| properties of bispecific rosette-forming cells. ii. --rosette formation by "educated" t-lymphocytes. | normal thymus cells transferred into thymectomized and lethally irradiated syngeneic mice responded to stimulation with sheep erythrocytes by rosette formation with these erythrocytes. this response reached a peak on the 7th day and was not associated with any production of circulating antibodies. rosettes produced by "educated" t-cells were inhibited by anti-theta serum as well as by anti-mouse fab serum. simultaneous stimulation of transferred thymocytes with sheep and pigeon erythrocytes prov ... | 1975 | 52333 |
| two igg1 (igf) subclasses in mice: presence in normal serum and representation within lymphocyte and plasma cell populations. | monospecific anti-gamma1 sera were prepared in rabbits against each of two igg1 mouse myeloma globulins isolated from myeloma a2 and mopc21. both these sera react with igg1 of normal mouse serum by immunoelectrophoresis and cannot be distinguished any further. the antibodies contained in the sera were cross-isolated on immunoadsorbents containing either the a2 or mopc21 antigenic material and tested by immunoelectrophoresis against normal mouse serum. an interesting spur was formed between these ... | 1975 | 52334 |
| [sensitivity tests of malignant tumours against cytostatic agents in vitro and in vivo/studies on the mouse sarcoma 180 (author's transl)]. | the effects of ten different substances on the mouse sarcoma 180 have been compared using in vivo and in vitro test systems. the size of the tumours was taken as a measure of the success of the therapy in animal experiments. the in vitro effects were estimated by measuring the incorporation of radioactively labelled uridine in tumour cell suspensions from solid tumours, in ascites tumours and in tissue culture. similar results were obtained using all three in vitro test systems. four substances ... | 1975 | 52364 |
| sister chromatid exchange and chromosome organization based on a bromodeoxyuridine giemsa-c-banding technique (tc-banding). | hoechst 33258 fluorescent staining of bromodeoxyuridine substituted chromosomes provided a high resolution technique for following the segregation of replicated chromosomal dna (latt, 1973). modifications have produced the same results after giemsa staining (wolff and perry, 1975). since this does not necessarily require hoechst (korenberg and freedlander, 1975), we call this bromodeoxyuridine-giemsa banding (bg-banding). we here describe a further modification which allows one to follow the t-r ... | 1975 | 52432 |
| a mouse hepatoma cell line which secretes several serum proteins including albumin and alpha-foetoprotein. | a permanent cell line (bw) was established from a transplantable mouse hepatoma, bw7756, which produces alpha-foetoprotein (afp). three clones were isolated from the uncloned culture: bw1, bw2 and bwtg3. the cells of the latter clone, which was isolated after selection in the presence of thioguanine, are deficient in the enzyme hypoxanthine-guanine-phosphoribosyl transferase. both bw1 and bwtg3 cells have mean chromosome number of 64 (60 telocentric and 4 metacentric chromosomes). all three clon ... | 1975 | 52568 |
| tumor localization of radiolabeled antibodies raised by a mouse plasma cell tumor. | | 1975 | 52604 |
| specificity of the antibody response in inbred mice to bovine type i and type ii collagen. | mouse antibodies to soluble bovine skin (type i) collagen react with determinants which are located in the rigid triple-helical portion of the antigen and become destroyed upon unfolding the molecule. helical antigenic determinants are dependent on the genuine chain assembly, e.g. alpha[1(i)]2alpha2. artefactual triplehelical structures of the composition [alpha1(i)]3 or [alpha2]3 or a genetically distinct type ii collagen from cartilage showed no or only weak cross-reactivity. pepsin treatment ... | 1975 | 52615 |
| studies on the control of antibody synthesis. vii. change in affinity of direct and indirect plaque-forming cells with time after immunization in the mouse: loss of high affinity plaques late after immunization. | the change in avidity of anti-hapten antibody with time after immunization was studied in mice at the level of the antibody-forming cell. a progressive increase in avidity was seen in both direct and indirect plaque-forming cells. late (38 days) after immunization with a large dose of antigen there was a preferential loss of high avidity plaque-forming cells and the average avidity decreased. high avidity memory cells were still present since boosting resulted in the prompt appearance of very hi ... | 1975 | 52616 |
| the potentiality of antibody-producing cells. ii. evidence for two antibody molecules of different specificities secreted by micromanipulated bispecific mouse spleen cells. | bispecific pfc appearing on the 4th day after immunization of mice with sheep erythrocytes conjugated with trinitrophenyl hapten (tnp-srbc) were identified by their capacity to lyse native srbc and tnp-conjugated horse erythrocytes (tnp--horbc) simultaneously in an open carboxymethylcellulose medium. individual pfc thus detected, were micromanipulated into two successive media containing the indicators srbc and tnp--horbc. out of 103 transferred double cells ninety-two (89 per cent) remained dou ... | 1975 | 52617 |
| cell co-operation and hapten--carrier complexes. | the co-operation of spleen cells of carrier- and hapten--carrier-primed mice in antibody formation against the hapten part of complexes was studied in 550 rad whole body irradiated mice. hapten--carrier complexes were prepared with the 2,4-dinitrophenyl group (dnp) as a hapten and heterologous bovine serum albumin (bsa) and isologous mouse immunoglobulin (mig) as carriers. priming of donor mice with carrier alone did not prepare for a secondary (igg) response in the recipients of hapten--carrier ... | 1975 | 52618 |
| immune complexes in the spleen. replacement of immune complexes trapped in spleen follicles by new immune complexes from the circulation. | the fate of intravenously injected 125i-bgg-anti-bgg in the spleen of mice was studied using autoradiography. part of the labelled immune complexes was trapped in the follicles of the spleen as could be expected. in a first experiment it was found that injections with unlabelled immune complexes were followed by a partial release of the labelled immune complexes from the follicles. in a second experiment unlabelled immune complexes retained in spleen follicles appeared to inhibit the trapping of ... | 1975 | 52623 |
| antigenic variation among parainfluenza type 1 (sendai) viruses: analysis of 6/94 virus. | 6/94 virus, isolated originally from a multiple sclerosis (ms) patient, was compared antigenically with related parainfluenza type 1 strains. these included two sendai strains of mouse and two sendai strains of reported human origin as well as the ha2 strain. by standard hemagglutination inhibition (hi) or hemadsorption neutralization (had-n) tests or by the complement-fixation (cf) cross-block titration test for detecting surface antigens, 6/94 virus and the sendai virus strains were indistingu ... | 1975 | 52630 |
