| ovulation timing by a radioreceptor assay for human luteinizing hormone. | since predetermination of ovulation would be helpful in treatment of sterility, a quick, sensitive, and specific radioreceptor assay (rra) for measurement of actual lh concentrations in human serum has been developed. using partially purified membrane receptors from bovine testes, our assay system enabled precise measurement of lh within 4 hours. the detection limit of the present method is 0.78 ng ler 960/ml serum. the method was used to detect ovulation during four observation cycles each in e ... | 1977 | 24601 |
| environment of tryptophan residues in alpha-lactalbumin. | | 1978 | 24620 |
| decreased hyaluronic acid synthesis, a sensitive indicator of cortisol action on fibroblast. | | 1978 | 24703 |
| x-ray diffraction studies of the corneal stroma. | | 1978 | 24753 |
| lh-rh (author's transl). | | 1978 | 24760 |
| role of anions in parathyroid hormone release from dispersed bovine parathyroid cells. | it is known that permeant anions are required for the release of epinephrine from isolated chromaffin granules and of serotonin from intact platelets. we have now investigated the role of anions in the release of a polypeptide hormone, parathyroid hormone, from dispersed bovine parathyroid cells. the release is inhibited 60%-80% by decreasing either cl- or oh- and 60%-70% by replacement of nacl with the impermeant anion isethionate. by contrast, substitution of various monovalent cations in the ... | 1978 | 24845 |
| evaluation of metabolic profile tests using a computer. | a programme has been developed for the evaluation of metabolism profile tests in dairy cows by means of the tesla-200 computer in the fortran language. the computer evaluates and plots the metabolism profile from input data punched on tape in the ibm code by the consul-253 electronic organization automatic device. the metabolism profile is represented as a column graph (histogram), the levels of individual parameters being confronted with the reference levels with a 95% confidence interval and t ... | 1977 | 24916 |
| sanitary-bacteriological characteristics of the soil sprinkled with liquid manure ("shirin-suu"). | | 1977 | 24952 |
| application of a tricin-buffered modification of the cmrl-1969 medium in cell and virus cultivation (author's transl). | cmrl-1969 medium (healy et al., 1971) was modified by using 0.02 molar n-tris-(hydroxymethyl)-methyl-glycin (= tricin) instead of bicarbonate as the buffer substance. several permanent cell lines and primary cell cultures did not show growth differences in the two medium variants. like other non volatile buffers tricin abolishes the initial increase of the ph in freshly split or newly fed closed vessel cultures, but in 0.02 m concentration maintains the same buffering capacity of the medium as c ... | 1977 | 24962 |
| responses of anopheles minimus to ddt residual spraying in a cleared forested foothill area in central thailand. | anopheles balabacensis balabacensis and anopheles minimus are the main malaria vectors in thailand. in a cleared forested foothill area in the central part of the country a. minimus was the most prevalent anopheline species found, only 6 specimens of a. b. balabacensis being collected over a 3-year period. cattle were scarce in the area, tractors being largely used for working in the fields. this situation contributed to high man-vector contact. a minimus occurred throughout the year, with a maj ... | 1978 | 25000 |
| metabolic changes associated with intake by cows of complete diets containing straw and concentrates in different proportions. | 1. pelleted diets containing concentrates and 0, 200, 400 or 600 g chopped straw/kg were fed ad lib, for 5 h daily to four cows. concentrations of various energy-yielding metabolites were measured in samples of rumen fluid and jugular blood taken before feeding and at intervals after food was offered. 2. after feeding, the ph of rumen fluid decreased rapidly and the total concentration of volatile fatty acids (vfa) increased; the changes were greatest in the diet containing no roughage. the chan ... | 1978 | 25080 |
| changes in structure and hydrophobic surface properties of beta-lactoglobulin determined by partition in aqueous two-phase polymeric systems. | the non-polar surface properties of beta-lactoglobulin and especially its interaction with poly(ethylene glycol)-bound palmitate has been studied as a function of ph, temperature and protein concentration. the maximum interaction between beta-lactoglobulin and polymer-bound palmitate occurs at ph 4.3 and ph 7.8. the change in conformation of beta-lactoglobulin around ph 7.5 seems to involve exposure of apolar amino acids to the solvent which results in an increased affinity for hydrocarbons. thi ... | 1978 | 25088 |
| physicochemical properties of cartilage proteoglycans extracted by lanthanum chloride. | bovine nasal cartilage was extracted with 0.5 m lacl3 and the extract then diluted with nine volumes of water. the resulting precipitated (placl3) contained the proteoglycan subunits, together with minor protein components, but was essentially free from hyaluronic acid. the properties of placl3 were investigated by chemical analysis, electrophoresis, viscometry and analytical ultracentrifugation, and the results compared with those for proteoglycan obtained by caesium chloride density gradient c ... | 1978 | 25090 |
