| vectors and agrobacterium hosts for arabidopsis transformation. | introductionarabidopsis can be stably transformed using agrobacterium tumefaciens-mediated transfer of t-dna. a. tumefaciens is a soil-dwelling bacterium that transforms normal plant cells into tumor-forming cells by inserting a piece of bacterial dna (the transfer, or "t," dna) into the plant cell genome. the t-dna, which is flanked by left- and right-border (lb and rb) sequences, resides on a tumor-inducing (ti) plasmid. the ti plasmid also carries many of the transfer functions for mobilizing ... | 2006 | 22484668 |
| overexpression and protein folding of a chimeric beta-glucosidase constructed from agrobacterium tumefaciens and cellvibrio gilvus. | in continuation of our investigation on structure and function relationship of beta-glucosidases from mesophilic and thermophilic bacteria, we constructed a chimeric gene by shuffling 17% length in c terminal region of beta-glucosidase of agrobacterium tumefaciens with the corresponding homologous region of cellvibrio gilvus beta-glucosidase. the chimeric gene was overexpressed in e. coli bl21 (de3) using pet vector. however, nearly all of the beta-glucosidase produced was trapped into inclusion ... | 2002 | 22908413 |
| transformation of the limonene synthase gene into peppermint (mentha piperita l.) and preliminary studies on the essential oil profiles of single transgenic plants. | agrobacterium-mediated and direct gene transfer into protoplasts using peg were both successfully used to produce stable, transformed peppermint plants (mentha×piperita l. cultivar black mitcham) with the limonene synthase gene. stem internode explants found to possess a high level of organogenesis through adventitious shoot formation were subjected to agrobacterium tumefaciens disarmed strain gv3101 (pmp90). following the development of an efficient protoplast-to-plant cycle from stem-isolated ... | 1999 | 22665205 |
| a rapid method for transformation of carrot (daucus carota l.) by using direct somatic embryogenesis. | several genes that may be involved in embryogenesis have been isolated from somatic embryos of carrot by many workers. however, the function of these genes has not been discovered yet. as the first step toward finding the function of these genes, we established a rapid and efficient method for transformation of carrot by using direct embryogenesis from hypocotyl segments treated with 2,4-dichlorophenoxyacetic acid (2,4-d) for a short period. | 1999 | 27393260 |
| inhibition of plant transformation by phytolaccoside b from phytolacca americana callus. | the newly established gus expression bioassay on the callus extracts of 22 species of plants revealed that the methanol extract of phytolacca americana callus had the most potent inhibitory activity against agrobacterial plant transformation. a triterpene glycoside phytolaccoside b was isolated from the extract as a genuine plant transformation inhibitor having neither antiagrobacterial nor phytotoxic activity. this compound is promising for use as a biochemical probe for studies on the plant tr ... | 1999 | 27389651 |
| improved bioassay method for plant transformation inhibitors. | a convenient and quantitative bioassay method for evaluating the efficiency of plant transformation by agrobacterium tumefaciens is important to search plant transformation inhibitors, possible biochemical probes for study on its mechanism. our previously reported method, in which the plant transformation had been detected by the expression of β-glucuronidase in transformed plants, was improved. the difference between the previous and the improved methods is the use of suspension-cultured cells ... | 1998 | 27392395 |
| genetic transformation of cotyledon explants of cowpea (vigna unguiculata l. walp) using agrobacterium tumefaciens. | mature de-embryonated cotyledons with intact proximal end of vigna unguiculata were cultured on b5 basal medium containing varying concentrations of bap. thirty-six percent of the explants produced shoots on b5 medium supplemented with 8× 10(-6) m bap. cotyledon explants were pre-incubated for 24 h, inoculated with a. tumefaciens pucd2614 carrying pucd2340, co-cultivated for 48 h and transferred to hygromycin-b (25 mg/l) containing shoot induction medium. approximately 15-19% of the explants pro ... | 1996 | 24178287 |
| agrobacterium tumefaciens-mediated transformation of artemisia annua l. and regeneration of transgenic plants. | a transformation system was developed for artemisia annua l. plants. leaf explants from in vitro grown plants developed callus and shoots on medium with 0.05 mg/l naphthaleneacetic acid and 0.5 mg/l n(6)-benzyladenine after transformation with the c58c1 rif(r) (pgv2260) (ptjk136) agrobacterium tumefaciens strain. a concentration of 20 mg/l kanamycin was added in order to select transformed tissue. kanamycin resistant shoots were rooted on naphthaleneacetic acid 0.1 mg/l. polymerase chain reactio ... | 1996 | 24178277 |
| transformation of cucumber (cucumis sativus l.) plants using agrobacterium tumefaciens and regeneration from hypocotyl explants. | transgenic cucumber (cucumis sativus l.) plants were successfully obtained from hypocotyl explants inoculated with agrobacterium tumefaciens, which harbored a binary vector plasmid with nos-nptii, camv 35s-i-gus and camv 35s-hph genes. acetosyringone enhanced the efficiency of transformation at the cut surface cells of hypocotyl explants during five days of co-cultivation. transformed cells were more effectively selected using 20-30 mg/l hygromycin b than using 50-100 mg/l kanamycin. shoot regen ... | 1996 | 24178213 |
| transgenic plant production from leaf discs of moricandia arvensis using agrobacterium tumefaciens. | a high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of moricandia arvensis. leaf discs were shown to be a preferable starting material for transformation experiments. agrobacterium tumefaciens strain gv3101/pmp90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (gus). maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a sel ... | 1996 | 24178211 |
| transgenic plant production mediated by agrobacterium in indica rice. | a reproducible system has been developed for the production of transgenic plants in indica rice using agrobacterium-mediated gene transfer. three-week-old scutella calli served as an excellent starting material. these were infected with an agrobacterium tumefaciens strain eha101 carrying a plasmid pig121hm containing genes for β-glucuronidase (gus) and hygromycin resistnace (hygr). hygromycin (50 mg/l) was used as a selectable agent. inclusion of acetosyringone (50μm) in the agrobacterium suspen ... | 1996 | 24178159 |
| forskolin synthesis in in vitro cultures of coleus forskohlii briq transformed with agrobacterium tumefaciens. | agrobacterium tumefaciens mediated tumor tissue and shooty teratomas of coleus forskohlii were cultured in vitro. forskolin was detected in tumorous callus (0.002%), rhizogenic callus (0.011%) and root cultures (0.014%), but not in shooty teratomas. forskolin synthesis and accumulation in tumorous c. forskohlii cultures may permit the elucidation of diterpene metabolism in this species. | 1996 | 24178612 |
| production of fertile transgenic peanut (arachis hypogaea l.) plants using agrobacterium tumefaciens. | fertile transgenic plants of peanut (arachis hypogaea l. cv. new mexico valencia a) were produced using an agrobacterium-mediated transformation system. leaf section explants were inoculated with a. tumefaciens strain eha105 harboring the binary vector pbi121 containing the genes for β-glucuronidase (gus) and neomycin phosphotransferase ii (nptii). approximately 10% of the shoots regenerated on selection medium were gus-positive. five independent transformation events resulted in the production ... | 1996 | 24178604 |
