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an outbreak of fusarium solani endophthalmitis after cataract surgery in an eye training and research hospital in istanbul.to report an outbreak of fusarium solani endophthalmitis after uneventful cataract surgeries performed on the same day in the same operating room. nine patients underwent phacoemulsification at 4th clinic of beyoglu eye training and research hospital in istanbul. cefuroxime axetyl was injected intracamerally from the same vial to all patients at the end of surgery. all patients developed acute postoperative endophthalmitis. presentation, cultural studies, treatment, clinical responses and risk f ...201121627695
synthesis, characterization and biological properties of thienyl derived triazole schiff bases and their oxovanadium(iv) complexes.a new series of biologically active thienyl derived triazole schiff bases and their oxovanadium(iv) complexes have been synthesized and characterized on the basis of physical (m.p., magnetic susceptibility and conductivity), spectral (ir, (1)h and (13)c nmr, electronic and mass spectrometry) and microanalytical data. all the schiff base ligands and their oxovanadium(iv) complexes have been subjected to in vitro antibacterial activity against four gram-negative (escherichia coli, shigella flexner ...201121635212
[antagonism of trichoderma spp. to fungi caused root rot of sophora tonkinensis].to study the antagonism of trichoderma spp. to fungi s9(fusarium solani)which caused root rot of sophora tonkinensis and discuss the further develop prospects of microbial biological control in soil-borne diseases on chinese herbal medicines.201121809533
osmotin from calotropis procera latex: new insights into structure and antifungal properties.this study aimed at investigating the structural properties and mechanisms of the antifungal action of cposm, a purified osmotin from calotropis procera latex. fluorescence and cd assays revealed that the cposm structure is highly stable, regardless of ph levels. accordingly, cposm inhibited the spore germination of fusarium solani in all ph ranges tested. the content of the secondary structure of cposm was estimated as follows: a-helix (20%), ß-sheet (33%), turned (19%) and unordered (28%), rms ...201121798235
efficacy of oral e1210, a new broad-spectrum antifungal with a novel mechanism of action, in murine models of candidiasis, aspergillosis, and fusariosis.e1210 is a first-in-class, broad-spectrum antifungal with a novel mechanism of action - inhibition of fungal glycosylphosphatidylinositol biosynthesis. in this study, the efficacies of e1210 and reference antifungals were evaluated in murine models of oropharyngeal and disseminated candidiasis, pulmonary aspergillosis, and disseminated fusariosis. oral e1210 demonstrated dose dependent efficacy in these infections caused by candida species, aspergillus spp. and fusarium solani. in the treatment ...201121788462
fusarium soloni mycetoma.a young apparently healthy, non-diabetic, hiv non-reactive woman presented with a mycetoma-like lesion on right buttock. discharge was scanty, and mycotic grains were not seen. biopsy of sinus track was obtained for microscopy and culture. microscopic examination revealed plenty of fungal hyphae in direct microscopic examination of grounded tissues in saline; koh, gram's, and h and e-stained smears. all the three inoculated slants of sabouraud's media yielded heavy growth of fusarium solani. pre ...201121772597
amphotericin b and voriconazole susceptibility profiles for the fusarium solani species complex: comparison between the e-test and clsi m38-a2 microdilution methodology. 201121744037
[mixed infection caused by meloidogyne and pathogenic fungi on siraitia grosvenorii].to study the mixed infection caused by meloidogyne and pathogenic fungi on siraitia grosvenorii,the result can provide basis for controltion.201121818963
epidemiological and clinico-mycological profile of fungal wound infection from largest burn centre in asia.the current study was conducted to know the incidence, predisposing factors, spectrum, clinical profile and antifungal susceptibility (afs) of fungal wound infection (fwi) in burn patients. of a total of 71 patients, 20 (28.2%) emerged with the diagnosis of fwi. fungal pathogens in this study were candida tropicalis (14%), candida parapsilosis (5.6%), aspergillus niger (2.8%) and one each of candida albicans (1.4%), candida glabrata (1.4%), syncephalestrum (1.4%) and fusarium solani (1.4%). all ...201121740469
novel microbial transformation of resibufogenin by fusarium solani.in this paper, microbial transformation of resibufogenin by fusarium solani as 3.1829 was investigated, and five transformed products were isolated and identified as 3-ketone-resibufogenin (2), 3-one-cyclic 3-(1,2-dimethyl-1,2-ethanediylacetal)-resibufogenin (3), 3-dimethoxyl-resibufogenin (4), 3-epi-resibufogenin (5), and 3-epi-15+¦-hydroxy-7+¦h-bufalin (6), respectively. among them, 3, 4, and 6 are new compounds, and the rare double oxidization of c-3 was reported. in addition, the cytotoxicit ...201121830888
expression of innate and adaptive immune mediators in human corneal tissue infected with aspergillus or fusarium.background.ôçâfilamentous fungi of the genera aspergillus and fusarium are major causes of corneal ulcers in the united states and in the developing world and result in significant visual impairment and blindness. methods.ôçârna was extracted from 110 patients with corneal ulcers in southern india within 1 week of infection with either fusarium solani or aspergillus flavus, and gene expression was determined by quantitative polymerase chain reaction. posttransplant corneas from later stage disea ...201121828275
kinetics and mechanism of the cutinase-catalyzed transesterification of oils in aot reversed micellar system.the kinetics of the enzymatic transesterification between a mixture of triglycerides (oils) and methanol for biodiesel production in a bis(2-ethylhexyl) sodium sulfosuccinate (aot)/isooctane reversed micellar system, using recombinant cutinase from fusarium solani pisi as a catalyst, was investigated. in order to describe the results that were obtained, a mechanistic scheme was proposed, based on the literature and on the experimental data. this scheme includes the following reaction steps: the ...201121739170
the synthetic strigolactone gr24 influences the growth pattern of phytopathogenic fungi.strigolactones that are released by plant roots to the rhizosphere are involved in both plant symbiosis with arbuscular mycorrhizal fungi and in plant infection by root parasitic plants. in this paper, we describe the response of various phytopathogenic fungi to the synthetic strigolactone gr24. when gr24 was embedded in the growth medium, it inhibited the growth of the root pathogens fusarium oxysporum f. sp. melonis, fusarium solani f. sp. mango, sclerotinia sclerotiorum and macrophomina phase ...201121688170