| identity of the major protein in 'native' glial fibrillary acidic protein preparation with tubulin. | | 1978 | 25124 |
| cytochemical localization of glucose-6-phosphate dehydrogenase activity in human spermatozoa--ii. significance of the coenzyme. | | 1977 | 25136 |
| purification and characterization of the two molecular forms of membrane acid protease from aspergillus oryzae. | two forms (m1 and m2) of the membrane-bound acid protease of aspergillus oryzae have been purified by extraction with triton x-100, washing with cold acetone, and repeated gel filtration on bio-gel a-15 m in the presence and absence of triton x-100. the purified membrane enzymes, m1 and m2, moved as a single band in acrylamide gel electrophoresis and had apparent molecular weights of 150 000 and 60 000, respectively, as estimated by sodium dodecyl sulfate/acrylamide gel electrophoresis. these tw ... | 1978 | 25177 |
| l-glutamate dehydrogenase. the catalytic properties of the mercurial-activated enzyme. | | 1978 | 25185 |
| interactions between effector cell activity and lymphokines: implications for recovery from herpesvirus infections. | the destruction of herpesvirus-infected target cells by antibody-dependent and direct cell cytotoxicity was enhanced by the presence of bovine lymphokine-containing preparations. to relate these effects to possible in vivo mechanisms of recovery, several in vitro approaches were used to measure the effects of lymphokine-containing preparations on controlling viral spread. in the first approach it was shown that in the presence of lymphokines, virus-infected cells could be killed earlier in the r ... | 1978 | 25244 |
| synthesis and biological activity of ovine beta-lipotropin-(41--91)-henkaipentekontapeptide. | the 51-residue peptide ovine beta-lipotropin-(41--91) has been synthesized by the solid-phase method in about 5% overall yield. the synthetic product was characterized by partition chromatography on agarose gel, thin-layer chromatography in two solvent systems, paper electrophoresis at two ph values, polyacrylamide gel electrophoresis, amino acid analyses of acid and enzymic hydrolysates, and bioassay for lipolytic and melanotropic activities. the synthetic peptide is about 5.4 times as active o ... | 1978 | 25245 |
| intracellular serine protease of bacillus subtilis: sequence homology with extracellular subtilisins. | intracellular serine protease was isolated from stationary-grown bacillus subtilis a-50 cells and purified to homogeneity. the molecular weight of the enzyme is 31,000 +/- 1,000, with an isoelectric point of 4.3. its amino acid composition is characteristically enriched in glutamic acid content, differing from that of extra-cellular subtilisins. the enzyme is completely inhibited with phenylmethylsulfonyl fluoride and ethylenediaminetetraacetic acid. intracellular protease possesses negligible a ... | 1978 | 25266 |
| the role of ca2+ on ph-induced hydrodynamic changes of bovine pancreatic deoxyribonuclease a. | dnase a studied by gel filtration on sephadex g-100 at ph 7.4 in 40 mm tris-hcl buffer, behaves hydrodynamically as a spherical monomeric macromolecule of around 31,000 molecular weight, with a stokes radius = 24.7 a, f/fo = 1.19, and d20,w = 8.69. similar results were obtained by analytical dialysis using zinc chloride-modified cellophane membranes. the elution volume of dnase a decreases as the ph increases between ph 4.7 and ph 9.5. this effect has been attributed to a change in the tridimens ... | 1978 | 25282 |
| the different effects of fe(ii)- and fe(iii)-ions on the hyaluronic acid of the vitreous body (author's transl). | fe(ii)-ions reduce the viscosity of a hyaluronic acid solution but do not form a precipitate. fe(iii)-ions only slightly lower the viscosity of hyaluronic acid solutions but cause a brown, water-insoluble iron-hyaluronic acid precipitate. after adding ascorbic acid--concentrate the ph-value as in vitreous body--to the reaction solution, fe(iii)-ions reduce the viscosity too. the iron-hyalurnic acid complex is also destroyed by ascorbate. therefore, the ascorbate of the vitreous humor plays an im ... | 1978 | 25355 |
| regulation of tyrosine hydroxylase from human pheochromocytoma, bovine adrenal and rat striatum. | | 1978 | 25366 |
| base specific fractionation of double stranded dna: affinity chromatography on a novel type of adsorbant. | a material suitable for base-pair-specific "affinity chromatography" of double stranded dna is described. the synthesis of this material involves two successive polymerization reactions yielding solid particles of cross-linked bisacrylamide to which base-pair-specific dyes are covalently attached by spacers of polyacrylamide chains of different length. materials with immobilized a.t-specific malachite green or g.c-specific phenyl neutral red were used for base-pair-specific fractionation of shea ... | 1978 | 25419 |
| use of kefir for stimulation of gastic secretion and acid-formation in patients with pulmonary tuberculosis. | | 1978 | 25435 |
