| the dahlia mosaic virus gene vi product n-terminal region is involved in self-association. | the genome of the floriculture pathogen dahlia mosaic caulimovirus (dmv) encodes six open reading frames. generally, caulimovirus gene vi products (p6s) are thought to be multifunctional proteins required for viral infection and it is likely that self-association is required for some of these functions. in this study, yeast two-hybrid and maltose binding protein (mbp) pull-down assays indicated that full-length dmv p6 specifically self-associates. further analyses indicated that only the dmv p6 ... | 2011 | 21571015 |
| overexpression of cinnamate 4-hydroxylase gene enhances biosynthesis of decursinol angelate in angelica gigas hairy roots. | angelica gigas is a medicinal plant that produces pyranocoumarins, including decursin (d) and decursinol angelate (da), which have neuroprotective, anticancer, and antiandrogenic effects. in this study, the coumarin biosynthetic pathway was engineered to increase the production of da. specifically, a vector was constructed which contained the a. gigas phenylalanine ammonia-lyase (agpal) and cinnamate 4-hydroxylase (agc4h) genes that were driven by the cauliflower mosaic virus (camv) 35s promoter ... | 2011 | 21626264 |
| agrobacterium-mediated infection of whole plants by yellow dwarf viruses. | barley yellow dwarf virus-pav (bydv-pav) and cereal yellow dwarf virus-rpv (cydv-rpv) are only transmitted between host plants by aphid vectors and not by mechanical transmission. this presents a severe limitation for the use of a reverse genetics approach to analyze the effects of mutations in these viruses on plant infection and aphid transmission. here we describe the use of agroinfection to infect plants with bydv-pav and cydv-rpv. the cdnas corresponding to the complete rna genomes of bydv- ... | 2011 | 21763366 |
| overexpression of a resveratrol synthase gene (pcrs) from polygonum cuspidatum in transgenic arabidopsis causes the accumulation of trans-piceid with antifungal activity. | although resveratrol-forming stilbene synthase (sts) genes have been well characterized in many plant species, there are only a few descriptions about sts genes from polygonum cuspidatum sieb. et zucc, an important medicinal crop in asian countries. to evaluate the biological functions of a polygonum cuspidatum resveratrol synthase gene (pcrs), the pcrs gene was expressed in arabidopsis under the control of cauliflower mosaic virus (camv) 35s promoter. integration and expression of transgene in ... | 2011 | 21717185 |
| the purine-rich dna binding protein ospur╬▒ participates in the regulation of the rice sucrose synthase 1 gene expression. | the rice sucrose synthase 1 (rsus1) gene is transcriptionally induced by sucrose, and a region within its promoter, at -1117 to -958 upstream of the transcription initiation site, was found to be essential for enhancing the sucrose-induced expression. further dissection of this region revealed that a group of nuclear proteins interact with a 39-bp fragment named a-3-2 (-1045 to -1007). a protein that specifically and directly interacted with a-3-2 was isolated from the suspension-cultured cells ... | 2011 | 21834856 |
| analysis of the enhancer-blocking function of the tbs element from petunia hybrida in transgenic arabidopsis thaliana and nicotiana tabacum. | transcriptional enhancers possess the ability to override the tissue-specificity and efficiency of nearby promoters, which is of concern when generating transgenic constructs bearing multiple cassettes. one means of preventing these inappropriate interactions is through the use of enhancer-blocking insulators. the 2-kb transformation booster sequence (tbs) from petunia hybrida has been shown previously to exhibit this function when inserted between an enhancer and promoter in transgenic arabidop ... | 2011 | 21706298 |
| An efficient Agrobacterium-mediated transformation and regeneration system for leaf explants of two elite aspen hybrid clones Populus alba-á+ù-áP. berolinensis and Populus davidiana-á+ù-áP. bolleana. | Transgenic technology has been successfully used for gene function analyses and trait improvement in cereal plants. However, its usage is limited in woody plants, especially in the difficult-to-transform but commercially viable hybrid poplar. In this work, an efficient regeneration and transformation system was established for the production of two hybrid aspen clones: Populus alba-á+ù-áP. berolinensis and Populus davidiana-á+ù-áP. bolleana. A plant transformation vector designed to express the ... | 2011 | 21717184 |
| a role for phantastica in medio-lateral regulation of adaxial domain development in tomato and tobacco leaves. | background and aimsdiverse leaf forms in nature can be categorized into two groups: simple and compound. a simple leaf has a single blade unit, whilst a compound leaf is dissected into leaflets. for both simple and compound leaves, a myb domain transcription factor phantastica (phan) plays an important role in establishing the adaxial domain in the leaf. absence of phan in arabidopsis and antirrhinum leaves supresses blade development, and in tomato suppresses leaflet development. however, in th ... | 2011 | 22184618 |
| Robust RNAi-based resistance to mixed infection of three viruses in soybean plants expressing separate short hairpins from a single transgene. | Transgenic plants expressing double-stranded RNA (dsRNA) of virus origin have been previously shown to confer resistance to virus infections through the highly conserved RNA-targeting process termed RNA silencing or RNA interference (RNAi). In this study we applied this strategy to soybean plants and achieved robust resistance to multiple viruses with a single dsRNA-expressing transgene. Unlike previous reports that relied on the expression of one long inverted repeat (IR) combining sequences of ... | 2011 | 21999157 |
| Post-synthetic modification of plant cell walls by expression of microbial hydrolases in the apoplast. | The systematic creation of defined cell wall modifications in the model plant Arabidopsis thaliana by expression of microbial hydrolases with known specific activities is a promising approach to examine the impacts of cell wall composition and structure on both plant fitness and cell wall recalcitrance. Moreover, this approach allows the direct evaluation in living plants of hydrolase specificity, which can differ from in vitro specificity. To express genes encoding microbial hydrolases in A. th ... | 2011 | 21910026 |
| differences in the mechanism of inoculation between a semipersistent and a non-persistent aphid-transmitted plant virus. | inoculation of the semipersistent cauliflower mosaic virus (camv, genus caulimovirus) is associated to successive brief (5-10 s) intracellular stylet punctures (pd) when aphids probe in epidermal and mesophyll cells. in contrast to non-persistent viruses, there is no evidence of which of the pd sub-phases (ii-1, ii-2 and ii-3) is involved in the inoculation of camv. experiments were conducted using the electrical penetration graph (epg) technique to investigate what particular sub-phases of the ... | 2011 | 22090212 |
| Arabidopsis RGLG2, functioning as a RING E3 ligase, interacts with AtERF53 and negatively regulates the plant drought stress response. | Transcriptional activities of plants play important roles in responses to environmental stresses. AtERF53 is a drought-induced transcription factor, which belongs to the AP2/ERF superfamily, and has a highly conserved AP2 domain. It can regulate drought-responsive gene expressions by binding to the GCC box and/or dehydration-responsive element (DRE) in the promoter of downstream genes. Overexpression of AtERF53 driven by the CaMV35S promoter resulted in an unstable drought-tolerant phenotype in ... | 2011 | 22095047 |