new species from the fusarium solani species complex derived from perithecia and soil in the old world tropics.abstract: a large collection of strains belonging to the fusarium solani species complex (fssc) was isolated from soil and perithecia in primary forests in sri lanka (from fallen tree bark) and tropical australia (queensland, from fallen tree fruits and nuts). portions of the translation elongation factor 1-alpha (tef1) gene, the nuclear large subunit (nlsu), and internal transcribed spacer regions (its) of the nuclear ribosomal rna genes were sequenced in 52 isolates from soil and perithecia. t ...201121700636
mycotic corneal ulcers in upper assam.purpose : to study the association of various risk factors and epidemiological variables of mycotic keratitis treated at a tertiary referral hospital of upper assam. materials and methods: in this hospital-based prospective study a total of 310 consecutive corneal ulcer cases attending the ophthalmology outpatient department of assam medical college were enrolled between april 2007 and march 2009. after clinical and slit-lamp biomicroscopic examination in all suspected cases, smears and culture ...201121836342
fusarium solani: an emerging fungus in chronic diabetic ulcer.fusarium species, a mold which causes disease mainly in plants has emerged as pathogen in immunocompromised patients. fusarium is known to cause keratitis, onychomycosis, and endophthalmitis. fusarium solani, is the most common isolate from clinical specimen. here is a case, a 65-year-old male with type ii diabetes mellitus since 10 years presented with a large ulcer on the left leg since 8 months following trauma. the fungal culture of the escar of the ulcer isolated a mold, fusarium solani. th ...201021814405
antibacterial effects of enniatins j(1) and j(3) on pathogenic and lactic acid bacteria.enniatins (ens) are n-methylated cyclohexadepsipeptides, secondary metabolites produced by various species of the genus fusarium. they are known to act as antifungal, antiyeast and antibacterial and to possess antiinsecticidal and phytotoxic properties. in this study we evaluated for the first time the antibiotic effect of pure fractions of en j(1) and j(3) on several pathogenic strains and lactic acid bacteria. the ens j(1) and j(3) were purified from the fermentation extract of fusarium solani ...201121742008
in vitro antifungal activity of e1210, a novel antifungal, against clinically important yeasts and moulds.e1210 is a new antifungal compound with a novel mechanism of action and broad-spectrum of antifungal activity. we investigated the in vitro antifungal activity of e1210 compared to that of fluconazole, itraconazole, voriconazole, amphotericin b, and micafungin against clinical fungal isolates. e1210 showed potent activity against most candida spp. (mic(90) of ôëñ0.008 to 0.06 ++g/ml) except for candida krusei (mics of 2 to >32 ++g/ml). e1210 showed equally potent activity against fluconazole-res ...201121825291
identification and comparison of cutinases for synthetic polyester degradation.cutinases have been exploited for a broad range of reactions, from hydrolysis of soluble and insoluble esters to polymer synthesis. to further expand the biotechnological applications of cutinases for synthetic polyester degradation, we perform a comparative activity and stability analysis of five cutinases from alternaria brassicicola (abc), aspergillus fumigatus (afc), aspergillus oryzae (aoc), humicola insolens (hic), and the well-characterized fusarium solani (fsc). of the cutinases, hic dem ...201121713515
extracellular xylanase production by fusarium species in solid state fermentation.fusarium sp. has been shown to be a promising organism for enhanced production of xylanases. in the present study, xylanase production by 21 fusarium sp. isolates (8 fusarium culmorum, 4 fusarium solani, 6 fusarium verticillioides and 3 fusarium equiseti) was evaluated under solid state fermentation (ssf). the fungal isolate fusarium solani syrn7 was the best xylanase producer among the tested isolates. the effects of some agriculture wastes (like wheat straw, wheat bran, beet pulp and cotton se ...201122184927
Alpha2-macroglobulin from an Atlantic shrimp: biochemical characterization, sub-cellular localization and gene expression upon fungal challenge.In this study, we report on the isolation and characterization of an alpha2-macroglobulin (a2M) from the plasma of the pink shrimp Farfantepenaeus paulensis, its sub-cellular localization and transcriptional changes after infection by fungi. The molecular mass of the a2M was estimated at 389 kDa by gel filtration and 197 kDa by SDS-PAGE, under reducing conditions, suggesting that a2M from F. paulensis consists of two identical sub-units, covalently linked by disulphide bonds. The N-terminal amin ...201121888978
[Identification of soil-borne fungi using Fourier transform infrared spectroscopy].Fourier transform infrared (FTIR) attenuated total reflectance (ATR) spectroscopy was used in combination with multivariate statistic analysis for identification of soil-borne fungi that causes severe economic damage to agriculture: Fusarium monili forme, Fusarium semitectum, Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Sclerotinia sclerotiorum, Pythium aphanidermatum and Phytophthora capsici. The original FTIR spectra were normalized, and the second derivatives were calculated, from ...201122007392
the in-vitro evaluation of antibacterial, antifungal and cytotoxic properties of marrubium vulgare l. essential oil grown in tunisia.in order to validate its antiseptic and anticancer properties with respect to traditional uses, we have screened for the first time the antimicrobial activity of aerial parts of m. vulgare l. essential oil against different pathogenic microorganisms and the cytotoxic activity against hela cell lines.201121936887
fungal endophthalmitis.fungal endophthalmitis (fe) is infrequent but results in poor visual outcomes. it can be exogenous or endogenous depending upon the mode of infection. the common causes for endogenous fe, post-traumatic fe and fe secondary to keratitis are candida albicans, aspergillus niger and fusarium solani, respectively. clinical features depend on the virulence of the organism and the mode of infection. broad-spectrum systemic antifungal therapy with or without intravitreal antifungal drugs is recommended. ...201122114969
performance of innovative pu-foam and natural fiber-based composites for the biofiltration of a mixture of volatile organic compounds by a fungal biofilm.the performance of perlite and two innovative carriers that consist of polyurethane (pu) chemically modified with starch; and polypropylene reinforced with agave fibers was evaluated in the biofiltration of a mixture of vocs composed of hexane, toluene and methyl-ethyl-ketone. at a total organic loading rate of 145gcm(-3)h(-1) the elimination capacities (ecs) obtained were 145, 24 and 96gcm(-3)h(-1) for the biofilters packed with the pu, the reinforced polypropylene, and perlite, respectively. s ...201122178276
