| changes in the fatty acid composition of phospholipids in tissues of farmed sea bass (dicentrarchus labrax) during an annual cycle. roles of environmental temperature and salinity. | we quantified seasonal effects on fatty acid composition of tissue phospholipids in farmed sea bass. major changes in percent phosphatidylethanolamine and phosphatidylcholine were observed in all tissues between february and march, and the phosphatidylcholine/phosphatidylethanolamine ratio was drastically reduced at this time. different changes in the fatty acid composition of total phospholipids were observed in all tissues examined. fish fed all year on the same commercial diet showed a signif ... | 2002 | 12431395 |
| determination of lead in biological samples by use of slurry sampling electrothermal atomic absorption spectrometry. | slurry-sampling electrothermal atomic absorption spectrometry has been applied to the determination of lead in several biological samples (fish and marine algae). the slurries were prepared both by magnetic shaking and microwave-heating and the effect of instrument operating conditions and slurry preparation conditions on the signal were examined. in addition, results from slurry sampling were compared with those obtained by microwave-assisted acid digestion of the same samples and no significan ... | 2002 | 12373397 |
| juvenile sea bass liver biotransformation induction and erythrocytic genotoxic responses to resin acids. | juvenile dicentrarchus labrax l. (sea bass) were exposed for 0, 2, 4, 6, and 8 h to abietic acid (aa) and dehydroabietic acid (dhaa) in concentration ranges 0, 0.0125, 0.025, 0.05, 0.1, 0.3, 0.9, and 2.7 microm. liver cytochrome p-450 content (p450) and liver ethoxyresorufin-o-deethylase activity (erod) were determined as phase i biotransformation biomarkers. genotoxicity was measured as erythrocytic micronuclei (emn) and nuclear abnormalities (ena). liver damage was assessed as liver alanine am ... | 2002 | 12297086 |
| effects of extender composition, cooling rate, and freezing on the motility of sea bass (dicentrarchus labrax, l.) spermatozoa after thawing. | a successful cryopreservation procedure for sperm must guarantee recovery of the morphological and functional characteristics of the cells following thawing so that preserved semen can to be used comparably with non-preserved semen. the aim of this work was to identify a species-specific freezing protocol for sea bass (dicentrarchus labrax) spermatozoa by optimising all the stages in the cryopreservation procedure. in the first stage of the experiments, the cryoprotectants and the relative conce ... | 2002 | 12237088 |
| post-prandial blood flow to the gastrointestinal tract is not compromised during hypoxia in the sea bass dicentrarchus labrax. | the hypothesis that the increase in post-prandial splanchnic blood flow will be reduced during hypoxia to prioritise blood flow to other organs was tested by measuring cardiac output and gut blood flow during a stepwise hypoxic challenge (five steps, from 20.6 to 3.9 kpa, 5 min of exposure to each level) before and after feeding (equivalent to 2.9% of body mass). splanchnic blood flow, both absolute and relative to cardiac output, increased after feeding. mean post-prandial gut blood flow increa ... | 2002 | 12177153 |
| s-100 protein is a selective marker for sensory hair cells of the lateral line system in teleosts. | the distribution of s100 protein in the neuromast of the lateral line system (lls) was investigated immunohistochemically in alevins of three species of teleosts (salmo trutta, salmo salar and dicentrarchus labrax), using a polyclonal antibody. in both the neuromasts of the canals, as well as in the pit organs, the hair cells, regarded as the specific sensory cells, displayed cytoplasmic immunoreactivity for s100 protein. conversely, the supporting cells, mantle cells and basal cells were devoid ... | 2002 | 12165395 |
| temperature sex determination in the european sea bass, dicentrarchus labrax (l., 1758) (teleostei, perciformes, moronidae): critical sensitive ontogenetic phase. | the temperature sex determination (tsd) mechanism in the european sea bass (dicentrarchus labrax l.) was studied in respect to: a) the tsd sensitivity during the different developmental stages; and b) the intrapopulation correlation of sex determination with the growth rate up to the end of the tsd-sensitive period. at the stage of half-epiboly, eggs from the same batch were divided into four groups and subjected to different thermal treatments: a) 15 degrees c (g15 group) and b) 20 degrees c (g ... | 2002 | 12115940 |
| branchial chloride cells in sea bass (dicentrarchus labrax) adapted to fresh water, seawater, and doubly concentrated seawater. | branchial chloride cells (cc) were studied in sea bass (dicentrarchus labrax) maintained in seawater (sw: 35 per thousand) or gradually adapted to and subsequently maintained in fresh water (0.2 per thousand) or doubly concentrated seawater (dsw: 70 per thousand). changes were observed in the location, number, and structure of ccs, that were discriminated by light, scanning, and transmission electron microscopy, as well as by immunofluorescence on the basis of their high na(+)/k(+)-atpase antige ... | 2002 | 12115915 |
| location and morphology of chloride cells during the post-embryonic development of the european sea bass, dicentrarchus labrax. | location and morphology of chloride cells were studied in the sea bass ( dicentrarchus labrax) from hatching to the juvenile stage to determine the development of the adult osmoregulatory function as seen in adult fish. during the studied developmental sequence changes were observed in the location, number, size and structure of these cells, that were studied by microscopy (light, scanning electron, transmission electron and confocal) and immunocytochemistry. chloride cells were found on the teg ... | 2002 | 12107490 |
| stress-induced changes of plasma antioxidants in aquacultured sea bass, dicentrarchus labrax. | antioxidant plasma activities of ascorbate, alpha-tocopherol and glutathione peroxidase were analysed in adult male sea bass, dicentrarchus labrax, in normal conditions and after hypoxia-recovery. in addition, tank measurements of temperature, ph, salinity and chlorine changes were carried out. ascorbate and alpha-tocopherol were measured using a high-pressure liquid chromatography method and glutathione peroxidase activity enzymatically. ascorbate and alpha-tocopherol showed a relationship with ... | 2002 | 12062211 |
| beta-naphthoflavone liver erod and erythrocytic nuclear abnormality induction in juvenile dicentrarchus labrax l. | juvenile dicentrarchus labrax l. (sea bass) was exposed to five different beta-naphthoflavone (bnf) concentrations-0, 0.1, 0.3, 0.9 and 2.7 microm-for 0, 2, 4, 6, 8, 16, 24, 48, 72, 144, and 216 h. a battery of biomarkers was investigated, such as liver ethoxyresorufin-o-deethylase (erod), liver cytochrome p450 (p450 content), liver aminotransferase (alt activity), liver somatic index (lsi), micronuclei (mn), and erythrocytic nuclear abnormality (ena) frequencies. juvenile d. labrax l. liver ero ... | 2002 | 12051810 |
| parasites of wild sea bass dicentrarchus labrax from norway. | thirteen wild sea bass from the oslo fjord in south-eastern norway were examined for parasites. nineteen species were found, comprising 5 protozoans, 1 monogenean, 8 digeneans, 1 cestode, 2 nematodes and 2 crustaceans. based on the similarity to the parasitic fauna of mediterranean sea bass, it is predicted that sea bass farmers in northern europe will experience the same parasite problems as sea bass farmers in warmer regions. | 2002 | 12033707 |
