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structure and expression of ascaris suum collagen genes: a comparison with caenorhabditis elegans.we are interested in the structure and organization of collagen and collagen genes in the parasitic nematode ascaris suum and in the control of collagen gene expression in ascaris. in this nematode, as in all others studied, collagens constitute the major component of the extracellular cuticle and ultimately we would like to correlate the expression pattern of ascaris cuticular collagen genes with the structure of the nematode cuticle. we would also like to see if there is any correlation betwee ...19901978528
the cuticular biology in developmental stages of ascaris suum.the cuticles from distinct developmental stages of ascaris suum were isolated by a combination of mechanical disruption and detergent treatment of larvae or by surgical removal of cuticle from adults. proteins from the isolated cuticles were solubilized with sds and 2-mercaptoethanol (2me) and analyzed by page. cuticular proteins from the third and fourth larval stages (l3 and l4) were comparable to adult, but differences in the number of bands were observed. the soluble proteins from the adult, ...19901978529
analysis of the cuticular collagens of ascaris suum.the nematode cuticle is an extracellular structure composed mainly of collagens, with an insoluble epicuticle on the surface. the extracted collagens from adult ascaris suum can be separated by sds-page into three major groups of polypeptides with apparent molecular masses of 34, 60-70 and 120-140 kda. densitometric evaluation of the polypeptide bands indicated that the three groups are present in the ratio of 1:2:6. rotary shadowing of reduced, extracted molecules showed fibers 45 nm in length. ...19901978530
antibodies against the cuticlin of ascaris suum cross-react with epicuticular structures of filarial parasites.the insoluble cuticlin from the cortical zone of the cuticle of adult ascaris suum was purified and used to raise antibodies in c57/bl mice. the specificity of the antibodies for the external cortical layer was shown in the indirect immunofluorescence antibody test and by immunoelectronmicroscopy. a very high specificity for the external cortical layer was found. some cross-reactions with cuticular collagens occurred, and increased after booster immunizations. the anti-cuticlin antibody cross-re ...19901978533
the in vivo and in vitro activity of ahr-13268d, a new antiallergic/antihistaminic agent.ahr-13268d (4-[3-[4-[bis(4-fluorophenyl)hydroxymethyl]-1- piperidinyl]propoxy]benzoic acid, sodium salt) is a potent, long-acting water soluble, antiallergic and antihistaminic agent. ahr-13268d protects sensitive guinea pigs from collapse induced by aerosolized antigen; 1, 5, and 24 h ed50s in the test were 0.27, 0.25, 0.93 mg/kg, po, respectively. ahr-13268d was also active when given as an aerosol, the 1 h ed50 = 0.29%. in the rat passivefoot anaphylaxis test. ahr-13268d was slightly more act ...19901982201
propionyl-coa condensing enzyme from ascaris muscle mitochondria. i. isolation and characterization of multiple forms.the condensation of two propionyl-coa units or a propionyl-coa with acetyl-coa is required for the synthesis of 2-methylvalerate or 2-methylbutyrate, respectively, two of the major fermentation products of ascaris anaerobic muscle metabolism. an enzyme that preferentially catalyzes the condensation of propionyl-coa rather than acetyl-coa has been purified from the mitochondria of the parasitic intestinal nematode ascaris lumbricoides var. suum. the purified enzyme is over 10 times more active wi ...19911990976
kinetic mechanism of nad:malic enzyme from ascaris suum in the direction of reductive carboxylation.initial velocity studies in the absence and presence of product and dead-end inhibitors suggest a steady-state random mechanism for malic enzyme in the direction of reductive carboxylation of pyruvate. for this quadreactant enzymatic reaction (mn2+ is a pseudoreactant), initial velocity patterns were obtained under conditions in which two substrates were maintained at saturating concentrations while one reactant was varied at several fixed concentrations of the other. data from the resulting rec ...19911993653
etiology of childhood diarrhea in beijing, china.to determine the role of recently recognized enteropathogens in childhood diarrhea in china, 221 children with diarrhea and 108 controls seen at the beijing children's hospital were studied during april and may 1989. stools were examined for ova, parasites, and rotavirus, cultured for bacterial pathogens, and probed for enterotoxigenic escherichia coli (etec), enteroinvasive e. coli (eiec), enterohemorrhagic e. coli (ehec), and enteropathogenic adherence factor-positive (eaf+) e. coli. pathogens ...19911993771
antigen-induced phospholipase d activation in rat mast cells is independent of protein kinase c.in the present study, we investigated the involvement of protein kinase c (pkc) in antigen (ag, dnp-ascaris suum)-induced phospholipase d (pld) activation of rat peritoneal mast cells. phorbor myristate acetate (pma) as well as ag activated pld as inferred by phosphatidylethanol (pet) production. pkc inhibitors, staurosporine and h-7, however, failed to suppress pma-stimulated pld activation, suggesting that pld activation by pma is independent of pkc. by contrast, ag-stimulated pld activity was ...19911998501
eliminated chromatin of ascaris contains a gene that encodes a putative ribosomal protein.chromatin diminution in the nematodes parascaris equorum and ascaris lumbricoides leads to the formation of somatic cells that contain less dna than the germ-line cells. we present molecular evidence for the coding potential of germ-line-specific dna. we report on a cdna clone that codes for a putative ribosomal protein (alep-1, for a. lumbricoides eliminated protein 1). that the corresponding gene is located in the eliminated portion of the genome indicates a difference in germ-line and somatic ...19912000367
the roundworm, ascaris lumbricoides.ascaris lumbricoides is a significant health problem. one billion people worldwide are infected. most frequently it is seen in malnourished people residing in developing countries. areas with modern water and waste treatment have a low incidence. the major serious sequelae associated with the parasite is intestinal obstruction, which occurs at a rate of 2 per 1000 people infected. intestinal obstruction is fatal in 6 per 100,000 children. an estimated 20,000 people die of this infection annually ...19912011640
sonographic appraisal of endobiliary ascaris with calculi. 19912012059
effects of single and multiple inhalations of antigen on airway responsiveness in monkeys.airway hyperresponsiveness is an important and characteristic feature of asthma. in monkeys, we have reported that antigen (ag) inhalation induces a prolonged airway eosinophilia, that chronic airway eosinophilia is associated with marked airway hyperresponsiveness, and that chronic ag inhalations induce airway eosinophilia and hyperresponsiveness. in this study we have determined the effects of acute ag inhalation(s) on airway responsiveness to obtain a protocol for the study of the mechanisms ...19912013678
