| serpin-like properties of alpha1-antitrypsin portland towards furin convertase. | recent studies have demonstrated that a serpin variant, alpha1-antitrypsin portland (at-pdx), can inhibit the mammalian convertase furin. here, we examine the mechanism by which this inhibition takes place. we find that furin, which does not belong to the trypsin-like serine protease family, the usual targets of serpins, forms an sds-heat denaturation-resistant complex with at-pdx both in vitro and in vivo. at-pdx inhibited furin with an association rate constant (k(ass)) of 1.5 x 10(6) m(-1) s( ... | 1998 | 9598975 |
| culicoides in relation to transmission of african horse sickness virus in the gambia. | twelve light trap collections made near overnight shelters of horses and donkeys in four villages in the central river division of the gambia captured fourteen species of biting midge of the genus culicoides. five species new to the gambia were identified. this brought the number of recognized species of culicoides (after a revision of c. schultzei) to twenty-nine in the gambia. species known or suspected as vectors of african horse sickness virus (ahsv) and bluetongue virus (btv) comprised 83% ... | 1998 | 9622369 |
| homologous plasma-stored tendon grafting in equids--gross and histomorphological observations. | the efficacy of homologous plasma-stored tendon grafting in the superficial digital flexor tendon severed in the mid-metacarpal region was evaluated in 10 donkeys. experimental tenectomies were performed, followed by grafting using plasma-stored tendon and human amniotic membrane encasement in group i (n=8) but no grafting or encasement in group ii (control) (n=2). gross observations in group i showed no peritendinous adhesions, except in one animal, while control animals showed thick fibrous ad ... | 1998 | 9625461 |
| [the action of the proton pump inhibitor pantoprazol against acetylsalicylic acid-induced gastroduodenopathy in comparison to ranitidine. an endoscopic controlled, double blind comparison]. | in a randomised double-blind parallel study the gastroduodenal tolerability of 300 mg acetylsalicilic acid daily (asa, cas 50-78-2) has been evaluated in the presence of placebo (n = 8), 40 mg pantoprazole (cas 102625-70-7) daily (8 a.m.) (n = 16) and 300 mg ranitidine (cas 66357-35-5) daily (8 a.m.) (n = 16) in healthy volunteers using upper gi-endoscopy. the treatment period lasted 14 days, endoscopic controls were performed at entry and repeated at day 14. at entry, the mean endoscopic score ... | 1998 | 9638315 |
| induction of micronuclei in the domestic cat (felis domesticus) peripheral blood by colchicine and cytosine-arabinoside. | one advantage of bioassays over other kinds of tests is the processing or metabolism of the compound by the organism. in a previous work, in which we reported the number of micronucleated erythrocytes (mne) in the peripheral blood of 35 species, the cat was one of the species with the highest number of micronuclei (mn). cats have a low level of circulating polychromatic erythrocytes (pce), i.e. 0.05%, which is much lower than in the laboratory mouse, but higher than in species such as cows, donk ... | 1998 | 9639702 |
| increased excretion of aromatic amino acid catabolites in animals infected with trypanosoma brucei evansi. | aromatic amino acid catabolism by trypanosoma brucei evansi was investigated in vivo using c3heb/fej mice. the major catabolites detected by gas chromatography in the urines of infected animals were phenylpyruvic acid, 4-hydroxyphenylpyruvic acid, and indole-3-pyruvic acid. identity of each compound was confirmed by gas chromatography-mass spectrometry. concentrations of catabolites in urine of infected mice were correlated with parasitemia and returned to normal following suramin treatment. oth ... | 1998 | 9645841 |
| chromosomal rearrangements in a somali wild ass pedigree, equus africanus somaliensis (perissodactyla, equidae). | chromosome analyses were conducted on 15 animals in a pedigree of somali wild ass, equus africanus somaliensis. g- and c-banded karyotypes are presented for the first time on this endangered species. the diploid number ranged from 62 to 64. numerical chromosomal variation was the result of a centric fission which was accompanied by a heterochromatic deletion. the fission polymorphism involved acrocentric elements 19 and 21 as determined by g-banding. these autosomes are homologous to those invol ... | 1998 | 9678344 |
| [solid-phase immunoenzyme ass for detecting giardia lamblia antigens]. | an elisa sandwich for the detection of giardia lamblia antigens in human faeces was standardized. 175 samples were studied: 77 positive, 61 negative to cysts and/or trophozoites by direct faeces test, and 19 positive to other parasite different from g. lamblia. the sensitivity of the technique was 94.8% and the specificity 98.3%. the method detects an antigen concentration of 31 ng. the procedure is simple, sensitive and specific so, it may be useful for diagnosis and in epidemiological studies. | 1997 | 9685962 |
| pharmacokinetics of sulfonamides and trimethoprim in the donkey (equus asinus). | the body disposition of sulfadimidine (sdm), sulfadiazine (sdz), sulfamethoxypyridazine (smpz) and a trimethoprim-sulfadimethoxine combination (tmp-sdmx) was investigated in the donkey. the four sulfonamides and tmp were injected intravenously at doses of 20 mg/kg (sdm, sdz, smpz), 12.5 mg/kg (sdmx) and 2.5 mg/kg (tmp). the body clearance (clb) of sdz (1.70 +/- 0.14 ml/min/kg) was significantly higher than those of sdm (1.13 +/- 0.18 ml/min/kg), smpz (1.10 +/- 0.09 ml/min/kg) and sdmx (0.75 +/- ... | 1998 | 9697419 |
| a comparative study of mast cells and eosinophil leukocytes in the mammalian testis. | the existence of a physiological integration between the immune and endocrine systems has long been recognized. in spite of the abundant literature data on the presence of cells of the immune system in the testis, mast cells and eosinophil leukocytes have received little attention. we have studied the presence, distribution and numbers of mast cells and eosinophils in the testes of 12 mammalian species. mast cells were frequently found in equine (stallion, ass and mule) and human testis, whereas ... | 1998 | 9697421 |
| hyperlipaemia in a donkey. | | 1998 | 9700398 |
| a case of open multiple fractures of both forearms caused by a donkey bite. | | 1998 | 9707818 |
| an outbreak of equine leukoencephalomalacia at oaxaca, mexico, associated with fumonisin b1. | equine leukoencephalomalacia (elem), swine pulmonary oedema and human oesophageal cancer have been associated with fumonisine b1 (fb1) ingestion. for the first time in this study it is reported that fb1 was identified as being associated with an outbreak of elem at oaxaca, mexico. symptoms of elem and equine venezuelan encephalitis (eve) are similar and a different diagnosis is obligatory. in the geographical area (oaxaca, mexico) where donkeys died showing a neurological syndrome, 14 corn sampl ... | 1998 | 9719762 |