| [comparative analysis of genetic structure of 2 species of marine fish dicentrarchus labrax and dicentrarchus punctatus]. | we present here the genetic structure existing among five samples of the spotted sea bass dicentrarchus punctatus, and we compare it to what prevails in the common sea bass d. labrax, a congeneric species sampled on almost the same geographical range. a genetic distance tree inferred from the polymorphism at six microsatellite loci shows a distinct pattern for the two species. d. labrax samples appears to be genetically more homogeneous with a global fst of 3% as compared to the 10% observed at ... | 2002 | 12017769 |
| protein and arginine requirements for maintenance and nitrogen gain in four teleosts. | besides being an indispensable amino acid for protein synthesis, arginine (arg) is also involved in a number of other physiological functions. available data on the quantitative requirement for arg in different teleosts appear to show much variability. so far, there are very limited data on the maintenance requirements of indispensable amino acids (iaa) in fish. in the present study, we compared n and arg requirements for maintenance and growth of four finfish species: rainbow trout (oncorhynchu ... | 2002 | 12010584 |
| regulation of follicle-stimulating hormone (fsh) and luteinizing hormone (lh) gene expression by gonadotropin-releasing hormone (gnrh) and sexual steroids in the mediterranean sea bass. | the secretion of gonadotropins, the key reproductive hormones in vertebrates, is controlled from the brain by the gonadotropin-releasing hormone (gnrh), but also by complex steroid feedback mechanisms. in this study, after the recent cloning of the three gonadotropin subunits of sea bass (dicentrarchus labrax), we aimed at investigating the effects of gnrh and sexual steroids on pituitary gonadotropin mrna levels, in this valuable aquaculture fish species. implantation of sea bass, in the period ... | 2002 | 11997211 |
| residue depletion of thiamphenicol in the sea-bass. | the residue depletion of thiamphenicol (tap) was investigated in the sea-bass (dicentrarchus labrax) after 5 days' treatment with medicated food at a dose of 15 or 30 mg/kg bw/day. fish were sampled for blood and muscle + skin from 3 h until 14 days after treatment. thiamphenicol concentrations were assayed by high performance liquid chromatography. thiamphenicol concentrations measured 3 h after stopping treatment were 0.77 microg/ml and 0.91 (15 mg/kg dose) or 1.32 microg/ml and 1.47 microg/g ... | 2002 | 11874528 |
| temperature effects and genotype-temperature interactions on sex determination in the european sea bass (dicentrarchus labrax l.). | the effect of temperature on sex-ratios in 27 families of sea bass reared in the same tank from the fertilization stage onward was investigated. an excess of males (68%) was found in the groups that were reared at high temperature (mean +/- standard deviation: 20+/-1 degrees c) until they reached the mean size of 8.1 cm (standard length, 149 days post-fertilization [p.f.]). masculinization was higher (89% of males) in the groups maintained at low temperature (13 degrees c), from fertilization to ... | 2002 | 11857484 |
| induction of triploidy and gynogenesis in teleost fish with emphasis on marine species. | the induction of triploidy and gynogenesis by chromosome set manipulation has traditionally been studied more intensively in freshwater than in marine fish. in the last years, however, several studies have applied these manipulations in about a dozen marine species, including mainly sparids, moronids and flatfishes. this paper focuses on the methodologies used to induce, verify, and assess performance of both triploids and gynogenetics of these marine species. since many of them are batch spawne ... | 2001 | 11841165 |
| in vivo effects of phenolic compounds on blood parameters of a marine fish (dicentrarchus labrax). | sea bass (dicentrarchus labrax) were injected intraperitoneally once (single dose) or three times (fractionated dose) with phenol or oh-phenols (hydroquinone, resorcinol, and pyrocatechol). on the basis of the lethal doses, oh-phenols were more toxic than phenol, and pyrocatechol was the most powerful compound. hematological, metabolic and antioxidant blood parameters were measured 3 days after the end of the treatment. metabolic variations as specific effects on erythrocytes were revealed and d ... | 2000 | 11790355 |
| steroid metabolism by ovarian follicles of the sea bass dicentrarchus labrax. | ovarian samples from fear sea bass, dicentrarchus labrax, were collected for the in vitro incubations during the spawning period. follicles with fully developed vitellogenic oocytes showing central germinal vesicle (stage i follicles) and follicles with oocytes showing initial germinal vesicle migration (stage ii follicles) were treated with either (1) 20 microg sea bass hypophysis plus 50 ng 17-hydroxyprogesterone (17-p), (2) 20 microg hypophysis alone, (3) 50 ng 17-p alone and (4) media alone. ... | 2000 | 11790332 |
| environment and sex determination in farmed fish. | a plasticity of gonadal sex differentiation was reported in the 1930s following exogenous steroid treatments in fish, but demonstration that environmental factors (temperature, ph, density and social interactions) could influence the sex ratio in gonochoristic species has been relatively recent. in fish, as in reptiles and amphibians displaying environmental sex determination, the main environmental factor influencing sex seems to be temperature (tsd=temperature sex determination). in most therm ... | 2001 | 11738628 |
| temperature and myogenic factor transcript levels during early development determines muscle growth potential in rainbow trout (oncorhynchus mykiss) and sea bass (dicentrarchus labrax). | the influence of changes in environmental temperature on the mrna levels of myogenic regulatory factors (mrfs), i.e. myod and myogenin, as well as myosin heavy chain (myhc) were studied during early larval development in rainbow trout and sea bass. phosphoimager analysis of northern blots indicated that there is an optimum temperature for the rna transcript levels of mrf and myhc rna in trout and in sea bass larvae. in the trout strain studied, the highest concentration for mrf and myhc transcri ... | 2001 | 11683432 |
| comparison of the gonadal development and plasma levels of sex steroid hormones in diploid and triploid sea bass, dicentrarchus labrax l. | the goal of this study was to compare the reproductive physiology of triploid and diploid european sea bass (dicentrarchus labrax l.). gonads of diploid and triploid fish (males and females) were examined both microscopically and macroscopically, together with the plasma levels of the major sex steroids produced (testosterone and estradiol-17beta) when fish were adults. prior to sexual maturation, the gonadosomatic index (gsi) of triploid males was similar to that of diploids. however, the gsi i ... | 2001 | 11550186 |
| effects of beta-naphthoflavone on the cytochrome p450 system, and phase ii enzymes in gilthead seabream (sparus aurata). | the effect of beta-naphthoflavone (beta-nf) on several catalytic activities of cytochrome p450 (cyp) and phase ii enzymes putatively controlled by [ah]-receptor activation in the liver, heart and kidney of gilthead seabream, was investigated. in the liver, beta-nf treatment [intraperitoneal injection (i.p.) 50 mg/kg] resulted in an increase of cyp content, immunoreactive cyp 1a and methoxyresorufin-o-demethylase (merod), pentoxyresorufin o-depentylase (prod) and ethoxyresorufin-o-deethylase (ero ... | 2001 | 11544149 |