epidemiological study of parasitic infestations in lower socio-economic group in chandigarh (north india).when stool samples from 970 subjects belonging to lower socio-economic groups were examined for parasites, a total of 121 subjects (12.5%) i.e., 57 (12.1%) males and 64 (12.9%) females showed positive results. the overall prevalence of parasitic infestation did not correlate with sex, caste or religion and living conditions. however, the prevalence was higher in hospital employees residing in well sanitated area. giardia lamblia (69.5%), entamoeba, histolytica (15.7%), hymenolepis nana (12.4%), ...19912022402
separation of late bronchial responses from airway hyperresponsiveness in allergic sheep.allergic sheep with antigen-induced early and late responses were used to determine whether airway hyperresponsiveness (ahr) to carbachol is present during the late response and whether blocking the late response with the leukotriene d4 (ltd4) antagonist mk-571 also blocks this ahr. to do this, we first showed that mk-571 blocked the antigen-induced late response, and then, in a separate study, we determined the effect of mk-571 treatment on airway responsiveness 6 h after antigen challenge (at ...19912022553
surgical complications of ascariasis.over the past 25 years (1963-1988), a total of 311 children under 12 years of age were admitted to the pediatric surgical service of the san vicente de paúl university hospital, medellín, colombia, with complications resulting from infection with entamoeba histolytica or ascaris lumbricoides. in this group, the abdominal complications produced by ascariasis numbered 145, and included intestinal obstruction (n = 107), perforation of the appendix (n = 10), and migration of the parasite to the bili ...19912031358
airways vasodilatation in the immediate allergic reaction. involvement of inflammatory mediators and sensory nerves.1. systemic capsaicin treatment of the pig depletes the content of sensory neuropeptides (cgrp and tachykinins) in the airways mucosa and skin, without affecting sympathetic and parasympathetic nerves containing npy and vip, or the presence and appearance of inflammatory cells including mast cells. acute capsaicin exposure caused release of sensory neuropeptides and catecholamines, and marked vasodilation in the airways and skin, without signs of plasma protein extravasation or bronchoconstricti ...19912035338
mhc class ii (i-a) region control of the ige antibody repertoire to the aba-1 allergen of the nematode ascaris.aba-1 is an approximately 14,000 molecular weight (mw) allergen which is among the most abundant proteins synthesized by the nematode parasite ascaris. igg and ige responses to it are major histocompatibility complex (mhc)-restricted in rodents and have only been found to occur in rats of the rt1u haplotype and mice of the h-2s haplotype. humans infected with the parasite vary substantially in their immune response to the allergen, but the genetic basis for this unknown. h-2 recombinant mice wer ...19912037317
intestinal parasites in a rural community in kenya: cross-sectional surveys with emphasis on prevalence, incidence, duration of infection, and polyparasitism.a cross-sectional survey of intestinal parasitic infection in a rural community, nderu, in kiambu district, kenya, was carried out in 1985 by examining 1129 individuals from 203 households (about 25% of the total population). this was followed by 3 more cross-sectional surveys, in january, may and october 1986, of 56 families comprising 461 individuals, who had also participated in the first survey. in the first survey, 81.4% of the sample was positive for at least one intestinal parasite and 78 ...19912040230
the highly variable pentameric repeats of the at-rich germline limited dna in parascaris univalens are the telomeric repeats of somatic chromosomes.we have characterized the organization of the germline limited dna of p. univalens by means of sequence analysis. the repeat unit of this satellite dna is the pentanucleotide 5'ttgca, although there is a high degree of sequence variation. repeat variants are not arranged in tandem but in a disperse, nonrandom manner. in the somatic genome which arises from the germline genome through extensive genomic rearrangement early in development, copies of these pentamers represent the telomeric repeats, ...19912041744
desensitization by glycyrrhetinic acid of other stimulatory substance-induced increases in intracellular calcium in a variety of cell types.the effects of glycyrrhizin and its aglycone, glycyrrhetinic acid, on the cytoplasmic free calcium concentration ([ca2+]i) in mastocytoma p-815 cells, dnp-ascaris (antigen) sensitized mast cells, hepatocytes, fibroblasts and endothelial cells were investigated. in these cell types, glycyrrhetinic acid in the concentration range of 20 to 100 microm caused an increase in [ca2+]i and inhibited calcium increases induced by an antigen (mast cells), atp, phenylephrine (hepatocytes) and thrombin (fibro ...19912043161
multiple isotope effects with alternative dinucleotide substrates as a probe of the malic enzyme reaction.deuterium isotope effects and 13c isotope effects with deuterium- and protium-labeled malate have been obtained for both nad- and nadp-malic enzymes by using a variety of alternative dinucleotide substrates. with nicotinamide-containing dinucleotides as the oxidizing substrate, the 13c effect decreases when deuterated malate is the substrate compared to the value obtained with protium-labeled malate. these data are consistent with a stepwise chemical mechanism in which hydride transfer precedes ...19912043615
modification of a thiol at the active site of the ascaris suum nad-malic enzyme results in changes in the rate-determining steps for oxidative decarboxylation of l-malate.a thiol group at the malate-binding site of the nad-malic enzyme from ascaris suum has been modified to thiocyanate. the modified enzyme generally exhibits slight increases in knad and ki metal and decreases in vmax as the metal size increases from mg2+ to mn2+ to cd2+, indicative of crowding in the site. the kmalate value increases 10- to 30-fold, suggesting that malate does not bind optimally to the modified enzyme. deuterium isotope effects on v and v/kmalate increase with all three metal ion ...19912043616
biliary ascariasis.this paper describes the management of 12 cases of biliary ascariasis at the chittagong medical college & hospital, chittagong, bangladesh. the diagnosis may be done clinically or on the basis of mere suspicion if sophisticated investigations like ultrasonography scanning etc. are not available. laparotomy and exploration of the bile duct is essential as the condition does not improve by conservative treatment. the ascaris lumbricoides can be removed by strong suction from the bile duct. retaine ...19912045248