| [lead in the blood and protoporphyrin levels in erythrocytes in glass workers exposed to lead]. | this paper investigates how the indicators of lead exposure (blood lead-pbb, delta-aminolevulinic acid dehydratase-delta-alad, erythrocyte protoporphyrin-ep), relate to the exposure duration prior to the analyses, alcohol intake, gamma-glutamyltranspherase (ggt), and smoking habits of 25 glass workers exposed to lead oxide over a period between 1988 and 1994. the pbb was analyzed with the et ass method, (alad with standardized european method) while ep with the spectrofluorometric method. the al ... | 1997 | 9721457 |
| immunologic, microscopic, and molecular evidence of encephalitozoon intestinalis (septata intestinalis) infection in mammals other than humans. | encephalitozoon intestinalis (septata intestinalis) is the second most prevalent microsporidian species infecting humans, but it has not been described in other animal species. this investigation examined 10 domestic animal stool samples (8 mammalian, 2 avian) containing spores detected by anti-encephalitozoon monoclonal antibody immunofluorescence (fa). the presence of e. intestinalis but not encephalitozoon hellem or encephalitozoon cuniculi was confirmed in 6 of 8 mammalian stool samples by s ... | 1998 | 9728552 |
| enterococcus asini sp. nov. isolated from the caecum of donkeys (equus asinus). | several gram-positive, non-spore-forming and non-motile bacteria consisting of pairs or chains of cocci were isolated during an investigation of the bacterial flora of the caecum of donkeys. physiological and metabolic data indicated that the strains belong to the genus enterococcus; phenotypic traits of these organisms were not consistent with any of the currently known enterococcus species. 16s rrna gene sequence analysis placed these strains in the genus enterococcus. their closest relatives ... | 1998 | 9731276 |
| compartmentation and kinetics of urea cycle enzymes in porcine enterocytes. | we have recently reported the synthesis of urea from ammonia, glutamine and arginine in enterocytes of postweaning pigs. the present study was conducted to determine the compartmentation and kinetics of urea cycle enzymes in these cells. carbamoyl phosphate synthase i (cps i) and ornithine carbamoyltransferase (oct) were located exclusively in mitochondria, whereas argininosuccinate synthase (ass) and argininosuccinate lyase (asl) were found in the cytosol. arginase isozymes were present in both ... | 1998 | 9734336 |
| the icd in france: money problem or donkey syndrome? | | 1998 | 9744428 |
| helminth levels of working donkeys kept under different management systems in the moretele 1 district of the north-west province, south africa. | in southern africa, where 150,000 working donkeys provide an important alternative to mechanisation in resource-poor communities, very little is known about their helminth status, or about the impact of helminths on their work output. the aim of this study was to investigate the helminth status of working donkeys under different management systems. donkey owners in three different areas (one rural and two semi-rural) of the moretele 1 district of north-west province, south africa, were visited a ... | 1998 | 9746286 |
| [effects of high sodium intake on ventricular remodeling in mice]. | despite extensive research, controversy still exists regarding the role of dietary sodium intake on hypertension and left ventricular (lv) hypertrophy. echocardiography is a powerful tool to assess lv hypertrophy and recent technical developments allow now its use in small animals. | 1998 | 9749140 |
| comparative neurovirulence and tissue tropism of wild-type and attenuated strains of venezuelan equine encephalitis virus administered by aerosol in c3h/hen and balb/c mice. | to assess the potential for aerosol administration of vaccines for venezuelan equine encephalitis virus (vee), we compared the neurovirulence and tissue tropism of the wild-type trinidad donkey (trd) strain to those of the attenuated tc83 and v3526 strains of vee in mice. six to 8-week-old female c3h/hen and balb/c mice were aerosol exposed to one of the three vee strains. three mice of each strain were euthanatized at different times and their tissues were processed and stained using hematoxyli ... | 1998 | 9754544 |
| pedal osteosarcoma in a donkey. | an 18 1/2-year-old castrated male donkey with progressively worsening right forelimb lameness presented with a mass on the distal dorsal aspect of its p3 bone. grossly, the firm, gritty mass was infiltrative, disrupted the contours of the overlying hoof wall, and had mottled and cavitated areas on cut surface. histologically, the growth was composed of densely cellular sheets of mildly pleomorphic mesenchymal cells forming irregularly shaped islands of poorly mineralized osteoid. the neoplastic ... | 1998 | 9754546 |
| beta-amyloid precursor protein and ss-amyloid peptide immunoreactivity in the rat brain after middle cerebral artery occlusion: effect of age. | background and | 1998 | 9756603 |
| pig, donkey and buffalo meat as a source of some coccidian parasites infecting dogs. | experimental infection of dogs with meat samples (oesophagus, heart and diaphragm) from each of 105 pigs, 11 donkeys and 17 egyptian water buffaloes indicated that they contained the infective stages of some coccidian parasites of dogs. the dogs which were fed pig meat shed in their faeces isospora ohioensis, i. canis oocysts and sarcocystis miescheriana sporocysts after prepatent periods of 3-5, 4-7 and 9-10 days, respectively. the dogs which were fed donkey meat excreted only i. ohioensis oocy ... | 1998 | 9760058 |
| codistribution of procathepsin b and mature cathepsin b forms in human prostate tumors detected by confocal and immunofluorescence microscopy. | cathepsin b (cb) is involved in invasion and metastasis of a variety of solid organ tumors, including human prostate cancer. the tertiary structures of the proenzyme and mature forms of cb are related closely, as revealed by crystallographic studies. however, the cellular distributions of the cb forms have not been defined in human prostate and its tumors. our objective was to investigate the distribution and codistribution of cb and procathepsin b (procb) in human prostate tumors. human prostat ... | 1998 | 9776083 |
| the matrix effects on kinetic rate constants of antibody-antigen interactions reflect solvent viscosity. | this study describes the influence of different matrices on two model antibody-antigen interactions; that between beta2microglobulin and anti beta2microglobulin, and that of rabbit anti mouse fc fragment (ramfc) with mouse igg. the matrices investigated were; phosphate-buffered saline ph 7.4 containing 0.05% tween 20 detergent, horse serum, a 50:50 mixture of phosphate-buffered saline/tween 20 and horse serum, and four glycerol solutions of differing concentrations. a recently developed optical ... | 1998 | 9776574 |
| an annotated checklist by genus and species of 93 species level names for 51 recognized species of small strongyles (nematoda: strongyloidea: cyathostominea) of horses, asses and zebras of the world. | the results of an international collaborative effort to prepare a recommended list of scientific names for the small strongyles (nematoda: strongyloidea: cyathostominea) of horses, donkeys and zebras are reported. fifty-one valid species are recognized in 13 genera, including cyathostomum, coronocyclus, cylicodontophorus, cylicocyclus, cylicostephanus, skrjabinodentus, tridentoinfundibulum, petrovinema, poteriostomum, parapoteriostomum, hsiungia, cylindropharynx and caballonema. in addition, 42 ... | 1998 | 9777726 |