| development of the digestive tract of sea bass (dicentrarchus labrax l). light and electron microscopic studies. | the developing gut of sea bass was studied by light and electron microscopy, four phases being established. phase i, from hatching to the opening of the mouth, was a lecitotrophic period, in which the gut appeared as a straight undifferentiated tube lined by a simple epithelium that became stratified in the most caudal region. the epithelial cells increased in length towards the caudal zone, as did the number and height of the apical microvilli and the magnitude of the lamellar structures in the ... | 2001 | 11506432 |
| proteins from fish eggs that protect dna from acid precipitation and inhibit dna synthesis. | we partially characterized proteins that inhibit dna acid precipitation from various fish eggs (sparus aurata, dicentrarchus labrax, mugil cephalus and zeus faber). the active proteins were purified by acetone fractionation. the activity was found to be heat resistant. of bivalent cations tested only co(2+) and cu(2+) exerted a profound promoting effect in the activity from all fish. the protein fraction from sparus aurata inhibited dna synthesis in pcr performed by different dna polymerases. th ... | 2001 | 11489434 |
| critical evaluation of an intercalibration exercise undertaken in the framework of the med pol biomonitoring program. | the results of an intercalibration exercise among the laboratories participating in the med pol program for monitoring biological effects of pollutants along the mediterranean coasts are presented. three established biomarkers, i.e. lysosomal membrane stability, metallothionein concentration and ethoxyresorufin-o-deethylase (erod) activity, were intercalibrated. the stability of lysosomal membranes in mussels (mytilus galloprovincialis lam.) was assessed with a cytochemical method. the four part ... | 2000 | 11444011 |
| protein changes in post mortem sea bass (dicentrarchus labrax) muscle monitored by one- and two-dimensional gel electrophoresis. | this study was devoted to the identification of specific peptides and proteins which can be used as indicators of freshness in fish. the post mortem evolution of protein patterns in farmed sea bass muscle was monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and two-dimensional electrophoresis (2-de) after 0, 2, 4, and 6 days cold storage. sds-electrophoresis, of total proteins and proteins soluble in low-ionic-strength solutions, revealed the gradual disappearanc ... | 2001 | 11386667 |
| ig light chain variability in dnp(494)-klh immunised sea bass (dicentrarchus labrax l.): evidence for intra-molecular induced suppression. | the coding sequence of the sea bass light chain was obtained by sequential anchored pcr on a head kidney cdna library of a dnp(494)-klh immunised sea bass. the cdna sequence obtained codes for a leader peptide of 21aa and a mature igl chain of 223aa. both the amino acid sequence comparisons and neighbour-joining trees showed that the igl chain of sea bass obtained is of the l1/g type. to study the variability of the light chain, additional pcrs on the cdna library and cdna from pooled head kidne ... | 2008 | 11356219 |
| isolation and characterization of microsatellite loci for striped bass (morone saxatilis). | genetic variation has been difficult to detect in striped bass (morone saxatilis). therefore, we identified and characterized 13 microsatellite loci to provide additional genetic markers for striped bass. microsatellites were identified by screening a striped bass genomic library or by using primers developed for european sea bass (dicentrarchus labrax) microsatellite loci. we found that 6 of the 13 microsatellite loci were polymorphic in dna samples obtained from wild populations of striped bas ... | 2000 | 11246406 |
| cytoarchitectonic study of the brain of a perciform species, the sea bass (dicentrarchus labrax). ii. the diencephalon. | the cytoarchitecture of nuclei in the preoptic area, ventral thalamus, dorsal thalamus, epithalamus, hypothalamus, posterior tuberculum, synencephalon, and pretectum and the accessory optic nuclei was analyzed in a perciform teleost, the sea bass dicentrarchus labrax, by using serial sections stained with cresyl-violet. in general, the cytoarchitecture of the preoptic area, ventral and dorsal thalamus, epithalamus, and synencephalon resembles the histological pattern of other teleosts. however, ... | 2001 | 11223930 |
| cytoarchitectonic study of the brain of a perciform species, the sea bass (dicentrarchus labrax). i. the telencephalon. | a cytoarchitectonic analysis of the telencephalon of the sea bass dicentrarchus labrax, based on cresyl violet-stained serial transverse sections, is presented. rostrally, the brain of the sea bass is occupied by sessile olfactory bulbs coupled to telencephalic hemispheres. the olfactory bulbs comprise an olfactory nerve fiber layer, a glomerular layer, an external cellular layer, a secondary olfactory fiber layer, and an internal cellular layer. large terminal nerve ganglion cells are evident i ... | 2001 | 11223929 |
| neoparamoeba page, 1987: light and electron microscopic observations on six strains of different origin. | although amoebic gill disease (agd) has emerged as one of the most severe health problems in the fish industry, proof of the identity of agd agents from various localities is still missing. six strains of amoebae designated until recently as paramoeba species (the agents of agd) were studied in cultures by light and electron microscopy. although they were isolated from gills of different hosts (dicentrarchus labrax and scophthalmus maximus) and from distant localities, their morphology was ident ... | 2000 | 11206737 |
| effect of long-term oral administration of an immunostimulant diet on innate immunity in sea bass (dicentrarchus labrax). | immunostimulants represent a modern and promising tool in aquaculture, enhancing the resistance of cultured fish to disease and stress. this study investigated the effect of a combination of dietary glucans, alpha-tocopherol and ascorbic acid on the innate immune response of cultured sea bass (dicentrarchus labrax). after 5 weeks of adaptation on a commercial diet containing 100 p.p.m. ascorbic acid and 200 p.p.m. alpha-tocopherol, sea bass were switched to a diet supplemented with 2% beta-1.3/b ... | 2000 | 11204129 |
| cadmium and copper display different responses towards oxidative stress in the kidney of the sea bass dicentrarchus labrax. | copper and cadmium were i.p. injected into the fish dicentrarchus labrax. cu as cd-treated fish showed an enlargement of the lysosomal membrane of the kidney (pronephros), cu being more toxic than cd. following injection, metal uptake, measured in muscle, liver and kidney, was much higher with cadmium treatment than with copper, the kidney being the main accumulating organ of cadmium in which metal accumulation is correlated with increased zinc level, suggesting metallothionein induction. in viv ... | 2000 | 10686325 |
| ontogeny of b and t cells in sea bass (dicentrarchus labrax, l.). | monoclonal antibodies specific to sea bass ig heavy (wdi 1) and light (wdi 3) chains and t cells (dlt15) were used in an ontogenetic study of sea bass by flow cytometry and immunocytochemistry. the influence of weight and age, as well as season, on b cell development was studied in the fastest and slowest growing offspring from the same spawn (5-305 days post hatch: dph). additionally, b and t cell development was followed in samples of different offspring (5-137 dph). the results suggest that d ... | 2000 | 11081436 |