| an antigenically related tryptic polypeptide from several mammalian type c rna virus p30s. | limited tryptic digestion of p30 antigen (the major internal viral protein) of type c viruses isolated from hamster, rat, and gibbon ape yielded a polypeptide fragment with a molecular weight of approximately 10,000 daltons. some antigenic determinants with interspecies specificity were retained on these polypeptides, which in serological tests cross-reacted with antibody produced previously against a similar fragment obtained from mouse p30. exhaustive trypsinization resulted in further fragmen ... | 1975 | 52631 |
| immunogenic properties of modified antigen e. iii. effect of repeated injections of modified antigen on immunocompetent cells specific for native antigen. | it has been shown that ragweed antigen e loses its major antigenic determinants after denaturation in 8 m urea, but urea-denatured (ud) antigen and an alpha-polypeptide chain isolated from the denatured molecules are capable of priming mouse t cells specific for native antigen. weekly injections of 10mug ud antigen or alpha-chain into antigen e-primed animals depressed the ongoing ige antibody response, whereas injections of the same dose of antigen e failed to depress the antibody response. it ... | 1975 | 52668 |
| effect of hypocholesterolemic drug ay9944 on cultured nervous tissue: morphologic and biochemical studies. | the effects of different concentrations of the hypocholesterolemic drug ay9944, an inhibitor of delta7-reductase, on organotypic cultures of fetal mouse spinal cord, were studied by light and electron microscopy. exposure to 10(-6)m produced no observable changes. after 6 hours exposure to 10(-4)m, dense membrane-bound inclusions were occasionally observed in neurons. after 24 hours exposure to 10(-4)m, numerous cytoplasmic inclusions occurred in neurons, glia and macrophages. the form of these ... | 1975 | 52692 |
| isolation of an endogenous c-type rna virus from mus musculus molossinus. | we isolated a type-c rna virus from the japanese field mouse, mus musculus molossinus. m. musculus musculus and m. musculus molossinus are two different subspecies of mus and thus only distantly related. the virus grew only on cells foreign to the host, was xenotropic, and readily rescued the murine sarcoma (musv) genome from a normal rat kidney cell line transformed nonproductively by the harvey strain of musv. the virus banded at a density of 1.16 g/ml and contained an rna-dependent dna polyme ... | 1975 | 52716 |
| autogenous immunity to endogenous rna tumor virus: reactivity of natural immune sera to antigenic determinants of several biologically distinct murine leukemia viruses. | sera from normal (c57bl/6xc3h/anf)f1(b6c3f1) mice reacted with several biologically distinct murine leukemia virus(es) (mulv) by radioimmune precipitation assays with the use of purified tritiated leucine-labeled virus. the reactivities of this natural antibody to viral envelope antigens of two laboratory strains (rauscher and moloney) and two endogenous mouse c-type viruses (akr and balb:virus-2) were further analyzed and compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. si ... | 1975 | 52718 |
| tumor-associated antigens in isoantigenic variants of a 3-methylcholanthrene-induced sarcoma. | a sarcoma was induced in an (a.ca x a.by)f1 mouse. two isoantigenic variants were selected by loss of one h-2 antigen. the tumor-associated transplantation antigens (tata) of these variants were compared as to their specificities in (a.ca x a.by)f1 mice. both transplantation and indirect membrane immunfluorescence tests revealed that tata of both variants did not cross-react. thus selecting angainst different h-2 antigens also selected different tata. karyotype studies suggested that both varian ... | 1975 | 52721 |
| temperature-dependent rosette formation by mouse lymphoma cells as a result of viral hemadsorption. | cells from several mouse lymphomas formed rosettes with nonsensitized foreign erythrocytes through c-type virus particles clustered on the cell surface in serum-free medium held at 4 degrees c. this type of rosetting was found most typically in a lymphoma induced by rauscher leukemia virus in tissue culture (rd-12), but it also occurred in 23 of 61 spontaneous thymic lymphomas in akr mice. chemically or x-ray-induced leukemias and spontaneous reticulum cell sarcomas did not form rosettes. the na ... | 1975 | 52722 |
| trials in man with live recombinants made from a/pr/8/34 (h0 n1) and wild h3 n2 influenza viruses. | a long-term study is described of recombinant influenza viruses produced from the avirulent laboratory strain, a/pr/8/34 (h0 n1), and newly isolated h3 n2 influenza virus variants. a number of h3 h2 recombinants were found to be attenuated for man and capable of inducing antibody formation, and were therefore potentially usable as live vaccines. however, the volunteer trials as a whole suggested that, in this system, there might not be complete segregation of virulence and antigenic characterist ... | 1975 | 52768 |
| staining method for whole-body autoradiography. | sagittal whole-body sections of frozen mice were cut on a hydraulicly driven microtome in a cryostat at--15 c by applying cotton or nylon-backed adhesive tape to the mouse before cutting. section thickness was 20 mu. the sections, still adhering to the tape, were dried in the cryostat (-15c) under atmospheric pressure. after autoradiography, the sections were pressed to a glass slide spread with a mixture of albumin and glycerin. the slide was immersed in xylene at 30 c for 15 min. the tape was ... | 1975 | 52917 |
| embryology of the epidermis: ultrastructural aspects. ii. period of differentiation in the mouse with mammalian comparisons. | a detailed light and electron microscopic study of the cellular morphology of the epidermis in the 13 through 16 day mouse fetus reveals that an occasional intermediate cell is interposed between the basal and peridermal layers on day 13. all layers are mitotically active. tonofilaments, unassociated with desmosomes, are present within the basal cell cytoplasm and the mitotic axis of the basal cells has changed from a parallel to a perpendicular plane with respect to the epidermal surface. at 14 ... | 1975 | 52965 |
| visualization of nucleolar organizer regions im mammalian chromosomes using silver staining. | a simple ammoniacal silver staining procedure, designated ag-as, differentially stains the chromosomal locations of ribosomal dna in certain mammalian species. this was critically demonstrated by ag-as staining of the nucleolus organizer regions in karyotypes of the same species and cell lines used for locating the ribosomal cistrons by dna/rna in situ hybridization. with ag-as, silver stained nors (ag-nors) are visualized as black spherical bodies on yellow-brown chromosome arms. ag-nors were v ... | 1975 | 53131 |