| epidemiology of african horse sickness and the role of the zebra in south africa. | zebra are the only equine species native to south africa. these animals roamed over much of the country in the 17th century when horses and donkeys were first imported. the first cases of african horse sickness (ahs) then occurred in the horses of hunters who entered zebra territory. ahs continued to occur on a country-wide basis until the beginning of the 20th century, though the number of outbreaks decreased as the populations of zebra collapsed through overhunting. for most of the 20th centur ... | 1998 | 9785491 |
| serological and virological responses in mules and donkeys following inoculation with african horse sickness virus serotype 4. | two groups, comprising 4 donkeys and 4 mules (group 1) and 4 donkeys and 3 mules (group 2), were used to determine the duration of viraemia and to monitor the development of antibodies following inoculation with african horse sickness virus (ahsv). one group of animals was given a single dose of attenuated ahsv serotype 4 (ahsv 4) vaccine. the second group was inoculated with a virulent field strain of ahsv 4. both groups were subsequently challenged with the virulent field strain of ahsv 4, 51 ... | 1998 | 9785493 |
| donkeys as reservoirs of african horse sickness virus. | investigations have been carried out to elucidate the possible role of the donkey in the epidemiology of african horse sickness (ahs). these studies have shown that despite the absence of pyrexia or other observable clinical signs, donkeys become infected with virulent ahs virus serotype 4 (ahsv 4) and that they develop a viraemia which can persist for at least 12 days, albeit at a comparatively lower titre than that recorded for similarly infected ponies. ahsv 4 showed a similar tissue tropism ... | 1998 | 9785494 |
| clinical, virological and immune responses of normal and immunosuppressed donkeys (equus asinus africanus) after inoculation with african horse sickness virus. | to elucidate the role that donkeys may play in african horse sickness virus (ahsv) persistence during inter-epizootic periods we looked for clinical signs of infection and studied the viraemia and neutralising antibody kinetics in 3 immunocompetent and 3 immunosuppressed donkeys inoculated with ahsv-4. none of the donkeys developed signs of ahs. however infectious ahsv was isolated from the blood of the immunocompetent donkeys for up to 17 days post infection (dpi) and viral antigens were detect ... | 1998 | 9785495 |
| simulation studies of african horse sickness in spain. | factors affecting epidemics of african horse sickness in spain were studied using a mathematical model. the model examined the likelihood of an epidemic after the introduction of the virus, and the effectiveness of vaccination strategies. two host species (horses and donkeys) and one vector species (the biting midge culicoides imicola) were included. a stratified random sampling method (latin hypercube sampling) was used for sensitivity analysis of the likelihood of an epidemic. systematic varia ... | 1998 | 9785500 |
| application of an indirect fluorescent antibody assay for the detection of african horse sickness virus antibodies. | an indirect fluorescent antibody (ifa) technique was used to screen and quantify antibodies against african horse sickness virus (ahsv) in equine sera. results obtained with the ifa assay were compared directly with those obtained with standard complement fixation (cf) and virus neutralisation (vn) tests using horse sera from experimental studies and samples from the field. positive fluorescent antibody titres were detected from as early as 7 days after primary vaccination and persisted for at l ... | 1998 | 9785515 |
| use of reverse transcriptase-polymerase chain reaction (rt-pcr) and dot-blot hybridisation for the detection and identification of african horse sickness virus nucleic acids. | a coupled reverse transcriptase-polymerase chain reaction assay (rt-pcr) for the detection of african horse sickness virus (ahsv) dsrna, has been developed using genome segment 7 as the target template for primers. rna from isolates of all nine ahsv serotypes were readily detected. the potential inhibitory effects of either ethylene diamine tetra acetic acid (edta) or heparin on the rt-pcr were eliminated by washing blood samples before lysis of the red blood cells and storage. there was a close ... | 1998 | 9785517 |
| the evolution of aging: a new approach to an old problem of biology. | most gerontologists believe aging did not evolve, is accidental, and is unrelated to development. the opposite viewpoint is most likely correct. genetic drift occurs in finite populations and leads to homozygosity in multiple-alleled traits. episodic selection events will alter random drift towards homozygosity in alleles that increase fitness with respect to the selection event. aging increases population turnover, which accelerates the benefit of genetic drift. this advantage of aging led to t ... | 1998 | 9792199 |
| immunolocalization of regulatory peptides and 5-ht in bovine male urogenital apparatus. | specimens of testis, excurrent duct including the accessory genital glands and urethra throughout its extension were investigated in adult bovines, in order to immunohistochemically localize both the peptidergic innervation and the epithelial cell types belonging to the diffuse endocrine system (des). immunoreactivities to grp, met- and leu-enkephalins, cgrp, npy, substance p, vip, somatostatin, beta-endorphin and 5-ht antisera were tested by means of a labelled streptavidin-biotin (lsab) method ... | 1998 | 9810501 |
| resistance to alpha/beta interferons correlates with the epizootic and virulence potential of venezuelan equine encephalitis viruses and is determined by the 5' noncoding region and glycoproteins. | we compared the alpha/beta interferon (ifn-alpha/beta) sensitivities of the tc-83 vaccine strain and 24 enzootic and epizootic venezuelan equine encephalitis (vee) isolates. the ifn-resistant or -sensitive phenotype correlated well with epizootic or enzootic potential. ifn-alpha/beta resistance of trinidad donkey (trd) virus correlated with virulence determinants in the 5' noncoding region and glycoproteins. infection of mice lacking a functional ifn system with the ifn-sensitive tc-83 virus res ... | 1998 | 9811777 |
| argininosuccinate synthetase: localization in astrocytes and role in the production of glial nitric oxide. | an antiserum raised against the peptide representing the partial sequence 196-222 of mouse liver argininosuccinate synthetase (ass) was used to detect and localize the enzyme in cells of neural primary cultures. no ass immunoreactivity was detected by western blotting in homogenates of mouse pure astroglial cultures and rat astroglia-rich cultures. however, when the cultures had been treated with bacterial lipopolysaccharide, interferon-gamma, or a combination of both, ass immunoreactivity was d ... | 1998 | 9814823 |