successful treatment of disseminated fusariosis with voriconazole in an acute lymphoblastic leukaemia patient.fusarium species are actually the second most common pathogenic mould in immunocompromised patients, and it is difficult to treat such fusarial infections with current antifungal agents. we report the case of a 53-year-old woman with philadelphia-positive acute lymphoblastic leukaemia. during induction chemotherapy with febrile neutropenia, she developed a disseminated fusariosis, with persistent fever refractory to antibacterial agents and caspofungin (as empirical therapy), painful skin lesion ...201122126523
a novel antifungal protein from seeds of sesbania virgata (cav.) pers. (leguminosae-faboideae).a novel antifungal protein with a molecular mass around 50 kda was purified from seeds of sesbania virgata (cav.) pers. using ammonium sulfate fractionation followed by gel filtration on a sephadex g-75 superfine (sigma) column and reverse-phase high performance liquid chromatography on a c8 column. the protein, designated fp1-a, with a novel n-terminal sequence amvhspgg(s)fs(p), showed growth inhibitory activity of filamentous fungi aspergillus niger, cladosporium cladosporioides, colletotrichu ...201121881792
evaluation of luminex xtag fungal analyte-specific reagents for rapid identification of clinically relevant fungi.invasive fungal infections (ifi) remain a serious threat to immunocompromised hosts. current diagnostic methods, including fungal culture and antigen detection, are slow and often lack specificity. rapid diagnostic tools with increased sensitivity and specificity could improve the care of patients with ifi. recently, luminex molecular diagnostics (toronto, canada) developed 23 analyte-specific reagents (asrs) for the detection of the most common clinically relevant fungi. this study's objective ...201121880976
four plant defensins from an indigenous south african brassicaceae species display divergent activities against two test pathogens despite high sequence similarity in the encoding genes.abstract:201122032337
detection of invasive infection caused by fusarium solani and non-fusarium solani species using a duplex quantitative pcr-based assay in a murine model of fusariosis.a duplex real time pcr (rt-pcr) assay for detecting dna of members of the genus fusarium has been developed and validated by using two mouse models of invasive infection. the duplex rt-pcr technique employed two specific molecular beacon probes targeting a highly conserved region of the fungal rdna gene. this technique showed a detection limit of 10 fg dna per μl of sample and a specificity of 100%. the sensitivity in a total of 48 samples from a murine model of fusarium solani infection was 93. ...201121905946
Supercritical carbon dioxide biocatalysis as a novel and green methodology for the enzymatic acylation of fibrous cellulose in one step.Aliphatic esters of cellulose have recently raised the interest on the field of biopolymers. The objective of this work is to develop a methodology for the enzymatic acylation of cellulose with long chain fatty groups in one step. Therefore we designed a system at which fibrous cellulose was enzymatically acylated with vinyl laurate in supercritical carbon dioxide (scCO(2)) and as a result cellulose laurate was formed. The biocatalysts used for this reaction were immobilized lipase Candida antar ...201122014705
Synthesis, crystal structure, spectroscopic, magnetic, theoretical, and microbiological studies of a nickel(II) complex of L-tyrosine and imidazole, [Ni(Im)2(L-tyr)2]·4H2O.The [Ni(Im)(2)(L-tyr)(2)]·4H(2)O (1) complex was obtained in crystalline form as a product of interaction of L-tyrosine sodium salt, imidazole, and NiSO(4). The X-ray structure was determined, and the spectral (IR, FIR, NIR-vis-UV, HF EPR) and magnetic properties were studied. The Ni(2+) ion is hexacoordinated by the N and O atoms from two L-tyrosine molecules and by two N atoms of imidazole, resulting in a slightly distorted octahedral [NiN(2)N(2)'O(2)] geometry with a tetragonality parameter T ...201122010795
Resistant Fusarium Keratitis Progressing to Endophthalmitis.OBJECTIVE:: To report a case of multidrug-resistant Fusarium sp keratitis that progressed to endophthalmitis and that eventually required enucleation. METHODS:: Case report and literature review. Isolate identification and susceptibility testing were performed by the Fungus Testing Laboratory at San Antonio, TX. RESULTS:: A 52-year-old soft contact lens wearer had a corneal abrasion and developed a corneal infiltrate. Examination of corneal scrapings revealed filamentous hyphae with septation an ...201121993589
stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens.solanum tuberosum plants were transformed with three genetic constructions expressing the nicotiana tabacum ap24 osmotine, phyllomedusa sauvagii dermaseptin and gallus gallus lysozyme, and with a double-transgene construction expressing the ap24 and lysozyme sequences. re-transformation of dermaseptin-transformed plants with the ap24/lysozyme construction allowed selection of plants simultaneously expressing the three transgenes. potato lines expressing individual transgenes or double- and tripl ...201122115953
Treatment of Fungal Keratitis From Fusarium Infection by Corneal Cross-Linking.PURPOSE:: To evaluate the efficacy of corneal cross-linking (CXL) (riboflavin-UV-A) as a simple therapy in Fusarium keratitis. METHODS:: Twenty-four rabbits were systemically anesthetized, and the stromata of their right corneas were inoculated with Fusarium solani [10 colony-forming units (CFU) per milliliter]. Rabbits were divided into 2 groups: one was treated with CXL 72 hours after infection and the other did not receive any treatment (control). All eyes in both the groups were examined bef ...201122081155
A New Method for Evaluation of Compatibility of Contact Lenses and Lens Cases With Contact Lens Disinfecting Solutions.PURPOSE:: The purpose of this article is to describe new methodology, antimicrobial efficacy endpoint methodology to determine compatibility of contact lens solutions, lens cases and hydrogel lenses for disinfection (AEEMC), to evaluate the effect of a contact lens and a lens case on disinfection efficacy, and to present the ring test used to justify the use of the method in multiple laboratories. MATERIALS AND METHODS:: A prototype solution containing chlorhexidine as the disinfecting agent and ...201222178791
comparative analysis of fusarium mitochondrial genomes reveals a highly variable region that encodes an exceptionally large open reading frame.the mitochondrial (mt) genomes of fusarium verticillioides, fusarium solani and fusarium graminearum were annotated and found to be 53.7, 63.0 and 95.7kb in length, respectively. the genomes encode all genes typically associated with mtdnas of filamentous fungi yet are considerably larger than the mt genome of f. oxysporum. size differences are largely due to the number of group i introns. surprisingly, the genomes contain a highly variable region of 7-9kb that encodes an exceptionally large, un ...201122178648