| urinary metabolites from gamma- and beta-bhc in the mouse: chlorophenol conjugates. | metabolites produced from gamma- and beta-bhc in the mouse urine were purified and characterized. most metabolites from both isomers were not extractable by non-polar organic solvents and were conjugates such as sulfates and glucuronides. after hydrolysis with an appropriate enzyme, the conjugates gave chlorophenols, among which 2,4,6-trichlorophenol existed most abundantly (total, about 25% in the urine metabolites). 2,4-dichlorophenol also constituted a significant portion. | 1975 | 53148 |
| effect of the quality of the lipopolysaccharide on mouse virulence of salmonella enteritidis. | the cell wall o antigen is known to affect mouse virulence of salmonella. we have shown earlier that s. typhimurium expressing somatic antigen 9,12 is less virulent than its 4,12 sister transductants in mice after intraperitoneal inoculation, suggesting that the 4,12-type o antigen is connected with high virulence in mice. in this report we show, in accord with this suggestion, that when the naturally occurring o-9,12 s. enteritidis is made o-4,12 by transduction, its virulence is increased. the ... | 1975 | 53202 |
| antigen-binding cells in human fetal liver. | antigen-binding cells (abc) could be detected regularly by autoradiography among haemic cells in the liver of human fetuses ranging in age from 8 to 24 weeks. for radioiodine-labeled thyroglobulin, the antigen mainly used in these studies, counts of abc ranged from 5.0 to 24.3 per 1,000 cells scanned. there was a trend for counts of abc in liver to be highest at 10-12 weeks of fetal life. binding of labeled thyroglobulin was inhibited by excess unlabeled thyroglobulin, but not by other protein a ... | 1975 | 53207 |
| inhibition of sensitization of t-cells by alpha-fetoprotein. | a two-phase model of allograft immunity was studied. in the first phase, specific immune t-cells were generated by incubation of responder cells with mitomycin-c treated allogeneic stimulator cells of the same h-2 type as mouse mastocytoma tumor cells. in the second step, the ability of the sensitized cells to kill cr51-labelled p-815 mastocytoma cells was assayed. alpha-fetoprotein (afp) was shown to inhibit the generation of immune cytotoxic t-cells at low concentrations (1-100 ng/ml) when add ... | 1975 | 53209 |
| characterizaiton of two populations of human lymphocytes bearing easily detectable surface immunoglobulin. | two separate lymphocyte populations, each bearing easily detectable surface immunoglobulin, have been detected in human peripheral blood. the first, b cells, has surface determinants that are stable at 37degreec, but are removed by pronase and regenerate in culture. the cells are nylon adherent and have a receptor for c3, and studies with unit gravity sedimentation indicate they are mostly small lymphocytes. b cells comprise 9.5% of the total lymphocytes, with the normal range from 3-16%. as man ... | 1975 | 53240 |
| induction of antiphosphorylcholine antibody formation by anti-idiotypic antibodies. | anti-idiotypic antibodies have been used to mimic antigen in the mouse antiphosphorylcholine response in order to investigate the induction of precursors of antibody-forming cells. we have shown that interaction of anti-idiotype antibody with receptor antibody molecules induces the formation of antibodies that are specific for phosphorylcholine and carry the idiotypic determinants. this induction is dependent on the recognition of carrier determinants on the anti-idiotype antibody by helper t ce ... | 1975 | 53257 |
| the major histocompatibility complex determines susceptibility to cytotoxic t cells directed against minor histocompatibility antigens. | cytotoxic cells were generated by immunizing one strain of mouse with cells from an allogeneic strain which carries the same h-2 region. the effector cells assayed in a 4 h 51cr release assay were shown to be t cells and indistinguishable, except in specificity, from cytotoxic t cells directed at h-2 alloantigens. although the genetic differences between responder and stimulator cells responsible for the immunization did not code in h-2, the h-2 complex did restrict susceptibility of target cell ... | 1975 | 53263 |
| induction of cytotoxic t lymphocytes against i-region-coded determinants: in vitro evidence for a third histocompatibility locus in the mouse. | determinants controlled by the i region of the murine h-2 complex provoked the generation of cytotoxic t lymphocytes (ctl) in both a secondary and primary mixed lymphocyte culture. the stimulating determinants appeared to be controlled by loci within the i-a subregion. the target antigens of the ctl generated were present on both lipopolysaccharide- and concanavalin-induced blast lymphocytes, but were barely detectable on phytohemagglutinin-induced blast cells. the stimulating capacity for ctl i ... | 1975 | 53265 |
| cell-mediated lympholysis of n-(3-nitro-4-hydroxy-5-iodophenylacetyl)-beta-anaylglycylglycyl-modified autologous lymphocytes. effector cell specificity to modified cell surface components controlled by the h-2k and h-2d serological regions of the murine major histocompatibility complex. | splenic lymphocytes from four c57bl/10 congenic mouse strains were sensitized in vitro to n(-3-nitro-4-hydroxy-5-iodophenylacetyl)-beta-alanylglycylglycyl-(n) modified autologous lymphocytes. the effector cells generated after 5 days of culture were assayed on a series of either n-modified phytohemagglutinin-stimulated spleen cells or n-modified tumor cells. the results indicated in all cases that both n modification of the targets and h-2 homology between the modified stimulating and target cel ... | 1976 | 53266 |
| relationships between membrane antigens of human leukemic cells and oncogenic rna virus structural components. | leukemic cells from all human chronic granulocytic leukemia (cgl) and some acute myelomonocytic leukemia (amml) donors are lysed by rabbit antisera to a purified glycoprotein of friend murine leukemia virus (flv gp71) in a microcytotoxicity assay. these antisera are not cytotoxic to cells from patients with acute myelocytic leukemia (aml), acute lymphocytic leukemia (all), chronic lymphocytic leukemia (cll), or to peripheral blood lymphocytes from normal donors. a goat antiserum to gradient puri ... | 1976 | 53269 |
| an effect of tamoxifen (i.c.i. 46,474) on the surface coat of the late preimplantation mouse blastocyst. | the oral adminitration of tamoxifen on days 2,3 and 4 after mating, or on day 2 only, was found to prevent the oestrogen-dependent surface-coat change of the late preimplantation blastocyst in the mouse. it is suggested that his is an antioestrogenic manifestation and that ist is, at least in part, responsible for the drug's known antifertility effect. | 1975 | 53283 |
| letter: cytotoxicity in normal and multiple-sclerosis sera against mouse cells expressing theta-antigen. | | 1975 | 53682 |
| dna-synthesis on giant nuclear rna by amv dna polymerase. | the reverse transcription of pre-mrna isolated from rat liver or mouse ehrlich ascites carcinoma cells with the aid of hot phenol fractionation technique is described. pre-mrna isolated at 85 degrees c is a more active template than the 65 degree c fraction. the addition of oligo(dt) as a primer strongly stimulated the template activity of the 65 degree c fraction. the size of product corresponds to a sedimentation value of 7 s as measured in alkaline sucrose gradient and is essentially less tha ... | 1975 | 53784 |
