| unravelling the structural and molecular basis responsible for the anti-biofilm activity of zosteric acid. | the natural compound zosteric acid, or p-(sulfoxy)cinnamic acid (za), is proposed as an alternative biocide-free agent suitable for preventive or integrative anti-biofilm approaches. despite its potential, the lack of information concerning the structural and molecular mechanism of action involved in its anti-biofilm activity has limited efforts to generate more potent anti-biofilm strategies. in this study a 43-member library of small molecules based on za scaffold diversity was designed and sc ... | 2015 | 26132116 |
| transcription factor-based biosensors enlightened by the analyte. | whole cell biosensors (wcbs) have multiple applications for environmental monitoring, detecting a wide range of pollutants. wcbs depend critically on the sensitivity and specificity of the transcription factor (tf) used to detect the analyte. we describe the mechanism of regulation and the structural and biochemical properties of tf families that are used, or could be used, for the development of environmental wcbs. focusing on the chemical nature of the analyte, we review tfs that respond to ar ... | 2015 | 26191047 |
| delicate conformational balance of the redox enzyme cytochrome p450cam. | the energy landscapes of proteins are highly complex and can be influenced by changes in physical and chemical conditions under which the protein is studied. the redox enzyme cytochrome p450cam undergoes a multistep catalytic cycle wherein two electrons are transferred to the heme group and the enzyme visits several conformational states. using paramagnetic nmr spectroscopy with a lanthanoid tag, we show that the enzyme bound to its redox partner, putidaredoxin, is in a closed state at ambient t ... | 2015 | 26130807 |
| epidemiology and characteristics of metallo-β-lactamase-producing pseudomonas aeruginosa. | metallo-β-lactamase-producing pseudomonas aeruginosa (mppa) is an important nosocomial pathogen that shows resistance to all β-lactam antibiotics except monobactams. there are various types of metallo-β-lactamases (mbls) in carbapenem-resistant p. aeruginosa including imipenemase (imp), verona integron-encoded metallo-β-lactamase (vim), sao paulo metallo-β-lactamase (spm), germany imipenemase (gim), new delhi metallo-β-lactamase (ndm), florence imipenemase (fim). each mbl gene is located on spec ... | 2015 | 26157586 |
| differential molecular response of monodehydroascorbate reductase and glutathione reductase by nitration and s-nitrosylation. | the ascorbate-glutathione cycle is a metabolic pathway that detoxifies hydrogen peroxide and involves enzymatic and non-enzymatic antioxidants. proteomic studies have shown that some enzymes in this cycle such as ascorbate peroxidase (apx), monodehydroascorbate reductase (mdar), and glutathione reductase (gr) are potential targets for post-translational modifications (pmts) mediated by nitric oxide-derived molecules. using purified recombinant pea peroxisomal mdar and cytosolic and chloroplastic ... | 2015 | 26116026 |
| genome, proteome and structure of a t7-like bacteriophage of the kiwifruit canker phytopathogen pseudomonas syringae pv. actinidiae. | pseudomonas syringae pv. actinidiae is an economically significant pathogen responsible for severe bacterial canker of kiwifruit (actinidia sp.). bacteriophages infecting this phytopathogen have potential as biocontrol agents as part of an integrated approach to the management of bacterial canker, and for use as molecular tools to study this bacterium. a variety of bacteriophages were previously isolated that infect p. syringae pv. actinidiae, and their basic properties were characterized to pro ... | 2015 | 26114474 |
| metabolism of doubly para-substituted hydroxychlorobiphenyls by bacterial biphenyl dioxygenases. | in this work, we examined the profile of metabolites produced from the doubly para-substituted biphenyl analogs 4,4'-dihydroxybiphenyl, 4-hydroxy-4'-chlorobiphenyl, 3-hydroxy-4,4'-dichlorobiphenyl, and 3,3'-dihydroxy-4,4'-chlorobiphenyl by biphenyl-induced pandoraea pnomenusa b356 and by its biphenyl dioxygenase (bpdo). 4-hydroxy-4'-chlorobiphenyl was hydroxylated principally through a 2,3-dioxygenation of the hydroxylated ring to generate 2,3-dihydro-2,3,4-trihydroxy-4'-chlorobiphenyl and 3,4-d ... | 2015 | 25956777 |
| enterocin f4-9, a novel o-linked glycosylated bacteriocin. | enterococcus faecalis f4-9 isolated from egyptian salted-fermented fish produces a novel bacteriocin, termed enterocin f4-9. enterocin f4-9 was purified from the culture supernatant by three steps, and its molecular mass was determined to be 5,516.6 da by mass spectrometry. amino acid and dna sequencing showed that the propeptide consists of 67 amino acid residues, with a leader peptide containing a double glycine cleavage site to produce a 47-amino-acid mature peptide. enterocin f4-9 is modifie ... | 2015 | 25956765 |
| the regulatory function of lexa is temperature-dependent in the deep-sea bacterium shewanella piezotolerans wp3. | the sos response addresses dna lesions and is conserved in the bacterial domain. the response is governed by the dna binding protein lexa, which has been characterized in model microorganisms such as escherichia coli. however, our understanding of its roles in deep-sea bacteria is limited. here, the influence of lexa on the phenotype and gene transcription of shewanella piezotolerans wp3 (wp3) was investigated by constructing a lexa deletion strain (wp3δlexa), which was compared with the wild-ty ... | 2015 | 26150814 |
| production of novel nan3-resistant creatine amidinohydrolase in recombinant escherichia coli. | creatinase (creatine amidinohydrolase), an important medical enzyme, has been used for clinical diagnosis of renal function because of its high substrate specificity. recently, we successfully cloned a nan3-resistant creatinase encoding gene from arthrobacter nicotianae. by optimizing the cultivation process, we realized its high-level expression in escherichia coli. in this addendum, production of this nan3-resistant creatinase in e. coli and future research were further discussed. | 2015 | 26083084 |
| a macrophage subversion factor is shared by intracellular and extracellular pathogens. | pathogenic bacteria have developed strategies to adapt to host environment and resist host immune response. several intracellular bacterial pathogens, including salmonella enterica and mycobacterium tuberculosis, share the horizontally-acquired mgtc virulence factor that is important for multiplication inside macrophages. mgtc is also found in pathogenic pseudomonas species. here we investigate for the first time the role of mgtc in the virulence of an extracellular pathogen, pseudomonas aerugin ... | 2015 | 26080006 |
| complete genome sequence of the cyanogenic phosphate-solubilizing pseudomonas sp. strain ccos 191, a close relative of pseudomonas mosselii. | we sequenced the complete genome of the isolate pseudomonas sp. ccos 191. this strain is able to dissolve phosphate minerals and form cyanide. the genome sequence is used to establish the phylogenetic relationship of this species. | 2015 | 26067963 |
| finding optimal interaction interface alignments between biological complexes. | biological molecules perform their functions through interactions with other molecules. structure alignment of interaction interfaces between biological complexes is an indispensable step in detecting their structural similarities, which are key s: to understanding their evolutionary histories and functions. although various structure alignment methods have been developed to successfully access the similarities of protein structures or certain types of interaction interfaces, existing alignment ... | 2015 | 26072475 |
