| activation of the chromosomally encoded mazef(bif) locus of bifidobacterium longum under acid stress. | toxin-antitoxin (ta) systems are distributed within the genomes of almost all free-living bacteria. although the roles of chromosomally encoded ta systems are still under debate, they are suspected to be involved in various stress responses. here, we provide the first report of a type ii ta system in the probiotic bacterium bifidobacterium longum. bioinformatic analysis of the b. longum jdm301 genome identified a pair of linked genes encoding a mazef-like ta system at the locus blj_811-blj_812. ... | 2015 | 25950853 |
| complete genome sequence of bifidobacterium longum gt15: identification and characterization of unique and global regulatory genes. | in this study, we report the first completely annotated genome sequence of the russia origin bifidobacterium longum subsp. longum strain gt15. comparative genomic analysis of this genome with other available completely annotated genome sequences of b. longum strains isolated from other countries has revealed a high degree of conservation and synteny across the entire genomes. however, it was discovered that the open reading frames to 35 genes were detected only from the b. longum gt15 genome and ... | 2015 | 25894918 |
| starch and starch hydrolysates are favorable carbon sources for bifidobacteria in the human gut. | bifidobacteria are key commensals in human gut, and their abundance is associated with the health of their hosts. although they are dominant in infant gut, their number becomes lower in adult gut. the changes of the diet are considered to be main reason for this difference. large amounts of whole-genomic sequence data of bifidobacteria make it possible to elucidate the genetic interpretation of their adaptation to the nutrient environment. among the nutrients in human gut, starch is a highly fer ... | 2015 | 25887661 |
| applications of microencapsulated bifidobacterium longum with eleutherine americana in fresh milk tofu and pineapple juice. | bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. microencapsulation of b. longum with eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. microencapsulated b. longum with the extract and oligosaccharides extract in the food products showed better sur ... | 2015 | 25854832 |
| effect of salt on cell viability and membrane integrity of lactobacillus acidophilus, lactobacillus casei and bifidobacterium longum as observed by flow cytometry. | the aim of the current study was to investigate the effect of varying sodium chloride concentrations (0-5%) on viability and membrane integrity of three probiotic bacteria, lactobacillus acidophilus, lactobacillus casei and bifidobacterium longum, using conventional technique and flow cytometry. double staining of cells by carboxyfluorescein diacetate (cfda) and propidium iodide (pi) enabled to evaluate the effect of nacl on cell esterase activity and membrane integrity. observations from conven ... | 2015 | 25846931 |
| effects of bifidobacterium supplementation on intestinal microbiota composition and the immune response in healthy infants. | intestinal microbiotas are thought to be the most important source of maturational stimuli to the development of the immune system. however, few studies have focused on the development of t helper (th) 1 immune response and antibody response to vaccinations in healthy infants, especially in a large cohort. through this randomized, double-blind control trial, we investigated the effects of bifidobacterium longum bb536 (bb536) supplementation on intestinal microbiota composition and the immune res ... | 2016 | 25846071 |
| overexpression of small heat shock protein enhances heat- and salt-stress tolerance of bifidobacterium longum ncc2705. | bifidobacteria are probiotics that are incorporated live into various dairy products. they confer health-promotive effects via gastrointestinal tract colonization. however, to provide their health-beneficial properties, they must battle the various abiotic stresses in that environment, such as bile salts, acids, oxygen, and heat. in this study, bifidobacterium longum salt- and heat-stress tolerance was enhanced by homologous overexpression of a small heat shock protein (shsp). a positive contrib ... | 2015 | 25842174 |
| complete genome sequence of bifidobacterium longum subsp. infantis strain cect 7210, a probiotic strain active against rotavirus infections. | bifidobacterium longum subsp. infantis cect 7210 is a probiotic strain able to inhibit rotavirus in vitro and protect against viral infection in both cell cultures and mice. here, we report its complete genome sequence, as deciphered by pacbio single-molecule real-time (smrt) technology. an analysis of the sequence may provide insights into its functional activity. | 2015 | 25838473 |
| bioaccessible antioxidants in milk fermented by bifidobacterium longum subsp. longum strains. | bifidobacterium longum subsp. longum is among the dominant species of the human gastrointestinal microbiota and could thus have potential as probiotics. new targets such as antioxidant properties have interest for beneficial effects on health. the objective of this study was to evaluate the bioaccessibility of antioxidants in milk fermented by selected b. longum subsp. longum strains during in vitro dynamic digestion. the antioxidant capacity of cell extracts from 38 strains, of which 32 belong ... | 2015 | 25802836 |
| chronic administration of a microencapsulated probiotic enhances the bioavailability of orange juice flavanones in humans. | orange juice (oj) flavanones are bioactive polyphenols that are absorbed principally in the large intestine. ingestion of probiotics has been associated with favorable changes in the colonic microflora. the present study examined the acute and chronic effects of orally administered bifidobacterium longum r0175 on the colonic microflora and bioavailability of oj flavanones in healthy volunteers. in an acute study volunteers drank oj with and without the microencapsulated probiotic, whereas the ch ... | 2015 | 25801290 |
| vagotomy prevents the effect of probiotics on caspase activity in a model of postmyocardial infarction depression. | myocardial infarction (mi) is associated with apoptosis in the amygdala and, ultimately, with clinical signs of depression. different treatments have proven to be beneficial in preventing depression, including combination of the probiotics lactobacillus helveticus and bifidobacterium longum for prophylaxis. we have speculated previously that the benefit of these probiotics is due to their anti-inflammatory properties, and evidence suggests that an intact vagus nerve is important for this effect ... | 2015 | 25786501 |
| analysis of gene expression of bifidobacteria using as the reporter an anaerobic fluorescent protein. | to determine the effectiveness of evoglow-pp1 as a reporter to study gene expression in bifidobacteria. to choose a strong and constitutive promoter to track fluorescently labelled bifidobacteria in environments under anaerobic conditions. | 2015 | 25778800 |
| bifidobacterium lemurum sp. nov., from faeces of the ring-tailed lemur (lemur catta). | four gram-positive-staining, microaerophilic, non-spore-forming, fructose-6-phosphate phosphoketolase-positive bacterial strains were isolated from a faecal sample of a 5-year-old ring-tailed lemur (lemur catta). the strains showed a peculiar morphology, resembling a small coiled snake, a ring shape, or forming a little 'y' shape. the isolated strains appeared identical, and lmc 13t was chosen as a representative strain and characterized further. strain lmc 13t showed an a3β peptidoglycan type, ... | 2015 | 25736415 |
