| new molecular diagnostic tests for two congenital forms of anemia. | congenital hypoplastic anemias are a rare and heterogeneous group of disorders. this paper reviews new molecular diagnostic tests in two distinct forms of congenital anemias, anemia due to transplacental infection with b19 parvovirus and fanconi anemia. in both instances, molecular assays making use of amplification of dna by the polymerase chain reaction have been used to diagnose either a specific viral infection or gene mutation responsible for a disorder. recognition of these entities has im ... | 1997 | 9021520 |
| polymerase chain reaction amplification of archival material for parvovirus b19 in children with transient erythroblastopenia of childhood. | the relationship between transient erythroblastopenia of childhood (tec) and parvovirus b19 infection remains uncertain. large series using primarily serologic evaluation have not shown an association, whereas smaller series have reported parvovirus b19 infection in such patients. further, parvovirus dna or antigen has been detected in some patients seronegative for the virus at presentation. polymerase chain reaction (pcr) amplification has never been used to evaluate patients with tec for parv ... | 1996 | 9025846 |
| tissue iron storage patterns in fetal hydrops associated with congestive heart failure. | to learn whether fetal congestive heart failure causes a characteristic tissue iron storage pattern, we selected 15 neonatal autopsy cases of hydrops fetalis in which both the clinical and gross autopsy findings suggested intrauterine congestive heart failure. the latter appeared to be due to functional causes in 10 (3 nonhemolytic anemia, 4 cardiac dysrhythmia, 3 dilated cardiomyopathy) and was associated with cardiac malformation in 5. we graded the amount of hepatocellular siderosis, reticulo ... | 1996 | 9025854 |
| diagnostic value of cd45ro expression on circulating t lymphocytes of fetuses and newborn infants with pre-, peri- or early post-natal infections. | we examined the expression of the cd45ro antigen, which characterizes the antigen primed/memory phenotype of t lymphocytes, as a marker for congenital infection in blood samples of newborns and fetuses. cd45ro expression on t cells was determined by triple-colour fluorescence flow cytometry. in total 537 blood samples of newborns and infants up to an age of 3 months and 89 fetal blood samples from gestational weeks 19-31 were analysed. of the newborns and infants, 74 had a clinically, serologica ... | 1997 | 9030868 |
| human parvovirus b19 and blood products. | human b19 parvovirus (b19), identified in 1975, was only recognised as the causative agent of fifth disease in 1983. the incidence of viraemia is low, around 1 in 1,000, but is sufficient to ensure that most plasma pools for fractionation contain some virus. while infection usually occurs in childhood and is benign, chronic infection sometimes occurs and may be of concern in certain patient groups. | 1997 | 9031493 |
| detection of parvovirus b19 in skin biopsy, serum, and bone marrow of a patient with fever, rash, and polyarthritis followed by pneumonia, pericardial effusion, and hepatitis. | a previously healthy 33-year-old male patient presented with fever, rash and polyarthritis. subsequently, he developed pleuropneumonitis, pericardial effusion and hepatitis. the diagnosis of parvovirus b19 infection was based on the detection of parvovirus dna by pcr in a skin biopsy, bone marrow cells and serum. the patient had high parvovirus igg antibody titres but remained negative for igm at a three month follow-up, suggesting persistence of the virus or reinfection. it is concluded that de ... | 1996 | 9031882 |
| parvovirus b19 diagnosis in pregnant women--quantification of igg antibody levels (iu/ml) with reference to the international parvovirus b19 standard serum. | on the basis of the results of a collaborative study the expert committee on biological standardisation of the world health organisation has issued an international standard (is) serum for parvovirus b19 igg antibody (nibsc 93/724). in this study this is was used to calibrate an in-house standard serum for reporting the results of parvovirus b19 igg testing in iu/ml. the igg titre distribution in 939 pregnant women was determined. these samples were sent to the laboratory for determining the imm ... | 1997 | 9039535 |
| [fever, stomatitis, and cutaneous eruption on the hands and feet. "gloves and socks" papular purpuric syndrome]. | | 1996 | 9044647 |
| [evaluation of viral safety of a high-purity human factor viii concentrate submitted to 2 specific virus inactivation treatments (fandhi)]. | to perform a validation study of the production process of a human high purity fviii concentrate, obtained by affinity chromatography and treated with solvent-detergent and 80 degrees c, 72- hour dry heating in the final vial, in order to demonstrate its viral safety. | 1996 | 9045353 |
| parvovirus b19 in human disease. | parvovirus b19, the only known human pathogenic parvovirus, is associated with a wide range of disease manifestations. in healthy individuals, the major presentation of b19 infection is erythema infectiosum. in patients with underlying hemolytic disorders, infection is the primary cause of transient aplastic crisis. in immunosuppressed patients, persistent infection may develop that presents as pure red cell aplasia and chronic anemia. in utero infection may result in hydrops fetalis or congenit ... | 1997 | 9046945 |
| life-threatening anaemia caused by b19 parvovirus infection in a non-immunocompromised patient. | a 21-year-old surinam boy suffering from a combined heterozygous sickle-cell beta-thalassaemia presented with an extreme anaemia (haemoglobin concentration 0.7 mmol/l) and severe haemolysis without signs of increased erythropoiesis. an acute b19 parvovirus infection was diagnosed by a transient increase of igm anti-b19 parvovirus titres and a positive polymerase chain reaction for b19 parvovirus. the patient recovered spontaneously after a short period of supportive care with red cell transfusio ... | 1997 | 9050335 |
