| regulation of poly(a) site selection in adenovirus. | we have investigated the mechanisms involved in the early-to-late rna-processing switch which regulates the mrna species generated from the adenovirus major late transcription unit (mltu). in particular, polyadenylation choice mechanisms were characterized by using a reconstructed adenovirus e1a gene as a site for insertion of mltu poly(a) regulation signals (l1 and l3). adenovirus constructs containing the variant poly(a) recognition elements were used to compare e1a poly(a) signal utilization ... | 1989 | 2562992 |
| transformation-sensitive and growth-related changes of protein synthesis in ref52 cells. a two-dimensional gel analysis of sv40-, adenovirus-, and kirsten murine sarcoma virus-transformed rat cells using the ref52 protein database. | two-dimensional gels of normal and virally transformed ref52 cells have been quantified and compared using the quest system for construction and analysis of protein databases. the ref52 protein map is based on more than 1600 high quality spots, and the relative amounts of these proteins are studied in 79 gels representing 12 major experiments. ref52 cells transformed by sv40, adenovirus, and kirsten murine sarcoma virus (kimsv) are compared to normal ref52 cells at several stages of growth from ... | 1989 | 2564397 |
| sequences downstream of aauaaa signals affect pre-mrna cleavage and polyadenylation in vitro both directly and indirectly. | to investigate the role of sequences lying downstream of the conserved aauaaa hexanucleotide in pre-mrna cleavage and polyadenylation, deletions or substitutions were constructed in polyadenylation signals from simian virus 40 and adenovirus, and their effects were assayed in both crude and fractionated hela cell nuclear extracts. as expected, these sequences influenced the efficiency of both cleavage and polyadenylation as well as the accuracy of the cleavage reaction. sequences near or upstrea ... | 1989 | 2566911 |
| transcription termination within the e1a gene of adenovirus induced by insertion of the mouse beta-major globin terminator element. | in induced erythroleukemia cells, transcription of the beta-globin gene terminates in a region 600-1500 nucleotides downstream of the poly(a) site. to determine whether this region of the mouse dna functions to terminate transcription when moved to another genomic site, portions of the putative termination region have been inserted into the e1a transcription unit of the adenovirus (type 5) chromosome. analysis of rna labeled either in isolated nuclei or in whole cells early after infection with ... | 1985 | 2580640 |
| use of host cell reactivation of cisplatin-treated adenovirus 5 in human cell lines to detect repair of drug-treated dna. | this study demonstrates that whilst some dna-repair deficiencies can be detected using host cell reactivation of cisplatin (cddp)-treated adenovirus (ad5), not all repair deficiencies affected replication of cddp-treated ad5 in human cells. a line of fibroblasts (xp25), derived from a patient with a uv-hypersensitive syndrome xeroderma pigmentosum (xp), was found, as previously reported [1], to be deficient in reactivating the treated virus when compared to the apparently repair-proficient human ... | 1989 | 2582540 |
| characterization of the simian adenovirus type 30 inverted terminal repeat. | the presence of an inverted terminal repeat (itr), which plays an important role in the initiation of dna replication, is one of the characteristic properties of adenoviruses (ads). we have established the nucleotide (nt) sequences for the itr of simian adenovirus type 30 (sv30), a subgroup-iii oncogenic virus. this repeat consists of 185 nt, representing the longest itr found in an ad so far. it contains multiple copies of internal repeats, as well as the consensus sequences of the putative bin ... | 1989 | 2583517 |
| anti-idiotype response to selected neutralizing monoclonal antibodies generated against adenovirus type 2 fiber. | a series of monoclonal antibodies was prepared against purified adenovirus type 2 fiber. these antibodies were produced by both classical techniques and by electrofusion. fifty antibodies were obtained, and five were selected which had the following characteristics: (i) they were able to reduce adenovirus 2 infection of hep-2 cells; (ii) they had different light-chain structures and (iii) they did not react with denatured fiber. the five antibodies (adf18, adf38, adf41, adf2d3, adf3b1) recognize ... | 1989 | 2621062 |
| biochemical analysis of adenovirus type 5 dna-binding protein mutants. | we previously reported the isolation and functional characterization of seven adenovirus type 5 (ad5) dna-binding protein (dbp) point mutants (quinn, c. o., and kitchingman, g. r. (1986) j. virol. 60, 653-661). six of the seven mutants were defective in their ability to help adeno-associated virus replicate its dna. to determine the level at which the mutations affect this function of the dbp, we analyzed several properties of the mutant proteins. all are transported to the nucleus and are post- ... | 1989 | 2644280 |
| [biological effects accompanying the microinjection of adenovirions, adenoviral and plasmid dna into mammalian cells]. | microinjection of either type 1 human adenovirus, type sa7 monkey adenovirus virions or circular adenovirus dna, obtained by the treatment of dna-terminal protein complexes with glutaraldehyde, into nuclei of permissive cells results in the complete cycle of virus reproduction. microinjection of neither linear native, condensed adenovirus dna nor the dna-terminal protein complexes under the same conditions initiates the adenovirus reproduction thought the synthesis of early and some late viral a ... | 1989 | 2685544 |
| [immunoenzyme analysis of adenovirus hexon. determination of antibodies from hyperimmunized chickens]. | we have elaborated three systems of enzyme-linked immunosorbent assay (elisa) for detection of chicken igg antibodies specific for hexon antigens of three immunologically distinct adenovirus groups: those of mammalian adenoviruses (mastadenovira), typical avian adenoviruses (aviadenovira) and of egg-drop syndrome-76 (eds-76) virus. in each system the antibodies against respective hexons were specifically detected. in mammalian adenovirus hexons the elisa detects primarily the type-specific (epsi ... | 1989 | 2693958 |
| [malpighian epithelia infected by dna viruses and langerhans cells]. | malpighian epithelia exhibit an immune surveillance through langerhans cells (lc) against the dna viruses, which induce various epithelial lesions, mainly on the skin, conjunctiva, mouth, uterine cervix. these viral infections are usual during childhood. the most common dna viruses are: adenoviruses (more than 40 types), human papillomaviruses (hpv, more than 50 types) and herpes viruses (herpes simplex type 1 and 2, varicella and zooster, epstein-barr virus). different clinical aspects are obse ... | 1989 | 2694082 |
| adenoviruses as vectors for the transfer of genetic information and for the construction of new type vaccines. | at present many types of corpuscular nondefective, conditional-defective and helper-dependent expressing adenoviral vectors are available which can be used in constructing gene-engineered live or inactivated viral vaccines. in particular, promising results have been obtained with live recombinant human adenoviruses expressing the s antigen of hepatitis b virus, capsid protein of rotaviruses and gb protein of herpes virus. these recombinants are proper candidates for testing as corresponding vacc ... | 1989 | 2694817 |
