| [survey on co-infection with hiv and intestinal parasites in high prevalence areas of hiv/aids, china]. | to understand the co-infection status of hiv and intestinal parasites in a hiv/aids highly endemic area in china. | 2012 | 22799161 |
| co-infection of hiv and intestinal parasites in rural area of china. | intestinal parasite infections (ipis) are among the most significant causes of illness and disease of socially and economically disadvantaged populations in developing countries, including rural areas of the people's republic of china. with the spread of the human immunodeficiency virus (hiv) among rural chinese populations, there is ample scope for co-infections and there have been increasing fears about their effects. however, hardly any relevant epidemiological studies have been carried out i ... | 2012 | 22330320 |
| prevalence of clonorchis sinensis infections along the five major rivers in republic of korea, 2007. | the prevalence of clonorchis sinensis infection was investigated among residents of the five major river basins, that is, hangang, nakdonggang, seomjingang, yeongsangang, and geumgang river basins in korea. | 2010 | 24159439 |
| timps of parasitic helminths - a large-scale analysis of high-throughput sequence datasets. | tissue inhibitors of metalloproteases (timps) are a multifunctional family of proteins that orchestrate extracellular matrix turnover, tissue remodelling and other cellular processes. in parasitic helminths, such as hookworms, timps have been proposed to play key roles in the host-parasite interplay, including invasion of and establishment in the vertebrate animal hosts. currently, knowledge of helminth timps is limited to a small number of studies on canine hookworms, whereas no information is ... | 2013 | 23721526 |
| genome mining offers a new starting point for parasitology research. | parasites including helminthes, protozoa, and medical arthropod vectors are a major cause of global infectious diseases, affecting one-sixth of the world's population, which are responsible for enormous levels of morbidity and mortality important and remain impediments to economic development especially in tropical countries. prevalent drug resistance, lack of highly effective and practical vaccines, as well as specific and sensitive diagnostic markers are proving to be challenging problems in p ... | 2015 | 25563615 |
| selected infectious disease disasters for nursing staff training at egyptian eastern border. | infectious disease disasters are events that involve a biological agent, disease and that result in mass casualties, such as a bioterrorism attack, an emerging outbreak of infectious disease; all disasters pose a risk of infection transmission. but, infectious disease disasters pose the great-risk to illness or death from an infectious disease. this study raised the awareness and improved knowledge by educational program for military nursing staff on selected infectious disease disasters acquire ... | 2014 | 24961011 |
| oral delivery of bacillus subtilis spores expressing cysteine protease of clonorchis sinensis to grass carp (ctenopharyngodon idellus): induces immune responses and has no damage on liver and intestine function. | clonorchis sinensis (c. sinensis) is a fish-borne trematode. human can be infected by ingestion of c. sinensis metacercariae parasitized in grass carp (ctenopharyngodon idella). for induction of effective oral immune responses, spores of bacillus subtilis (b. subtilis) wb600 were utilized as vehicle to delivery cscp (cysteine protease of c. sinensis) cooperated with cotc (b.s-cotc-cp), one of coat proteins, to the gastrointestinal tract. after routine culture of 8-12 h in lb medium, b. subtilis ... | 2017 | 28323213 |
| the immunological characteristics and probiotic function of recombinant bacillus subtilis spore expressing clonorchis sinensis cysteine protease. | clonorchiasis, a food-borne zoonosis, is caused by clonorchis sinensis. the intestinal tract and bile ducts are crucial places for c. sinensis metacercariae to develop into adult worms. the endospore of bacillus subtilis is an ideal oral immunization vehicle for delivery of heterologous antigens to intestine. cysteine protease of c. sinensis (cscp) is an endogenous key component in the excystment of metacercariae and other physiological or pathological processes. | 2016 | 27993173 |
| immune response induced by oral delivery of bacillus subtilis spores expressing enolase of clonorchis sinensis in grass carps (ctenopharyngodon idellus). | clonorchiasis, caused by the consumption of raw or undercooked freshwater fish containing infective metacercariae of clonorchis sinensisis (c.sinensis), remains a common public health problem. new effective prevention strategies are still urgent to control this food-borne infectious disease. the previous studies suggested bacillus subtilis (b. subtilis) spores was an ideal vaccines delivery system, and the c.sinensis enolase (cseno) was a potential vaccine candidate against clonorchiasis. in the ... | 2017 | 27729275 |
| oral delivery of bacillus subtilis spore expressing enolase of clonorchis sinensis in rat model: induce systemic and local mucosal immune responses and has no side effect on liver function. | caused by the consumption of raw or undercooked freshwater fish containing infective metacercariae of clonorchis sinensis, human clonorchiasis remains a major public health problem in china. in previous study, we had expressed enolase from c. sinensis (cseno) on the surface of bacillus subtilis spore and the recombinant spore induced a pronounced protection in terms of reduced worm burden and eggs per gram feces, suggesting b. subtilis spore as an ideal vehicle for antigen delivery by oral treat ... | 2015 | 25877387 |
| systemic and local mucosal immune responses induced by orally delivered bacillus subtilis spore expressing leucine aminopeptidase 2 of clonorchis sinensis. | human clonorchiasis caused by clonorchis sinensis (c. sinensis) has been increasingly prevalent in recent years so that an effective measure is essential and urgent to control the infectious disease. oral delivery of antigens from c. sinensis may be an important approach to effectively induce both systemic and local immune responses to anti-infection of the parasite. in the current study, we used bacillus subtilis (b. subtilis) spores as a delivery vehicle to introduce leucine aminopeptidase 2 o ... | 2014 | 24906994 |
| surface display of clonorchis sinensis enolase on bacillus subtilis spores potentializes an oral vaccine candidate. | clonorchis sinensis (c. sinensis) infections remain the common public health problem in freshwater fish consumption areas. new effective prevention strategies are still the urgent challenges to control this kind of foodborne infectious disease. the biochemical importance and biological relevance render c. sinensis enolase (csenolase) as a potential vaccine candidate. in the present study, we constructed escherichia coli/bacillus subtilis shuttle genetic engineering system and investigated the po ... | 2014 | 24486347 |
| [role of infectious agents in the emergence of malignant tumors]. | according to the data of the international agency for research on cancer (iarc), at least 6 virus species (hpv, ebv, hhv-8/kshv, htlv-1, hbv, hcv), 4 helminthes species (schistosoma haematobium and japonicum, opisthorchis viverrini, clonorchis sinensis) and i bacterium species (helicobacter pylori) have been proved to be capable of causing the development of cancer. the analysis of the data available shows that merkel cell polyomavirus (mcv), herpes simplex virus (hsv), john cunningham polyomavi ... | 2012 | 23163048 |