| cocktails of probiotics pre-adapted to multiple stress factors are more robust under simulated gastrointestinal conditions than their parental counterparts and exhibit enhanced antagonistic capabilities against escherichia coli and staphylococcus aureus. | the success of the probiotics in delivery of health benefits depends on their ability to withstand the technological and gastrointestinal conditions; hence development of robust cultures is critical to the probiotic industry. combinations of probiotic cultures have proven to be more effective than the use of single cultures for treatment and prevention of heterogeneous diseases. we investigated the effect of pre- adaptation of probiotics to multiple stresses on their stability under simulated ga ... | 2015 | 25729438 |
| isolation and transposition properties of isblo11, an active insertion sequence belonging to the is3 family, from bifidobacterium longum 105-a. | transposon mutagenesis systems are still under development in bifidobacteria, partly because intrinsic active insertion sequences are not well characterized in bifidobacteria. here, we isolated an active insertion sequence, isblo11, from bifidobacterium longum 105-a using a sacb-based counterselection system, which is generally used to screen for active insertion sequences from bacterial genomes. isblo11 is 1432 bp long and belongs to the is3 family. it has a single orf encoding a transposase an ... | 2015 | 25724534 |
| effect of pre-stressing on the acid-stress response in bifidobacterium revealed using proteomic and physiological approaches. | weak acid resistance limits the application of bifidobacteria as a probiotic in food. the acid tolerance response (atr), caused by pre-stressing cells at a sublethal ph, could improve the acid resistance of bifidobacteria to subsequent acid stress. in this study, we used bifidobacterium longum sub. longum bbmn68 to investigate the effect of the atr on the acid stress response (asr), and compared the difference between the atr and the asr by analyzing the two-dimensional-page protein profiles and ... | 2015 | 25689631 |
| computing smallest intervention strategies for multiple metabolic networks in a boolean model. | this article considers the problem whereby, given two metabolic networks n1 and n2, a set of source compounds, and a set of target compounds, we must find the minimum set of reactions whose removal (knockout) ensures that the target compounds are not producible in n1 but are producible in n2. similar studies exist for the problem of finding the minimum knockout with the smallest side effect for a single network. however, if technologies of external perturbations are advanced in the near future, ... | 2015 | 25684199 |
| a engineered bifidobacterium longum secreting a bioative penetratin-glucagon-like peptide 1 fusion protein enhances glucagon-like peptide 1 absorption in the intestine. | glucagon-like peptide-1 (glp-1) is an incretin-derived hormone secreted by intestinal l-cells in response to nutrient ingestion. it improves pancreatic function and it is considered to have a potential role in treatment of type 2 diabetes. however, its short half-life limits its therapeutic applications. here, to circumvent this problem, we engineered bifidobacterium longum, a bacterium naturally occurring in the intestinal flora, to express a secreted and biologically active form of the human g ... | 2015 | 25674803 |
| open-close structural change upon ligand binding and two magnesium ions required for the catalysis of n-acetylhexosamine 1-kinase. | infant gut-associated bifidobacteria possess a metabolic pathway to utilize lacto-n-biose (gal-β1,3-glcnac) and galacto-n-biose (gal-β1,3-galnac) from human milk and glycoconjugates specifically. in this pathway, n-acetylhexosamine 1-kinase (nahk) catalyzes the phosphorylation of glcnac or galnac at the anomeric c1 position with atp. crystal structures of nahk have only been determined in the closed state. in this study, we determined open state structures of nahk in three different forms (apo, ... | 2015 | 25644306 |
| biodistribution of a promising probiotic, bifidobacterium longum subsp. longum strain bbmn68, in the rat gut. | bifidobacterium longum subsp. longum bbmn68, isolated from centenarians in guangxi, china, has been proved to be a promising probiotic strain for its health benefits. in this study, the biodistribution of this strain in the rat gut was first investigated using the quantitative realtime pcr assay and propidium monoazide. strain-specific primers were originally designed based on the bbmn68 genome sequence. healthy rats were orally inoculated with either a single dose of bbmn68 (10(10) colony-formi ... | 2015 | 25639716 |
| preparation of eleutherine americana-alginate complex microcapsules and application in bifidobacterium longum. | microencapsulation using extrusion and emulsion techniques was prepared for bifidobacterium longum protection against sequential exposure to simulated gastric and intestinal juices, refrigeration storage and heat treatment. eleutherine americana was used as the co-encapsulating agent. hydrolysis of e. americana by gastric and intestinal juices was also determined. e. americana and its oligosaccharide extract demonstrated their resistance to low ph and partial tolerance to human α-amylase. microe ... | 2015 | 25629556 |
| bacterial community structure associated with elective cesarean section versus vaginal delivery in chinese newborns. | increasing attention is being paid to the potential for cesarean birth to influence the taxa of the bacteria that compose the infant intestinal microbiota. the present study characterized the diversity of the intestinal microbiota in newborn infants delivered vaginally (vd) or by cesarean section (cd). | 2015 | 25625578 |
| sequencing analysis and characterization of the plasmid pbif10 isolated from bifidobacterium longum. | a resident plasmid, pbif10, was isolated from bifidobacterium longum b200304, and the full-length sequence of pbif10 was analyzed. in this sequence, we identified at least 17 major open reading frames longer than 200 bp. a tetracycline resistance gene, tetq, was identified and verified to confer antibiotic resistance to tetracycline. the plasmid replicon with replication protein b gene (repb) and a typical iteron was identified in pbif10. an artificial clone vector was constructed with the repli ... | 2015 | 25587774 |
| antimicrobial activity of human β-defensins against lactic acid bacteria. | in this study, we evaluated the antimicrobial activity of human β-defensin-1 (hbd-1), human β-defensin-2 (hbd-2) and human β-defensin-3 (hbd-3) against three internationally common probiotic strains of lactic acid bacterium. our results indicated that hbd-1, hbd-2 and hbd-3 at the range of 0.08-10 μg/ml do not have obvious antimicrobial activity against these strains. viability of bifidobacterium longum jdm301 (b. longum jdm301), bifidobacterium lactis hn019 (b. lactis hn019) and lactobacillus r ... | 2015 | 25560646 |