| artificial de novo biosynthesis of hydroxystyrene derivatives in a tyrosine overproducing escherichia coli strain. | styrene and its derivatives as monomers and petroleum-based feedstocks are valuable as raw materials in industrial processes. the chemical reaction for styrene production uses harsh reaction conditions such as high temperatures or pressures, or requires base catalysis with microwave heating. on the other hand, production of styrene and its derivatives in escherichia coli is an environmental friendly process to produce conventional petroleum-based feedstocks. | 2015 | 26055892 |
| functional characterization of salicylate hydroxylase from the fungal endophyte epichloë festucae. | epichloë spp. are symbiotic fungal endophytes of many cool season grasses. the presence of the fungal endophytes often confers insect, drought, and disease tolerance to the host grasses. the presence of the fungal endophytes within the host plants does not elicit host defense responses. the molecular basis for this phenomenon is not known. epichloë festucae, the endophyte of festuca rubra, expresses a salicylate hydroxylase similar to nahg from the bacterium pseudomonas putida. few fungal salicy ... | 2015 | 26055188 |
| photoautotrophic polyhydroxybutyrate granule formation is regulated by cyanobacterial phasin phap in synechocystis sp. strain pcc 6803. | cyanobacteria are photoautotrophic microorganisms which fix atmospheric carbon dioxide via the calvin-benson cycle to produce carbon backbones for primary metabolism. fixed carbon can also be stored as intracellular glycogen, and in some cyanobacterial species like synechocystis sp. strain pcc 6803, polyhydroxybutyrate (phb) accumulates when major nutrients like phosphorus or nitrogen are absent. so far only three enzymes which participate in phb metabolism have been identified in this organism, ... | 2015 | 25911471 |
| draft genome sequence of hydrocarbon-degrading pseudomonas putida strain kg-4, isolated from soil samples collected from krishna-godavari basin in india. | we report here the 5.58-mb draft genome of pseudomonas putida strain kg-4 obtained from the oil fields of the krishna-godavari basin, andhra pradesh, india. the genome sequence is expected to facilitate identification and understanding of genes associated with hydrocarbon metabolism, which can help in developing strategies for managing oil spills and bioremediation. | 2015 | 26044433 |
| the design of simple bacterial microarrays: development towards immobilizing single living bacteria on predefined micro-sized spots on patterned surfaces. | in this paper we demonstrate a procedure for preparing bacterial arrays that is fast, easy, and applicable in a standard molecular biology laboratory. microcontact printing is used to deposit chemicals promoting bacterial adherence in predefined positions on glass surfaces coated with polymers known for their resistance to bacterial adhesion. highly ordered arrays of immobilized bacteria were obtained using microcontact printed islands of polydopamine (pd) on glass surfaces coated with the antia ... | 2015 | 26039378 |
| effect of low-density static magnetic field on the oxidation of ammonium by nitrosomonas europaea and by activated sludge in municipal wastewater. | ammonium removal is a key step in biological wastewater treatment and novel approaches that improve this process are in great demand. the aim of this study is to test the hypothesis that ammonium removal from wastewater can be stimulated by static magnetic fields. this was achieved by analysis of the effects of static magnetic field (smf) on the growth and activity of nitrosomonas europaea, a key ammonia-oxidising bacterium, where increased growth and increased ammonia oxidation rate were detect ... | 2015 | 27904349 |
| psychrotrophic bacteria in milk: how much do we really know? | the occurrence of psychrotrophic bacteria in raw milk is studied worldwide due to the difficulties associated with controlling their growth during cold storage and the consequent negative effects upon fluid milk or dairy products. among the psychrotrophic bacteria, the genus pseudomonas (represented primarily by p. fluorescens) has been highlighted as the cause of numerous defects in dairy products. in light of its perceived predominance, this species has frequently been chosen as a model organi ... | 2015 | 26273245 |
| the wrky45-dependent signaling pathway is required for resistance against striga hermonthica parasitism. | the root hemiparasite witchweed (striga spp.) is a devastating agricultural pest that causes losses of up to $1 billion us annually in sub-saharan africa. development of resistant crops is one of the cost-effective ways to address this problem. however, the molecular mechanisms underlying resistance are not well understood. to understand molecular events upon striga spp. infection, we conducted genome-scale rna sequencing expression analysis using striga hermonthica-infected rice (oryza sativa) ... | 2015 | 26025049 |
| high-throughput screening for a moderately halophilic phenol-degrading strain and its salt tolerance response. | a high-throughput screening system for moderately halophilic phenol-degrading bacteria from various habitats was developed to replace the conventional strain screening owing to its high efficiency. bacterial enrichments were cultivated in 48 deep well microplates instead of shake flasks or tubes. measurement of phenol concentrations was performed in 96-well microplates instead of using the conventional spectrophotometric method or high-performance liquid chromatography (hplc). the high-throughpu ... | 2015 | 26020478 |
| hierarchy of carbon source utilization in soil bacteria: hegemonic preference for benzoate in complex aromatic compound mixtures degraded by cupriavidus pinatubonensis strain jmp134. | cupriavidus pinatubonensis jmp134, like many other environmental bacteria, uses a range of aromatic compounds as carbon sources. previous reports have shown a preference for benzoate when this bacterium grows on binary mixtures composed of this aromatic compound and 4-hydroxybenzoate or phenol. however, this observation has not been extended to other aromatic mixtures resembling a more archetypal context. we carried out a systematic study on the substrate preference of c. pinatubonensis jmp134 g ... | 2015 | 25795675 |
| markers of oxidative stress and neuroprogression in depression disorder. | major depression is multifactorial disorder with high prevalence and alarming prognostic in the nearest 15 years. several mechanisms of depression are known. neurotransmitters imbalance and imbalance between neuroprogressive and neuroprotective factors are observed in major depression. depression is accompanied by inflammatory responses of the organism and consequent elevation of proinflammatory cytokines and increased lipid peroxidation are described in literature. neuropsychiatric disorders in ... | 2015 | 26078821 |