| shoot regeneration and agrobacterium-mediated transformation of fragaria vesca l. | an efficient and reliable method for shoot regeneration from leaf disks of fragaria vesca l. has been developed. this protocol has been successfully employed to obtain transformed plants using agrobacterium tumefaciens as gene vector. murashige and skoog basal medium supplemented with benzyladenine (4 mg/l) and indole-3-butyric acid (0.25 mg/l) induced the maximum percentage of shoot regeneration (98%) and the highest number of shoot colonies per explant (4.6) after 8 weeks of culture. isolated ... | 1996 | 24178534 |
| genetic transformation of begonia tuberhybrida by ri rol genes. | we have developed an agrobacterium -mediated transformation system for commercial begonia species. the leaf explants of begonia semperflorens, begonia x hiemalis and b. tuberhybrida were inoculated with agrobacterium tumefaciens lba4404 harboring a binary vector pbi121 which contains rola, b and c genes of an agropine type ri plasmid (pria4b). kanamycin resistant shoots of b. tuberhybrida were obtained on ms agar medium supplemented with 0.1 mg/l naa, 0.5 mg/l ba, 500 mg/l claforan and 100 mg/l ... | 1996 | 24178527 |
| transformation of pickling cucumber with chitinase-encoding genes using agrobacterium tumefaciens. | transformation of cucumber cv. endeavor was attempted using three agrobacterium tumefaciens strains (a supervirulent leucinopine type, an octopine type and a nopaline type), each harbouring one of three binary vectors which contained an acidic chitinase gene from petunia, and basic chitinase genes from tobacco and bean, respectively, driven by the camv 35s promoter. petiole explants were inoculated with a bacterial suspension (10(8) cells·ml(-1)), cocultivated for 48-96 h and placed on murashige ... | 1996 | 24178524 |
| agrobacterium tumefaciens-mediated transformation of solanum gilo raddi as influenced by explant type. | an efficient system for agrobacterium tumefaciens-mediated transformation of solanum gilo was established. the marker genes for kanamycin resistance and ß-glucuronidase expression were introduced. a comparison between cotyledon and hypocotyl explants showed that while regeneration was better from hypocotyl explants, cotyledon explants gave better transformation efficiency (46% vs. 32%). four levels of kanamycin selection (100, 150, 200 and 250 mg/l) were tested for effect on transformation effic ... | 1996 | 24178522 |
| improvements in the transformation of arabidopsis thaliana c24 leaf-discs by agrobacterium tumefaciens. | we report here an efficient arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces seeds with an average seed yield of 100-300 seeds per plant. this improved transformation protocol yields mainly (70%) transformants segregating for a single t-dna locus of which 68% a ... | 1996 | 24178520 |
| agrobacterium-mediated transformation of apple (malus x domestica borkh.): an assessment of factors affecting regeneration of transgenic plants. | we have previously developed a protocol for efficient gene transfer and regeneration of transgenic calli following cocultivation of apple (cv. jonagold) explants with agrobacterium tumefaciens (de bondt et al. 1994, plant cell reports 13: 587-593). now we report on the optimization of postcultivation conditions for efficient and reproducible regeneration of transgenic shoots from the apple cultivar jonagold. factors which were found to be essential for efficient shoot regeneration were the use o ... | 1996 | 24178471 |
| agrobacterium t-dna mutation causes the loss of gus expression in transgenic tobacco. | a new freezer stock of an agrobacterium tumefaciens clone of lba4404/pbi121, designated 8999, was found to contain a mutation in the t-dna region. gus activity in agrobacterium 8999 was reduced to levels in negative controls of lba4404. additionally, gus activity in t1 seedlings from tobacco plants transformed with 8999 was reduced to that of untransformed plants. southern and northern blotting showed that agrobacterium clone 8999 transferred its t-dna into the plant, that the correct sizes of 3 ... | 1996 | 24178420 |
| agrobacterium tumefaciens-mediated transformation of vigna mungo (l.) hepper. | transformed vigna mungo (blackgram) calli were obtained by cocultivating segments of primary leaves with agrobacterium tumefaciens vir helper strains harbouring the binary vector pga472 having kanamycin resistance gene as plant transformation marker. transformed calli were selected on murashige and skoog medium supplemented with 50 mg/l kanamycin and 500 mg/l carbenicillin. transformed calli were found to be resistant to kanamycin up to 900 mg/l concentration. expression of kanamycin resistance ... | 1996 | 24178351 |
| transformation of barrel medic (medicago truncatula gaertn.) by agrobacterium tumefaciens and regeneration via somatic embryogenesis of transgenic plants with the mtenod12 nodulin promoter fused to the gus reporter gene. | fertile and stable transgenic plants of the model legume medicago truncatula gaertn. were obtained through transformation of leaf tissue with the disarmed agrobacterium tumefaciens strain lba4404 and in vitro regeneration via somatic embryogenesis. an optimised transformation/regeneration protocol has been established for two genotypes of the cultivar jemalong, including a previously described highly embryogenic line (nolan et al. 1989, plant cell rep. 8: 278-281). using this protocol, transgeni ... | 1996 | 24178347 |
| agrobacterium tumefaciens-mediated transformation of broccoli (brassica oleracea var. italica) and cabbage (b. oleracea var. capitata). | transgenic broccoli (brassica oleracea var. italica) was produced by two agrobacterium tumefaciens-mediated transformation methods. one used flowering stalk explants from mature plants; the other used hypocotyl and petiole explants from in vitro-grown seedlings. several hundred transformants containing a bacillus thuringiensis ∂-endotoxin gene (cryia(c)-type) and the neomycin phosphotransferase gene were recovered. rooted transformants were obtained in as little as 3 months using seedling explan ... | 1995 | 24185794 |
| transformation of peas (pisum sativum l.) using immature cotyledons. | a reliable agrobacterium tumefaciens-mediated transformation method has been developed for peas (pisum sativum) using immature cotyledons as the explant source. transgenic plants were recovered from the four cultivars tested: bolero, trounce, bohatyr and huka. the method takes approximately 7 months from explant to seed-bearing primary regenerant. the binary vector used carried genes for kanamycin and phosphinothricin resistance. transformed pea plants were selected on 10 mg/l phosphinothricin. ... | 1995 | 24185786 |
| transformation of solanum brevidens using agrobacterium tumefaciens. | leaf pieces of in vitro-cultured plantlets of the wild potato species solanum brevidens phil. were cocultivated with agrobacterium tumefaciens that contained nptii and uida genes on the disarmed plasmid pbi121. independent transgenic shoots were regenerated from solidified and liquid medium that contained 50 mg l(-1) kanamycin. two agrobacterium strains were investigated for transformation efficiency. gv2260, which contained p35sgusint, resulted in a 11% transformation frequency, compared with 1 ... | 1995 | 24185775 |