| molecular characterisation of abc-type multidrug efflux systems in bifidobacterium longum. | administration of probiotic bacteria such as bifidobacterium spp. can prevent antibiotic associated diarrhoea since they can survive the often harsh conditions of the gut. in bifidobacterium longum subsp. longum(t) ncimb 702259, two gene clusters, with homology to the atp-binding cassette (abc) family of efflux transporters, were identified and studied to assess their functional contribution to antibiotic resistance. both gene clusters contained two genes encoding putative efflux transporters an ... | 2015 | 25529295 |
| growth and safety evaluation of infant formulae containing oligosaccharides derived from bovine milk: a randomized, double-blind, noninferiority trial. | a limited number of nondigestible oligosaccharides are available for use in infant formula. this study evaluated growth and safety in infants fed formula supplemented with a mixture of bovine milk-derived oligosaccharides (bmos). this mixture, which was generated from whey permeate, contains galactooligosaccharides and other oligosaccharides from bovine milk, such as 3'- and 6'-sialyllactose. we hypothesized that growth in infants fed bmos-supplemented formula would be noninferior to that in inf ... | 2014 | 25527244 |
| complete genome sequence of bifidobacterium longum gt15: unique genes for russian strains. | in this study, we report the first completely annotated genome sequence of the russian-origin bifidobacterium longum subsp. longum strain gt15. we discovered 35 unique genes (ugs) which were detected from only the b. longum gt15 genome and were absent from other b. longum strain genomes (not of russian origin). | 2014 | 25523785 |
| complete genome sequence of bifidobacterium longum 105-a, a strain with high transformation efficiency. | bifidobacterium longum 105-a shows high transformation efficiency and allows for the generation of gene knockout mutants through homologous recombination. here, we report the complete genome sequence of strain 105-a. genes encoding at least four putative restriction-modification systems were found in this genome, which might contribute to its transformation efficiency. | 2014 | 25523770 |
| optimization of fermentation parameters to study the behavior of selected lactic cultures on soy solid state fermentation. | the use of solid fermentation substrate (ssf) has been appreciated by the demand for natural and healthy products. lactic acid bacteria and bifidobacteria play a leading role in the production of novel functional foods and their behavior is practically unknown in these systems. soy is an excellent substrate for the production of functional foods for their low cost and nutritional value. the aim of this work was to optimize different parameters involved in solid state fermentation (ssf) using sel ... | 2015 | 25498472 |
| bifidobacterium longum bacteremia in preterm infants receiving probiotics. | administration of probiotics to premature newborns has been shown to prevent necrotizing enterocolitis and reduce all-cause mortality. in our hospital, we documented 2 cases of bifidobacterium longum subspecies infantis bacteremia in newborns receiving probiotics. by comparative genomics, we confirmed that the strains isolated from each patient originated from the probiotics. | 2015 | 25472946 |
| probiotic bacteria, b. longum and l. acidophilus inhibit infection by rotavirus in vitro and decrease the duration of diarrhea in pediatric patients. | evidence suggests that specific probiotics may be antagonistic to enteric pathogens and enhance immunity, and thus, provide a means of preventing or treating diarrheal diseases. in the present study, we aimed to evaluate the efficacy of probiotic strains isolated from koreans for the treatment of viral gastroenteritis in young children and against rotavirus in vitro. | 2015 | 25459995 |
| multi-functional glycoside hydrolase: blon_0625 from bifidobacterium longum subsp. infantis atcc 15697. | we here describe a unique β-d-glucosidase (bgl; blon_0625) derived from bifidobacterium longum subsp. infantis atcc 15697. the blon_0625 gene was expressed by recombinant escherichia coli. purified recombinant blon_0625 retains hydrolyzing activity against both p-nitrophenyl-β-d-glucopyranoside (pnpg; 17.3±0.24umg(-1)) and p-nitrophenyl-β-d-xylopyranoside (pnpx; 16.7±0.32umg(-1)) at ph 6.0, 30°c. to best of our knowledge, no previously described bgl retains the same level of both pnpgase and pnp ... | 2015 | 25435500 |
| case series of bifidobacterium longum bacteremia in three preterm infants on probiotic therapy. | the use of probiotics as prophylaxis for necrotizing enterocolitis (nec) in preterm infants is being increasingly practised. | 2015 | 25402825 |
| consecutive oral administration of bifidobacterium longum mm-2 improves the defense system against influenza virus infection by enhancing natural killer cell activity in a murine model. | bifidobacterium, one of the major components of intestinal microflora, shows anti-influenza virus (ifv) potential as a probiotic, partly through enhancement of innate immunity by modulation of the intestinal immune system. bifidobacterium longum mm-2 (mm-2), a very safe bacterium in humans, was isolated from healthy humans and its protective effect against ifv infection in a murine model shown. in mice that were intranasally inoculated with ifv, oral administration of mm-2 for 17 consecutive day ... | 2015 | 25400245 |
| bifidobacterium longum subsp. infantis bb-02 attenuates acute murine experimental model of inflammatory bowel disease. | inflammatory bowel diseases (ibd) are chronic inflammatory conditions, characterised by remissions and relapses episodes, whose main manifestations are ulcerative colitis and crohn's disease. ulcerative colitis (uc), one of the main forms of ibd, has as standard treatment the use of corticosteroids and anti-inflammatory drugs. the use of antibiotics has been also reported, but the possible adverse effects, such as disturbance of the indigenous microbiota or resistance induction, should be taken ... | 2015 | 25391346 |
| the effect of a multi-strain probiotic on the resistance toward escherichia coli challenge in a randomized, placebo-controlled, double-blind intervention study. | several probiotic strains have been shown to enhance human resistance to infectious disease. it is speculated that these strains may impose this effect by excretion of anti-microbial components, by competing with pathogens for intestinal nutrients and/or mucosal adhesion sites or modulating the immune system. | 2015 | 25369827 |
| feed supplementation with red seaweeds, chondrus crispus and sarcodiotheca gaudichaudii, affects performance, egg quality, and gut microbiota of layer hens. | the aim of this study was to evaluate the effect of the inclusion of red seaweed supplementation to standard poultry diets on production performance, egg quality, intestinal histology, and cecal short-chain fatty acids in lohmann brown classic laying hens. a total of 160 birds were randomly assigned to 8 treatment groups. control hens were fed a basal layer diet; positive control hens were fed a diet containing 2% inulin; and 6 treatment groups were fed a diet containing one of the following; 0. ... | 2014 | 25352682 |
| probiotics for preventing ventilator-associated pneumonia. | ventilator-associated pneumonia (vap) is common in intensive care units (icus). some evidence indicates that probiotics may reduce the incidence of vap. several additional published studies have demonstrated that probiotics are safe and efficacious in preventing vap in icus. we aimed to systematically summarise the results of all available data to generate the best evidence for the prevention of vap. | 2014 | 25344083 |