| the oncogenicity of avian adenoviruses. iv. confirmatory evidence for recombination between viral and cellular dna sequences and repetition of the recombinant in cells of a tumor line. | on the basis of hybridization analyses with viral dna fragments as probes, we previously concluded that, in dnas of many fowl adenovirus type 1-induced rodent tumors, units consisting of viral dna segments flanked on both sides by cellular dna segments are repeated more than 100 times per diploid genome of cell. molecular cloning of virus-cellular junction fragments and analyses of their sequence arrangement in the present study confirmed that the previous conclusion was basically correct. | 1989 | 2705306 |
| formation of large, sedimentable transcription complexes with varna genes and other related genes. | we have used an eppendorf centrifuge for isolation of transcription complexes assembled on varna genes and other related genes with ntp-depleted cell-free extracts. similar to the 5 s rrna gene, sedimentable, stable transcription preinitiation complexes could be assembled from two varna genes, two eb virus-specific eber genes, four human trna genes, and one human alu-family rna gene, suggesting that the 5 s rrna-specific transcription factor, tfiiia, was not required for formation of these sedim ... | 1989 | 2722876 |
| a heteroploid permanent cell line originating from embryonic calf thyroid supporting the replication of all known bovine adenovirus serotypes. | an embryonic calf thyroid cell culture was established as a permanent heteroploid cell line, which is now in its 150th subculture. it allowed replication of all nine bovine adenovirus serotypes at its 15th as well as its 60-150th passages. all viruses induced typical cytopathic effects. yields obtained on the permanent calf thyroid line were, on average, 0.8 log10 lower than those obtained on primary calf testicle cells. | 1989 | 2750074 |
| adenovirus l1 52- and 55-kilodalton proteins are required for assembly of virions. | a variant of adenovirus type 5 that contained a mutation within the l1 52- and 55-kilodalton (52/55k) protein-coding region was isolated. the mutant, termed ts369, produced l1 52/55k proteins with a two-amino-acid substitution and was temperature sensitive. temperature-shift experiments indicated that the ts369 defect was late in the viral growth cycle. dna replication and synthesis of late proteins occurred normally in ts369-infected cells at the nonpermissive temperature, but mature virions we ... | 1989 | 2760976 |
| interactions of minute virus of mice and adenovirus with host nucleoli. | biochemical evidence is presented that both minute virus of mice (mvm) and adenovirus interact with the nucleolus during lytic growth and that mvm can also target specific changes involving nucleolar components in adenovirus-infected cells. these virus-nucleolus interactions were studied by analysis of intranuclear compartmentalization of both viral dnas and host nucleolar proteins: (i) mvm in mouse cells (its normal host) replicates its dna in the host nucleoli; (ii) specific nucleolar proteins ... | 1989 | 2760977 |
| only a minor fraction of plasma membrane-associated large t antigen in simian virus 40-transformed mouse tumor cells (mksa) is exposed on the cell surface. | the bulk of simian virus 40 (sv40) large t antigen in sv40-infected and -transformed cells localizes within the cell nucleus, while a minor fraction specifically associates with the plasma membrane (pm) and is exposed on the cell surface. pm-associated large t seems to span the lipid bilayer but, on the other hand, does not display typical features of a transmembrane protein. to further characterize the postulated transmembrane orientation of large t, we asked whether all large t molecules assoc ... | 1989 | 2760985 |
| oral vaccination of dogs fed canine adenovirus in baits. | six groups of 5 dogs each were fed dilutions of canine adenovirus-2, either as raw liquid or after insertion into cornmeal baits. by the fourth week after vaccination, 29 of the 30 dogs developed high titers of serum-neutralizing antibodies to the virus. | 1989 | 2764336 |
| simian adenovirus type 7 (sa-7) induces tumours of nerve-supporting or paraneural cell origin in newborn hamsters. | simian adenovirus type 7 (sa-7) was found to induce tumours originating from nerve-supporting or paraneural cells in newborn hamsters, regardless of injection site or tissues. sa-7 induces glioblastomas characterized by definite localization (subependymal regions) and its main cell type, bipolar spongioblast-like cells, in the brain of hamsters inoculated as newborns. when the eyes of newborn hamsters were directly inoculated, sa-7 failed to induce retinoblastoma (0/27), but retro or peri-bulbar ... | 1989 | 2765394 |
| tissue-specific dna binding of nuclear proteins that bind to the adenovirus inverted terminal repeat. | adenovirus (ad) dna replication requires nuclear factor i (nfi), a cellular sequence-specific dna binding protein which binds to the ad dna inverted terminal repeat (itr). the nfi binding consensus sequence, tggn6-7gccaa, possibly includes the ccaat-box which is often observed in regulatory elements of transcription. adjacent to the nfi binding site on the ad itr is the binding site for nuclear factor iii (nfiii), another cellular factor that stimulates ad dna replication in a cell-free system. ... | 1989 | 2768219 |
| mouse anti-adenovirus cytotoxic t lymphocytes. inhibition of lysis by e3 gp19k but not e3 14.7k. | early region e3 of adenovirus (ad) appears to encode proteins involved in the interaction of the virus with the host immune system. the e3 region 19-kda glycoprotein (gp19k) binds to class i mhc ag in the endoplasmic reticulum and inhibits their transport to the cell surface; it has been proposed that this protects virus infected cells from lysis by ctl. we have found that the e3 14.7-kda protein (14.7k) inhibits lysis of infected cells by tnf, and here we show that it also protects cells from l ... | 1989 | 2789254 |
| monoclonal antibody technique in detecting adenovirus antigens of virus pneumonia in children. | | 1987 | 2820661 |
| mutational analysis of the adenovirus e1a gene: the role of transcriptional regulation in transformation. | to determine whether the transcription regulatory activities of the adenoviral e1a gene play a role in its ability to transform primary cells we have constructed an extensive series of mutations within the e1a gene. the mutants have been characterized for their ability to transactivate the adenoviral early promoters, repress the transcriptional stimulation of the polyoma virus enhancer, establish primary baby rat kidney cells in culture and cooperate with the activated ha-ras oncogene in morphol ... | 1987 | 2820718 |