| agrobacterium-mediated transformation of peanut (arachis hypogaea l.) embryo axes and the development of transgenic plants. | transgenic peanut (arachis hypogaea l.) plants have been produced using an agrobacterium-mediated transformation system. zygotic embryo axes from mature seed were cocultured with agrobacterium tumefaciens strain eha101 harboring a binary vector that contained the genes for the scorable marker b-glucuronidase (gus) and the selectable marker neomycin phosphotransferase ii. nine percent of the germinated seedlings were gus+. polymerase chain reaction analysis confirmed that gus+ shoots and t1 proge ... | 1995 | 24186625 |
| asymmetric somatic hybrid plants between an interspecific lycopersicon hybrid and solanum melongena. | asymmetric somatic hybrid plants were obtained by a modified peg/dmso fusion procedure between protoplasts derived from suspension cells of an interspecific tomato hybrid, lycopersicon esculentum x l. pennellii, and mesophyll protoplasts of solanum melongena, eggplant. the tomato hybrid was previously transformed with agrobacterium tumefaciens and contained the kanamycin-resistance marker gene. prior to fusion, the donor protoplasts of the tomato hybrid were gamma irradiated at 9.0 krad. thus, n ... | 1995 | 24194315 |
| agrobacterium-mediated transformation and regeneration of fertile transgenic plants of chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor'). | a procedure for the regeneration of fertile transgenic chinese cabbage (brassica campestris ssp. pekinensis cv. 'spring flavor') is presented in this report. the protocol is based on infection of cotyledon explants of 5-d-old seedlings with an agrobacterium tumefaciens strain lba4404 carrying a disarmed binary vector ptok/bks-1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower m ... | 1995 | 24194308 |
| agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants. | transgenic sweet orange (citrus sinensis l. osbeck) plants have been obtained by agrobacterium tumefaciens-mediated gene transfer. an hypervirulent a. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase ii (npt ii) and ß-glucuronidase (gus) genes was cocultivated with stem segments from in vivo grown seedlings. shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. shoot basal portions were assayed for g ... | 1995 | 24194307 |
| transformation of grape (vitis vinifera l.) zygotic-derived somatic embryos and regeneration of transgenic plants. | transgenic grape plants were regenerated from somatic embryos derived from immature zygotic embryos of seedless grape (vitis vinifera l.) selections. somatic embryos were bombarded twice with 1 μm gold particles using the biolistic pds-1000/he device (bio-rad laboratories) and then exposed to agrobacterium tumefaciens strain c58/z707 containing the binary plasmid pga482gg or pcgn7314. following cocultivation, secondary embryos were allowed to proliferate on emershad/ramming proliferation (erp) m ... | 1995 | 24185603 |
| transformation of grapevine rootstocks with the coat protein gene of grapevine fanleaf nepovirus. | control of fanleaf disease induced by the grapevine fanleaf nepovirus (gflv) today is based on sanitary selection and soil disinfection with nematicides. this way of control is not always efficient and nematicides can be dangerous pollutants. coat protein (cp) mediated protection could be an attractive alternative. we have transferred a chimeric cp gene of gflv-f13 via agrobacterium tumefaciens lba4404 into two rootstock varieties: vitis rupestris and 110 richter (v. rupestris x v. berlandieri). ... | 1995 | 24185595 |
| efficient genetic transformation of red raspberry, rubus ideaus l. | we have developed an efficient transformation system for red raspberry (rubus ideaus l.) using agrobacterium mediated gene transfer. using this system we have successfully introduced a gene that encodes an enzyme, s-adenosylmethionine hydrolase (samase), in raspberry cultivars meeker (mk), chilliwack (ch) and canby (cy). leaf and petiole expiants were inoculated with disarmed agrobacterium tumefaciens strain eha 105 carrying either of two binary vectors, pag1452 or pag1552, encoding gene sequenc ... | 1995 | 24185514 |
| regeneration of transgenic plants from the commercial apple cultivar royal gala. | a transformation system was developed for the commercial apple (malus x domestica borkh.) cultivar royal gala. leaf pieces from in vitro-grown shoots were cocultivated for 2 days with agrobacterium tumefaciens strain lba4404 containing the binary vectors pkiwi105 or pkiwi110. shoots were produced on a shooting medium containing kanamycin (50 mg·l(-1)). a 2-day incubation period on kanamycin-free medium prior to antibiotic selection enhanced the regeneration of kanamycin-resistant shoots. the maj ... | 1995 | 24185446 |
| transformation of sunflower (helianthus annuus l.) following wounding with glass beads. | a procedure was developed for transformation of helianthus annuus (sunflower) using agrobacterium tumefaciens. cotyledons were removed from young seedlings, and the remaining tissue was uniformly wounded by shaking with glass beads. the wounded tissue was then co-cultivated with a hypervirulent strain of agrobacterium tumefaciens harboring the binary plasmid pcnl56. minimal use of defined medium was required, and no callus was observed. the polymerase chain reaction (pcr) followed by dna hybridi ... | 1995 | 24186761 |
| agrobacterium-mediated transformation of almond leaf pieces. | a protocol for agrobacterium-mediated transformation of leaf pieces from two almond cultivars has been developed. this protocol gave a transformation percentage of 48.6 for the cultivar mn51 and 27.9 for the cultivar 'supernova'. the agrobacterium tumefaciens strain lba 4404 carrying a binary vector plasmid pbingusint has been used. a critical step for optimal transformation was the pre-culture period of leaf pieces prior selection on medium containing kanamycin. both cultivars showed an almost ... | 1995 | 24186758 |
| genetic transformation ofpelargonium x hortorum. | transgenicpelargonium x hortorum have been producedvia agrobacterium tumefaciens-mediated transformation. the regeneration protocol used provided a regeneration frequency approximately to 95 percent. clumps of regenerants, from cotyledons and hypocotyls ofpelargonium x hortorum seedlings, were inoculated with the disarmed strain eha101 ofagrobacterium tumefaciens. this strain contains a binary vector carrying neomycin phosphotransferase ii, hygromycin b phosphotransferase and ß-glucuronidase gen ... | 1995 | 24185656 |
| production of virus resistant and insect tolerant transgenic tobacco plants. | the cucumber mosaic virus coat protein (cmv-cp) gene and a modified bacillus thuringiensis δ-endotoxin (bt toxin) gene were cloned into plant expression vector pe3. tobacco (nicotiana tabacum cv. g28) leaf discs were transformed with agrobacterium tumefaciens a12 carrying recombinant pe14. transgenic r0 and r1 tobacco plants expressing cmv-cp and bt toxin genes were protected from cmv infection as well as feeding damage of manduca sexta (tobacco hornworm) larvae. these results demonstrate that i ... | 1994 | 24192882 |
| agrobacterium-mediated transformation of the desiccation-tolerant plant craterostigma plantagineum. | an efficient procedure for agrobacterium tumefaciens- mediated transformation of the desiccation-tolerant plant craterostigma plantagineum has been developed. leaf explants were inoculated with a. tumefaciens strain gv3101 carrying the gene for kanamycin- or hygromycin-resistance and the ßglucuronidase reporter gene. parameters which affected the transformation efficiency were the age of the explant, the degree of wounding and the presence of an antioxidant in the medium. under optimal condition ... | 1994 | 24192874 |