| acarbose treatment affects the serum levels of inflammatory cytokines and the gut content of bifidobacteria in chinese patients with type 2 diabetes mellitus. | the effects of acarbose add-on therapy on gut microbiota and inflammatory cytokines were investigated in chinese patients with type 2 diabetes mellitus (dm). | 2015 | 25327485 |
| bifidobacterium longum subspecies infantis: champion colonizer of the infant gut. | oligosaccharides are abundant in human milk. production of these highly diverse structures requires significant energy expenditure by the mother and yet these human milk oligosaccharides offer no direct nutritive value to her infant. a primary function of human milk oligosaccharides is to shape the infant's intestinal microbiota with life-long consequences. bifidobacterium longum subspecies infantis (b. infantis) is unique among gut bacteria in its prodigious capacity to digest and consume any h ... | 2015 | 25303277 |
| selective growth-inhibitory effect of 8-hydroxyquinoline towards clostridium difficile and bifidobacterium longum subsp. longum in co-culture analysed by flow cytometry. | the major risk factor for clostridium difficile infection (cdi) is the use of antibiotics owing to the disruption of the equilibrium of the host gut microbiota. to preserve the beneficial resident probiotic bacteria during infection treatment, the use of molecules with selective antibacterial activity enhances the efficacy by selectively removing c. difficile. one of them is the plant alkaloid 8-hydroxyquinoline (8hq), which has been shown to selectively inhibit clostridia without repressing bif ... | 2014 | 25298160 |
| use of selected lactic acid bacteria in the eradication of helicobacter pylori infection. | helicobacter pylori is among the major pathogenic bacteria that cause chronic gastritis and peptic ulcer disease and is related to the development of gastric cancer. several chemicals, including antibiotics, have been used to eradicate h. pylori; however, they do not always curb the infection. ten representative type strains of lactic acid bacteria (lab) were screened for antagonism toward h. pylori via inhibition of urease activity. strains inhibiting the binding of h. pylori to human gastric c ... | 2014 | 25277407 |
| bifidobacteria exert strain-specific effects on stress-related behavior and physiology in balb/c mice. | accumulating evidence suggests that commensal bacteria consumption has the potential to have a positive impact on stress-related psychiatric disorders. however, the specific bacteria influencing behaviors related to anxiety and depression remain unclear. to this end, we compared the effects of two different bifidobacteria on anxiety and depression-like behavior; an antidepressant was also used as a comparator. | 2014 | 25251188 |
| prebiotic oligosaccharides: comparative evaluation using in vitro cultures of infants' fecal microbiomes. | the objective of this study was to systematically assess the bifidogenic effect of three commonly used prebiotic products using in vitro cultures of infant fecal samples. fresh stool samples collected from six term infants, each exclusively fed human milk (n = 3) or infant formula (n = 3), at 28 days of age were used as inocula. the following prebiotic products were added at concentrations applicable to infant formula: vivinal gos 15 (containing 28.5% galacto-oligosaccharide [gos]) at 7.2 g/lite ... | 2014 | 25239906 |
| functional role of tlyc1 encoding a hemolysin-like protein from bifidobacterium longum bbmn68 in bile tolerance. | bifidobacteria are normal inhabitants of the human gut, and members of which are generally considered to be probiotic. before exerting their beneficial properties, they must survive and persist in the physiological concentrations (0.05-2%) of bile in the gut. in this work, the functional role of tlyc1 encoding a hemolysin-like protein from bifidobacterium longum bbmn68 in bile tolerance was tested. analysis using the program tmhmm and homologous alignment indicated that tlyc1 is a nontransporter ... | 2014 | 25227940 |
| [construction and identification of the bifidobacterium expression system pgex-tsol18/b. longum of taenia solium]. | the tsol18 gene of taenia solium was synthesized and cloned into escherichia coli-bifidobacteria shuttle vector pgex-1lambdat. the recombinant plasmid pgex-tsol18 was transformed into bifidobacterium longum with electroporation. the recombinant plasmid containing tsol18 gene was identified by restriction endonuclease analysis, pcr and dna sequencing. the length of synthesized tsol18 gene was 393 bp. the results indicated that the bifidobacteria expression system pgex-tsol18/b. longum was success ... | 2014 | 25223064 |
| [correlation of the microbiota and intestinal mucosa in the pathophysiology and treatment of irritable bowel, irritable eye, and irritable mind syndrome]. | accumulating clinical evidence supports co-morbidity of irritable bowel, irritable eye and irritable mind symptoms. furthermore, perturbation of the microbiota-host symbiosis (dysbiosis) is considered a common pathogenic mechanism connecting gastrointestinal, ocular and neuropsychiatric symptoms. consequently, maintaining or restoring microbiota-host symbiosis represents a new approach to treat these symptoms or to prevent their relapses. current treatment approach assigned a primary role to liv ... | 2014 | 25194867 |
| effect of electroporation on bioconversion of isoflavones and probiotic properties of parents and subsequent passages of bifidobacterium longum. | this study aimed to evaluate the effects of electroporation on growth, bioconversion of isoflavones, and probiotic properties of parent organisms and subsequent passages of bifidobacterium longum ftdc 8643. electroporation with the strength of electric field at 7.5 kv cm(-1) for 3.5 ms was applied on b. longum ftdc 8643. the viability of b. longum ftdc 8643 increased significantly upon treatment with electroporation. such treatment also enhanced the intracellular and extracellular β-glucosidase ... | 2014 | 25119552 |
| effect of traditional leafy vegetables on the growth of lactobacilli and bifidobacteria. | traditional leafy vegetables, apart from being a staple in the diet of most of sub-saharan africa, are an essential part of traditional medicine and are used daily by traditional healers in the region to treat a wide variety of ailments. in this study, a batch culture technique was used to investigate whether 25 infusions from 22 traditional leafy vegetables stimulated the growth of lactobacillus bulgaricus, lactobacillus lactis, lactobacillus reuteri and bifidobacterium longum in pure culture. ... | 2014 | 25088723 |
| influence of tea extract supplementation on bifidobacteria during soymilk fermentation. | in this study, the influence of tea extract (te) supplementation on the viability and membrane lipid compositions of bifidobacterium was investigated. fermented soymilk-tea (smt) was produced by culturing selected bifidobacteria in soymilk supplemented with green or black te. culturability of four bacteria in the presence of various concentrations of te was examined by plate count method. bifidobacterium longum cscc 5089 (bl5089) and b. longum cscc 5022 (bl5022) were selected for further study b ... | 2014 | 25086351 |