| the guttural pouch is not present in the white rhinoceros (ceratotherium simum); morphology of the eustachian tube and nasopharynx. | the nasopharynx and eustachian tube (auditory tube) were morphologically examined in the white rhinoceros. the narrow nasopharynx cavity was enlarged dorsoventrally. the most lateral part of both sides sharply rises in a dorsal direction and the volume is estimated at 173 cc. the eustachian tube is 145 mm in length from the auditory bulla to the nasopharynx. the hyaline cartilage is well-developed in the middle region of the eustachian tube wall. the results demonstrated that the white rhinocero ... | 1998 | 9818452 |
| phylogenetic relationships within the genus equus and the evolution of alpha and theta globin genes. | sequences of the alpha1, alpha2 and theta globin genes from six equid species have been determined to investigate relationships within the genus equus. analyses using standard phylogenetic methods, or an approach designed to account for the effects of gene conversion between the alpha genes, gave broadly similar results and show that the horses diverged from the zebra/ass ancestor approximately 2.4 million years ago and that the zebra and ass species arose in a rapid radiation approximately 0.9 ... | 1998 | 9847419 |
| necrosis of the femoral head after kidney transplantation. | we reviewed the medical records of 750 patients (445 men, 305 women), who had received a kidney transplant during the period 1968-1995, for any sign of necrosis of the femoral head. for post-operative immunosuppression, 374 patients had received high-dose corticosteroids (average 12.5 g during the first year post-operatively), while 376 patients had received low-dose corticosteroids (average 6.5 g during the first year post-operatively) and cyclosporin a. survival curves according to kaplan and ... | 1998 | 9850453 |
| seroprevalence of toxoplasma gondii in egyptian donkeys using elisa. | a serological survey of toxoplasma gondii was conducted using elisa on 121 sera from adult donkeys from monofia province, egypt. antibodies to t. gondii were found in 65.6% of 121 donkeys. | 1998 | 9877073 |
| performance and management of draught animals in agriculture in sub-saharan africa: a review. | use of animal power generally enables farmers in sub-saharan africa to increase agricultural production and improve the quality of life. effective use of working animals depends on an understanding of the capabilities of the animals for work, their husbandry requirements and the factors which can influence their performance. these issues are reviewed in this paper in the context of the use of animal power in agriculture in sub-saharan africa. the type of animal used for work determines power ava ... | 1998 | 9881437 |
| novel peptides binding to the fc-portion of immunoglobulins obtained from a combinatorial phage display peptide library. | peptides interacting with the fc portion of human igg (igg fc) were selected from a phage display decapeptide library. the library was selected five times and interacting phage peptides were eluted either with staphylococcal protein a or at low ph. individual peptide phage clones were found to interact more strongly with igg fc than did either the original library or the wild-type phage. increasing concentrations of protein a could competitively reduce the interaction of a peptide phage clone (f ... | 1998 | 9894906 |
| improved protection against venezuelan equine encephalitis by genetic engineering of a recombinant vaccinia virus. | an improved vaccine is needed against venezuelan equine encephalitis (vee) virus because the existing live attenuated vaccine, tc-83, causes a high incidence of adverse effects, and the formalin-inactivated vaccine, c-84, does not protect against airborne infection. a recombinant vaccine had previously been constructed in which the vee structural proteins were expressed by vaccinia virus. although protection against subcutaneous challenge with vee was achieved, the vaccine had limited efficacy a ... | 1998 | 9918402 |
| evaluation of enterolithiasis in equids: 900 cases (1973-1996). | to identify breed, age, sex, physical findings, history, and outcome of treatment in horses and other equids with enterolithiasis. | 1999 | 9926016 |
| four tumor suppressor loci on chromosome 9q in bladder cancer: evidence for two novel candidate regions at 9q22.3 and 9q31. | the most common genetic alteration identified in transitional cell carcinoma (tcc) of the bladder is loss of heterozygosity (loh) on chromosome 9. however, localization of tumor suppressor genes on 9q has been hampered by the low frequency of subchromosomal deletions. we have analysed 139 primary, initial low stage tcc of the bladder using a panel of 28 microsatellite markers spanning chromosome 9 at an average distance of 5 mb, following a primer-extension preamplification (pep) technique. sixt ... | 1999 | 9926930 |
| finite element analysis of static loading in donkey hoof wall. | a finite element model of donkey hoof wall was constructed from measurements taken directly from the hoof capsule of the left forefoot. the model was created with a 2 mm mesh and consisted of 11,608 nodes. a linear elastic analysis was conducted assuming isotropic material properties in response to a 375 newton (n) load, to simulate static loading. the load was applied to the wall via 400 laminae in order to simulate the way in which the pedal bone is suspended within the donkey hoof capsule. di ... | 1998 | 9932100 |
| mutational status of the tumour suppressor gene (p53) in donkey sarcoid tumours. | | 1999 | 10030136 |
| isolation and characterization of the mouse aire gene. | autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (apeced) is a rare autosomal recessive disorder characterized by addison's disease and/or hypoparathyroidism and/or chronic mucocutaneous candidiasis. patients may also have other clinical symptoms both within and outside the endocrine system, mainly as a result of autoimmunity against organ-specific autoantigens. the gene for apeced has recently been identified and termed aire (for autoimmune regulator). apeced is a model of organ-s ... | 1999 | 10049735 |
| comparison of horse chromosome 3 with donkey and human chromosomes by cross-species painting and heterologous fish mapping. | the melanocortin 1 receptor (mc1r), mast/stem cell growth factor receptor (kit), and platelet-derived growth factor receptor alpha (pdgfra) are loci that all belong to equine linkage group 2 (lg2). of these, kit was fluorescent in situ hybridization (fish) mapped to eca3q21 with equine cdna and heterologous porcine bac probes, while mc1r was localized to eca3p12 and pdgfra to eca3q21 with heterologous porcine bac probes. a three-step comparison between eca3 and donkey chromosomes was carried out ... | 1999 | 10051324 |