deep granulomatous dermatitis of the fin caused by fusarium solani in a false killer whale (pseudorca crassidens).a 10-year-old female false killer whale (pseudorca crassidens) developed skin lesions in the left breast fin. histopathologically, the lesions consisted of multiple granulomas spread diffusely into the deep dermis and bone; characteristically, each granuloma has septate, branching fungal hyphae and chlamydospores surrounded by eosinophilic splendore-hoeppli materials. macrophages, epithelioid cells and multinucleated giant cells in the granulomas reacted mainly to anti-sra-e5 antibody against hu ...201122214860
endophytic fungal flora from roots and fruits of an indian neem plant azadirachta indica a. juss., and impact of culture media on their isolation.azadirachta indica a. juss. (neem), native to india, is well known worldwide for its insecticidal and ethanopharmacological properties. although endophytic microbes are known from this plant as only leaves and stems were the subjects of past reports. now, a variety of procedures and a number of different media were used to isolate the maximum number of endophytic fungi from unripe fruits and roots. a total of 272 isolates of 29 filamentous fungal taxa were isolated at rate of 68.0% from 400 samp ...201123024409
phylogenomic and functional domain analysis of polyketide synthases in fusarium.fusarium species are ubiquitous in nature, cause a range of plant diseases, and produce a variety of chemicals often referred to as secondary metabolites. although some fungal secondary metabolites affect plant growth or protect plants from other fungi and bacteria, their presence in grain-based food and feed is more often associated with a variety of diseases in plants and in animals. many of these structurally diverse metabolites are derived from a family of related enzymes called polyketide s ...201122289777
preparation and characterization of dispersible chitosan particles with borate crosslinking and their antimicrobial and antifungal activity.we synthesized new dispersive chitosan particles at circumneutral ph. particles composed of a chitosan-borate complex were synthesized by a method consisting of two simple steps: mixture and dialysis. as this method does not employ reagents such as organic solvents or surface-active agents and does not require heat treatment, it has a minimal negative impact on the environment. crosslinking of the reaction of glucose and boric acid at ordinary temperature and pressure led to the formation of com ...201122261277
inhibitory effects of essential oils of medicinal plants from growth of plant pathogenic fungi.plant cells produce a vast amount of secondary metabolites. production of some compounds is restricted to a single species. some compounds are nearly always found only in certain specific plant organs and during a specific developmental period of the plant. some secondary metabolites of plants serve as defensive compounds against invading microorganisms. nowadays, it is attempted to substitute the biological and natural agents with chemically synthesized fungicides. in the present research, the ...201122702190
experimental model of fusarium solani keratitis in rats.to establish a repeatable rat model of fusarium solani keratitis (f. solani keratitis) that mimicked fungal keratitis in humans.201122553683
effects of cox-2 inhibitor ns-398 on il-10 expression in rat fungal keratitis.to investigate the expression of interleukin-10 (il-10) and the effect of ns-398(cox-2 inhibitor) on the expression of il-10 in fungal keratitis in rats, and analyze its effects on anti-fungus immunity.201122553634
kinetics of fungal extracellular alpha-amylase from fusarium solani immobilized in calcium alginate beads.extracellular alpha-amylase mass produced by fusarium solani using mango kernel as substrate was immobilized in calcium alginate beads through entrapment technique. maximum enzyme immobilization efficiency was achieved in 2 mm size beads formed by 6.5% (w/v) of sodium alginate in 2% (w/v) calcium chloride. the catalytic properties of the immobilized alpha-amylase were compared with that of free enzyme (soluble). the activity yield of the immobilized enzyme was 81% of the free enzyme. the immobil ...201223741795
age-dependent distribution of fungal endophytes in panax ginseng roots cultivated in korea.fungal endophytes were isolated from 1-, 2-, 3-, and 4-year-old ginseng roots (panax ginseng meyer) cultivated in korea. the isolated fungal endophytes were identified based on sequence analysis of the internal transcribed spacer and morphological characterization by microscopic observations. a total of 81 fungal endophytes were isolated from 24 ginseng roots. fungal endophytes were classified into 9 different fungal species and 2 unknown species. ginseng roots that were 1-, 2-, 3-, and 4-years ...201223717135
diversity of fungal endophytes in various tissues of panax ginseng meyer cultivated in korea.endophytic fungi were isolated from various tissues (root, stem, petiole, leaf, and fl ower stalk) of 3- and 4-year-old ginseng plants (panax ginseng meyer) cultivated in korea. the isolated endophytic fungi were identified based on the sequence analysis of the internal transcribed spacer (its), 1-5.8-its 2. a morphological characterization was also conducted using microscopic observations. according to the identification, 127 fungal isolates were assigned to 27 taxa. the genera of phoma, altern ...201223717122
molecular analysis and anticancer properties of two identified isolates, fusarium solani and emericella nidulans isolated from wady el-natron soil in egypt against caco-2 (atcc) cell line.to characterize, identify and investigate the anticancer properties of two new soil fungal isolates, emericella nidulans and fusarium solani isolated from wady el-natron in egypt against colon cancer caco-2 (atcc) cell line.201223569862
in vitro susceptibility of filamentous fungi to copper nanoparticles assessed by rapid xtt colorimetry and agar dilution method.metal nanoparticles and their uses in various aspects have recently drawn a great deal of attention. one of the major applications is that it can be used as an antimicrobial agent. they can be considered in approaches targeted to decrease the harms caused by microorganisms, specifically fungi, threatening the medical and industrial areas. the aim of this study was to investigate the antifungal activity of synthesized copper nanoparticles (cunps) against four filamentous fungi including alternari ...201223518166
nuclease released by verticillium dahliae is a signal for non-host resistance.a dnase released from the fungal pathogen of bean, fusarium solani f. sp. phaseoli (fsph), was previously shown to signal the activation of total disease resistance and activate pathogenesis-related (pr) genes in pea. data in the current study which used the pea-endocarp model to research non-host resistance, indicated that dnase released by verticillium dahliae (vd), pathogenic on potato also has non-host resistance-inducing capabilities in peas. other strains of vd that release dnase are patho ...201223352407