| multiplex real-time pcr assay targeting eight parasites customized to the korean population: potential use for detection in diarrheal stool samples from gastroenteritis patients. | intestinal parasitic diseases occur worldwide and can cause diarrhea or gastroenteritis; however, their diagnosis is quite difficult, especially in low-endemism countries. we developed a multiplex real-time pcr assay for detection of eight intestinal parasites and prospectively evaluated it for patients with gastroenteritis. the assay targeted cryptosporidium parvum, giardia lamblia, entamoeba histolytica, blastocystis hominis, dientamoeba fragilis, clonorchis sinensis, metagonimus yokogawai, an ... | 2016 | 27861635 |
| trematode metacercariae in freshwater fish from water systems of hantangang and imjingang in republic of korea. | the infection status of freshwater fish with digenetic trematode metacercariae was examined in water systems of hantangang and imjingang (river), the republic of korea. a total of 877 (594 from hantangang and 283 from imjingang) fishes were examined by the artificial digestion methods. clonorchis sinensis metacercariae were detected in 7 (1.2%) fishes (in 3 spp.) from hantangang in cheorwon-gun, gangwon-do, and 40 (14.1%) fishes (in 7 spp.) from munsancheon in paju-si, gyeonggi-do. the average d ... | 2015 | 26174822 |
| prevalence of zoonotic trematode metacercariae in freshwater fish from gangwon-do, korea. | the infection status of zoonotic trematode metacercariae was investigated in a total of 2,293 freshwater fish collected from 11 rivers or streams in 9 administrative regions of gangwon-do, korea for 5 years (2009-2013). all fish were collected by netting methods and examined using the artificial digestion methods. clonorchis sinensis metacercariae were detected in 4 fish species, i.e., pungtungia herzi, squalidus japonicus coreanus, acheilognathus rhombeus, and ladislabia taczanowskii, from only ... | 2014 | 25246719 |
| comparative risk of liver and intestinal fluke infection from either wild-caught or cultured fish in vietnam. | a survey for fish-borne zoonotic trematode (fzt) metacercariae in fish from the northern mountain region of vietnam was conducted from may to august 2014. the major objective was to evaluate the impact of water body types on the prevalence of fzt. a total of 846 fish (31 species) were collected from three water body types, including aquaculture ponds, rivers, and reservoirs. a pepsin digestion method was used for the recovery of metacercariae from fish. overall, 29 of the 31 fish species were in ... | 2016 | 27828767 |
| a cross-sectional study on the potential transmission of the carcinogenic liver fluke opisthorchis viverrini and other fishborne zoonotic trematodes by aquaculture fish. | throughout southeast asia and china, eating raw and or partially cooked cyprinid fish causes liver (hepatobiliary) disease and cancer (cholangiocarcinoma) due to fishborne zoonotic trematodes (fzt), in particular clonorchis sinensis and opisthorchis viverrini. the primary source of transmission is by native fish, but aquaculture fish are also reported to have high infective potential. here, a cross-sectional survey of fzt in fish farms was conducted in an endemic area in khon kaen province, thai ... | 2013 | 23320421 |
| prevalence and intensity of fish-borne zoonotic trematodes in cultured freshwater fish from rural and urban areas of northern vietnam. | consumption of raw freshwater fish produced in both rural farm and urban wastewater ponds is a common practice in vietnam. the present study assessed the risk of fish-borne zoonotic trematode (fzt) infection from fish raised in both these aquaculture systems in northern vietnam. the diversity, prevalence, and infection intensity of fzt metacercariae in 1,500 freshwater fish collected from 6 sites located in rural and urban areas in northern vietnam were investigated. the specific diagnosis of sp ... | 2012 | 22471793 |
| fish sharing as a risk factor for opisthorchis viverrini infection: evidence from two villages in north-eastern thailand. | foodborne trematodiasis (fbt) is a significant global health problem, with the liver flukes opisthorchis viverrini, o. felineus, and clonorchis sinensis contributing to half of the global burden of fbt. north-eastern thailand where o. viverrini is endemic and un-cooked fish dishes remain an integral part of the food culture has the highest reported incidence of opisthorchiasis, including associated cholangiocarcinoma. both food sharing and eating practices are potentially important factors in ft ... | 2017 | 28372560 |
| a new method for estimating the prevalence of clonorchiasis in korea: a proposal to replace arbitrary riverside sampling. | south korea presently uses an arbitrary sampling method to monitor the prevalence of clonorchis sinensis infection in the endemic areas of the country. however, the present method is not standardized and focuses primarily on individuals who reside nearest to the mainstream river. we propose a new sampling method that combines cluster sampling with proportionate quota sampling to ensure that the entire endemic area is accurately represented. we tested the new method in okcheon-gun, south korea, a ... | 2017 | 28353615 |
| recurrent emergence of structural variants of ltr retrotransposon csrn1 evolving novel expression strategy and their selective expansion in a carcinogenic liver fluke, clonorchis sinensis. | autonomous retrotransposons, in which replication and transcription are coupled, encode the essential gag and pol genes as a fusion or separate overlapping form(s) that are expressed in single transcripts regulated by a common upstream promoter. the element-specific expression strategies have driven development of relevant translational recoding mechanisms including ribosomal frameshifting to satisfy the protein stoichiometry critical for the assembly of infectious virus-like particles. retrotra ... | 2017 | 28322871 |
| secreted phospholipase a2 of clonorchis sinensis activates hepatic stellate cells through a pathway involving jnk signalling. | secreted phospholipase a2 (spla2) is a protein secreted by clonorchis sinensis and is a component of excretory and secretory products (csesps). phospholipase a2 is well known for its role in liver fibrosis and inhibition of tumour cells. the jnk signalling pathway is involved in hepatic stellate cells (hscs) activation. blocking jnk activity with sp600125 inhibits hscs activation. in a previous study, the protein csspla2 was expressed in insoluble inclusion bodies. therefore, it's necessary to e ... | 2017 | 28302166 |
| clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin β4-fak/src signaling pathway. | cholangiocarcinoma (cca) is a slow-growing but highly metastatic cancer. its metastatic potential largely explains its high mortality rate. a recognized risk factor for cca development is infection with the liver flukes opisthorchis viverrini and clonorchis sinensis. we previously reported that the excretory-secretory products (esps) of c. sinensis promoted the three-dimensional aggregation and invasion of cca cells. in the present study, a quantitative real-time pcr array of extracellular matri ... | 2017 | 28286026 |