| value and cost effectiveness of double culture tests for diagnosis of ocular viral and chlamydial infections. | swabbings from the eyes of 4132 patients attending ophthalmic casualty and outpatients clinics were tested for chlamydiae, adenovirus, and herpes simplex virus. laboratory isolation tests gave positive results for one of these three agents in 696 (16.8%) cases. when a positive isolation was obtained, only 341 (49%) agreed with the clinical diagnosis while 355 (51%) either had no definite diagnosis marked on the request card or had been clinically diagnosed incorrectly. routine testing of ocular ... | 1987 | 2822080 |
| evidence for the role of a human intestinal adenovirus in the pathogenesis of coeliac disease. | we previously noted a region of amino acid sequence homology between a-gliadin, a major alpha-gliadin component known to activate coeliac disease, and the early region e1b protein of human adenovirus serotype 12 (ad12), an adenovirus isolated from the human intestinal tract. in the present study sera from coeliac disease patients from the united kingdom and the united states were assayed for neutralising antibody to ad12 as evidence of past exposure to that virus and for antibody to synthetic pe ... | 1987 | 2822550 |
| adenovirus infection associated with respiratory disease in commercial chickens. | the lesions and etiologic agents associated with 13 outbreaks of respiratory disease in commercial chickens were investigated. adenoviruses were isolated from tracheal and lung tissues of affected chickens in all 13 outbreaks. escherichia coli was isolated from the lung of an occasional bird. the tracheal specimens were consistently negative for bordetella avium, but e. coli and occasionally staphylococcus aureus were isolated. there was also serological evidence in one outbreak, and pathologica ... | 1987 | 2823779 |
| cryogenic preservation of virus-infected cells used as immunofluorescent assay substrates. | cells infected with herpes simplex virus, respiratory syncytial virus and adenovirus were subjected to several cryogenic regimens to determine whether these procedures were detrimental to the performance and results obtained with an indirect immunofluorescence assay (ifa). infected cells were harvested and divided into equal aliquots prior to freezing at different temperatures (-20, -70 and -165 degrees c). cells fixed onto glass slides immediately after harvesting were used as controls. for the ... | 1987 | 2824546 |
| a small deletion distant from a splice or polyadenylation site dramatically alters pre-mrna processing in region e3 of adenovirus. | the e3 complex transcription unit of adenovirus encodes overlapping mrnas (a to i) with different exon structures. the major mrnas are a (approximately 40% of the total) and c (approximately 15%), which are spliced once, and f (approximately 15%) and h (approximately 25%), which are spliced twice. mrna a uses the upstream e3a polyadenylation site, and the other mrnas use the downstream e3b polyadenylation site. we analyzed virus deletion mutants to identify sequences important in alternative pre ... | 1987 | 2824824 |
| regulation of thyroidal inducibility of na,k-atpase and binding of epidermal growth factor in wild-type and cold-sensitive e1a mutant type 5 adenovirus-transformed cref cells. | we have analyzed the relationship between expression of the transformed phenotype and thyroid hormone (triiodothyronine, t3) inducibility of na,k-atpase and binding of 125i-epidermal growth factor (egf) to cell membrane receptors in wild-type (wt) and mutant type 5 adenovirus (ad5)-transformed cref cells displaying a cold-sensitive (cs) expression of the transformed phenotype. cref cells respond to thyroid hormone treatment with increased na,k-atpase activity and bind similar levels of 125i-egf ... | 1987 | 2826499 |
| latex agglutination test for adenovirus diagnosis in diarrheal disease. | a commercial latex agglutination test for diagnosis of adenovirus in diarrheal disease (adenolex, orion diagnostica, finland) was evaluated by comparison with the results obtained by elisa, electron microscopy (em), and virus isolation. fifty specimens originated from the diagnostic routine, and 50 were selected from a previous epidemiological study on the etiology of diarrheal disease in children. thirteen of the 100 specimens reacted with the latex control, impairing interpretation of the resu ... | 1987 | 2826673 |
| adenovirus pneumonia. | adenoviruses are ubiquitous dna viruses that cause a wide variety of illnesses, including pneumonia, in children and adults. forty-one distinct human sero-types have been identified, yet only about six of these serotypes are associated with the majority of the cases of adenovirus-induced pneumonia. adeno-virus infection accounts for up to 20% of childhood pneumonias, primarily in those children younger than 5 years of age, but such pneumonias occur infrequently in the nonmilitary adult populatio ... | 1987 | 2827279 |
| ctl recognition of adenovirus-transformed cells infected with influenza virus: lysis by anti-influenza ctl parallels adenovirus-12-induced suppression of class i mhc molecules. | we examined the ability of influenza-specific cytotoxic t lymphocytes to lyse adenovirus-transformed cells infected with influenza virus. cytotoxic t lymphocyte lysis of ad12-transformed cells was greatly reduced relative to that of ad5-transformed cells. lysability of adenovirus-12-transformed cells was restored in parallel with interferon-gamma induced increases in major histocompatibility complex class i gene products. these findings establish that recognition of foreign molecules by self-res ... | 1988 | 2827381 |
| two new genome types of adenovirus 7c. | adenovirus type 7 is the type most frequently associated with serious disease. eighteen different genome types of adenovirus type 7 had been reported up to october 1986. the genome type ad7c, based on the restriction enzyme profiles of smai and bamhi, has been reported from europe prior to 1969 and more recently from south africa. here, we report two new genome types of adenovirus 7 c that have not previously been identified and that have been isolated in south africa between 1975 and 1986 from ... | 1988 | 2828528 |
| roentgenographic features of common pediatric viral respiratory tract infections. | viral infections of the respiratory tract in infants and children are common. little has been reported on roentgenographic findings associated with infection caused by common viruses other than with respiratory syncytial virus. we studied chest roentgenograms from 128 previously healthy infants and children who were infected with respiratory syncytial virus, parainfluenza virus, influenza virus, or adenovirus. four common roentgenographic findings were detected: parahilar peribronchial infiltrat ... | 1988 | 2829619 |
| a 64 kd nuclear protein binds to rna segments that include the aauaaa polyadenylation motif. | a 64 kd protein was shown to bind to rnas that contain functional polyadenylation signals by a uv cross-linking procedure in which label was transferred from rna substrate to protein in cell-free polyadenylation extracts. the 64 kd nuclear protein bound specifically to three different substrates (adenovirus type 5 l3, sv40 early, and sv40 late polyadenylation domains), as determined by competition experiments and partial protease analysis. deleted derivatives of the sv40 late substrate that reta ... | 1988 | 2830023 |
| complementation for replication by unrelated animal viruses containing dna genomes. | | 1987 | 2830477 |