| toxoplasmosis and parvovirus b19. | pregnant women might well improve their chances for a successful pregnancy outcome by following the advice of w. c. fields: avoid contact with small children and animals whenever possible. failing widespread acceptance of this philosophy, management of t. gondii and parvovirus b19 infections continues to be a challenge for the foreseeable future. | 1997 | 9067784 |
| [parvovirus b 19 in pregnancy]. | the human parvovirus b 19 was discovered in 1975 by cossart but its etiological association with erythema infectiosum and hydrops of the foetus was proved only in 1984-1985. the objective of the submitted prospective study was to assemble basic findings on the herd immunity of the female population and the risk of infection with this agent during pregnancy. seropositivity of women of fertile age before the epidemic of parvovirus b 19 in 1993 and 1994 was cca 50%, after the epidemic 70%. erythema ... | 1996 | 9072141 |
| parvovirus b19-associated haemophagocytic syndrome with lymphadenopathy resembling histiocytic necrotizing lymphadenitis (kikuchi's disease). | a 15-year-old girl developed a haemophagocytic syndrome caused by human parvovirus b19 (pvb19). the cervical lymph node histology, resembling that of histiocytic necrotizing lymphadenitis (hnl, kikuchi's disease), included several transformed lymphocytes, numerous histocytes, and massive necrosis. we detected pvb19-positive cells in the lymph node by immunohistochemistry. possible autoimmune mechanisms in hnl-like diseases triggered by pvb19 are discussed. | 1997 | 9074434 |
| human parvovirus b19 infection associated with severe acute perimyocarditis in a 34-year-old man. | | 1997 | 9076392 |
| detection of human parvovirus b19 dna in cerebrospinal fluid. | | 1997 | 9076823 |
| virus inactivation of blood products by phenothiazine dyes and light. | methylene blue (mb) and its derivatives azure a, b, c and thionine are photoactive and, in principle, are suitable for photodynamic virus inactivation of blood and blood products, such as therapeutic plasma. methylene blue was selected for plasma decontamination because it is being clinically used and because of its known toxicological and other properties. the standard procedure for photodynamic treatment of single units of fresh plasma involves illumination with visible light at an mb concentr ... | 1997 | 9077128 |
| variability in parvovirus b19 igg levels in intravenous immunoglobulin samples. | | 1996 | 9078448 |
| pancytopenia following infection with human parvovirus b19 as a presenting feature of hereditary spherocytosis in two siblings. | | 1997 | 9079265 |
| parvovirus b19 infection in aids patients. | bone marrow of 61 hiv-1-infected patients and 23 control patients was examined to determine the incidence of b19 infection and its clinical impact in hiv-1-infected persons. of the 61 hiv-infected patients studied, ages ranged from 22-47 years with a mean of 33.2 years. there was a man:woman ratio of 3.8:1. with regard to staging of hiv disease at the time of bone marrow sampling, 52 patients were cdc group 4, 5 patients were cdc group 3, and 4 patients were cdc group 2. control patients, were n ... | 1997 | 9089029 |
| early detection of acute parvovirus b19 infection in hiv disease by dna dot blot hybridization. | | 1997 | 9089034 |
| [acute blood spoliation: an unrecognized hemolytic factor allowing clinical expression of erythroblastopenia caused by parvoviruses b19 in healthy subjects]. | | 1996 | 9092364 |
| [adult-onset still disease associated with acute parvovirus b19 infection in pregnancy]. | | 1996 | 9092365 |
| infection by parvovirus b 19 during pregnancy: a review. | fetal infection by parvovirus b 19 is a common cause of fetal anemia and nonimmune hydrops fetalis and may result in fetal death. recent improvements in diagnosing parvovirus infections by sensitive molecular biology techniques now allow for a new insight into its pathogenic rule, immunology, and the varied clinical manifestations. the estimated overall risk of fetal loss after maternal exposure is about 6.5 percent, which is much less than previously thought. inasmuch as complete spontaneous re ... | 1997 | 9095492 |
| other viral agents of perinatal importance. varicella, parvovirus, respiratory syncytial virus, and enterovirus. | this article summarizes new information on the natural history of maternal varicella and maternal parvovirus b19. controversies in the therapy of respiratory syncytial virus are presented. prophylactic and therapeutic immunotherapy of neonatal enteroviral disease are reviewed. | 1997 | 9099510 |
| late onset of hydrops fetalis following intrauterine parvovirus b19 infection. | we present a case of intrauterine parvovirus b19 infection, diagnosed by detection of b19 dna in the amniotic fluid obtained at 15 weeks of gestation for chromosome analysis in an asymptomatic pregnancy, causing hydrops fetalis 7 weeks later. following intrauterine transfusion, rapid resolution of hydrops could be observed and a healthy boy was born at 38 weeks of gestation. the respective laboratory data obtained from the examination of the amniotic fluid and maternal, fetal, and neonatal blood ... | 1997 | 9101221 |
| microbial dna in the synovium--a role in aetiology or a mere bystander? | | 1997 | 9107236 |
| persistence of parvovirus b19 dna in synovial membranes of young patients with and without chronic arthropathy. | human parvovirus b19 replicates in erythroid precursors of the bone marrow, and several diseases have been attributed to this virus including some cases of juvenile chronic arthropathy. | 1997 | 9107245 |
