| proteins of the bacillus stearothermophilus ribosome. crystallization of proteins l30 and s5. | proteins l30 and s5 from the 50 s and 30 s subunits, respectively, of the bacillus stearothermophilus ribosome have been crystallized. l30 crystals are tetragonal and the space group is p4(1)2(1)2 (or p4(3)2(1)2) with cell dimensions a = b = 46.3 a and c = 61.4 a. s5 crystals are trigonal with the space group p3(1)21 (or p3(2)21) and cell dimensions a = b = 59.3 a and c = 109.8 a. in both cases, there appears to be a single molecule in the asymmetric unit. | 1983 | 6630234 |
| [preparation of bioindicators for controlling the efficacy of sterilization processes]. | a test microbe for the control of the efficacy of vacuum steam sterilization was selected. conditions for the spore cultivation were developed. the optimal bioindicator composition, test microbe loading, nutrient medium, ph indicator, carbohydrates and a carrier were defined. | 1983 | 6638973 |
| resonance raman spectroscopy of azotobacter vinelandii ferredoxin i. vibrational features of the [3fe-3s] cluster. | low temperature resonance raman spectra have been obtained for clostridium pasteurianum and bacillus stearothermophilus ferredoxins. several heretofore undetected fundamental bands have been observed and these data have been used to discriminate the vibrational contribution of the [3fe-3s] cluster to the spectrum of azotobacter vinelandii ferredoxin i. the vibrational features of the [3fe-3s] core distinguish it from other 3-iron clusters and imply structural differences among this class of iron ... | 1983 | 6641938 |
| [electron microscopic study of bacillus stearothermophilus lysis in stab cultivation]. | both vegetative bacillus stearothermophilus cells 3 and cells with mature spores undergo lysis during submerged cultivation in media with starch and maltose as was shown by light and electron microscopy. this can be caused by enzymes lysing cell wall and synthesized at the beginning of the culture growth. the fine structure of the strain 3 spore has certain peculiarities: it contains no exosporium and the inner envelope has a heterogeneous structure. | 1983 | 6645995 |
| nad+ analogue binding to glyceraldehyde-3-phosphate dehydrogenase. | a series of nad+ analogues, modified on the pyridinium ring, have been tested for their enzymic properties in reactions with d-glyceraldehyde-3-phosphate dehydrogenase form sturgeon muscle, rabbit muscle and bacillus stearothermophilus. the observed activity, inhibition and binding data are correlated to the structure of the enzyme and coenzyme analogue by model building on a vector general interactive graphic display system using coordinates from the b. stearothermophilus holoenzyme structure. ... | 1983 | 6653561 |
| comparative thermoresistance of two biological indicators for monitoring steam autoclaves. i. comparison performed in a gravity bier/steam vessel. | the thermoresistance of two biological indicators (bis) for steam sterilization control (swedish spore tablets and attest no. 1242 bi) was compared at 121 degrees c and at 134 degrees c, saturated steam, in a special test autoclave (a gravity bier/steam vessel). in this autoclave, three lots of attest no. 1242 bi (abi) demonstrated longer survival exposure times than did the three lots of the scandinavian bi (sbi) tested while the survival-kill range was approx. the same. also, the abi had highe ... | 1983 | 6673502 |
| [semiquantitative determination of leukocyturia, a necessary complement to urine culture]. | the authors used a strip-test (cytur-test) for the semiquantitative assay of leukocytes in the urines: they also did a cultural examination and an assay of antimicrobial power (par-test). or the whole of 2613 urines they found 931 specimens (35.6%) with a number of leukocytes higher than 10/microliters; the 44.7% of these came out negative of the bacterial research. the cytur-test proved to be an important method in the diagnosis of pathologic leukocyturia in the screening of urines because of i ... | 1983 | 6675044 |
| amino acid sequence of ovine 6-phosphogluconate dehydrogenase. | the amino acid sequence of the nadp+-dependent enzyme ovine 6-phosphogluconate dehydrogenase has been determined by conventional direct protein sequence analysis of peptides resulting from digestion of the protein with trypsin and chemical cleavages with cyanogen bromide, hydroxylamine, and iodosobenzoic acid. the polypeptide contains 466 amino acids and its nh2 terminus is acetylated. the candida utilis enzyme is inactivated by reaction of pyridoxal phosphate with two lysine residues (minchiott ... | 1983 | 6685125 |
| a large increase in enzyme-substrate affinity by protein engineering. | a single point mutation has been engineered in the tyrosyl-trna synthetase that improves its affinity (km) for its substrate atp by a factor of 100. in the crystal structure of the tyrosyl trna synthetase (of bacillus stearothermophilus), the side-chain hydroxyl of thr 51 appears to make a weak hydrogen bond with the amp moiety of the substrate intermediate, tyrosyl adenylate. in the absence of substrate, however, the hydroxyl group should make a strong hydrogen bond with water which would favou ... | 1984 | 6690998 |