mass treatment of intestinal parasites among ethiopian immigrants.intestinal parasites are common among the ethiopian immigrants to israel and mass treatment is necessary to prevent local transmission. for this purpose, stool samples obtained from the immigrants in absorption centers were examined. of 5,412 samples obtained, 4,399 (81.3%) were positive: 2,644 (54.2%) for necator americanus, 2,273 (46.6%) for schistosoma mansoni, 990 (20.3%) for ascaris lumbricoides, 1,040 (21.3%) for hymenolepsis nana, 940 (19.2%) for trichuris trichiura, 219 (4.5%) for strong ...19912050509
characterization of cdna clones for the beta subunit of pyruvate dehydrogenase from ascaris suum.the pyruvate dehydrogenase complex occupies a unique position in the anaerobic mitochondrial metabolism of the parasitic nematode ascaris suum. this paper describes cdna clones for the beta subunit of the pyruvate dehydrogenase component of this complex. a cdna library has been constructed in lambda gt11 from poly(a)+ rna isolated from adult ascarid body wall muscle. the library was screened with antiserum prepared against the beta subunit of pyruvate dehydrogenase. full-length clones of 1.2 kb ...19912052042
in vivo studies of factors influencing mast cell numbers in rat skin.in order to examine the biological relevance of known in vitro stimuli for mast cell growth, the following substances were injected at two day intervals into the skin of wistar rats: ascaris ag, epidermal growth factor, basic fibroblast growth factor, fibronectin, phytohemagglutinin-stimulated spleen cell supernatants, l-cell fibroblast supernatants and horse serum alone or in combination with l-cell supernatants. in some experiments, rats were also injected with fresh or cultured peritoneal cel ...19912054335
biliary ascariasis--a worm in the duct.migration of ascaris lumbricoides into the biliary tree may cause biliary obstruction, cholangitis or pancreatitis. although common in endemic areas, none of these complications have previously been reported in australia. we report here a case of obstruction of the common bile duct by an ascarid.19912056950
neuropeptides in the nematode ascaris suum. 19912057216
ascariasis and trichuriasis in cameroon.a national survey of ascaris lumbricoides and trichuris trichuria was carried out in cameroon on more than 22,000 children from a random sample of 512 schools. prevalence rates of both a. lumbricoides and t. trichuria infection were very low in the tropical zone (below 5%). they increased markedly in the equatorial zone, guinea-type climate, to 60-85% for a. lumbricoides and 85-95% for t. trichuria. in the equatorial zone with cameroon-type climate, prevalences were slightly lower: 50-70% for a. ...19912068772
the rate of ingestion of ascaris lumbricoides and trichuris trichiura eggs in soil and its relationship to infection in two children's homes in jamaica.the rate of exposure to geohelminth infection of children living in 2 institutions in jamaica, west indies, was estimated from the product of their rates of ingestion of soil and the density of parasite eggs in the environment. the estimated mean rate of egg ingestion was 9-20 ascaris lumbricoides eggs and 6-60 trichuris trichiura eggs per year, although the distribution was highly overdispersed so that some individuals had an estimated annual exposure of several hundred eggs. the estimated indi ...19912068773
[evaluation of the prevalence of the main enteropathogenic agents in the west imerina region: manarintsoa].for a 288 children's population aged from 5 to 14 years old and being school attendents in the village of manarintsoa, we have systematically investigated 60% of them in may 1988 and january 1989. bacterial, parasitical and viral study of these stools showed off an important number of a. dispar and e. coli strains which have special phenotypes characters. the prevalence of campylobacter and ascaris lumbricoïdes is higher in this rural zone than in tananarive. two poliovirus of type 2 have been i ...19902078083
[intestinal parasites and lactose malabsorption].breath-test was performed for diagnosis of lactose malabsorption in 50 gabonese children of normal nutritional status, aged 5 to 15 years, with parasites in stools, but without diarrhoea or digestive symptoms. control group was unparasitized and consisted of 17 children and 18 young adults living in the same area. parasites discovered by stool examination were ascaris lumbricoides in 76% of parasitized children, trichuris trichiura in 58%, giardia in 24%, entamoeba histolytica in 20%, schistosom ...19902078108
oxidation-reduction potentials of cytochromes in ascaris muscle mitochondria: high-redox-potential cytochrome b558 in complex ii (succinate-ubiquinone reductase).oxidation-reduction midpoint potentials (ems) were determined at ph 7.0 for cytochromes in the anaerobic respiratory chain of ascaris mitochondria by redox titration techniques. cytochrome b558, which is associated with complex ii that functions as fumarate reductase in the terminal step of the respiratory chain, was shown to have an em of -34 mv in the isolated complex ii and -54 mv in mitochondria. these values are much higher than the value of ascaris cytochrome b558. in contrast, ems of cyto ...19902080921
prevalence of salmonella, shigella and intestinal parasites in food handlers in irbid, jordan.during a one-year period, 283 food handlers in irbid, jordan were investigated for the presence of potential enteropathogens in their stools. the prevalence rate of enteropathogens among non-jordanian food handlers (48.0%) was significantly higher (p less than 0.05) than that of the jordanian group (12.3%). the isolation rates of salmonella and shigella were 6% and 1.4% respectively. multi-drug resistance was frequent among isolates of salmonella group b and shigella spp. intestinal parasites de ...19902081882
the immunotoxicity of triphenyltin chloride in mice.the immunotoxicity of triphenyltin chloride (tptc) in mice was investigated. 1) when tptc was injected intraperitoneally (i.p.) into mice at doses between 1 and 10 mg/kg for 14 d, a reduction in the weights of thymus and spleen was noticed, however, the body weight was not changed significantly. 2) the production of hemolytic plaque forming cells in the spleen of mice immunized with sheep red blood cells (srbc) was inhibited by the administration of 10 mg/kg of tptc. 3) the i.p. injection of tpt ...19902084225
[clinical analysis of 90 cases of eosinophilia and pulmonary infiltrates with peripheral eosinophilia].90 patients diagnosed as eosinophilia and pulmonary infiltrates with peripheral eosinophilia in pumc hospital from 1957 to 1986 were reported. the cause of the eosinophilia was unknown in 42% of the patients. among these patients, hypereosinophilic syndromes (hes) were suspected in 11 patients. the most common presenting symptoms of hes were fever, skin lesions gastrointestinal symptoms and hepatosplenomegaly, the mean peripheral blood eosinophil count was as high as 25.6 x 10(9) cells/l. biopsi ...19902086006