| agrobacterium-mediated transformation of quaking aspen (populus tremuloides) and regeneration of transgenic plants. | agrobacterium-mediated gene transformation of populus tremuloides michx was accomplished by co-cultivation of leaf disks excised from greenhouse plants with agrobacterium tumefaciens containing a binary ti-plasmid vector harboring chimeric neomycin phosphotransferase (npt ii) and ß-glucuronidase (gus) genes. shoot regeneration in the presence of kanamycin was achieved when thidiazuron (tdz) was used as a plant growth regulator. transformation was verified by amplification of npt ii and gus gene ... | 1994 | 24192872 |
| transformation of sunflower (helianthus annuus l.): a reliable protocol. | a reliable protocol for the transformation of cultivated sunflower (helianthus annuus l.) has been established, based on microprojectile bombardment of half shoot apices in combination with agrobacterium tumefaciens coculture. transgenic shoots have been obtained from 5 inbred lines, although transformation efficiencies varied with the genotype. plants expressing the transgenes could be recovered from up to 7% of the explants. a minority of plants was shown to be chimaeric for expression of ß-gl ... | 1994 | 24192870 |
| stable expression of the gus reporter gene in chrysanthemum depends on binary plasmid t-dna. | three chrysanthemum (dendranthema grandiflora) cultivars were cocultivated with 2 agrobacterium tumefaciens strains in combination with 4 pbin19 derived binary plasmids, all carrying the nosnptll selection gene and 35sgus(intron) reporter gene. all binary plasmids transferred dna to chrysanthemum explants but only pmog410 gave good stable expression of gus. this plasmid differs from the other plasmids in 2 aspects: 1) it carries a restored nptll gene and 2) the selection gene is positioned at th ... | 1994 | 24194229 |
| introduction and expression of an insect proteinase inhibitor in alfalfa medicago sativa l. | as one approach to alleviating the need for insecticide spraying, our objective is to express protein insecticides in transgenic alfalfa. to initiate these studies, a cdna encoding the protease inhibitor (pi) anti-elastase from manduca sexta was placed under the control of the camv 35s promoter, inserted into pan 70, and transferred into leaf and petiole sections of alfalfa (medicago sativa l.) using agrobacterium tumefaciens mediated gene transfer. transformation rates were 10% of all explants ... | 1994 | 24194223 |
| transgenic plums (prunus domestica l.) express the plum pox virus coat protein gene. | plum hypocotyl slices were transformed with the coat protein (cp) gene of plum pox virus (ppv-cp) following cocultivation with agrobacterium tumefaciens containing the plasmid pga482gg/ppvcp-33. this binary vector carries the ppv-cp gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidase genes. integration and expression of the transferred genes into regenerated plum plants was verified through kan resistance, gus assays, and pcr amplification of the ppv-cp gene. ... | 1994 | 24194220 |
| transformation of passionfruit (passiflora edulis fv flavicarpa degener.) using agrobacterium tumefaciens. | leaf and stem explants of passionfruit (passiflora eadulis fv flavicarpa) were co-cultivated with a disarmed strain of agrobacterium tunefaciens harbouring the co-integrate vector pmon200. four plants of passionfruit were regenerated from leaf explants on agar-solidified murashige and skoog (1962) based medium containing 4.43 μm 6-benzyl-aminopurine and supplemented with 86 μm kanamycin sulphate. the four plants were rooted by transfer to ms based medium with 14.7 μm 3-indolebutyric acid and 2.6 ... | 1994 | 24193523 |
| transformation of potato (solanum tuberosum) cv. mantiqueira using agrobacterium tumefaciens and evaluation of herbicide resistance. | in search of establishing a system for genetic transformation of brazilian potato cultivars, agrobacterium tumefaciens carrying the plasmid pgv1040, was used to transform leaf discs of three cultivars of local importance, i.e., aracy, baronesa and mantiqueira. this plasmid contains marker genes for resistance to kanamycin and phosphinothricin plus the gene for the enzyme β-glucuronidase. a two step regeneration/selection procedure produced shoots of potato cultivar mantiqueira with in vitro resi ... | 1994 | 24193516 |
| a simple procedure for the expression of genes in transgenic soybean callus tissue. | cotyledons from germinating seeds of the soybean cultivar peking were inoculated with virulent agrobacterium tumefaciens strain a281:pza-7 which carries a wild type ti plasmid ptibo542 and a disarmed ti plasmid (a binary vector)pza-7 which contains the βglucuronidase (uida) and neomycin phosphotransferase (nptii) genes. tumors were produced on all inoculated explants and 82% of these tumor lines were cotransformed by the nptii gene from the binary vector pza-7 as shown by pcr analysis (18 of 22 ... | 1994 | 24196243 |
| agrobacterium-mediated transformation of apple (malus x domestica borkh.): an assessment of factors affecting gene transfer efficiency during early transformation steps. | the factors influencing transfer of an intron - containing β-glucuronidase gene to apple leaf explants were studied during early steps of an agrobacterium tumefaciens-mediated transformation procedure. the gene transfer process was evaluated by counting the number of β-glucuronidase expressing leaf zones immediately after cocultivation, as well as by counting the number of β-glucuronidase expressing calli developing on the explants after 6 weeks of postcultivation in the presence of 50 mg/l kana ... | 1994 | 24196227 |
| agrobacterium tumefaciens mediated gene transfer in peanut (arachis hypogaea l.). | transgenic peanut plants were produced using agrobacterium mediated gene transfer. primary leaf explants of peanut were co-cultivated with agrobacterium tumefaciens lba 4404 harbouring the binary plasmid pbi 121 (conferring β-glucuronidase activity and resistance to kanamycin) and cultured on regeneration medium supplemented with kanamycin to select putatively transformed shoots. they were rooted and plants were transferred to soil. stable integration and expression of the transgenes were confir ... | 1994 | 24196226 |
| strain and cultivar specificity in the agrobacterium-chickpea interaction. | the susceptibility of four genotypes of chickpea to four wild strains of agrobacterium tumefaciens was evaluated. successful transformation was dependent on specific bacterial strain-plant cultivar interactions. agropine strain a281 was the most effective for tumor induction. tumors displayed hormone autonomous growth, were opine positive and contained dna that was homologous to the t-dna of the inciting strain. | 1994 | 24196221 |
| a thin layer chromatographic technique for detecting inducers of agrobacterium virulence genes in corn, wheat and rye. | a method is described for detecting plant metabolites capable of inducing the virulence genes of agrobacterium tumefaciens. the method uses a. tumefaciens containing a plasmid with an inducible virulence gene fused to a galactosidase gene (vire::lacz). thin layer chromatography plates are overlayed with agar containing the indicator bacterium and a chromogenic galactoside (x-gal). virulence gene inducing plant metabolites induce galactosidase which releases an aglycone readily oxidized by air to ... | 1994 | 24194126 |
| expression of a wild-type gbss gene introduced into an amylose-free potato mutant by agrobacterium tumefaciens and the inheritance of the inserts at the microsporic level. | granule-bound starch synthase (gbss) catalyses the synthesis of amylose in starch granules. transformation of a diploid amylose-free (amf) potato mutant with the gene encoding gbss leads to the restoration of amylose synthesis. transformants were obtained which had wild-type levels of both gbss activity and amylose content. it proved to be difficult to increase the amylose content above that of the wild-type potato by the introduction of additional copies of the wild-type gbss gene. staining of ... | 1994 | 24186021 |