| two-dimensional crystalline sheets of bacillus stearothermophilus 50s ribosomal particles. | two-dimensional crystalline sheets of the large ribosomal subunit from bacillus stearothermophilus have been obtained using a slightly modified procedure to that for growing three-dimensional crystals of the same material. the crystalline subunits are packed within monolayers in a relatively small unit cell, the dimensions of which are closely related to those observed for two forms of the three-dimensional crystals. the packing symmetry is p121, and the optical diffraction patterns of micrograp ... | 1984 | 6705764 |
| bacillus stearothermophilus disk assay detection of penicillin in milk of dairy cows after postestrual intrauterine infusion. | penicillin concentrations were quantitated in milk from cows after intrauterine infusions of various amounts of penicillin. six healthy lactating holsteins were assigned to 3 treatment groups in a complete randomized block design. postestrual (12 to 48 hours) intrauterine infusions of potassium penicillin g in sterile diluent were given at 1 of 3 dosage levels: 2 x 10(6) iu (group i), 1.5 x 10(6) iu (group ii), or 1 x 10(6) iu (group iii). milk samples were collected from 0 to 72 hours after inf ... | 1984 | 6711967 |
| optimization of autoclave cycles and selection of formulation for parenteral products, part iii: effect of formulation variables on sporicidal kinetics. | | 1984 | 6726544 |
| evidence for the glycoprotein nature of the crystalline cell wall surface layer of bacillus stearothermophilus strain nrs2004/3a. | the surface layer of bacillus stearothermophilus strain nrs2004/3a was isolated and chemically characterized. the results of these initial studies lead to the conclusion that the cell surface protein is glycosylated. | 1984 | 6745426 |
| some x-ray diffraction patterns from single crystals of the large ribosomal subunit from bacillus stearothermophilus. | x-ray diffraction patterns of three-dimensional crystals of the large ribosomal subunit from bacillus stearothermophilus have been obtained using a synchrotron radiation beam. the patterns contain resolved diffraction spots and indicate packing in relatively small unit cells, the dimensions of which have been tentatively determined. the internal order of the crystals, as reflected in these patterns, has been correlated with the size, shape and conditions of growth of these crystals. | 1984 | 6748083 |
| cloning and amplified expression of the tyrosyl-trna synthetase genes of bacillus stearothermophilus and escherichia coli. | two fragments of dna which carry the genes coding for the tyrosyl-trna synthetases of escherichia coli and bacillus stearothermophilus have been cloned into the plasmid pbr322 and were selected by complementation of an e. coli temperature-sensitive mutant. transformation of this strain with either of the recombinant plasmids results in a 100-fold increase in tyrosyl-trna synthetase activity measured in vitro and the protein products co-migrate with the corresponding purified enzymes on polyacryl ... | 1982 | 6749496 |
| conserved cysteine and histidine residues in the structures of the tyrosyl and methionyl-trna synthetases. | | 1982 | 6751870 |
| the potential for contamination: a pilot study of open and closed surgical gloving. | | 1982 | 6756303 |
| use of the carbon dioxide laser in sterilization of endodontic reamers. | the object of this study was to test a new method of sterilizing endodontic instruments by using a carbon dioxide laser system. this was done by contaminating endodontic reamers with spores, exposing them to a co2 laser beam, and checking for their viability by standard microbiologic techniques. it was found that 100 percent of the spores were killed by the co2 laser. this holds promise as an effective method of sterilizing endodontic instruments in the future. | 1980 | 6766545 |
| d-glyceraldehyde-3-phosphate dehydrogenase. amino-acid sequence of the enzyme from the extreme thermophile thermus aquaticus. | 1. the amino acid sequence of d-glyceraldehyde-3-phosphate dehydrogenase from the extreme thermophile thermus aquaticus has been elucidated. 2. the polypeptide contains 332 amino acids and its sequence is 70% identical with that of the enzyme from the moderate thermophile bacillus stearothermophilus. 3. in contrast to less thermostable forms of the enzymes from b. stearothermophilus, pig, lobster and yeast, the t. aquaticus enzyme has only one cysteine residue, namely cysteine-149 which is requi ... | 1980 | 6773768 |
| limited proteolysis of the penicillin-sensitive d-alanine carboxypeptidase purified from bacillus subtilis membranes. active water-soluble fragments generated by cleavage of a cooh-terminal membrane anchor. | | 1981 | 6780558 |
| primary structure of the cooh-terminal membranous segment of a penicillin-sensitive enzyme purified from two bacilli. | d-alanine carboxypeptidase is a penicillin-sensitive intrinsic membrane enzyme which is composed of a hydrophilic nh2-terminal catalytic domain (mr congruent to 45,000 to 47,000) and a cooh-terminal membranous segment (approximately 20 to 30 amino acids in length) (waxman, d. j., and strominger, j. l. (1979) j. biol. chem. 254, 4863-4875; waxman, d. j., and strominger, j. l. (1981) j. biol. chem. 256, 2059-2066). the primary structures of the cooh-terminal 30 amino acids of two d-alanine carboxy ... | 1981 | 6780559 |
| properties of the virulent form of a mitomycin c- or temperature-induced thermophilic bacteriophage. | a virulent bacteriophage was isolated from lysates of bacillus stearothermophilus strain nu-10 induced either by treatment with mitomycin c or by shifts in temperature. optimum conditions for induction, morphology and other properties of the virus are described. chloroform treatment and shifts in temperature of producer cells released approximately similar amounts of phage as did mitomycin induction, suggesting an effect on release rather than on synthesis of the virus. | 1981 | 6790667 |