| immunochemical characterization of murine h-2 controlled ss (serum substance) protein through identification of its human homologue as the fourth component of complement. | the s region of the mouse h-2 complex is genetically defined by a series of alleles, or pseudoalleles, which control the level of a serum globulin, ss, and of its allotypic vairatn, slp. in contrast with the products of other genes in the complex, no homologue of the ss protein has been found in other species, except the rat. in the present study, a component in human plasma was identified which cross-reacts with anti-mouse ss, and which also displays electrophoretic and size similarities to the ... | 1975 | 53836 |
| activation of t and b lymphocytes in vitro: presence of beta2-microblobulin determinants on allogeneic effect factor. | the biologically acitve entity termed allogeneic effect factor, which is produced by alloantigen-activated t cells and has been shown to regulate triggering and differentiation of b lymphocytes in vitro, has previously been demonstrated to possess antigenic determinants coded by genes in the i region of the h-2 gene complex of the mouse. the studies presented here provide evidence that allogeneic effect factor also possesses antigenic determinants identical or cross-reactive with beta2-microglob ... | 1975 | 53839 |
| immunologic cell features in lymphocytic leukemias: a review. | since four years, it has been possible to classify most cases of chronic lymphocytic leukemia (cll) as given to the expansion of abnormal b-lymphocytes. on the contrary, the t-cell class is apparently normal and the t cell extent in cll-peripheral blood can be even greater than normal when taken as absolute value. the clonal nature theory of b-lymphocytes in cll is substantiated by the fact that, in general, in every patient only one ig light chain determinant is present. again, when serum ig mo ... | 1975 | 54182 |
| reaginic antibody formation in the mouse. vi. suppression of ige and igg antibody responses to ovalbumin following the administration of high dose urea-denatured antigen. | | 1975 | 54223 |
| evidence for animal origin of cells and rescued tumour virus from a putative human osteosarcoma cell line. | human osteosarcoma cell lines t 1 and zt 1 were analyzed for host origin. the results indicated that these lines must have been contaminated with rat x mouse hybrid cells. the isolated virus was identified as an avian sarcoma virus belonging to the c subgroup. | 1975 | 54267 |
| allograft tolerance as in immunologically active process: analysis in a restricted h-2 incompatibility due to mutation (m504) in mouse strain b10.d2. | cell-free spleen and liver extracts were used for attempted induction of allograft tolerance across a restricted h-2 barrier due to a chemically induced mutation in the b10.d2 mouse strain. when using mice of the latter strain as donors and the new congenic strain carrying the mutant h-2 hyaplotype as recipients, and with long-term administration of the cell-free antigens in adult life up to 100% yields (according to the dose of antigen) of permanently tolerant animals (particularly males) was o ... | 1975 | 54268 |
| the effects of anti-embryo sera and their localization on the cell surface during mouse preimplantation development. | | 1975 | 54272 |
| species specificity of thromboplastin. a phylogenetic study. | having studied the influence of thromboplastin preparations derived from cold and warm-blooded species on the plasma of 12 vertebrates (carp, frog, turtle, hen, rabbit, rat, mouse, guinea-pig, ground squirrel, dog, sheep and man) we have established that among the species far from each other phylogenetically, the phenomenon of species specificity could be demonstrated in general. it was found that the extrinsic coagulation system measured by quick times can well be activated in every examined sp ... | 1975 | 54309 |
| regulation of helper cell activity by specifically adsorbable t lymphocytes. | mice immunized with soluble proteins such as human serum albumin (hsa) or ovalbumin (oa) develop in their spleens antigen-specific t and b lymphocytes. these populations of lymphocytes can be separated from each other by different means; e.g. treatment with anti-theta-antiserum and complement removes selectively t lymphocytes, whereas passage through glass bead columns coated with mouse immunoglobulin (ig): anti-ig complexes creates a relatively pure population of t lymphocytes. during the cours ... | 1976 | 54390 |
| characteristics of the major internal protein and rna-dependent dna polymerase of bovine leukaemia virus. | a virus designated bovine leukaemia virus (blv), associated with leukaemia in cattle and previously demonstrated to induce the disease in sheep, was purified from chronically infected sheep cell cultures. electrophoretic analysis showed a major protein of mol. wt. about 24,000 (p24) which reacted in gel diffusion and complement-fixation tests with sera from naturally infected cattle, experimentally infected sheep, and guinea pigs immunized with p24. blv p24 has an isoelectric point of 8-6. inter ... | 1975 | 54405 |
| factor supprossing alpha1 fetoprotein production. | the injection of sera of adult mice or of a 20% extract of small and large intestine, liver, kidney, spleen, thymus, and lungs of adult mice suppressed the alpha1 fetoprotein production in syngeneic newborn recipients. extracts of the striated muscles of adult mice had a meager inhibitory effect. extracts of mouse embryonic intestine and those of the while embryo without the gastrointestinal tract had almost negligible amounts of suppressing factor. the possible role of humoral factors regulatin ... | 1975 | 54433 |
| assay in the mouse for delayed-type hypersensitivity to murine leukemia virus. | delayed-type hypersensitivity (dth), assayed by footpad swelling, was induced in 6- to 8-week-old balb/ccr mice immunized with formalin-inactivated, sucrose-banded murine type-c viruses. the dth response was inducible with as little as 11.25 mug sensitizing antigen, was greatest after sc sensitization as compared to im and ip sensitization, and was optimally elicited with a 7-day challenge. a statistical evaluation of the dth assay revealed that the test was consistently reproducible and limited ... | 1975 | 54434 |
| in vitro activation, infectivity, and production of endogenous type-c virus from om rats. | t24c, a continuous cell line derived from the pooled thymic tissue of normal inbred om rats, spontaneously produced type-c virus. the virus genome was expressed cyclically. the amount of rna-dependent dna polymerase (rdp) and the number of 1.14 g dense particles/ml fluctuated simultaneously with cultivation. the released virus, rpt24c, did not infect cell lines from the rat, mouse, dog, or human. t31, also a rat thymus line, during its 2.5 years of cultivation did not produce type-c virus. cocul ... | 1975 | 54437 |