| regeneration of plants from leaf explants of micropropagated clonal eucalyptus grandis. | callus production along with caulogenesis was obtained from leaf explants of micropropagated clonal eucalyptus grandis after six to twelve weeks of culture. out of eight clones tested, six were amenable to shoot production using simple media containing naphthaleneacetic acid and either 6-benzyladenine or zeatin. differences in growth regulator requirements for organogenesis were observed between different clones. these shoots were then elongated on a medium containing gibberellic acid and rooted ... | 1994 | 24194029 |
| modification of competence for in vitro response to fusarium oxysporum in tomato cells. ii. effect of the integration of agrobacterium tumefaciens genes for auxin and cytokinin synthesis. | we have studied the effect of a change in the endogenous hormone equilibria on the competence of tomato (lycopersicon esculentum) cells to defend themselves against the fungal pathogen fusarium oxysporum f. sp. lycopersici. calluses from cvs 'davis' and 'red river', respectively resistant and susceptible to fusarium and transgenic for an auxin- or cytokinin-synthesizing gene from agrobacterium tumefaciens, were used. the integration of agrobacterium hormone-related genes into susceptible cv 'red ... | 1994 | 24185887 |
| protection against virus infection in tobacco plants expressing the coat protein of grapevine fanleaf nepovirus. | grapevine fanleaf nepovirus (gflv) is responsible for the economically significant "court-noué" disease in vineyards. its genome is made up of two single-stranded rna molecules (rna1 and rna2) which direct the synthesis of polyproteins p1 and p2 respectively. a chimeric coat protein gene derived from the c-terminal part of p2 was constructed and subsequently introduced into a binary transformation vector. transgenic nicotiana benthamiana plants expressing the coat protein under the control of th ... | 1994 | 24193837 |
| genetic transformation and plant regeneration of watermelon using agrobacterium tumefaciens. | adventitious shoots formed on the proximal cut edges of different cotyledonary explants of watermelon [citrullus lanatus (thunb.) matsum. & nakai; cvs. sweet gem and gold medal] cultured on murashige and skoog's (ms) medium with 1 mgl(-1) 6-benzyladenine (ba). light (16-h photoperiod, about 7 wm(-2) cool-white fluorescent lamps) was essential for shoot formation. to obtain transformed plants, cotyledonary explants of 'sweet gem' were cocultured with agrobacterium tumefaciens lba4404, a disarmed ... | 1994 | 24193834 |
| effect of plant genotype on the transformation of cultivated alfalfa (medicago sativa) by agrobacterium tumefaciens. | the trait for somatic embryogenesis is being introduced sexually into alfalfa (medicago sativa) breeding populations to facilitate genetic transformation of this crop. cocultivation experiments were conducted with an agronomically-improved embryogenic clone from one such population as well as with two other embryogenic clones, one of which was the source of the embryogenic trait in the breeding populations. transgenic plants were produced from the agronomically-improved clone whereas none were p ... | 1994 | 24193831 |
| high efficiency agrobacterium-mediated gene transfer to flax. | using an agrobacterium tumefaciens binary vector (pal4404, pbi131), we have demonstrated the transfer of the β-glucuronidase gene into the flax (linum usitatissimum l.) cultivar glenelg after selection for kanamycin resistance. the transformed lines were obtained by inoculation and subsequent regeneration of hypocotyl segments. the callus that formed on the cut surfaces of the hypocotyl segments was isolated three weeks after infection and was subsequently subcultured to yield shoots. this proce ... | 1994 | 24193765 |
| genetic transformation of populus nigra by agrobacterium tumefaciens. | two clones of populus nigra l. were tested in vivo and in vitro for their susceptibility to three different agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. strain c58 proved to be the most virulent.various parameters affecting agrobacterium-mediated transformation of p. nigra clones were further analyzed using ß-glucuronidase gene transient expression. the clone jean pourtet proved to be more susceptible than the clone san giorgio. a. tumefaciens str ... | 1994 | 24193760 |
| construction of a designer chromosome in tobacco. | the tobacco (nicotiana tabacum l.) breeding line nc 152 is a doubled haploid that possesses an addition chromosome from n. africana [merxm. and buttler]. a gene on this chromosome confers potyvirus resistance (poty(r)). our objective was to use the addition chromosome as a base on which to construct a designer chromosome containing a foreign gene linkage package. a mutant dhfr gene conferring resistance to methotrexate (mtx) was inserted into nc 152-haploid (n = 25) leaf tissue via agrobacterium ... | 1994 | 24190470 |
| the reversible inhibition of pollen germination of nicotiana tabacum l.: an entry into a transformation system. | the hydrodynamics of mature pollen rehydration in nicotiana tabacum was used to study reversible inhibition of pollen germination in vitro. tobacco pollen was incubated for various times in media containing calcium, potassium and magnesium salts, boric acid, and exhibiting different osmotic pressures as a function of sucrose concentration. total inhibition of germination with complete viability preservation was achieved for 56 h by keeping the grains in medium with 80% sucrose, since typical per ... | 1994 | 24193658 |
| biolistic transformation of tobacco and maize suspension cells using bacterial cells as microprojectiles. | we have used both escherichia coli cells and agrobacterium tumefaciens cells as microprojectiles to deliver dna into suspension-cultured tobacco (nicotiana tabacum l. line nt1) cells using a helium powered biolistic device. in addition, e. coli cells were used as microprojectiles for the transformation of suspension-cultured maize (zea mays cv. black mexican sweet) cells. pretreating the bacterial cells with phenol at a concentration of 1.0%, and combining the bacterial cells with tungsten parti ... | 1994 | 24193654 |
| agrobacterium-mediated transformation of commercial melon (cucumis melo l., cv. amarillo oro). | cotyledon explants of muskmelon (cucumis melo l., cv. amarillo oro) seedlings were co-cultivated with disarmed agrobacterium tumefaciens strain lba4404 that contained the binary vector plasmid pbi121.1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower mosaic virus 35s/β-glucuronidase (gus) chimeric gene. after infection, the cotyledon pieces were placed in induction medium conta ... | 1994 | 24193640 |
| agrobacterium-mediated transformation of white mustard (sinapis alba l.) and regeneration of transgenic plants. | a procedure for the regeneration of fertile transgenic white mustard (sinapis alba l.) is presented. the protocol is based on infection of stem explants of 7-9 day old plants with an agrobacterium tumefaciens strain harboring a disarmed binary vector with chimeric genes encoding neomycin phosphotransferase and β-glucuronidase. shoots are regenerated from callus-forming explants within 3-4 weeks. under selection, 10% of the explants with transgenic embryonic callus develop into fertile transgenic ... | 1994 | 24193637 |