| adult follicular conjunctivitis and neonatal ophthalmia in a liverpool eye hospital, 1980-1984. | in the five year period between 1980 and 1984, 2146 adults and 172 neonates suffering from acute conjunctivitis underwent laboratory investigation for chlamydia trachomatis (ct), adenoviruses (av), herpes simplex virus (hsv) and pathogenic bacteria. epidemiology and clinical features are presented and discussed. ct was detected in 29 per cent of neonates with conjunctivitis. 5.6 per cent of adults and older children investigated for follicular conjunctivitis were ct positive. there was a signifi ... | 1987 | 2832220 |
| [immune response of calves following vaccination with paramyxovirus parainfluenzae-3 and adenovirus-1 under different colostral immunity stresses]. | studied was the immune response of calves, with colostrum antibodies and without colostrum antibodies, immunised with different dosages of live bivalent vaccine against p. parainfluenzae-3 and adenovirus-1. for the group of calves with colostrum immunity, after single hypodermic immunisation with doses from 10(4) till 10(5) cpe50/cm3 for adenovirus, was not proved immune response, as a result of blocking of the vaccinal antigen of colostrum antibodies. for calves which missed these antibodies, t ... | 1987 | 2833005 |
| expression of the hsv-2 ribonucleotide reductase subunits in adenovirus vectors or stably transformed cells: restoration of enzymatic activity by reassociation of enzyme subunits in the absence of other hsv proteins. | we have cloned the large subunit (rr1) of the hsv-2 ribonucleotide reductase into a helper-independent adenovirus 5 vector under control of the viral major late promoter. infection of 293 cells with the adred-1 recombinant virus resulted in the expression of the hsv-2 rr1 protein. we have also produced cells which constitutively express the small (rr2) subunit of the hsv-2 enzyme by transfecting 293 cells with a plasmid encoding this protein and the neo resistance marker (psv2neo-rr2). infection ... | 1988 | 2833019 |
| bovine adenovirus type-3 infection in feedlot calves. | the frequency of bovine adenovirus type-3 (ba3) infection in 84 of 165 light feeder calves and the possible role of ba3 in inducing febrile disease and affecting weight gain performance were studied for 56 days after the calves entered a feedlot. samples of blood for serotesting were obtained periodically, and samples for virus isolation were collected on days 28 and 56. at the time the calves entered the feedlot, 17.8% (15 of 84) had serum virus-neutralizing antibodies to ba3. during 56 days, 5 ... | 1988 | 2833138 |
| vaccination of dairy calves with bovine adenovirus type 3. | a field study was undertaken to determine the efficacy of vaccinating dairy calves with a killed bovine adenovirus type 3 (ba3) vaccine in a herd where pneumonia associated with ba3 infection had been a severe problem. calves were first vaccinated when they were less than one week old; a second dose was given 10-14 days later. efficacy of the vaccine was evaluated following natural exposure to the virus by comparing prevalence of pneumonia in control (n = 21) and vaccinated (n = 21) calves. sero ... | 1987 | 2833376 |
| efficient transcription, not translation, is dependent on adenovirus tripartite leader sequences at late times of infection. | to determine whether the tripartite leader is required for efficient translation in adenovirus-infected cells at late times of infection, we constructed recombinant adenoviruses containing the influenza virus nucleocapsid protein (np) gene expressed under the control of the adenovirus major late promoter (mlp). we chose the np gene because previous results showed that the influenza virus np mrna was an extremely effective initiator of translation in cells which were superinfected with influenza ... | 1988 | 2833610 |
| the adeno-associated virus rep gene inhibits replication of an adeno-associated virus/simian virus 40 hybrid genome in cos-7 cells. | a hybrid adeno-associated virus (aav)/simian virus 40 (sv40) genome is described. in this construct sv40 regulatory sequences, including the early promoter/enhancers and origin of dna replication, were substituted for the aav p5 promoter, which normally controls expression of the aav rep gene. the hybrid genome was phenotypically indistinguishable from wild-type aav in human cells in the presence or absence of helper virus. upon transfection into cos-7 cells, which constitutively produced the sv ... | 1988 | 2833621 |
| transcriptional but not translational regulation of hiv-1 by the tat gene product. | human immunodeficiency virus-1 (hiv-1), which causes aids (acquired immune deficiency syndrome), possesses an essential gene, tat, whose product, acting through the long terminal repeat (ltr) sequences of hiv-1, activates viral genes and replication. the mechanism by which tat trans-activates hiv genes is unclear. some studies have reported that an increase in messenger rna accumulation directed by the hiv-1 ltr can explain the action of tat, but others suggest that this increase in mrna levels ... | 1988 | 2833703 |
| adenovirus type-2 in a patient with lethal hemorrhagic colonic ulcers and chronic active epstein-barr virus infection. | an adenovirus type-2 was isolated from peripheral blood lymphocytes and throat washings from a patient with severe chronic active epstein-barr virus infection. despite the epstein-barr virus reactivation, attempts to establish spontaneous lymphoblastoid cell lines from peripheral blood lymphocytes and to immortalize cord lymphocytes with throat washings were unsuccessful due to a marked cytopathic effect. the supernatants from the cultures induced cytopathic effect in cultured cord lymphocytes, ... | 1988 | 2833864 |
| abundant expression of herpes simplex virus glycoprotein gb using an adenovirus vector. | herpes simplex virus type 1 (hsv-1) glycoprotein b (gb) is a major component of infected cell membranes and virion envelopes. glycoprotein b is known to be essential for entry of viruses into cells and may play important roles in virus-induced cell fusion and other alterations in cell morphology. in order to study the biochemical and immunological properties of gb in isolation from other hsv-1 polypeptides we have constructed human adenovirus vectors capable of expressing high levels of gb. the ... | 1988 | 2834864 |
| constraints on spacing between transcription factor binding sites in a simple adenovirus promoter. | the adenovirus 2 e1b transcription unit has an extremely simple promoter consisting of a tata box and a closely situated gc box. the interaction between the tata box and the gc box was analyzed using insertion mutations which expand the distance between the two elements. we observed that the e1b promoter has an unusually rigid architecture. when the gc box, which is a binding site for transcription factor sp1, was separated further from the tata box than in the wild-type promoter, in vivo transc ... | 1988 | 2836264 |