| cloning, purification and preliminary crystallographic analysis of ara127n, a gh127 β-l-arabinofuranosidase from geobacillus stearothermophilus t6. | the l-arabinan utilization system of geobacillus stearothermophilus t6 is composed of five transcriptional units that are clustered within a 38 kb dna segment. one of the transcriptional units contains 11 genes, the last gene of which (aran) encodes a protein, ara127n, that belongs to the newly established gh127 family. ara127n shares 44% sequence identity with the recently characterized hypba1 protein from bifidobacterium longum and thus is likely to function similarly as a β-l-arabinofuranosid ... | 2014 | 25084377 |
| effect of kcl substitution on bacterial viability of escherichia coli (atcc 25922) and selected probiotics. | excessive intake of nacl has been associated with the increased risk of several diseases, particularly hypertension. strategies to reduce sodium intake include substitution of nacl with other salts, such as kcl. in this study, the effects of nacl reduction and its substitution with kcl on cell membranes of a cheese starter bacterium (lactococcus lactis ssp. lactis), probiotic bacteria (bifidobacterium longum, lactobacillus acidophilus, and lactobacillus casei), and a pathogenic bacterium (escher ... | 2014 | 25064650 |
| the influence of incubation time, sample preparation and exposure to oxygen on the quality of the maldi-tof ms spectrum of anaerobic bacteria. | with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms), bacteria can be identified quickly and reliably. this accounts especially for anaerobic bacteria. because growth rate and oxygen sensitivity differ among anaerobic bacteria, we aimed to study the influence of incubation time, exposure to oxygen and sample preparation on the quality of the spectrum using the bruker system. also, reproducibility and inter-examiner variability were determined. twenty-s ... | 2014 | 25039504 |
| discovery of lipids from b. longum subsp. infantis using whole cell maldi analysis. | bifidobacteria are dominant members of the microbial community in the intestinal tract of infants, and studies have shown that glycolipids extracted from the cell surface of these bacteria elicit beneficial immune responses. accordingly, the identification and structural characterization of glycolipids from the cell wall of bifidobacteria is the first step in correlating glycolipid structure with biological activity. using whole cell maldi as a screening tool, we herein present for the first tim ... | 2014 | 25032516 |
| survival of bifidobacterium longum and its effect on physicochemical properties and sensorial attributes of white brined cheese. | survival of the probiotic adjunct culture bifidobacterium longum and cheese starters during ripening of white brined cheese, effect of the probiotic culture on physicochemical properties and sensorial attributes of cheeses were investigated throughout 90 d of ripening. bifidobacterium longum were able to survive at higher levels (>10(7 )cfu/g cheese) than the therapeutic minimum (10(6)-10(7 )cfu/g cheese) after 90 d and did not have any negative effect on the survival of streptococcus spp. (incl ... | 2014 | 25003962 |
| stool microbiota and vaccine responses of infants. | oral vaccine efficacy is low in less-developed countries, perhaps due to intestinal dysbiosis. this study determined if stool microbiota composition predicted infant oral and parenteral vaccine responses. | 2014 | 25002669 |
| bifidobacterium longum subsp. infantis in experimental necrotizing enterocolitis: alterations in inflammation, innate immune response, and the microbiota. | probiotics decrease the risk of necrotizing enterocolitis (nec). we sought to determine the impact of bifidobacterium longum subsp. infantis (b. infantis) in the established rat model of nec. | 2014 | 25000347 |
| membrane fatty acid composition and fluidity are involved in the resistance to freezing of lactobacillus buchneri r1102 and bifidobacterium longum r0175. | determinations of membrane fatty acid composition and fluidity were used together with acidification activity and viability measurements to characterize the physiological state after freezing of lactobacillus buchneri r1102 and bifidobacterium longum r0175 cells harvested in the exponential and stationary growth phases. for both strains, lower membrane fluidity was achieved in cells harvested in the stationary growth phase. this change was linked to a lower unsaturated-to-saturated fatty acid ra ... | 2015 | 24981007 |
| microencapsulated bifidobacterium longum subsp. infantis atcc 15697 favorably modulates gut microbiota and reduces circulating endotoxins in f344 rats. | the gut microbiota is a bacterial bioreactor whose composition is an asset for human health. however, circulating gut microbiota derived endotoxins cause metabolic endotoxemia, promoting metabolic and liver diseases. this study investigates the potential of orally delivered microencapsulated bifidobacterium infantis atcc 15697 to modulate the gut microbiota and reduce endotoxemia in f344 rats. the rats were gavaged daily with saline or microencapsulated b. infantis atcc 15697. following 38 days ... | 2014 | 24967382 |
| homologous overexpression of alkyl hydroperoxide reductase subunit c (ahpc) protects bifidobacterium longum strain ncc2705 from oxidative stress. | the ability to manage reactive oxygen species (ros) effectively is crucial for the survival of gut bifidobacteria under conditions of oxidative stress. alkyl hydroperoxide reductase catalytic subunit c (ahpc) of bifidobacterium longum responds to various oxidative stresses. in this study, an ahpc-overexpressing transformant of b. longum strain ncc2705 was constructed to investigate the role and function of ahpc in oxidative stresses inflicted by treatments with hydrogen peroxide (h2o2), cumene h ... | 2014 | 24953679 |
| functional analysis of bifidobacterial promoters in bifidobacterium longum and escherichia coli using the α-galactosidase gene as a reporter. | heterologous gene expression in bifidobacteria requires weak, strong, and inducible promoters depending on the objectives of different expression studies. weak promoters in escherichia coli can also be desirable for stable heterologous gene cloning. here, we developed a reporter system using the bifidobacterium longum α-galactosidase gene and investigated the activity and inducibility of seven bifidobacterial promoters in b. longum and their activities in e. coli. these studies revealed diverse ... | 2014 | 24932968 |
| combination of heterogeneous catalase and superoxide dismutase protects bifidobacterium longum strain ncc2705 from oxidative stress. | bifidobacteria are generally sensitive to oxidative stress caused by reactive oxygen species (ros). to improve oxidative-stress tolerance, the superoxide dismutase (sod) gene from streptococcus thermophilus (stsoda) and the heme-dependent catalase (kat) gene from lactobacillus plantarum (lpkatl) were heterologously expressed in bifidobacterium longum strain ncc2705. three types of strain ncc2705 transformants were obtained: with transgenic sod expression, with transgenic kat expression, and with ... | 2014 | 24903816 |