| monitoring steam sterilization of surgical instruments: a dilemma. | the study of two biological indicators in monitoring "flash" sterilization demonstrated that indicator construction often leads to a false interpretation of spore survival. | 1981 | 6792986 |
| binding of zinc to escherichia coli phenylalanyl transfer ribonucleic acid synthetase. comparison with other aminoacyl transfer ribonucleic acid synthetases. | | 1981 | 6794600 |
| photometric immersion refractometry of bacterial spores. | photometric immersion refractometry was used to determine the average apparent refractive index (n) of five types of dormant bacillus spores representing a 600-fold range in moist-heat resistance determined as a d100 value. the n of a spore type increased as the molecular size of various immersion solutes decreased. for comparison of the spore types, the n of the entire spore and of the isolated integument was determined by use of bovine serum albumin, which is excluded from permeating into them ... | 1982 | 6802796 |
| bacterial spore heat resistance correlated with water content, wet density, and protoplast/sporoplast volume ratio. | five types of dormant bacillus spores, between and within species, were selected representing a 600-fold range in moist-heat resistance determined as a d100 value. the wet and dry density and the solids and water content of the entire spore and isolated integument of each type were determined directly from gram masses of material, with correction for interstitial water. the ratio between the volume occupied by the protoplast (the structures bounded by the inner pericytoplasm membrane) and the vo ... | 1982 | 6802802 |
| ultrasonic potentiation of the sporicidal activity of glutaraldehyde. | laboratory test data showed a 4.8-fold increase in the sporicidal activity of glutaraldehyde with the incorporation of ultrasound. projected times required for 100 percent killed of a 20,000 hydrated spore inoculant were reduced from 211 minutes to 44 minutes. clinical exposure time intervals necessitated a 31/2-hour glutaraldehyde immersion versus a 30-minute cavitated glutaraldehyde chemosterilization to ensure a 100 percent kill. the feasibility of reducing the ada-required 10-hour soak in gl ... | 1982 | 6804906 |
| reconstitution of the pyruvate dehydrogenase multienzyme complex from bacillus stearothermophilus. | | 1982 | 6810922 |
| patterns of nucleotide utilisation in bacterial succinate thiokinases. | | 1982 | 6811321 |
| redesigning enzyme structure by site-directed mutagenesis: tyrosyl trna synthetase and atp binding. | | 1982 | 6811955 |
| pyruvate dehydrogenase complex from bacillus. | | 1982 | 6815422 |
| a method for measuring the cleaning effect of flushing disinfectors. | a method is presented with which the mechanical cleaning effect of flushing disinfectors can be estimated independently of the thermal disinfecting effect of the hot flushing water. this makes it possible to specify the demands to be placed on the disinfecting effect of flushing with water of 85 degrees c or more. bacillus stearothermophilus spores suspended in faeces were used as indicators because of their non-sensitivity to the hot-water temperature. their elimination by flushing could thus b ... | 1980 | 6820026 |
| several crystal forms of the bacillus stearothermophilus 50 s ribosomal particles. | | 1983 | 6832362 |
| products of phospholipid metabolism in bacillus stearothermophilus. | the products of phospholipid turnover in bacillus stearothermophilus were determined in cultures labeled to equilibrium and with short pulses of [32p]phosphate and [2-3h]glycerol. label lost from the cellular lipid pool was recovered in three fractions: low-molecular-weight extracellular products, extracellular lipid, and lipoteichoic acid (lta). the low-molecular-weight turnover products were released from the cells during the first 10 to 20 min of a 60-min chase period and appeared to be deriv ... | 1983 | 6833180 |
| the amino acid sequence of the tyrosyl-trna synthetase from bacillus stearothermophilus. | the primary structure of the tyrosyl-trna synthetase (tyrts) of bacillus stearothermophilus has been deduced from the nucleotide sequence of the cloned gene and from the amino acid sequence of peptides isolated from the purified enzyme. tyrts (b. stearothermophilus) has a molecular weight of 47316 and the sequence is 56% homologous with that of tyrts (escherichia coli). the binding domain for the substrate intermediate tyrosyl adenylate is located in the n-terminal portion of the polypeptide and ... | 1983 | 6840095 |
| coenzyme binding in crystals of glyceraldehyde-3-phosphate dehydrogenase. | apo-glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus and the partially saturated holo-enzyme can be crystallized isomorphously with the entire tetramer occupying the crystal asymmetric unit. for crystals that contain one molecule of nad+ per tetramer the coenzyme is bound uniquely in one of the four available sites. the presence of nad+ gives rise to nonequivalence in the binding of a heavy-atom compound to the subunits of the tetramer while for the apo-enzyme this bindi ... | 1983 | 6842606 |