| kinetic selection of hpv 16 e6/e7-directed antisense nucleic acids: anti-proliferative effects on hpv 16-transformed cells. | the e6/e7-coding sequences of the human papillomavirus type 16 (hpv 16) were probed for kinetic accessibility in vitro by pools of catalytic antisense rna. only long-chain complementary rna and very few antisense sequences with a 3' portion complementary to a 10 nt window within unspliced and spliced e6-coding target sequences showed fast annealing with k(ass) values of up to 10(4) m-1s-1 indicating that the majority of e6/e7 rna sequences are unfavourable targets for antisense inhibitors and ri ... | 1999 | 10075988 |
| covalent structures of bmk as and bmk as-1, two novel bioactive polypeptides purified from chinese scorpion buthus martensi karsch. | complete amino acid sequences of two novel bioactive polypeptides, each containing 66 amino acid residues, bmk as and bmk as-1 purified from the venom of chinese scorpion buthus martensi karsch, have been determined by edman sequencing and mass spectrometry on native proteins, reduced and s-carboxymethylated proteins and their peptides obtained after cleavage with proteolytic enzymes. sequence analysis showed 86.4% structural identity between bmk as and bmk as-1 and also a high sequence similari ... | 1999 | 10080355 |
| prevalence of surra among camels and horses in jordan. | the prevalence of trypanosoma evansi infection among camels and horses in jordan was studied using thick blood smears and inoculation techniques with mice and rats. a total of 437 camels and 83 horses from four climatic zones were surveyed. in addition, 40 donkeys, 32 cattle and 35 goats in contact with infected camels and horses were also tested in the same way. clinical disease was evident in 8.2% of the camels (36 out of 437) and in 9.6% of the horses (8 out of 83). infection was limited only ... | 1999 | 10081806 |
| donkey work. | | 1999 | 10085965 |
| modulation of allospecific ctl responses during pregnancy in equids: an immunological barrier to interspecies matings? | maternal immune recognition of the developing conceptus in equine pregnancy is characterized by the strongest and most consistent alloantibody response described in any species, a response directed almost exclusively against paternal mhc class i ags. this work investigated the cellular immune response to paternal mhc ags in pregnant and nonpregnant horses and donkeys, and in horses carrying interspecies hybrid mule conceptuses. we observed profound decreases in classical, mhc-restricted, ctl act ... | 1999 | 10201987 |
| glucocorticoids mediate the enhanced expression of intestinal type ii arginase and argininosuccinate lyase in postweaning pigs. | arginine metabolism is enhanced in the small intestine of weanling pigs, but the molecular mechanism(s) involved is not known. the objectives of this study were to determine the following: 1) whether glucocorticoids play a role in induction of intestinal arginine metabolic enzymes during weaning; 2) whether the induction of enzyme activities was due to increases in corresponding mrna levels; and 3) the identity of the arginase isoform(s) expressed in the small intestine. jejunum was obtained fro ... | 1999 | 10203553 |
| construction of chromosome-specific paints for meta- and submetacentric autosomes and the sex chromosomes in the horse and their use to detect homologous chromosomal segments in the donkey. | a pilot study comparing horse and donkey karyotypes on a molecular basis was initiated using the chromosomal microdissection approach. all equine meta- and submetacentric chromosomes, viz. eca1 to eca13 and the x and y chromosomes, were microdissected. the dna was pcr amplified, non-radioactively labelled and used as probes on equine metaphase chromosomes to confirm their origin. once tested, the paints were used as probes on donkey metaphase chromosomes to detect homologous chromosomal segments ... | 1999 | 10328622 |
| equine nasal and paranasal sinus tumours: part 2: a contribution of 28 case reports. | the clinical and pathological findings of 28 cases (27 horses, 1 donkey) of equid sinonasal tumours examined at the edinburgh veterinary school are presented and include: seven cases of squamous cell carcinoma (scc); five adenocarcinomas; three undifferentiated carcinomas; two adenomas; five fibro-osseous and bone tumours; and single cases of ameloblastoma, fibroma, fibrosarcoma, undifferentiated sarcoma, melanoma and lymphosarcoma. the median ages of animals affected with epithelial, and fibro- ... | 1999 | 10328839 |
| cloning, sequencing and functional expression of zebra (equus burchelli) lh. | although donkey luteinizing hormone exhibits a very high degree of amino acid sequence identity with horse lh, its fsh activity in non-equine species is tenfold lower. the coding regions of the common zebra (equus burchelli) glycoprotein hormone alpha-subunit and lh beta-subunit transcripts were cloned by reverse transcription-pcr from pituitary gland rna to investigate more precisely the structure-function relationships of this gonadotrophin family. zebra lh was then expressed in cos-7 cells an ... | 1999 | 10341734 |
| steroidogenic factor 1-dna binding: a kinetic analysis using surface plasmon resonance. | basal expression of the glycoprotein hormone alpha-subunit gene in pituitary gonadotrophs is partially dependent on a gonadotroph specific element (gse) which binds the nuclear receptor, steroidogenic factor-1 (sf-1). we have used surface plasmon resonance (spr) to determine the association (kappa ass), dissociation (kappa diss) and affinity (ka) constants of sf-1 binding to immobilized oligonucleotides containing either the gse consensus motif or a gse mutant with a 2 bp substitution in the gse ... | 1999 | 10343283 |
| asthma symptoms: influence of personality versus clinical status. | the hypothesis that symptom-reporting in asthmatics does not necessarily correspond with clinical status, but is related to negative affectivity was investigated. one hundred and sixteen asthmatic patients filled out the asthma symptom checklist (asc), the negative emotionality scale (nem), and the mcmaster-asthma quality-of-life questionnaire (aqlq). the patients were grouped as either hospitalized, outpatient previously hospitalized or outpatient not previously hospitalized for asthma. lung fu ... | 1999 | 10362035 |
| [anion gap in perinatal asphyxia]. | the aim of this study was to assess the usefulness of the anion gap (ag) for the diagnosis of perinatal asphyxia (pa), as well ass to evaluate the relation of ag with umbilical artery cord ph and the outcome of the newborn. we studied 62 infants in whom umbilical artery blood gases were obtained within the first 15 minutes of life. serum sodium, chloride and potassium were simultaneously measured. ag was calculated according to the following formula: (na + k) - (cl + hco3). patients were divided ... | 1999 | 10363420 |
| the gene mutated in adult-onset type ii citrullinaemia encodes a putative mitochondrial carrier protein. | citrullinaemia (ctln) is an autosomal recessive disease caused by deficiency of argininosuccinate synthetase (ass). adult-onset type ii citrullinaemia (ctln2) is characterized by a liver-specific ass deficiency with no abnormalities in hepatic ass mrna or the gene ass (refs 1-17). ctln2 patients (1/100,000 in japan) suffer from a disturbance of consciousness and coma, and most die with cerebral edema within a few years of onset. ctln2 differs from classical citrullinaemia (ctln1, omim 215700) in ... | 1999 | 10369257 |
