| genetic variation of border disease virus species strains. | the 5´-untranslated region of pestivirus strains isolated from domestic and wild animals were analysed to determine their taxonomic status according to nucleotide changes in the secondary genomic structure using the palindromic nucleotide substitutions (pns) method. a total of 131 isolates out of 536 pestivirus strains evaluated, were clustered as border disease virus (bdv) species. the bdv strains were further divided into at least 8 genotypes or subspecies. thirty-two isolates from small rumin ... | 2011 | 22194225 |
| implementation of immunohistochemistry on frozen ear notch tissue samples in diagnosis of bovine viral diarrhea virus in persistently infected cattle. | abstract: | 2011 | 22142412 |
| a nested pcr approach for unambiguous typing of pestiviruses infecting cattle. | an atypical pestivirus ('hobi'-like pestivirus, putative bovine viral diarrhoea 3, bvdv-3) was identified firstly in contaminated foetal calf serum batches and isolated subsequently from an outbreak of respiratory disease in a cattle herd in italy. the isolation of the novel pestivirus from animals affected clinically posed concerns about the validity of bvdv eradication programs, considering that 'hobi'-like pestivirus (bvdv-3) is undetected or mistyped by the molecular diagnostic tools current ... | 2011 | 22119481 |
| prevalence study of bovine viral diarrhea virus by evaluation of antigen capture elisa and rt-pcr assay in bovine, ovine, caprine, buffalo and camel aborted fetuses in iran. | abstract: bovine viral diarrhea virus is a pestivirus in the family flaviviridae that cause abortions and stillbirths in livestock and its traditional diagnosis is based on cell culture and virus neutralization test. in this study, for more sensitive, specific detection and determined the prevalence of virus in aborted bovine, ovine, caprine, buffalo and camel fetuses the antigen capture elisa and rt-pcr were recommended. from the total of 2173 aborted fetuses, 347 (15.96%) and 402 (18.49%) were ... | 2011 | 22018096 |
| bovine viral diarrhea virus nonstructural protein 5a is phosphorylated by cdc2 cyclin-dependent kinase in vitro. | bovine viral diarrhea virus (bvdv) is an enveloped, positive-stranded rna virus that belongs to the genus pestivirus within the family flaviviridae. bvdv nonstructural protein 5a (ns5a) is a 56-58-kda phosphorylated zinc metalloprotein and an essential component of the bvdv replicase complex. an earlier report suggested that ns5a is phosphorylated by a serine/threonine protein kinase in bvdv-infected cells; however, the identity of the protein kinase remained unknown. to determine which protein ... | 2011 | 22167250 |
| Inactivation at various temperatures of bovine viral diarrhea virus in beef derived from persistently infected cattle. | Bovine viral diarrhea virus (BVDV) is a pestivirus that is enzootic in most cattle populations throughout the world. This virus is present throughout the body of persistently infected (PI) cattle. Previous research has not assessed the cooking temperature at which BVDV in meat from PI cattle can be inactivated. Therefore, muscle tissue from six PI cattle was harvested, refrigerated, frozen and heated to various internal temperatures. The concentration of virus present was determined by virus iso ... | 2011 | 21965453 |
| detection and identification of the atypical bovine pestiviruses in commercial foetal bovine serum batches. | the recently emerging atypical bovine pestiviruses have been detected in commercial foetal bovine serum (fbs) of mainly south american origin so far. it is unclear how widely the viruses are presented in commercial fbs of different geographic origins. to further investigate the possible pestivirus contamination of commercially available fbs batches, 33 batches of fbs were obtained from ten suppliers and analysed in this study for the presence of both the recognised and the atypical bovine pestiv ... | 2011 | 22174836 |
| a survey of antibodies to pestivirus in sheep in the republic of ireland. | : sera from 1,448 adult ewes in 91 flocks, representing all 26 counties in the republic of ireland, were examined for pestivirus antibodies using a commercially available elisa which detected igg1 antibody to border disease virus. eighty-one sheep (5.6%) in 42 flocks (46.0%) were antibody-positive. within infected flocks, the mean seroprevalence level was 11.4% with a range of 6.3% to 30.0%. the highest antibody prevalence was detected in sheep from central lowland counties of ireland. comparati ... | 2004 | 21851662 |
| Pestivirus and alphaherpesvirus infections in Swedish reindeer (Rangifer tarandus tarandus L.). | Herding semi-domesticated reindeer has economic and social value for Sami people in the northern territories of Fennoscandia. However, with the intensification of reindeer husbandry, interspecies transmission of pathogens between reindeer and domestic animals may become a problem, especially for countries such as Sweden, Norway, and Finland where pestivirus and alphaherpesvirus have been eradicated in domestic ruminants. This study, which included 1158 Swedish reindeer, showed relatively high pr ... | 2011 | 22078277 |
| quinoline tricyclic derivatives. design, synthesis and evaluation of the antiviral activity of three new classes of rna-dependent rna polymerase inhibitors. | in this study three new classes of linear n-tricyclic compounds, derived by condensation of the quinoline nucleus with 1,2,3-triazole, imidazole or pyrazine, were synthesized, obtaining triazolo[4,5-g]quinolines, imidazo[4,5-g]quinolines and pyrido[2,3-g]quinoxalines, respectively. title compounds were tested in cell-based assays for cytotoxicity and antiviral activity against rna viruses representative of the three genera of the flaviviridae family, that is bvdv (pestivirus), yfv (flavivirus) a ... | 2011 | 22047799 |
| hobi-like pestivirus in aborted bovine fetuses. | an outbreak of abortion affecting multiparous cows was associated with hobi-like pestivirus infection. viral rna and antigens were detected in the tissues of two aborted fetuses. molecular assays for other common abortogenic agents tested negative. at the genetic level, the hobi-like pestivirus displayed the closest relatedness to italian, australian, and south american viruses, whereas it diverged from the prototype thai isolate. these findings may have important implications for the pestivirus ... | 2011 | 22162547 |
| a pestivirus dna vaccine based on a non-antibiotic resistance escherichia coli essential gene marker. | antibiotic resistance genes are widely used to produce plasmid dna vaccines, but risk unwanted exposure to antibiotic residues and the spread of resistance genes. to overcome the limitations of existing selection technologies, we developed an alternative system applying the widely used household biocide triclosan as the selective agent and an endogenous growth essential target gene, fabi, as the plasmid-borne marker in escherichia coli. the fabi/triclosan system enables efficient, non-antibiotic ... | 2011 | 22212129 |