antibacterial and antifungal activities of crude plant extracts from colombian biodiversity.on a global scale, people have used plants to treat diseases and infections, and this has raised interest on the plant biodiversity potencial in the search of antimicrobial principles. in this work, 75 crude n-hexanes, dichloromethane and methanol extracts from the aerial parts of 25 plants belonging to four botanical families (asteraceae, euphorbiaceae, rubiaceae and solanaceae), collected at the natural regional park ucumari (risaralda, colombia), were evaluated for their antibacterial and ant ...201223342508
design, spectral characterization and biological studies of transition metal(ii) complexes with triazole schiff bases.a new series of three biologically active triazole derived schiff base ligands l(1)-l(3) have been synthesized in equimolar reaction of 3-amino-1h-1,2,4-triazole with pyrrol-2-carboxaldehyde, 4-bromo-thiophene-2-carboxaldehyde, and 5-iodo-2-hydroxy benzaldehyde. the prepared schiff bases were used for further complex formation reaction with different metal elements like co(ii), ni(ii), cu(ii) and zn(ii) as chlorides by using a molar ratio of ligand:metal as 2:1. the structure and bonding nature ...201223277183
studies on a rhein-producing endophytic fungus isolated from rheum palmatum l.rheum palmatum l. (chinese rhubarb) is a highly regarded medicinal plant. its dominant active constituents are anthraquinones including emodin, aloe-emodin, rhein, etc. rhein naturally occurs in anthraquinone (1, 3, 8-trihydroxy-6-methyl anthraquinone), which is found in r. palmatum l. and related plants such as rhubarb. it has good antitumor, anti-inflammatory, anticancer, antimicrobial and hemostatic properties. in this study, a total of 14 strains of endophytic fungi were isolated from r. pal ...201223266728
real-time pcr analysis of fungal organisms and bacterial species at peri-implantitis sites.the potential role of fungal organisms and their co-aggregation with either periodontopathogens or opportunistic pathogens at peri-implantitis sites is unknown. the aim of the present study was to qualitatively/quantitatively analyze and correlate fungal organisms and bacterial species at peri-implantitis sites.201527747631
pseudomonas induces salinity tolerance in cotton (gossypium hirsutum) and resistance to fusarium root rot through the modulation of indole-3-acetic acid.abiotic stresses cause changes in the balance of phytohormones in plants and result in inhibited root growth and an increase in the susceptibility of plants to root rot disease. the aim of this work was to ascertain whether microbial indole-3-acetic acid (iaa) plays a role in the regulation of root growth and microbially mediated control of root rot of cotton caused by fusarium solani. seed germination and seedling growth were improved by both nacl and mg2so4 (100 mm) solutions when treated with ...201526587006
molecular mechanisms underlying the close association between soil burkholderia and fungi.bacterial species belonging to the genus burkholderia have been repeatedly reported to be associated with fungi but the extent and specificity of these associations in soils remain undetermined. to assess whether associations between burkholderia and fungi are widespread in soils, we performed a co-occurrence analysis in an intercontinental soil sample collection. this revealed that burkholderia significantly co-occurred with a wide range of fungi. to analyse the molecular basis of the interacti ...201525989372
volatile organic compounds produced by the phytopathogenic bacterium xanthomonas campestris pv. vesicatoria 85-10.xanthomonas campestris is a phytopathogenic bacterium and causes many diseases of agricultural relevance. volatiles were shown to be important in inter- and intraorganismic attraction and defense reactions. recently it became apparent that also bacteria emit a plethora of volatiles, which influence other organisms such as invertebrates, plants and fungi. as a first step to study volatile-based bacterial-plant interactions, the emission profile of xanthomonas c. pv. vesicatoria 85-10 was determin ...201222563356
diversity, distribution, and antagonistic activities of rhizobacteria of panax notoginseng.rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. this study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of panax notoginseng.201527158229
correlations between root-associated microorganisms and peach replant disease symptoms in a california soil.replant disease often occurs when certain crops are "replanted" in a soil that had previously supported the same or similar plant species. this disease typically leads to reductions in plant growth, crop yields, and production duration, and its etiology remains ill-defined. the objective of this study was to identify microorganisms associated with peach replant disease symptoms at a field location in california, usa. soil samples were subjected to treatments to create various levels of replant d ...201223071565
responses of beneficial bacillus amyloliquefaciens sqr9 to different soilborne fungal pathogens through the alteration of antifungal compounds production.bacillus amyloliquefaciens sqr9 exhibited predominantly antagonistic activities against a broad range of soilborne pathogens. the fungi-induced sqr9 extracts possess stronger antifungal activities compared with sqr9 monoculture extracts. to investigate how sqr9 fine-tunes lipopeptides (lps) and a siderophore bacillibactin production to control different fungal pathogens, lps and bacillibactin production and transcription of the respective encoding genes in sqr9 were measured and compared with si ...201425484880
effect of crude plant extracts from some oaxacan flora on two deleterious fungal phytopathogens and extract compatibility with a biofertilizer strain.the antimicrobial activity of 12 plant extracts was tested against the phytopathogens alternaria alternata and fusarium solani. in addition, the compatibility of the extracts toward bacillus liqueniformis, a biofertilizer and a non-target microorganism, was assessed. plants tested belong to the euphorbiaceae, asteraceae, crassulaceae, rubiaceae, convolvulaceae, verbenaceae, orchidaceae, nyctaginaceae, boraginaceae, and tiliaceae families and were collected in the state of oaxaca. the antifungal ...201425147544
analysis of phaseolus vulgaris response to its association with trichoderma harzianum (all-42) in the presence or absence of the phytopathogenic fungi rhizoctonia solani and fusarium solani.the present study was carried out to evaluate the ability of trichoderma harzianum (all 42-isolated from brazilian cerrado soil) to promote common bean growth and to modulate its metabolism and defense response in the presence or absence of the phytopathogenic fungi rhizoctonia solani and fusarium solani using a proteomic approach. t. harzianum was able to promote common bean plants growth as shown by the increase in root/foliar areas and by size in comparison to plants grown in its absence. the ...201424878929