| the relationship between bifidobacteria and allergic asthma and/or allergic dermatitis: a prospective study of 0-3 years-old children in turkey. | bifidobacteria are beneficial bacteria for humans. these bacteria are particularly effective at protecting against infectious diseases and modulating the immune response. it was shown that in newborns, the fecal distribution of the colonizing bifidobacterium species influences the prevalence of allergic diseases. this study aimed to compare the faecal bifidobacterium species of allergic children to those of healthy children to detect species level differences in faecal distribution. stool sample ... | 2014 | 24878320 |
| preparation of selenium-enriched bifidobacterium longum and its effect on tumor growth and immune function of tumor-bearing mice. | in this study, we demonstrated selenium (se) accumulation in bifidobacterium longum strain (b. longum) and evaluated the effect of se-enriched b. longum (se-b. longum) on tumor growth and immune function in tumor-bearing mice. analysis using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (hplc-icp-ms) revealed that more than 99% of se in se-b. longum was organic, the main component of which was selenomethionine (semet). in the in vivo experiments, tumor-beari ... | 2014 | 24870777 |
| proteomic analysis of the interaction of bifidobacterium longum ncc2705 with the intestine cells caco-2 and identification of plasminogen receptors. | to identify proteins with a potential role in the interaction of bifidobacterium longum with intestinal epithelial cells, we profiled the protein response of b. longum ncc2705 following interaction with caco-2 cells. thirty-one protein spots, belonging to a total of 23 proteins, which exhibited a change in abundance of at least 3-fold were identified in b. longum ncc2705 following co-culture with caco-2 cells, and were subsequently identified. changes in expression were confirmed at the transcri ... | 2014 | 24840471 |
| bifidobacterium longum subsp. longum exo-β-1,3-galactanase, an enzyme for the degradation of type ii arabinogalactan. | type ii arabinogalactan (ag-ii) is a suitable carbohydrate source for bifidobacterium longum subsp. longum, but the degradative enzymes have never been characterized. in this study, we characterized an exo-β-1,3-galactanase, bllj_1840, belonging to glycoside hydrolase family 43 from b. longum subsp. longum jcm1217. the recombinant bllj_1840 expressed in escherichia coli hydrolyzed β-1,3-linked galactooligosaccharides but not β-1,4- and β-1,6-linked galactooligosaccharides. the enzyme also hydrol ... | 2014 | 24837371 |
| lactobacillus rhamnosus gg and bifidobacterium longum attenuate lung injury and inflammatory response in experimental sepsis. | probiotic use to prevent nosocomial gastrointestinal and potentially respiratory tract infections in critical care has shown great promise in recent clinical trials of adult and pediatric patients. despite well-documented benefits of probiotic use in intestinal disorders, the potential for probiotic treatment to reduce lung injury following infection and shock has not been well explored. | 2014 | 24830455 |
| crystallization and preliminary x-ray diffraction analysis of a novel β-l-arabinofuranosidase (hypba1) from bifidobacterium longum. | the β-l-arabinofuranosidase (hypba1) from bifidobacterium longum jcm 1217 hydrolyzes the β-1,2-linked arabinofuranose disaccharide to release l-arabinoses. hypba1 was classified into glycoside hydrolase family 127 (gh127) by the cazy website (http://www.cazy.org/). the enzyme was expressed in escherichia coli and the purified recombinant protein was crystallized. crystals belonging to the primitive hexagonal space group p3x21, with unit-cell parameters a = b = 75.9, c = 254.0å, were obtained by ... | 2014 | 24817727 |
| insights into the binding specificity and catalytic mechanism of n-acetylhexosamine 1-phosphate kinases through multiple reaction complexes. | utilization of n-acetylhexosamine in bifidobacteria requires the specific lacto-n-biose/galacto-n-biose pathway, a pathway differing from the leloir pathway while establishing symbiosis between humans and bifidobacteria. the gene lnpb in the pathway encodes a novel hexosamine kinase nahk, which catalyzes the formation of n-acetylhexosamine 1-phosphate (glcnac-1p/galnac-1p). in this report, seven three-dimensional structures of nahk in complex with glcnac, galnac, glcnac-1p, glcnac/amppnp and glc ... | 2014 | 24816108 |
| antiradical and tea polyphenol-stabilizing ability of functional fermented soymilk-tea beverage. | this study examined the potential of two-step fermentation to preserve tps in functional soy-tea beverage. fermented soymilk-tea (fst) was produced by culturing streptococcus thermophilus, lactobacillus delbrueckii ssp. bulgaricus and bifidobacterium longum in soymilk supplemented with tea extract (te). total phenolic content (tpc) and anti-radical activities were determined for fsts and fermented soymilk (fs). a hplc method was employed to quantify nine major tea phenolics in fst products. tpc ... | 2014 | 24731340 |
| an in vitro study of the effect of probiotics, prebiotics and synbiotics on the elderly faecal microbiota. | the use of dietary intervention in the elderly in order to beneficially modulate their gut microbiota has not been extensively studied. the influence of two probiotics (bifidobacterium longum and lactobacillus fermentum) and two prebiotics [isomaltooligosaccharides (imo) and short-chain fructooligosaccharides (fos)], individually and in synbiotic combinations (b. longum with imo, l. fermentum with fos) on the gut microbiota of elderly individuals was investigated using faecal batch cultures and ... | 2014 | 24685554 |
| crystal structure of glycoside hydrolase family 127 β-l-arabinofuranosidase from bifidobacterium longum. | enzymes acting on β-linked arabinofuranosides have been unknown until recently, in spite of wide distribution of β-l-arabinofuranosyl oligosaccharides in plant cells. recently, a β-l-arabinofuranosidase from the glycoside hydrolase family 127 (hypba1) was discovered in the newly characterized degradation system of hydroxyproline-linked β-l-arabinooligosaccharides in the bacterium bifidobacterium longum. here, we report the crystal structure of hypba1 in the ligand-free and β-l-arabinofuranose co ... | 2014 | 24680821 |
| entrapment of probiotics in water extractable arabinoxylan gels: rheological and microstructural characterization. | due to their porous structure, aqueous environment and dietary fiber nature arabinoxylan (ax) gels could have potential applications for colon-specific therapeutic molecule delivery. in addition, prebiotic and health related effects of ax have been previously demonstrated. it has been also reported that cross-linked ax can be degraded by bacteria from the intestinal microbiota. however, ax gels have not been abundantly studied as carrier systems and there is no information available concerning t ... | 2014 | 24662078 |
| oral administration of genetically modified bifidobacterium displaying hcv-ns3 multi-epitope fusion protein could induce an hcv-ns3-specific systemic immune response in mice. | more than 170 million people worldwide are chronic hcv (hepatitis c virus) carriers, and about 30% of them will develop progressive liver disease, such as cirrhosis and hepatocellular carcinoma. a combination of pegylated interferon-α with ribavirin, the standard treatment for hcv infection, has been effective in fewer than 50% of patients infected with hcv genotype 1. a strong t cell response against the nonstructural protein 3 (ns3) is important for recovery from acute hcv infection, and an ea ... | 2014 | 24657718 |