| an evaluation of the charm test--a rapid method for the detection of penicillin in milk. | using the charm radioactive assay technique as a rapid test for the determination of penicillin in milk in experiments with milks containing known concentrations of penicillin, the coefficient of variation was found to be 15 and 11% for raw whole and reconstituted skim-milks respectively. storage of reagents for up to 4 d increased the variance of the test by more than 20% and the estimated penicillin concentration 0.4%/d of storage up to 25 d. in comparison with the bromocresol purple test, whi ... | 1983 | 6863689 |
| proteins of the bacillus stearothermophilus ribosome. a low resolution crystal analysis of protein l30. | the 5 a resolution crystal structure analysis of ribosomal protein l30 from bacillus stearothermophilus is described. the molecule is shown to be compact and extend to about 25-30 a in each dimension. | 1983 | 6884515 |
| proteins of the bacillus stearothermophilus ribosome. crystallization of protein l6. | crystals of ribosomal protein l6 from bacillus stearothermophilus suitable for high resolution structural studies have been obtained. crystals are hexagonal with space group p6122 (or the enantiomorph p6522) and cell dimensions a = b = 72.7 a, c = 124.9 a. a search for heavy atom derivatives is in progress. | 1983 | 6884516 |
| the primary structure of initiation factor if3 from bacillus stearothermophilus. | the complete amino acid sequence of the initiation factor if3 from bacillus stearothermophilus has been elucidated. this was achieved by splitting the protein with trypsin, staphylococcus protease or cyanogen bromide. the amino acid sequence was determined by manual edman degradation, using the dabitc/pitc double-coupling method. the if3 molecule contains 171 amino acids and has an mr of 19 677. the sequence was compared to the homologous molecule from escherichia coli; about 50% of the amino ac ... | 1983 | 6884517 |
| purification and characterization of the respiratory nitrate reductase of bacillus stearothermophilus. | | 1982 | 6891189 |
| an amino acid sequence in the active site of lipoamide dehydrogenase from bacillus stearothermophilus. | | 1982 | 6896188 |
| [microbiological control of autoclave procedures in odontologic practice]. | | 1980 | 6986603 |
| a simplified procedure for the isolation of bacterial polypeptide elongation factor ef-tu. | | 1980 | 6992647 |
| amino acid:trna ligases (ec 6.1.1..-). | | 1980 | 7000546 |
| stoichiometry and structure of a complex of acidic ribosomal proteins. | | 1980 | 7003159 |
| probing the limits of protein-amino acid side chain recognition with the aminoacyl-trna synthetases. discrimination against phenylalanine by tyrosyl-trna synthetases. | the specificity of the tyrosyl-trna synthetases from escherichia coli and bacillus stearothermophilus for tyrosine compared with phenylalanine has been determined by using samples of phenylalanine which have been scrupulously freed from tyrosine by either chemical or enzymic scavenging procedures. both kinetic measurements and product analyses give a value of 1 x 10(5)-2 x 10(5) for the preferential activation of tyrosine. combined with the known ratio of phenylalanine to tyrosine in rapidly gro ... | 1980 | 7006687 |
| immunochemical evidence of homologies among 50 s ribosomal proteins of bacillus stearothermophilus and escherichia coli. | antibodies prepared against individual 50 s ribosomal subunit proteins from escherichia coli were reacted with 70 s ribosomal proteins from bacillus stearothermophilus in order to identify homologous protein pairs. b. stearothermophilus proteins were separated by two-dimensional polyacrylamide gel electrophoresis and transferred electrophoretically to diazobenzyloxymethyl paper to which they became covalently attached. the paper was then washed with antiserum followed by radioactive protein a, a ... | 1981 | 7024264 |
| alternative pathways for editing non-cognate amino acids by aminoacyl-trna synthetases. | evidence is presented that the editing mechanisms of aminoacyl-trna synthetase operate by two alternative pathways: pre-transfer, by hydrolysis of the non-cognate aminoacyl adenylate; post-transfer, by hydrolysis of the mischarged trna. the methionyl-trna synthetases from escherichia coli and bacillus stearothermophilus and isoleucyl-trna synthetase from e. coli, for example, are shown to reject misactivated homocysteine rapidly by the pre-transfer route. a novel feature of this reaction is that ... | 1981 | 7024910 |
| the crystallization of ribosomal proteins from the 50 s subunit of the escherichia coli and bacillus stearothermophilus ribosome. | several individual intact ribosomal proteins purified from bacterial sources under mild conditions have been crystallized. a number of these are suitable candidates for three-dimensional structural studies by x-ray diffraction techniques. data collection to 3 a resolution for one of these proteins is in progress. | 1981 | 7028741 |
| the l7/l12 stalk, a conserved feature of the prokaryotic ribosome, is attached to the large subunit through its n terminus. | | 1981 | 7028993 |
| the complete amino acid sequence of human muscle glyceraldehyde 3-phosphate dehydrogenase. | | 1981 | 7030790 |
| secondary structure model for 23s ribosomal rna. | a secondary structure model for 23s ribosomal rna has been constructed on the basis of comparative sequence data, including the complete sequences from e. coli. bacillus stearothermophilis, human and mouse mitochondria and several partial sequences. the model has been tested extensively with single strand-specific chemical and enzymatic probes. long range base-paired interactions organize the molecule into six major structural domains containing over 100 individual helices in all. regions contai ... | 1981 | 7031608 |