development of a selective medium for the fungal pathogen cylindrocarpon destructans using radicicol.the soil-borne ascomycete fungus cylindrocarpon destructans causes ginseng root rot disease and produces various secondary metabolites such as brefeldin a and radicicol. the slow growth of this fungus compared with other plant pathogenic and saprophytic fungi in soil disturbs isolation of this fungus from soil and infected ginseng. in this study, we developed a selective medium for c. destructans using radicicol produced by this fungus. supplementing 50 mg/l of radicicol to medium inhibited the ...201425506308
antimicrobial constituents of the leaves of mikania micrantha h. b. k.to isolate plant-derived compounds with antimicrobial activity from the leaves of mikania micrantha, to determine the compounds configuration, and to evaluate their antimicrobial activity against eight plant pathogenic fungi (exserohilum turcicum, colletotrichum lagenarium, pseudoperonispora cubensis, botrytis cirerea, rhizoctonia solani, phytophthora parasitica, fusarium solani, and pythium aphanidermatum,) and four plant pathogenic bacteria (gram negative bacteria: ralstonia dolaanacearum, xan ...201324098556
in the aftermath of the fusarium keratitis outbreak: what have we learned?the 2005-2006 outbreak of fusarium keratitis associated with soft hydrophilic contact lens wear was both unprecedented and unexpected. more than 250 cases have been reported worldwide that have primarily been associated with bausch & lomb renu with moistureloc and, more recently, with bausch and lomb renu multiplus multipurpose contact lens disinfecting solutions. this article documents the outbreak, presenting the time line and the historical and scientific basis for its occurrence. underlying ...200719668512
how often do they have sex? a comparative analysis of the population structure of seven eukaryotic microbial pathogens.the model of predominant clonal evolution (pce) proposed for micropathogens does not state that genetic exchange is totally absent, but rather, that it is too rare to break the prevalent pce pattern. however, the actual impact of this "residual" genetic exchange should be evaluated. multilocus sequence typing (mlst) is an excellent tool to explore the problem. here, we compared online available mlst datasets for seven eukaryotic microbial pathogens: trypanosoma cruzi, the fusarium solani complex ...201425054834
assessment of phytochemicals, antimicrobial and cytotoxic activities of extract and fractions from fagonia olivieri (zygophyllaceae).in pakistan fagonia olivieri (zygophyllaceae) is commonly used in the indigenous system of medicine for treatment of conditions like diabetes, cancer, fever, asthma, toothache, stomach troubles and kidney disorders. this study evaluated the crude methanol extract of f. olivieri (fom) and its derived fractions for their antimicrobial and cytotoxic activities as well as the classes of phytochemical.201323842440
expression of an engineered heterologous antimicrobial peptide in potato alters plant development and mitigates normal abiotic and biotic responses.antimicrobial cationic peptides (amps) are ubiquitous small proteins used by living cells to defend against a wide spectrum of pathogens. their amphipathic property helps their interaction with negatively charged cellular membrane of the pathogen causing cell lysis and death. amps also modulate signaling pathway(s) and cellular processes in animal models; however, little is known of cellular processes other than the pathogen-lysis phenomenon modulated by amps in plants. an engineered heterologou ...201324147012
l-asparaginase activity of fungal endophytes from tabernaemontana heyneana wall. (apocynaceae), endemic to the western ghats (india)."endophytes," the microbes residing within the plant tissues, are important sources of secondary metabolites. tabernaemontana heyneana wall., a medicinal tree, endemic to the western ghats with rich ethnobotanical history and unique chemical diversity, was selected to study fungal endophytes and evaluate them for l-asparaginase activity. healthy plant parts were selected for the isolation of endophytes following standard isolation protocols. a total of 727 isolates belonging to 20 taxa were obta ...201427382605
black cobra (naja naja karachiensis) lysates exhibit broad-spectrum antimicrobial activities.it is hypothesized that animals living in polluted environments possess antimicrobials to counter pathogenic microbes. the fact that snakes feed on germ-infested rodents suggests that they encounter pathogenic microbes and likely possess antimicrobials. the venom is used only to paralyze the rodent, but the ability of snakes to counter potential infections in the gut due to disease-ridden rodents requires robust action of the immune system against a broad range of pathogens. to test this hypothe ...201424625321
killing of diverse eye pathogens (acanthamoeba spp., fusarium solani, and chlamydia trachomatis) with alcohols.blindness is caused by eye pathogens that include a free-living protist (acanthamoeba castellanii, a. byersi, and/or other acanthamoeba spp.), a fungus (fusarium solani), and a bacterium (chlamydia trachomatis). hand-eye contact is likely a contributor to the spread of these pathogens, and so hand washing with soap and water or alcohol-based hand sanitizers (when water is not available) might reduce their transmission. recently we showed that ethanol and isopropanol in concentrations present in ...201728182670
status of the effectiveness of contact lens solutions against keratitis-causing pathogens.the aim of this study was to assess the antimicrobial effects of marketed contact lens disinfecting solutions.201525280981
broad spectrum antimicrobial activity of melimine covalently bound to contact lenses.to develop a stable antimicrobial contact lens, which is effective against the international organization for standardization (iso) panel microorganisms, acanthamoeba castellanii and drug resistant strains of pseudomonas aeruginosa and staphylococcus aureus.201323211820
hexacyclopeptides secreted by an endophytic fungus fusarium solani n06 act as crosstalk molecules in narcissus tazetta.the basis of chemical crosstalk in plants and associated endophytes lies in certain so-called communication molecules that are responsible for plant-microbe and microbe-microbe interactions. consequently, elucidating the factors that affect the nature, distribution, and amount of these molecules and how they impact the interaction among endophytes and associated organisms is essential to understand the true potential of endophytes. in the present study, we report the discovery of nine hexacyclop ...201525958995
[mycetomas diagnosed in senegal from 2008 to 2010].mycetomas are inflammatory pseudotumours of subcutaneous and possibly osseous soft fabrics, generally polyfistulas with chronic mode of evolution.201124451559
identification of the non-ribosomal peptide synthetase responsible for biosynthesis of the potential anti-cancer drug sansalvamide in fusarium solani.sansalvamide is a cyclic pentadepsipeptide produced by fusarium solani and has shown promising results as potential anti-cancer drug. the biosynthetic pathway has until now remained unidentified, but here we used an agrobacterium tumefaciens-mediated transformation (atmt) approach to generate knockout mutants of two candidate non-ribosomal peptide synthetases (nrps29 and nrps30). comparative studies of secondary metabolites in the two deletion mutants and wild type confirmed the absence of sansa ...201626936154