| regulatory sequences involved in the peroxidase gene expression in arabidopsis thaliana. | organ-specific expression of two peroxidase genes (prxca and prxea) from arabidopsis thaliana was studied. the prxca gene showed non-specific expression with relatively high levels of mrna accumulation in the roots, stems and leaves of a. thaliana. the prxea gene, on the other hand, accumulated high levels of mrna only in roots. promoter fragments from each gene were fused to the coding region of β-glucuronidase (gusa) reporter gene introduced into tobacco. promoter/gusa contructs were transferr ... | 1994 | 24193636 |
| relationship between transcript production and virus resistance in transgenic tobacco expressing the potato leafroll virus coat protein gene. | the coat protein (cp) gene of potato leafroll luteovims (plrv) was inserted into tobacco (nicotiana tabacum) using disarmed agrobacterium tumefaciens (lba4404) containing a binary expression vector. plrv cp gene transcript was detected in transgenic plants but its abundance differed between transformed lines. cp was not detected in virus-free transgenic plants. the segregation of kanamycin resistance in s1 seedling progenies (obtained by selfing transformed plants) indicated that multiple (up to ... | 1993 | 24196184 |
| transgenic plants of ramie (boehmeria nivea gaud.) obtained by agrobacterium mediated transformation. | a regeneration and transformation protocol for ramie (boehmeria nivea gaud.) is presented. regeneration was obtained from leaf discs placed on solid b-5 medium (gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. co-cultivation of leaf discs with agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by southern blot and analysis of expression of the gus-marker gene. | 1993 | 24201876 |
| effect of acetone in the culture medium on the regeneration efficiency and on the resistance to root rot of regenerants of various plant species. | various expiants of wheat, onion, garlic, and cabbage were examined for their regeneration efficiency in the presence of acetone in the culture medium. acetone at concentrations between 1.5% and 3.0% (w/v) was shown to increase the degree of callusing and meristem formation, as well as the capacity to regenerate. it also significantly increased the survival and regeneration efficiency of expiants inoculated with agrobacterium tumefaciens, a bacterium known to reduce expiant viability. resistance ... | 1993 | 24201789 |
| acetosyringone and osmoprotectants like betaine or proline synergistically enhance agrobacterium-mediated transformation of apple. | the effects of the plant signal molecule acetosyringone (as) and the osmoprotectant betaine phosphate (bp) have been examined for their ability to increase the transformation efficiency of agrobacterium tumefaciens (at), c58c1::pgv3850 harboring the binary vector pkiwi105. this binary plasmid encodes the β-glucuronidase (gus) gene and was previously shown to be expressed exclusively in plant tissues. bacteria were grown in one of two previously reported virulence induction media (ms20 and sim) f ... | 1993 | 24201785 |
| field performance of derived generations of transgenic tobacco. | two inbred cultivars of nicotiana tabacum (tobacco), 'samsun' and 'xanthi', were transformed with the plasmid pbi 121 using bin 19 in agrobacterium tumefaciens. the plasmid carries the nptii gene conferring kanamycin resistance and the uida gene encoding β-glucuronidase (gus). progeny carrying the genes in the homozygous condition were identified and selfed over several generations. one line homozygous for the introduced genes and one untransformed control from each cultivar were then selected a ... | 1993 | 24193884 |
| enhanced transformation of tomato co-cultivated with agrobacterium tumefaciens c58c1rif(r)::pgsfr1161 in the presence of acetosyringone. | explants of tomato (lycopersicon esculentum mill cv. ailsa craig) were co-cultivated with agrobacterium tumefaciens c58c1rif(r)::pgsfr1161 in the presence of 20 (μm acetosyringone). transformed root clones were selected on kanamycin medium and the presence of the nptii gene in the plant dna confirmed by the polymerase chain reaction. root clones derived from acetosyringone treatment grew more vigorously in the presence of kanamycin and synthesized a greater amount of npt-ii enzyme. the conclusio ... | 1993 | 24197345 |
| a modified method for routine agrobacterium-mediated transformation of in vitro grown potato microtubers. | in vitro-grown potato (solanum tuberosum l.) microtubers were used as an explant source in the production of transgenic plants by agrobacterium-mediated gene transfer. in this study we tested four diverse potato cultivars, lemhi russet, russet burbank, wauseon, and yankee chipper on various levels of zeatin riboside and 3-indoleacetyl-dl-aspartic acid for their ability to regenerate transgenic plants after infection with agrobacterium tumefaciens. culturing microtuber blocks from the medullary a ... | 1993 | 24197257 |
| transformation of liquidambar styraciflua using agrobacterium tumefaciens. | we describe the molecular transformation of liquidambar styraciflua using agrobacterium tumefaciens. a binary ti-plasmid vector containing a chimeric neomycin phosphotransferagene which confers resistance to kanamycin and either a chimeric bacillus thuringiensis toxin gene, a chimeric e. coli β-glucuronida(gus), or a chimeric tobacco anionic peroxidase gene was introduced into sweetgum by co-cultivation with agrobacterium tumefaciens. sweetgum shoots regenerated in the presence of kanamycin were ... | 1993 | 24197252 |
| nuclear genomic composition of asymmetric fusion products between irradiated transgenic solanum brevidens and s. tuberosum: limited elimination of donor chromosomes and polyploidization of the recipient genome. | the production of asymmetric somatic hybrid calli after fusion between gamma-irradiated protoplasts from transgenic solanum brevidens and protoplasts from s. tuberosum are reported. transgenic (kanamycin-resistant, gus-positive) s. brevidens plants and hairy root clones were obtained after transformation with agrobacterium tumefaciens lba 1060 (pri1855) (pbi121) and lba 4404 (pral4404) (pbi121), and a. rhizogenes lba 9402 (pri1855) (pbi121), respectively. leaf protoplasts isolated from the trans ... | 1993 | 24193466 |
| transgenic papaya plants from agrobacterium-mediated transformation of somatic embryos. | transgenic papaya (carica papaya l.) plants were regenerated from embryogenic cultures that were cocultivated with a disarmed c58 strain of agrobacterium tumefaciens containing one of the following binary cosmid vectors: pga482gg or pga482gg/cpprv-4. the t-dna region of both binary vectors includes the chimeric genes for neomycin phosphotransferase ii (nptii) and ß-glucuronidase (gus). in addition, the plant expressible coat protein (cp) gene of papaya ringspot virus (prv) is flanked by the npti ... | 1993 | 24197150 |
| genetic transformation of green bean callus via agrobacterium mediated dna transfer. | kanamycin resistant callus was produced from leaf disc or hypocotyl expiants of green bean (phaseolus vulgaris l.) when cultured on a defined medium containing 50 mg/l kanamycin after 4 days of co-cultivation with agrobacterium tumefaciens strain eha101 containing the binary vector pkylx71gus. the presence of neomycin phosphotransferase ii (npt-ii) in crude cellular extracts from the kanamycin resistant callus was confirmed by elisa. the expression of the ß-glucuronidase (gus) reporter gene was ... | 1993 | 24202072 |
| metabolic factors capable of inducing agrobacterium vir gene expression are present in rice (oryza sativa l.). | the effects of exudates and extracts from suspension cultures or various parts of rice (oryza sativa l.) plants on induction of vir (virulence) gene expression in agrobacterium tumefaciens were examined. only leaf extracts from panicle-differentiating plants to flowering plants were able to strongly induce activation and expression of vir genes. this induction was similar to that observed with 2 μm acetosyringone (as), yet there was no synergy between as and rice extracts. responses to vir-induc ... | 1993 | 24196854 |