| two decades of warming increases diversity of a potentially lignolytic bacterial community. | as earth's climate warms, the massive stores of carbon found in soil are predicted to become depleted, and leave behind a smaller carbon pool that is less accessible to microbes. at a long-term forest soil-warming experiment in central massachusetts, soil respiration and bacterial diversity have increased, while fungal biomass and microbially-accessible soil carbon have decreased. here, we evaluate how warming has affected the microbial community's capability to degrade chemically-complex soil c ... | 2015 | 26042112 |
| draft genome sequence of pseudomonas abietaniphila kf701 (nbrc110664), a polychlorinated biphenyl-degrading bacterium isolated from biphenyl-contaminated soil. | pseudomonas abietaniphila kf701 utilizes biphenyl as a sole source of carbon and degrades polychlorinated biphenyls (pcbs). here, we report the 6,886,250-bp draft genome sequence of kf701, which contains 6,315 coding sequences and 59.4 mol% g+c content. the strain possesses genes for biphenyl catabolism and other genes that mediate the degradation of benzoate, salicylate, and phenol. | 2015 | 25977441 |
| three pseudomonas putida fnr family proteins with different sensitivities to o2. | the escherichia coli fumarate-nitrate reduction regulator (fnr) protein is the paradigm for bacterial o2-sensing transcription factors. however, unlike e. coli, some bacterial species possess multiple fnr proteins that presumably have evolved to fulfill distinct roles. here, three fnr proteins (anr, pp_3233, and pp_3287) from a single bacterial species, pseudomonas putida kt2440, have been analyzed. under anaerobic conditions, all three proteins had spectral properties resembling those of [4fe-4 ... | 2015 | 25971977 |
| marine systems biology. | | 2015 | 26029243 |
| lov-based optogenetic devices: light-driven modules to impart photoregulated control of cellular signaling. | the light-oxygen-voltage domain family of proteins is widespread in biology where they impart sensory responses to signal transduction domains. the small, light responsive lov modules offer a novel platform for the construction of optogenetic tools. currently, the design and implementation of these devices is partially hindered by a lack of understanding of how light drives allosteric changes in protein conformation to activate diverse signal transduction domains. further, divergent photocycle p ... | 2015 | 25988185 |
| identification of metabolism pathways directly regulated by sigma(54) factor in bacillus thuringiensis. | sigma(54) (σ(54)) regulates nitrogen and carbon utilization in bacteria. promoters that are σ(54)-dependent are highly conserved and contain short sequences located at the -24 and -12 positions upstream of the transcription initiation site. σ(54) requires regulatory proteins known as bacterial enhancer-binding proteins (bebps) to activate gene transcription. we show that σ(54) regulates the capacity to grow on various nitrogen sources using a bacillus thuringiensis hd73 mutant lacking the sigl g ... | 2015 | 26029175 |
| what it takes to be a pseudomonas aeruginosa? the core genome of the opportunistic pathogen updated. | pseudomonas aeruginosa is an opportunistic bacterial pathogen able to thrive in highly diverse ecological niches and to infect compromised patients. its genome exhibits a mosaic structure composed of a core genome into which accessory genes are inserted en bloc at specific sites. the size and the content of the core genome are open for debate as their estimation depends on the set of genomes considered and the pipeline of gene detection and clustering. here, we redefined the size and the content ... | 2015 | 25961859 |
| review of micro/nanotechnologies for microbial biosensors. | a microbial biosensor is an analytical device with a biologically integrated transducer that generates a measurable signal indicating the analyte concentration. this method is ideally suited for the analysis of extracellular chemicals and the environment, and for metabolic sensory regulation. although microbial biosensors show promise for application in various detection fields, some limitations still remain such as poor selectivity, low sensitivity, and impractical portability. to overcome such ... | 2015 | 26029689 |
| contribution of a low-barrier hydrogen bond to catalysis is not significant in ketosteroid isomerase. | low-barrier hydrogen bonds (lbhbs) have been proposed to have important influences on the enormous reaction rate increases achieved by many enzymes. δ(5)-3-ketosteroid isomerase (ksi) catalyzes the allylic isomerization of δ(5)-3-ketosteroid to its conjugated δ(4)-isomers at a rate that approaches the diffusion limit. tyr14, a catalytic residue of ksi, has been hypothesized to form an lbhb with the oxyanion of a dienolate steroid intermediate generated during the catalysis. the unusual chemical ... | 2015 | 25947291 |
| enhanced horizontal transfer of antibiotic resistance genes in freshwater microcosms induced by an ionic liquid. | the spread and propagation of antibiotic resistance genes (args) is a worldwide public health concern. ionic liquids (ils), considered as "environmentally friendly" replacements for industrial organic solvents, have been widely applied in modern industry. however, few data have been collected regarding the potential ecological and environmental risks of ils, which are important for preparing for their potential discharge into the environment. in this paper, the il 1-butyl-3-methylimidazolium hex ... | 2015 | 25951456 |
| cosmetics-triggered percutaneous remote control of transgene expression in mice. | synthetic biology has significantly advanced the rational design of trigger-inducible gene switches that program cellular behavior in a reliable and predictable manner. capitalizing on genetic componentry, including the repressor pmer and its cognate operator opmer, that has evolved in pseudomonas syringae pathovar tomato dc3000 to sense and resist plant-defence metabolites of the paraben class, we have designed a set of inducible and repressible mammalian transcription-control devices that coul ... | 2015 | 25943548 |
| re-evaluation of dioxygenase gene phylogeny for the development and validation of a quantitative assay for environmental aromatic hydrocarbon degraders. | rieske non-heme iron oxygenases enzymes have been widely studied, as they catalyse essential reactions initiating the bacterial degradation of organic compounds, for instance aromatic hydrocarbons. the genes encoding these enzymes offer a potential target for studying aromatic hydrocarbon-degrading organisms in the environment. however, previously reported primer sets that target dioxygenase gene sequences or the common conserved rieske centre of aromatics dioxygenases have limited specificity a ... | 2015 | 25944871 |
| quantitatively predicting bacterial adhesion using surface free energy determined with a spectrophotometric method. | bacterial adhesion onto solid surfaces is of importance in a wide spectrum of problems, including environmental microbiology, biomedical research, and various industrial applications. despite many research efforts, present thermodynamic models that rely on the evaluation of the adhesion energy are often elusive in predicting the bacterial adhesion behavior. here, we developed a new spectrophotometric method to determine the surface free energy (sfe) of bacterial cells. the adhesion behaviors of ... | 2015 | 25898026 |