| a new epstein-barr virus transactivator, r, induces expression of a cytoplasmic early antigen. | several epstein-barr virus (ebv) early promoters respond to a new ebv transactivator encoded by brlf1, designated r. transactivation was measured in chloramphenicol acetyltransferase assays on raji, bhk, and vero cells that were cotransfected with the transactivator and target promoters linked to the cat gene. the divergent promoter of bamhi-h was particularly responsive to r transactivation. this large promoter region consists of a leftward tata box for the noti repeat gene (bhlf1) and a probab ... | 1988 | 2836611 |
| prevalence of antibodies to seven viruses in a flock of ewes in minnesota. | blood samples were collected from a flock of healthy ewes at a university of minnesota research station. sera from these blood samples were tested for antibodies against 7 viruses, using 3 tests (eg, virus-neutralization test for bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, bovine adenovirus type 3, and bovine respiratory syncytial virus; hemagglutination inhibition test for parainfluenza virus type 3; and agar-gel immunodiffusion test for lentivirus of ovine progressive ... | 1988 | 2837112 |
| pathogenicity studies with a strain of fowl adenovirus serotype 8 (vri-33) in chickens. | experiments were undertaken to study the pathogenesis of vri-33, a strain of fowl adenovirus serotype 8 isolated from the liver of a broiler chicken with inclusion body hepatitis. a 30% death rate resulted from oral infection of one-day-old specific pathogen free chickens with 10(6) plaque forming units of vri-33. chickens 10, 14, 21 and 28 days of age did not die following infection via natural routes but there were some motalities following infection via parenteral routes. immunodepression by ... | 1987 | 2838009 |
| initiation of adenovirus dna replication. i. mechanism of action of a host protein required for replication of adenovirus dna templates devoid of the terminal protein. | the in vitro replication of adenovirus (ad) dna covalently attached to the 55-kda terminal protein requires at least five proteins including the 80-kda preterminal protein, the ad dna polymerase, the ad dna binding protein, nuclear factor i, and topoisomerase i. the replication of ad dna templates devoid of the terminal protein requires an additional protein, designated factor pl, which has been purified from uninfected hela cell nuclei (guggenheimer, r. a., nagata, k., kenny, m., and hurwitz, j ... | 1988 | 2838479 |
| initiation of adenovirus dna replication. ii. structural requirements using synthetic oligonucleotide adenovirus templates. | adenovirus (ad) virions contain a 55-kda terminal protein covalently linked to both 5'-ends of the linear duplex dna genome. the origin of dna replication is contained within the terminal 50 base pair of the inverted terminal repeats. in the accompanying paper (kenny, m. k., balogh, l. a., and hurwitz, j. (1988) j. biol. chem. 263, 9801-9808), it was demonstrated that synthetic oligonucleotide templates which contain the ad origin, but lack the 55-kda terminal protein, can serve as templates for ... | 1988 | 2838480 |
| [inflammatory sequelae after adenovirus infection]. | adenovirus infections are common. the period of virus replication is short, hardly ever exceeding two weeks. in 216 patients we studied the post-inflammatory period after infection with adenovirus types 8, 4, 3, 7, 19 and 37, in some patients up to three years. in 49.5%, the initial infection also involved the cornea, in 79.5% bilaterally. in 50.5% there was only follicular conjunctivitis. the post-inflammatory period is most annoying as rather frequently a considerable period of chronic eye irr ... | 1988 | 2838542 |
| synthesis of p34, the mammalian homolog of the yeast cdc2+/cdc28 protein kinase, is stimulated during adenovirus-induced proliferation of primary baby rat kidney cells. | the homolog of the cdc2+/cdc28+ encoded protein kinases has previously been identified in hela cells by immunological methods. here we have studied the distribution and synthesis of this 34 kd protein in rat tissues and cell lines. p34 was found in a variety of organs, including some such as brain that are not highly active in cell division. kidney has very low levels of p34. however, proliferative activation of baby rat kidney cells with adenovirus caused rapid induction of p34 synthesis. induc ... | 1988 | 2838787 |
| infection by multiple viruses and lymphocyte abnormalities at the diagnosis of diabetes. | evidence for an ongoing adenovirus infection and preceding eb and coxsackie b virus infections was found in a 2-year-old boy at the diagnosis of insulin-dependent diabetes. an inverted t-helper/t-suppressor lymphocyte ratio and activation of t cells with a suppressor-cytotoxic phenotype were detected. a combination of multiple viral infections may be responsible for the rapid destruction of pancreatic beta cells in this case. | 1988 | 2839003 |
| viral gene inhibition of class i major histocompatibility antigen expression: not a general mechanism governing the tumorigenicity of adenovirus type 2-, adenovirus type 12-, and simian virus 40-transformed syrian hamster cells. | the association between the level of class i major histocompatibility (mhc) antigen expression and the tumorigenic phenotype was determined for cells from a series of 15 lines of adenovirus type 2 (ad2)-, ad12-, and simian virus 40 (sv40)-transformed hamster cells and 16 lines of cells established from hamster tumors induced by sv40 mutants. these cells range from nontumorigenic to highly tumorigenic in both syngeneic and allogeneic adult hamsters. the ad2-transformed cells--cells that were nont ... | 1988 | 2839700 |
| immune electron microscopy in the detection of viruses other than enteroviruses on cell culture in untreated sewage. | following the demonstration of enteroviruses in samples of untreated sewage by inoculation of cell cultures with an eluate fraction, immune electron microscopy (iem) was employed in two stages to detect adenoviruses and the hepatitis a virus (hav). 6/12 samples contained adenoviruses and 5/15 other samples contained viruses having characteristics of hav. cell culture can thus be usefully allied with iem in the more precise determination of the potential health hazards of waste water. | 1988 | 2839942 |
| characterization of canine adenovirus type 1 isolated from american black bears. | viruses recovered from tissues taken at necropsy from american black bears were examined by use of immunofluorescence with polyclonal and monoclonal antibodies, virus neutralization with monoclonal antibodies, and restriction endonuclease analyses of the viral genomes. with these techniques, viruses were determined to be canine adenovirus type 1. seronegative dogs that were inoculated with the virus had clinical signs typical of infectious canine hepatitis, suggesting that the virus, which was v ... | 1988 | 2840839 |
| a new hamster model for adenoviral vaccination. | both adult and baby hamsters infected intranasally with human adenovirus type 5 exhibited virologic, serologic, and histologic evidence of infection. when 8-day old hamsters were infected with 4 x 10(6) pfu, concentrations of virus up to 2 x 10(6) pfu/animal were detected in the lung, peaking on day 2. the minimum infectious dose was 1 x 10(3) pfu/animal. this model may be useful in studies of conventional and recombinant adenoviral vaccines for humans. | 1988 | 2840876 |