| identification and properties of two methyltransferases in conversion of phosphatidylethanolamine to phosphatidylcholine. | two methyltransferases involved in the methylation of phosphatidylethanolamine to form phosphatidylcholine were demonstrated in a microsomal fraction of bovine adrenal medulla. the first methyltransferase catalyzes the methylation of phosphatidylethanolamine to form phosphatidyl-n-monomethylethanolamine. this enzyme has an optimum ph of 6.5, a low km for s-adenosyl-l-methionine (1.4 micron), and an absolute requirement for mg2+. the second methyltransferase catalyzes the two successive methylati ... | 1978 | 25437 |
| gldh and gamma glutamyl transferase activities in the serum of healthy and liver-fluke infested cattle. | | 1978 | 25485 |
| comparison of cardiovascular responses and left ventricular work during exercise before and after artificial heart implantation. | cardiovascular dynamics and left ventricular work were evaluated in 15 resting, standing, and treadmill-exercised calves before and at periodic intervals after their natural hearts (nh) were replaced with an artificial heart (ah). standing produced increases in heart rate, cardiac output, oxygen uptake, and left ventricular work and decreases in mean aortic pressure and systemic vascular resistance in nh calves which were more marked with exercise. ah calves had higher aortic pressures, cardiac ... | 1978 | 25489 |
| milk fever prevention. | milk fever is a disease of increasing importance. in dairy herds its incidence has doubled since 1950; today about 8 per cent of parturitions in swedish dairy cattle are complicated by milk fever. at the same time, the clinical picture has changed and the effectiveness of calcium therapy has been markedly reduced. thirty to 40 per cent of cows with milk fever need more than one treatment. these trends are obvious in sweden and norway and have been reported from many other parts of the world. how ... | 1978 | 25507 |
| role of neurotransmitters, prostaglandins and glucose on precursor incorporation into the rna of thyroid slices. | the present studies were performed in order to investigate the role of neurotransmitters, prostaglandins and glucose on 3h uridine incorporation into total rna in beef thyroid slices. carbamylcholine strongly stimulated rna labelling from 3h uridine; atropine abolished this effect. naf, at 1 and 5 mm, progressively increased this parameter while norepinephrine caused a similar effect at 10(-3) but not at 10(-6) m. phentolamine (1 mm) blocked the stimulatory action of tsh; propranolol and atropin ... | 1978 | 25551 |
| the presence of collagenolytic cathepsin in uveal lysosomes of bovine eye. | collagenolytic cathepsin, which can liberate soluble hydroxyproline-containing products from insoluble vitreous collagen with maximum activity at ph 3.5, was biochemically studied in uveal lysosomes of bovine eye. collagen solubilization was proportional to both enzyme concentration and incubation time. when the enzyme was heated, no reaction was observed. collagen solubilization by uveal lysosomal extract was almost unaffected by ca2+ ion, cysteine, beta-mercaptoethanol, and ethylenediaminetetr ... | 1978 | 25592 |
| instability of the bovine mor-2ab (mitochondrial malate: nad oxidoreductase) heterodimeric molecule of heterozygous subjects after isoelectric focusing. | the heterodimeric molecule which is characteristic of animals heterozygous for the mor-2 polymorphism and which is readily seen on enzymograms after electrophoresis is reported to be absent from zymograms performed after isoelectric focusing. a possible explanation is presented based on monomer--dimer equilibrium at ph 8.0--8.5. | 1978 | 25644 |
| proton translocation of the bovine chromaffin-granule membrane. | bovine chromaffin granules were lysed and their membranes resealed to give osmotically sensitive 'ghosts'. these swell in the presence of salts and mgatp. it is shown that this is due to proton entry accompanied by anions. the rate of swelling depends on the anion present, but swelling is not limited to media containing permeant anions. it is quite marked in solutions of sulphates, phosphates and acetates. it is not uncoupler-sensitive, suggesting that at least one component of swelling is due t ... | 1978 | 25653 |
| hydroxytryptamine transport by the bovine chromaffin-granule membrane. | 5-hydroxytryptamine is accumulated by resealed chromaffin-granule 'ghosts' if a ph gradient (acid inside) is imposed across their membranes by preincubating them at low ph. this uptake, like that driven by mgatp, is reserpine- and uncoupler-sensitive. this strongly suggests that catecholamines are taken up by intact granules in response to a ph gradient. in line with this, it is shown that 5-hydroxytryptamine decreases the ph gradient generated in the presence of mgatp, an effect that is inhibit ... | 1978 | 25654 |
| accessibility of sulfhydryl groups to 5,5'-dithiobis-2-nitrobenzoic acid and acid-base properties of bovine and walleye pollock rhodopsin preparations. | both the number of exposed sh-groups and the rate of reaction with 5,5'dithiobis-2-nitrobenzoic acid (dtnb) in walleye pollock and bovine rhodopsin depend on a degree of native structure of the preparation to be investigated. the preparations studied can be arranged in the order of increase of these parameters as follows: ros less than rhodopsin extracted by digitonin less than triton x-100 less than cetyltrimethylammonium bromide (ctab) less than sodium dodecylsulphate (sds). after illumination ... | 1978 | 25681 |