| dephosphorylated npr is involved in an envelope stress response of escherichia coli. | besides the canonical phosphoenolpyruvate-dependent phosphotransferase system (pts) for carbohydrate transport, most proteobacteria possess the so-called nitrogen pts (pts(ntr)) that transfers a phosphate group from phosphoenolpyruvate (pep) over enzyme i(ntr) (ei(ntr)) and npr to enzyme iia(ntr) (eiia(ntr)). the pts(ntr) lacks membrane-bound components and functions exclusively in a regulatory capacity. while eiia(ntr) has been implicated in a variety of cellular processes such as potassium hom ... | 2015 | 25701731 |
| reactions of cg10062, a cis-3-chloroacrylic acid dehalogenase homologue, with acetylene and allene substrates: evidence for a hydration-dependent decarboxylation. | cg10062 is a cis-3-chloroacrylic acid dehalogenase (cis-caad) homologue from corynebacterium glutamicum with an unknown function and an uninformative genomic context. it shares 53% pairwise sequence similarity with cis-caad including the six active site amino acids (pro-1, his-28, arg-70, arg-73, tyr-103, and glu-114) that are critical for cis-caad activity. however, cg10062 is a poor cis-caad: it lacks catalytic efficiency and isomer specificity. two acetylene compounds (propiolate and 2-butyno ... | 2015 | 25894805 |
| multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from pseudomonas aeruginosa pao1 as revealed by global phenotypic analysis. | pseudomonas aeruginosa is known to be a multidrug resistant opportunistic pathogen. particularly, p. aeruginosa pao1 polyphosphate kinase mutant (ppk1) is deficient in motility, quorum sensing, biofilm formation and virulence. | 2015 | 25907584 |
| immobilized laccase mediated dye decolorization and transformation pathway of azo dye acid red 27. | laccases have good potential as bioremediating agents and can be used continuously in the immobilized form like many other enzymes. | 2015 | 25932329 |
| characterization of uronate dehydrogenases catalysing the initial step in an oxidative pathway. | uronate dehydrogenases catalyse the oxidation of uronic acids to aldaric acids, which represent 'top value-added chemicals' that have the potential to substitute petroleum-derived chemicals. the identification and annotation of three uronate dehydrogenases derived from fulvimarina pelagi htcc2506, streptomyces viridochromogenes dsm 40736 and oceanicola granulosus dsm 15982 via sequence analysis is described. characterization and comparison with two known uronate dehydrogenases in regard to subst ... | 2015 | 25884328 |
| the crystal structure of d-threonine aldolase from alcaligenes xylosoxidans provides insight into a metal ion assisted plp-dependent mechanism. | threonine aldolases catalyze the pyridoxal phosphate (plp) dependent cleavage of threonine into glycine and acetaldehyde and play a major role in the degradation of this amino acid. in nature, l- as well as d-specific enzymes have been identified, but the exact physiological function of d-threonine aldolases (dtas) is still largely unknown. both types of enantio-complementary enzymes have a considerable potential in biocatalysis for the stereospecific synthesis of various β-hydroxy amino acids, ... | 2015 | 25884707 |
| bioaugmentation of mesorhizobium cicer, pseudomonas spp. and piriformospora indica for sustainable chickpea production. | chickpea establishes symbiotic association with mesorhizobium to fulfill its nitrogen (n) requirement. integrating chickpea rhizosphere with potential native mesorhizobia and other plant growth promoting microorganisms can contribute multiple benefits to plants. the present investigation was undertaken to study interactions among piriformospora indica (pi) with potential plant growth promoting rhizobacteria (pgpr) viz. pseudomonas argentinensis (lpgpr1), pseudomonas sp. (lpgpr2) along with natio ... | 2015 | 26261403 |
| isolation and characterization of polyacrylamide-degrading bacteria from dewatered sludge. | polyacrylamide (pam) is a water-soluble polymer that is widely used as a flocculant in sewage treatment. the accumulation of pam affects the formation of dewatered sludge and potentially produces hazardous monomers. in the present study, the bacterial strain hi47 was isolated from dewatered sludge. this strain could metabolize pam as its sole nutrient source and was subsequently identified as pseudomonas putida. the efficiency of pam degradation was 31.1% in 7 days and exceeded 45% under optimum ... | 2015 | 25893998 |
| draft genome sequence of lysinibacillus fusiformis strain sw-b9, a novel strain for biotransformation of isoeugenol to vanillin. | lysinibacillus fusiformis sw-b9 was the first reported strain in l. fusiformis showing effective biotransformation of isoeugenol to vanillin. here, we report the annotated genome of strain sw-b9, which has special pathways for producing vanillin. the genome will provide a genetic basis for better understanding the physiology of this species. | 2015 | 25883291 |
| marine hydrocarbonoclastic bacteria as whole-cell biosensors for n-alkanes. | whole-cell biosensors offer potentially useful, cost-effective systems for the in-situ monitoring of seawater for hydrocarbons derived from accidental spills. the present work compares the performance of a biosensor system for the detection of alkanes in seawater, hosted in either escherichia coli (commonly employed in whole-cell biosensors but not optimized for alkane assimilation) or different marine bacteria specialized in assimilating alkanes. the sensor system was based on the pseudomonas p ... | 2015 | 25874658 |
| biphenyl 4-hydroxylases involved in aucuparin biosynthesis in rowan and apple are cytochrome p450 736a proteins. | upon pathogen attack, fruit trees such as apple (malus spp.) and pear (pyrus spp.) accumulate biphenyl and dibenzofuran phytoalexins, with aucuparin as a major biphenyl compound. 4-hydroxylation of the biphenyl scaffold, formed by biphenyl synthase (bis), is catalyzed by a cytochrome p450 (cyp). the biphenyl 4-hydroxylase (b4h) coding sequence of rowan (sorbus aucuparia) was isolated and functionally expressed in yeast (saccharomyces cerevisiae). sab4h was named cyp736a107. no catalytic function ... | 2015 | 25862456 |
| conjunctival flora of clinically normal and diseased turtles and tortoises. | in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises. | 2015 | 25889261 |
| engineering mediator-based electroactivity in the obligate aerobic bacterium pseudomonas putida kt2440. | pseudomonas putida strains are being developed as microbial production hosts for production of a range of amphiphilic and hydrophobic biochemicals. p. putida's obligate aerobic growth thereby can be an economical and technical challenge because it requires constant rigorous aeration and often causes reactor foaming. here, we engineered a strain of p. putida kt2440 that can produce phenazine redox-mediators from pseudomonas aeruginosa to allow partial redox balancing with an electrode under oxyge ... | 2015 | 25914687 |
| cell surface-based sensing with metallic nanoparticles. | metallic nanoparticles provide versatile scaffolds for biosensing applications. in this review, we focus on the use of metallic nanoparticles for cell surface sensings. examples of the use of both specific recognition and array-based "chemical nose" approaches to cell surface sensing will be discussed. | 2015 | 25853985 |
| comparative ser/thr/tyr phosphoproteomics between two mycobacterial species: the fast growing mycobacterium smegmatis and the slow growing mycobacterium bovis bcg. | ser/thr/tyr protein phosphorylation plays a critical role in regulating mycobacterial growth and development. understanding the mechanistic link between protein phosphorylation signaling network and mycobacterial growth rate requires a global view of the phosphorylation events taking place at a given time under defined conditions. in the present study we employed a phosphopeptide enrichment and high throughput mass spectrometry-based strategy to investigate and qualitatively compare the phosphop ... | 2015 | 25904896 |