| factors controlling epidermal growth factor (egf) gene expression in the endometrium of the mare. | previous studies showed a dramatic increase in egf gene expression in the endometrial glands of pregnant mares around day 40 after ovulation. to investigate how the steroid hormones of pregnancy might regulate this expression, in situ hybridization was used to monitor the levels of egf mrna in endometrial biopsies obtained from seasonally anoestrous or ovariectomised mares given exogenous progesterone and oestrogen, alone or in combination, for up to 46 days. biopsies were also taken from mares ... | 1999 | 10369386 |
| phylogenetic relationships of cheju horses to other horse breeds as determined by mtdna d-loop sequence polymorphism. | historical records suggest that horses inhabiting the island of cheju in korea are descendants of mongolian horses introduced in 1276. other studies, however, suggest that horses may have been present on the island prior to the mongolian introduction. to determine the origin of the cheju horses we used a phylogenetic analysis of sequences of the mitochondrial dna (mtdna) d-loop region, including trna pro and parts of trna thr and trna phe sequences (1102-bp excluding the tandem repeat region). m ... | 1999 | 10376300 |
| [the human being from the point of view of animals or, "who knows whether the breath of men goes upwards?" (ecclesiastes 3:21)]. | the question of whether animals have souls has been asked since as early as the old testament. where this is believed to be true, fiction has provided interesting models in literature: the human being as seen by animals has been a popular subject since apuleius' 'asinus aureus' and how man appears from the perspective of a donkey or a beetle, that is to say the perspective from below, becomes controversial. examples may be found in all languages and centuries in jonathan swift, miguel cervantes, ... | 1999 | 10379104 |
| neogenesis vs. apoptosis as main components of pancreatic beta cell ass changes in glucose-infused normal and mildly diabetic adult rats. | we have investigated in adult rats made mildly diabetic by a low dose of streptozotocin (35 mg/kg; stz rats) and in nondiabetic rats (nd rats) the mechanisms leading to adaptive changes in the beta cell mass, during glucose infusion and several days after stopping infusion. as early as 24 h of glucose infusion, the beta cell mass was maximally increased in nd and stz rats. in both groups, this increase was due mainly to a rapid activation of neogenesis of new endocrine cells rather than to an in ... | 1999 | 10385610 |
| detection of equine arteritis virus in semen by reverse transcriptase polymerase chain reaction-elisa. | the reverse transcriptase polymerase chain reaction (rt-pcr) assay was used to detect equine arteritis virus (eav) in the semen of 88 horses and 2 donkeys, with neutralising antibodies against eav, on the basis of the amplification of a 279 bp long fragment located in the viral polymerase gene. the rt-pcr assay revealed the virus at 4 tcid50/ml in cell culture and showed a greater sensitivity (54.4%) than cell culture isolation (33.3%). moreover, the two samples of donkey semen were found positi ... | 1999 | 10391506 |
| limited potential for mosquito transmission of genetically engineered, live-attenuated venezuelan equine encephalitis virus vaccine candidates. | in an attempt to improve the current live-attenuated vaccine (tc-83) for venezuelan equine encephalitis (vee), specific mutations associated with attenuation of vee virus in rodent models were identified. these mutations were inserted into full-length cdna clones of the trinidad donkey strain of vee virus by site-directed mutagenesis, and isogenic virus strains with these mutations were recovered after transfection of baby hamster kidney cells with infectious rna. we evaluated 10 of these strain ... | 1999 | 10403340 |
| real time analysis of interaction between inositol 1,4, 5-trisphosphate receptor type i and its ligand. | inositol 1,4,5-trisphosphate (ip(3)) is an important second messenger that releases intracellular ca(2+) by binding to its specific receptor, inositol 1,4,5-trisphosphate receptor (ip(3)r), in a wide range of cellular processes. we report here large-scale expression and purification of n-terminal 604 amino acids of ip(3)r type 1 (t604) expressed in e. coli, which contains the ligand binding domain. surface plasmon resonance biosensor studies showed that purified t604 could bind to its ligands wi ... | 1999 | 10403801 |
| [abil-ilisu--a "cattle doctor" in babylonia (circa 1739 b.c.)]. | since decades the search for the first "veterinarians" in ancient mesopotamia and egypt known by name is accompanied by speculations. both ur-lugal-edinna from lagash and the sekhmet-priest aha-nakht turned out to be a specific historical fiction. terminologically assured records for the terms "cattle-doctor" and "donkey-doctor" were found not before about 1800 in old babylonian documents and laws. samsu-iluna, successor to hammurapi, ruled from 1749-1712. out of this time there exists a court r ... | 1999 | 10422373 |
| identification of ruminococcus flavefaciens as the predominant cellulolytic bacterial species of the equine cecum. | detection and quantification of cellulolytic bacteria with oligonucleotide probes showed that ruminococcus flavefaciens was the predominant species in the pony and donkey cecum. fibrobacter succinogenes and ruminococcus albus were present at low levels. four isolates, morphologically resembling r. flavefaciens, differed from ruminal strains by their carbohydrate utilization and their end products of cellobiose fermentation. | 1999 | 10427077 |
| an adult-onset case of argininosuccinate synthetase deficiency presenting with atypical citrullinemia. | a 52-year-old heavy drinker presented with repeated episodes of disturbance of consciousness and an increase in serum ammonia level, triggered by excessive alcohol intake. he was diagnosed as having adult-onset citrullinemia with deficiency of hepatic argininosuccinate synthetase (ass) activity. cranial magnetic resonance imaging (mri) showed high-intensity lesions in the central pons and the bilateral middle cerebellar peduncles on t2-weighted images. although almost all cases of adult-onset ci ... | 1999 | 10435367 |
| highly efficient inhibition of human chymase by alpha(2)-macroglobulin. | the inhibition of human chymase by the protease inhibitor alpha(2)-macroglobulin (alpha2m) was investigated. titration of chymase hydrolytic activity with purified alpha2m showed that approximately 1 mol of alpha2m tetramer inhibits 1 mol of chymase. inhibition was associated with cleavage of the alpha2m bait region and formation of a 200-kda covalent complex. nh(2)-terminal sequencing of chymase-treated alpha2m revealed cleavage at bonds phe684-tyr685 and tyr685-glu686 of the bait region. alpha ... | 1999 | 10441378 |