| peritonitis caused by bifidobacterium longum: case report and literature review. | bifidobacterium spp. rarely causes human infections. we report a case of a 42-year-old man with a history of pancolonic diverticulosis, who suffered a purulent peritonitis caused by bifidobacterium longum secondary to intestinal perforation. clinical outcome was good after urgent surgery and antibiotic treatment with imipenem and amoxicillin/clavulanic acid. our case shows that bifidobacterium spp. should be considered as a cause of peritonitis, especially in patients with risk of intestinal per ... | 2014 | 24657157 |
| production of α- and β-galactosidases from bifidobacterium longum subsp. longum rd47. | approximately 50% of people in the world experience abdominal flatulence after the intake of foods containing galactosides such as lactose or soybean oligosaccharides. the galactoside hydrolyzing enzymes of α- and β-galactosidases have been shown to reduce the levels of galactosides in both the food matrix and the human gastrointestinal tract. this study aimed to optimize the production of α- and β-galactosidases of bifidobacterium longum subsp. longum rd47 with a basal medium containing whey an ... | 2014 | 24608564 |
| bifidobacteria exhibit luxs-dependent autoinducer 2 activity and biofilm formation. | autoinducer-2 (ai-2) molecules are one class of signalling molecules involved in gene regulation dependent on population density in a mechanism commonly referred to as quorum sensing (qs). ai-2 is produced by the methylthioadenosine/s-adenosyl-homocysteine nucleosidase luxs. in the present study, we characterise the function of bifidobacterial luxs proteins to address the question whether these economically important bacteria are able to perform qs communication. all publically available genome ... | 2014 | 24505453 |
| characterization of a novel β-l-arabinofuranosidase in bifidobacterium longum: functional elucidation of a duf1680 protein family member. | pfam duf1680 (pf07944) is an uncharacterized protein family conserved in many species of bacteria, actinomycetes, fungi, and plants. previously, we cloned and characterized the hypba2 gene as a β-l-arabinobiosidase in bifidobacterium longum jcm 1217. in this study, we cloned a duf1680 family member, the hypba1 gene, which constitutes a gene cluster with hypba2. hypba1 is a novel β-l-arabinofuranosidase that liberates l-arabinose from the l-arabinofuranose (araf)-β1,2-araf disaccharide. hypba1 al ... | 2014 | 24385433 |
| probiotic gut effect prevents the chronic psychological stress-induced brain activity abnormality in mice. | a probiotic formulation (lactobacillus helveticus r0052 and bifidobacterium longum r0175 combination, probio'stick(®) ) displays anxiolytic-like activity and reduces apoptosis in the lymbic system in animal models of depression. based on the hypothesis that modulation of gut microbiota by this probiotic formulation has beneficial effects on brain activity in stress conditions, we report a set of probiotic-evoked physiological, cellular, and molecular events in the brain of probio'stick(®) pretre ... | 2014 | 24372793 |
| goat milk yoghurt by using lacto-b culture modulates the production of tumor necrosis factor-alpha and interleukin-10 in malnourished rats. | total spleen lymphocytes, lymphocyte proliferation, tumor necrosis factor-α (tnf-α), and interleukin-10 (il-10) in spleen lymphocyte culture were studied in malnourished wistar rats fed with goat milk yoghurt. malnourished rats were created by using standard feed restriction as much as 50% of normal rats for 21 d. goat milk yoghurt containing three types of microorganism e.g., lactobacillus acidophilus, sterptococcus thermophilus and bifidobacterium longum derived from lacto-b culture in powder ... | 2014 | 26760750 |
| high-quality draft genome sequence of bifidobacterium longum e18, isolated from a healthy adult. | bifidobacteria are important gastrointestinal commensals of a number of animals, including humans, and various beneficial effects on host health have been attributed to them. here, we announce the noncontiguous finished genome sequence of bifidobacterium longum e18, isolated from a healthy adult, which reveals traits involved in its interaction with the host. | 2013 | 24356845 |
| distinct substrate specificities of three glycoside hydrolase family 42 β-galactosidases from bifidobacterium longum subsp. infantis atcc 15697. | glycoside hydrolase family 42 (gh42) includes β-galactosidases catalyzing the release of galactose (gal) from the non-reducing end of different β-d-galactosides. health-promoting probiotic bifidobacteria, which are important members of the human gastrointestinal tract microbiota, produce gh42 enzymes enabling utilization of β-galactosides exerting prebiotic effects. however, insight into the specificity of individual gh42 enzymes with respect to substrate monosaccharide composition, glycosidic l ... | 2014 | 24270321 |
| bifidobacterium longum alleviates dextran sulfate sodium-induced colitis by suppressing il-17a response: involvement of intestinal epithelial costimulatory molecules. | although some bacterial strains show potential to prevent colitis, their mechanisms are not fully understood. here, we investigated the anti-colitic mechanisms of bifidobacterium longum subsp. infantis jcm 1222(t), focusing on the relationship between interleukin (il)-17a secreting cd4(+) t cells and intestinal epithelial costimulatory molecules in mice. oral administration of jcm 1222(t) to mice alleviated dextran sulfate sodium (dss)-induced acute colitis. the expression of type 1 helper t (th ... | 2013 | 24255712 |
| a randomized double-blind controlled trial: impact of probiotics on diarrhea in patients treated with pelvic radiation. | radical radiation therapy is commonly used for treatment of pelvic cancer. up to 80% of patients receiving radiotherapy will develop acute radiation induced diarrhea. the primary aim of this randomized double blind controlled trial is to evaluate the effect of the probiotic bifilact(®) on moderate and severe treatment-induced diarrhea during pelvic radiation. | 2014 | 24200199 |
| characterization of a novel β-l-arabinofuranosidase in bifidobacterium longum. functional elucidation of a duf1680 family member. | | 2013 | 24143008 |
| enrichment of bifidobacterium longum subsp. infantis atcc 15697 within the human gut microbiota using alginate-poly-l-lysine-alginate microencapsulation oral delivery system: an in vitro analysis using a computer-controlled dynamic human gastrointestinal model. | this study evaluates alginate-poly-l-lysine-alginate bifidobacterium longum subsp. infantis atcc 15697-loaded microcapsules to enrich the human gut microbiota. the cell survival of alginate-poly-l-lysine-alginate microencapsulated b. infantis atcc 15697 in gastric acid, bile, and through human gastrointestinal transit was investigated, as well as the formulation's effect on the gut microbiota. results show that microencapsulation increases b. infantis atcc 15697 cell survival at ph1.0 (33.54 ± 2 ... | 2014 | 24124888 |
| cell-free preparations of lactobacillus acidophilus strain la-5 and bifidobacterium longum strain ncc2705 affect virulence gene expression in campylobacter jejuni. | campylobacter spp. are among the most commonly reported bacterial causes of acute diarrheal disease in humans worldwide. potential virulence factors include motility, chemotaxis, colonization ability, adhesion to intestinal cells, invasion and epithelial translocation, intracellular survival, and formation of toxins. probiotic lactobacillus and bifidobacterium strains are known to have an inhibitory effect against the growth of various foodborne pathogens. the objective of this study was to inve ... | 2013 | 24112574 |