| cysb negatively affects the transcription of pqsr and pseudomonas quinolone signal production in pseudomonas aeruginosa. | pseudomonas aeruginosa is a gram-negative bacterium that is ubiquitous in the environment, and it is an opportunistic pathogen that can infect a variety of hosts, including humans. during the process of infection, p. aeruginosa coordinates the expression of numerous virulence factors through the production of multiple cell-to-cell signaling molecules. the production of these signaling molecules is linked through a regulatory network, with the signal n-(3-oxododecanoyl) homoserine lactone and its ... | 2015 | 25845844 |
| panoramic view of a superfamily of phosphatases through substrate profiling. | large-scale activity profiling of enzyme superfamilies provides information about cellular functions as well as the intrinsic binding capabilities of conserved folds. herein, the functional space of the ubiquitous haloalkanoate dehalogenase superfamily (hadsf) was revealed by screening a customized substrate library against >200 enzymes from representative prokaryotic species, enabling inferred annotation of ∼35% of the hadsf. an extremely high level of substrate ambiguity was revealed, with the ... | 2015 | 25848029 |
| antibiotic-free selection in biotherapeutics: now and forever. | the continuously improving sophistication of molecular engineering techniques gives access to novel classes of bio-therapeutics and new challenges for their production in full respect of the strengthening regulations. among these biologic agents are dna based vaccines or gene therapy products and to a lesser extent genetically engineered live vaccines or delivery vehicles. the use of antibiotic-based selection, frequently associated with genetic manipulation of microorganism is currently undergo ... | 2015 | 25854922 |
| prediction of distal residue participation in enzyme catalysis. | a scoring method for the prediction of catalytically important residues in enzyme structures is presented and used to examine the participation of distal residues in enzyme catalysis. scores are based on the partial order optimum likelihood (pool) machine learning method, using computed electrostatic properties, surface geometric features, and information obtained from the phylogenetic tree as input features. predictions of distal residue participation in catalysis are compared with experimental ... | 2015 | 25627867 |
| crystal structure of x-prolyl aminopeptidase from caenorhabditis elegans: a cytosolic enzyme with a di-nuclear active site. | eukaryotic aminopeptidase p1 (app1), also known as x-prolyl aminopeptidase (xpnpep1) in human tissues, is a cytosolic exopeptidase that preferentially removes amino acids from the n-terminus of peptides possessing a penultimate n-terminal proline residue. the enzyme has an important role in the catabolism of proline containing peptides since peptide bonds adjacent to the imino acid proline are resistant to cleavage by most peptidases. we show that recombinant and catalytically active caenorhabdi ... | 2015 | 25905034 |
| draft genome sequence of pseudomonas sp. nov. h2. | we report the draft genome sequence of pseudomonas sp. nov. h2, isolated from creek sediment in moscow, id, usa. the strain is most closely related to pseudomonas putida. however, it has a slightly smaller genome that appears to have been impacted by horizontal gene transfer and poorly maintains incp-1 plasmids. | 2015 | 25838493 |
| occurrence of potentially pathogenic bacterial-endosymbionts in acanthamoeba spp. | acanthamoeba- bacteria interactions enable pathogenic bacteria to tolerate harsh conditions and lead to transmission to the susceptible host. the present study was aimed to address the presence of bacterial endosymbionts of acanthamoeba isolated from recreational water sources of tehran, iran. to the best of our knowledge this is the first study regarding occurrence of bacteria in environmental acanthamoeba spp. in iran. | 2015 | 26246815 |
| role of cyanobacterial exopolysaccharides in phototrophic biofilms and in complex microbial mats. | exopolysaccharides (epss) are an important class of biopolymers with great ecological importance. in natural environments, they are a common feature of microbial biofilms, where they play key protective and structural roles. as the primary colonizers of constrained environments, such as desert soils and lithic and exposed substrates, cyanobacteria are the first contributors to the synthesis of the epss constituting the extracellular polymeric matrix that favors the formation of microbial associa ... | 2015 | 25837843 |
| characterization and structure prediction of partial length protein sequences of pcoa, pcor and chrb genes from heavy metal resistant bacteria from the klip river, south africa. | the klip river has suffered from severe anthropogenic effects from industrial activities such as mining. long-term exposure to heavy metal pollution has led to the development of heavy metal resistant strains of pseudomonas sp. kr23, lysinibacillus sp. kr25, and e. coli kr29. the objectives of this study were to characterize the genetics of copper and chromate resistance of the isolates. copper and chromate resistance determinants were cloned and sequenced. open reading frames (orfs) related to ... | 2015 | 25837632 |
| the glycerol-dependent metabolic persistence of pseudomonas putida kt2440 reflects the regulatory logic of the glpr repressor. | the growth of the soil bacterium pseudomonas putida kt2440 on glycerol as the sole carbon source is characterized by a prolonged lag phase, not observed with other carbon substrates. we examined the bacterial growth in glycerol cultures while monitoring the metabolic activity of individual cells. fluorescence microscopy and flow cytometry, as well as the analysis of the temporal start of growth in single-cell cultures, revealed that adoption of a glycerol-metabolizing regime was not the result o ... | 2015 | 25827416 |
| plant-microbe cross-talk in the rhizosphere: insight and biotechnological potential. | rhizosphere, the interface between soil and plant roots, is a chemically complex environment which supports the development and growth of diverse microbial communities. the composition of the rhizosphere microbiome is dynamic and controlled by multiple biotic and abiotic factors that include environmental parameters, physiochemical properties of the soil, biological activities of the plants and chemical signals from the plants and bacteria which inhabit the soil adherent to root-system. recent a ... | 2015 | 25926899 |
| role of bacillus subtilis dna glycosylase mutm in counteracting oxidatively induced dna damage and in stationary-phase-associated mutagenesis. | reactive oxygen species (ros) promote the synthesis of the dna lesion 8-oxo-g, whose mutagenic effects are counteracted in distinct organisms by the dna glycosylase mutm. we report here that in bacillus subtilis, mutm is expressed during the exponential and stationary phases of growth. in agreement with this expression pattern, results of a western blot analysis confirmed the presence of mutm in both stages of growth. in comparison with cells of a wild-type strain, cells of b. subtilis lacking m ... | 2015 | 25825434 |