| intramolecular coupling of active sites in the pyruvate dehydrogenase multienzyme complexes from bacterial and mammalian sources. | a simple method was developed for assessing the intramolecular coupling of active sites in the lipoate acetyltransferase (e2) component of the pyruvate dehydrogenase multienzyme complexes from escherichia coli, bacillus stearothermophilus and ox heart and pig heart mitochondria. samples of enzyme complex were prepared in which the pyruvate decarboxylase (e1) component was selectively and partly inhibited by treatment with increasing amounts of a transition-state analogue, thiamin thio-thiazolone ... | 1981 | 7032507 |
| molecular weight and amino acid composition of a thermostable lytic endopeptidase. | a thermostable lytic endopeptidase from bacillus stearothermophilus 1503-4r was purified 14,500-fold, with a 34% recovery of lytic activity. the enzyme is a basic protein (pi, 9.7) with a molecular weight of 15,100 and is composed of approximately 129 amino acid residues. | 1982 | 7037751 |
| the nucleotide sequence of the structural gene for escherichia coli tryptophanyl-trna synthetase. | the complete nucleotide sequence of trps, the structural gene for escherichia coli tryptophanyl-trna synthetase, was determined using a plasmid carrying the structural gene. from the single open reading frame of correct length and orientation we deduced an amino acid sequence consistent with the amino acid composition of the purified protein. in addition, previously sequenced peptides representing 52% of the protein were readily aligned with regions of the deduced sequence. the deduced amino aci ... | 1982 | 7042706 |
| partition of intermediates of triosephosphate isomerase: slow conformational changes precede enolization and follow product release. | | 1982 | 7044418 |
| [thermal resistance of the spores of a bac. stearothermophilus culture used for the preparation of bioindicators]. | thermostability of the spores of bac. stearothermophilus in ampoules and capillaries in concentrations of 10(9), 10(8) and 10(6) cells per 1 ml of sodium chloride isotonic solution was determined at 119 to 124 degrees c with an interval of 1 degree c and an exposure time of 5, 10, 15, 20, 25, 30, 35 and 40 minutes. the results were used for plotting the survival curves. the time of the microbial death in the ampoules and capillaries at all the temperatures was the same and the ampoules were chos ... | 1982 | 7065645 |
| influence of environmental storage relative humidity on biological indicator resistance, viability, and moisture content. | the effect of environmental storage relative humidity (rh) on the moisture content, viability, and moist heat and gaseous ethylene oxide (eo) resistance of biological indicators (bis) was evaluated. no statistically significant difference was observed between the initial bacillus stearothermophilus spore population and the spore population of bis stored at 20 degrees c and 0, 20, 44, of 55% rh or under ambient, 4 degrees c, or -20 degrees c conditions after 12 months. a statistically significant ... | 1982 | 7073275 |
| the reactivation of stationary phase ribosomes from bacillus stearothermophilus. | | 1982 | 7073751 |
| crystallization of lactate dehydrogenase from bacillus stearothermophilus. | | 1982 | 7077664 |
| a new approach to sterilization conditions. the imo concept. | the sterilization requirements for medical/pharmaceutical applications are traditionally based on an extensive overkill. in the past few years, however, an evolution towards bioburden related sterilization processes has been started (f0 theory). especially manufacturers of large volume parenterals have - forced by the thermolability of the product - contributed to this development. in this paper both philosophies are combined, resulting in a concept in which the bacteriological and physical base ... | 1982 | 7099915 |
| [numerical taxonomy of a thermophilic "bacillus" species isolated from west african rice soils (author's transl)]. | fifty-seven strains of endospore-forming thermophilic bacteria, 37 of which were capable of denitrification, were isolated from rice soils of west africa. they were compared with 17 strains of similar bacteria from culture collections, utilizing a total of 123 morphological, physiological and biochemical characteristics. a numerical analysis was performed using the complete linkage-clustering method and the khi2 test. seventy-five percent (55 strains) could be included in 12 groups at a taxonomi ... | 1982 | 7103312 |
| mössbauer spectroscopic evidence for the conversion of [4 fe--4 s] clusters in bacillus stearothermophilus ferredoxin into [3 fe--3 s] clusters. | | 1982 | 7106280 |
| the interaction of tryptophanyl-trna synthetase with the triazine dye brown mx-5br. | brown mx-5br specifically and irreversibly inactivates tryptophanyl-trna synthetase from bacillus stearothermophilus at ph 8.5. the enzyme is protected from inactivation by the substrates tryptophan and atp and to lesser extents by adp, amp, the product inorganic pyrophosphate and other nucleotides such as gtp. the kd of the pure reactive dye for the enzyme was measured to be 6.7 x 10(-6) m. the km values of the two substrates tryptophan and mgatp were found to be 1 x 10(-5) m and 5 x 10(-5) m r ... | 1982 | 7117237 |