| complement activating factor(s) of trypanosoma lewisi: some physiochemical characteristics of the active components. | of the complement activating factors present in trypanosoma lewisi, the major component, a carbohydrate containing substance was further investigated. this component was found to have a lag time of complete activation of 2 ch50 units of bovine complement of approximately 15 minutes while 1% trypsin (a known activator of complement, used as a control system) was capable of instant consumption of a similar quantity of complement. in addition, the complement activating factor of trypanosomes was ob ... | 1978 | 25701 |
| studies on the purification and properties of a 6.8-s dna polymerase activity found in calf-thymus dna polymerase-alpha fraction. | the heterogeneity of calf thymus dna polymerase-alpha has been further investigated. in particular, an enzyme (enzyme d) which exhibits higher activity on poly(da) . (dt)10 (a:t = 20:1) compared with that on activated dna, has been further purified and its properties compared with two other activities of the dna polymerase-alpha fraction (enzymes a1 and c) which do not show a preference for poly(da) . (dt)10 over activated dna. as with a1 and c, enzyme d was shown to have many of the characteris ... | 1978 | 25765 |
| affinity labelling of the estrogen binding site of glutamate dehydrogenase with iodoacetyldiethylstilbestrol. selective alkylation of cysteine-89. | iodoacetyldiethylstilbestrol was used as an affinity label to alkylate the estrogen binding site of bovine liver glutamate dehydrogenase. this reagent induced inactivation and alkylation of the enzyme. the non-alkylating analogues diethylstilbestrol and estradiol protected the enzyme towards alkylation. the apparent constant of alkylation was of the order of magnitude of i50 for the allosteric inhibition by diethylstilbestrol. these two results suggest that alkylation occurred at the estrogen bi ... | 1978 | 25768 |
| enzymic hydrolysis of 3-acetyl-estrogens or analogues by glutamate dehydrogenase with specific acylation of the estrogen binding site. | | 1978 | 25770 |
| bovine lenticular gamma-glutamylcysteine synthetase: reaction sequence. | the sequence of substrate addition and product release during the reaction catalyzed by gamma-glutamylcysteine synthetase was investigated with purified enzyme from bovine lens. thermal inactivation and kinetic studies suggest that l-glutamate is the first substrate to bind to the enzyme. l-beta-chloroalanine was used as the l-cysteine analogue. utilizing substrate activation and product inhibition studies, the following reaction sequence was determined: l-glutamate binding. atp binding, adp rel ... | 1978 | 25775 |
| neutral cleanup procedure for 2,3,7,8-tetrachlorodibenzo-p-dioxin residues in bovine fat and milk. | a neutral cleanup method for 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) in milk and animal tissue was developed involving solvent extraction and liquid adsorption chromatography on magnesia-celite 545, alumina, and florisil. cleaned up extracts were subjected to dual-ion analysis in a direct probe high resolution mass spectrometer, interfaced to a multi-channel analyzer for signal averaging. calibration experiments were carried out with bovine milk and beef fat samples containing added tcdd. the ... | 1978 | 25875 |
| guanyl nucleotide influences on 3h-ligand binding to alpha-noradrenergic receptors in calf brain membranes. | | 1978 | 25888 |
| enzymatic methylation of carboxyl groups of chromaffin granule membrane proteins. | carboxyl groups of membrane and soluble proteins from bovine adrenal medulla chromaffin granules were enzymatically methylated. the methylated peptides were resolved using gel electrophoresis under acidic conditions in the presence of n-cetylpyridinium chloride. there was a selective methylation of two groups of membrane peptides which did not correspond to any of the chromaffin granule soluble proteins. dopamine beta-hydroxylase, an acidic protein accounting for up to 25% of the membrane protei ... | 1978 | 25891 |
| isolation and characterization of ornithine transcarbamylase from normal human liver. | we report experiments describing the isolation and characterization of ornithine transcarbamylase from normal human liver. our preparative procedure employs initial centrifugation and heat steps, intermediate batch-wise adsorption and desorption from ion exchange resins and column chromatographic elution from hydroxylapatite, and final purification by gel filtration chromatography and glycerol density gradient centrifugation. the enzyme, purified 580-fold in this way, is homogeneous as judged by ... | 1978 | 25896 |
| evidence for stimulation of anion transport in atp-evoked transmitter release from isolated secretory vesicles. | | 1978 | 25897 |
| magnesium-induced inner membrane aggregation in heart mitochondria. | mg(2+) at an optimal concentration of 2mm (ph 6.5) induces large increases (up to 30 percent) in the optical density of bovine heart mitochondria incubated under conditions of low ionic strength (< approx. 0.01). the increases are associated with aggregation (sticking together) of the inner membranes and are little affected by changes in the energy status of the mitochondria. virtually all of a number of other polyvalent cations tested and ag(+) induce increases in mitochondrial optical density ... | 1978 | 25900 |