| reactive arthritis in a patient with simultaneous parvovirus b19 infection and clostridium difficile diarrhoea. | | 1997 | 9117160 |
| evaluation of human parvovirus b19 dna contamination of factor viii products. | | 1996 | 9119140 |
| nosocomial exposure to parvovirus b19: low risk of transmission to healthcare workers. | to evaluate the risk of nosocomial transmission of parvovirus b19 (b19) infection to healthcare workers (hcws) exposed to patients with transient aplastic crisis (tac) caused by acute b19 infection. | 1997 | 9120238 |
| unusual incidence of aplastic anaemia due to b-19 parvovirus infection in renal transplant recipients. | | 1997 | 9123540 |
| a randomised trial of fibrin sealant in peripheral vascular surgery. | in a prospective randomised trial 39 patients undergoing either arterial bypass surgery with a polytetrafluoroethylene (ptfe) bypass graft (n = 18) or aortic aneurysm repair with a woven dacron graft (n = 21) were randomised either to receive fibrin sealant as a topical haemostatic agent at the arterial anastomosis or to act as control. the main outcome measure was the time taken to achieve haemostasis at the suture line. the median time to achieve haemostasis was 0.5 min (range 0-11 min) in the ... | 1996 | 9123925 |
| value of virus filtration as a method for improving the safety of plasma products. | | 1996 | 9123931 |
| establishing the cause of nonimmune hydrops. | | 1997 | 9125628 |
| assessment of the specificity of a commercial human parvovirus b19 igm assay. | it is important to investigate a possible cross-reaction of anti-rubella igm in the ideia parvovirus b19 igm test because many b19 infections are either asymptomatic or have clinical symptoms similar to those of rubella virus infections. epstein-barr virus (ebv) igm, cytomegalovirus (cmv) igm, measles igm and rheumatoid factor (rf) igm cross-reactions were also studied. | 1997 | 9126681 |
| [seroprevalence of b19 parvovirus infection in patients with acute polyarthritis]. | over a period of 29 months, from january 1991 to december 1994, all cases of acute polyarthritis seen at the rheumatology service in our institution were studied to determine the seroprevalence of parvovirus b19 (b19) infection. the variables studied included: age and sex of patients, presence of fever and rash, anti-b19 igm and ige serological determinations (elisa, mardix lab.), follow-up time and final diagnosis. the study included 36 patients (22 women and 14 men, mean age 34 +/- 19 years). ... | 1996 | 9132859 |
| [gloves and socks edema disclosing parvovirus b19 infection]. | | 1997 | 9137404 |
| [cutaneous vasculitis disclosing parvovirus b19 infection]. | | 1997 | 9137705 |
| comparison of seven commercial tests for the detection of parvovirus b19-specific igm. | several enzyme immunoassays (eia) for the detection of parvovirus b19 igm (anti-b19 igm) are now commercially available. in this study, seven commercial eias (biotrin, dako, viramed, viratech, r-biopharm, mast) were compared with an in-house eia (mvp-eia) using native viral b19 particles and the reference igm radioimmunoassay (macria). a total of 88 sera were tested. results agreed in 39/88 (44.3%) sera, whereas 47/88 (53.4%) were discrepant and 2/88 (2.3%) gave an equivocal result. assay sensit ... | 1997 | 9144914 |
| transmission of parvovirus b19 by coagulation factor concentrates exposed to 100 degrees c heat after lyophilization. | double inactivation by solvent/detergent treatment plus heating at 100 degrees c for 30 minutes after lyophilization has been adopted to improve viral safety of factor viii and factor ix concentrates, particularly with respect to non-lipid-enveloped viruses. the aim of this study was to evaluate the safety of concentrates exposed to these virucidal methods. | 1997 | 9149778 |
| postpartum lupus erythematosus associated with parvovirus b19 infection. | we describe postpartum onset of systemic lupus erythematosus (sle) associated with parvovirus b19 infection in a mother presenting with fever, polyarthritis, erythema, and multiorgan involvement. b19 infection was revealed by detection of b19 dna and igm antibodies. in addition, our patient showed low cd4+ (384 x 10(6)/l) and cd8+ (213 x 10(6)/l) t cells, high immunoglobulin values (23.77 g/l), hypocomplementemia, thrombocytopenia, leukopenia, and anemia. her daughter had rash associated with in ... | 1997 | 9150091 |
| experimental infection of cynomolgus monkeys with simian parvovirus. | simian parvovirus is a recently discovered parvovirus that was first isolated from cynomolgus monkeys. it is similar to human b19 parvovirus in terms of virus genome, tropism for erythroid cells, and characteristic pathology in natural infections. cynomolgus monkeys were infected with simian parvovirus to investigate their potential usefulness as an animal model of human b19 parvovirus. six adult female cynomolgus monkeys were inoculated with purified simian parvovirus by the intravenous or intr ... | 1997 | 9151844 |
| parvovirus b19-antibody serosurvey in 500 developmentally disabled subjects. | sera of 500 residents were screened for parvovirus b19 immunoglobulin m (igm) and immunoglobulin g (igg) by enzyme-linked immunosorbent assay. positive igm and equivocal igg or igm results were confirmed by immunofluorescent antibody and western blot. igm was detected in 13 sera (2.6%), and igg was detected in 285 (57%). records of igm-positive residents contained no evidence of erythema infectiosum or polyarthropathy. | 1997 | 9154477 |
| [human parvovirus b19 infection in a child suffering from chronic arthritis]. | the pathogenicity of the human parvovirus b19 has been disclosed in the last two decades. different syndromes (erythema infectiosum or hydrops fetalis) can be produced by this virus as well as aplastic crisis in patients with haemolytic anaemia. it is a common infection both in adults and in children. the frequency of joint symptoms in erythema infectiosum is about 8% in childhood but tenfold of it in the adults. however, the arthropathy could be the only symptom without constitutional complains ... | 1997 | 9157338 |