| chicken embryo propagation of type i avian adenoviruses. | forty-two clone-purified, cell-culture-propagated type i avian adenoviruses (aav) representing 11 serotypes and two intermediate strains were evaluated for virus replication (evidenced by embryo death and lesions) resulting from the inoculation of specific-pathogen-free chicken embryos via the chorioallantoic sac or yolk sac. commonly observed embryonic changes were death, stunting and curling, hepatitis, splenomegaly, congestion and hemorrhage of body parts, and urate formation in the kidneys. ... | 1988 | 2840884 |
| gastrointestinal viral infections in homosexual men who were symptomatic and seropositive for human immunodeficiency virus. | gastrointestinal viruses, predominantly rotaviruses and adenoviruses, were detected by enzyme-linked immunosorbent assay, electron microscopy, or cell culture in greater than 50% of two groups of homosexual men with symptomatic human immunodeficiency virus (hiv) infection, who did (54%) or did not (50%) have diarrhea. lower detection rates were observed in hiv-seronegative (15%) and asymptomatic hiv-seropositive (16%) men. in the patients with diarrhea, 95% of the isolates of virus were found in ... | 1988 | 2841379 |
| nosocomial adenovirus infections: molecular epidemiology of an outbreak due to adenovirus 3a. | an immunocompromised woman died of disseminated infection due to adenovirus type 3. during her hospitalization and after her death, 38 hospital personnel developed an acute respiratory illness. adenovirus type 3 infection was documented by culture in 18 of the 38 individuals and by seroconversion in one additional employee. four of 34 asymptomatic hospital personnel exposed to the index case also seroconverted. thus, 23 personnel were considered to have confirmed infections, and 22 of these 23 r ... | 1988 | 2841380 |
| associations between viral infection and respiratory disease in young beef bulls. | blood samples were taken from bull calves at two meat and livestock commission performance testing centres, just after weaning at six months of age and at six weekly intervals until the end of the performance test seven months later. sera were assayed by specific elisas for antibodies to bovine herpes virus 1 (bhv1), respiratory syncytial virus, parainfluenza 3 (pi3) and adenoviruses a and b. seroconversions between each sampling were related to the occurrence of clinical respiratory disease usi ... | 1988 | 2842921 |
| expression and rescue of a nonselected marker from an integrated aav vector. | we used rep+ and rep- recombinant aav-plasmid vectors containing the nonselectable marker chloramphenicol acetyltransferase (cat) driven by the aav p40 promoter, and having a selectable marker, neo, inserted in the plasmid genome, and driven by a herpesvirus thymidine kinase gene promoter. each vector was transfected into human 293 cells or hela cells and the neo gene was used to select geneticin-resistant (genr) cells containing integrated vectors. the genr cells were then screened for expressi ... | 1988 | 2842941 |
| initiation of viral dna replication. | | 1988 | 2843015 |
| [2 outbreaks of adenovirus 8 keratoconjunctivitis. clinical, epidemiological and preventive approach]. | adenovirus 8 represents the main agent of epidemic keratoconjunctivitis (ekc) and outbreaks mainly occur in hospital wards, colleges and metallurgy industry. adenovirus 8 conjunctivitis are generally severe lasting two to three weeks, and keratitis with persistent infiltrates may be observed for more than a year. there is no actual treatment of ekc and therefore preventive measures appear as essential to avoid and/or limit such outbreaks in hospital wards. we have observed two consecutive outbre ... | 1988 | 2843589 |
| viral infections in acute peripheral facial paralysis. nationwide analysis centering on cf. | the degree of participation and regional specificity of virus infection in relation to atraumatic acute peripheral facial palsy was studied, placing particular emphasis on change in the cf titre of varicella zoster virus (vzv), herpes simplex virus (hsv) and adenovirus (adeno). the subjects of the study were 91 patients with hunt's syndrome and 396 patients with bell's palsy treated at 17 institutions all over japan in the period between april 1985 and november 1986. among the cases of hunt's sy ... | 1988 | 2844057 |
| [gastrointestinal findings in hiv infection. clinical aspects, microbiological findings and endoscopic picture]. | among 200 hospitalized patients treated for hiv infections there were 98 with gastrointestinal symptoms, independent of the stage of the disease. only 22 had abnormal stool findings. but histological examination and culture of endoscopically obtained biopsies revealed opportunistic infection in 62, of whom 28 had a cytomegalovirus infection. mycobacterium avium-intracellulare was found in the gastrointestinal mucosa of 25 patients, but its clinical significance is unclear. in 33 of the 98 patien ... | 1988 | 2844496 |
| expression of the epstein-barr virus encoded ebna-1 gene in stably transfected human and murine cell lines. | five murine and 3 human tumor cell lines were transfected with a retroviral vector that carries the ebv encoded ebna-1 gene. all cell lines expressed intranuclear ebna-1 as detected by anticomplement immunofluorescence and western blot assays. the cell lines differed in the level of ebna-1 expression and the size of the protein. the internal major late promoter of adenovirus was efficient in directing the transcription of ebna-1 in the human lymphoma line bjab, the murine t-cell lymphoma tikaut, ... | 1988 | 2844682 |
| influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3y1 fibroblasts and their derivatives transformed by a wide variety of agents. | we compared proliferation and survival of various syngeneic transformed cell lines under conditions of depletion of 15 amino acids in dulbecco-eagle's medium. we used a normal fibroblast line 3y1 and 22 transformed sublines of 3y1 which had been induced by one of seven transforming agents--simian virus 40, mouse polyomavirus, adenovirus type 12, e1a gene of adenovirus type 12, cdna of harvey murine sarcoma virus, rous sarcoma virus, or n-methyl-n'-nitro-n-nitrosoguanidine. unlike other untransfo ... | 1988 | 2844831 |
| an epidemic of adenovirus 7a infection in a neonatal nursery: course, morbidity, and management. | an epidemic of adenovirus 7a in our neonatal intensive care nursery and intermediate care nursery in july and august 1987 caused the death of two patients. significant symptomatic infection possibly due to the virus occurred in nine patients, ten staff, and three parents, of whom three patients, three staff, and one parent were positive by culture. as a direct consequence of the outbreak, 58 staff days of work were lost; the intensive care nursery had to be closed to admissions for 19 days and t ... | 1988 | 2844886 |
| [morphologic studies of virus infection of the intestinal tract--virus replication and cytopathology in cell cultures and enterocytes. 3. replication of porcine adenoviruses in cell culture compared to that of rota- and coronaviruses]. | | 1988 | 2847681 |