| detection of new dna polymerase genes of known and potentially novel herpesviruses by pcr with degenerate and deoxyinosine-substituted primers. | a consensus primer pcr approach was used to (i) investigate the presence of herpesviruses in wild and zoo equids (zebra, wild ass, tapir) and to (ii) study the genetic relationship of the herpesvirus of pigeons (columbid herpesvirus 1) to other herpesvirus species. the pcr assay, based on degenerate primers targeting highly conserved regions of the dna polymerase gene of herpesviruses, was modified by using a mixture of degenerate and deoxyinosine-substituted primers. the applicability of the mo ... | 1999 | 10456789 |
| induction of argininosuccinate synthetase in rat brain glial cells after striatal microinjection of immunostimulants. | the enzyme argininosuccinate synthetase (ass) initiates the metabolic pathway leading from l-citrulline to l-arginine, the only physiological substrate of all isoforms of nitric oxide synthases. the presence of ass in glial cells in vivo was investigated by immunohistochemical methods in a model of rat brain inflammation. phosphate-buffered saline or a mixture of bacterial lipopolysaccharide and interferon-gamma was injected into the left striatum, and animals were killed 24 hours later. ipsilat ... | 1999 | 10458597 |
| [wild is not really wild: brain weight of wild domestic mammals]. | domestication leads to the reduction of brain weight, decreases reach from 8.1% in laboratory rats up to 33.6% in domesticated pigs. the question is: do brain weights increase by feralization? we compared the brain weights of domesticated mammals (cat, dog, pig, goat, ass) with their feral forms. in none of the cases studied, brain weight is increased in wild domestic mammals. so, feral mammals do not return back to the status of their wild species. | 1999 | 10472721 |
| expression of the genes of arginine-synthesizing enzymes in the rat kidney during development. | the expression of two genes, coding for argininosuccinate synthetase (ass) and argininosuccinate lyase (asl), enzymes which synthesize arginine, was studied by northern analysis in various tissues of fetal rats. the highest expression of both genes was seen in the small intestine, liver and kidney of the fetus. the developmental expression was observed in the kidney where both mrna levels remained low during the fetal period and they increased concomitantly in both kidney and liver throughout th ... | 1999 | 10473900 |
| bovine papillomaviral gene expression in equine sarcoid tumours. | the sarcoid is a benign locally invasive dermal fibroblastic lesion, commonly affecting horses and donkeys. the aetiology of the equine sarcoid is equivocal. bovine papillomaviral (bpv) dna (type 1/2) is frequently demonstrable in equine sarcoid tumour biopsies. however, the exact role of the virus in the disease process and its contribution to the phenotypic differences in sarcoids is not known. it was sought to assess the transcriptional activity of bpv-1 found in sarcoid tissues. of 20 tumour ... | 1999 | 10475087 |
| alternative approaches to control of cyathostomes: an african perspective. | cyathostomes are common nematode parasites of horses, donkeys and mules in developing countries. these equids usually work on a daily basis and this includes transportation of goods, crops and people. stress, associated with work, and limited nutrition often result in a loss of condition, and cyathostomid nematodes may, then, cause clinical disease. because anthelmintics are not always accessible or affordable in many parts of africa, or there may be anthelmintic resistance to current remedies, ... | 1999 | 10485361 |
| erythrocyte associated haemato-biochemical changes in babesia equi infection experimentally produced in donkeys. | equine babesiosis, caused by babesia equi and transmitted by ticks is of major economic importance in india. the adverse effects which b. equi organism and its metabolites inflict on red blood cells have not been reported. erythrocytes were analysed for red cell membrane phospholipids, proteins and haemoglobin (hb) concentration and plasma for malondialdehyde (mda) in b. equi carrier donkeys before splenectomy (< 1% parasitaemia) and after splenectomy at 1-5, 5-15, 15-50 and >50% parasitaemia. b ... | 1999 | 10488734 |
| [argininosuccinate synthetase (ass)]. | | 1999 | 10503476 |
| gos.sp.ass.'98: an assessment for speech disorders associated with cleft palate and/or velopharyngeal dysfunction (revised). | in 1994 the present authors proposed a speech assessment protocol for speech disorders associated with cleft palate and/or velopharyngeal dysfunction known as gos.sp.ass. (great ormond street speech assessment). in a recent survey undertaken to review the different speech assessment protocols used in six cleft palate centres in the uk, gos.sp.ass. was selected from six protocols as the optimal procedure for clinical and research purposes. the process of identifying an optimal procedure involved ... | 1999 | 10505144 |
| age determination in mini-shetland ponies and donkeys. | the accuracy of ageing mini-shetland ponies and donkeys was assessed by correlating the appearance of specific dental features with the known ages of 106 mini-shetland ponies and 63 donkeys. the ages of the animals ranged between 2 days and 26 years. in both species the eruption of the deciduous and permanent incisors occurred later than in horses. on the other hand, the appearance of the dental stars on the permanent incisors of mini-shetlands and donkeys was seen at a younger age than in horse ... | 1999 | 10528536 |
| [acute myocardial infarction in germany between 1996 and 1998: therapy and intrahospital course. results of the myocardial infarction registry (mir) in germany]. | the "myocardial infarction registry" in germany (mir) is a multicenter and prospective registry of consecutively included, unselected patients with acute myocardial infarction. the purpose of mir is to document the actual praxis of decision making and prescribing of an optimized infarction therapy in ami patients. optimized infarction therapy is defined as the combination of reperfusion therapy and ass, betablocker, and ace inhibitor.14,598 patients with acute myocardial infarction were included ... | 1999 | 10552190 |
| specific binding of proinsulin c-peptide to human cell membranes. | recent reports have demonstrated beneficial effects of proinsulin c-peptide in the diabetic state, including improvements of kidney and nerve function. to examine the background to these effects, c-peptide binding to cell membranes has been studied by using fluorescence correlation spectroscopy. measurements of ligand-membrane interactions at single-molecule detection sensitivity in 0.2-fl confocal volume elements show specific binding of fluorescently labeled c-peptide to several human cell typ ... | 1999 | 10557318 |
| kinetics of interaction of trypsin with an anionic inhibitor of trypsin bwi-1a from buckwheat seeds. | the kinetics of binding of bovine trypsin to a proteinaceous inhibitor of trypsin from buckwheat seeds (bwi-1a) has been studied. the association rate constant (k(ass)) was 2.2 x 10(6) m-1 x sec-1 and the dissociation rate constant (k(off)) of the enzyme--inhibitor complex was 3.5 x 10(-3) sec-1; the inhibition constant ki was 1.5 nm. the inhibitor bwi-1a is of the slow, tightly binding type. the mechanism of the inhibition of bovine trypsin by the trypsin inhibitor bwi-1a was studied. the mecha ... | 1999 | 10561554 |