| molecular and structural characterization of the tegumental 20.6-kda protein in clonorchis sinensis as a potential druggable target. | the tegument, representing the membrane-bound outer surface of platyhelminth parasites, plays an important role for the regulation of the host immune response and parasite survival. a comprehensive understanding of tegumental proteins can provide drug candidates for use against helminth-associated diseases, such as clonorchiasis caused by the liver fluke clonorchis sinensis. however, little is known regarding the physicochemical properties of c. sinensis teguments. in this study, a novel 20.6-kd ... | 2017 | 28273846 |
| mitochondrial genomic comparison of clonorchis sinensis from south korea with other isolates of this species. | clonorchiasis is a neglected tropical disease that affects >35 million people mainly in china, vietnam, south korea and some parts of russia. the disease-causing agent, clonorchis sinensis, is a liver fluke of humans and other piscivorous animals, and has a complex aquatic life cycle involving snails and fish intermediate hosts. chronic infection in humans causes liver disease and associated complications including malignant bile duct cancer. central to control and to understanding the epidemiol ... | 2017 | 28254628 |
| risk mapping of clonorchiasis in the people's republic of china: a systematic review and bayesian geostatistical analysis. | clonorchiasis, one of the most important food-borne trematodiases, affects more than 12 million people in the people's republic of china (p.r. china). spatially explicit risk estimates of clonorchis sinensis infection are needed in order to target control interventions. | 2017 | 28253272 |
| heat shock proteins 70 and 90 from clonorchis sinensis induce th1 response and stimulate antibody production. | heat shock proteins (hsps) are found in all prokaryotes and most compartments of eukaryotic cells. members of the hsp family mediate immune responses to tissue damage or cellular stress. however, little is known about the immune response induced by the oriental liver fluke, clonorchis sinensis, even though this organism is carcinogenic to humans. we address this issue in the present study in mouse bone marrow dendritic cells (mbmdcs), using recombinant hsp70 and 90 from c. sinensis (rcshsp70 and ... | 2017 | 28249599 |
| a comparative study of mud-like and coralliform calcium carbonate gallbladder stones. | to gain insight to underlying mechanism of the formation of calcium carbonate (caco3 ) gallbladder stones, we did comparative study of stones with mud appearance and those with coralliform appearance. a total of 93 gallbladder stones with mud appearance and 50 stones with coralliform appearance were analyzed. the appearance, color, texture, and the detection of clonorchis sinensis eggs by microscopic examination were compared between the two groups. then, the material compositions of stones were ... | 2017 | 28245082 |
| expression of clonorchis sinensis giiispla2 protein in baculovirus-infected insect cells and its overexpression facilitating epithelial-mesenchymal transition in huh7 cells via akt pathway. | although prior studies confirmed that group iii secretory phospholipase a2 of clonorchis sinensis (csgiiispla2) had stimulating effect on liver fibrosis by binding to lx-2 cells, large-scale expression of recombinant protein and its function in the progression of hepatoma are worth exploring. because of high productivity and low lipopolysaccharides (lps) in the sf9-baculovirus expression system, we firstly used this system to express the coding region of csgiiispla2. the molecular weight of reco ... | 2017 | 28220242 |
| increased hepatic th2 and treg subsets are associated with biliary fibrosis in different strains of mice caused by clonorchis sinensis. | previous studies showed that cd4+t cells responses might be involved in the process of biliary fibrosis. however, the underlying mechanism resulting in biliary fibrosis caused by clonorchis sinensis remains not yet fully elucidated. the objectives of the present study were to investigate the different profiles of hepatic cd4+t cell subsets (th1, th2, th17 and treg cells) and their possible roles in the biliary fibrosis of different strains of mice (c57bl/6, balb/c and fvb mice) induced by c. sin ... | 2017 | 28151995 |
| cell-free translational screening of an expression sequence tag library of clonorchis sinensis for novel antigen discovery. | the rapidly evolving cloning and sequencing technologies have enabled understanding of genomic structure of parasite genomes, opening up new ways of combatting parasite-related diseases. to make the most of the exponentially accumulating genomic data, however, it is crucial to analyze the proteins encoded by these genomic sequences. in this study, we adopted an engineered cell-free protein synthesis system for large-scale expression screening of an expression sequence tag (est) library of clonor ... | 2017 | 28127897 |
| [control of opisthorchis viverrini infection for cholangiocarcinoma prevention]. | the international agency for research on cancer (iarc) has classified two liver flukes as carcinogenic to humans (group 1): opisthorchis viverrini in 1994 and clonorchis sinensis in 2009. this review is focused on o. viverrini, the most studied of these two trematodes, which infects nearly 10 million people in southeast asia. the life cycle involves two intermediate hosts living in fresh water: a snail of the genus bithynia and a ciprinid fish. the definitive hosts (human, cat, dog) become infec ... | 2017 | 28105582 |
| molecular and structural characteristics of multidrug resistance-associated protein 7 in chinese liver fluke clonorchis sinensis. | multidrug resistance-associated protein 7 (mrp7, abcc10) is a c subfamily member of the atp-binding cassette (abc) superfamily. mrp7 is a lipophilic anion transporter that pumps endogenous and xenobiotic substrates from the cytoplasm to the extracellular milieu. here, we cloned and characterized csmrp7 as a novel abc transporter from the chinese liver fluke, clonorchis sinensis. full-length cdna of csmrp7 was 5174 nt, encoded 1636 amino acids (aa), and harbored a 147-bp 5'-untranslated region (5 ... | 2017 | 28058535 |
| risk factors for clonorchis sinensis infection transmission in humans in northern vietnam: a descriptive and social network analysis study. | clonorchis sinensis is major fish-borne trematode, endemic in north vietnam. risk factors described so far include individual eating behaviors and environmental factors. here, additional to conventional risk factors, we report on socially influenced liver fluke transmission in endemic communities. a cross-sectional study on risk factors and fish sharing networks was conducted in 4 villages of gia thinh commune, ninh binh province. a total of 510 residents in 272 households were recruited for ris ... | 2017 | 27939296 |
| clonorchis sinensis excretory-secretory products regulate migration and invasion in cholangiocarcinoma cells via extracellular signal-regulated kinase 1/2/nuclear factor-κb-dependent matrix metalloproteinase-9 expression. | matrix metalloproteinase-9 plays an important role in the invasion and metastasis of various types of cancer cells. we have previously reported that excretory-secretory products from clonorchis sinensis increases matrix metalloproteinase-9 expression. however, the regulatory mechanisms through which matrix metalloproteinase-9 expression affects cholangiocarcinoma development remain unclear. in the current study, we examined the potential role of excretory-secretory products in regulating the mig ... | 2017 | 27919591 |