| high level expression of acidothermus cellulolyticus beta-1, 4-endoglucanase in transgenic rice enhances the hydrolysis of its straw by cultured cow gastric fluid. | abstract: background: large-scale production of effective cellulose hydrolytic enzymes is the key to bioconversion of agricultural residues to ethanol. the goal of this study was to develop rice plant as a bioreactor for large-scale production of cellulose hydrolytic enzymes via genetic transformation and to improve rice straw simultaneously as an efficient biomass feedstock for conversion of cellulose to glucose. results: in this study, the cellulose hydrolytic enzyme beta-1, 4-endoglucanase ( ... | 2011 | 22152050 |
| expressing scacr3 in rice enhanced arsenite efflux and reduced arsenic accumulation in rice grains. | arsenic (as) accumulation in rice grain poses a serious health risk to populations with high rice consumption. extrusion of arsenite [as(iii)] by scacr3p is the major arsenic detoxification mechanism in saccharomyces cerevisiae. however, scacr3p homolog is absent in higher plants, including rice. in this study, scacr3 was introduced into rice and expressed under the control of the cauliflower mosaic virus (camv) 35s promoter. in the transgenic lines, as concentrations in shoots and roots were ab ... | 2011 | 22107880 |
| top 10 plant viruses in molecular plant pathology. | many scientists, if not all, feel that their particular plant virus should appear in any list of the most important plant viruses. however, to our knowledge, no such list exists. the aim of this review was to survey all plant virologists with an association with molecular plant pathology and ask them to nominate which plant viruses they would place in a 'top 10' based on scientific/economic importance. the survey generated more than 250 votes from the international community, and allowed the gen ... | 2011 | 22017770 |
| vapa, an innovative "virus-acquisition phenotyping assay" opens new horizons in research into the vector-transmission of plant viruses. | host-to-host transmission--a key step in plant virus infection cycles--is ensured predominantly by vectors, especially aphids and related insects. a deeper understanding of the mechanisms of virus acquisition, which is critical to vector-transmission, might help to design future virus control strategies, because any newly discovered molecular or cellular process is a potential target for hampering viral spread within host populations. with this aim in mind, an aphid membrane-feeding assay was de ... | 2011 | 21853093 |
| identification of seed dormancy mutants by activation tagging. | activation tagging is an important tool for gene discovery in plants. this method utilizes a t-dna sequence that contains four tandem copies of the cauliflower mosaic virus 35s enhancer sequence or promoters oriented outward to the t-dna border sequences. these elements enhance the expression of genes neighboring on either side of the randomly integrated t-dna, resulting in gain-of-function phenotypes. activation tagging has identified a number of genes, including those fundamental to plant deve ... | 2011 | 21898257 |
| Overexpression of a cotton cyclophilin gene (GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pv. tabaci infection. | The full-length cDNA of a cyclophilin-like gene was cloned from Gossypium hirsutum using rapid amplification of cDNA ends and was designated as GhCyp1, a member of the immunophilin protein family. GhCyp1 expression level was higher in roots and stems than in other tissues of cotton, as determined by real-time reverse transcription polymerase chain reaction (RT-PCR). To characterize the GhCyp1 gene, tobacco (Nicotiana tabacum) was transformed via Agrobacterium tumefaciens with a vector to express ... | 2011 | 22000049 |
| enhancing transgenic pea (pisum sativum l.) resistance against fungal diseases through stacking of two antifungal genes (chitinase and glucanase). | one way of enhancing and broadening resistance of plants to different biotic and abiotic stresses is to combine transgenes expressing several genes into a single line. this can be done using different strategies such as crossing, single vector with multiple genes, co-transformation, sequential transformation and ires elements. in the present study conventional crossing method was used. parental transgenic lines transformed via agrobacterium tumefasciens-mediated gene transformation with pgreenii ... | 2011 | 21971070 |
| immunogenicity of foot-and-mouth disease virus structural polyprotein p1 expressed in transgenic rice. | transgenic plants have become developed as bioreactors for producing heterologous proteins and may even form edible vaccines. in the present study, a transgenic rice expressing the capsid precursor polypeptide (p1) gene of foot-and-mouth disease virus (fmdv), under the control of a dual cauliflower mosaic virus (camv 35s) promoter, was generated by agrobacterium-mediated transformation. southern blot, northern blot, western blot, and elisa analyses confirmed that the p1 gene was integrated into ... | 2012 | 22274594 |
| transient expression of cor gene in papaver somniferum. | papaver somniferum is the commercial source of morphine and codeine. the isolation of effective genes involved in the morphine biosynthesis of p. somniferum is very important in the production of specific metabolites achieved using metabolic engi-neering techniques. in this pathway, the key enzyme cor is involved in the conversion of codeinone to codeine and morphinone to morphine. | 2011 | 23678433 |
| qualitative and event-specific real-time pcr detection methods for bt brinjal event ee-1. | bt brinjal event ee-1 with cry1ac gene, expressing insecticidal protein against fruit and shoot borer, is the first genetically modified food crop in the pipeline for commercialization in india. qualitative polymerase chain reaction (pcr) along with event-specific conventional as well as real-time pcr methods to characterize the event ee-1 is reported. a multiplex (pentaplex) pcr system simultaneously amplifying cry1ac transgene, cauliflower mosaic virus (camv) 35s promoter, nopaline synthase (n ... | 2012 | 23451391 |
| a new strategy to improve a cauliflower mosaic virus vector. | co-infection of plants with non-overlapping deletion mutants of cauliflower mosaic virus usually leads to the production of the wild-type virus. to prevent this, a pair of mutants with overlapping deletions was constructed. in infected plants both mutant dnas were stably maintained. such mutants with overlapping deletions will be used as a vector to overcome the size limitation of genes to be cloned. | 2007 | 1937020 |
| expression of inducible angiosperm promoters in a gymnosperm, picea glauca (white spruce). | electrical discharge particle acceleration was used to test the transient expression of numerous inducible angiosperm promoters in a gymnosperm picea glauca (white spruce). promoter expression was assayed in three different tissues capable of in vitro regeneration, zygotic embryos, seedlings and embryogenic callus. the promoters tested include the light-inducible arabidopsis and soybean ribulose-1,5-bisphosphate small subunit promoters and a maize phosphoenolpyruvate carboxylase promoter; a soyb ... | 2003 | 1868222 |
| dissection of 5' upstream sequences for selective expression of the nicotiana plumbaginifolia rbcs-8b gene. | we have previously isolated and characterized a gene (rbcs-8b) from the wild-type species nicotiana plumbaginifolia, encoding the small subunit of ribulose-1,5-bisphosphate carboxylase. using transgenic n. plumbaginifolia as a host, we found that a 5' upstream region (-1038 to +32) of rbcs-8b contains all the sequences required for organ-specific and light-dependent expression of the gene. here we report a detailed analysis of the 5' upstream region of rbcs-8b. gene transfer experiments indicate ... | 2011 | 3226423 |
