revealed that the prote ... | 2012 | 22792400 |
| [the new microbiological hazards in food]. | this paper describes the new microbiological hazards in food. for protecting human health, nowadays food safety authorities face with many challenges, that years ago were largely unheard. in 2011 verocytotoxigenic escherichia coli o104:h4 has been isolated in germany. strain came from fenugreek sprouts originated from egypt. it was characterized by unique features such as presence of enteroaggregative escherichia coli genes (aata, aggr, aap, agga, aggc) and resistance to most antibiotics. in pol ... | 2012 | 23631259 |
| targeting and alteration of tight junctions by bacteria and their virulence factors such as clostridium perfringens enterotoxin. | the integrity of tight junctions, which regulate paracellular permeability, is challenged by many bacterial pathogens. this is caused by inflammatory responses triggered by pathogens and direct interaction of bacteria or their toxins with host epithelial cells. in some cases, tight junction proteins represent receptors for cell surface proteins or toxins of the pathogen, such as clostridium perfringens enterotoxin (cpe). cpe causes diarrhea and cramps-the symptoms of a common foodborne illness, ... | 2017 | 27864644 |
| disease-outcome trees, eq-5d scores, and estimated annual losses of quality-adjusted life years (qalys) for 14 foodborne pathogens in the united states. | measures of disease burden such as quality-adjusted life years (qalys) are increasingly important to risk-based food safety policy. they provide a means of comparing relative risk from diverse health outcomes. we present detailed disease-outcome trees and eq-5d scoring for 14 major foodborne pathogens representing over 95% of foodborne illnesses, hospitalizations, and deaths due to specified agents in the united states (campylobacter spp., clostridium perfringens, cryptosporidium parvum, cyclosp ... | 2014 | 24588853 |
| green conversion of agroindustrial wastes into chitin and chitosan by rhizopus arrhizus and cunninghamella elegans strains. | this article sets out a method for producing chitin and chitosan by cunninghamella elegans and rhizopus arrhizus strains using a green metabolic conversion of agroindustrial wastes (corn steep liquor and molasses). the physicochemical characteristics of the biopolymers and antimicrobial activity are described. chitin and chitosan were extracted by alkali-acid treatment, and characterized by infrared spectroscopy, viscosity and x-ray diffraction. the effectiveness of chitosan from c. elegans and ... | 2014 | 24853288 |
| [the structure and significance of enterobacterial common antigen (eca)]. | the enterobacterial common antigen (eca) is a carbohydrate-derived cell surface antigen present in all gram-negative bacteria belonging to enterobacteriaceae family. biosynthetic pathways shared by eca and lps (endotoxin) suggest close connections between these antigens. eca occurs in three different forms: a phosphatidyl-linked linear polysaccharide anchored on the cell surface (ecapg), a cyclic form built of 4-6 repeating units localized in the periplasm (ecacyc) and as a linear polysaccharide ... | 2015 | 26400887 |
| bacterial pectate lyases, structural and functional diversity. | pectate lyases are enzymes involved in plant cell wall degradation. they cleave pectin using a β-elimination mechanism, specific for acidic polysaccharides. they are mainly produced by plant pathogens and plant-associated organisms, and only rarely by animals. pectate lyases are also commonly produced in the bacterial world, either by bacteria living in close proximity with plants or by gut bacteria that find plant material in the digestive tract of their hosts. the role of pectate lyases is ess ... | 2014 | 25646533 |
| the landscape of host transcriptional response programs commonly perturbed by bacterial pathogens: towards host-oriented broad-spectrum drug targets. | the emergence of drug-resistant pathogen strains and new infectious agents pose major challenges to public health. a promising approach to combat these problems is to target the host's genes or proteins, especially to discover targets that are effective against multiple pathogens, i.e., host-oriented broad-spectrum (hobs) drug targets. an important first step in the discovery of such drug targets is the identification of host responses that are commonly perturbed by multiple pathogens. | 2013 | 23516507 |
| intestinal microbial community diversity between healthy and orally infected rabbit with entamoeba histolytica by eric-pcr. | enterobacterial repetitive intergenic consensus (eric)-pcr was applied to analyze the difference of intestinal microbial community diversity between healthy and orally infected rabbits with entamoeba histolytica. the dynamic changes in different parts of digestive system including the duodenum, jejunum, ileum, caecum, and rectum in healthy and infected rabbits at different time points were also tested. the intestinal microbial community of the control healthy rabbits was steady, and the total nu ... | 2012 | 22562215 |
| development of pla films containing oregano essential oil (origanum vulgare l. virens) intended for use in food packaging. | consumers' concerns about the environment and health have led to the development of new food packaging materials avoiding petroleum-based matrices and synthetic additives. the present study has developed polylactic acid (pla) films containing different concentrations of essential oil from origanum vulgare l. virens (oeo). the effectiveness of this new active packaging was checked for use in ready-to-eat salads. a plasticising effect was observed when oeo was incorporated in pla films. the rest o ... | 2016 | 27455176 |
| experimental studies of microbial populations and incidence of zoonotic pathogens in the faeces of red deer (cervus elaphus). | wild animals can serve as hosts, amplifiers or reservoirs for various zoonotic diseases. most species of deer in highly fragmented agricultural landscapes, search out maximum cover from intrusive human activity. hence, the likelihood of zoonosis transmission is likely to increase the more humans and wildlife interact. in our study, we conducted a comparative analysis of bacteria isolated from the faeces of red deer (cervus elaphus) living in their natural environment in south-western poland and ... | 2015 | 26222832 |
| usefulness of in-house obtained recombinant proteins yop of yersinia enterocolitica as highly specific antigens in elisa and recom-dot performed in the serodiagnosis of yersiniosis. | proper analysis of the human immune response is crucial in the laboratory diagnosis of many bacterial infections-the current serological diagnosis of yersiniosis often is carried out using elisa with native antigens. however, recombinant proteins increase the specificity of the serological assays, particularly in patients with chronic, non- specific infections. the aim of the present study was to evaluate the usefulness of in-house obtained recombinant proteins yop of yersinia enterocolitica as ... | 2017 | 28146619 |
| bacterium-derived cell-penetrating peptides deliver gentamicin to kill intracellular pathogens. | commonly used antimicrobials show poor cellular uptake and often have limited access to intracellular targets, resulting in low antimicrobial activity against intracellular pathogens. an efficient delivery system to transport these drugs to the intracellular site of action is needed. cell-penetrating peptides (cpps) mediate the internalization of biologically active molecules into the cytoplasm. here, we characterized two cpps, α1h and α2h, derived from the yersinia enterocolitica yopm effector ... | 2017 | 28096156 |
| prevalence, seasonal variation, and antibiotic resistance pattern of enteric bacterial pathogens among hospitalized diarrheic children in suburban regions of central kenya. | the epidemiology of enteric pathogens has not been well studied in kenya because of wide disparities in health status across the country. therefore, the present study describes the prevalence of enteropathogenic bacteria, their seasonal variation, and antibiotic resistance profiles among hospitalized diarrheic children in a suburban region of central kenya. | 2016 | 27942243 |
| the phage shock protein response. | the phage shock protein (psp) system was identified as a response to phage infection in escherichia coli, but rather than being a specific response to a phage, it detects and mitigates various problems that could increase inner-membrane (im) permeability. interest in the psp system has increased significantly in recent years due to appreciation that psp-like proteins are found in all three domains of life and because the bacterial psp response has been linked to virulence and other important phe ... | 2016 | 27297125 |