[the study of ascaris lumbricoides in the laboratory].with the purpose of standardising techniques for the laboratory study of ascaris lumbricoides, faeces were collected from children parasitised by a. lumbricoides, during a 24 hour period. the fecal samples were sieved and resuspended several times in water. the sediment containing the eggs was cultivated in h2so4 0.1n in tissue culture flasks, at 28 degrees c. the culture of embryonated eggs was determined every ten days starting from the 20th day of culture achieving around 98.0% embryogeny on ...19902089481
[levamisole, mebendazole, thiabendazole. their effectiveness as helminthicides in african patients recently arrived in cuba. from 1987 to 1988].ninety one passengers coming from africa, carriers of geohelminthes, were selected and treated with helminthicides of large use in our country (levamisole, mebendazole, thiabendazole). an excellent effective response was obtained for ascaris lumbricoides (96.5%), and a good effective response was obtained for the rest of the parasites: trichuri trichiura (88.5%), strongyloides stercoralis (87.5%), and necator americanus (78.9), according to data of therapeutical response efficiency of the who.19902089506
[prevalence of entamoeba histolytica and other intestinal parasites in a neighborhood of the mara municipality, zulia state, venezuela].a parasitologic survey of a low socioeconomic level community from mara county, zulia state, venezuela was done. a single stool specimen per person from 327 individuals was obtained and examined by hematoxylin stained fecal smears and formol-ether concentrates. the overall parasitic infection rate was 92%. multiple infections were present in 89% of the population. the amebic prevalence rate (apr) was 41.5%. entamoeba histolytica was found in 9.2% of the individuals, most of them being asymptomat ...19902090263
quantitative assessment of contamination of soil by the eggs of ascaris lumbricoides and trichuris trichiura.this study used a method of retrieving eggs from soil to examine the spatial and temporal dynamics of soil contamination with geohelminth eggs. the level of soil contamination in two children's homes in jamaica was determined before and after further soil contamination was prevented by chemotherapy. the home which had higher human infection levels also had a higher prevalence and density of eggs in soil. the spatial distribution of the eggs in soil was overdispersed in the home with higher level ...19902091353
a survey of gastrointestinal parasites in pigs of the plateau and rivers states, nigeria.faecal samples were collected from a total of 1,000 pigs from the port harcourt and jos areas of the rivers and plateau states, respectively, between january 1987 and march 1988. in the jos area the parasite incidence was: ascaris suum 53.1%, trichuris suis 8.5%, hyostrongylus rubidus 13.1%, metastrongylus salmi 3.7%, strongyloides ransomi 87.7%, oesophagostomum dentatum 35.1% and eimeria spp. 2.4% while in port harcourt the incidence rate was ascaris suum 10.4%, trichuris suis 47.2%, oesophagos ...19902092354
[studies of serum lipids in guinea pigs with larval ascariasis].the experiments were carried out on a group of 90 males of guinea pigs of 300 +/- 50 g in weight. the animals were divided into 6 groups equal in numbers. 5 of them were infected per os with ca 5000 invasive eggs of ascaris suum. the blood of both infected and control guinea pigs was controlled on 1, 2, 4, 7, and 20th day after infection. the level of total lipids (according to swahn method), wkt (dole titrimetric method), total cholesterol and hdl cholesterol (błaszczyszyn method) and triglycer ...19902092676
the "crowding effect" phenomenon in ascaris lumbricoides.ascaris lumbricoides worm populations were studied from 150 filipino children (0-14 years) dewormed with pyrantel pamoate (combantrin) or oxantel-pyrantel pamoate (quantrel). populations were classified according to intensity of infection: very light, light, moderate or heavy. a total of 2072 adult worms were sexed (939 males and 1133 females) and weighed. descriptive statistics on the weights obtained from each sex were derived and one-way anova was performed to compare the mean weights among t ...19902098920
[a survey on the epidemiological factors of clonorchiasis in the pohang industrial belt along the hyungsan river, kyongsangbuk-do].the employees at the pohang industrial area, where clonorchis sinensis has been known to be endemic along the hyungsan river, were examined parasitologically for clonorchiasis and a part of the infected cases were surveyed with a questionnaire to outline the recent infection status of c. sinensis and epidemiological parameters in the area. total of 3,180 cases were tested by intradermal inoculation of c. sinensis antigen (green cross co., korea), and 834 (26.2%) were found positive. out of the p ...19902099175
embryonation and infectivity of ascaris suum eggs. a comparison of eggs collected from worm uteri with eggs isolated from pig faeces.ascaris suum eggs were collected from pig faeces or dissected from worms obtained from the same pigs. eggs from the two sources were allowed to embryonate in 0.1 n h2so4, in 1% buffered formalin or in tap water. the embryonation of the sulphuric acid and water cultures occurred at the same speed, while the formalin cultures developed slightly more slowly. by experimental inoculation of helminth-free pigs and subsequent counting of white spots in the livers and larvae in the lungs day 7 p.i., the ...19902099616
ascaris suum: influence of egg density on in vitro development from embryonated egg to infective stage. 19902099626
further characterization of ascaris suum component(s) with suppressive activity on the ige antibody response.in order to characterize the component(s) of ascaris suum responsible for damping of the ige antibody production we demonstrated that the extract incubated in sodium acetate buffer, ph 4.5, maintained its suppressive effect and the same protein banding pattern by sds-page. elimination of the lipoprotein components of the extract also left its damping properties unchanged. sds-page of the lipoprotein-free extract revealed practically the same pattern as shown by the whole extract, except for the ...19902101078
a profile of diarrhoea in an urban slum area.researchers followed 90 households (445 people) in sunderpur slum in varanasi in upper pradesh, india for 1 year and collected stool samples when people were ill with diarrhea to determine diarrhea incidence and causes of diarrheal disease. the water supply consisted of a well, public tap, or house tap with 30 households in each group. they noted 106 diarrheal episodes for an incidence of around 23%. incidence decreased significantly with age (p.001). for example, it was 62.9% for children 5 ...19902101391