| dysregulation of hepatic microrna expression profiles with clonorchis sinensis infection. | clonorchiasis remains an important zoonotic parasitic disease worldwide. the molecular mechanisms of host-parasite interaction are not fully understood. non-coding micrornas (mirnas) are considered to be key regulators in parasitic diseases. the regulation of mirnas and host micro-environment may be involved in clonorchiasis, and require further investigation. | 2016 | 27899092 |
| local immune response to primary infection and re-infection by clonorchis sinensis in fvb mice. | although clonorchis sinensis lives in the bile duct, few studies have investigated the local immune response in the liver and bile duct. to investigate the local immune response to c. sinensis, we investigated the activation and recruitment of various immune cells and cytokine levels in the liver and bile duct lymph nodes (bln) in fvb mice after primary infection and re-infection. male 4-week-old fvb mice were divided into 6 experimental groups: uninfected controls, primary infection lasting 1we ... | 2016 | 27856336 |
| toll-like receptor-mediated free radical generation in clonorchis sinensis excretory-secretory product-treated cholangiocarcinoma cells. | clonorchiasis, caused by direct contact with clonorchis sinensis worms and their excretory-secretory products (esps), is associated with chronic inflammation, malignant changes in bile ducts, and even cholangiocarcinogenesis. our previous report revealed that intracellular free radicals enzymatically generated by c. sinensis esps cause nf-κb-mediated inflammation in human cholangiocarcinoma cells (hucct1). therefore, the present study was conducted to examine the role of upstream toll-like recep ... | 2016 | 27853127 |
| ancient human parasites in ethnic chinese populations. | whilst archaeological evidence for many aspects of life in ancient china is well studied, there has been much less interest in ancient infectious diseases, such as intestinal parasites in past chinese populations. here, we bring together evidence from mummies, ancient latrines, and pelvic soil from burials, dating from the neolithic period to the qing dynasty, in order to better understand the health of the past inhabitants of china and the diseases endemic in the region. seven species of intest ... | 2016 | 27853113 |
| peroxiredoxin 6 expression is inversely correlated with nuclear factor-κb activation during clonorchis sinensis infestation. | clonorchis sinensis is a carcinogenic human liver fluke. its infection promotes persistent oxidative stress and chronic inflammation environments in the bile duct and surrounding liver tissues owing to direct contact with worms and their excretory-secretory products (esps), provoking epithelial hyperplasia, periductal fibrosis, and cholangiocarcinogenesis. we examined the reciprocal regulation of two esp-induced redox-active proteins, nf-κb and peroxiredoxin 6 (prdx6), during c. sinensis infecti ... | 2016 | 27554973 |
| liver fluke infection and cholangiocarcinoma: a review. | parasites are significant groups for carcinogenesis among which liver flukes, including opisthorchis viverrini and clonorchis sinensis, are typical representatives causing cholangiocarcinoma (cca), the second most common primary hepatic malignancy with dismal prognosis. o. viverrini is prevalent in southeast asia, infecting 10 million people while c. sinensis has a wider distribution in east asia and several southeast asian countries, affecting more than 35 million people's health. these two wor ... | 2017 | 27718017 |
| clonorchis sinensis, an oriental liver fluke, as a human biological agent of cholangiocarcinoma: a brief review. | parasitic diseases remain an unarguable public health problem worldwide. liver fluke clonorchis sinensis is a high risk pathogenic parasitic helminth which is endemic predominantly in asian countries, including korea, china, taiwan, vietnam, and the far eastern parts of russia, and is still actively transmitted. according to the earlier 8th national survey on the prevalence of intestinal parasitic infections in 2012, c. sinensis was revealed as the parasite with highest prevalence of 1.86% in ge ... | 2016 | 27418285 |
| painless jaundice caused by clonorchis sinensis infection: a case report. | a man with only yellowing of the skin and eye sclera was diagnosed with clonorchiasis, which rarely manifested jaundice as the initial symptom. however, because of a lack of evidence for a diagnostic gold standard, the time until definitive diagnosis was more than a week. the diagnostic process relied on inquiring about the patient's history, including the place of residence, dietary habits, and symptoms, as well as on serological findings, an imaging examination, and pathological findings. mrcp ... | 2016 | 27417088 |
| c3h/he mice as an incompatible cholangiocarcinoma model by clonorchis sinensis, dicyclanil and n-nitrosodimethylamine. | clonorchis sinensis is a group-i bio-carcinogen, associated with cholangiocarcinoma (cca). the hamster is the only experimental model of c. sinensis-mediated cca, but we oblige another animal model. the present study intended to develop a c. sinensis (cs) mediated cca model using c3h/he mice, co-stimulated with n-nitrosodimethyl-amine (ndma) and dicyclanil (dc). the mice were divided into 8 groups with different combinations of cs, ndma, and dc. six months later the mice were sacrificed and subj ... | 2016 | 27417082 |
| chromosome morphometry in opisthorchiid species (platyhelminthes, trematoda). | few existing studies have dealt with cytogenetics in trematodes, largely due to the attendant technical difficulty of chromosome preparation. we performed a comparative analysis of chromosomes in five opistorchiid species, including opisthorchis felineus rivolta, 1884, opisthorchis viverrini poirier, 1886, clonorchis sinensis cobbold, 1875, metorchis xanthosomus creplin 1846, and metorchis bilis (braun, 1790) odening, 1962. for some of these species, no detailed morphometric description of their ... | 2016 | 27404484 |
| current status and perspectives of clonorchis sinensis and clonorchiasis: epidemiology, pathogenesis, omics, prevention and control. | clonorchiasis, caused by clonorchis sinensis (c. sinensis), is an important food-borne parasitic disease and one of the most common zoonoses. currently, it is estimated that more than 200 million people are at risk of c. sinensis infection, and over 15 million are infected worldwide. c. sinensis infection is closely related to cholangiocarcinoma (cca), fibrosis and other human hepatobiliary diseases; thus, clonorchiasis is a serious public health problem in endemic areas. this article reviews th ... | 2016 | 27384714 |
| clonorchis sinensis co-infection could affect the disease state and treatment response of hbv patients. | clonorchis sinensis (c. sinensis) is considered to be an important parasitic zoonosis because it infects approximately 35 million people, while approximately 15 million were distributed in china. hepatitis b virus (hbv) infection is a major public health issue. two types of pathogens have the potential to cause human liver disease and eventually hepatocellular carcinoma. concurrent infection with hbv and c. sinensis is often observed in some areas where c. sinensis is endemic. however, whether c ... | 2016 | 27348302 |