| in vitro inhibition of cell-mediated cytotoxicity against syngeneic friend virus-induced leukemia by immunoregulatory alpha globulin. | immunoregulatory alpha-globulin (ira) derived from normal human plasma decreased cytotoxic reactivity as measured by an in vitro 5-iodo-2'-deoxyridine release assay of immune mouse lymphocytes against the syngeneic friend virus-induced leukemia, fbl-3. this inhibitory effect depended on the dose of ira used and was not due to the cytotoxic effects of ira on the effector cells or target tumor cells. we also found elevated levels of serum alpha-gloubins in fbl-3 tumor-bearing mice as compared to n ... | 1975 | 54438 |
| species and interspecies radioimmunoassays for rat type c virus p30: interviral comparisons and assay of human tumor extracts. | the major internal protein, p30, of rat type c virus (ralv) was purified and utilized to establish intra- and interspecies radioimmunoassays. three rat viruses were compared in homologous and heterologous intraspecies assays with no evidence of type specificity. the only heterologous viruses to give inhibition in these species assays were the feline (felv) and hamster (halv) type c viruses; these reactions were incomplete and required high virus concentrations. an interspecies assay using a goat ... | 1975 | 54444 |
| a rapid and simplified staining of juxtaglomerular granules with aqueous crystal violet. | the method described allows staining of juxtaglomerular granules in a deep purple colour against faint purple background and deep purple erythrocytes. paraffin sections of mouse kidney fixed in 10% formalin buffered with phosphate are brought to water, stained 3 min in 0.01% crystal violet, rinsed in tap water (or stained 3 min in 0.1% crystal violet in nahco3, rinsed in 1% nahco3), blotted, differentiated in a 1 : 1 aniline-xylene mixture, cleared in xylene and mounted in a resin. | 1975 | 54861 |
| partial purification of a serum factor that causes necrosis of tumors. | tumor necrosis can be induced in transplanted mouse methylcholanthrene-induced sarcoma by a tumor necrosis factor in the serum of mice infected with bacillus calmette-guérin and given bacterial endotoxin. sera from normal mice, endotoxin-treated mice, and mice infected with bacillus calmette-guérin do not contain this factor. a 20- to 30-fold purification of the serum factor has been achieved by (nh4)2so4 fractionation, sephadex g-100 and g-200 gel filtration, and preparative polyacrylamide elec ... | 1976 | 54919 |
| structure and evolution of transplantation antigens: partial amino-acid sequences of h-2k and h-2d alloantigens. | techniques for the amino acid sequence analysis of subnanomole quantities of polypeptides have been applied to characterize beta2-microglobulin and transplantation antigens of the mouse isolated from spleen cells by indirect immunoprecipitation. eleven residues were identified throughout the nh2-terminal 27 residues of the beta2-microglobulin; all were identical to residues seen at the corresponding positions of beta2-microglobulins from other species. two k and two d transplantation antigens we ... | 1976 | 54922 |
| cytogenetics analysis of meiotic chromosomes of irradiated mice and their progeny after treatment with streptomycin and dihydrodeoxystreptomycin. | the purpose of this investigation is to find out whether streptomycin and the related compound dihydrodeoxystreptomycin have any mutagenic effect and whether they both are capable of recovering x-ray induced chromosomal translocations in mouse spermatogonia of directly treated animals and their progeny of the first generation. the cytological findings show the absence of any mutagenic effect in animals nonirradiated and treated with streptomycin and dihydrodeoxystreptomycin. the frequency of chr ... | 1975 | 54962 |
| the effect of bleomycin on rapidly proliferating epidermis. a comparative investigation using micro-flow fluorometry, h3tdr incorporation and a stathmokinetic method (colcemid). | at different time intervals after injection of bleomycin (blm) th effect on several kinetic parameters of the hairless mouse epidermis stimulated to proliferate by previous adhesive tape stripping was measured. micro-flow fluorometry was used to determine the relative number of cells in the various phases of the cell cycle (g1, s and g2). tritiated thymidine was used to determine labelling indices and grain counts. colcemid was used to observe the mitotic rate. an initial decrease followed by a ... | 1975 | 54977 |
| properties of mouse leukemia viruses. x. occurrence of viral structural antigens on the cell surface as revealed by a cytotoxicity test. | | 1976 | 54978 |
| properties of mouse leukemia viruses. xi. immunoelectron microscopic studies on viral structural antigens on the cell surface. | | 1976 | 54979 |
| [determination of arbovirus group affiliation in the indirect hemagglutination reaction]. | a dry polyvalent erythrocyte immunoglobulin diagnostic preparation for arboviruses of group b may be prepared to be used in the indirect hemagglutination test. the preparation detects antigens of arboviruses of this group in the tissue culture fluid and in suckling mouse brain tissue treated with sucrose-acetone. the test with group b arbovirus antigens is inhibited specifically by the homologous polyvalent immune gamma globulin. inhibition of hemagglutination by species-specific immune globulin ... | 1975 | 54999 |
| embryology of the epidermis: ultrastructural aspects. iii. maturation and primary appearance of dendritic cells in the mouse with mammalian comparisons. | the epidermis of mice ranging in age from prenatal day 17 through postnatal day 4 and, in addition, postnatal day 18, was studied with the electron microscope. in the 17 day fetus, the periderm may or may not be present and a stratum corneum is developing in the latter case. the cells of the strata spinosum and granulosum contain much glycogen and many keratinosomes and homogeneous keratohyalin granules which appear to line up in a row near the distal cell membranes, fuse and form keratinized ce ... | 1975 | 55035 |
| cytogenetics of the mouse. | | 1975 | 55096 |
| studies of "potentially lethal damage" in emt6 mouse tumour cells treated with bleomycin either in vitro or in vivo. | studies have been carried out into the effect usually referred to as "repair to potentially lethal damage" following the treatment of cells with bleomycin. in vitro, increased survival was seen with delayed subculture of cells in both exponential phase and plateau phase. it was unimportant whether the medium present during the delay period had been previously used to support cell growth. exposure of cells growing as a solid tumour in vivo to bleomycin (4 mg/kg), gave a surviving fraction of 2 x ... | 1975 | 55271 |
| [the effect of heparin and a human heparin precipitable plasma fraction on antibody producing cells in vitro]. | a study was made of the effect of heparin and heparin-precipitable fractions of human blood plasms (hpf) on the capacity of the cells of mouse spleen immunized with sheep erythrocytes to plaque-formation in vitro. it was found that heparin and hpf produced an inhibitory effect on the reaction of plaque formation as a result of interaction with lymphocytes. it is supposed that a possible point of application of heparin and hpf action are surface cell membranes of the antibody-forming cells. | 1976 | 55280 |