[obstructive jaundice caused by ascaris lumbricoides].in middle europe biliary complications caused by ascaris lumbricoides are very rare. we report on endoscopic removal of an ascaris out of the common bile duct in a 81-years-old woman.19902102035
helminths and protozoa as an incidental finding in cytology specimens.this is a description of some helminths and protozoa found incidentally in routine cytology specimens submitted to this laboratory. eight different organisms are described together with the case history for each patient. all the specimens were stained with papanicolaou technique.19902102355
divalent hapten-induced intestinal anaphylaxis in the mouse enhances macromolecular uptake from the stomach.the capacity of the stomach to participate in anaphylaxis induced by the hapten n,n'-di-2,4,dinitrophenyllysine (di-dnp-lysine) was examined in bdf1 female mice immunized with dinitrophenylated ascaris suum extract. immunized animals underwent laparotomy and nontraumatic pyloric occlusion using a microvascular clamp. following wound closure, animals were gavage-fed ovalbumin together with di-dnp-lysine. other mice were subjected to systemic anaphylaxis by intravenous injection of di-dnp-lysine a ...19902107115
mechanisms of decrease in cytoplasmic motility of alveolar macrophages during immediate asthmatic response in dogs.to study the cytoplasmic motility (cm) of alveolar macrophages (am) during immediate asthmatic response, we measured remanent filed strength (rfs) in dogs in vivo after ascaris suum-antigen (aa) inhalation. four days after instillation of ferrimagnetic particles (fe3o4, 3 mg/kg) into the right lower lobe bronchus, rfs was measured at the body surface immediately after magnetization of the fe3o4 particles by an externally applied magnetic field. rfs decreased with time because of particle rotatio ...19902110421
a myofibrillar protein of insect muscle related to vertebrate titin connects z band and a band: purification and molecular characterization of invertebrate mini-titin.we show that myofibrils of insect flight and leg muscle contain a doublet of polypeptides with apparent molecular weights of 700k (k = 10(3) mr) (hmp i) and 600k (hmp ii), respectively. in locusta migratoria high ionic strength extraction solubilizes only hmp ii, which is readily purified in native form. it probably reflects a proteolytic derivative of the non-extractable hmp i. on the basis of its viscosity radius and sedimentation coefficient, hmp ii has a molecular weight of 600k and seems to ...19902117014
hookworm infection and protein-energy malnutrition: transverse evidence from two malaysian ecological groups.anthropometric and parasitological data from cross-sectional studies of two groups of primary school children (group i of indian origin, 325 boys and 259 girls, age = 7 years; group ii of malay origin, 284 boys and 335 girls, age = 7-9 years) from two different ecological settings in peninsular malaysia were examined for epidemiological evidence of an association between hookworm infection and protein-energy malnutrition. in both ecological groups, significant weight, height and haemoglobin defi ...19902124397
the prevalence of parasitism in preschool americans in the philippines.one hundred and two american children living at clark air base in the philippines had stools tested for parasites. seven (6.9%) were positive, isolating ascaris (3), giardia (3), and tricuris (2), but no correlation was found in sex, race, or place of residence. fourteen children whose parents felt they had symptoms of parasites tested negative. when stationed overseas in a parasite-endemic area, parents worry about their children acquiring parasites, but we were able to confirm a low prevalence ...19902125337
use of human colostrum in the management of chronic infantile diarrhoea due to enteropathogenic e. coli infection with associated intestinal parasite infestations and undernutrition.this study reports the management of infants with chronic diarrhoea by colostrum feeding. eight children with chronic diarrhoea, ranging from 9 months to 3 years of age and all from low socio-economic families, formed the basis of this study. they were undernourished and marasmic. stool examination showed enteropathogenic e. coli in all eight cases, ascaris lambricoidis in four, and giardia lamblia in one. patients with chronic diarrhoea, in whom no cause was found were excluded from this study. ...19902126564
water demand and schistosomiasis among the gumau people of bauchi state, nigeria.a helminthological study of stool and urine samples from 1037 people from 4 rural communities in gumau district of bauchi state, nigeria, was carried out using formol-ether concentration and direct centrifugation methods respectively. 39.0% of persons examined were infected. ova of ascaris lumbricoides, schistosoma haematobium and s. mansoni were the most common. trichuris trichiura, hookworm, tapeworm and strongyloides stercoralis were also encountered. 17.9% and 10.8% of examined persons had s ...19902128667
are there interactions between schistosomes and intestinal nematodes?a survey for intestinal parasites was carried out in a homogenous rice cultivation area, in which people had equal opportunities of acquiring the local endemic helminthiases, including schistosomiasis mansoni. the numbers of schistosoma mansoni eggs excreted in faeces were counted. infections with s. mansoni, ascaris lumbricoides, ancylostomes and trichuris trichiura were not randomly distributed, but were correlated, depending on the species of worms present. the s. mansoni egg counts were posi ...19902128670
strongyloides cf. fuelleborni and other intestinal helminths in papua new guinea: distribution according to environmental factors.age graded prevalence rates for strongyloides cf. fuelleborni, hookworm, ascaris and trichuris from various regions of papua new guinea were standardised using the standardised mortality technique. using regression analyses on the resultant standardised prevalence ratios (sprs) of the four intestinal helminths and rainfall, altitude, slope and relief values, and population density for the various regions no environmental preference was shown which would account for their distribution. however, a ...19902132436
[treatment of ascaridiasis with garlic (allium sativum)].in brazil, there has been growing interest on alternative therapeutic approaches, as shown by asi increase of homeopathic prescriptions, and use of acupuncture and of the so called natural products. this study evaluated the usefulness of garlic (allium sativum) for the treatment of ascaridiasis, trying to confirm the popular belief on this matter. eight grams daily, as an infusion were used during five days, in children known to be infected with ascaris lumbricoides. little efficacy was observed ...19902133175