| an adenovirus infection of the kidney of franklin's ground squirrels (spermophilus franklini) in saskatchewan, canada. | during routine pathological studies of franklin's ground squirrels (spermophilus franklini) collected during a predator control program, basophilic intranuclear inclusions were found in the collecting tubule epithelium of the renal papillae in seven of 13 squirrels. this was associated with marked karyomegaly in affected cells. an inflammatory response was not seen in the adjacent tissues. electron microscopic examination of affected cells demonstrated that the enlarged nuclei contained numerous ... | 1988 | 2848139 |
| the presence of both antivirus and antiself antibodies in sera from patients with adenovirus and influenza b. | using the elisa method we examined serum samples from 62 male patients aged 19-23 infected with adenovirus (serotype 7), 22 children aged 7-14 infected with influenza b (b/norway 1/84) and 113 normal subjects aged 5-30. the infections were diagnosed serologically by complement fixation, by inhibition of hemagglutination, by elisa and by viral culture. moreover using enzyme-linked short-time culture assay, the production of specific antivirus antibodies and autoantibodies in vitro by spleen cells ... | 1988 | 2848359 |
| adenovirus infection in families. | eighteen families were followed up for four to six weeks after one member of each family was diagnosed as having an adenovirus infection. in 17 of 18 index cases the diagnosis was based on the rapid detection of adenovirus hexon antigen in the nasopharyngeal mucus specimens and in one case (the only adult index case) on isolation of the virus. all index cases had high temperatures associated most commonly with tonsillitis, acute otitis media, gastroenteritis, or febrile convulsions. in 14 of the ... | 1988 | 2848459 |
| some observations on the response of ring-necked pheasants to inoculation with various strains of cell-culture-propagated type ii avian adenovirus. | the response of ring-necked pheasants to inoculation with three strains of cell-culture-propagated type ii avian adenovirus was examined. marble spleen disease (msd) virus of pheasants and both avirulent and virulent strains of hemorrhagic enteritis virus (hev) of turkeys all induced typical gross and microscopic splenic lesions of msd; neither msd-associated lung lesions nor mortality were noted in inoculated pheasants, regardless of strain of virus used. pheasants inoculated with a cell-cultur ... | 1988 | 2848487 |
| membrane d2 t antigen: characterization and comparison with its nuclear counterpart. | up to 40% of membrane t antigen (d2 t antigen) produced by the adenovirus 2--simian virus 40 hybrid, ad2+d2, remained tightly associated with membranes under alkaline conditions up to ph 11.5. the antigen could not be totally solubilized by treatment with ionic detergents, nonionic detergents, or both. these properties are characteristic of integral membrane proteins. sephacryl s-300 chromatography in high salt in the presence of brij-99 showed that the nuclear form of d2 t antigen was dissociat ... | 1988 | 2848548 |
| cooperation of v-oncogenes in human epithelial cell transformation. | the development of tissue culture systems for propagation of human epithelial cells has aided the investigation of events that lead epithelial cells to become neoplastic. in the present study, nontumorigenic human epidermal keratinocytes, immortalized by ad12-sv40 virus or psv3-neo, were transformed by a variety of retroviruses containing bas, h-ras, fes, fms, erbb and src oncogenes. such transformants showed morphological alterations and induced carcinomas when transplanted into nude mice. thes ... | 1988 | 2848992 |
| charge configurations in viral proteins. | the spatial distribution of the charged residues of a protein is of interest with respect to potential electrostatic interactions. we have examined the proteins of a large number of representative eukaryotic and prokaryotic viruses for the occurrence of significant clusters, runs, and periodic patterns of charge. clusters and runs of positive charge are prominent in many capsid and core proteins, whereas surface (glyco)proteins frequently contain a negative charge cluster. significant charge con ... | 1988 | 2849101 |
| contact lens disinfection to prevent transmission of viral disease. | current fda standards for contact lens disinfecting systems require that viricidal activity be demonstrated against just one strain of herpes simplex virus, type 1. small and nonenveloped viruses (e.g., adenoviruses) may be more resistant to disinfection than herpes simplex virus (hsv); however, the efficacy of disinfection systems against these other agents is not routinely tested. currently approved methods of chemical and thermal lens disinfection do appear to be efficient means to inactivate ... | 1988 | 2850120 |
| camp acts in synergy with e1a protein to activate transcription of the adenovirus early genes e4 and e1a. | the transcriptional control regions of several e1a-inducible adenovirus early genes contain sequences similar to the camp response element of several cellular camp-inducible genes. the camp-responsive cell line s49 was infected with wild-type adenovirus and found to contain elevated levels of mrnas encoded by all early genes tested (e4, e1a, and e1b), following treatment with dibutyryl camp. this effect was at the level of transcriptional activation. the effect of camp on e4 and e1a transcriptio ... | 1988 | 2850966 |
| [etiology of pneumonia in children]. | | 1988 | 2851127 |
| [carrier state of enteric viruses in ivory coast pregnant women in good health]. | the present work emphasize the great diversity of the viral species encountered in pregnant women (rotavirus, coronavirus, adenovirus, poliovirus, coxsackievirus). in each of the different regions studied, one or two viral types are usually predominant. in man (west ivory coast) region for instance, rotaviruses and coronaviruses were mainly encountered; in bouaké, polioviruses and adenoviruses were predominant whereas in bondoukou and odienné respectively coxsackieviruses and adenoviruses were p ... | 1988 | 2851394 |
| [avian celo adenovirus as a possible contaminant in cell cultures of japanese quail embryos]. | avian adenovirus celo was found to replicate poorly in japanese quail embryos (jqe) and cell cultures of them. the infectious process in these systems was latent. the antigen of adenovirus celo in jqe cell culture was detectable by the fluorescent antibody method (fam) within the first 24-72 hours after inoculation as fluorescent cytoplasmic granules. subsequently, fluorescence of nuclei and macrophage cytoplasm was observed. the results indicate that jqe and their cell cultures are not contamin ... | 1988 | 2854677 |
| antiviral activities of 2'-deoxyribofuranosyl and arabinofuranosyl analogs of sangivamycin against retro- and dna viruses. | eight sugar-modified pyrrolopyrimidine nucleoside analogs related to the antibiotic sangivamycin were evaluated in cell culture against herpes simplex types 1 (hsv-1) and 2 (hsv-2), cytomegalovirus (cmv), adenovirus, and visna virus. five of the compounds were highly active against most of the viruses with 50% inhibition (ed50) values of 1-10 microm. the selectivity of the agents was low, with inhibition of uninfected cell proliferation occurring within 5-fold that of the virus ed50 for most of ... | 1988 | 2854957 |
| [antiviral activity of new derivatives of quaternary phosphonium]. | | 1986 | 2855848 |
| [viral risk and ophthalmologic instrumentation]. | | 1988 | 2856082 |