ruta montana l. leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease.the aims of this study were to assess the antimicrobial efficacy of the leaf essential oil and the leaf extracts of r. montana against botrytis cinerea, fusarium oxysporum, verticillium dahliae, aspergillus oryzae and fusarium solani. the oil (1.000 µg/disk) and the extracts (1.500 µg/disk) revealed a remarkable antifungal effect against the tested plant pathogenic fungi with a radial growth inhibition percentage of 40.0-80.0 % and 5.0-58.0 %, respectively along with their respective mic values ...201526417353
antimicrobial, antitumor and brine shrimp lethality assay of ranunculus arvensis l. extracts.to investigate the antitumor activity, brine shrimp lethality assay, antibacterial and antifungal activity of methanol extract (me), water extract (we), acetone extract (ae), chloroform extract (ce), methanol-water extract (mwe), methanol-acetone extract (mae), methanol-chloroform extract (mce) of ranunculus arvensis (l.). antitumor activity was evaluated with agrobacterium tumefaciens (at10) induced potato disc assay. cytotoxicity was evaluated with brine shrimp lethality assay. antibacterial a ...201526004705
synthesis and structure-activity relationship of n-(cinnamyl) chitosan analogs as antimicrobial agents.the current study focuses on the preparation of new n-(cinnamyl) chitosan derivatives as antimicrobial agents against nine types of crop-threatening pathogens. chitosan was reacted with a set of aromatic cinnamaldehyde analogs by reductive amination involving formation of the corresponding imines, followed by reduction with sodium borohydride to produce n-(cinnamyl) chitosan derivatives. the structural characterization was confirmed by (1)h and (13)c nmr spectroscopy and the degrees of substitut ...201323511055
a phenazine-1-carboxylic acid producing polyextremophilic pseudomonas chlororaphis (mcc2693) strain, isolated from mountain ecosystem, possesses biocontrol and plant growth promotion abilities.the genus pseudomonas is known to comprise a huge diversity of species with the ability to thrive in different habitats, including those considered as extreme environments. in the present study, a psychrotolerant, wide ph tolerant and halotolerant strain of pseudomonas chlororaphis gbpi_507 (mcc2693), isolated from the wheat rhizosphere growing in a mountain location in indian himalayan region (ihr), has been investigated for its antimicrobial potential with particular reference to phenazine pro ...201627394000
evaluation of antifungal efficacy of 0.1% and 0.25% riboflavin with uva: a comparative in vitro study.antifungal efficacy of photochemical cross-linking (pack-cxl) with 0.1% and 0.25% riboflavin was evaluated with a comparative in vitro study.201626644282
a single-step purification of cauliflower lysozyme and its dual role against bacterial and fungal plant pathogens.a novel lysozyme from cauliflower was purified in a single step, for the first time, using sephadex g100 column chromatography. the purified lysozyme exhibited a homogenized single band in sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), and its molecular mass was calculated to be 22.0 kda. the purified lysozyme showed activity between 30 to 60 °c with 40 °c as the optimum temperature for its maximal activity. although the purified lysozyme was functional at ph ranges betwee ...201526208688
effect of elicitors and precursors on azadirachtin production in hairy root culture of azadirachta indica.the present study involved strategies for enhancement in in vitro azadirachtin (commercially used biopesticide) production by hairy root cultivation of azadirachta indica. improvement in the azadirachtin production via triggering its biosynthetic pathway in plant cells was carried out by the exogenous addition of precursors and elicitors in the growth medium. among the different abiotic stress inducers (ag(+), hg(+2), co(+2), cu(+2)) and signal molecules (methyl jasmonate and salicylic acid) tes ...201424357500
isolation and characterization of rhizosphere bacteria for the biocontrol of the damping-off disease of tomatoes in tunisia.fluorescent pseudomonas spp., isolated from tomato and pepper plants rhizosphere soil, was evaluated in vitro as a potential antagonist of fungal pathogens. pseudomonas strains were tested against the causal agents of tomatoes damping-off (sclerotinia sclerotiorum), root rot (fusarium solani), and causal agents of stem canker and leaf blight (alternaria alternata). for this purpose, dual culture antagonism assays were carried out on 25% tryptic soy agar, king b medium and potato dextrose agar to ...201624296079
in vitro antifungal activity of silver nanoparticles against ocular pathogenic filamentous fungi.fungal keratitis is emerging as a major cause of vision loss in a developing country such as china because of higher incidence and the unavailability of effective antifungals. it is urgent to explore broad-spectrum antifungals to effectively suppress ocular fungal pathogens, and to develop new antifungal eye drops to combat this vision-threatening infection. the aim of this study is to investigate the antifungal activity of silver nanoparticles (nano-ag) in comparison with that of natamycin agai ...201323410063
production of antifungal chitinase by aspergillus niger lock 62 and its potential role in the biological control.aspergillus niger lock 62 produces an antifungal chitinase. different sources of chitin in the medium were used to test the production of the chitinase. chitinase production was most effective when colloidal chitin and shrimp shell were used as substrates. the optimum incubation period for chitinase production by aspergillus niger lock 62 was 6 days. the chitinase was purified from the culture medium by fractionation with ammonium sulfate and affinity chromatography. the molecular mass of the pu ...201222922773
fruit-specific overexpression of wound-induced tap1 under e8 promoter in tomato confers resistance to fungal pathogens at ripening stage.based on high economic importance and nutritious value of tomato fruits and as previous studies employed e8 promoter in fruit ripening-specific gene expression, we have developed transgenic tomato plants overexpressing tomato anionic peroxidase cdna (tap1) under e8 promoter. stable transgene integration was confirmed by polymerase chain reaction (pcr) and southern analysis for nptii. northern blotting confirmed elevated tap1 levels in the breaker- and red-ripe stages of t(1) transgenic fruits, w ...201222462603