| agrobacterium-mediated transformation of asparagus officinalis l. long-term embryogenic callus and regeneration of transgenic plants. | twenty-three independent kanamycin resistant lines were obtained after cocultivation of longterm embryogenic cultures of three asparagus officinalis l. genotypes with an agrobacterium tumefaciens strain harboring ß-glucuronidase and neomycin phosphotransferase ii genes. all the lines showed ß-glucuronidase activity by histological staining. dna analysis by southern blots of the kanamycin resistant embryogenic lines and of a plant regenerated from one of them confirmed the integration of the t-dn ... | 1993 | 24196848 |
| agrobacterium tumefaciens-mediated transformation of safflower (carthamus tinctorius l.) cv. 'centennial'. | efficient callus formation was achieved from cotyledon, stem, and leaf expiants of the domestic safflower cultivar 'centennial' on ms salts medium containing 1 mg/l bap and 1 mg/l naa. shoot buds were regenerated from 26% of leaf-derived calli on callus induction medium, although attempts to root regenerated shoots were not successful. 'centennial' expiants inoculated with agrobacterium tumefaciens containing npt ii and gus genes produced kanamycin-resistant calli from which buds were regenerate ... | 1992 | 24213291 |
| transformation and regeneration of brassica rapa using agrobacterium tumefaciens. | transformation and regeneration procedures for obtaining transgenic brassica rapa ssp. oleifera plants are described. regeneration frequencies were increasedby using silver nitrate and by adjusting the duration of exposure to 2,4-d. for transformation, agrobacterium tumefaciens strain eha101 containing a binary plasmid with the neomycin phosphotransferase gene (npt ii) and the b-glucuronidase gene (gus) was cocultivated with hypocotyl explants from the oilseed b. rapa cvs. tobin and emma. transf ... | 1992 | 24213157 |
| auxin autonomy in cultured tobacco teratoma tissues transformed by an auxin-mutant strain of agrobacterium tumefaciens. | we have studied the mechanism of auxin autonomy in tobacco (nicotiana tabacum l.) crowngall tissues transformed by the auxin-mutant (tms (-)) a66 strain of agrobacterium tumefaciens. normally, tms (-) tobacco tumor tissues require the formation of shoots to exhibit auxin-independent growth in culture. we have isolated from tms (-) tobacco cells several stable variants that are fully hormone-independent and grow rapidly as friable, unorganized tissues, thus mimicking the growth and morphology of ... | 1992 | 24178208 |
| inheritance of a bacterial hygromycin phosphotransferase gene in the progeny of primary transgenic pea plants. | an analysis of the progeny of primary transgenic pea plants in terms of transmission of the transferred dna, fertility and morphology is presented. a transformation system developed for pea that allows the regeneration of fertile transgenic pea plants from calli selected for antibiotic resistance was used. expiants from axenic shoot cultures were co-cultivated with a nononcogenic agrobacterium tumefaciens strain carrying a gene encoding hygromycin phosphotransferase as selectable marker, and tra ... | 1992 | 24203206 |
| shoot regeneration in stem expiants and its amenability to agrobacterium tumefaciens mediated gene transfer in brassica carinata. | immature stem segments of seven different genotypes of brassica carinata produced shoots with variable frequencies when cultured in ms medium with bap and picloram at 0.2 mg/l each. line 171, which produced shoots with 100% efficiency from both cut ends of the expiant, was selected for testing the amenability of this regeneration protocol for genetic transformation. a non-oncogenic agrobacterium tumefaciens containing plasmid pcv 730, a binary vector carrying resistance genes for kanamycin and h ... | 1992 | 24201439 |
| agrobacterium tumefaciens-mediated beta-glucuronidase (gus) gene expression in lentil (lens culinaris medik.) tissues. | lentil (lens culinaris medik.) shoot apex, epicotyl, and root expiants were capable of expressing an intron-containing beta-glucuronidase (gus) gene after inoculation with the disarmed agrobacterium strain gv2260:p35sgusint. expression occurred at all wound sites on these expiants except at the end of the root expiants proximal to the cotyledonary node. gus expression was detected using both histochemical and fluorescence assays and was stable for at least nine days after inoculation for epicoty ... | 1992 | 24203139 |
| agrobacterium mediated transfer of a mutant arabidopsis acetolactate synthase gene confers resistance to chlorsulfuron in chicory (cichorium intybus l.). | leaf discs of c. intybus were inoculated with an agrobacterium tumefaciens strain harboring a neomycin phosphotransferase (neo) gene for kanamycin resistance and a mutant acetolactate synthase gene (csr1-1) from arabidopsis thaliana conferring resistance to sulfonylurea herbicides. a regeneration medium was optimized which permitted an efficient shoot regeneration from leaf discs. transgenic shoots were selected on rooting medium containing 100 mg/l kanamycin sulfate. integration of the csr1-1 g ... | 1992 | 24203132 |
| factors influencing the efficiency of t-dna transfer during co-cultivation of antirrhinum majus with agrobacterium tumefaciens. | the effects of varying the ph of the cocultivation medium, additons of vir-inducing phenolic compounds and the strains of wild-type agrobacteria on transformation rates of a number of different varieties of antirrhinum majus were studied. in general, optimal transformation was found with strains c58 or a281 and was favoured by low ph and the inclusion of acetosyringone in the co-cultivation medium. however, maximal transformation of the least susceptible variety was achieved at high ph and in th ... | 1992 | 24202985 |
| transformation of vitis tissue by different strains of agrobacterium tumefaciens containing the t-6b gene. | stem pieces and leaf disks of vitis spp. were cocultured with agrobacterium tumefaciens strains carrying the uida (ß-glucuronidase = gus) gene. the transformation efficiency was highly increased by using a modified t-6b gene (a gene from ptitm4) which interferes with normal growth and allows regeneration of normal nicotiana rustica plants (tinland 1990). the strains first tested on stem segments were subsequently tested in a leaf explant system. on leaves the transformation efficiency of the str ... | 1992 | 24202984 |
| expression of soybean-embryo lipoxygenase 2 in transgenic tobacco tissue. | to assess the role of lipoxygenase (lox; ec 1.13.11.12) in plants, we increased the expression of lox in the tissues of nicotiana tabacum l. cv. 'ky 14' by over-expression of the lox2 gene from the soybean (glycine max (l.) merrill) embryo. the lox2 cdna was manipulated by replacing its 5'-untranslated sequence with the translational enhancer of the alfalfa mosaic virus (amv), and subcloned into a plant expression vector, 3' to a duplicated cauliflower mosaic virus 35s promoter. the amv-lox2 con ... | 1992 | 24178044 |
| transgenic poplars: expression of chimeric genes using four different constructs. | leaf or stem explants of a hybrid poplar clone (populus tremula x populus alba), sensitive to agrobacterium tumefaciens, were co-cultivated either by an octopine or a nopaline disarmed a. tumefaciens modified strain. transformed poplar shoots were readily regenerated from explants. the protocol was improved using the nopaline disarmed strain c58/pmp90 with the binary vector pbi121. this protocol was then used to test three other vectors. the first one, possessing a nptii gene fused to the camv 1 ... | 1992 | 24213546 |