| quantitation of parvovirus b19 dna sequences by competitive pcr: differential hybridization of the amplicons and immunoenzymatic detection on microplate. | a competitive pcr assay was developed to quantify b19 dna sequences. target and internal standard sequences were co-amplified by the same set of primers. the internal standard competitor was constructed by recombinant pcr and differed from the original genome sequence in a 21-bp mutagenized fragment, internal to the region amplified by the same set of primers. the internal standard competitor was cloned in a plasmid vector and the cloned fragment used in all the experiments. target and internal ... | 1997 | 9160327 |
| [parvovirus b19 in the czech republic. seroepidemiologic study]. | erythema infectiosum which is caused by parvovirus b 19 is notified in the czech republic similarly as some other viral exanthemas under the diagnosis "other viral infections affecting the skin and mucosae, not listed elsewhere" (b08). analysis of the epidemiological position in the czech republic in 1993-1995 was based on epidat data, documentation of epidemic of 1993 in the liberec district and serologically confirmed minor epidemics (1994, 1995). an increased number of cases (in particular in ... | 1997 | 9162451 |
| dot immunoperoxidase assay for detection of parvovirus b19 antigens in serum samples. | we describe a simple and rapid dot immunoperoxidase assay for the direct detection of parvovirus b19 capsid antigens in human sera. the assay was performed with serum specimens dotted onto nylon membranes. vp1 and vp2 b19 antigens, which represent 4 and 96% of the capsid, respectively, were detected with a pool of monoclonal antibodies directed against the two proteins, and the complex was visualized by immunoperoxidase staining. the assay could be performed in about 4 h, and positive results we ... | 1997 | 9163485 |
| parvovirus b19 associated neutropenia. treatment with rh g-csf. | in a 39-year-old patient under follow-up for metastatic adenocarcinoma of the colon, a post-operative fever and a profound neutropenia occurred during hospitalization. a diagnosis of primary infection with parvovirus b-19 was made in view of the association of reticulocytopenia, together with anti-b19 igm antibodies and positive serum results for viral dna. the granulocytic lineage appeared to be normal on bone-marrow smears, and anti pmn autoantibodies were not found. rh g-csf therapy resulted ... | 1997 | 9168305 |
| [molecular biology at the service of the daily medical virology. 2. applications to virological diagnosis]. | molecular biology techniques are applied for the diagnosis of meningoencephalitis due to herpesviruses, enteroviruses or polyomaviruses, for the diagnosis of human cytomegalovirus, human parvovirus b19, varicella-zoster virus and rubella virus infections occurring during pregnancy, for the diagnosis and the management of retrovirus infections (hiv and htlv) and of hepatitis (hbv and hcv), for papillomavirus typing and to detect a link between virus and clinical manifestations (cardiomyopathy or ... | 1997 | 9180961 |
| antibodies to recombinant human erythropoietin causing pure red cell aplasia. | recombinant human erythropoietin (rhuepo) is used extensively in anemic patients on dialysis and other patients and is regarded as very safe and effective in the management of anemia in these patients. to date, there is no report on the development of antibodies to rhuepo in the patients treated with this drug. we report here a patient who developed antibodies to rhuepo and as a result developed pure red cell aplasia. a 63-year-old black male with end-stage renal disease secondary to hypertensio ... | 1997 | 9181281 |
| primary and secondary infections by human parvovirus b19 following bone marrow transplantation: characterization by pcr and b-cell molecular immunology. | due to the preparative regimen necessary, bone marrow transplantation (bmt) consistently results in severe immunodeficiency, often associated with anaemia, leukopenia and thrombocytopenia. parvovirus b19 replicates in red blood cell precursors in the bone marrow and causes erythema infectiosum ('fifth disease'), anaemia, arthritis and foetal death. we assessed the significance of b19 infections as a cause of post-bmt complications. over 900 serial serum samples from 201 allogeneic bone marrow re ... | 1997 | 9181647 |
| [atypical clinical manifestation in a child with parvovirus b19 infection]. | | 1997 | 9183414 |
| viruses and childhood arthritis. | the role of viral infections in the aetiology of acute and chronic arthritides of childhood is incompletely understood. the fact that some viruses cause acute arthritis is certain, although in most instances of presumed viral arthritis no agent is identified. the associations of viruses with diseases such as juvenile chronic arthritis (jca) are limited, and have been difficult to prove with certainty. rubella, parvovirus b19 and influenza ah2n2 have been shown by culture, serology or epidemiolog ... | 1997 | 9187231 |
| persistence of antibody to human parvovirus b19 after allogeneic bone marrow transplantation: role of prior recipient immunity. | human parvovirus b19 (b19) igg was studied retrospectively in 66 allogeneic bone marrow transplantation (bmt) patients using an enzyme-linked immunosorbent assay. recipient and donor sera had been stored pre-bmt together with sequential sera thereafter. approximately half of donors and recipients had anti-b19 igg pre-bmt and thus the relative contributions of donor and recipient immunity to antibody production after transplantation could be assessed. for each patient, a serum taken 2 to 3 years ... | 1997 | 9192791 |