| cdna cloning and sequence of european sea bass (dicentrarchus labrax) somatolactin. | three cdna clones encoding for european sea bass somatolactin (sl) were obtained by rt-pcr and 3' race of rna of pituitary origin. clone 1 was 582 bp in length, and included a part of the signal peptide and the 5' end of the mature protein. clone 2 (1075 bp) included a fragment of the coding sequence and the 3' untranslated region, which was 888 bp in length and contained two putative polyadenylation signals (aataaa) at 12-17, and 202-207 nucleotides upstream of the poly (a) tail. clone 3 was 62 ... | 2000 | 11079372 |
| molecular evolution of the neuropeptide y (npy) family of peptides: cloning of three npy-related peptides from the sea bass (dicentrarchus labrax). | neuropeptide y (npy) is a 36-amino-acid peptide that is widely and abundantly expressed in the central nervous system of all vertebrates investigated. related peptides have been found in various vertebrate groups: peptide yy (pyy) is present in gut endocrine cells of many species and pancreatic polypeptide (pp) is made in the pancreas of all tetrapods. in addition, a fish pancreatic peptide called py has been reported in three species of fishes. the evolutionary relationships of fish py have bee ... | 2000 | 11062329 |
| potassium channels in primary cultures of seawater fish gill cells. i. stretch-activated k(+) channels. | previous studies using the patch-clamp technique demonstrated the presence of a small conductance cl(-) channel in the apical membrane of respiratory gill cells in primary culture originating from sea bass dicentrarchus labrax. we used the same technique here to characterize potassium channels in this model. a k(+) channel of 123 +/- 3 ps was identified in the cell-attached configuration with 140 mm kcl in the bath and in the pipette. the activity of the channel declined rapidly with time and co ... | 2000 | 11049847 |
| characterization of neuropeptide y expression in the brain of a perciform fish, the sea bass (dicentrarchus labrax). | the distribution of neuropeptide y (npy) gene expression was mapped in the brain of the sea bass (dicentrarchus labrax) by in situ hybridization with 35s-utp labeled crna probes. gene expression was mainly detected within the forebrain, although npy mrna transcripts were also localized in the tectum and tegmentum mesencephali and posterior brain. new npy-expressing nuclei were found in the dorsal and ventral telencephalon, preoptic area, tuberal hypothalamus, synencephalon, tegmentum mesencephal ... | 2000 | 11036237 |
| temperature effect on muscle growth of the axial musculature of the sea bass (dicentrarchus labrax l.). | in order to determine the temperature effect on the axial muscle growth of sea bass, a stock of larvae was subjected to the following incubation and cultivation temperatures, respectively: 15 degrees c/ambient, 15/17 degrees c, 17 degrees c/ambient and 17/17 degrees c. in all groups the cross-sectional area of white and red muscles and the number and average area of the white and red muscle fibres were quantified. results showed that the embryonic period, pre-larval phase and the end of metamorp ... | 2000 | 11008371 |
| peptide yy (pyy) and fish pancreatic peptide y (py) expression in the brain of the sea bass (dicentrarchus labrax) as revealed by in situ hybridization. | tetrapod vertebrates express three neuropeptide y (npy)-related peptides: npy, peptide yy (pyy), and pancreatic polypeptide (pp). both npy and pyy mrna have been localized in the brain of tetrapods whereas pp expression is restricted to the pancreas. some teleost fish commonly produce npy and pyy but pancreatic peptide y (py) instead of pp. both npy and pyy mrnas are widely distributed in the brain of non-tetrapod species, but no information about py central expression is available. in the prese ... | 2000 | 10982463 |
| immunopurification of t-cells from sea bass dicentrarchus labrax (l.). | the monoclonal antibody dlt15, specific for thymocytes and peripheral t-cells of the teleost fish dicentrarchus labrax (sea bass), was used to purify immunoreactive cells from blood and gut-associated lymphoid tissue. the purification was performed by immuno-magnetic sorting of leucocyte fractions enriched by percoll density gradient centrifugation, and the purity of the isolated cells was estimated by cytofluorimetric analysis. following a single step, the percentage of dlt15-purified cells was ... | 2000 | 10938743 |
| evaluation of immunoglobulins produced in vitro by head-kidney leucocytes of sea bass dicentrarchus labrax by immunoenzymatic assay. | | 2000 | 10938726 |
| evidence of temperature-dependent sex determination in the european sea bass (dicentrarchus labrax l.). | to test the hypothesis that sex determination in the european sea bass (dicentrarchus labrax l.) can be affected by the incubating temperature during the very early developmental stages, eggs from the same batch of spontaneously spawned broodstock were divided at the stage of half-epiboly into three groups according to rearing temperature: g13 = 13 degrees c, g15 = 15 degrees c, and g20 = 20 degrees c. temperature treatment lasted until the middle of metamorphosis (17-18 mm total length, [tl]), ... | 2000 | 10900442 |
| fragmentation of sea bass populations in the western and eastern mediterranean as revealed by microsatellite polymorphism. | we studied the genetic structure at six microsatellite loci of the mediterranean sea bass (dicentrarchus labrax) on 19 samples collected from different localities in the western and eastern mediterranean basins. significant divergence was found between the two basins. the distance tree showed two separate clusters of populations which matched well with geography, with the noticeable exception of one egyptian sample which grouped within the western clade, a fact attributable to the introduction o ... | 2000 | 10853737 |
| aflp analysis confirms exclusive maternal genomic contribution of meiogynogenetic sea bass (dicentrarchus labrax l.). | meiogynogenesis was induced in the european sea bass dicentrarchus labrax l. by fertilizing eggs with uv-irradiated sperm followed by inhibition of the second meiotic division by a cold shock. putative gynogenetic progeny derived from three groups of breeders were analyzed for maternal inheritance using amplified fragment length polymorphism (aflp) markers. discrimination of fingerprints was based on male-specific bands, which were absent in females. four of 64 msei/ecori primer pairs used to an ... | 2000 | 10852810 |
| cellular and humoral immune response of european sea bass (dicentrarchus labrax l.) (teleostei: serranidae) immunized with sphaerospora dicentrarchi (myxosporea: bivalvulida). | the immune response of european sea bass after intracaelomic immunization with sphaerospora dicentrarchi was studied. fish were injected with s. dicentrarchi spores (dic), with spores plus adjuvant (dic + fca), with adjuvant alone (fca) or with pbs. several parameters of the immune response were measured. serum lysozyme increased significantly in dic fish 1 week after immunization (p.i.) and it remained significantly higher in dic + fca fish 4 weeks p.i., and in dic fish 8 weeks p.i. than in pbs ... | 2000 | 10840976 |
| neutral calcium-activated proteases from european sea bass (dicentrarchus labrax l.) muscle: polymorphism and biochemical studies. | calcium-dependent proteinases or calpains were studied in fish muscle. hydrophobic chromatography, followed by anion-exchange chromatography of the soluble fraction of sea bass white muscle proteins, resulted in three peaks of calcium-dependent protease activity at neutral ph (a, b and c). they are all neutral cysteine calcium-activated proteinases and can, therefore, be classified as calpain-like enzymes. from the ca2+ concentration required for activity, a is a mu-calpain, and b and c are m-ca ... | 2000 | 10840644 |