metabolites from aspergillus fumigatus, an endophytic fungus associated with melia azedarach, and their antifungal, antifeedant, and toxic activities.thirty-nine fungal metabolites 1-39, including two new alkaloids, 12β-hydroxy-13α-methoxyverruculogen tr-2 (6) and 3-hydroxyfumiquinazoline a (16), were isolated from the fermentation broth of aspergillus fumigatus ln-4, an endophytic fungus isolated from the stem bark of melia azedarach. their structures were elucidated on the basis of detailed spectroscopic analysis (mass spectrometry and one- and two-dimensional nmr experiments) and by comparison of their nmr data with those reported in the l ...201222409377
streptomycetes and micromycetes as perspective antagonists of fungal phytopathogens.among natural factors that permanently influence on the plants, the soil microorganisms play a special role for the growing of plants as habitants of their rhizosphere. mainly they are the representatives of actinomycetes genus streptomyces and fungal genus penicillium and their metabolic products stimulate plant growth and inhibit the growth of pathogenic fungi and bacteria. the aim of our study was to determine the antagonism of actinomycetes and micromycetes isolated from soils of r. moldova ...201223878981
primary cutaneous cryptococcosis caused by cryptococcus gattii vgii in a tsunami survivor from thailand.skin and soft tissue fungal infections with apophysomyces elegans, fusarium solani, cladophialophora bantiana have been reported in survivors from 2004 indian ocean tsunami. we report the first case of primary cutaneous cryptococcosis caused by cryptococcus gattii vgii in a tsunami survivor from thailand.201425379396
in vitro evaluation of the ovistatic and ovicidal effect of the cosmopolitan filamentous fungi isolated from soil on ascaris suum eggs.the ovicidal activity of seven fungal strains: acremonium alabamense, alternaria chlamydospora, cladosporium herbarum, fusarium solani, paecilomyces variotii, paecilomyces viridis and penicillium verruculosum isolated from urban soil samples from poland was determined in vitro. the fungal mycelium was co-cultured with ascaris suum eggs on plates with 2% water-agar for 28 days. eggs exposed and unexposed (control) to fungal mycelium were observed weekly by light microscopy and the percentage of m ...201424295958
[microecological mechanisms of red-leaf disease occurrence in salvia miltiorrhiza bge].a comparative study was made on the nutrient content in rhizosphere soil and the microflora in rhizosphere soil and on rhizoplane of healthy and red-leaf diseased salvia miltiorrhiza plants, aimed to approach the microecological mechanisms of red-leaf disease occurrence in s. miltiorrhiza. the n, p, k, and mn contents in the diseased plant leaves were significantly lower than those in the healthy plant leaves (p < 0.05). no significant difference was observed in the available p content in the rh ...201324175532
rifampin enhances the activity of amphotericin b against fusarium solani species complex and aspergillus flavus species complex isolates from keratitis patients.the in vitro activities of amphotericin b in combination with rifampin were assessed against 95 ocular fungal isolates. the interactions between amphotericin b and rifampin at 4, 8, 16, and 32 μg/ml were synergistic for 11.8%, 51.0%, 90.2%, and 94.1%, respectively, of fusarium solani species complex isolates and for 13.6%, 45.5%, 93.2%, and 95.5%, respectively, of aspergillus flavus species complex isolates. antagonism was never observed for the amphotericin b-rifampin combinations.201728115342
efficacy of antibody to pnag against keratitis caused by fungal pathogens.developing immunotherapies for fungal eye infections is a high priority. we analyzed fungal pathogens for expression of the surface polysaccharide, poly-n-acetyl glucosamine (pnag), and used a mouse model of ocular keratitis caused by aspergillus flavus, a. fumigatus, or fusarium solani to determine if pnag was an immunotherapy target and requirements for ancillary cellular and molecular immune effectors.201628002842
antimicrobial metal-based thiophene derived compounds.a novel series of thiophene derived schiff bases and their transition metal- [co(ii), cu(ii), zn(ii), ni(ii)] based compounds are reported. the schiff bases act as tridentate ligands toward metal ions via azomethine-n, deprotonated-n of ammine substituents and s-atom of thienyl moiety. the synthesized ligands along with their metal complexes were screened for their in vitro antibacterial activity against six bacterial pathogens (escherichia coli, shigella flexneri, pseudomonas aeruginosa, salmon ...201727766891
discrimination of aspergillosis, mucormycosis, fusariosis, and scedosporiosis in formalin-fixed paraffin-embedded tissue specimens by use of multiple real-time quantitative pcr assays.in a retrospective multicenter study, 102 formalin-fixed paraffin-embedded (ffpe) tissue specimens with histopathology results were tested. two 4- to 5-μm ffpe tissue sections from each specimen were digested with proteinase k, followed by automated nucleic acid extraction. multiple real-time quantitative pcr (qpcr) assays targeting the internal transcribed spacer 2 (its2) region of ribosomal dna, using fluorescently labeled primers, was performed to identify clinically important genera and spec ...201627605714
metal-based ethanolamine-derived compounds: a note on their synthesis, characterization and bioactivity.metal-based ethanolamines, (l(1))-(l(4)) coordinated with co(ii), cu(ii), ni(ii) and zn(ii) metals in 1:2 (metal:ligand) molar ratio to produce new compounds have been reported. these compounds were screened for their bactericidal/fungicidal activity against a number of bacterial (escherichia coli, shigella flexneri, pseudomonas aeruginosa, salmonella typhi, staphylococcus aureus and bacillus subtilis) and fungal strains (trichophyton longifusus, candida albicans, aspergillus flavus, microsporum ...201627557038
antimicrobial potential of consolidation polymers loaded with biological copper nanoparticles.biodeterioration of historic monuments and stone works by microorganisms takes place as a result of biofilm production and secretion of organic compounds that negatively affect on the stone matrix.201627400968
fungal infections after boston type 1 keratoprosthesis implantation: literature review and in vitro antifungal activity of hypochlorous acid.to review the current literature describing cases of fungal keratitis and endophthalmitis after boston keratoprosthesis (kpro) implantation and to characterize the antifungal activity of 0.01% hypochlorous acid against medically relevant fungi.201526488624
sertraline enhances the activity of antimicrobial agents against pathogens of clinical relevance.serotonin reuptake inhibitors were recently reported to possess antimicrobial potentials, potentiate activity of several antibiotics, reverse multidrug resistant phenotypes of bacteria and make them susceptible to previously resistant drugs. we investigated antimicrobial potentials of sertraline (sr) against atcc strains, clinical isolates of staphylococcus aureus, escherichia coli and pseudomonas aeruginosa alone and in-combination with seven antibiotics. antifungal activity was investigated ag ...201526029671
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