| the structure of cauliflower mosaic virus. i. ph-induced structural changes. | | 1998 | 34923 |
| correlation of the expression of the nuclear photosynthetic gene st-ls1 with the presence of chloroplasts. | a detailed analysis of the expression of a chimeric gene, consisting of the upstream region of the nuclear photosynthetic gene st-ls1, encoding a component of the water-oxidizing complex of photosystem ii, fused to the coding sequence of beta-glucuronidase (gus) as a reporter, is described. the expression of this chimeric gene at the cellular level was detected by histochemical methods and shows that the expression of this gene is correlated with the presence of chloroplasts. interestingly, the ... | 2006 | 2583121 |
| proximity to the promoter inhibits recognition of cauliflower mosaic virus polyadenylation signal. | the replication of retroviruses and pararetroviruses (such as caulimo- and hepadnaviruses) involves the production of terminally redundant genomic-length rna. the sequence repeated at both ends of the rna (the r region) contains a polyadenylation signal, and for production of full-length rna the version of this at the 5' end of the template must be bypassed by rna polymerase, but the version at the 3' end must be recognized. this implies that the position of the polyadenylation signal determines ... | 2008 | 2366867 |
| cell wall protrusions induced by cauliflower mosaic virus in chinese cabbage leaves: a cytochemical and autoradiographic study. | | 2014 | 4135909 |
| point mutations in cauliflower mosaic virus gene vi confer host-specific symptom changes. | mutants of cauliflower mosaic virus (camv) strain d4 have been characterized with regard to host-specific phenotypes that resulted from specific changes in the viral dna sequence. both the mutant and the wild-type viruses infect a brassicaceous host, brassica campestris, systemically, giving indistinguishable symptoms. however, in the solanaceous host datura stramonium, which was systemically infectible by the wild-type virus, mutants induced necrotic local lesions at 21 degrees c and above, and ... | 2007 | 2134858 |
| gene i products of cauliflower mosaic virus detected in extracts of infected tissue. | the product of cauliflower mosaic virus (camv) gene i has been characterized from extracts of infected plants. two size classes of this protein can be identified by the use of specific antiserum. the antiserum was induced against a chimeric protein produced in e. coli from a gene fusion between a fragment of the camv genome and the beta-galactosidase gene. a 36-kda form of the gene i product is associated with virus particles. a 45-kda form of this product was found only in the insoluble fractio ... | 2013 | 3590626 |
| recombinase polymerase amplification (rpa) of camv-35s promoter and nos terminator for rapid detection of genetically modified crops. | recombinase polymerase amplification (rpa) is a novel isothermal dna amplification and detection technology that enables the amplification of dna within 30 min at a constant temperature of 37-42 °c by simulating in vivo dna recombination. in this study, based on the regulatory sequence of the cauliflower mosaic virus 35s (camv-35s) promoter and the agrobacterium tumefaciens nopaline synthase gene (nos) terminator, which are widely incorporated in genetically modified (gm) crops, we designed two ... | 2014 | 25310647 |
| induction of salt and osmotic stress tolerance by overexpression of an intracellular vesicle trafficking protein atrab7 (atrabg3e). | adaptation to stress requires removal of existing molecules from various cellular compartments and replacing them with new ones. the transport of materials to and from the specific compartments involved in the recycling and deposition of macromolecules is carried out by an intracellular vesicle trafficking system. here, we report the isolation of a vesicle trafficking-regulating gene, atrabg3e (formerly atrab7), from arabidopsis. the gene was induced during programmed cell death after treatment ... | 2004 | 14657401 |
| cauliflower mosaic virus, a compatible pathogen of arabidopsis, engages three distinct defense-signaling pathways and activates rapid systemic generation of reactive oxygen species. | we analyzed expression of marker genes for three defense pathways during infection by cauliflower mosaic virus (camv), a compatible pathogen of arabidopsis (arabidopsis thaliana), using luciferase reporter transgenes and directly by measuring transcript abundance. expression of pr-1, a marker for salicylic acid signaling, was very low until 8 d postinoculation and then rose sharply, coinciding with the rise in virus levels. in contrast, as early as 2 h postinoculation, transcriptional up-regulat ... | 2005 | 16169957 |
| pathogen phytosensing: plants to report plant pathogens. | real-time systems that provide evidence of pathogen contamination in crops can be an important new line of early defense in agricultural centers. plants possess defense mechanisms to protect against pathogen attack. inducible plant defense is controlled by signal transduction pathways, inducible promoters and cis-regulatory elements corresponding to key genes involved in defense, and pathogen-specific responses. identified inducible promoters and cis-acting elements could be utilized in plant se ... | 2008 | 27879840 |
| over-expression of a tomato n-acetyl-l-glutamate synthase gene (slnags1) in arabidopsis thaliana results in high ornithine levels and increased tolerance in salt and drought stresses. | a single copy of the n-acetyl-l-glutamate synthase gene (slnags1) has been isolated from tomato. the deduced amino acid sequence consists of 604 amino acids and shows a high level of similarity to the predicted arabidopsis nags1 and nags2 proteins. furthermore, the n-terminus argb domain and the c-terminus arga domain found in slnags1 are similar to the structural arrangements that have been reported for other predicted nags proteins. slnags1 was expressed at high levels in all aerial organs, an ... | 2009 | 19357433 |
| cauliflower mosaic virus protein p6 inhibits signaling responses to salicylic acid and regulates innate immunity. | cauliflower mosaic virus (camv) encodes a multifunctional protein p6 that is required for translation of the 35s rna and also acts as a suppressor of rna silencing. here we demonstrate that p6 additionally acts as a pathogenicity effector of an unique and novel type, modifying npr1 (a key regulator of salicylic acid (sa)- and jasmonic acid (ja)-dependent signaling) and inhibiting sa-dependent defence responses we find that that transgene-mediated expression of p6 in arabidopsis and transient exp ... | 2012 | 23071821 |
| overexpression of atalmt1 in the arabidopsis thaliana ecotype columbia results in enhanced al-activated malate excretion and beneficial bacterium recruitment. | atalmt1 (arabidopsis thaliana aluminum activated malate transporter 1) encodes an arabidopsis thaliana malate transporter that has a pleiotropic role in arabidopsis stress tolerance. malate released through atalmt1 protects the root tip from al rhizotoxicity, and recruits beneficial rhizobacteria that induce plant immunity. to examine whether the overexpression of atalmt1 can improve these traits, the gene, driven by the cauliflower mosaic virus 35s promoter, was introduced into the arabidopsis ... | 2013 | 23857348 |
| a vamp-associated protein, pva31 is involved in leaf senescence in arabidopsis. | vamp-associated proteins (vaps) are highly conserved among eukaryotes. here, we report a functional analysis of one of the vaps, pva31, and demonstrate its novel function on leaf senescence in arabidopsis. the expression of pva31 is highly induced in senescence leaves, and localizes to the plasma membrane as well as the ara7-positive endosomes. yeast two-hybrid analysis demonstrates that pva31 is interacted with the plasma membrane localized-vamp proteins, vamp721/722/724 but not with the endoso ... | 2015 | 25897470 |