| characterization and identification of differentially expressed micrornas during the process of the peribiliary fibrosis induced by clonorchis sinensis. | clonorchis sinensis (c. sinensis) infection can lead to biliary fibrosis. micrornas (mirnas) play important roles in regulation of genes expression in the liver diseases. however, the differential expression of mirnas that probably regulates the portal fibrogenesis caused by c. sinensis has not yet been investigated. hepatic mirnas expression profiles from c. sinensis-infected mice at different time-points were analyzed by mirna microarray and validated by quantitative real-time pcr (qrt-pcr). 3 ... | 2016 | 27267304 |
| characterization and localization of opisthorchis viverrini fructose-1,6-bisphosphate aldolase. | opisthorchis viverrini (ov) infection is a long-time public health problem in thailand that can lead to bile duct cancer, cholangiocarcinoma (cca). characterization of the ov proteins at a molecular level will increase our knowledge of host-parasite interaction that can be applied to new drug, vaccine, or immunodiagnostic development. in this study, an important enzyme in the ov glycolytic pathway, fructose-1,6-bisphosphate aldolase (fbpa), that had been obtained from a previous study was charac ... | 2016 | 27265876 |
| prevalence of clonorchis sinensis infection among residents along 5 major rivers in the republic of korea. | clonorchis sinensis is currently the most important parasite affecting public health problems in the republic of korea. we investigated the prevalence of c. sinensis infection among residents living along 5 major rivers in korea. a total of 42,562 individual stool samples were collected from 37 localities and examined using the formalin-ether sedimentation technique. helminth eggs were detected in 4,052 (9.5%) residents and 3,586 (8.4%) were infected with c. sinensis. the egg positive rate of c. ... | 2016 | 27180582 |
| the importance of wild fish in the epidemiology of clonorchis sinensis in vietnam. | preliminary findings of a high prevalence of clonorchis sinensis in wild-caught fish in a north vietnam reservoir (thac ba reservoir, yen bai province) prompted a longitudinal epidemiological study of fish infections. monthly collections of fish from september 2014 to august 2015 were processed for recovery of metacercariae; 1219 fish, representing 22 species, were examined. seven species were infected with c. sinensis metacercariae. four species, toxabramis houdemeri, hemiculter leucisculus, cu ... | 2016 | 27160330 |
| characterization and functional analysis of fatty acid binding protein from the carcinogenic liver fluke, opisthorchis viverrini. | in the present study, the cdna encoding fabp (ov-fabp) was isolated from the adult stage of opisthorchis viverrini and characterized. the ov-fabp protein sequence (107 amino acids) was predicted to have a molecular mass of 12.26kda and an isoelectric point (pi) of 6.82. this sequence had a high identity and similarity to cs-fabp of the related opisthorchid clonorchis sinensis. multiple sequence alignment with fabps from other parasitic flatworms and mammals showed a number of conserved amino aci ... | 2016 | 27143603 |
| screening and identification of dna aptamers toward schistosoma japonicum eggs via selex. | schistosomiasis is a major parasitic disease caused by blood flukes of the genus schistosoma. several million people all over the world are estimated to suffer from severe morbidity as a consequence of schistosomiasis. the worm's eggs, which cause the symptoms of schistosomiasis, are generally used to diagnose the disease. in this study, we employed egg-based systematic evolution of ligands by exponential enrichment (egg-selex) and identified a panel of ssdna aptamers specifically binding to egg ... | 2016 | 27121794 |
| [epidemiological investigation of second intermediate hosts of clonorchis sinensis in pizhou and xinyi cities of jiangsu province]. | to understand the endemic situation of clonorchis sinensis in its second intermediate hosts in pizhou and xinyi cities of jiangsu province, so as to provide the evidence for the further control and treatment. | 2015 | 27097495 |
| [discussion on relationship between hepatobiliary pathological changes under b-ultrasound and clonorchis sinensis infection]. | to explore the relationship between the hepatobiliary pathological changes under b-ultrasound examinations and clonorchis sinensis infection, so as to provide the evidence for further prevention and control. | 2015 | 27097486 |
| [survey of epidemic status of principal human parasites in jiangxi province in 2014]. | to understand and analyze the epidemic status of principal human parasites in jiangxi province, so as to provide the evidence for formulating the 13rd five-year project of parasites control. | 2015 | 27097476 |
| interleukin-13 is involved in the formation of liver fibrosis in clonorchis sinensis-infected mice. | clonorchiasis is a chronic infection disease often accompanied by formation of liver fibrosis. previous study has identified that clonorchis sinensis (c. sinensis, cs) infection and csrnaset2 (a member of csesps) immunization can drive th2 immune response. il-13, a multifunctional th2 cytokine, has been widely confirmed to be profibrotic mediator. we want to determine whether il-13 is involved in the generation of liver fibrosis during c. sinensis infection. a part of mice were infected with c. ... | 2016 | 26993324 |
| expression and potential roles of il-33/st2 in the immune regulation during clonorchis sinensis infection. | during clonorchiasis, immune responses of hosts are responsible for the removal of the worms and also are involved in the progress of the pathological damage caused by clonorchis sinensis. interleukin-33 (il-33), a recently described cytokine signaling through the st2 receptor, has emerged as a potent inducer to bile duct proliferation and fibrosis; however, little is known of this signaling in the pathogen-caused periductal inflammation and fibrosis. in the present study, using immunohistochemi ... | 2016 | 26944417 |
| carcinogenesis associated with parasites other than schistosoma, opisthorchis and clonorchis: a systematic review. | only three helminths (schistosoma haematobium, opisthorchis viverrini and clonorchis sinensis) are directly associated with carcinogenesis in humans whereas the role of other parasites in cancer remains unclear. this study aimed to perform a systematic review to identify recent insights in the role of other parasite infections in carcinogenesis. we conducted systematic searches of medline and embase on july 2015. our primary outcome was the association between parasitic infections and carcinogen ... | 2016 | 26840624 |
| helicobacter species are possible risk factors of cholangiocarcinoma. | several infectious agents are considered to be causes of cancer in human, mainly hepatitis b and c viruses, high-risk human pailloma viruses, helicobacter pylori, clonorchis sinensis, and opisthorchis viverrini. here we described the evident research and the association between helicobacter spp. and biliary tract cancer particularly cholangiocarcinoma (cca). global epidemiological studies have suggested that helicobacter spp. are possible risk factors for biliary tract diseases. molecular studie ... | 2016 | 26838240 |