| 5-chloro-2-phenyl-1-benzobthiophene-3-alkanimines, potential antipsychotic agents. | the title compounds (most notably 2a) were synthesized on the basis of the n-methylation hypothesis of schizophrenia. they were evaluated in dopamine and haloperidol receptor assays. the binding characteristics were comparable in some cases to known neuroleptics. | 1978 | 25967 |
| the binding of intravenous and oral biliary contrast agents to human and bovine serum albumin. | the binding of two homologous series of oral and intravenous biliary contrast agents to human and bovine serum albumin was investigated using the gel filtration technique. all intravenous compounds are bound to human serum albumin via one high affinity and several low affinity binding sites. within the concentration range investigated, about 3--5 high affinity binding sites for the oral compounds were found on human serum albumin. in general, the intravenous compounds have a greater affinity for ... | 1978 | 26028 |
| nucleosome structure. | electron microscopic and biochemical results are presented supporting the following conclusions: (1) two molecules of each histone h2a, h2b, h3 and h4 are necessary and sufficient to form a nucleosome with a diameter of 12.5 +/- 1 nm and containing about 200 base pairs of dna. (2) h3 plus h4 alone can compact 129 +/- 8 dna base pairs into a sub-nucleosomal particle with a diameter of 8 +/- 1 nm. in such a particle the dna duplex is under a constraint equivalent to negative superhelicity. (3) chr ... | 1978 | 26066 |
| pseudotype particles of vesicular stomatitis virus with surface antigens of bovine leukaemia virus--vsv (blv) -- as a sensitive probe for detecting antibodies in the sera of spontaneously infected cattle. | phenotypically mixed particles containing the genome of vesicular stomatitis virus (vsv) and envelope antigen corresponding to bovine leukaemia virus (blv) -- the vsv (blv) pseudotypes -- can be employed as a rapid, specific and sensitive probe for detecting blv-neutralizing antibodies in bovine sera. | 1978 | 26209 |
| superoxide dismutase: a direct, continuous linear assay using the oxygen electrode. | | 1978 | 26288 |
| purification and partial characterization of bovine heart mitochondrial pyridine dinucleotide transhydrogenase. | | 1978 | 26313 |
| congenital and developmental anomalies of the genitalia of slaughtered bulls. | the genital tracts of 968 slaughtered bulls (46% of which were young post-puberal animals) were examined for defects of a congenital or developmental nature. the overall occurrence of such lesions was 7%. these comprised persistent penile frenulum (0.5%), hypospadias (0.3%), detached urethral process (0.4%), testicular hypoplasia (0.2%), cryptorchidism (0.6%), mesonephric duct abnormalities (1.1%) and bulbourethral cysts, fusion and aplasia (3.6%). segmental aplasia of the mesonephric duct, not ... | 1978 | 26328 |
| lactate, pyruvate and actual ph values in the venous blood of newborn calves. | | 1978 | 26330 |
| behavior of phact, po2 and pco2 in venous, capillary and arterial blood of newborn calves. | | 1978 | 26331 |
| a technique for the removal of pyroglutamic acid from the amino terminus of proteins using calf liver pyroglutamate amino peptidase. | | 1978 | 26343 |
| spin-label studies of the sulfhydryl environment in bovine plasma albumin. 1. the n--f transition and acid expansion. | the environment of the sulfhydryl group in plasma albumin was previously characterized by employing spin-labels of varying chain lengths (hull, h. h., chang, r., & kaplan, l. j. (1975) biochim. biophys. acta 400, 132). it was established that the sulfhydryl is in a crevice approximately 10 a deep but this crevice was not identified further. we now report the results of titrating albumin through the acidic conformational transitions while monitoring the electron-spin resonance of the bound nitrox ... | 1978 | 26387 |
| spin-label studies on the sulfhydryl environment in bovine plasma albumin. 2. the neutral transition and the a isomer. | since we were able to demonstrate that the sulfhydryl group is located in the crevice which opens during the n--f transition (cornell, c. n., & kaplan, l. j. (1978) biochemistry 17 (preceding paper in this issue), the investigation was extended by characterizing the environment during the n--b transition and in the a isomer. the results indicate that the n--b and n--f transitions are very similar in that the sulfhydryl group moves from a restricted to unhindered environment during both. the use ... | 1978 | 26388 |
| how much is secondary structure responsible for resistance of double-stranded rna to pancreatic ribonuclease a? | 1. double-stranded f2 sus11 or qbeta rnas, resistant to bovine pancreatic rnaase a in 0.15 m nacl/0.015 m sodium citrate (ssc), are quickly and completely degraded at 10-fold lower ionic strength (0.1 x ssc) under otherwise similar conditions. at this ionic strength the secondary structure of double-stranded rna is maintained, as judged by the following: (a) the unchanged resistance of double-stranded rna and dna, under similar low ionic strength conditions, to nuclease s1 from aspergillus oryza ... | 1978 | 26405 |