| enhanced resistance to bacterial and fungal pathogens by overexpression of a human cathelicidin antimicrobial peptide (hcap18/ll-37) in chinese cabbage. | the human cathelicidin antimicrobial protein hcap18, which includes the c-terminal peptide ll-37, is a multifunctional protein. as a possible approach to enhancing the resistance to plant disease, a dna fragment coding for hcap18/ll-37 was fused at the c-terminal end of the leader sequence of endopolygalacturonase-inhibiting protein under the control of the cauliflower mosaic virus 35s promoter region. the construct was then introduced into brassica rapa. ll-37 expression was confirmed in transg ... | 2011 | 22308171 |
| genetic manipulation of alcohol dehydrogenase levels in ripening tomato fruit affects the balance of some flavor aldehydes and alcohols | tomato (lycopersicon esculentum) plants were transformed with gene constructs containing a tomato alcohol dehydrogenase (adh) cdna (adh 2) coupled in a sense orientation with either the constitutive cauliflower mosaic virus 35s promoter or the fruit-specific tomato polygalacturonase promoter. ripening fruit from plants transformed with the constitutively expressed transgene(s) had a range of adh activities; some plants had no detectable activity, whereas others had significantly higher adh activ ... | 1998 | 9662548 |
| lignin monomer composition is determined by the expression of a cytochrome p450-dependent monooxygenase in arabidopsis. | the phenylpropanoid pathway provides precursors for the biosynthesis of soluble secondary metabolites and lignin in plants. ferulate-5-hydroxylase (f5h) catalyzes an irreversible hydroxylation step in this pathway that diverts ferulic acid away from guaiacyl lignin biosynthesis and toward sinapic acid and syringyl lignin. this fact led us to postulate that f5h was a potential regulatory step in the determination of lignin monomer composition. to test this hypothesis, we have used arabidopsis to ... | 1998 | 9618461 |
| decreased cell wall digestibility in canola transformed with chimeric tyrosine decarboxylase genes from opium poppy | tyrosine decarboxylase (tydc) is a common plant enzyme involved in the biosynthesis of numerous secondary metabolites, including hydroxycinnamic acid amides. although a definite function has not yet been determined, amides have been proposed to form a physical barrier against pathogens because they are usually found as integral cell wall components. canola (brassica napus) was independently transformed with chimeric genes (35s::tydc1 and 35s::tydc2) under the transcriptional control of the cauli ... | 1999 | 10398700 |
| overexpression of a novel arabidopsis gene related to putative zinc-transporter genes from animals can lead to enhanced zinc resistance and accumulation. | we describe the isolation of an arabidopsis gene that is closely related to the animal znt genes (zn transporter). the protein encoded by the zat (zn transporter of arabidopsis thaliana) gene has 398 amino acid residues and is predicted to have six membrane-spanning domains. to obtain evidence for the postulated function of the arabidopsis gene, transgenic plants with the zat coding sequence under control of the cauliflower mosaic virus 35s promoter were analyzed. plants obtained with zat in the ... | 1999 | 10069843 |
| overexpression of a gene encoding a cytochrome p450, cyp78a9, induces large and seedless fruit in arabidopsis. | an activation tagging screen in which the cauliflower mosaic virus 35s enhancer was inserted randomly into an arabidopsis genome homozygous for the floral homeotic mutation apetala2-1 (ap2-1) resulted in a line (28-5) with extraordinarily wide, heart-shaped ovaries. the ovary of the 28-5 ap2-1 mutant shows an oval shape because of increased numbers of enlarged cells. when the ap2-1 mutation is crossed out of the genetic background, more elongated rather than wider fruits are obtained. normally, ... | 2000 | 11006330 |
| identification of a cyp84 family of cytochrome p450-dependent mono-oxygenase genes in brassica napus and perturbation of their expression for engineering sinapine reduction in the seeds. | cyp84 is a recently identified family of cytochrome p450-dependent mono-oxygenases defined by a putative ferulate-5-hydroxylase (f5h) from arabidopsis. until recently f5h has been thought to catalyze the hydroxylation of ferulate to 5-oh ferulate en route to sinapic acid. sinapine, a sinapate-derived ester in the seeds, is antinutritional and a target for elimination in canola meal. we have isolated three f5h-like genes (bnf5h1-3) from a cultivated brassica napus, whose amphidiploid progenitor i ... | 2000 | 10938378 |
| iron-superoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance. | to determine whether overexpression of fe-superoxide (sod) dismutase would increase superoxide-scavenging capacity and thereby improve the winter survival of transgenic alfalfa (medicago sativa l.) plants, two genotypes were transformed with the vector pexsod10, which contains a cdna for arabidopsis fe-sod with a chloroplast transit peptide and cauliflower mosaic virus 35s promoter. a novel fe-sod was detected by native page in both greenhouse- and field-grown transgenic plants, but activity var ... | 2000 | 10759538 |
| five geranylgeranyl diphosphate synthases expressed in different organs are localized into three subcellular compartments in arabidopsis. | geranylgeranyl diphosphate (ggpp) is the precursor for the biosynthesis of gibberellins, carotenoids, chlorophylls, isoprenoid quinones, and geranylgeranylated proteins in plants. there is a small gene family for ggpp synthases encoding five isozymes and one related protein in arabidopsis, and all homologs have a putative localization signal to translocate into specific subcellular compartments. using a synthetic green fluorescent protein (sgfp), we studied the subcellular localization of these ... | 2000 | 10759500 |
| antisense hema1 rna expression inhibits heme and chlorophyll biosynthesis in arabidopsis. | 5-aminolevulinic acid (ala) is a precursor in the biosynthesis of tetrapyrroles including chlorophylls and heme. the formation of ala involves two enzymatic steps which take place in the chloroplast in plants. the first enzyme, glutamyl-trna reductase, and the second enzyme, glutamate-1-semialdehyde-2,1-aminomutase, are encoded by the nuclear hema and gsa genes, respectively. to assess the significance of the hema gene for chlorophyll and heme synthesis, transgenic arabidopsis plants that expres ... | 2000 | 10631248 |
| the arabidopsis somatic embryogenesis receptor kinase 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. | we report here the isolation of the arabidopsis somatic embryogenesis receptor-like kinase 1 (atserk1) gene and we demonstrate its role during establishment of somatic embryogenesis in culture. the atserk1 gene is highly expressed during embryogenic cell formation in culture and during early embryogenesis. the atserk1 gene is first expressed in planta during megasporogenesis in the nucellus [corrected] of developing ovules, in the functional megaspore, and in all cells of the embryo sac up to fe ... | 2001 | 11706164 |
| antisense inhibition of threonine synthase leads to high methionine content in transgenic potato plants. | methionine (met) and threonine (thr) are members of the aspartate family of amino acids. in plants, their biosynthetic pathways diverge at the level of o-phosphohomo-serine (ser). the enzymes cystathionine gamma-synthase and thr synthase (ts) compete for the common substrate o-phosphohomo-ser with the notable feature that plant ts is activated through s-adenosyl-met, a metabolite derived from met. to investigate the regulation of this branch point, we engineered ts antisense potato (solanum tube ... | 2001 | 11706163 |