| trk neurotrophin receptor-like proteins in the teleost dicentrarchus labrax. | in recent years, data have accumulated suggesting that the role of neurotrophins and trk receptors may not be limited to the nervous system, and the presence of these substances has been detected in a variety of vertebrate and invertebrate non-nervous tissues. this study was designed to map the expression of immunoreactivity (ir) for trk-like proteins in alevins of the teleost dicentrarchus labrax, with particular emphasis on non-nervous structures. we used antibodies against specific epitopes o ... | 2000 | 10805070 |
| oral single-dose pharmacokinetics of thiamphenicol in the sea-bass (dicentrarchus labrax). | | 2000 | 10747244 |
| do discrepancies between microsatellite and allozyme variation reveal differential selection between sea and lagoon in the sea bass (dicentrarchus labrax)? | in the present study the genetic structure of dicentrarchus labrax (14 samples from the mediterranean) was analysed at six microsatellite loci, in order to test the hypothesis that some enzymatic loci undergo selection between marine and lagoon habitat. eight of the 14 samples were analysed at both microsatellite and allozyme markers. the analysis of the genetic variation among the mediterranean samples showed that (i) &fcirc;st values obtained with the six microsatellite loci were much smaller ... | 2000 | 10736048 |
| determination of vitamin e in eggs and during the larval development of the sea bass, dicentrarchus labrax by high performance liquid chromatography. | | 2000 | 10705951 |
| hepatic ascorbic acid saturation is the most stringent response criterion for determining the vitamin c requirement of juvenile european sea bass (dicentrarchus labrax). | our main objective was to verify whether the dietary ascorbic acid (aa) requirement of juvenile european sea bass (dicentrarchus labrax) varies as a function of different physiological needs. practical diets with eight (0, 5, 10, 20, 40, 80, 160, 320 mg aa/kg diet) levels of ascorbic acid polyphosphate were fed to sea bass (mean weight: 0.7 g) for 15 wk. at the beginning and at the end of the feeding trial, tissues were sampled for vitamin c and hydroxyproline (hypro) analysis. dose-dependent re ... | 2000 | 10702594 |
| the partial substitution of digestible protein with gelatinized starch as an energy source reduces susceptibility to lipid oxidation in rainbow trout (oncorhynchus mykiss) and sea bass (dicentrarchus labrax) muscle. | we evaluated the influence of dietary gelatinized starch and protein on the fatty acid composition of muscle in rainbow trout and european sea bass and on the susceptibility of flesh to lipid peroxidation. the possibility that flesh peroxidation could be accounted for by lipogenesis and the deposition of fat was also explored. the inclusion of gelatinized starch in the diet of rainbow trout improved growth with respect to that observed in fish fed crude starch (p<.001). this was especially notic ... | 1999 | 10641880 |
| purification and characterization of glutathione transferases from the sea bass (dicentrarchus labrax) liver. | two forms of glutathione transferase were purified from liver cytosol of the sea bass (dicentrarchus labrax) by gsh-sepharose affinity chromatography followed by chromatofocusing. the major enzyme (dl-gst-6.7; 75% of total activity bound to the column) has a pi value of 6.7 and is composed of two subunits of apparent molecular mass 26.5 kda. the minor enzyme (dl-gst-8.2; 25% of total activity bound to the column) has a pi value of 8.2 and is composed of two subunits of molecular mass 23.5 kda. b ... | 2000 | 10620369 |
| blood cells of sea bass (dicentrarchus labrax l.). flow cytometric and microscopic studies. | studies of fish blood cells made to date presented numerous problems derived from both the nomenclature and the techniques used. a combination of quantitative and morphological methods is needed if the classification of fish blood cells is to advance from it present provisional state. the aim of the present paper was first to isolate sea bass blood cell populations by flow cytometry and second to characterize then microscopically. blood cell populations from sea bass (dicentrarchus labrax l.) we ... | 2000 | 10603451 |
| the relationship between the effects of uv light and thermal shock on gametes and the viability of early developmental stages in a marine teleost fish, the sea bass (dicentrarchus labrax l.). | to improve the efficiency of gynogenetic induction, the effects of uv light and thermal shock on gametes were investigated in the sea bass. exposure of sperm to uv light (>/=15 000 erg mm-2) reduced the amount of motile spermatozoa, without affecting the duration of motility in the spermatozoa that remained motile. the hertwig effect was elicited in eggs fertilized with sperm exposed to >/=35 000 erg mm-2 of uv light, indicating the inactivation of the dna of the spermatozoa while retaining thei ... | 1999 | 10583540 |
| induction of nodavirus disease in seabass, dicentrarchus labrax, using different infection models. | the aim of this study was to investigate the susceptibility of juvenile and adult seabass, which are generally thought to be refractory to nodavirus. moreover, preliminary immunological studies were performed to examine the immune response of adult seabass. successful transmission of the disease was experimentally demonstrated in juvenile and adult seabass as ascertained by the presence of the clinical signs of the disease, re-isolation of the virus in the ssn-1 cell line and subsequent confirma ... | 1999 | 10509719 |
| natural outbreak of viral encephalopathy and retinopathy in juvenile sea bass, dicentrarchus labrax: study by nested reverse transcriptase-polymerase chain reaction. | in order to improve the sensitivity of the diagnosis of viral encephalopathy and retinopathy (ver) in sea bass, a nested reverse transcriptase-polymerase chain reaction (rt-pcr) detection method was developed. the reverse transcription step and the first stage pcr were performed using outer primers specific for the coat protein gene, whereas a new primer set was used as inner primers for the second stage pcr. fish were collected just before, during and after a ver outbreak occurring in a mediter ... | 1999 | 10509711 |
| effects of osmolality, morphology perturbations and intracellular nucleotide content during the movement of sea bass (dicentrarchus labrax) spermatozoa. | sea bass spermatozoa are maintained immotile in the seminal fluid, but initiate swimming for 45 s at 20 degrees c, immediately after dispersion in a hyperosmotic medium (1100 mosm kg-1). the duration of this motile period could be extended by a reduction of the amplitude of the hyperosmotic shock. five seconds after the initiation of motility, 94.4 +/- 1.8% of spermatozoa were motile with a swimming velocity of 141.8 +/- 1.2 microns s-1, a flagellar beat frequency of 60 hz and a symmetric type o ... | 1999 | 10505062 |
| toxicity of fatty acid 18:5n3 from gymnodinium cf. mikimotoi: i. morphological and biochemical aspects on dicentrarchus labrax gills and intestine. | we present evidence for the toxic effects of fatty acid 18:5n3 (octadecapentaenoic acid) in the gills and intestine of the sea bass dicentrarchus labrax. light microscopic observation of gills showed strong mucus production and alteration of ionocytes. the mg- and na,k-atpase activities were inhibited, with ic50 values of 10(-3) and 1.6 x 10(-4) m, respectively. results are discussed in relation to osmoregulation. | 2007 | 10439343 |