| chronic parvovirus b19 infection resulting in chronic fatigue syndrome: case history and review. | the spectrum of disease caused by parvovirus b19 has been expanding in recent years because of improved and more sensitive methods of detection. there is evidence to suggest that chronic infection occurs in patients who are not detectably immunosuppressed. we report the case of a young woman with recurrent fever and a syndrome indistinguishable from chronic fatigue syndrome. after extensive investigation, we found persistent parvovirus b19 viremia, which was detectable by polymerase chain reacti ... | 1997 | 9195056 |
| chronic anemia as a complication of parvovirus b19 infection in a pediatric kidney transplant patient. | this is a report of unexplained anemia that persisted for 4 months in an adolescent renal transplant patient receiving immunosuppression that included prednisone, tacrolimus, and mycophenolate mofetil. this patient required monthly blood transfusions for fatigue, palpitations, and hematocrit levels between 15% and 17%. in addition, his posttransplant course was notable for the development of insulin-dependent diabetes mellitus. while receiving low-dose prednisone, he was switched from tacrolimus ... | 1997 | 9203192 |
| clinical relevance of parvovirus b19 as a cause of anemia in patients with human immunodeficiency virus infection. | parvovirus b19 (b19) dna was detected by dot blot hybridization in sera from 5 (17%) of 30 human immunodeficiency virus (hiv)-infected patients with hematocrits (hct) of < or =24 and 4 (31%) of 13 hrv-infected patients with hct of < or =20, suggesting that b19 is a reasonably common cause of severe anemia in hiv infection. the anemia promptly remitted after immunoglobulin therapy in 3 of 4 treated patients. the presence of igm to b19, the clinical circumstance in which anemia developed, and the ... | 1997 | 9207380 |
| [a girl with systemic onset juvenile rheumatoid arthritis suspected to be due to human parvovirus b19 infection]. | we encountered a case of a girl where human parvovirus b19 infection was considered to have been concerned with the development of systemic type juvenile rheumatoid arthritis (jra). while the affected child did not show any evident infectious erythema-like findings, changes in the serum antibody titer by the eia method presented the pattern of first infection. during the clinical course the condition of the disease as jra was serious and hemophagocytic syndrome developed concurrently. furthermor ... | 1997 | 9209130 |
| association of parvovirus infection with isolated fetal effusions. | the association of parvovirus b19 infection and hydrops fetalis is well known. however, the association of parvovirus and fetal pleural or pericardial effusions has not been reported. we present five cases of isolated pleural or pericardial effusion with documented maternal parvovirus infection in four of these pregnancies. in the absence of structural or karyotypic abnormalities, spontaneous resolution of the effusion portends for a successful pregnancy outcome. | 1997 | 9217958 |
| nonimmune hydrops fetalis caused by intrauterine human parvovirus b19 infection: a case of spontaneous reversal in utero. | we report a case of spontaneous reversal in utero of hydrops fetalis caused by parvovirus b19 maternal-fetal infection. the route leading to fetal hydrops is not fully understood. severe anemia with hypoxemia and viral fetal myocarditis have been incriminated. then the main issue is fetal death or spontaneous abortion. cases of spontaneous reversal of hydrops fetalis are unusual. fetal regenerative anemia is a good prognostic factor and emphasizes the place of conservative management. | 1997 | 9218942 |
| increased incidence of parvovirus b19. | | 1997 | 9219425 |
| detection of parvovirus b19-specific igm by antibody capture radioimmunoassay. | a solid phase igm-capture radioimmunoassay (macria) for the detection of parvovirus b19-igm is described (cohen et al., 1983, j. hyg. camb. 91, 113-130). igm from a dilution of patients serum is 'captured' onto a solid phase coated by anti-human igm. to determine whether any of the igm is specific for parvovirus b19, b19 antigen is added followed by a detector system. in the macria described here the detector system comprises a mouse monoclonal antibody to parvovirus b19 and a 125i-labelled anti ... | 1997 | 9220384 |
| human parvovirus b19 in bone marrows from adults with acquired immunodeficiency syndrome: a comparative study using in situ hybridization and immunohistochemistry. | human parvovirus b19, which infects and lyses erythroid precursors, can cause severe anemia in patients with immunodeficiency. the incidence of parvovirus infection in adult acquired immunodeficiency syndrome (aids) patients is unknown. eighty-one archival formalin-fixed, paraffin-embedded (ffpe) bone marrow biopsies from 73 aids adults were immunostained with monoclonal r92f6 against b19 vp1 and vp2 capsid proteins using streptavidin peroxidase and streptavidin alkaline phosphatase techniques. ... | 1997 | 9224741 |
| unexpected second trimester pregnancy loss due to maternal parvovirus b19 infection. | five unexplained second trimester fetal deaths occurring in a 3-month interval were evaluated to determine whether the fetal demise was associated with maternal infection due to parvovirus b19. one fetus was markedly hydropic, a classic finding of fetal infection; no gross anomalies were present in the other four. liver specimens from all five fetuses showed viral inclusions by light microscopy, supporting the presence of fetal parvovirus b19 infection. none of the five women had a history of pa ... | 1997 | 9225892 |