| overexpression of acyl carrier protein-1 alters fatty acid composition of leaf tissue in arabidopsis. | acyl carrier protein (acp) is a small (9 kd) acidic protein that is an essential cofactor in plant fatty acid biosynthesis. most plants have several isoforms of acp, some of which are expressed constitutively and others that appear to be more tissue specific. although the critical role of acp in fatty acid biosynthesis has been established, the role of the diverse number of isoforms has yet to be elucidated. we have generated transgenic arabidopsis plants that express high levels of acp-1, a see ... | 2001 | 11553750 |
| somatic and germinal excision activities of the arabidopsis transposon tag1 are controlled by distinct regulatory sequences within tag1. | various sequences within tag1, the endogenous transposon of arabidopsis, were examined to determine how tag1 excision and expression are regulated. the 5' intron for the major 2.3-kb tag1 transcript was found to be critical for the accumulation of tag1 transcripts and for high rates of somatic excision. this was true for the autonomous element in cauliflower mosaic virus 35s-tag1-beta-glucuronidase constructs and for a two-component system using the 35s promoter to produce tag1 transposase and a ... | 2001 | 11487697 |
| the rop gtpase switch controls multiple developmental processes in arabidopsis. | g proteins are universal molecular switches in eukaryotic signal transduction. the arabidopsis genome sequence reveals no ras small gtpase and only one or a few heterotrimeric g proteins, two predominant classes of signaling g proteins found in animals. in contrast, arabidopsis possesses a unique family of 11 rop gtpases that belong to the rho family of small gtpases. previous studies indicate that rop controls actin-dependent pollen tube growth and h(2)o(2)-dependent defense responses. in this ... | 2001 | 11402196 |
| constitutive overexpression of cytosolic glutamine synthetase (gs1) gene in transgenic alfalfa demonstrates that gs1 may be regulated at the level of rna stability and protein turnover. | glutamine synthetase (gs) catalyzes the atp-dependent condensation of nh4+ with glutanate to yield glutamine. gene constructs consisting of the cauliflower mosaic virus (camv) 35s promoter driving a cytosolic isoform of gs (gs1) gene have been introduced into alfalfa (medicago sativa). although transcripts for the transgene were shown to accumulate to high levels in the leaves, they were undetectable in the nodules. however, significant amounts of beta-glucuronidase activity could be detected in ... | 2001 | 11351075 |
| t-dna insertional mutagenesis for activation tagging in rice. | we have developed a new t-dna vector, pga2715, which can be used for promoter trapping and activation tagging of rice (oryza sativa) genes. the binary vector contains the promoterless beta-glucuronidase (gus) reporter gene next to the right border. in addition, the multimerized transcriptional enhancers from the cauliflower mosaic virus 35s promoter are located next to the left border. a total of 13,450 t-dna insertional lines have been generated using pga2715. histochemical gus assays have reve ... | 2002 | 12481047 |
| tomato plants ectopically expressing arabidopsis cbf1 show enhanced resistance to water deficit stress. | a dna cassette containing an arabidopsis c repeat/dehydration-responsive element binding factor 1 (cbf1) cdna and a nos terminator, driven by a cauliflower mosaic virus 35s promoter, was transformed into the tomato (lycopersicon esculentum) genome. these transgenic tomato plants were more resistant to water deficit stress than the wild-type plants. the transgenic plants exhibited growth retardation by showing dwarf phenotype, and the fruit and seed numbers and fresh weight of the transgenic toma ... | 2002 | 12376629 |
| down-regulation of tomato beta-galactosidase 4 results in decreased fruit softening. | transcript abundance of the gene encoding beta-galactosidase ii, a beta-galactosidase/exo-galactanase (ec 3.2.1.23) present during tomato (lycopersicon esculentum) fruit ripening, was suppressed by expression of an antisense tomato beta-galactosidase 4 (tbg4) cdna driven by the cauliflower mosaic virus 35s promoter. rna gel-blot analysis was used to evaluate tbg4 mrna levels in transgenic fruit. all of the antisense lines had attenuated tbg4 mrna levels in turning stage fruit; however, tbg4 mrna ... | 2002 | 12177488 |
| heterology expression of the arabidopsis c-repeat/dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato. | in an attempt to improve stress tolerance of tomato (lycopersicon esculentum) plants, an expression vector containing an arabidopsis c-repeat/dehydration responsive element binding factor 1 (cbf1) cdna driven by a cauliflower mosaic virus 35s promoter was transferred into tomato plants. transgenic expression of cbf1 was proved by northern- and western-blot analyses. the degree of chilling tolerance of transgenic t(1) and t(2) plants was found to be significantly greater than that of wild-type to ... | 2002 | 12114563 |
| delayed abscission and shorter internodes correlate with a reduction in the ethylene receptor leetr1 transcript in transgenic tomato. | stable transformation of tomato (lycopersicon esculentum cv ailsa craig) plants with a construct containing the antisense sequence for the receiver domain and 3'-untranslated portion of the tomato ethylene receptor (leetr1) under the control of an enhanced cauliflower mosaic virus 35s promoter resulted in some expected and unexpected phenotypes. in addition to reduced leetr1 transcript levels, the two most consistently observed phenotypes in the transgenic lines were delayed abscission and reduc ... | 2002 | 11891253 |
| constitutive overexpression of cystathionine gamma-synthase in arabidopsis leads to accumulation of soluble methionine and s-methylmethionine. | the committing step in met and s-adenosyl-l-met (sam) synthesis is catalyzed by cystathionine gamma-synthase (cgs). transgenic arabidopsis plants overexpressing cgs under control of the cauliflower mosaic virus 35s promoter show increased soluble met and its metabolite s-methyl-met, but only at specific stages of development. the highest level of met and s-methyl-met was observed in seedling tissues and in flowers, siliques, and roots of mature plants where they accumulate 8- to 20-fold above wi ... | 2002 | 11788756 |
| altered life cycle in arabidopsis plants expressing psugt1, a udp-glucuronosyltransferase-encoding gene from pea. | alfalfa (medicago sativa) and arabidopsis were used as model systems to examine molecular mechanisms underlying developmental effects of a microsomal udp-glucuronosyltransferase-encoding gene from pea (pisum sativum; psugt1). alfalfa expressing psugt1 antisense mrna under the control of the cauliflower mosaic virus (camv) 35s promoter exhibited delayed root emergence, reduced root growth, and increased lateral root development. the timing of root emergence in wild-type and antisense plants was c ... | 2003 | 12972656 |
| negative regulation of abscisic acid signaling by the fagus sylvatica fspp2c1 plays a role in seed dormancy regulation and promotion of seed germination. | fspp2c1 was previously isolated from beech (fagus sylvatica) seeds as a functional protein phosphatase type-2c (pp2c) with all the conserved features of these enzymes and high homology to abi1, abi2, and pp2ca, pp2cs identified as negative regulators of aba signaling. the expression of fspp2c1 was induced upon abscisic acid (aba) treatment and was also up-regulated during early weeks of stratification. furthermore, this gene was specifically expressed in aba-treated seeds and was hardly detectab ... | 2003 | 12970481 |