| probiotic modulation of dendritic cell function is influenced by ageing. | dendritic cells (dcs) are critical for the generation of t-cell responses. dc function may be modulated by probiotics, which confer health benefits in immunocompromised individuals, such as the elderly. this study investigated the effects of four probiotics, bifidobacterium longum bv. infantis ccug 52486, b. longum sp 07/3, lactobacillus rhamnosus gg (l.gg) and l. casei shirota (lcs), on dc function in an allogeneic mixed leucocyte reaction (mlr) model, using dcs and t-cells from young and older ... | 2014 | 24094416 |
| neonatal colonization of germ-free mice with bifidobacterium longum prevents allergic sensitization to major birch pollen allergen bet v 1. | the main goal in reversing the allergy epidemic is the development of effective prophylactic strategies. we investigated the prophylactic effect of neonatal mother-to-offspring mono-colonization with bifidobacterium longum ssp. longum ccm 7952 on subsequent allergic sensitization. adult male and female germ-free (gf) mice were mono-colonized with b. longum, mated and their offspring, as well as age-matched gf controls, were sensitized with the major birch pollen allergen bet v 1. furthermore, si ... | 2013 | 24055352 |
| preliminary selection for potential probiotic bifidobacterium isolated from subjects of different chinese ethnic groups and evaluation of their fermentation and storage characteristics in bovine milk. | a total of 29 strains of bifidobacterium were isolated from 18 samples of human feces in different ethnic minority regions of china. all isolates were identified as bifidobacterium longum (9 strains) and bifidobacterium pseudocatenulatum (20 strains) based on 16s rrna gene sequencing and phylogenetic analysis. these strains were preliminarily tested for their suitability to become probiotics by assessing their ability to survive adequately at low ph conditions and their tolerance of different co ... | 2013 | 24054292 |
| genome sequence of the bacterium bifidobacterium longum strain cmcc p0001, a probiotic strain used for treating gastrointestinal disease. | bifidobacterium longum subsp. longum cmcc p0001, a standard probiotic strain in china, has been widely used in clinical medicine for more than 20 years. here we report the genome features of b. longum strain cmcc p0001. | 2013 | 24029762 |
| supercritical co2 interpolymer complex encapsulation improves heat stability of probiotic bifidobacteria. | the probiotic industry faces the challenge of retention of probiotic culture viability as numbers of these cells within their products inevitably decrease over time. in order to retain probiotic viability levels above the therapeutic minimum over the duration of the product's shelf life, various methods have been employed, among which encapsulation has received much interest. in line with exploitation of encapsulation for protection of probiotics against adverse conditions, we have previously en ... | 2014 | 23990069 |
| synbiotic consumption changes the metabolism and composition of the gut microbiota in older people and modifies inflammatory processes: a randomised, double-blind, placebo-controlled crossover study. | ageing can result in major changes in the composition and metabolic activities of bacterial populations in the large gut and an impaired immune system. | 2013 | 23957631 |
| efficacy and safety of probiotics in the treatment of candida-associated stomatitis. | this study aimed at evaluating the short-term efficacy and safety of probiotics as an aid in the treatment of candida-associated stomatitis in a randomised controlled trial. a total of 65 patients were randomly assigned to receive oral local antifungal agents alone (gargle 2% sodium bicarbonate solution for 30 s, wait 10 min and then apply 2% nystatin paste) or these agents plus local probiotics (the mixture of bifidobacterium longum, lactobacillus bulgaricus and streptococcus thermophilus) thre ... | 2014 | 23952962 |
| fecal microbial metabolism of polyphenols and its effects on human gut microbiota. | we investigated the biotransformation of four common dietary polyphenols, rutin, quercetin, chlorogenic acid and caffeic acid, in an in vitro mixed culture model of human intestinal microbiota, to determine effects on human gut bacteria. all four compounds were biotransformed rapidly, disappearing from the medium within 0.5 h and later replaced by known phenolic acid breakdown products, at concentrations up to hundreds of micromolar, much higher than in no-polyphenol control experiments. quantit ... | 2013 | 23916722 |
| impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation. | prebiotics are considered to have potential to reduce disturbances in the gut microbiota induced by antibiotics. results in literature are, however, not consistent. the current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (gos) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses. the changes in relative abundance of bacteria upon antibiotic treatment and the gro ... | 2014 | 23909489 |
| [outcomes of clostridium difficile enterocolitis after administration of antibiotics along with probiotic supplement]. | clostridium difficile enterocolitis is a potentially fatal disease showing increasing incidence in hospital environment. therapeutic approach in the management of clostridium difficile enterocolitis is highly complex, particularly because of its tendency to relapse and reinfection. the study was aimed at investigating the factors influencing the development of clostridium difficile enterocolitis and outcomes of enterocolitis after administration of standard antimicrobial therapy combined with pr ... | 2013 | 23888728 |
| dietary supplementation of bifidobacterium longum strain ah1206 increases its cecal abundance and elevates intestinal interleukin-10 expression in the neonatal piglet. | intestinal microbiota of infants differ in response to gestational age, delivery mode and feeding regimen. dietary supplementation of probiotic bacteria is one method of promoting healthy populations. we examined the impact of a novel probiotic strain of bifidobacterium longum (ah1206) on the health, growth and development of neonatal pigs as a model for infants. day-old pigs were fed milk-based formula containing ah1206 at 0, 10⁹, or 10¹¹ cfu/d for 18 d (n=10/treatment). differences were not de ... | 2013 | 23872134 |
| casein hydrolysis by bifidobacterium longum kacc91563 and antioxidant activities of peptides derived therefrom. | milk protein is a well-known precursor protein for the generation of bioactive peptides using lactic acid bacteria. this study investigated the antioxidant activity of bovine casein hydrolysate after fermentation with bifidobacterium longum kacc91563 using the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (abts) assay and total phenolic content (tpc). the antioxidant activities of the 24-h and 48-h hydrolysates were higher than that of the 4-h hydrolysate (2,045.5 and 1,629.3 μm gallic ... | 2013 | 23871374 |
| probiotics improve survival of septic rats by suppressing conditioned pathogens in ascites. | to investigate the benefits of probiotics treatment in septic rats. | 2013 | 23840152 |