epidemiology and control of ascariasis in korea.in view of the epidemiology and control strategy, ascariasis was reviewed with special reference to the data obtained for the past years in korea. the range of length and prepatent period of a. lumbricoides to become fertile were 12.5-13.5 cm and 1.8-2.1 months for females, and 10.5-11.5 cm and 2.1-2.5 months for males, respectively. in the female worm burden 1 per case, the egglaying capacity steadily increased from 12.6 cm of their length up to 25.0 cm and then decreased significantly after th ...19902133423
a bradykinin-antagonist blocks antigen-induced airway hyperresponsiveness and inflammation in sheep.the effects of a new bradykinin-antagonist, npc-567 (d-arg [hydroxyproline3, d-phenylalanine7] bradykinin) were studied on antigen-induced airway hyperresponsiveness and inflammation in allergic sheep (n = 7). specific lung resistance (srl) was used to assess airway responses to inhaled ascaris suum-antigen. airway responsiveness was determined from slopes of cumulative dose-response-curves (drc) to inhaled carbachol. drcs were performed at baseline and 2 h after an inhalation challenge with ant ...19902135209
visceral larva migrans: a seroepidemiological survey in five municipalities of são paulo state, brazil.an enzyme-linked immunosorbent assay (elisa), employing antigens from toxocara canis larvae and the absortion of suspected sera with ascaris lumbricoides extracts was used in a seroepidemiological study performed in five municipalities of são paulo state, brazil (são paulo, campinas, santos, marília and presidente prudente) in order to determine the frequency of antibodies to toxocara. in 2,025 blood samples collected, 806 proceeded from male subjects and 1,219 from females; 483 samples were col ...19902135374
ascaris lumbricoides in neonate: evidence of congenital transmission of intestinal nematodes.clinical and epidemiological study of a forty-days-old infant with a diarrheic condition and insufficient development led to the coprological diagnosis of ascariasis and possible congenital infection. specific treatment with levamisole, resulted in clinical and parasitological cure, in addition to gain of weight up to normal levels. maternal parasitism had been diagnosed two months before labor and proved beyond doubt during the ensuing epidemiological inquiry.19902135475
parasitological and serological studies on amoebiasis and other intestinal parasitic infections in the rural sector around recife, northeast brazil.parasitological examinations were carried out during july to december, 1989, on 485 inhabitants of four villages in são lourenço da mata, 25 km northwest of recife, pernambuco, brazil. approximately 99.6% of the inhabitants were infected with at least one species of intestinal parasites. a high prevalence of schistosoma mansoni (82.1%), hookworm (80.2%) trichuris trichiura (69.9%), ascaris lumbricoides (61.9%) and entamoeba coli (36.7%) infections were demonstrated. test tube cultivation reveale ...19902135489
[allergic and heteroallergic reactions caused by the lipopolysaccharide complexes of ascarids]. 19902142992
relationship of the prevalence of swine helminths to management practices and anthelmintic treatment in danish sow herds.during 1982-1984, the occurrence of helminths in 66 sow herds in denmark was examined by means of faecal samples. the correlations between the prevalences of ascaris suum and oesophagostomum spp. and management practices were analysed for fatteners and sows, respectively, resulting in four multivariate models. the helminth prevalences were in general low in large herds, specific pathogen-free (spf) herds and herds with low weaning age. the multivariate analyses showed that only the two latter co ...19902144677
the nematode spliced leader rna participates in trans-splicing as an sm snrnp.the trans-spliced leader rna (sl rna) of nematodes resembles u snrnas both in cap structure and in the presence of a consensus sm binding site. we show here that synthetic sl rna, synthesized by in vitro transcription, is efficiently used as a spliced leader donor in trans-splicing reactions catalyzed by a cell free extract prepared from developing embryos of the parasitic nematode, ascaris lumbricoides. efficient utilization of synthetic sl rna requires a functional sm binding site. mutations w ...19902145151
the impact of inadequate sanitary conditions on health in developing countries.one of the achievements of the international drinking water supply and sanitation decade (1981-1990) is a better understanding of the relationship between poor sanitary conditions and health. this article reviews current knowledge of the major water- and excreta-related diseases and the potential impact of improved sanitary facilities on health. four health indicators are considered--diarrhoeal diseases, soil-transmitted nematode infections, dracunculiasis (guinea-worm disease) and nutritional s ...19902146815
control of ascariasis through age-targeted chemotherapy: impact of 6-monthly chemotherapeutic regimens.a field trial of 6-monthly ascariasis chemotherapeutic regimens targeted at 1-19-, 1-14-, and 5-19-year-olds was carried out in three communities in rural myanmar to observe the effects on the prevalence, intensity, and morbidity indicators over 2 years. after periodic chemotherapy, the prevalence and intensity of ascaris infection in age-targeted and non-age-targeted groups fell in all the study areas, more markedly among the 1-19- and 1-14-year-olds. there was also a decrease in the frequency ...19902150011
diploids of ovicidal fungus penicillium lanosum.by use of auxotrophic mutants of penicillium lanosum were prepared the heterokaryonts. from more rapidly growing sectors from heterokaryotic mycelium on minimal medium were isolated 17 diploid strains. they were prototrophic and on the whole they did not differ from the wild strain in their morphology and their growth rate. all diploids had considerably larger spores than the wild strain. ovicidity of one half of the diploids, as well as ovicidity of the wild strain was inconspicuous and low. ov ...19902150265
[biliary ascaridiasis in children. ultrasonic diagnosis].ascariasis is one of the most frequent intestinal parasitosis of man, specially in tropical regions where it affects mostly children. the adult worm can migrate through the ampulla of vater and penetrate the bile ducts producing different degrees of obstruction and infection. ultrasonography is a rapid and simple non invasive procedure very effective for the visualization of the worm in the biliary tree. we present the clinical and ultrasonographic characteristic of biliary ascariasis in five ch ...19902152283
[enteroparasitoses in children from the v region. a study in the juan fernandez archipelago, chile].the results of two enteroparasitological surveys in children carried out in the juan fernandez archipelago, within a 5-year period (1982-1987), are compared. in both studies the absence of ascaris lumbricoides has been persistent, having no explanation for this phenomenon due to the favorable environmental conditions for the development of the quoted parasite. in the second survey cryptosporidium sp. was studied, finding a higher frequency than that detected in rural areas of the v region.19902152352