| long-distance root-to-shoot transport of phytochelatins and cadmium in arabidopsis. | phytochelatin synthases (pcs) mediate cellular heavy-metal resistance in plants, fungi, and worms. however, phytochelatins (pcs) are generally considered to function as intracellular heavy-metal detoxification mechanisms, and whether long-distance transport of pcs occurs during heavy-metal detoxification remains unknown. here, wheat tapcs1 cdna expression was either targeted to arabidopsis roots with the arabidopsis alcohol dehydrogenase (adh) promoter (adh::tapcs1/cad1-3) or ectopically express ... | 2003 | 12909714 |
| overexpression of the asn1 gene enhances nitrogen status in seeds of arabidopsis. | in wild-type arabidopsis, levels of asn1 mrna and asparagine (asn) are tightly regulated by environmental factors and metabolites. because asn serves as an important nitrogen storage and transport compound used to allocate nitrogen resources between source and sink organs, we tested whether overexpression of the major expressed gene for asn synthetase, asn1, would lead to changes in nitrogen status in the ultimate storage organ for metabolites-seeds. transgenic arabidopsis constitutively overexp ... | 2003 | 12805621 |
| expression of antisense acyl carrier protein-4 reduces lipid content in arabidopsis leaf tissue. | arabidopsis plants were transformed with acyl carrier protein (acp)-4 in antisense conformation driven by the cauliflower mosaic virus 35s promoter. it was hypothesized that reduction of acp4 in leaf tissue would result in a reduction in lipid biosynthesis and, in addition, affect fatty acid composition and leaf physiology. several transgenic lines have been generated with reduced acp4 protein in leaf tissue. dramatic reductions in acp4 resulted in a reduction of leaf lipid content (22%-60%) bas ... | 2003 | 12805604 |
| wvd2 and wdl1 modulate helical organ growth and anisotropic cell expansion in arabidopsis. | wild-type arabidopsis roots develop a wavy pattern of growth on tilted agar surfaces. for many arabidopsis ecotypes, roots also grow askew on such surfaces, typically slanting to the right of the gravity vector. we identified a mutant, wvd2-1, that displays suppressed root waving and leftward root slanting under these conditions. these phenotypes arise from transcriptional activation of the novel wave-dampened2 (wvd2) gene by the cauliflower mosaic virus 35s promoter in mutant plants. seedlings ... | 2003 | 12586874 |
| a lily asr protein involves abscisic acid signaling and confers drought and salt resistance in arabidopsis. | lla23, an abscisic acid-, stress-, and ripening-induced protein, was previously isolated from lily (lilium longiflorum) pollen. the expression of lla23 is induced under the application of abscisic acid (aba), nacl, or dehydration. to provide evidence on the biological role of lla23 proteins against drought, we used an overexpression approach in arabidopsis (arabidopsis thaliana). constitutive overexpression of lla23 under the cauliflower mosaic virus 35s promoter confers reduced sensitivity to a ... | 2005 | 16169963 |
| antisense suppression of deoxyhypusine synthase in tomato delays fruit softening and alters growth and development. | the effects of suppressing deoxyhypusine synthase (dhs) have been examined in tomato (solanum lycopersicum cv uct5). dhs mediates the first of two sequential enzymatic reactions that activate eukaryotic translation initiation factor-5a (eif-5a) by converting a conserved lys to the unusual amino acid, deoxyhypusine. dhs protein levels were suppressed in transgenic plants by expressing the 3'-untranslated region of tomato dhs under regulation of the constitutive cauliflower mosaic virus promoter. ... | 2005 | 15951486 |
| starch-related alpha-glucan/water dikinase is involved in the cold-induced development of freezing tolerance in arabidopsis. | cold-induced soluble sugar accumulation enhances the degree of freezing tolerance in various cold-hardy plants including arabidopsis (arabidopsis thaliana), where soluble sugars accumulate in only a few hours at 2 degrees c. hence, along with photosynthesis, starch degradation might play a significant role in cold-induced sugar accumulation and enhanced freezing tolerance. starch-related alpha-glucan/water dikinase (ec 2.7.9.4), encoded by arabidopsis starch excess 1 (sex1), is hypothesized to r ... | 2005 | 15894744 |
| plantacyanin plays a role in reproduction in arabidopsis. | plantacyanins belong to the phytocyanin family of blue copper proteins. in the arabidopsis (arabidopsis thaliana) genome, only one gene encodes plantacyanin. the t-dna-tagged mutant is a knockdown mutant that shows no visible phenotype. we used both promoter-beta-glucuronidase transgenic plants and immunolocalization to show that arabidopsis plantacyanin is expressed most highly in the inflorescence and, specifically, in the transmitting tract of the pistil. protein levels show a steep gradient ... | 2005 | 15908590 |
| immunopurification of polyribosomal complexes of arabidopsis for global analysis of gene expression. | immunoaffinity purification of polyribosomes (polysomes) from crude leaf extracts of arabidopsis (arabidopsis thaliana) was achieved with transgenic genotypes that overexpress a translational fusion of a ribosomal protein (rp) with a his(6)-flag dual epitope tag. in plants with a cauliflower mosaic virus 35s:hf-rpl18 transgene immunopurification with anti-flag agarose beads yielded 60-svedberg ribosomal subunits, intact 80-svedberg monosomes and polysomes. sucrose density gradient fractionation ... | 2005 | 15955926 |
| overexpression of a protein phosphatase 2c from beech seeds in arabidopsis shows phenotypes related to abscisic acid responses and gibberellin biosynthesis. | a functional abscisic acid (aba)-induced protein phosphatase type 2c (pp2c) was previously isolated from beech (fagus sylvatica) seeds (fspp2c2). because transgenic work is not possible in beech, in this study we overexpressed this gene in arabidopsis (arabidopsis thaliana) to provide genetic evidence on fspp2c2 function in seed dormancy and other plant responses. in contrast with other pp2cs described so far, constitutive expression of fspp2c2 in arabidopsis, under the cauliflower mosaic virus ... | 2006 | 16815952 |
| floral patterning defects induced by arabidopsis apetala2 and microrna172 expression in nicotiana benthamiana. | floral patterning and morphogenesis are controlled by many transcription factors including floral homeotic proteins, by which floral organ identity is determined. recent studies have uncovered widespread regulation of transcription factors by micrornas (mirnas), approximately 21-nucleotide non-coding rnas that regulate protein-coding rnas through transcript cleavage and/or translational inhibition. the regulation of the floral homeotic gene apetala2 (ap2) by mir172 is crucial for normal arabidop ... | 2006 | 16897492 |
| cinnamate metabolism in ripening fruit. characterization of a udp-glucose:cinnamate glucosyltransferase from strawberry. | strawberry (fragaria x ananassa) fruit accumulate (hydroxy)cinnamoyl glucose (glc) esters, which may serve as the biogenetic precursors of diverse secondary metabolites, such as the flavor constituents methyl cinnamate and ethyl cinnamate. here, we report on the isolation of a cdna encoding a udp-glc:cinnamate glucosyltransferase (fragaria x ananassa glucosyltransferase 2 [fagt2]) from ripe strawberry cv elsanta that catalyzes the formation of 1-o-acyl-glc esters of cinnamic acid, benzoic acid, ... | 2006 | 16443693 |