| high dietary lipid levels enhance digestive tract maturation and improve dicentrarchus labrax larval development. | this study was designed to determine the nutritional lipid requirement of seabass larvae and to understand the effects of dietary fat concentration on their digestive tract maturation. seabass (dicentrarchus labrax) larvae were fed, from d 15 to 38 of life, one of five isonitrogenous compound diets with different lipid levels, ranging from 10 to 30 g/100 g. the higher the lipid level, the greater the growth and survival of the larvae (p < 0.05). the lipolytic enzymes assayed, lipase and phosphol ... | 1999 | 10356086 |
| comparative study of viral encephalopathy and retinopathy in juvenile sea bass dicentrarchus labrax infected in different ways. | the transmission of viral encephalopathy and retinopathy (ver) was investigated in juvenile sea bass (3 g) dicentrarchus labrax by using cell culture supernatant (ssn-1 cell line) containing nodavirus. five methods of infection were tested: intramuscular injection (im), intraperitoneal injection (ip), oral infection, bath exposure and cohabitation of healthy fish with infected fish. some differences were observed in time of disease onset and severity of symptoms depending on the mode of infectio ... | 1999 | 10349548 |
| tissue-specific induction and inactivation of cytochrome p450 catalysing lauric acid hydroxylation in the sea bass, dicentrarchus labrax. | microsomal cytochrome p450-dependent lauric acid hydroxylase activities were characterized in liver, kidney, and intestinal mucosa of the sea bass (dicentrarchus labrax). microsomes from these organs generated (omega-1)-hydroxylauric acid and a mixture of positional isomers including (omega)-, (omega-2)-, (omega-3)- and (omega-4)-hydroxylauric acids, which were identified by rp-hplc and gc-ms analysis. peroxisome proliferators, such as clofibrate and especially di(2-ethylhexyl) phthalate, increa ... | 1999 | 10327614 |
| cloning of cyp4f7, a kidney-specific p450 in the sea bass dicentrarchus labrax. | a cdna sequence coding for a cytochrome p450 of the cyp4f subfamily was isolated from total rna of sea bass kidney by rapid amplification of cdna ends. the full length sequence coded for a protein of 526 amino acids. the amino acid sequence shared 39% to 56% residue identities with the mammalian cyp4f sequences, and thus was named cyp4f7 (accession number af123541). rna blot analysis using cyp4f7 cdna as a probe indicated that the corresponding mrna was only detected in kidney. expression in the ... | 1999 | 10222252 |
| effect of clofibrate, a peroxisome proliferator, in sea bass (dicentrarchus labrax), a marine fish. | the effects of 35 or 70 mg/kg clofibrate on some peroxisomal, mitochondrial, and microsomal enzymes (markers of peroxisome proliferation) in liver, gill, and kidney of sea bass following 2 weeks i.p. treatment have been studied. induction of lauric acid hydroxylase, udp-glucuronyl transferase, palmitoyl-coa oxidase, carnitine-palmitoyl-transferase, p-nitrophenylacetate hydrolase, and benzaldehyde and propionaldehyde dehydrogenase activities was not observed in any case. the clofibrate administra ... | 1999 | 10092449 |
| regulation of cl- secretion in seawater fish (dicentrarchus labrax) gill respiratory cells in primary culture. | 1. primary cultures of sea bass (dicentrarchus labrax) gill cells grown on permeable membranes form a highly differentiated tight epithelium composed of respiratory-like cells. this preparation was also found to provide a functional model for investigating the hormonal regulation of cl- secretion. 2. in control conditions, i.e. in the absence of hormones or other stimuli, the cultured epithelium showed a short-circuit current (isc) of 8.8 +/- 0.4 microa cm-2, a transepithelial potential (vt) of ... | 1999 | 10087336 |
| a monoclonal antibody against chorion proteins of the sea bass dicentrarchus labrax (linnaeus, 1758): studies of chorion precursors and applicability in immunoassays. | the monoclonal antibody dle7 was obtained against 44- to 50-kda polypeptides solubilized from the vitelline envelope of the mediterranean sea bass dicentrarchus labrax. in western blot analysis of chorion lysates it recognized cross-reactive bands at 44 kda, 48 kda, and 110 kda. previous affinity blotting with concanavalin-a showed that most of solubilized bands were glycosylated. enzymatic deglycosylation of chorion proteins followed by western blot analysis with dle7 showed that the 48-kda and ... | 1999 | 10084949 |
| immunodetection of lymphocyte subpopulations involved in allograft rejection in a teleost, dicentrarchus labrax (l.). | monoclonal antibodies which recognize antigenic determinants expressed by t-cells and ig-bearing cells, respectively, allowed lymphocyte subpopulations involved in allograft rejection of muscle transplants to be identified in the teleost fish dicentrarchus labrax (l.). the monoclonal antibody dlt15 first allowed recognizing t-cells involved in an in vivo antigen-driven cellular response in teleosts. immunohistochemical studies showed a high density of lymphocytes in allografts and provided evide ... | 1999 | 9973538 |
| molecular cloning of a cyp1a cdna from the teleost fish dicentrarchus labrax. | a cdna encoding for cytochrome p450 1a has been cloned in the marine teleost fish dicentrarchus labrax. this fish, common in the mediterranean, was chosen since it is considered a good sentinel species. moreover, biomarkers of exposure to organic contaminants (such as erod) are often measured in this species and make it possible to evaluate the quality of waters. for cloning purposes, rnas were extracted from the liver of benzo[a]pyrene (bap)-treated animals and used as template in degenerate rt ... | 1998 | 9972466 |
| osmoregulation and salinity effects on the expression and activity of na+,k(+)-atpase in the gills of european sea bass, dicentrarchus labrax (l.). | the european sea bass, dicentrarchus labrax, tolerates salinities ranging from freshwater (fw) to hypersaline conditions. in two experiments, we analysed changes in plasma ions, muscle water content (mwc), gill na+,k(+)-atpase activity, and alpha-subunit mrna expression during the course of acclimation from 15 ppt salt water to fw or high salinity seawater (hssw). in experiment 1, fish (6.2 +/- 1.1 g) were acclimated from 15 ppt to either fw, 5, 15, 25, 50, or 60 ppt sw and sampled after 10 days ... | 1998 | 9755480 |
| time-course studies on plasma glucose, insulin, and cortisol in sea bass (dicentrarchus labrax) held under different photoperiodic regimes. | daily variations of insulin, cortisol, and glucose are studied in animals adapted to two different photoperiodic regimes, with the intervals between feeding times and photoperiodic events kept constant. data support the existence of a daily rhythm of plasma glucose which seems to be photoperiodic. in contrast, the daily patterns of insulin in sea bass seem to be mainly influenced by feeding time; however, an effect of photoperiod can not be excluded. when the digestive tract is absent of food, i ... | 1998 | 9748089 |
| two polymorphic microsatellite markers in the european seabass, dicentrarchus labrax (l.). | | 1998 | 9699281 |
| cloning of neuropeptide y, peptide yy, and peptide y from sea bass (dicentrarchus labrax), a marine teleost. | | 1998 | 9629200 |