| cucumber mosaic virus-tolerant transgenic tomato plants expressing a satellite rna. | a satellite rna (t73-satrna) gave reduced symptom severity on tomato plants when coinoculated with an ordinary strain of cucumber mosaic virus (cmvo). cdna for t73-satrna was introduced into a binary vector (ptok162) through a homologous recombination in an agrobacterium tumefaciens cell, and then transferred to leaf disks of tomato. stable integration and transcription of the cdna in the regenerants were verified by southern and northern blot hybridizations, respectively. upon inoculation with ... | 1992 | 24202740 |
| expression of pvx coat protein gene under the control of extensin-gene promoter confers virus resistance on transgenic potato plants. | tuber discs of potato (solanum tuberosum l.) cultivars desirée and gracia were infected by agrobacterium tumefaciens carrying a binary vector with the coat protein gene of potato virus x controlled by the carrot extensin gene long-transcript promoter. several transgenic potato plants have been obtained by direct regeneration of shoots on culture medium with kanamycin used for selection. the presence of the coat protein gene was proved by southern hybridization in several transformants. its low b ... | 1992 | 24213037 |
| coat protein mediated resistance to plum pox virus in nicotiana clevelandii and n. benthamiana. | transgenic nicotiana benthamiana and n. clevelandii plants expressing the coat protein of plum pox virus under the control of the 35s promoter from cauliflower mosaic virus were engineered by agrobacterium tumefaciens mediated transformation. the phenomenon of virus resistance was observed at different levels when transgenic plants, expressing the coat protein and control plants were compared after challenge infection with plum pox virus. n. clevelandii coat protein transgenic plants circumvent ... | 1992 | 24213033 |
| regeneration of transgenic plants of prunus armeniaca containing the coat protein gene of plum pox virus. | a system was developed which allows the transfer of foreign genes into apricot cultivars. we report the transformation and regeneration of prunus armeniaca plants with agrobacterium tumefaciens strain lba 4404 containing various binary plasmids, pbingusint, carrying the marker gene ß-glucuronidase (gus) and pbinppvm, carrying the coat protein gene of plum pox virus (ppv). the marker gene gus was used for optical evaluation of the efficiency of the transformation system. the coat protein gene of ... | 1992 | 24213032 |
| introduction and differential use of various promoters in pollen grains of nicotiana glutinosa and lilium longiflorum. | as part of our research to develop an alternative system for the transformation of recalcitrant plant species we investigated the use of the male gametophyte as a transformation vector. therefore the activity of four different promoters (camv 35s, lat52, chia pa2 and tr2') was analyzed in pollen of a dicot (nicotiana glutinosa) and a monocot (lilium longiflorum) plant species. gene constructs in which the ß-glucuronidase (gus) gene was placed under the control of these promoters were introduced ... | 1992 | 24213031 |
| transformation of solanum integrifolium poir via agrobacterium tumefaciens: plant regeneration and progeny analysis. | the wild species solanum integrifolium represents a source of pest and disease resistance genes for breeding strategies of the cultivated species solanum melongena. somatic hybridization via protoplast fusion between the two species may provide a valuable tool for transferring polygenic traits into the cultivated species. the availability of s.integrifolium cells carrying dominant selectable markers would facilitate the heterokaryon rescue. an appropriate methodology for in vitro culture and pla ... | 1992 | 24213029 |
| biosynthesis, processing and targeting of the g-protein of vesicular stomatitis virus in tobacco protoplasts. | leaf protoplasts of tobacco (nicotlana tabacum l.) were employed for transfection of chimeric transcriptional gene fusions comprising the 35s promoter from cauliflower mosaic virus, the coding sequence of the g-protein from vesicular stomatitis virus (vsvg) and the transcriptional terminator from the agrobacterium tumefaciens nopaline-synthetase gene. transient expression of the chimeric gene was monitored through northern analysis of total protoplast rna using a labeled vsv cdna probe, and thro ... | 1992 | 24186728 |
| patterns of transformation intensity on flax hypocotyls inoculated with agrobacterium tumefaciens. | cellular transformation intensities on flax (linum usitatissimum) hypocotyl explants using disarmed agrobacterium tumefaciens were investigated through various preculture durations, cocultivation durations and removal of epidermis. the expression of an intron-containing β-glucuronidase (gus) gene driven by camv 35s promoter served as a reporter for determination of transformed tissues on hypocotyls. the binary plasmid p35sgusint in octopine-type agrobacterium strain gv2260 was used as the vector ... | 1991 | 24221329 |
| induction of virulence response in agrobacterium tumefaciens by tissue explants of various plant species. | forty-four plant species belonging to different taxa were tested for their ability to induce the expression of the virulence e gene in agrobacterium tumefaciens containing vire:lacz fusion constructs. with the exception of 6 algae, one fern and 2 monocots, tissue explants of all other plants (2 algae, 3 bryophytes, 2 pteridophytes, 15 gymnosperms, 8 monocots and 5 dicots) induced the expression of the vire gene as detected by the presence of β-galactosidase activity in the bacteria. | 1991 | 24221327 |
| crown gall transformation of lentil (lens culinaris medik.) with virulent strains of agrobacterium tumefaciens. | four diverse strains of agrobacterium tumefaciens (c58, ach5, gv3111, and a281) were capable of inducing tumors at a high frequency on inoculated stems of lentil (lens culinaris medik. cultivar laird) in vivo, and on excised shoot apices in vitro. gv3111 and ach5 produced the largest and heaviest tumors in vivo, while a281 produced the heaviest tumors in vitro. tumor formation and opine production are indicative of plant cell transformation and tumors produced appropriate opines: nopaline (c58), ... | 1991 | 24221280 |
| growth and disinfestation of 6 different bacteria in embryogenic suspension cultures of cotton. | growth of 6 different common laboratory bacteria (escherichia coli, flavobacterium balustrum, xanthomonas maltophilia, enterobacter cloacae, pseudomonas fluorescens, and agrobacterium tumefaciens) in a bacterial medium, fresh plant medium, and "spent" plant media was initially measured. in all cases, bacteria grew best in the bacterial medium followed by the fresh plant medium. the spent plant medium did not support growth of the bacteria and apparently was actively toxic to bacterial cells. pro ... | 1991 | 24221727 |
| gene transfer into peanut (arachis hypogaea l.) by agrobacterium tumefaciens. | introduction of foreign genes into plant tissues via agrobacterium tumefaciens based vectors requires specific knowledge of agrobacterium-host compatibility. therefore, to develop a transformation protocol for peanut (arachis hypogaea l.), five brazilian cultivars were screened with four wild-type a.tumefaciens strains. successful transformation was dependent on specific bacterial strain-plant cultivar interactions and strain a281 was the most effective for tumor induction. tumors displayed horm ... | 1991 | 24221673 |