| viral arthritis. | viral infections are important in rheumatic disease not only because of the acute and subacute syndromes they cause but also because of their potential importance as etiologic factors in common chronic diseases such as rheumatoid arthritis. during 1996, the clinical spectrum of the acute polyarthritis caused by parvovirus b19 was further delineated and was shown to include carpal tunnel syndrome, hepatic dysfunction, and possibly angioedema. b19 infection can be studied using the salivary antibo ... | 1997 | 9229181 |
| [synchronous variations in the incidence of temporal arteritis and polymyalgia rheumatica in danish counties. association with epidemics of mycoplasma pneumonia infection]. | the incidences of temporal arteritis and polymyalgia rheumatica during a twelve year period were studied in different regions of denmark. data concerning the incidences of these diagnoses were obtained from two general hospitals from 1982 to 1994 and from the national patient register of all diagnoses from all hospitals in 13 of 16 danish counties from 1982 til 1993. data from all temporal artery biopsies in two counties were also obtained. serological epidemiological surveillance data concernin ... | 1997 | 9229871 |
| aplastic anemia due to b19 parvovirus infection in cadaveric renal transplant recipients: an underestimated infectious disease in the immunocompromised host. | parvovirus b19 has been identified as the etiological agent of "fifth disease" in childhood. it is also a rarely reported cause of anemia in transplanted patients. during a period of 18 months we observed four cases (2 male and 2 female; 53 +/- 4.24 years) of severe aplastic anemia due to parvovirus b19 in kidney transplant patients. the overall incidence of the disease was 6.3% of all our transplanted patients. symptoms of the disease occurred 22.5 +/- 9.75 days post-operatively. serum creatini ... | 1997 | 9238623 |
| serum antibodies to parvovirus b19 in patients with pityriasis rosea. | | 1997 | 9252763 |
| zoonotic and viral infection in fetal loss after 12 weeks. | one hundred and thirty-six women from an urban, rural and farming community were recruited to a study of infectious causes of midtrimester miscarriage (n = 85), stillbirth (n = 32), or termination for developmental (n = 17) or chromosomal (n = 2) abnormalities. no woman had evidence of acute infection with toxoplasma, listeria, leptospira or chlamydia psittaci (ovine enzootic abortion). one woman had midtrimester miscarriage associated with primary cytomegolovirus infection and five women had ev ... | 1997 | 9255087 |
| transient erythroblastopenia of childhood associated with human herpesvirus type 6, variant b. | transient erythroblastopenia of childhood (tec) is a disorder of young children ages 3 months to 4 years that is characterized by anemia associated with reticulocytopenia and decreased red blood cell precursors in the bone marrow aspirate (bma). a viral cause has been suspected for tec. bone marrow aspirate-derived dna from 14 children with anemia or another hematologic disorder, including two children with tec, were analyzed by polymerase chain reaction for human herpesvirus type 6 (hhv-6) dna ... | 1997 | 9260752 |
| bacteremia caused by helicobacter cinaedi in an aids patients. | helicobacter cinaedi bacteremia has been infrequently described in homosexual patients with hiv infection. it may recur despite appropriate antimicrobial therapy. we report a bisexual patient with aids in whom h. cinaedi bacteremia developed and presented with prolonged fever and chronic diarrhea. the symptoms resolved without relapse after intravenous immunoglobulin therapy, which was administered for the treatment of concurrent parvovirus b19-associated anemia, and subsequent treatment with cl ... | 1997 | 9262063 |
| pure red cell aplasia due to parvovirus b19 infection in solid organ transplantation. | human parvovirus b19 disease is an infrequent but recognized rare cause of anemia in immunocompromised patients. a few cases of parvovirus b19 infections have been reported in transplant recipients, of those only four patients underwent renal transplantation. the primary immunosuppressive therapy in these patients included prednisone with either cyclosporine or tacrolimus. in one patient the disease was self-limiting, while in three others the hematocrit improved following 10-15 d of treatment w ... | 1997 | 9267713 |
| conjunctivitis associated with fifth disease in a child: a case report. | erythema infectiosum, fifth disease, is a mildly contagious childhood illness that may present with viral conjunctivitis. it is characterized by a facial rash with a "slapped-cheek" appearance and "lacy-like" rash on the trunk and extremities. fifth disease is believed to be caused by parvovirus b19. | 1996 | 9286318 |
| human parvovirus b19 infection: clinical and epidemiological study of 24 cases. | from march 1994 to november 1995 24 cases of human parvovirus b19 infection were seen at the infectious diseases department of the hospital universitário antônio pedro, niterói-rj. serum samples for igm detection (capture enzyme immunoassay) were positive from the 1st to the 27th day after the onset of the exathema. the classical features of erythema infectiosum (slapped cheecked syndrome) were observed in 8 (33.3%) cases all of them children. eight patients (6 adults and 2 children) presented a ... | 1996 | 9293073 |
| parvovirus b19 infections in patients with chronic anemia. | parvovirus b19 has a marked tropism for erythroid progenitor cells and this may lead to chronic anemia in predisposed individuals. it was the purpose of the present study to investigate prospectively the frequency of parvovirus b19 infections in patients with a diagnosis of chronic anemia. | 1997 | 9299850 |