| an s188v mutation alters substrate specificity of non-stereospecific α-haloalkanoic acid dehalogenase e (dehe). | the non-stereospecific α-haloalkanoic acid dehalogenase e (dehe) degrades many halogenated compounds but is ineffective against β-halogenated compounds such as 3-chloropropionic acid (3cp). using molecular dynamics (md) simulations and site-directed mutagenesis we show here that introducing the mutation s188v into dehe improves substrate specificity towards 3cp. md simulations showed that residues w34, f37, and s188 of dehe were crucial for substrate binding. dehe showed strong binding ability f ... | 2015 | 25816329 |
| biodegradation of naphthalene using pseudomonas aeruginosa by up flow anoxic-aerobic continuous flow combined bioreactor. | naphthalene is a poly aromatic hydrocarbon (pah) present in many sediment-water systems. the aim of this study was to evaluate the applicability of an anoxic/aerobic system for the biological treatment of water polluted by naphthalene by pseudomonas aeruginosa ptcc 1707 to utilize naphthalene. the naphthalene elimination from wastewater was determined in anoxic-aerobic continuous flow combined bioreactor under continuously oxic and anoxic conditions. experiments were conducted in continues mode, ... | 2015 | 25859393 |
| effects of three different nucleoid-associated proteins encoded on incp-7 plasmid pcar1 on host pseudomonas putida kt2440. | nucleoid-associated proteins (naps), which fold bacterial dna and influence gene transcription, are considered to be global transcriptional regulators of genes on both plasmids and the host chromosome. incompatibility p-7 group plasmid pcar1 carries genes encoding three naps: h-ns family protein pmr, ndpa-like protein pnd, and hu-like protein phu. in this study, the effects of single or double disruption of pmr, pnd, and phu were assessed in host pseudomonas putida kt2440. when pmr and pnd or pm ... | 2015 | 25681185 |
| arsenic methylation and volatilization by arsenite s-adenosylmethionine methyltransferase in pseudomonas alcaligenes nbrc14159. | inorganic arsenic (as) is highly toxic and ubiquitous in the environment. inorganic as can be transformed by microbial methylation, which constitutes an important part of the as biogeochemical cycle. in this study, we investigated as biotransformation by pseudomonas alcaligenes nbrc14159. p. alcaligenes was able to methylate arsenite [as(iii)] rapidly to dimethylarsenate and small amounts of trimethylarsenic oxide. an arsenite s-adenosylmethionine methyltransferase, paarsm, was identified and fu ... | 2015 | 25681184 |
| engineering protocells: prospects for self-assembly and nanoscale production-lines. | the increasing ease of producing nucleic acids and proteins to specification offers potential for design and fabrication of artificial synthetic "organisms" with a myriad of possible capabilities. the prospects for these synthetic organisms are significant, with potential applications in diverse fields including synthesis of pharmaceuticals, sources of renewable fuel and environmental cleanup. until now, artificial cell technology has been largely restricted to the modification and metabolic eng ... | 2015 | 25815781 |
| the intracellular scots pine shoot symbiont methylobacterium extorquens dsm13060 aggregates around the host nucleus and encodes eukaryote-like proteins. | endophytes are microbes that inhabit plant tissues without any apparent signs of infection, often fundamentally altering plant phenotypes. while endophytes are typically studied in plant roots, where they colonize the apoplast or dead cells, methylobacterium extorquens strain dsm13060 is a facultatively intracellular symbiont of the meristematic cells of scots pine (pinus sylvestris l.) shoot tips. the bacterium promotes host growth and development without the production of known plant growth-st ... | 2015 | 25805725 |
| characterization of kfra proteins encoded by a plasmid of paenibacillus popilliae atcc 14706(t). | a scaffold obtained from whole-genome shotgun sequencing of paenibacillus popilliae atcc 14706(t) shares partial homology with plasmids found in other strains of p. popilliae. pcr and sequencing for gap enclosure indicated that the scaffold originated from a 15,929-bp circular dna. the restriction patterns of a plasmid isolated from p. popilliae atcc 14706(t) were identical to those expected from the sequence; thus, this circular dna was identified as a plasmid of atcc 14706(t) and designated pp ... | 2015 | 25853059 |
| procalcitonin levels in gram-positive, gram-negative, and fungal bloodstream infections. | procalcitonin (pct) can discriminate bacterial from viral systemic infections and true bacteremia from contaminated blood cultures. the aim of this study was to evaluate pct diagnostic accuracy in discriminating gram-positive, gram-negative, and fungal bloodstream infections. a total of 1,949 samples from patients with suspected bloodstream infections were included in the study. median pct value in gram-negative (13.8 ng/ml, interquartile range (iqr) 3.4-44.1) bacteremias was significantly highe ... | 2015 | 25852221 |
| interdomain hydrophobic interactions modulate the thermostability of microbial esterases from the hormone-sensitive lipase family. | microbial hormone-sensitive lipases (hsls) contain a cap domain and a catalytic domain. however, it remains unclear how the cap domain interacts with the catalytic domain to maintain the stability of microbial hsls. here, we isolated an hsl esterase, e40, from a marine sedimental metagenomic library. e40 exhibited the maximal activity at 45 °c and was quite thermolabile, with a half-life of only 2 min at 40 °c, which may be an adaptation of e40 to the permanently cold sediment environment. the s ... | 2015 | 25771540 |
| crystal structure of the n-acetylmuramic acid α-1-phosphate (murnac-α1-p) uridylyltransferase muru, a minimal sugar nucleotidyltransferase and potential drug target enzyme in gram-negative pathogens. | the n-acetylmuramic acid α-1-phosphate (murnac-α1-p) uridylyltransferase muru catalyzes the synthesis of uridine diphosphate (udp)-murnac, a crucial precursor of the bacterial peptidoglycan cell wall. muru is part of a recently identified cell wall recycling pathway in gram-negative bacteria that bypasses the general de novo biosynthesis of udp-murnac and contributes to high intrinsic resistance to the antibiotic fosfomycin, which targets udp-murnac de novo biosynthesis. to provide insights into ... | 2015 | 25767118 |
| biobutanol from cheese whey. | at present, due to environmental and economic concerns, it is urgent to evolve efficient, clean and secure systems for the production of advanced biofuels from sustainable cheap sources. biobutanol has proved better characteristics than the more widely used bioethanol, however the main disadvantage of biobutanol is that it is produced in low yield and titer by abe (acetone-butanol-ethanol) fermentation, this process being not competitive from the economic point of view. in this review we summari ... | 2015 | 25889728 |
| strain and process development for poly(3hb-co-3hp) fermentation by engineered shimwellia blattae from glycerol. | poly(3-hydroxybytyrate-co-3-hydroxypropionate), poly(3hb-co-3hp), is a possible alternative to synthetic polymers such as polypropylene, polystyrene and polyethylene due to its low crystallinity and fragility. we already reported that recombinant strains of shimwellia blattae expressing 1,3-propanediol dehydrogenase dhat as well as aldehyde dehydrogenase aldd of pseudomonas putida kt2442, propionate-coa transferase pct of clostridium propionicum x2 and pha synthase phac1 of ralstonia eutropha h1 ... | 2015 | 25852995 |