| hepatic steatosis in artificially fed marine teleosts. | in a comparison of livers in fish (sparus auratus and dicentrarchus labrax) feeding on natural sources of food with livers of artificially fed animals, a much higher c18:1/c22:6 ratio was observed in the latter. staining livers with oil red o showed extensive steatosis in artificially fed fish, but not in those naturally fed. juvenile artificially fed fish showed a more extensive steatosis and a higher mortality rate. in steatotic fish fed a natural diet for 2 months, the liver exhibited extensi ... | 1998 | 21361972 |
| expression of lymphocyte antigenic determinants in developing gut-associated lymphoid tissue of the sea bass dicentrarchus labrax (l.). | the monoclonal antibodies dlt15 and dlig3, which recognize antigenic determinants expressed by t cells and ig-bearing cells, respectively, allowed the development of gut-associated lymphoid tissue of the teleost fish dicentrarchus labrax (l.) to be studied. dlt15-immunoreactive cells were first detected in the epithelium of the stomach and intestine at day 30 post-hatching of fish maintained at 16 degrees c. at that age, positive cells were found only in the thymus. between day 44 and day 81 pos ... | 1997 | 9453366 |
| high-performance liquid chromatographic determination of oxolinic acid in the plasma of seabass (dicentrarchus labrax) anaesthetized with 2-phenoxyethanol. | a high-performance liquid chromatographic (hplc) method for the determination of oxolinic acid (oa) in the plasma of seabass (dicentrarchus labrax) anaesthetized with 2-phenoxyethanol is described. the samples were extracted and cleaned up by a solid-phase extraction procedure using c18 extraction cartridges. after the eluent was evaporated, the dry residue was dissolved in 1/15 m phosphate buffer. oa was determined by using an isocratic hplc method with uv detection at 340 nm. seabass drug-free ... | 1997 | 9367215 |
| molecular speciation of fish sperm phospholipids: large amounts of dipolyunsaturated phosphatidylserine. | the molecular species compositions of the main diacyl phosphoglyceride classes and ether-linked subclasses from sperm of three species of fish, sea bass dicentrarchus labrax, atlantic salmon salmo salar and chinook salmon onchorhynchus tsawytscha, were determined. the phospholipids from sperm were highly unsaturated, dipolyunsaturated fatty acid (dipufa) molecular species comprised 64.6 to 71.8% of phosphatidylserine (ps), 10.1 to 17.4% of phosphatidylethanolamine (pe), and 3.3 to 10.1% of phosp ... | 1997 | 9358435 |
| chloride channels in primary cultures of seawater fish (dicentrarchus labrax) gill. | patch-clamp experiments were undertaken on primary cultures of respiratory cells originating from sea bass (dicentrarchus labrax) gills. a small-conductance cl- channel of 8 ps was characterized in cell-attached configuration with 140 mm n-methyl-d-glucamine-cl in the pipette and bath solutions. no activity was recorded below a membrane holding potential of +20 mv (-vp, referenced to the pipette solution), and the channel showed an inward rectification. in the inside-out configuration the cl- ch ... | 1997 | 9316408 |
| fish nodavirus lytic cycle and semipermissive expression in mammalian and fish cell cultures. | in this study, dicentrarchus labrax encephalitis virus (dlev), which causes sea bass encephalitis, was propagated in cell culture, thus allowing study of its lytic cycle. dlev infection of mammalian and fish cells induced different patterns of expression of capsid proteins, which were assembled as virus-like particles, accumulating in the cytoplasm either as diffuse masses or in vesicles, as shown by electron microscopy. these particles correspond to virions, as shown by their ability to induce ... | 1997 | 9188647 |
| immunocytochemical detection and cytomorphology of lymphocyte subpopulations in a teleost fish dicentrarchus labrax. | the monoclonal antibodies dlt15 and dlig3 directed against thymocytes and serum immunoglobulins of the sea bass (dicentrarchus labrax, l.) were used to study cells from the thymus, head kidney, spleen, gut-associated lymphoid tissue and peripheral blood leukocytes of this fish by immunofluorescence and pre-embedding immunoelectron microscopy. immunofluorescence and flow cytometry of leukocyte fractions revealed a large number of dlt15-positive cells in the thymus (approximately 80%) and intestin ... | 1997 | 9182611 |
| unusual responses to light and darkness of ocular melatonin in european sea bass. | regulation by light and darkness of melatonin rhythms in the plasma and eye of the european sea bass (dicentrarchus labrax) was studied. during light-dark cycles, plasma and ocular melatonin exhibited day-night changes with higher levels at mid-dark and at mid-light, respectively. circulating melatonin levels were low in constant light but high in constant darkness (dd); ocular melatonin levels showed the reverse pattern. plasma melatonin exhibited circadian rhythm for 1 cycle but the rhythm was ... | 1997 | 9189904 |
| partial substitution of di- and tripeptides for native proteins in sea bass diet improves dicentrarchus labrax larval development. | to determine whether incorporation of peptides into diets can improve larval development, sea bass (dicentrarchus labrax) larvae were fed for 21 d one of three isonitrogenous, isoenergetic semipurified diets in which enzymatic hydrolysate (75% di- and tripeptides) of fish meal proteins was substituted for 0, 20 or 40% of native fish meal proteins. growth and survival were significantly greater (p < 0.05) in larvae fed peptide diets compared to those fed only native protein, with the best perform ... | 1997 | 9109612 |
| histochemistry of carbohydrates, proteins and lipids during swimbladder development in seabream, sparus aurata and seabass, dicentrarchus labrax. | some histochemical aspects of carbohydrates, proteins and lipids were studied in the swimbladder of seabream, sparus aurata and seabass, dicentrarchus labrax. in both species, swimbladder development occurs in two stages: (1) inflation and (2) expansion, respectively. no important differences in histochemical distribution of glucidic, proteic and lipidic macromolecules were observed during the swimbladder development. glycoproteins containing mannose and/or glucose sugar are present in the swimb ... | 1997 | 9491314 |
| [modulation of respiratory activity of renal macrophages in sea bass (dicentrarchus labrax) by chronic exposure to sublethal concentration of ammonia]. | sea bass (dicentrarchus labrax) were exposed for 71 days to three different ammonia concentrations corresponding to 0, 15 and 25% of the lethal threshold concentration causing the death of half a fish population in 96 hours. the study of the respiratory burst from sea bass renal macrophages showed that the luminol dependent chemiluminescence (cl) emitted following stimulation with mezerein is higher when fishes were previously exposed to ammonia. therefore, it seemed that chronically exposure of ... | 1997 | 9404463 |
| identification of vitellogenin receptors in the ovary of a teleost fish, the mediterranean sea bass (dicentrarchus labrax). | the characterization of vitellogenin (vtg) receptors in ovarian membranes from vitellogenic female sea bass (dicentrarchus labrax) is described. incubation of membrane proteins with radiolabeled vtg (125i-vtg) after sds-electrophoresis showed specific binding of 125i-vtg to a protein band of 100 kda. filter binding assays showed that binding of 125i-vtg to membrane receptors was saturable with increasing amounts of 125i-vtg. scatchard analysis of the saturation data revealed a single class of bi ... | 1997 | 9115596 |