[parasitism in fishes and human riverside communities of the huillinco and natri lakes (great island of chiloé), chile].in april and december 1989, 35 fish from lake huillinco (42 degrees 48's, 74 degrees 02'w) and 36 fish from lake natri (42 degrees 48's, 73 degrees 50'w), in the great island of chiloé (chile) were examined. coprological samples from 159 persons, 17 dogs, 19 pigs and 4 cats from around both lakes were examined for diphyllobothrium spp. infection. in the lake huillinco the following helminths of fishes were determined: contracaecum sp. and hysterothylacium sp. in salmo trutta, cauque mauleanum an ...19902152358
[new anthelmintic drugs].new anti-helminthic drugs have appeared lately: benzimidazoles (mebendazole, flubendazol, and abendazole), praziquantel and ivermectin. mebendazol and flubendazol are poorly absorbed and are effective for ascaris, oxyuriasis and trycocephalus both in adults and children. abendazole is well absorbed and may be considered the drug of choice for ascaris and oxyuriasis at a single 400 mg dose. this drug may also be used for hydatid cyst when surgery is not possible and for cysticercosis of the nervo ...19902152732
[development of the prevalence rate of four intestinal parasites in children].during the last 2 decades, the same coprological techniques for the detection of parasites have been applied by the same team in the parasitology laboratories of the children's hospital of bordeaux. a marked decrease in the prevalence of the most frequently occurring parasites (giardia and entamoeba coli cysts, ascaris and trichocephale eggs) has been observed. similar results have also been found in other french parasitology laboratories. this reduction in the parasite infection rate is concomi ...19902158059
catalase activity during the development of the parasitic nematode, ascaris suum.1. catalase activity was partially purified from body wall muscle of the parasitic nematode, ascaris suum, and was similar to catalases isolated from mammalian tissues. it exhibited a broad ph optimum and was unaffected by 2 mm ethylenediaminetetra-acetate. in contrast, it was inhibited reversibly by 1 mm cyanide and irreversibly by prior incubation in 40 mm 3-amino-1:2:4-triazole for 1 hr or heating at 80 degrees c for 15 min. 2. catalase activity was highest in the unembryonated "egg" and decr ...19902160878
increased leukotriene e4 excretion during antigen-induced bronchoconstriction in allergic sheep.the metabolism of leukotrienes (lt) in the sheep was investigated to define markers of 5-lipoxygenase involvement in allergic responses, obtainable by noninvasive techniques. intravenous administration of 14, 15-[3h]ltc4 (0.5 microci/kg) revealed a rapid clearance from the circulation (half time = 90 s). circulatory metabolism was apparent, with early formation (within 1 min) of ltd4 and lte4 shown by reverse-phase high-pressure liquid chromatography (rp-hplc). urinary 3h excretion comprised 10% ...19902161409
trans splicing of nematode pre-messenger rna in vitro.in nematodes, a fraction of mrnas contains a common 22 nucleotide 5' terminal spliced leader (sl) sequence derived by trans splicing. here, we show that a cell-free extract prepared from developing embryos of the parasitic nematode ascaris lumbricoides catalyzes accurate and efficient sl addition to a synthetic pre-mrna at an authentic trans splice acceptor site. sl addition occurs via a trans splicing reaction that proceeds through y-branched intermediates. the branchpoint is located at either ...19902163760
the effect of nedocromil and dexamethasone on the antigen induced changes of sheep tracheal smooth muscle responsiveness. 19902164748
cellular ltb4 production differs in allergic sheep with and without late airway responses.we tested the hypothesis that allergic sheep that develop both early and late airway responses to inhaled ascaris suum antigen (late responders) have an increased capacity to generate leukotrienes (lts) compared with allergic sheep that show only early responses to inhaled antigen (acute responders). to test this hypothesis, we measured ltb4 production, in vitro, by granulocytes isolated from peripheral blood and by macrophages isolated from bronchoalveolar lavage (bal) from both groups of sheep ...19902166443
ultrasonography of intestinal ascaris. 19902174929
avermectin and avermectin derivatives are antagonists at the 4-aminobutyric acid (gaba) receptor on the somatic muscle cells of ascaris; is this the site of anthelmintic action?the mechanism underlying the ability of the anthelmintic avermectin to paralyse the nematode ascaris is not yet fully understood. using conventional two-electrode electrophysiological recording techniques we have demonstrated that micromolar concentrations of ivermectin block the inhibitory gaba response on the muscle cells of the parasitic nematode ascaris. the ability of a number of avermectin derivatives to act as receptor antagonists for the ascaris muscle gaba receptor has been determined. ...19902175874
protein kinase activities during early development of ascaris suum. 19902177849
public health and research perspectives on the microbial contamination of foods.scientific advances in methodology and epidemiology have resulted in a renewed awareness of foodborne disease, and increased contact among nations of the world has stimulated rapid global distribution of foods as well as foodborne pathogens. new food vehicles are being identified for old, familiar pathogens, and new pathogens are being discovered. current research in food microbiology has spurred development of rapid and specific methods to identify these pathogens and to assess their virulence. ...19902180884
repeated antigen inhalation results in a prolonged airway eosinophilia and airway hyperresponsiveness in primates.the effects of repeated antigen inhalation on airway cellular composition and airway responsiveness were examined in primates. airway cellular composition was assessed by bronchoalveolar lavage (bal), and airway responsiveness was measured as the bronchoconstrictor response to cumulative methacholine dose-response determinations over the course of a 10-wk study. control animals, exposed to repeated vehicle inhalation challenges, were tested in parallel with the antigen-challenged group. repeated ...19902180898
use of monoclonal antibodies for the characterization of onchocerca volvulus antigens.in order to identify onchocerca volvulus antigens that could be considered as either diagnostic and/or immunoprophylactic, mouse monoclonal antibodies were produced against o. volvulus soluble antigens. three were selected on the basis of their staining patterns in an indirect fluorescent antibody assay carried out on cryosections of adult o. volvulus. the first monoclonal antibody (k1-159) recognized a cuticular antigen which appeared in ifa to be restricted to the genus onchocerca. however, ne ...19902187226