| hemozoin is a product of heme detoxification in the gut of the most medically important species of the family opisthorchiidae. | many species of trematodes such as schistosoma spp., fasciola hepatica and echinostoma trivolvis are blood-feeding parasites. nevertheless, there is no consensus on the feeding habits of the family opisthorchiidae (opisthorchis felineus, opisthorchis viverrini and clonorchis sinensis). previously, histological studies of o. felineus and c. sinensis revealed some dark stained material in their gut lumen. in this study we conducted a comprehensive analysis of the gut contents of three members of t ... | 2016 | 26812025 |
| the complete mitochondrial genome of metorchis orientalis (trematoda: opisthorchiidae): comparison with other closely related species and phylogenetic implications. | metorchis orientalis (trematoda: opisthorchiidae) is an important trematode infecting many animals and humans, causing metorchiasis. in the present study, the complete mitochondrial (mt) genome of m. orientalis was sequenced. the complete mt genome of m. orientalis is 13,834 bp circular dna molecule and contains 12 protein-coding genes, 22 transfer rna genes, and two ribosomal rna genes. the gene content and arrangement of m. orientalis is the same as those of opisthorchiidae trematodes (opistho ... | 2016 | 26805437 |
| pathological lesions and inducible nitric oxide synthase expressions in the liver of mice experimentally infected with clonorchis sinensis. | the nitric oxide (no) formation and intrinsic nitrosation may be involved in the possible mechanisms of liver fluke-associated carcinogenesis. we still do not know much about the responses of inducible no synthase (inos) induced by clonorchis sinensis infection. this study was conducted to explore the pathological lesions and inos expressions in the liver of mice with different infection intensity levels of c. sinensis. extensive periductal inflammatory cell infiltration, bile duct hyperplasia, ... | 2015 | 26797449 |
| phylogenetic characterization of clonorchis sinensis proteins homologous to the sigma-class glutathione transferase and their differential expression profiles. | glutathione transferase (gst) is one of the major antioxidant proteins with diverse supplemental activities including peroxidase, isomerase, and thiol transferase. gsts are classified into multiple classes on the basis of their primary structures and substrate/inhibitor specificity. however, the evolutionary routes and physiological environments specific to each of the closely related bioactive enzymes remain elusive. the sigma-like gsts exhibit amino acid conservation patterns similar to the pr ... | 2016 | 26792248 |
| collection methods of trematode eggs using experimental animal models. | although observing the eggs of human parasitic helminth is essential for medical education in parasitology, opportunities for collection of the eggs are limited. collection of the eggs using experimental animal models is needed for a sustainable supply. the metacercariae of three trematode species, paragonimus westermani, clonorchis sinensis and metagonimus yokogawai, were collected from the second intermediate hosts: freshwater crabs and fishes, which were obtained using online shopping in japa ... | 2016 | 26792074 |
| antibodies against clonorchis sinensis ldh could cross-react with ldhb localizing on the plasma membrane of human hepatocarcinoma cell smmc-7721 and induce apoptosis. | lactate dehydrogenase (ldh) is a terminal enzyme in anaerobic glycolytic pathway. it widely exists in various organisms and is in charge of converting the glycolysis product pyruvic acid to lactic acid. most parasites, including clonorchis sinensis, predominantly depend on glycolysis to provide energy. bioinformatic analysis predicts that the ldhs from many species have more than one transmembrane region, suggesting that it may be a membrane protein. c. sinensis ldh (csldh) has been confirmed as ... | 2016 | 26769711 |
| the liver fluke opisthorchis felineus: biology, epidemiology and carcinogenic potential. | the liver fluke opisthorchis felineus is a member of the triad of epidemiologically important liver fluke species belonging to the family opisthorchiidae and the major agent causing opisthorchiasis over a vast territory, covering russia, kazakhstan and several european countries. the similarity between the diseases caused by o. felineus and other liver flukes, o. viverrini and clonorchis sinensis, in clinical manifestations and course suggests that the scenarios of their development and, possibl ... | 2016 | 26740360 |
| current status of clonorchis sinensis and clonorchiasis in china. | the oriental liver fluke, clonorchis sinensis, a pathogen causing clonorchiasis, is of major socio-economic importance in east asia, including china, korea and vietnam. this parasite is now recognized as a biocarcinogen strongly linked to cholangiocarcinoma in humans. here, we describe the status of clonorchiasis in china, where it has been estimated that more than 15 million patients are affected. this paper also summarizes the major advances in the field of clonorchiasis research during last d ... | 2016 | 26740359 |
| clonorchis sinensis and opisthorchis spp. in vietnam: current status and prospects. | clonorchis sinensis and opisthorchis viverrini are clinically important small liver flukes because of their known association with development of cholangiocarcinoma. in vietnam, high prevalence of c. sinensis infection in humans was previously reported in northern provinces, and o. viverrini infection has been detected in several central provinces. however, diagnosis of c. sinensis and o. viverrini infections in the past was merely based on faecal egg examination. this method alone can lead to m ... | 2016 | 26740358 |
| oxidative stress-mediated mouse liver lesions caused by clonorchis sinensis infection. | clonorchis sinensis is a high-risk pathogenic helminth that strongly provokes inflammation, epithelial hyperplasia, periductal fibrosis, and even cholangiocarcinoma in chronically infected individuals. chronic inflammation is associated with an increased risk of various cancers due to the disruption of redox homeostasis. accordingly, the present study was conducted to examine the time course relationship between histopathological changes and the appearance of oxidative stress markers, including ... | 2016 | 26718397 |
| identification and characterization of clonorchis sinensis cathepsin b proteases in the pathogenesis of clonorchiasis. | human clonorchiasis is a prevailing food-borne disease caused by clonorchis sinensis infection. functional characterizations of key molecules from c. sinensis could facilitate the intervention of c. sinensis associated diseases. | 2015 | 26691339 |
| clonorchis sinensis ova in bile juice cytology from a patient with severe hyperbilirubinemia and portal vein thrombosis. | infection with the trematode clonorchis sinensis is the most common human fluke infection in east asian populations. although this infection is associated with obstructive jaundice or choledocholithiasis, portal vein thrombosis has not been reported. here, we report the first case of a 60-year-old man who had both c. sinensis infection and portal vein thrombosis with severe hyperbilirubinemia (75.4 mg/dl). he initially presented with abdominal pain and jaundice. computed tomography revealed gall ... | 2016 | 26663478 |