| the role of macrophages in adenovirus-induced immunosuppression in mice. | a decreased humoral immune response to sheep red blood cells (srbc) was demonstrated in cflp mice inoculated with human adenovirus type 6 (ad6 virus). the suppressive effect depended on the inoculation sites of the virus and srbc. inoculation by the same route, i.e. intraperitoneally (i.p.), resulted in a significant decrease of the humoral immune response to srbc. when administering the virus and srbc at different sites, no inhibitory effect could be detected. immunosuppression was prevented by ... | 1985 | 2859763 |
| immunomodulation by bordetella pertussis: antiviral effects. | treatment of mice by intraperitoneal inoculation of pertussis vaccine or lipopolysaccharide extracted from b. pertussis will effect resistance to rabies virus, encephalomyocarditis virus, semliki forest virus, and herpes simplex virus. our previous observations indicated that treatment of c3h/hen (+/nu) and bdf1 mice with pertussis vaccine injected i.p. five days prior to a mouse adenovirus lethal dose i.p. challenge elicited resistance to clinical disease and death. susceptibility returned to a ... | 1985 | 2872109 |
| involvement of prostaglandins in immunosuppression caused by adenovirus in mice. | the effect of intraperitoneally (i.p.) inoculated human adenovirus type 6 (ad6) was tested for humoral immune response against sheep red blood cells (srbc) in normal and indomethacin-treated mice, with the aim to elucidate the mode of the virus action in immunosuppression. the results indicate that inhibition of prostaglandin synthesis slightly influences the immunosuppressive effect of the virus. it is very likely that also other mechanisms are involved in the immunosuppression observed. | 1987 | 2883861 |
| replication of adenovirus and sv40 chromosomes in vitro. | as an approach to studying the mechanisms involved in the replication of eukaryotic chromosomes, we have developed and characterized cell-free replication systems for the animal viruses, adenovirus and sv40. in this report we summarize recent work on the proteins required for the initiation of dna synthesis in these two systems. the adenovirus origin of dna replication was shown to consist of three functionally distinct sequence domains. cellular proteins that specifically recognize each of thes ... | 1987 | 2894680 |
| the role of cytoskeleton and nuclear matrix in virus replication. | in the light of the cytoarchitecture concept, the presented review attempts to cover what is known of the involvement of cytoskeletal and nucleoskeletal elements in the replication cycle of various viruses and in cell transformation. our knowledge on the relationship of virus replication with cell architecture has rapidly progressed during the recent years in association with studies on composition of various filaments within cells, their spatial organization and possible functions which focusse ... | 1988 | 2899966 |
| effects of adriamycin and etoposide on the replication of adenovirus 5 in sensitive and resistant human tumour cells. | adenovirus is a potential probe for identifying and understanding drug sensitivity in primary, nonproliferating cultures of human normal and tumour cells but the scope and limitations of such an approach first need to be evaluated in established cell lines. for this purpose we have identified an ovarian tumour cell line (ci-80-13s) with natural resistance to adriamycin, etoposide and crosslinking agents compared with other human tumour lines. resistance to adriamycin correlated poorly with resis ... | 1989 | 2910307 |
| possible role of adp-ribosylation of adenovirus core proteins in virus infection. | we have investigated the role of poly(adp)-ribosylation of adenoviral proteins in virus infection. viral core proteins v and the precursor to protein vii were shown to be in vivo and in vitro acceptors of adp-ribose. in vivo adp-ribosylation was restricted to viral proteins as the histones were not labeled during the late phase of infection. the adp-ribosylated core proteins were assembled into mature virus particles. in vitro adp-ribosylation of adenoviral core proteins performed with purified ... | 1986 | 2941933 |
| hepatoma variants (c2) are defective for transcriptional and post-transcriptional actions from both endogenous and viral genomes. | a series of rat hepatoma cell lines was infected with a recombinant adenovirus bearing the rat albumin promoter. transcription from this promoter was scored directly and was highest in fao, the differentiated parent, undetectable in c2, a cell variant that has lost almost all hepatocytic characteristics, and high again in c2-rev7, a 'revertant' cell line derived from c2 that has regained the ability to produce many proteins characteristic of hepatocytes. the endogenous albumin gene is not transc ... | 1987 | 2956094 |
| activation of adenovirus promoters by the adenovirus e1a protein in cell-free extracts. | the primary product of the adenovirus e1a gene is a protein that is sufficient for controlling host-cell proliferation and immortalizing primary rodent cells. the mechanism by which the protein induces these cellular effects is poorly understood, but might be linked to its ability to regulate rna transcription from a number of viral and cellular genes. the mechanism of e1a's transcriptional-activation (trans-activation) was studied here by monitoring the protein's effect on specific adenovirus p ... | 1987 | 2956686 |
| activation of an insect baculovirus promoter in mammalian cells by adenovirus functions. | the insect baculovirus autographa californica nuclear polyhedrosis virus (acnpv) replicates in insect cell lines in culture. in mammalian cells, however, the virus cannot be propagated. acnpv dna does not replicate or persist and is not transcribed in mammalian cells (tjia et al., 1983). in insect cells productively infected with acnpv, at least two major late viral gene products have been recognized, the polyhedrin, which makes up the bulk of the polyhedral inclusion bodies in infected cell nuc ... | 1987 | 2963453 |
| in vivo evolution of adenovirus 2-transformed cell virulence associated with altered e1a gene function. | neoplastic cell populations may evolve to a state of higher virulence in immunocompetent hosts. transforming gene involvement in this process of tumor progression was evaluated using adenovirus type 2 (ad2)-transformed hamster cells that are highly susceptible to destruction by natural killer cells and activated macrophages, due to ad e1a gene function, and are nontumorigenic in immunocompetent animals. cells selected for increased tumorigenicity retained parental cell patterns of viral gene int ... | 1988 | 2965448 |
| characterization of adenovirus type 40 e1 region. | the left-most 3.9 kb of adenovirus type 40 (ad40) dna has been sequenced using cloned viral dna fragments. the ad40 e1 region is deduced to code for at least four polypeptides, 221 and 249 amino acids as e1a products in addition to 166 and 475 amino acids as e1b products. e1b polypeptides share about 50% homology with well-defined adenovirus types, 2/5, 7, and 12, throughout the e1b sequences. e1a homology of ad40 to these types is relatively lower than that of e1b, while highly conserved region ... | 1988 | 2968714 |