| effect of environmental conditions during heating on commercial spore strip performance. | commercial biological indicator spore strips in glassine envelopes, produced by three manufacturers, were evaluated by fraction-negative procedures after being heated at 121.0 +/- 0.05 degrees c. only one type of spore strip met the manufacturer's specifications. the strips of one manufacturer were further evaluated by fraction-negative and survivor curve-plate count procedures after being heated under several conditions (enclosed in glassine envelopes, in trypticase soy broth plus 0.0015% bromo ... | 1982 | 7125646 |
| rapid screening assay for beta-lactam antibiotics in milk: collaborative study. | a 15 min assay for beta-lactam antibiotics has been used by dairies for several years as a screening procedure for testing milk tankers before they unload. the test is based on a competition between 14c-penicillin and beta-lactam antibiotics in the milk samples for sites on a microbial cell wall that specifically binds beta-lactam. in a collaborative study, 11 laboratories correctly distinguished 10 coded zero penicillin g samples and 10 coded 0.01 iu/ml samples. the proposed test is qualitative ... | 1982 | 7130091 |
| qualitative ampule and multitest for beta-lactam residues in fluid milk products: collaborative study. | a collaborative study was performed on a rapid bacillus stearothermophilus agar diffusion ampule method to detect low levels of penicillin g in 7 types of fluid milk products. a multitest technique for processing a large number of samples simultaneously was also studied. slight modifications were made in the original method to establish more uniformity and to eliminate doubtful responses by specifying a confirmation procedure. twenty samples spiked with penicillin g (0.000 to 0.008 iu/ml) and te ... | 1982 | 7130092 |
| bacillus stearothermophilus disc assay for detection of inhibitors in milk: collaborative study. | a 2-part (a and b) collaborative study was conducted on a bacillus stearothermophilus paper disc (12.7 mm) method to detect residual inhibitors in milk. the 18 participating collaborators assayed raw milk samples spiked with a beta-lactam (penicillin g). of the 18 collaborators, 14 participated in part a and 16 in part b. part a demonstrated that either antibiotic medium no. 4 or pm indicator agar is suitable for use in the assay. the lowest concentration detectable, not significantly different ... | 1982 | 7130093 |
| use of chromatofocusing in the purification of tryptophanyl-trna synthetase from bacillus stearothermophilus. | | 1982 | 7149233 |
| phosphoglycerate kinase from bacillus stearothermophilus. | | 1982 | 7154941 |
| quaternary structure of the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus studied by a new reversible cross-linking procedure with bis(imidoesters). | the bis(amidine) cross-links formed between protein subunits by treating them with bis(imidoesters) were found to be rapidly broken by exposing the cross-linked proteins to methylamine buffers containing the aprotic solvent acetonitrile. this cleavage step could be introduced between the two dimensions of a diagonal gel electrophoretic separation of cross-linked proteins to facilitate identification of the contributors to a cross-linked species. tests with the tetrameric enzyme glyceraldehyde-3- ... | 1982 | 7171546 |
| affinity labeling of glyceraldehyde-3-phosphate dehydrogenase from sturgeon and bacillus stearothermophilus by 3-chloroacetylpyridine--adenine dinucleotide. kinetic studies. | the inactivation of glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus and sturgeon by 3-chloroacetylpyridine--adenine dinucleotide, which is not active as a coenzyme, obeys pseudo-first-order kinetics. the mechanism of inactivation corresponds to an affinity label. four moles of inactivator are incorporated per mole of tetramer for both enzymes. all the kinetic results strongly support the idea that neither enzyme exhibits negative cooperativity for the non-covalent bindi ... | 1982 | 7173194 |
| quantitative estimates of beta-lactam residues in raw milk around a reference standard: collaborative study. | a collaborative study was conducted to determine the reliability of a bacillus stearothermophilus disc assay method for differentiating various concentrations of penicillin in raw milk. participating laboratories tested 10 different samples (including one negative) in blind duplicate. triplicate standards were alternated with triplicate unknowns around the periphery of each of 5 different plates. zone diameters were measured and the difference in zone size of pairs of adjacent standard and unkno ... | 1982 | 7174583 |
| the structure of the ef-tu . gdp . me2+ complex. | the structure of the mggdp complex at the active site of elongation factor (ef-tu) has been investigated by using phosphorothioate analogs of gdp in the absence and presence of various metal ions, electron paramagnetic resonance (epr) and nuclear magnetic resonance (nmr) measurements. the high stereoselectivity of ef-tu for the diastereomers of guanosine 5'-o-(1-thiodiphosphate) (gdp[alpha s]) is independent of the nature of the metal ion and is caused by the interaction of the protein with the ... | 1982 | 7200884 |