| pharmacokinetics of flunixin meglumine in donkeys, mules, and horses. | to compare serum disposition of flunixin meglumine after i.v. administration of a bolus to horses, donkeys, and mules. | 1999 | 10566824 |
| interactions between neutrophil gelatinase-associated lipocalin and natural lipophilic ligands. | neutrophils are activated by both paracrine molecules, e.g. platelet activating factor (paf) and leukotriene b4 (ltb4), and the bacterial hydrophobic peptide n-formyl-met-leu-phe (fmlp). several mechanisms are involved in regulation of the activation, including receptor endocytosis and ligand breakdown. the interactions between the specific granule protein neutrophil gelatinase-associated lipocalin (ngal), expressed in human neutrophils, and fmlp, paf and ltb4, were investigated by weak affinity ... | 1999 | 10572948 |
| african horse sickness in portugal: a successful eradication programme. | african horse sickness (ahs) was diagnosed for the first time in southern portugal in autumn 1989, following outbreaks in spain. ahs virus presence was confirmed by virus isolation and serotyping. an eradication campaign with four sanitary zones was set up by central veterinary services in close collaboration with private organizations. vaccination began on 6 october. in february 1990, vaccination was extended to all portuguese equines (170000 animals). there were 137 outbreaks on 104 farms: 206 ... | 1999 | 10579455 |
| detection and effects on platelet function of anti-platelet antibody in mule foals with experimentally induced neonatal alloimmune thrombocytopenia. | horse mares carrying mule foals were immunized during the last trimester of pregnancy with whole acid-citrate-dextrose-anticoagulated donkey blood to experimentally induce neonatal alloimmune thrombocytopenia. thrombocytopenia occurred in the neonatal mule foals born to immunized horse mares within 24 hours after ingestion of their dams' colostrum. mule foals born to mares not immunized with donkey blood did not develop thrombocytopenia. these findings suggest that antibodies may have been direc ... | 1999 | 10587252 |
| sensory nerve endings in the hard palate and papilla incisiva of the goat. | the sensory innervation of the papilla incisiva in the hard palate of the domestic goat was studied with light and electron microscopy, supplemented by electrophysiological studies of free nerve endings. the goat lacks incisor teeth. grass and leaves are not bitten, but pulled off by pressing them between the tongue and papilla incisiva. thus, the masticatory mucosa is subject to particularly heavy mechanical loads requiring functional specialization of the horny epithelium in the form of thicke ... | 1999 | 10591896 |
| an apparently gluten-induced photosensitivity in horses. | primary photosensitization was observed in 3 appaloosa mares. the skin lesions were diffuse erythema followed by edema and subsequently weeping and finally dry gangrene and ulceration. the severe photosensitivity dermatitis was apparently induced by gluten ingestion. resolution of lesions occurred after withdrawal of the suspected dairy concentrate feed and prevention of exposure to sunlight. neither the ponies nor donkey, which were not fed with the suspected concentrate, exhibited similar skin ... | 1999 | 10592948 |
| genomic structure of the adult-onset type ii citrullinemia gene, slc25a13, and cloning and expression of its mouse homologue. | citrullinemia is an autosomal recessive disease characterized by an argininosuccinate synthetase (ass) deficiency. adult-onset type ii citrullinemia (ctln2) is a form of the disease that is defined by a quantitative decrease in ass protein, but with normal kinetic properties. the gene causing ctln2 (slc25a13) was identified by positional cloning (from 7q21.3) and found to encode a putative calcium-dependent mitochondrial carrier protein. to facilitate mutation analysis, here we describe the intr ... | 1999 | 10610724 |
| kinetic measurements of binding of galectin 3 to a laminin substratum. | galectin 3, a beta-galactoside binding protein, contains a c-terminal carbohydrate recognition domain (crd) and an n-terminal segment including multiple repeats of a proline/tyrosine/glycine-rich motif. previous work has shown that galectin 3 but not the isolated crd binds to laminin, a multivalent ligand, with positive cooperativety indicating the formation of multiple interactions although the lectin in solution is monomeric. using surface plasmon resonance, we find that hamster galectin 3 at ... | 1999 | 10619709 |
| characterization of heparin binding of human extracellular superoxide dismutase. | the c-terminal domain of human extracellular superoxide dismutase (hec-sod) plays a crucial role in the protein's interaction with heparin. here we investigated this interaction in more detail by comparing the heparin-binding characteristics of two variants of hec-sod: the two fusion proteins containing the hec-sod c-terminal domain and a synthetic peptide homologous to the c-terminal. the interaction studies were performed using a surface plasmon resonance based technique on a biacore system. i ... | 2000 | 10625498 |
| [thrombo ass in the prophylaxis of vascular disorders in antiphospholipid syndrome]. | the aim of the study was to try thrombo ass in combined therapy of patients with antiphospholipid syndrome (aps), to evaluate its efficacy in prevention of recurrent vascular defects. thrombo ass tablets (50-100 mg) were included in combined treatment of 45 patients with aps (6 males and 39 females, mean age 36.1 +/- 11.7 years, mean aps duration 10.2 +/- 9.0 years) and 8 patients with sle (1 male and 7 females) matched for age. antiphospholipin antibodies and clinical status were assessed befor ... | 1999 | 10635651 |
| in vitro and in vivo evaluation of hypericin for photodynamic therapy of equine sarcoids. | the therapeutic potential of the photodynamic compound, hypericin, in the treatment of equine sarcoids was evaluated. the in vitro cytotoxicity was assessed using three equine cell lines and the observed phototoxic effect was comparable to that on different highly sensitive human cell lines and significantly influenced by the energy density used although independent of the cell type. the in vivo antitumoural action of photodynamic therapy using hypericin was evaluated on three equine sarcoids in ... | 2000 | 10640414 |
| cell proliferation patterns during development of the equine placenta. | placentation involves considerable growth and reorganization of both maternal and fetal tissues. in this investigation, immunohistochemical localization of the proliferation marker ki-67 antigen was used to monitor cell division during placentation in mares. endometrial biopsies were obtained from eight mares between day 14 and day 26 of pregnancy and from eight anoestrous mares that had been treated with various combinations of progesterone and oestrogen. samples of endometrium and fetal membra ... | 1999 | 10645255 |