| [severe complications, adverse effects due to blood transfusion and its measures--transfusion transmitted viral infections]. | since the implementation of blood screening for hbs-ag, htlv-1-ab, hiv-ab and hcv-ab, donor blood collected by the japan red cross blood center has become among the safest in the world. the current incidence of hbv, htlv-1, hiv and hcv transmission by blood transfusion are estimated to be 1/500,000, 1/50,000, 1/1,000,000 and 1/500, respectively. even though the latest, most advanced techniques are used, it is impossible to completely prevent transmission of these viruses. hiv transmission from d ... | 1997 | 9301297 |
| human parvovirus b19: immunity of greek females and prenatal investigation of hydrops fetalis. | sera from 308 healthy greek females of reproductive age were examined for specific igg antibodies to human parvovirus b19 by recombinant-elisa to determine the immunity rates of this part of population to parvovirus b19. also paired maternal sera and amniotic fluids from nine pregnancies with hydrops fetalis were investigated for specific igg and igm antibodies and parvovirus dna by polymerase chain reaction and southern hybridization analysis. the latter study was conducted to find out the part ... | 1997 | 9306109 |
| aplastic crisis associated with parvovirus b19 in an adult with hereditary spherocytosis. | parvovirus b19 is usually associated with an acute, self-limited disease in children. in patients with a congenital hemolytic anemia, infection with this virus can cause an aplastic crisis. we describe such a crisis in an adult with asymptomatic hereditary spherocytosis. the association between acute red blood cell aplasia and infection with parvovirus b19 is well described in patients with hereditary hemolytic anemia, particularly sickle cell anemia. this association has also been described, al ... | 1997 | 9308316 |
| chronic anemia due to parvovirus b19 infection in a bone marrow transplant patient after platelet transfusion. | many reports document the transmission of human parvovirus b19 (b19) infection by clotting factor concentrates manufactured from large plasma pools. transmission via other blood components originating from a single donor or a small number of donors, however, seems to occur only rarely. the study reported here identifies a b19 infection that was transmitted via a platelet donation. | 1997 | 9308643 |
| [pcr value in the diagnosis of feto-placental human parvovirus b19 hydrops fetalis: apropos of 10 cases]. | human parvovirus b19 primary infection during pregnancy is responsible for 27% of non autoimmune hydrops fetalis. parvovirus b19 antigen detection and parvovirus b19 igm and igg antibody determination using enzyme immunoassays are not reliable for diagnostic purposes and lack of specificity. parvovirus b19 dna detection in amniotic fluid, fetal blood, ascitic fluid, and fetal biopsies or placenta specimens seems to be the best method for the diagnosis. ninety-seven samples from 70 cases of spont ... | 1997 | 9309232 |
| [human parvovirus b19 infection mimicking systemic lupus erythematosus: case report]. | human parvovirus b19 (hpv-b19) has been known as the etiologic agents of erythema infectiosum in normal childhood, and chronic anemia and thrombocytopenia in immuno-compromised patients. recently, this virus has been reported as the association with rheumatic manifestation such as rheumatoid arthritis and systemic lupus erythematosus (sle). we described here a patient whose hpv-b19 infection was mimiking atypical symptoms of sle at diagnosis, and was persistent because of immuno-suppressive ther ... | 1997 | 9311285 |
| [parvovirus b19 infection in patients with congenital blood coagulation disorders]. | the aim of this study is to assess the prevalence of parvovirus b19 infection in a group of patients affected by congenital coagulation disorders and its association with epidemiological aspects. | 1997 | 9312580 |
| red cell aplasia caused by parvovirus b19 in aids: use of i.v. immunoglobulin. | we describe the case of a 34-year-old man with aids who developed severe anemia due to chronic pure red cell aplasia (prca) caused by parvovirus b19. following initial treatment with an infusion of intravenous immunoglobulin (ivig), 1 g/kg, prca resolved, but there was a recurrence of severe anemia in 3 months. retreatment with 2 g/kg ivig over 2 days resulted in normalization of hemoglobin and a significantly longer remission duration. two doses of 0.4 g/kg ivig q 4 wk failed to prevent a relap ... | 1997 | 9322687 |
| report of a collaborative study to establish the international standard for parvovirus b19 serum igg. | with the introduction of commercial test kits and the wider availability of parvovirus b19 antibody testing, greater standardization is required. moreover, with a b19 vaccine in the early stages of development, there will be long-term need of accurate measurement of parvovirus b19 antibody levels following natural infection and vaccination. a collaborative study was carried out to assess the suitability of a freeze-dried preparation designated 93/724 to serve as the international standard for pa ... | 1997 | 9324996 |
| human parvovirus b19 infection associated with hydrops fetalis. | human parvovirus b19 infection has been associated with fetal anaemia, hydrops and in some cases fetal demise. we report our experience with nonimmune hydrops fetalis due to human parvovirus b19 infection in 17 pregnancies which were identified sonographically over a period of 10 years. the only baby who survived received repeated intrauterine transfusions and was normal neurodevelopmentally at 2 years follow-up. two other fetuses who also received intrauterine transfusion did not survive. all p ... | 1997 | 9325515 |
| acquired pure red cell aplasia in japan. | we reviewed the clinical features of 150 patients with acquired pure red cell aplasia (prca) in japan. there were 35 patients with acute type and 115 with chronic type prca. of the acute prca patients, 17 had human parvovirus b19 infection. drug-induced prca was demonstrated in 7 patients. of the 115 patients with chronic prca, 51 patients were classified as primary and 64 cases were associated with miscellaneous diseases such as thymoma, a variety of hematological disorders and collagen disease ... | 1997 | 9338617 |