serum levels of gastrin, insulin and glucagon as possible factors of anorexia in pigs infected once with ascaris suum.in order to determine possible mediators for development of anorexia in pigs infected with ascaris suum, serum levels of gastrin, insulin and glucagon were measured. after a single high oral dose of 100,000-200,000 embryonated eggs the serum levels of gastrin and insulin in the infected pigs did not significantly differ from those in controls. serum glucagon levels in the infected groups, however, were lower than those in controls and the difference was more evident 24 days postinoculation and l ...19902205042
subacute non-bolus intestinal obstruction caused by ascaris lumbricoides. 19902206819
[clinical observations on the treatment of hookworm, ascaris and trichuris infection with oxibendazole].340 cases of hookworm infection, 196 cases of ascariasis and 178 cases of trichuriasis were treated with an anthelmintic, oxibendazole, 15mg/kg.d x 3d; 102, 70 and 66 cases of respective infections were treated with pyrantel 10mg/kg.d x 3d and 108, 74 and 63 cases took placebo for comparison and as control under double-blind observations. re-examinations of the stool were performed after the treatment. among the cases treated with oxibendazole, the egg negative conversion rates of hookworm, asca ...19902208619
[effect of albendazole and pyrantel in treating intestinal helminthiasis and controlling the recurrence of hookworm infections].a total of 720 human intestinal helminthic infections were divided into 4 groups and treated with albendazole 400mg/d x 3d, 400mg/d x 5d, pyrantel 1,500mg/d x 3d, or 1,500mg/d x 5d. half a month after treatment, the negative rates of hookworm egg were 98.6, 98.6, 86.2 and 93.5%, those of ascaris egg were 96.5, 98.2, 92.9 and 96.3%, and those of whipworm egg were 86.4, 89.0, 68.9 and 67.0% respectively. reduction rate of hookworm egg reached more than 98% in all the 4 groups. six months after tre ...19902208633
a set of trnas that lack either the t psi c arm or the dihydrouridine arm: towards a minimal trna adaptor.the mitochondrial dna (mtdna) molecules of the nematode worms, caenorhabditis elegans and ascaris suum contain 22 putative genes for non-standard forms of trnas. the inferred transcripts can be folded into 20 separate structures each resembling a trna whose t psi c arm and variable loop are replaced with a simple loop of 6-12 nucleotides. in two further structures [that resemble trnas for ser(ucn) and ser(agn)], the dihydrouridine arm is replaced by a loop of 5-8 nucleotides. by hybridizing mt-t ...19902209550
the risk approach in preschool children suffering malnutrition and intestinal parasitic infection in the city of são paulo, brazil.the association between parasitic infection and malnutrition in preschool children in the city of são paulo, was studied according to degree of malnutrition, age, and income. associations were assessed by risk analysis. higher risks of malnutrition were found in children above 24 months of age infected with ascaris, trichuris or with more than three different parasites. the presence of more than three parasites per child affected nutritional status independently of income level. it is suggested ...19902213984
effect of ascariasis upon nutritional status of children.one hundred children aged 24-72 months who had history of passing roundworms during the preceding 12 months were compared with 100 children without such history. the two groups were matched for age, sex, economic status, and birth order. children who had ascaris were 2.1 cm shorter in height, 0.6 cm, thinner for mid-arm circumference, and 0.38 kg lighter in weight compared to the children who did not pass ascaris. the first two of these differences were statistically significant (p less than 0.0 ...19902213985
[the similarity of the biological effects from the parenteral administration of ascarid antigenic complexes and salmonella typhi endotoxins].the experiment with rabbits showed that it was basically possible to develop local and generalized shwartzman's phenomenon under the combined effect of ascaris antigens and typhoid bacterial endotoxins. intracutaneous (0.2 ml.) or intravenous (0.1 ml/kg) sensitization of animals by ascaris antigens or microbe endotoxins after repeated intravenous crossover, intravenous injection of the antigens used to result in the development of classical local and generalized shwartzman's phenomenon.19902215371
ascariasis.ascaris lumbricoides is the most common helminth to infect humans. infection occurs when contaminated soil containing mature eggs is swallowed. clinically, infection ranges from the asymptomatic carrier state to life-threatening pulmonary disease or intestinal obstruction. diagnosis is most frequently established by identification of the eggs in stool specimens. treatment with antiparasitic drugs is effective in preventing serious complications and eradicating the parasite from all members of th ...19902220526
primary structure of the major pepsin inhibitor from the intestinal parasitic nematode ascaris suum.the major pepsin inhibitor from ascaris suum was isolated by affinity chromatography and chromatofocusing. its amino acid sequence was determined by automated edman degradation of peptide fragments. peptides were produced by chemical and enzymatic cleavage of pyridylethylated protein and were purified by reverse-phase high-performance liquid chromatography. the inhibitor consists of 149 residues with the following sequence: qflfsmstgp10fictvkdnqv20fvanlpwtml30egddiqvgke40 faarvedctn50vkhdmaptct6 ...19902223768
two highly reiterated nucleotide sequences in the low c-value genome of panagrellus redivivus.two families of highly reiterated satellite nucleotide (nt) sequences have been found in the genome of the sexually separated nematode panagrellus redivivus. the repeats are arranged in tandem arrays but the different satellites are not intermingled. monomeric lengths are of 155 bp for one kind and 167 bp for the other; they were named e155 and e167. the a + t content is elevated in both families (i.e., 59.5%, and 65.3%, respectively). no similarity was found between the two satellites nor to ot ...19902227434
effect of drug treatment on the prevalence of intestinal parasites amongst school children in a sub-urban community.the prevalence of intestinal protozoal and helminthic infection was studied over a period of one year amongst 297 school children, aged 5-20 yr in a sub-urban area. the overall prevalence rate of various protozoal infection, 15.82 per cent had only helminthic infection and 14.47 per cent had combined helminthic and protozoal infection. ascaris lumbricoides infestation was found to be the commonest helminthic infection (61.70%) while giardiasis was the commonest protozoal infection (59.80%). the ...19902228055
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