| mass spectrometric sequence studies of a superoxide dismutase from bacillus stearothermophilus. | a partial sequence of the manganese-containing superoxide dismutase from bacillus stearothermophilus has been determined by mass spectrometry. a new fragmentation reaction at n-terminal proline is described and an 'in-chain' fragmentation, also occurring at proline, has been observed. there is some evidence that the latter cleavage may also occur at other residues. some differences from the classically determined sequence are reported, and the validity of these observations is discussed. | 1981 | 7202415 |
| [growth of obligate-thermophilic bacteria on a medium with paraffin]. | the obligate thermophilic bacteria bacillus stearothermophilus (the optimal growth temperature 55-73 degrees c) and thermus ruber (the optimal growth temperature 60 degrees c) were isolated from hot water springs of the kunashir island for the first time in a liquid mineral medium with paraffin. some of the bacteria belonging to bac. stearothermophilus grew at a high rate in a liquid medium with paraffin (strain 16); other strains (12a, 12b) were capable of growing only together with thermus rub ... | 1981 | 7219220 |
| deoxyribonucleic acid modification methylase from bacillus stearothermophilus. | a modification methylase was isolated from bacillus stearothermophilus 1503-4r (bst 1503i) and purified to homogeneity. the enzyme is an acidic protein and composed of a subunit with a molecular weight of 105 000, and only the tetrameric form was detected in solution. the methylase exhibited maximal activity between 54 and 61 degrees c and between ph 8.1 and 9.3. in contrast to bst 1503i endonuclease [catterall, j.f., & welker, n. e. (1977) j. bacteriol. 129, 1110-1120], the methylase is complet ... | 1981 | 7225321 |
| bacillus stearothermophilus spore suspensions: effect of storage conditions and time on viability and moist heat resistance. | | 1981 | 7229838 |
| purification of an nadh-(dichlorophenol-indophenol) oxidoreductase from bacillus stearothermophilus. | an nadh-(dichlorophenol-indophenol) oxidoreductase was purified 104-fold and in 25% overall yield from the thermophilic bacterium bacillus stearothermophilus, strain ph24. after solubilization in 2m-nacl at 70 degrees c, the enzyme was purified by ion-exchange and hydroxyapatite chromatography, followed by affinity chromatography on immobilized cibacron blue 3ga. the purified enzyme had a mol.wt. of 43 000 and had an absorption spectrum characteristic of flavoprotein. the enzyme activity was enh ... | 1980 | 7236205 |
| amino acid activation in crystalline tyrosyl-trna synthetase from bacillus stearothermophilus. | | 1981 | 7265210 |
| functional organization of the large ribosomal subunit of bacillus stearothermophilus. | bacillus stearothermophilus 50 s ribosomal subunits active in polyphenylalanine (polyphe) synthesis were reconstituted from a mixture of purified proteins and rna. proteins were omitted one at a time, and the resulting particles were examined by sucrose gradient sedimentation and assayed for polyphe synthesis, peptidyltransferase activity, and in some cases binding of elongation factor ef-g and gtp, and association with a (20 s . phe-trna . poly(u)) complex. based on their effect on polyphe synt ... | 1981 | 7275969 |
| fidelity of dna replication in vivo. | | 1981 | 7284002 |
| [isolation and properties of two dna-polymerases from bacillus stearothermophilus cells]. | two dna-polymerases (1 and 2) were isolated from the cells of bacillus stearothermophilus and their properties were investigated. the isolation procedure included separation of nucleic acids on deae-cellulose, affinity chromatography on dna-agarose and chromatography on deae-sephadex. dna-polymerase 1 was purified 150-fold; its molecular weight and ph optimum are 135 000 and 7.3, respectively. dna-polymerase 2 was purified 1000-fold; its molecular weight and ph optimum are 95 000 and 9.0, respec ... | 1981 | 7284483 |
| uracil-dna glycosylase from bacillus stearothermophilus. | | 1981 | 7297702 |
| the effect of electron transport on the kinetics of the manganese-containing superoxide dismutase from bacillus stearothermophilus. | | 1981 | 7311574 |
| sequence and secondary structure of the colicin fragment of bacillus stearothermophilus 16s ribosomal rna. | the sequence and the position of post-transcriptionally modified residues of the 3' -terminal end of bacillus stearothermophilus 16s ribosomal rna have been determined from the fragment that is cleaved off by bacteriocin treatment. the fragment contains 52 nucleotides, as compared to the 49 nucleotides of the corresponding fragment from e. coli ribosomes, the additional nucleotides are present in the sequence ucu very next to the 3' -terminus as was published earlier (1). the remainder of the se ... | 1981 | 7312621 |
| [action of gamma and uv irradiation on dna synthesis in permeable bacillus stearothermophilus cells]. | | 1981 | 7313086 |
| bacillus stearothermophilus from saudi arabian soils. | ten thermophilic gram-positive bacteria were isolated from various soils of saudi arabia. the strains are spore-forming rods belonging to the species bacillus stearothermophilus. the cells are motile, strictly aerobic, catalase and oxidase positive. the sporangia appear to be swollen and their position varies from terminal in some to sub-terminal in others. the thermal stability of some enzymes of these bacteria was investigated; extracellular alpha-amylase appears to be very sensitive to ph and ... | 1981 | 7319400 |
| purification and crystallization of protein complex from bacillus stearothermophilus ribosomes. | | 1981 | 7338915 |