| light and electron microscopic observations on the anterograde transport of horseradish peroxidase in the optic pathway in the mouse and rat. | | 1976 | 55292 |
| [classification by the golgi technique of several categories of neurons in the mouse paraventricular nucleus]. | using the rapid golgi technique four types of neurons have been observed in the paraventricular nucleus : magnocellular neurosecretory neurons, parvocellular neurons with "extrahypophyseal" axon, parvocellular neurons with recurrent axon (possibly inhibitory interneurons) and neurons of reticular type. | 1975 | 55295 |
| experimental infection of a cat kidney cell line with the mouse mammary tumor virus. | a cat kidney cell line, crfk-f2, was successfully inoculated in suspension and in monolayer culture with a purified mouse mammary tumor virus derived from riii milk. the virus produced by the infected cells was identified by immunogluorescence, electron microscopy, and rna-directed dna polymerase assays; it was a b-type virion that did not cross-react with mouse or feline leukemia-sarcoma viruses, had spikes on its envelope, and had a rna-directed dna polymerase reaction that was typical of mous ... | 1976 | 55305 |
| species-specific tissue antigens. iii. immunological relationships of enzymic antigens in various species. | immunological relationships have been investigated with acid and alkaline phosphatases, cystine aminopeptidase, beta-acetylglucosaminidase, beta-glucuronidase, catalase and l-glutamate dehydrogenase of human, monkey, mouse, rat, rabbit, dog, cattle, sheep, cat, pig, guinea-pig and chicken organ extracts by means of immunodiffusion and immunoelectrophoresis. extensive cross-reactions among the antigens of most of the enzymes were observed. however, enzymic proteins of acid and alkaline phosphatas ... | 1975 | 55325 |
| cell-mediated immune response in mice treated with steroidal contraceptives. | the splenomegaly assay (simonsen, 1962) was standardized using different strains of rats and mice. male wistar rat (donor)-female swiss mouse (host) was found to be the suitable combination that could be employed in subsequent experiments to study the potential of contraceptive steroids to alter cmir. the index of splenomegaly appeared to increase in case of mice treated with combination oral contraceptives (ovulen, ovral or enovid). the differences observed, however, neared significance only in ... | 1975 | 55327 |
| the fluorescence localization of mouse satellite dna in interphase nuclei. | nuclei from various mouse tissues exhibit a pattern of fluorescence characteristic of the cell type when stained with the fluorescent compound hoechst 33258. when such preparations are hybridized in situ with 3h-rna complementary to the a-t rich satellite of mouse, it is clearly seen that only the fluorescent regions of the nuclei contain the satellite dna. thus hoechst 33258 allows the precise localization of satellite dna at all stages of the mouse cell cycle. | 1976 | 55335 |
| a comparison of four quantitative cytochemical methods directed toward demonstration of dna. | populations of nuclei isolated from mouse brain tissue were stained by the following cytochemical methods considered stoichiometric for dna: (1) the feulgen reaction; (2) gallocyanin-chromalum after rnase; (3) ph 4.0 methylene blue after rnase; and (4) methyl green used in the presence of 2m magnesium chloride. replicate preparations to be stained with gallocyanin-chromalum, methylene blue, and methyl green were acetylated prior to staining. all of these groups were examined by high-resolution s ... | 1975 | 55403 |
| identification of a macrophage-specific cell surface antigen. | a mouse-specific macrophage antigen (msma) was identified in np-40 extracts of 125i-radiolabeled mouse preitoneal macrophages by using a rabbit anti-mouse macrophage serum (ams) and sds-polyacrylamide gel electrophoresis. the antigen was shown to have a m.w. of 83,000 daltons and was present on both normal and "activated" peritoneal macrophages. msma was also present on syngeneic adherent spleen cells, allogeneic peritoneal macrophages, a mouse macrophage cell line (p388d1), and exhibited some c ... | 1976 | 55433 |
| properties of antigen-specific suppressive t cell factor in the regulation of antibody response of the mouse. ii. in vitro activity and evidence for the i region gene product. | an antigen-specific suppressive t cell factor, which was extracted from carrier-primed t cells, was further characterized in an in vitro secondary antibody response. the factor was capable of suppressing secondary igg antibody response of primed spleen cells when it was added to the culture together with relevant antigen. the suppressive t cell factor was not released from primed t cells by a short-term culture with antigen, but was kept bound to the membrane of the residual cultured cells, only ... | 1976 | 55443 |
| evidence for more than one ia antigenic specificity on molecules determined by the i-a subregion of the mouse major histocompatibility complex. | ia antigenic specificities determined by the i-a subregion of the mouse major histocompatibility complex have been examined in strain b10.d2 (h-2d), c57bl/10 (h-2b), and in a (c57bl/6xdba/2) hybrid (bdf1; h-2b/d). detergent solubilized, 3h-leucine-labeled antigen preparations were mixed with appropriate alloantisera and precipitation was induced either by addition of goat anti-mouse gamma-globulin or by addition of protein a-bearing staphylococci. sequential precipitation analysis showed that in ... | 1976 | 55444 |
| analysis of cell-surface markers with staphylococcal protein a: fluorescence studies on mammalian lymphoid determinants. | membrane antigens including different classes of immunoglobulins, transplantation antigens, beta2-microglobulin, t lymphocyte specific antigens, and virally determined surface components were investigated using fluorescein-labeled staphylococcal protein a in combination with cytofluorometric studies. lymphocytes of seven species: mouse, rat, guinea pig, pig, cow, monkey, and human, and of ten human lymphoma-derived lines were tested. analysis of the differential expression of surface markers rev ... | 1976 | 55446 |
| detection of alloantigens during preimplantation development and early trophoblast differentiation in the mouse by immunoperoxidase labeling. | an immunoperoxidase-labeling technique allowing visualization of antibody binding to the cell surface at the electron microscopical level has been employed an an analysis of h-2 and non-h-2 alloantigen expression on the early mouse embryo. the presence of non-h-2 antigenic determinants has been confirmed on eight-cell, morula, and blastocyst stages of development. contrary to previous reports, however, low levels of h-2 antigen have also been detected on the blastocyst. this is the earliest stag ... | 1976 | 55451 |