| the photorespiratory arabidopsis shm1 mutant is deficient in shm1. | mitochondrial serine hydroxymethyltransferase (shmt), combined with glycine decarboxylase, catalyzes an essential sequence of the photorespiratory c2 cycle, namely, the conversion of two molecules of glycine into one molecule each of co2, nh4+, and serine. the arabidopsis (arabidopsis thaliana) mutant shm (now designated shm1-1) is defective in mitochondrial shmt activity and displays a lethal photorespiratory phenotype when grown at ambient co2, but is virtually unaffected at elevated co2. the ... | 2006 | 16339799 |
| alteration of organic acid metabolism in arabidopsis overexpressing the maize c4 nadp-malic enzyme causes accelerated senescence during extended darkness. | the full-length cdna encoding the maize (zea mays) c(4) nadp-malic enzyme was expressed in arabidopsis (arabidopsis thaliana) under the control of the cauliflower mosaic virus 35s promoter. homozygous transgenic plants (mem) were isolated with activities ranging from 6- to 33-fold of those found in the wild type. the transformants did not show any differences in morphology and development when grown in long days; however, dark-induced senescence progressed more rapidly in mem plants compared to ... | 2007 | 17885087 |
| subcellular localization and functional domain studies of defective kernel1 in maize and arabidopsis suggest a model for aleurone cell fate specification involving crinkly4 and supernumerary aleurone layer1. | defective kernel1 (dek1), which consists of a membrane-spanning region (dek1-mem) and a calpain-like cys proteinase region (dek1-calp), is essential for aleurone cell formation at the surface of maize (zea mays) endosperm. immunolocalization and fm4-64 dye incubation experiments showed that dek1 and crinkly4 (cr4), a receptor kinase implicated in aleurone cell fate specification, colocalized to plasma membrane and endosomes. supernumerary aleurone layer1 (sal1), a negative regulator of aleurone ... | 2007 | 17933905 |
| posttranscriptional regulation of high-affinity sulfate transporters in arabidopsis by sulfur nutrition. | high-affinity sulfate transporters sultr1;1 and sultr1;2 are expressed at epidermis and cortex of arabidopsis (arabidopsis thaliana) roots during sulfur limitation. here, we report that sultr1;1 and sultr1;2 are two essential components of the sulfate uptake system in roots and are regulated at posttranscriptional levels together with the previously reported transcriptional control. double knockout of sultr1;1 and sultr1;2 by t-dna insertion gene disruption resulted in complete lack of sulfate u ... | 2007 | 17720755 |
| tagging of mads domain proteins for chromatin immunoprecipitation. | most transcription factors fulfill their role in complexes and regulate their target genes upon binding to dna motifs located in upstream regions or introns. to date, knowledge about transcription factor target genes and their corresponding transcription factor binding sites are still very limited. two related methods that allow in vivo identification of transcription factor binding sites are chromatin immunoprecipitation (chip) and chromatin affinity purification (chap). for chap, the protein o ... | 2007 | 17868439 |
| the gigantea-regulated microrna172 mediates photoperiodic flowering independent of constans in arabidopsis. | regulated rna metabolism appears to be a critical component of molecular mechanisms directing flowering initiation in plants. a group of rna binding proteins exerts their roles through the autonomous flowering pathway. posttranscriptional mechanisms regulated by micrornas (mirnas) also play a key role in flowering-time control. here, we demonstrate that the gigantea (gi)-regulated mir172 defines a unique genetic pathway that regulates photoperiodic flowering by inducing flowering locus t (ft) in ... | 2007 | 17890372 |
| a putative hydroxysteroid dehydrogenase involved in regulating plant growth and development. | we have functionally characterized an arabidopsis (arabidopsis thaliana) gene athsd1 (at5g50600) that encodes a protein with homology to animal 11-beta-hydroxysteroid dehydrogenase (hsd). transgenic arabidopsis plants overexpressing athsd1 (designated aohsd plants) under the control of the cauliflower mosaic virus 35s promoter showed increased growth and seed yield as well as increased tolerance of saline stress and reduced seed dormancy. in canola (brassica napus), transgenic plants overexpress ... | 2007 | 17616511 |
| roles of arabidopsis cyclin-dependent kinase c complexes in cauliflower mosaic virus infection, plant growth, and development. | the c-terminal domain (ctd) of rna polymerase ii is phosphorylated during the transcription cycle by three cyclin-dependent kinases (cdks): cdk7, cdk8, and cdk9. cdk9 and its interacting cyclin t partners belong to the positive transcription elongation factor b (p-tefb) complexes, which phosphorylate the ctd to promote transcription elongation. we report that arabidopsis thaliana cdk9-like proteins, cdkc;1 and cdkc;2, and their interacting cyclin t partners, cyct1;4 and cyct1;5, play important r ... | 2007 | 17468259 |
| two negative cis-regulatory regions involved in fruit-specific promoter activity from watermelon (citrullus vulgaris s.). | a 1.8 kb 5'-flanking region of the large subunit of adp-glucose pyrophosphorylase, isolated from watermelon (citrullus vulgaris s.), has fruit-specific promoter activity in transgenic tomato plants. two negative regulatory regions, from -986 to -959 and from -472 to -424, were identified in this promoter region by fine deletion analyses. removal of both regions led to constitutive expression in epidermal cells. gain-of-function experiments showed that these two regions were sufficient to inhibit ... | 2008 | 19073962 |
| highly boron deficiency-tolerant plants generated by enhanced expression of nip5;1, a boric acid channel. | boron (b) is an essential element for plants, and b deficiency is a worldwide agricultural problem. in b-deficient areas, b is often supplied as fertilizer, but excess b can be toxic to both plants and animals. generation of b deficiency-tolerant plants could reduce b fertilizer use. improved fertility under b-limiting conditions in arabidopsis thaliana by overexpression of bor1, a b transporter, has been reported, but the root growth was not improved by the bor1 overexpression. in this study, w ... | 2008 | 19017629 |
| badh2, encoding betaine aldehyde dehydrogenase, inhibits the biosynthesis of 2-acetyl-1-pyrroline, a major component in rice fragrance. | in rice (oryza sativa), the presence of a dominant badh2 allele encoding betaine aldehyde dehydrogenase (badh2) inhibits the synthesis of 2-acetyl-1-pyrroline (2ap), a potent flavor component in rice fragrance. by contrast, its two recessive alleles, badh2-e2 and badh2-e7, induce 2ap formation. badh2 was found to be transcribed in all tissues tested except for roots, and the transcript was detected at higher abundance in young, healthy leaves than in other tissues. multiple badh2 transcript leng ... | 2008 | 18599581 |
| iron-induced turnover of the arabidopsis iron-regulated transporter1 metal transporter requires lysine residues. | iron is an essential micronutrient but is toxic if accumulated at high levels. thus, iron uptake and distribution in plants are controlled by precise regulatory mechanisms. iron-regulated transporter1 (irt1) is the major high affinity iron transporter responsible for iron uptake from the soil in arabidopsis (arabidopsis thaliana). previously, we showed that irt1 is subject to posttranscriptional regulation; when expressed from the constitutive cauliflower mosaic virus 35s promoter, irt1 protein ... | 2008 | 18305211 |
| a versatile transposon-based activation tag vector system for functional genomics in cereals and other monocot plants. | transposon insertional mutagenesis is an effective alternative to t-dna mutagenesis when transformation through tissue culture is inefficient as is the case for many crop species. when used as activation tags, transposons can be exploited to generate novel gain-of-function phenotypes without transformation and are of particular value in the study of polyploid plants where gene knockouts will not have phenotypes. we have developed an in cis-activation-tagging ac-ds transposon system in which a t- ... | 2008 | 17993541 |