| characterization of guanylate cyclase of rod outer segments of the bovine retina. | guanylate cyclase (gtp pyrophosphate-lyse (cyclizing), ec 4.6.1.2.) of bovine retinal rod outer segments is almost completely particulate, i.e. associated with rod outer segment membranes. in contrast to particulate guanylate cyclase in other tissues, treatment of rod outer segments with triton x-100 does not solublize the enzyme but inhibits it. enzyme activity is dependent on the presence of divalent cation, especially mn2+ with only poor activation by mg2+ (10-fold lower) and no activation se ... | 1978 | 26409 |
| methacrylate gels with epoxide groups as supports for immobilization of enzymes in ph range 3-12. | glycidyl methacrylate gels are carriers suitable for attachment of enzymes and for use in affinity chromatography. experiments on the coupling of glycyl-l-leucine and acetyl-l-leucine to these gels have shown a high ph-dependence of the bond formation between the support and the alpha-amino group (ph optimum 9.7); the coupling reaction between the epoxide group and the carboxyl group is practically ph-independent. serum albumin and trypsin were attached to a greater extent in acidic than in alka ... | 1978 | 26411 |
| purification and properties of acid ribonucleases in human serum and leukocytes. | acid rnase was purified from normal human serum about 2400-fold by chromatography on phosphocellulose and sephadex g-75 and rechromatography on sephadex g-75. assayed with yeast rna as substrate, the enzyme showed the maximal activity at about ph 6.5 with sodium phosphate buffer. the reaction was activated by na+, k+, and spermine, but it was not affected greatly by mg2+, co2+, and edta. ca2+, fe2+, zn2+, and cu2+ inhibited the reaction. among the synthetic substrates examined, the enzyme prefer ... | 1978 | 26464 |
| p-nitrobenzyl p-toluenesulfonyl-l-arginine: a chromogenic substrate for thrombin, plasmin and trypsin. | p-nitrobenzyl p-toluenesulfonyl-l-arginine is hydrolyzed by thrombin, plasmin, and trypsin to p-nitrobenzyl alcohol and tosyl-l-arginine. the absorption of p-nitrobenzyl alcohol formed is measured at 271 nm (amm 8.89). with 0.10 mm of the ester in 0.1 m tris-hcl at ph 8.4 and 30 degrees c, the hydrolysis catalyzed by thrombin, plasmin, and trypsin is linearly proportional to time up to consumption of 60% of the substrate. km is 14 micron and vmax is 0.037 mumol/min/nih unit for bovine thrombin, ... | 1978 | 26627 |
| resistance to fasciola hepatica in cattle. ii. biochemical and morphological observations. | | 1978 | 26701 |
| gamma-glutamyl transpeptidase in lactating mammary secretory tissue of cow and rat. | lactating cow and rat tissues were examined for gamma-glutamyl transpeptidase. the specificity of the enzyme for various amino acid acceptors was determined for mammary tissue. the relative specific activity of gamma-glutamyl transpeptidase was high in cow mammary tissue and lower in rat mammary tissue in relation to the activity in kidney of the respective mammals. however, activity per gram wet weight of mammary tissues appeared equivalent. gamma-glutamyl transpeptidase activity towards specif ... | 1978 | 26704 |
| some pharmacological actions of acetylsecohemicholinium. | the effects of the acetylated derivative of hc-3 (acetylsecohemicholinium; achc-3) have been studied at cholinergic nerve terminals and compared with the effects of the parent compound. achc-3 blocked neuromuscular transmission in nerve-muscle preparations; it was shown to be less effective than hc-3 in producing a pre-junctional block in the rat diaphragm but was more effective than hc-3 in eliciting a post-junctional blocking effect in the chick biventer muscle. on the frog rectus abdominis mu ... | 1978 | 26742 |
| biosynthesis of sialic acids. | | 1978 | 26835 |
| beta-galactosidase from bovine testes. | | 1978 | 26842 |
| aryl-beta-hexosidase from bovine liver. | | 1978 | 26843 |
| regional variations in alpha adrenergic receptor interactions of 3h-dihydroergokryptine in calf brain: implications for a two-site model of alpha receptor function. | | 1978 | 26861 |
| proteins containing reductively aminated disaccharides: chemical and immunochemical characterization. | synthetic glycoproteins can be prepared by reductive amination of proteins and reducing carbohydrates in the presence of sodium cyanoborohydride. the reaction proceeds readily in aqueous solution at ph 6--9 to give high degrees of substitution. the degree of substitution can be determined by amino acid analysis, as the 2 degrees amine linkage formed with the epsilon-amino groups of lysine is stable to acid-catalyzed protein hydrolysis conditions. antisera have been obtained to bovine serum album ... | 1978 | 26926 |
| kinetics of cryanoborohydride reduction of bovine rhodopsin. | | 1978 | 27004 |