| specific adsorption of igm antibody onto h-2-activated mouse t lymphocytes. | evidence is presented to support the contention that igm demonstrable by surface immunofluorescent staining on h-2-activated t cells represents specifically adsorbed b-cell-derived alloantibody. t cells activated to h-2 determinants expressed surface igm only when the progenitor cell populations contained b lymphocytes. igm was not detected on t cells activated to determinants which fail to stimulate alloantibody production (e.g., m-locus determinants). in addition, igm-negative h-2 activated t ... | 1976 | 55453 |
| a single genetic element in h-2k affects mouse t-cell antiviral function in poxvirus infection. | cell transfer experiments using mice with recombinant h-2 haplotypes were used to map the h-2 regions which must be shared by ectromelia-immune t-cell donors and virus-infected recipients for transfer of virus clearance mechanisms in the spleen. k- or d-region genes were necessary and sufficient; i-region genes were not involved. the remainder of the mouse genome could be varied widely without impairing the efficacy of t-cell antiviral function, provided either a k or a d region was shared in t ... | 1976 | 55454 |
| antigen-induced aggregation and modulation of receptors on hapten-specific b lymphocytes. | mouse spleen cells were subjected to a fractionation procedure designed to enrich for 4-hydroxy-3-iodo-5-nitro-phenylacetyl (nip)- or dnp-specific b lymphocytes, which depended on adherence of specific cells to a layer of hapten-gelatin at 4 degrees c, recovery of bound cells by melting, and digestion of adherent antigen by collagenase. a population of cells resulted which contained 90% typical b cells and 37% of cells capable of binding a fluorescent, haptenated polymeric protein. fractionated ... | 1976 | 55458 |
| structural characteristics of the alloantigens determined by the major histocompatibility complex of the guinea pig. | the gpla b and ia (i region-associated) antigens are the products of genes found in the guinea pig major histocompatibility complex. because of their importance in immune response phenomena, a structural study of these antigens was undertaken. [3h]leucine and [3h]fucose were internally incorporated into guinea pig lymph node cells. the gpla b and ia antigens were solubilized by the nonionic detergent nonidet p-40, purified by affinity chromatography using an adsorbent column of lentil lectin, is ... | 1976 | 55459 |
| enterotoxin testing of escherichia coli causing epidemic infantile enteritis in the u.k. | three test systems were used to study enterotoxin production by epidemic strains of escherichia coli from cases of infantile enteritis in well-documented outbreaks in the u.k. the tests used were the y1-mouse-adrenal-cell test and the chinese-hamster-ovary-cell (c.h.o.) test for the detection of heat-labile enterotoxin and the infant-mouse test for the detection of heat-stable enterotoxin. all 6 outbreaks had been studied using full serotyping techniques and the results had been published. in ea ... | 1976 | 55904 |
| mouse msv transformed cells resistant to 8-azaguanine. | mouse cells transformed by murine sarcoma virus were made resistant to 8-azaguanine. resistant cells and cell clones isolated from them were deficient in hypoxanthine-guanine phosphoribosyl transferase (hgprt) activity. they did not grow in hatg medium, did not incorporate labeled hypoxanthine, and had negligible hgprt activity. the resistance was genetically stable. the resistant cells were hyperdiploid and contained telocentric chromosomes only. the resistant cells as well as the progenitor ce ... | 1975 | 55981 |
| [the effect of bleomycin on brain tumor cells in vitro (author's transl)]. | in order to evaluate the efficacy of bleomycin (blm) against brain tumor, a study was made on the inhibitory effect of blm on the proliferation of cells derived from 8 cases of astrocytoma and 2 cases of meningioma and the proliferation of ehrlich ascitic, hela and mouse-foot-pad cells of strained epithelial origin. morphological changes induced by blm were also observed with phase contrast microscope and scanning electron microscope. actively growing monolayer cells were exposed to blm whose co ... | 1975 | 55988 |
| immunosuppressive properties of mouse amniotic fluid. | previous reports have suggested that the alpha-fetoprotein present in mouse amniotic fluid is a potent nontoxic immunosuppressant. in the present studies mouse amniotic fluid (1:50) from 9- to 20-day gestations markedly inhibited the in vitro responses of mouse spleen cells to srbc, and spleen cells from nonpregnant females were more affected than were cells from pregnant mice. on the other hand, maf was less effective in depressing antigen- and mitogen-induced proliferation of human blood cells ... | 1976 | 56005 |
| a serum protein associated with chromatin of cultured fibroblasts. | antibodies to chromatin proteins from human and mouse fibroblasts which have been cultured for more than 25 generations with heterologous serum show specificity for a homologous alpha-serum protein. these results indicate that among the chromatin-associated proteins there is one (or more) which has extensive structural similarity to a serum protein. this is the first direct evidence that a serumlike protein or proteins could be chromatin associated in vivo, as has been suggested by experiments s ... | 1976 | 56037 |
| antigenic cross-reactivity among rodent brain tissues and stem cells. | previous studies have shown that antisera prepared in rabbits against mouse brain (ramb) contains activity in vitro against the mouse bone marrow colony-forming unit (cfu) or hematopoietic stem cell. in the present study, in vitro treatment of mouse bone marrow with antisera prepared in rabbits against brain tissue from rats (barb) or hamsters (rahb) also reduced the cfu content of the mouse marrow. prior absorption of the ramb serum with fetal liver tissues from rats or hamsters as well as mice ... | 1976 | 56067 |
| effect of bleomycin on the fine structure of mouse fibroblasts. | asynchronously dividing mouse fibroblasts (l-cells) treated with the antitumour antibiotic bleomycin show various rather specific morphological alterations. many of the cells exposed to bleomycin assume a more or less epitheloid cell shape and are larger than untreated cells; in addition to an increase in nuclear size these cells often contain multiple nuclei. in most of the cells nuclei show an almost complete loss of peripheral condensed chromatin. the nucleolar hypertrophy initially observed ... | 1976 | 56093 |
| [the protective effect of hydroxyethylstarch (hes) in the cryoconservation of bone marrow of the mouse and of man (author's transl)]. | the cryoprotective effect of hydroxyethylstarch has been tested in mice and in man using the spleen colony technique (cfu-s test) or the agar colony technique (cfu-c test) as viability assays for the haemopoietic stem cell. in mice we found a mean cfu-s recovery of 88.7%+/-3.9% when a rapid, and 77.8%+/-3.2% when a slow, stepwise dilution method was applied to remove the protective medium (17.5-20% hydroxyethylstarch+20% calf serum) after thawing. in man the rapid dilution after thawing gave a m ... | 1975 | 56144 |