| cell culture isolation of piscine neuropathy nodavirus from juvenile sea bass, dicentrarchus labrax. | a virus causing a vacuolating encephalopathy and retinopathy in juvenile sea bass, dicentrarchus labrax, was isolated from brain tissue in a fish cell line (ssn-1) derived from striped snakehead, channa striatus. the isometric, non-enveloped, 30 nm diameter virus particles were resistant to ph 2-9 and heating at 56 degrees c for 30 min. infectious particles had a buoyant density of approximately 1.31 g/cm3 in cscl. two structural polypeptides of molecular mass 40 and 42 kda were identified and t ... | 1996 | 8811004 |
| cytotoxicity of phenolic compounds on dicentrarchus labrax erythrocytes. | | 1996 | 8661899 |
| amylase and trypsin responses to intake of dietary carbohydrate and protein depend on the developmental stage in sea bass (dicentrarchus labrax) larvae. | sea bass (dicentrarchus labrax) larvae were fed from day 15 to day 35 with 4 isoenergetic formulated diets that varied in protein (30, 40, 50, 60%) and carbohydrate (37, 27, 17, 7%) content. the diets were designated as p30, p40, p50 and p60, respectively. best growth and survival were noted for p50 larvae. poor growth and survival were observed for p30 larvae. the specific activity of amylase increased in direct relation to the dietary carbohydrate level from day 18 onwards. this increase was t ... | 1996 | 24194142 |
| characterization of mevalonate metabolism in the sea bass (dicentrarchus labrax l) liver. | the activities of mevalonate kinase, mevalonate 5-phosphate kinase and mevalonate 5-pyrophosphate decarboxylase, were examined in sea bass (dicentrarchus labrax l) liver. the activities of the three enzymes were studiedin vitro in relation to the influence of protein content, time of incubation, ph, temperature, mevalonate, atp and mg(++) concentration. protein content in the assay medium affected the three enzymes differently. mevalonate kinase, mevalonate 5-phosphate kinase, and mevalonate 5-p ... | 1996 | 24194139 |
| immunohistochemical and ultrastructural evidence of adrenal chromaffin cell subtypes in sea bass dicentrarchus labrax (l.). | histology demonstrated chromaffin cells in the head kidney and opisthonephros of the sea bass. immunohistochemistry showed the catecholamine-synthesizing enzymes tyrosine hydroxylase and dopamine beta-hydroxylase in these cells. phenlethanolamine n-methyltransferase was detected in a fraction of the chromaffin cells in the head kidney (59%) and in the opisthonephros (54%). distinct noradrenaline- and adrenaline-synthesizing cells are therefore suggested. ultrastructural analysis confirmed the ex ... | 1996 | 8860315 |
| occurrence of somatostatin and insulin immunoreactivities in the stomach of sea bass (dicentrarchus labrax l.): light and electron microscopic studies. | somatostatin (sst)- and insulin (ins)-immunoreactive (ir) cells were identified in the gut of sea bass (dicentrarchus labrax) by immunofluorescence double staining and peroxidase-antiperoxidase (pap) techniques for light microscopy and by immunogold method for electron microscopy using antisera to mammalian and fish peptides. sst-14 and sst-25 immunoreactivities coexisted in cells mainly located among the mucous neck cells of the gastric glands. preabsorption controls showed that some sst-25- an ... | 1996 | 8860304 |
| nonspecific cell-mediated cytotoxicity in the seawater teleosts (sparus aurata and dicentrarchus labrax): ultrastructural study of target cell death mechanisms. | in higher vertebrates, cytolytic lymphocytes lyse their targets by the mechanisms of cell death called necrosis and apoptosis. however, the mechanisms of target cell death mediated by fish cytotoxic cells have not been established. we report the ultrastructure of target cell death mediated by the seawater teleosts gilthead seabream (sparus aurata) and sea bass (dicentrarchus labrax). | 1996 | 8694285 |
| a standard experimental diet for the study of fatty acid requirements of weaning and first ongrowing stages of the european sea bass dicentrarchus labrax l.: comparison of extruded and extruded/coated diets. | the stability of the coated lipid fraction of a standard weaning diet was verified by comparing growth and fatty acid incorporation for european sea bass fed diets with an identical formulation but prepared either by extrusion/coating or extrusion only. for each type of experimental diet, a (n-3) hufa level of 1 and 2.5% of the dry diet was evaluated. european sea bass dicentrarchus labrax l. were weaned and consequently reared for 23 days on the experimental diets in a recirculation system. a c ... | 1996 | 8766973 |
| maturation of the pancreatic and intestinal digestive functions in sea bass (dicentrarchus labrax): effect of weaning with different protein sources. | the maturation of the digestive functions in sea bass (dicentrarchus labrax) larvae was evaluated by the enzymatic profile of pancreas and intestine brush border membranes. sea bass larvae were weaned at day 25 with three simplified diets different by their protein nature: 100% fish meal (fp), 100% casein mixture (cp) and 50% fish meal-50% casein mixture (cfp). the casein mixture contained 35% of hydrolysate. the control group was fed live preys. the specific activity of amylase decreased with a ... | 1995 | 24197639 |
| proteolysis by juvenile sea bass (dicentrarchus labrax) gastrointestinal enzymes as a method for the evaluation of feed proteins. | protein digestibility by gastrointestinal homogenates of dicentrarchus labrax, was investigated in 5 feeds in relation to their amino acids composition and their ability to sustain growth of d. labrax post larval juveniles. gastric proteolysis was found to contribute <25% of the total proteolytic capacity. correlation was found between intestinal proteolytic capacity and the feed protein content of three pairs of amino acids. the sum of basic amino acids, arginine + lysine in the feed protein ex ... | 1995 | 24197530 |
| circadian rhythms of feeding activity in sea bass, dicentrarchus labrax l.: dual phasing capacity of diel demand-feeding pattern. | the nocturnal versus diurnal feeding patterns of sea bass under controlled experimental conditions were studied in order to investigate the existence of such a dualistic feeding behavior. the animals (six groups of 4 animals and 8 single fish) were held in tanks filled with recirculating salt water and installed in a "chronolab" under constant conditions (23.5 degrees c and 2.4% salinity). the fish were given access to self-demand feeders and first exposed to a photoperiod regime of 12:12 (12 h ... | 1995 | 7488763 |
| unique features in the mitochondrial d-loop region of the european seabass dicentrarchus labrax. | we have cloned and sequenced the displacement-loop (d-loop) region of the mitochondrial dna (mtdna) from the european seabass dicentrarchus labrax (dl). this sequencing revealed the presence of four tandemly repeated elements (r1, r2, r3 and r4); the individual variation in mtdna total length is entirely accounted for by their variable number. the individuals examined also possessed an imperfect copy of one of the tandem repeats (psi r2). at least one termination-associated sequence (tas) is pre ... | 1995 | 7642088 |
| effect of the molecular form of dietary nitrogen supply in sea bass larvae: response of pancreatic enzymes and intestinal peptidases. | sea bass (dicentrarchus labrax) larvae were weaned at day 25 with microparticulated diets in which 10% of the nitrogen supply had different molecular forms: amino acid mixture (slaa), casein hydrolysate (slh) or fish meal (slp). the control group (lp) was fed live prey. no difference was observed in larval growth between the weaned groups, but the survival was significantly higher in the slh group. trypsin secretion was stimulated in the slaa group, whereas the slh diet reduced the secretion fro ... | 1995 | 24197442 |