| the role of rice fields, fish ponds and water canals for transmission of fish-borne zoonotic trematodes in aquaculture ponds in nam dinh province, vietnam. | fish-borne zoonotic trematodes (fzt), such as clonorchis sinensis, opistorchis viverini (opisthorchiidae) and intestinal trematodes of the family heterophyidae, constitute a public health hazard in vietnam and infections with these trematodes has been linked to consumption of raw or undercooked fish from aquaculture. the fzt transmission pathways, however, are more complicated than just the presence of intermediate snail hosts in aquaculture ponds as ponds may exchange water with surrounding hab ... | 2015 | 26642755 |
| functional analysis of the unique cytochrome p450 of the liver fluke opisthorchis felineus. | the basic metabolic cytochrome p450 (cyp) system is essential for biotransformation of sterols and xenobiotics including drugs, for synthesis and degradation of signaling molecules in all living organisms. most eukaryotes including free-living flatworms have numerous paralogues of the cyp gene encoding heme monooxygenases with specific substrate range. notably, by contrast, the parasitic flatworms have only one cyp gene. the role of this enzyme in the physiology and biochemistry of helminths is ... | 2015 | 26625139 |
| why does infection with some helminths cause cancer? | infections with opisthorchis viverrini, clonorchis sinensis and schistosoma haematobium are classified as group 1 biological carcinogens: definitive causes of cancer. these worms are metazoan eukaryotes, unlike the other group 1 carcinogens including human papilloma virus, hepatitis c virus, and helicobacter pylori. by contrast, infections with phylogenetic relatives of these helminths, also trematodes of the phylum platyhelminthes and major human pathogens, are not carcinogenic. these inconsist ... | 2015 | 26618199 |
| the dynamics of treg/th17 and the imbalance of treg/th17 in clonorchis sinensis-infected mice. | clonorchiasis, caused by the liver fluke clonorchis sinensis, is a chronic parasitic infection regulated by t cell subsets. an imbalance of cd4+cd25+ foxp3+regulatory t (treg) and interleukin (il)-17-secreting t cells (th17) may control inflammation and play an important role in the pathogenesis of immune evasion. in the present study, we assessed the dynamics of treg/th17 and determined whether the treg/th17 ratio is altered in c. sinensis-infected mice. the results showed that the percentages ... | 2015 | 26599407 |
| cell-free expression and in situ immobilization of parasite proteins from clonorchis sinensis for rapid identification of antigenic candidates. | progress towards genetic sequencing of human parasites has provided the groundwork for a post-genomic approach to develop novel antigens for the diagnosis and treatment of parasite infections. to fully utilize the genomic data, however, high-throughput methodologies are required for functional analysis of the proteins encoded in the genomic sequences. in this study, we investigated cell-free expression and in situ immobilization of parasite proteins as a novel platform for the discovery of antig ... | 2015 | 26599101 |
| [excystation time and location of metacercariae of clonorchis sinensis in mice]. | metacercariae of clonorchis sinensis (20 per mouse) were given orally to kunming mice. the mice were sacrificed at 0.5, 1, 2, 4, 8, 16, 32, and 64 h after infection. the results showed that the excystation of the metacercariae occurred in the stomach and small intestine, mainly in the duodenum. excystation occurred mainly at 2, 4, 8, and 16 h post-infection. juveniles were found in hepatic duct on 0.5 h post-infection. the number of juveniles increased with the extension of infection time, which ... | 2015 | 26541046 |
| [establishment of duplex pcr for identifying metagonimus yokogawai and haplorchis taichui]. | to develop a duplex pcr method for identifying metagonimus yokogawai and haplorchis taichui. | 2015 | 26541032 |
| [gallbladder stone types in patients with cholelithiasis and clonorchis sinensis infection in the endemic area of clonorchiasis]. | to investigate the relationship between clonorchis sinensis infection and the gallbladder stone type in patients with cholelithiasis in the endemic area of clonorchiasis. | 2015 | 26541030 |
| expression of toll-like receptor (tlr) 2 and tlr4 in the livers of mice infected by clonorchis sinensis. | clonorchis sinensis is one of the most important foodborne pathogens in humans, and can cause biliary diseases such as gallstones, cholecystitis, cholangitis, and cholangiocarcinoma. toll-like receptors (tlrs) as sensors are crucial to initiating both innate and adaptive immune defenses against pathogens. however, little is known about the hepatic expression of tlrs of hosts induced by c. sinensis infection. | 2015 | 26517491 |
| clonorchis sinensis excretory/secretory products promote the secretion of tnf-alpha in the mouse intrahepatic biliary epithelial cells via toll-like receptor 4. | toll-like receptor 4 (tlr4), as one of the most important pathogen pattern recognitions (pprs) plays a central role in elicitation of innate immunity and mediation of adaptive responses against foreign antigens. however, little is known of the roles of tlr4 in the immune responses of biliary epithelial cells (becs) induced by clonorchis sinensis, a parasite of significance in human health. | 2015 | 26497121 |
| clonorchis sinensis lysophospholipase inhibits tgf-β1-induced expression of pro-fibrogenic genes through attenuating the activations of smad3, jnk2, and erk1/2 in hepatic stellate cell line lx-2. | liver fibrosis is a wound healing response associated with chronic liver injury. hepatic stellate cells (hscs) activation is a key event in the development of liver fibrosis. since helminths have the ability to live for decades in the host by establishing an adaptive relationship in the interplay with its hosts, we hypothesize that whether clonochis sinensis lysophospholipasea (cslysopla), a component of excretory/secretory proteins, can attenuate the fibrogenic response by inhibiting activation ... | 2016 | 26486942 |
| molecular cloning and functional characterization of a glucose transporter (csglut) in clonorchis sinensis. | a complementary dna (cdna) encoding a glucose transporter of clonorchis sinensis (csglut) was isolated from the adult c. sinensis cdna library. the open reading frame of csglut cdna consists of 1653 base pairs that encode a 550-amino acid residue protein. hydropathy analysis suggested that csglut possess 12 putative membrane-spanning domains. the northern blot analysis result using poly(a)(+)rna showed a strong band at ~2.1 kb for csglut. when expressed in xenopus oocytes, csglut mediated the tr ... | 2016 | 26450594 |
| liver fluke-infested graft used for living-donor liver transplantation: case report and review of the literature. | clonorchiasis is a cholangiopathy caused by foodborne trematode parasites, also known as liver flukes. clonorchiasis is endemic in a wide geographical area extending from eastern europe to southeast asia. infested hosts may remain asymptomatic for decades and consequently their liver can become available as a graft. to date, 20 liver transplantations with liver fluke-infested grafts have been reported in the literature. all of them occurred in asian countries. we, here, report the first case to ... | 2015 | 26437122 |