| a novel arabinose-inducible genetic operation system developed for clostridium cellulolyticum. | clostridium cellulolyticum and other cellulolytic clostridium strains are natural producers of lignocellulosic biofuels and chemicals via the consolidated bioprocessing (cbp) route, and systems metabolic engineering is indispensable to meet the cost-efficient demands of industry. several genetic tools have been developed for clostridium strains, and an efficient and stringent inducible genetic operation system is still required for the precise regulation of the target gene function. | 2015 | 25763107 |
| proteomics as the final step in the functional metagenomics study of antimicrobial resistance. | the majority of clinically applied antimicrobial agents are derived from natural products generated by soil microorganisms and therefore resistance is likely to be ubiquitous in such environments. this is supported by the fact that numerous clinically important resistance mechanisms are encoded within the genomes of such bacteria. advances in genomic sequencing have enabled the in silico identification of putative resistance genes present in these microorganisms. however, it is not sufficient to ... | 2015 | 25784907 |
| a reusable immobilization matrix for the biodegradation of phenol at 5000 mg/l. | bacteria-mediated degradation of toxins has been reported as a practical technique for the innocuous removal of toxic organic compounds from water. specifically, immobilized and pre-acclimatized pseudomonas putida has been shown to clear low levels of contaminants (less than 2000 mg/l) from wastewater, wherein the bacteria consumes toxic aromatic compounds as the only source of carbon and energy. here we report the preparation of a high-capacity composite adsorbent as an immobilization matrix fo ... | 2015 | 25733015 |
| in-office use of a steroid-eluting implant for maintenance of frontal ostial patency after revision sinus surgery. | achieving long-term, successful outcomes with endoscopic sinus surgery (ess) can be challenging in patients with recalcitrant chronic rhinosinusitis (crs). local complications, including scar formation and ostial stenosis, can lead to recurrent blockage and subsequent relapse. the frontal sinus is particularly vulnerable to surgical failure given its narrow outflow and inaccessibility to topical therapies. the advent of steroid-eluting sinus implants has enhanced ess outcomes, with significant r ... | 2015 | 25668577 |
| isolation of ndm-1-producing multidrug-resistant pseudomonas putida from a paediatric case of acute gastroenteritis, india. | pseudomonas putida is an uncommon opportunistic pathogen, usually susceptible to antimicrobial agents. data concerning resistance to antimicrobial agents in clinical p. putida isolates are limited. to the best of our knowledge we report for the first time the isolation of ndm-1-producing multidrug-resistant p. putida from a case of acute gastroenteritis. the isolate showed resistance to a wide range of antimicrobials, including fluoroquinolones, third-generation cephalosporins and carbapenems. t ... | 2015 | 25893095 |
| gene mdpc plays a regulatory role in the methyl-tert-butyl ether degradation pathway of methylibium petroleiphilum strain pm1. | among the few bacteria known to utilize methyl tert-butyl ether (mtbe) as a sole carbon source, methylibium petroleiphilum pm1 is a well-characterized organism with a sequenced genome; however, knowledge of the genetic regulation of its mtbe degradation pathway is limited. we investigated the role of a putative transcriptional activator gene, mdpc, in the induction of mtbe-degradation genes mdpa (encoding mtbe monooxygenase) and mdpj (encoding tert-butyl alcohol hydroxylase) of strain pm1 in a g ... | 2015 | 25724531 |
| rhizosphere microbial community composition affects cadmium and zinc uptake by the metal-hyperaccumulating plant arabidopsis halleri. | the remediation of metal-contaminated soils by phytoextraction depends on plant growth and plant metal accessibility. soil microorganisms can affect the accumulation of metals by plants either by directly or indirectly stimulating plant growth and activity or by (im)mobilizing and/or complexing metals. understanding the intricate interplay of metal-accumulating plants with their rhizosphere microbiome is an important step toward the application and optimization of phytoremediation. we compared t ... | 2015 | 25595759 |
| comparative genome analysis reveals the molecular basis of nicotine degradation and survival capacities of arthrobacter. | arthrobacter is one of the most prevalent genera of nicotine-degrading bacteria; however, studies of nicotine degradation in arthrobacter species remain at the plasmid level (plasmid pao1). here, we report the bioinformatic analysis of a nicotine-degrading arthrobacter aurescens m2012083, and show that the moeb and moga genes that are essential for nicotine degradation in arthrobacter are absent from plasmid pao1. homologues of all the nicotine degradation-related genes of plasmid pao1 were foun ... | 2015 | 25721465 |
| expression of extra-cellular levansucrase in pseudomonas syringae is controlled by the in planta fitness-promoting metabolic repressor hexr. | pseudomonas syringae pv. glycinea pg4180 causes bacterial blight on soybean plants and enters the leaf tissue through stomata or open wounds, where it encounters a sucrose-rich milieu. sucrose is utilized by invading bacteria via the secreted enzyme, levansucrase (lsc), liberating glucose and forming the polyfructan levan. p. syringae pg4180 possesses two functional lsc alleles transcribed at virulence-promoting low temperatures. | 2015 | 25886911 |
| structural studies on laz, a promiscuous anticancer neisserial protein. | azurin and laz (lipidated azurin) are 2 bacterial proteins with anticancer, anti-viral and anti-parasitic activities. azurin, isolated from the bacterium pseudomonas aeruginosa, termed paz, demonstrates anticancer activity against a range of cancers but not against brain tumors. in contrast, laz is produced by members of gonococci/meningococci, including neisseria meningitides which can cross the blood-brain barrier to infect brain meninges. it has been previously reported that laz has an additi ... | 2015 | 25714335 |
| an event-driven approach for studying gene block evolution in bacteria. | gene blocks are genes co-located on the chromosome. in many cases, gene blocks are conserved between bacterial species, sometimes as operons, when genes are co-transcribed. the conservation is rarely absolute: gene loss, gain, duplication, block splitting and block fusion are frequently observed. an open question in bacterial molecular evolution is that of the formation and breakup of gene blocks, for which several models have been proposed. these models, however, are not generally applicable to ... | 2015 | 25717195 |
| genome reduction boosts heterologous gene expression in pseudomonas putida. | the implementation of novel platform organisms to be used as microbial cell factories in industrial applications is currently the subject of intense research. ongoing efforts include the adoption of pseudomonas putida kt2440 variants with a reduced genome as the functional chassis for biotechnological purposes. in these strains, dispensable functions removed include flagellar motility (1.1% of the genome) and a number of open reading frames expected to improve genotypic and phenotypic stability ... | 2015 | 25890048 |
| natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools. | genetically encoded optical tools have revolutionized modern biology by allowing detection and control of biological processes with exceptional spatiotemporal precision and sensitivity. natural photoreceptors provide researchers with a vast source of molecular templates for engineering of fluorescent proteins, biosensors, and optogenetic tools. here, we give a brief overview of natural photoreceptors and their mechanisms of action. we then discuss fluorescent proteins and biosensors developed fr ... | 2015 | 25706899 |
| core principles of bacterial autoinducer systems. | autoinduction (ai), the response to self-produced chemical signals, is widespread in the bacterial world. this process controls vastly different target functions, such as luminescence, nutrient acquisition, and biofilm formation, in different ways and integrates additional environmental and physiological cues. this diversity raises questions about unifying principles that underlie all ai systems. here, we suggest that such core principles exist. we argue that the general purpose of ai systems is ... | 2015 | 25694124 |
| engineering sugar utilization and microbial tolerance toward lignocellulose conversion. | production of fuels and chemicals through a fermentation-based manufacturing process that uses renewable feedstock such as lignocellulosic biomass is a desirable alternative to petrochemicals. although it is still in its infancy, synthetic biology offers great potential to overcome the challenges associated with lignocellulose conversion. in this review, we will summarize the identification and optimization of synthetic biological parts used to enhance the utilization of lignocellulose-derived s ... | 2015 | 25741507 |
| promotion of arsenic phytoextraction efficiency in the fern pteris vittata by the inoculation of as-resistant bacteria: a soil bioremediation perspective. | a greenhouse pot experiment was carried out to evaluate the efficiency of arsenic phytoextraction by the fern pteris vittata growing in arsenic-contaminated soil, with or without the addition of selected rhizobacteria isolated from the polluted site. the bacterial strains were selected for arsenic resistance, the ability to reduce arsenate to arsenite, and the ability to promote plant growth. p. vittata plants were cultivated for 4 months in a contaminated substrate consisting of arsenopyrite ci ... | 2015 | 25741356 |
| microbial community successional patterns in beach sands impacted by the deepwater horizon oil spill. | although petroleum hydrocarbons discharged from the deepwater horizon (dwh) blowout were shown to have a pronounced impact on indigenous microbial communities in the gulf of mexico, effects on nearshore or coastal ecosystems remain understudied. this study investigated the successional patterns of functional and taxonomic diversity for over 1 year after the dwh oil was deposited on pensacola beach sands (fl, usa), using metagenomic and 16s rrna gene amplicon techniques. gamma- and alphaproteobac ... | 2015 | 25689026 |
| a single serine residue determines selectivity to monovalent metal ions in metalloregulators of the merr family. | merr metalloregulators alleviate toxicity caused by an excess of metal ions, such as copper, zinc, mercury, lead, cadmium, silver, or gold, by triggering the expression of specific efflux or detoxification systems upon metal detection. the sensor protein binds the inducer metal ion by using two conserved cysteine residues at the c-terminal metal-binding loop (mbl). divalent metal ion sensors, such as merr and zntr, require a third cysteine residue, located at the beginning of the dimerization (α ... | 2015 | 25691529 |
| single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming of penicillium chrysogenum. | the cholesterol-lowering blockbuster drug pravastatin can be produced by stereoselective hydroxylation of the natural product compactin. we report here the metabolic reprogramming of the antibiotics producer penicillium chrysogenum toward an industrial pravastatin production process. following the successful introduction of the compactin pathway into the β-lactam-negative p. chrysogenum ds50662, a new cytochrome p450 (p450 or cyp) from amycolatopsis orientalis (cyp105as1) was isolated to catalyz ... | 2015 | 25691737 |
| bioconversion of styrene to poly(hydroxyalkanoate) (pha) by the new bacterial strain pseudomonas putida nbus12. | styrene is a toxic pollutant commonly found in waste effluents from plastic processing industries. we herein identified and characterized microorganisms for bioconversion of the organic eco-pollutant styrene into a valuable biopolymer medium-chain-length poly(hydroxyalkanoate) (mcl-pha). twelve newly-isolated styrene-degrading pseudomonads were obtained and partial phac genes were detected by pcr in these isolates. these isolates assimilated styrene to produce mcl-pha, forming pha contents betwe ... | 2015 | 25740622 |
| microbiological characteristics of sepsis in a university hospital. | microbiological characteristics of sepsis and antimicrobial resistance are well studied, although in state university of campinas, no data has been published yet. | 2015 | 25887416 |
| soybean (glycine max l. merr.) sprouts germinated under red light irradiation induce disease resistance against bacterial rotting disease. | specific wavelengths of light can exert various physiological changes in plants, including effects on responses to disease incidence. to determine whether specific light wavelength had effects on rotting disease caused by pseudomonas putida 229, soybean sprouts were germinated under a narrow range of wavelengths from light emitting diodes (leds), including red (650-660), far red (720-730) and blue (440-450 nm) or broad range of wavelength from daylight fluorescence bulbs. the controls were compo ... | 2015 | 25679808 |
| green synthesis and characterization of carica papaya leaf extract coated silver nanoparticles through x-ray diffraction, electron microscopy and evaluation of bactericidal properties. | the evolution of nanotechnology and the production of nanomedicine from various sources had proven to be of intense value in the field of biomedicine. the smaller size of nanoparticles is gaining importance in research for the treatment of various diseases. moreover the production of nanoparticles is eco-friendly and cost effective. in the present study silver nanoparticles were synthesized from carica papaya leaf extract (cpl) and characterized for their size and shape using scanning electron m ... | 2015 | 26288570 |
| third generation cephalosporin resistant enterobacteriaceae and multidrug resistant gram-negative bacteria causing bacteremia in febrile neutropenia adult cancer patients in lebanon, broad spectrum antibiotics use as a major risk factor, and correlation with poor prognosis. | bacteremia remains a major cause of life-threatening complications in patients receiving anticancer chemotherapy. the spectrum and susceptibility profiles of causative microorganisms differ with time and place. data from lebanon are scarce. we aim at evaluating the epidemiology of bacteremia in cancer patients in a university hospital in lebanon, emphasizing antibiotic resistance and risk factors of multi-drug resistant organism (mdro)-associated bacteremia. | 2015 | 25729741 |