| rabbit collagenase. immunological identity of the enzymes released from cells and tissues in normal and pathological conditions. | 1. the immunological cross-reactivity between rabbit collagenases from a variety of normal and pathological sources was examined. the specific antibody raised against collagenase secreted from normal rabbit synovial fibroblasts gave reactions of complete identity with collagenases secreted from fibroblasts derived from rabbit skin, and from synovium from experimentally arthritic rabbits. 2. the rabbit fibroblast collagenase was immunologically identical with collagenases obtained from the organ ... | 1975 | 56176 |
| proceedings: modification of the response of mouse skin to x-irradiation by bleomycin treatment. | | 1975 | 56192 |
| autogenous immunity to endogenous rna tumor virus: humoral immune response to virus envelope antigens. | autogenous immune sera from several strains of mice have been examined for type-, group-, or interspecies-specific reactivities against leukemia virus envelope antigens and virus-induced cell surface proteins. the natural antibody of these test sera react with gp69/71, gp43, and p15 structural components on murine leukemia viruses including akr, friend, rauscher, moloney, and xenotropic balb:virus-2. furthermore, comparable radioimmune titration curves are obtained when these viruses are used in ... | 1976 | 56222 |
| cell-mediated immunity to leukemia virus- and tumor-associated antigens in mice. | cell-mediated immune reactions appear to play an important role in resistance against growth of leukemia cells in mice. possible mechanisms for in vivo protection in two tumor systems are discussed. these tumor models, which are a friend leukemia virus-induced transplantable tumor, fbl-3, and primary murine sarcoma virus (msv) -induced tumors, are strongly antigenic; under some conditions, tumors regress completely. in mice with regressing fbl-3 tumors, cell-mediated cytotoxicity was measured by ... | 1976 | 56223 |
| comparison of the early chromatin reaction of the mouse thymocytes incubated with phytohemagglutinin and concanavalin a. | the preliminary activation of mouse thymocytes in vitro following exposure to the mitogens phytohemagglutinin and concanavalin a, was compared. for this purpose the ratio of the fluorescence intensity for acridine orange-dna complex at light wave length gamma = 590 nm to the fluorescence intensity of this complex at light wave length gamma = 530 nm was determined. it was shown that 100% of thymocytes showed an increase in chromatin activity under the influence of con a within 15 minutes of incub ... | 1976 | 56236 |
| the materno-foetal transfer of carrier protein sensitivity in the mouse. | the anti-dinitrophenol (dnp) antibody response was examined in the 18-week-old offspring of female mice primed to bovine serum albumin (bsa) and in control unprimed animals. comparisons were made of the response to dnp-bsa, dnp-chicken gamma-globulin (cgg) and dnp-purified protein derivative (ppd) after all offspring had been primed with dnp-cgg at 12 weeks of age. an enhanced anti-dnp antibody response was found to dnp-bsa in mice whose mothers had received bsa. it is suggested that this phenom ... | 1976 | 56311 |
| the t lymphocyte surface in development. a study of the electrokinetic, antigenic and ultrastructural properties of t lymphocytes in mouse thymus and lymph nodes. | | 1975 | 56320 |
| quantitative fluorescence spectrophotometry of acridine orange-stained unfixed cells. potential for automated detection of human uterine cancer. | after staining with acridine orange (ao), the nuclei of unfixed cells from the human female genital tract exhibited the same fluorescence behavior previously observed for human and murine leukocytes and mouse ascites tumor cells. with staining conditions chosen to assure saturation of the green-fluorescing ao-nucleic acid complex in normal cells, corrected fluorescence emission spectra were recorded from the entire nucleus of 341 cells taken from 32 normal and 28 abnormal patients. intensity of ... | 1976 | 56389 |
| resistance to tumor growth mediated by listeria monocytogenes. destruction of experimental malignant melanoma by lm-activated peritoneal and lymphoid cells. | a murine experimental model of nonspecific tumor destruction mediated by cells activated by listeria monocytogenes (lm) is described. b16 melanoma growth is prevented or suppressed in the syngeneic host when tumor cells are inoculated in contact with viable lm. in vitro, cultured b16 cells are destroyed by lm immune peritoneal or splenic cells in the presence of the bacterial antigen(s). activation of lm immune cells in vitro is immunologically specific. replacement of lm by sheep red blood cell ... | 1976 | 56396 |
| a new alloantigenic system associated with the mls locus in the mouse. | an antiserum prepared by injecting c3h/hej mice with cba/j tissue has been shown to react with cell surface components that are not part of any previously described system of serologically detectable alloantigens. the antiserum, which is designated ast-101, acts selectively in cytotoxic tests carried out with lymphoid cells, killing b cells, but not t cells. phagocytic cells found in peritoneal exudates are also killed by ast-101 and complement in vitro; the sensitivity of other cell types has n ... | 1976 | 56400 |
| motheaten, an immunodeficient mutant of the mouse. ii. depressed immune competence and elevated serum immunoglobulins. | mice homozygous for the recessive mutation motheaten (me) are deficient in capacity for immune response but show an elevated level of serum immunoglobulins. in comparison to spleen cells from normal sibs, spleen cells from me/me mice have a severely depressed 19s pfc response to srbc. in the gvh assay, spleen and thymus cells from motheaten donors caused significantly weaker reactions than like cells from normal sibs. serum electrophoretic patterns of motheaten mice showed increased levels of al ... | 1976 | 56406 |
| effects of sodium periodate modification of lymphocytes on the sensitization and lytic phases of t cell-mediated lympholysis. | sensitization of mouse splenic lymphocytes in vitro with sodium borohydride, suggesting that the biologic effects of sodium periodate are-treated autologous spleen cells stimulated a one-way mixed lymphocyte reaction and led to the generation of thymus-derived cytotoxic effector cells. these effectors were capable of lysing in 4 hr periodate-treated syngeneic and, to a lesser extent, periodate-treated allogeneic target cells. these results suggest that sensitization by periodate-treated autologo ... | 1976 | 56407 |
| genetic control of the immune response to nuclease. ii. detection of idiotypic determinants by the inhibition of antibody-mediated nuclease inactivation. | the humoral response of mice to staphylococcal nuclease has previously been shown to be controlled genetically by h-2-linked ir gene(s). in order to examine the possible contributions of variable region immunoglobulin genes to this genetic control, we have developed a system for the detection of idiotypic determinants on anti-nuclease immunoglobulin molecules. antisera to nuclease were raised in two high responder strains, a/j and sjl. the corresponding antibodies were purified by affinity chrom ... | 1976 | 56408 |