| investigation on oxidative stress of nitric oxide synthase interacting protein from clonorchis sinensis. | numerous evidences indicate that excretory-secretory products (esps) from liver flukes trigger the generation of free radicals that are associated with the initial pathophysiological responses in host cells. in this study, we first constructed a clonorchis sinensis (c. sinensis, cs)-infected balb/c mouse model and examined relative results respectively at 3, 5, 7, and 9 weeks postinfection (p.i.). quantitative reverse transcription (rt)-pcr indicated that the transcriptional level of both endoth ... | 2016 | 26391171 |
| whole transcriptome profiling of adult and infective stages of the trematode opisthorchis felineus. | opisthorchis felineus, the trematode belonging to the family opisthorchiidae, is a causative agent of the infection called opisthorchiasis or liver fluke infection. being a close relative of opisthorchis viverrini and clonorchis sinensis (oriental liver flukes) it is encountered in northern eurasia, especially in russia, kazakhstan, belarus, ukraine, and baltic countries. whole genome data for oriental liver flukes revealed their adaptations for life in the bile duct but our knowledge of o. feli ... | 2016 | 26363139 |
| involvement of psmd10, cdk4, and tumor suppressors in development of intrahepatic cholangiocarcinoma of syrian golden hamsters induced by clonorchis sinensis and n-nitrosodimethylamine. | clonorchis sinensis is a group-i bio-carcinogen for cholangiocarcinoma (cca). although the epidemiological evidence links clonorchiasis and cca, the underlying molecular mechanism involved in this process is poorly understood. in the present study, we investigated expression of oncogenes and tumor suppressors, including psmd10, cdk4, p53 and rb in c. sinensis induced hamster cca model. | 2015 | 26313366 |
| clonorchiasis. | on aug 21, 1875, james mcconnell published in the lancet his findings from a post-mortem examination of a 20-year-old chinese man--undertaken at the medical college hospital in calcutta, india--in whom he found clonorchis sinensis in the bile ducts. now, exactly 140 years later, we have a sound understanding of the lifecycle of this liver fluke, including key clinical, diagnostic, and epidemiological features. developments in the so-called -omics sciences have not only advanced our knowledge of ... | 2016 | 26299184 |
| [establishment and diagnostic performance of biotin-avidin complex enzyme linked immunosorbent assay of detecting specific igg4 of clonorchiasis]. | to explore the performance of the biotin-avidin complex enzyme linked immunosorbent assay of detecting specific igg4 for the diagnosis of clonorchiasis. | 2015 | 26263776 |
| [progress on the relationship between clonorchis sinensis infection and cholangiocarcinoma]. | currently, 12.49 million people are infected with clonorchis sinensis in china. the incidence of bile duct carcinoma increased for recent years. more than a century ago, some scholars have put forward the idea about the relations between c. sinensis infection and cholangiocarcinoma, and committed to research the mechanism. however, the intrinsic mechanisms involved in these processes remain obscure. it is therefore important to pay more attention to the further investigation of the relevance bet ... | 2015 | 26245128 |
| [effect of excretory-secretory products of clonorchis sinensis on nitric oxide production and nf-κb activation in raw264.7 mouse macrophages]. | to study the role of excretory-secretory products (esps) from clonorchis sinensis in the production of nitric oxide (no) and the activation of nuclear transcription factor kappa b (nf-κb) in the macrophages of raw264.7 mouse. | 2015 | 26245117 |
| [cloning, expression and immunodiagnostic evaluation of the fasciola gigantica thioredoxin peroxidase]. | to immunoscreen the gene encoding thioredoxin peroxidase (tpx) from a cdna library made from adult fasciola gigantica worms, clone and express the gene, and evaluate the immunodiagnostic value of tpx recombinant protein. | 2015 | 26245114 |
| [pathogens of human trematodiases in the primorye territory]. | the primorye territory of russia is part of a natural habitat for pathogens of four human trematodiases: metagonimiasis (caused by the two worms metagonimus yokogawai and m. katsuradai in the region), nanophyetiasis (nanophyetus salmincola schikhobalowi), paragonimiasis (paragonimus westermani ishunensis), and clonorchiasis (clonorchis sinensis). the tensest situation in the region has emerged with clonorchiasis, the pathogen of which is extending its area due to anthropogenic sources. in additi ... | 2015 | 26152030 |
| evaluation of modified formalin-ether concentration method using para tube in clinical settings. | conventional formalin-ether concentration method is a gold standard for the diagnosis of parasite infection. however, it may be time-consuming and laborious. we aimed to reveal the clinical usefulness of a modified formalin-ether concentration method using the para tube (ks corporation, korea) compared with the conventional method. a total of 117 fresh, unpreserved fecal samples composed to 90 negative controls and 27 positive controls with ova of diphyllobothrium latum/d. nihonkaiense, ova of c ... | 2015 | 26131417 |
| mapping of the putative epitope domain of clonorchis sinensis paramyosin (cspmy) recognized by cspmy-specific immunoglobulin g in sera of human clonorchiasis. | paramyosin of clonorchis sinensis (cspmy) is a myofibrillar protein localized in subtegumental muscle, tegument, and the muscle layer surrounding the intestine of the parasite. previously, we have identified that cspmy reacted with sera of human clonorchiasis and this protein had a potential as a candidate antigen for serodiagnosis of clonorchiasis. however, we also found that cspmy is able to bind to human immunoglobulin g (igg) in non-specific manners, which can affect the diagnostic value of ... | 2015 | 26099940 |
| [epidemiological characteristics and risk factors of hospital patients with clonorchiasis]. | to investigate the epidemiological characteristics and risk factors of clonorchiasis of hospital patients in heilongjiang province, so as to provide the evidence for formulating the control and prevention strategies. | 2015 | 26094415 |
| rare cause of jaundice in a post liver transplant patient. | a hepatitis b virus carrier suffering from acute flare of chronic hepatitis b infection underwent deceased-donor liver transplantation. he was put on the immunosuppressive agent tacrolimus. on routine follow-up, he was found to have abnormal liver function. computed tomography scan of the abdomen did not show any dilatation of the biliary system. liver biopsy showed scattered microabscesses, and a microgranuloma was detected. endoscopic retrograde cholangiography was performed and a biliary anas ... | 2015 | 26073470 |
| egg-specific expression of protein with dna methyltransferase activity in the biocarcinogenic liver fluke clonorchis sinensis. | despite recent reports regarding the biology of cytosine methylation in schistosoma mansoni, the impact of the regulatory machinery remains unclear in diverse platyhelminthes. this ambiguity is reinforced by discoveries of dna methyltransferase 2 (dnmt2)-only organisms and the substrate specificity of dnmt2 preferential to rna molecules. here, we characterized a novel dna methyltransferase, named csdnmt2, in a liver fluke clonorchis sinensis. the protein exhibited structural properties conserved ... | 2015 | 26036304 |