| [fetal death in hydrops fetalis at 21 weeks of amenorrhea]. | | 1997 | 9347016 |
| parvovirus [correction of parovirus] b19-induced red cell aplasia in solid-organ transplant recipients. two case reports and review of the literature. | two solid-organ transplant recipients (one heart and one lung) developed severe anemia with reticulocytopenia. both were heavily immunosuppressed. bone marrow aspiration revealed almost complete absence of erythroid precursors. a few giant megaloblastic proerythroblasts with cytoplasmic vacuolisation and intranuclear inclusions were seen. human parvovirus b19 (b19v)-dna genome was found by nested-pcr assays in blood and bone marrow samples in both cases. twelve similar cases are described in the ... | 1997 | 9352328 |
| congenital parvovirus infection. | congenital parvovirus infection was diagnosed in two liveborn premature infants born at 24 and 35 weeks of gestational age. the illnesses were associated with placentomegaly, petechial rash, edema, hepatomegaly, anemia and thrombocytopenia, respiratory insufficiency, and death at 5 and 6 days of age. the syndromes exhibited by these cases shared common but nonspecific features with other life-threatening congenital infections. serological studies in one case supported the diagnosis of parvoviral ... | 1997 | 9353830 |
| anemia and thrombocytopenia due to parvovirus b-19 infection in a pregnant woman. | anemia and thrombocytopenia in a patient with parvovirus b-19 and hepatitis c infection is reported. a seven month-pregnant 20 year-old patient had been first found to be anemic and thrombocytopenic 40 days before admission to our hospital and she had been given methylprednisolone and red cell transfusions. she gave birth to a healthy baby after only eight months of pregnancy. ten days after delivery she was admitted to our hospital because of anemia and thrombocytopenia which did not respond to ... | 1997 | 9355028 |
| influence of maternal infections with viral agents or toxoplasma gondii during pregnancy on fetal ige production. | the importance of maternal infections with toxoplasma gondii, cytomegalovirus (cmv), parvovirus b19, respiratory syncytial virus (rsv), and influenza a and b on fetal ige synthesis was studied in 153 pregnant women. no case of specific igm activity or viral dna in cord blood, indicating a congenital infection, was found. from gestational week 15 to delivery, maternal igg-ab seroconversion to parvovirus b19, rsv, influenza a, or influenza b occurred in 47 women. at delivery, serologic signs of pa ... | 1997 | 9360748 |
| evidence for persistence of human parvovirus b19 dna in bone marrow. | a nested polymerase chain reaction assay (npcr) was used to investigate the potential of human parvovirus b19 dna to persist in blood or bone marrow samples obtained either from blood donors or cadaveric bone donors or from patients presenting with clinical signs of parvovirus b19 infection. the presence of parvovirus b19 specific antibody in blood was tested by enzyme immunoassay (eia). b19 virus genome was not detected in any blood sample of 115 blood donors, of whom 92 (80%) had anti-b19 igg ... | 1997 | 9365887 |
| possible transmission of parvovirus b19 from intravenous immune globulin. | to look for genetic changes in human parvovirus b19 that might be associated with chronic infection, we sequenced b19 dna obtained from serum specimens collected over an approximately 1-year period from a patient with systemic vasculitis. a comparison of the nucleotide sequences of the vp1/vp2 gene from four specimens revealed an abrupt change in the b19 genotype that coincided with initiation of intravenous immune globulin (ivig) therapy. we suspect that one or more of the lots of ivig administ ... | 1997 | 9365888 |
| the 3' untranslated region of the b19 parvovirus capsid protein mrnas inhibits its own mrna translation in nonpermissive cells. | although parvoviruses are found throughout the animal kingdom, only the human pathogenic b19 virus has so far been shown to possess a limited host range, with erythroid progenitor cells as the main target cells supporting b19 propagation. the underlying mechanism of such erythroid tropism is still unexplained. synthesis of the ns1 nonstructural protein occurs in permissive and nonpermissive cells, such as megakaryocytes, whereas synthesis of the vp1 and vp2 capsid proteins seems to be restricted ... | 1997 | 9371610 |
| human parvovirus b19 infection. | human parvovirus b19 shows remarkable tropism for human erythroid progenitor cells. the pathogenesis of disease resulting from infection is a dynamic between the viral suppression of erythropoiesis and the host's ability to mount an effective immune response. understanding this process has led to strategies for treating chronic anemia in persistently infected patients and may result in the development of a vaccine to prevent infection. | 1996 | 9372060 |
| [torch congenital infections]. | | 1997 | 9382270 |
| human parvovirus b19 infection associated with idiopathic thrombocytopenic purpura in a child following liver transplantation. | idiopathic (autoimmune) thrombocytopenic purpura has been previously reported as a rare complication in children following parvovirus b19 infection. in the immunocompromised host who is unable to produce neutralizing antibody, an infection with parvovirus b19 can persist and cause chronic bone marrow failure. | 1997 | 9382984 |
| perinatal parvovirus b19 infections: what are the clinical consequences? | | 1997 | 9383813 |
| pure red blood cell aplasia during azathioprine therapy associated with parvovirus b19 infection. | | 1997 | 9384349 |