| the effect of nucleotide presaturation on the chromatographic behaviour of 6-phosphogluconate dehydrogenase from bacillus stearothermophilus on immobilized triazine dyes. | | 1981 | 7340817 |
| amino acid sequence of a dodecapeptide from the substrate-binding region of the l-lactate dehydrogenase from lactobacillus curvatus, lactobacillus xylosus and bacillus stearothermophilus. | the amino acid sequence of dodecapeptides from the substrate-binding region of 3 bacterial l-lactate dehydrogenases (lactobacillus xylosus, lactobacillus curvatus and bacillus stearothermophilus) were determined. they show a very high homology to the sequences of the corresponding known animal enzymes. there is, however, an essential difference between the sequences of pro- and eucaryotic enzymes: the asn residue in position 166, common to all eucaryotes, is replaced by serine in lactobacilli an ... | 1981 | 7346373 |
| arginyl-transfer ribonucleic acid synthetase of bacillus stearothermophilus. purification and kinetic analysis. | arginyl-trna synthetase from bacillus stearothermophilus (nca 1518) has been purified 880-fold to apparent homogeneity as demonstrated by electrophoresis in the presence of sodium dodecyl sulphate. the molecular weight is 59 000 as confirmed by sephadex g-100 and by sucrose gradient ultracentrifugation. the enzyme is monomeric, no subunits were detected. its cognate trna induces an apparent increase in molecular weight suggesting the dimerisation of the enzyme. nevertheless, it is not obvious th ... | 1980 | 7358046 |
| enolase from bacillus stearothermophilus. physicochemical characterization and reconstitution of the active enzyme from dissociated subunits. | | 1980 | 7372405 |
| the mechanism of action of penicillin. penicillin acylates the active site of bacillus stearothermophilus d-alanine carboxypeptidase. | penicillin kills susceptible bacteria by specifically inhibiting the transpeptidase that catalyzes the final step in cell wall biosynthesis, the cross-linking of peptidoglycan. it was hypothesized (tipper, d., and strominger, j. (1965) proc. natl. acad. sci. u.s.a. 54, 1133-1141) that 1) penicillin is a structural analog of the acyl-d-alanyl-d-alanine terminus of the pentapeptide side chains of nascent peptidoglycan, and that 2) penicillin, by virtue of its highly reactive beta-lactam structure, ... | 1980 | 7372662 |
| phenotypic and genotypic characterization of some thermophilic species of bacillus. | some thermophilic species of bacillus were characterized using biochemical tests, antibiotic sensitivity, bacteriocin and bacteriophage sensitivity, esterase patterns, dna hybridization and % g+c content. the three caldo-active strains of bacilli isolated by heinen (1971) were compared with strains of b. stearothermophilus. eight of the strains examined showed characteristics which enabled them to be placed in the three main taxonomic groups suggested by walker & wolf (1971). two strains showed ... | 1980 | 7391819 |
| superoxide dismutase from bacillus stearothermophilus. complete amino acid sequence of a manganese enzyme. | superoxide dismutase from the thermophilic bacterium bacillus stearothermophilus is dimeric with a molecular weight of 45 487. it contains one atom manganese(iii) per dimeric molecule [brock, c. j., harris, j. i., & sato, s. (1976) j. mol. biol. 107, 175-178]. the subunits are identical, and the following primary structure comprising 203 amino acids has been determined: (formula: see text) the enzyme is identical in 60% of its residues with the escherichia coli b manganese enzyme [steinman, h. m ... | 1980 | 7397109 |
| purification and some properties of 6-phosphoglucose isomerase from bacillus caldotenax. | 6-phosphoglucose isomerase [ec 5.3.1.9] was purified from bacillus caldotenax. the isomerase shared many common properties with the isomerase from b. stearothermophilus, i.e, ph and temperature optima, thermostability, competitive inhibition by 6-phosphogluconate and pi, and amino acid composition. the enzyme activity of the former, however, was lower than that of the latter. the molecular weight of the b. caldotenax isomerase was estimated to be 202,000-204,000 by gel filtration and electrophor ... | 1980 | 7400125 |
| purification and properties of polygalacturonic acid trans-eliminase from bacillus stearothermophilus. | a strain of thermophilic bacteria, bacillus stearothermophilus, with pectolytic activity has been isolated. it produced an endo-polygalacturonic acid trans-eliminase (endo-pate, ec 4.2.2.2) extracellularly when grown at 65 degrees c on a pectic acid medium. the pate was purified 62-fold by the rapid affinity chromatographic method on a sepharose-polygalacturonamide linked matrix. the absorbed pate was eluted from the column with a continuous gradient of 0-10(-3)m ethylenediaminetetraacetic acid ... | 1980 | 7407714 |
| d-glyceraldehyde-3-phosphate dehydrogenase. the purification and characterisation of the enzyme from the thermophiles bacillus stearothermophilus and thermus aquaticus. | 1. d-glyceraldehyde-3-phosphate dehydrogenase from two thermophilic bacteria has been purified by procedures including affinity chromatography on nad+-sepharose. 2. methods for making nad+-free enzyme are also described. 3. both the holo and apo forms of the enzyme from bacillus stearothermophilus have been crystallised. 4. the enzymes are tetrameric and composed of four chemically identical polypeptide chains of molecular weight 36,000. 5. the enzymes are much more stable to heat than their cou ... | 1980 | 7408867 |