| flexible tools for gene expression and silencing in tomato. | as a genetic platform, tomato (solanum lycopersicum) benefits from rich germplasm collections and ease of cultivation and transformation that enable the analysis of biological processes impossible to investigate in other model species. to facilitate the assembly of an open genetic toolbox designed to study solanaceae, we initiated a joint collection of publicly available gene manipulation tools. we focused on the characterization of promoters expressed at defined time windows during fruit develo ... | 2009 | 19812183 |
| shoot na+ exclusion and increased salinity tolerance engineered by cell type-specific alteration of na+ transport in arabidopsis. | soil salinity affects large areas of cultivated land, causing significant reductions in crop yield globally. the na+ toxicity of many crop plants is correlated with overaccumulation of na+ in the shoot. we have previously suggested that the engineering of na+ exclusion from the shoot could be achieved through an alteration of plasma membrane na+ transport processes in the root, if these alterations were cell type specific. here, it is shown that expression of the na+ transporter hkt1;1 in the ma ... | 2009 | 19584143 |
| the cauliflower mosaic virus protein p6 forms motile inclusions that traffic along actin microfilaments and stabilize microtubules. | the gene vi product (p6) of cauliflower mosaic virus (camv) is a multifunctional protein known to be a major component of cytoplasmic inclusion bodies formed during camv infection. although these inclusions are known to contain virions and are thought to be sites of translation from the camv 35s polycistronic rna intermediate, the precise role of these bodies in the camv infection cycle remains unclear. here, we examine the functionality and intracellular location of a fusion between p6 and gfp ... | 2009 | 19028879 |
| a reporter gene under the control of tms or aux promoters is differentially expressed in tobacco and barley protoplasts. | agrobacterium tumefaciens and some agrobacterium rhizogenes strains possess auxin biosynthesis genes (tms and aux genes respectively), responsible for a de novo auxin biosynthetic pathway in transformed plant cells. a comparison is presented of the potential expression of these genes in a monocotyledonous (barley) and a dicotyledonous plant (tobacco). the promoters of the genes were translationally fused to the β-glucuronidase reporter gene and analysed in transient expression experiments. the t ... | 1994 | 24193642 |
| rol genes alter hormonal requirements for protoplast growth and modify the expression of an auxin responsive promoter. | growth characteristics of tobacco protoplasts containing rola linked to its own promoter, or the rolb, or rolc genes of agrobacterium rhizogenes linked to the cauliflower mosaic virus 35s rna promoter were compared with those from untransformed plants. rola protoplasts require auxin and cytokinin for callus formation. protoplasts overexpressing rolb and c form callus in the absence of exogenously applied auxin and cytokinin, respectively. long term callus growth requires auxin, but the requireme ... | 1993 | 24201783 |
| promotion of flowering and morphological alterations in atropa belladonna transformed with a camv 35s-rolc chimeric gene of the ri plasmid. | kanamycin-resistant plants of belladonna (atropa belladonna) were obtained after agrobacterium mediated transformation. when a rolc gene, which is one of the loci located on ri plasmid of agrobacterium rhizogenes, was co-introduced with a kanamycin resistant (npt ii) gene under control of a cauliflower mosaic virus 35s promoter, the rolc gene was expressed strongly in leaves, flowers, stems and roots. the transformed plants exhibited dramatic promotion of flowering, reduced apical dominance, pal ... | 1992 | 24201720 |
| transgenic herbicide-resistant atropa belladonna using an ri binary vector and inheritance of the transgenic trait. | transgenic atropa belladonna conferred with a herbicide-resistant trait was obtained by transformation with an ri plasmid binary vector and plant regeneration from hairy roots. we made a chimeric construct, park5, containing the bar gene encoding phosphinothricin acetyltransferase flanked with the promoter for cauliflower mosaic virus 35s rna and the 3' end of the nos gene. leaf discs of a. belladonna were infected with agrobacterium rhizogenes harboring an ri plasmid, pri15834, and park5. trans ... | 1992 | 24203127 |
| cloning of the coat protein gene from beet necrotic yellow vein virus and its expression in sugar beet hairy roots. | expression of the beet necrotic yellow vein virus (bnyvv) coat protein (cp) gene in transgenic sugar beet hairy roots was accomplished as a step towards cp-mediated virus resistance. a cdna for the cp gene and its 5' terminal untranslated leader sequence was prepared from bnyvv rna, using two oligodeoxynucleotides to prime the synthesis of both strands. second-strand synthesis and amplification of the cdna were done by taq dna polymerase chain reactions. run-off transcripts of the cloned cdna se ... | 1991 | 24221440 |
| construction of agrobacterium tumefaciens-mediated tomato black ring virus infectious cdna clones. | tomato black ring virus (tbrv, genus nepovirus) infects a wide range of economically important plants such as tomato, potato, tobacco and cucumber. here, a successful construction of infectious full-length cdna clones of the tbrv genomic rnas (rna1 and rna2) is reported for the first time. the engineered constructs consisting of pcr-amplified dnas were cloned into binary vector pjl89 immediately downstream of a double cauliflower mosaic virus (camv) 35s promoter, and upstream of the hepatitis de ... | 2017 | 28109755 |
| recombination-based generation of the agroinfectious clones of peanut stunt virus. | full-length cdna clones of peanut stunt virus strain p (psv-p) were constructed and introduced into nicotiana benthamiana plants via agrobacterium tumefaciens. the cdna fragments corresponding to three psv genomic rnas and satellite rna were cloned into pgreen binary vector between cauliflower mosaic virus (camv) 35s promoter and nopaline synthase (nos) terminator employing seamless recombinational cloning system. the plasmids were delivered into a. tumefaciens, followed by infiltration of hosts ... | 2016 | 27659243 |
| development of cotton leaf curl virus resistant transgenic cotton using antisense ßc1 gene. | cotton leaf curl virus (clcuv) is a serious pathogen causing leaf curl disease and affecting the cotton production in major growing areas. the transgenic cotton (gossypium hirsutum cv. coker 310) plants were developed by using βc1 gene in antisense orientation gene driven by cauliflower mosaic virus-35s promoter and nos (nopaline synthase) terminator and mediated by agrobacterium tumefaciens transformation and somatic embryogenesis system. molecular confirmation of the transformants was carried ... | 2016 | 27081361 |
| spike-dip transformation of setaria viridis. | traditional method of agrobacterium-mediated transformation through the generation of tissue culture had limited success for setaria viridis, an emerging c4 monocot model. here we present an efficient in planta method for agrobacterium-mediated genetic transformation of s. viridis using spike dip. pre-anthesis developing spikes were dipped into a solution of agrobacterium tumefaciens strain agl1 harboring the β-glucuronidase (gus) reporter gene driven by the cauliflower mosaic virus 35s (camv35s ... | 2016 | 26932666 |