| protein inhibitor of cyclic nucleotide phosphodiesterase in the retina in hereditary degeneration. | thermostable protein fraction from retina of rats with hereditary retinal dystrophy (hunter and campbell strains) did not inhibit cyclic nucleotide phosphodiesterase. at the same time an inhibitory component, found in retina of wistar rat, rabbit, frog and lamprey, was similar to the component from bovine retina. quantity of the inhibitory component in normal rat retina decreased considerably within postnatal period (12 days--3 months). thermostable proteins, isolated from campbell rat retina, d ... | 1978 | 27006 |
| on the binding of cyclophosphamide and cyclophosphamide-metabolites to serum-albumin (author's transl). | | 1978 | 27021 |
| multiple enzyme forms. | | 1977 | 27023 |
| change in the protein components of milk during the preparation of a sour milk product with an increased biological value and biolact. | | 1978 | 27029 |
| nuclear translocation of catalytic subunits of cytosol camp-dependent protein kinase in the transsynaptic induction of medullary tyrosine hydroxylase. | | 1978 | 27077 |
| properties and isozymes of acid and alkaline phosphatase in cattle testes and endometrium. | the activity of acid phosphatase in the testicular parenchyma of cattle was found to be higher than that in the endometrium. the acid phosphatase of either tissue broke up p-nitrophenylphosphate at a rate twice as high as that reached for di-sodium phenylphosphate. hydrolysis of phenolphthalein diphosphate was slower and that of beta-glycerophosphate very slow. two optimum ph values and two isozymes were recordable from acid phosphatase in testicular supernatant and three optimum ph values as we ... | 1978 | 27159 |
| studies on diarrhea of young calves. 1. quantitative analysis of the gastrointestinal flora in clinically healthy calves and in calves with diarrhea. | quantitative composition of gastro-intestinal-flora was determined in 23 clinically healthy calves and the course of germ spreading was followed up. in comparison with these healthy animals in 25 diarrhoea affected calves at the age of 4 to 17 days there could be observed signigicant differences only in few parts of the gastro-intestinal-tract concerning the quantitative composition of germ flora. only in 3 animals enterotoxin producing e coli strains could be detected. | 1978 | 27162 |
| stabilization of human fibroblast interferon purified on concanavalin a-agarose. | human fibroblast interferon, obtained by chromatography on concanavalin a-agarose, was stable for at least a month in 30--50 per cent ethylene glycol at 4 degrees, --20 degrees, and --70 degrees c. the succinct point of the present finding is that human fibroblast interferon may be stabilized by ethylene glycol alone without the addition of bovine serum albumin and 'back-contamination' of the interferon preparation. | 1978 | 27163 |
| equilibrium binding of coenzymes and substrates to nicotinamide-adenine dinucleotide phosphate-linked isocitrate dehydrogenase from bovine heart mitochondria. | 1. the stoicheiometries and affinities of ligand binding to isocitrate dehydrogenase were studied at ph 7.0, mainly by measuring changes in nadph and protein fluorescence. 2. the affinity of the enzyme for nadph is about 100-fold greater than it is for nadp+ in various buffer/salt solutions, and the affinities for both coenzymes are decreased by mg2+, phosphate and increase in ionic strength. 3. the maximum binding capacity of the dimeric enzyme for nadph, from coenzyme fluorescence and protein- ... | 1978 | 27170 |
| transient kinetics of nicotinamide-adenine dinucleotide phosphate-linked isocitrate dehydrogenase from bovine heart mitochondria. | pre-steady-state studies of the isocitrate dehydrogenase reaction show that the rate constant for the hydride-transfer step is above 990s-1, and that both subunits of the enzyme are simulataneously active. after the fast formation of nadph in amounts equivalent to the enzyme subunit concentration, the rate of nadph formation is equal to the steady-state rate if the enzyme has been preincubated with isocitrate and mg2+. if the enzyme has been preincubated with nadp+ and mg2+, in 0.05 m-triethanol ... | 1978 | 27171 |
| a neutral soluble high molecular weight keratin from epidermis. | | 1978 | 27174 |
| superoxide anion as a cofactor of dopamine-beta-hydrxylase. | | 1978 | 27181 |
| superoxide dismutase activity of cytochrome oxidase. | | 1978 | 27184 |
| relaxation of solvent protons by cobalt bovine carbonic anhydrase. | | 1978 | 27188 |
| evidence for phosphorylation of bovine adrenal tyrosine hydroxylase by cyclic amp-dependent protein kinase. | | 1978 | 27192 |
| transglutaminase-catalyzed insertion of a fluorescent probe into the protease-sensitive region of rhodopsin. | | 1978 | 27209 |
| lipid metabolism by the gall-bladder. ii. the in vitro conversion of lysophosphatidylcholine to phosphatidylcholine. | lysophosphatidylcholine acyltransferase, which catalyzes the acylation of lysophosphatidylcholine with fatty acid coenzyme a to form phosphatidylcholine, was assayed in gall-bladder mucosa. in guinea pig gall-bladder the activity parallels that of the microsomal enzyme, glucose-6-phosphatase with 3--4-fold enrichment of the activity in the microsomes. studies with saturated and unsaturated substrates demonstrated highest activity when oleoyl coenzyme a and palmitoyl lysophosphatidylcholine were ... | 1978 | 27225 |
| effect of ph on bovine liver catalase as determined by electron paramagnetic resonance. | two major rhombic high-spin ferric heme signals are observed during the ph titration of bovine liver catalase. the less rhombic signal if dominant above ph 6.0 and the more rhombic signal below ph 6.0. ethanol in high concentration enhances the relative intensity of the less rhombic signal. these data demonstrate the sensitivitiy of the ligand field to changes in catalase solvent and, furthermore, suggest that both rhombic configuration posses identical spectral and catalytic properties. | 1978 | 27228 |
| the cleavage and adsorption of parathyroid hormone at high dilution: implications for receptor binding studies. | like other polypeptide hormones, purified intact parathyroid hormone (1-84)parathyroid hormone is notoriously unstable and is subject to large adsorptive losses in routine laboratory manipulation. the present studies were undertaken with 125i-labeled hormone to quantitate the problem and to develop preventative measures, particularly with concentrations of physiological interest, 1 . 10(-10) m. it was found that spontaneous cleavage of the hormone takes place upon its incubation in air or oxygen ... | 1978 | 27231 |
| properties of partially purified bovine brain dopamine beta-hydroxylase. | dopamine beta-hydroxylase has been partially purified from bovine brain. a 140-fold purification factor was achieved using solubilization with triton x-100, ammonium sulphate fractionation between 20-50 per cent saturation, affinity chromatography on concanavalin a-sepharose 4 b and then filtration through sephadex g200. the specific activity at the end was 51 nmoles/h/mg protein. the majority of endogenous inhibitors were lost. immunological studies, kinetic studies, studies on the interaction ... | 1978 | 27241 |
| properties of cyclic nucleotide phosphodiesterase from lingual taste papillae. | phosphodiesterase activity is estimated in extracts and partially purified preparations from functionally different parts of bovine tongue. the enzyme activity varied from 4.0 to 10.4 nmole/mg of protein/min. properties of phosphodiesterase from circumvallate papillae are studied, the ph optimum being 8.0--8.5, km for camp--1.5.10(-4) m and for cgmp--6.5.10(-5) m. the enzyme activity did not change after the treatment with trypsin, protamine sulphate (0.01--1.0%), heparin (0.01--1.0) and taste a ... | 1978 | 27246 |
| neurochemical anatomy of the neuroendocrine hypothalamus. neurochemical anatomy of the hypothalamus. | microdissection techniques for isolated removal of the various regions of the hypothalamus as well as the individual hypothalamic nuclei are detailed. recent development of biochemical microassays have made it possible that the concentrations of neurohormones, neuropeptides, neurotransmitters and their related enzymes could be detected in such a small volume of brain tissue than the hypothalamic nuclei. data available of the hypothalamic distribution of above substances are summarized. the possi ... | 1978 | 27266 |
| the effects of isoprenaline and a new beta-sympathomimetic amine upon spontaneous activity, diastolic depolarization and plateau height in cardiac purkinje fibres. | 1 in spontaneously active purkinje fibres of young cows the dose-response curves of the action of isoprenaline upon different electrophysiological parameters were measured.2 the increase in slope of diastolic depolarization could roughly be described by a one-for-one binding curve with a half maximum effect near 10(-8) m and the increase in the height of the plateau level by a two-for-one binding curve with a half maximum effect near 10(-7) m (-)-isoprenaline.3 these dose-response curves were si ... | 1978 | 27280 |
| studies related to antitumor antibiotics. part xiv. reactions of mitomycin b with dna. | | 1978 | 27289 |
| calcium-binding properties of cardiac and skeletal troponin c as determined by circular dichroism and ultraviolet difference spectroscopy. | calcium titration of the conformational change in cardiac and skeletal troponin c (tn-c) was followed by circular dichroism (cd) at ph values in the range from 5.2 to 7.4. computer analysis was used to resolve the contributions from the different classes of ca2+ -binding sites. at ph 6.94 in skeletal tn-c, apparent affinity constants for calcium of 1.8 x 10(7) and 4.5 x 10(5) m-1 were determined for the two classes of binding sites. the more sophisticated computer analysis of the data has reveal ... | 1978 | 27291 |
| isoelectric focusing of bull seminal plasma proteins. | | 1978 | 27401 |
| porphyrin biosynthesis-immobilized enzymes and ligands-x. a novel approach to the study of the relationship between the quaternary structure of aminolaevulinate dehydratase and its activity. | | 1978 | 27405 |
| gamma-glutamyltransferase in human diploid fibroblasts and other mammalian cells. | gamma-glutamyltransferase was determined in wi-38 human diploid fibroblasts and compared to enzyme levels determined in several other mammalian cell lines including: fibroblast-like cells from human skin, tibia and foreskin; epithelial-like cells from human, bovine and monkey kidney; and transformed cells (chinese hamster ovary, hela s3 and sv-40 transformed wi-38). transformed cells had the lowest activity found followed in increasing order by fibroblasts, human and bovine epithelial cells and ... | 1978 | 27446 |