| schistosoma japonicum: use of a radioimmunoassay for anti egg antibodies in human sera. | a solid-phase radioimmunoassay (ria) was developed for schistosomiasis japonica using extracted egg antigens and compared with circumoval precipitin test (copt) results on 20 sera from known s. japonicum-infected individuals and on 10 control sera. the quantitative ria very clearly differentiated between infected and uninfected individuals with highest titers being obtained in teenagers. however, in the series employed, information relevant to immunodiagnosis of s. japonicum infection was contai ... | 1981 | 7196090 |
| a sieving method for the collection of schistosome eggs from mouse intestines. | | 1981 | 7196444 |
| murine hybridoma-derived antibodies producing circumoval precipitation (cop) reactions with eggs of schistosoma japonicum. | of 7 hybridomas which secrete immunoglobulins binding to crude extracts of schistosoma japonicum adult worms and/or eggs in solid-phase radioimmunoassays (rias), 3 gave positive precipitation reactions in the circumoval precipitin test (copt). the copt is a simple and inexpensive immunodiagnostic test for schistosomiasis japonica which involves the incubation of a selected batch of s. japonicum eggs with sera from patients and examination for precipitates one or more days later. using a competit ... | 1981 | 7197517 |
| fractionation of schistosoma japonicum soluble egg antigen glycoproteins by hydrophobic interaction chromatography. | we are currently studying the soluble egg antigens of schistosoma japonicum in an attempt to determine which antigens are potent immunogens. previously, we demonstrated by ouchterlony immunodiffusion and inhibition of the circumoval precipitin test that the glycoprotein fraction of soluble egg antigens contains the antigens which are most immunogenic in natural infections. the soluble egg antigen glycoproteins have now been further fractionated via hydrophobic interaction chromatography on pheny ... | 1981 | 7198100 |
| [studies on chemotherapy of parasitic helminths (iv). effects of alkaloids from sophora flavescens on the motility of parasitic helminths and isolated host tissues (author's transl)]. | effects of n-methylcytisine (n-mc) and matrine (mat), alkaloids from sophora flavescens on the motility of parasitic helminths and isolated frog rectus and mouse ileum were studied. 1) the motility of angiostrongylus cantonensis was affected spastically by n-mc (1.2 x 10(-6) approximately 1.2 x 10(-4) m), but paralytically by mat (10(-5) approximately 10(-4) m). 2) the motility of dipylidium caninum and fasciola hepatica was affected paralytically by n-mc (1.2 x 10(-4) approximately 1.2 x 10(-3) ... | 1982 | 7200047 |
| [studies on chemotherapy of parasitic helminths (iii). effects of tuberostemonine from stemona japonica on the motility of parasitic helminths and isolated host tissues (author's transl)]. | effects of tuberostemonine (ts), an alkaloid from stemona japonica, on the motility of parasitic helminths and isolated frog rectus and mouse ileum were studied. 1) ts (6.7 x 10(-6) approximately 2 x 10(-5) m) paralyzed the motility of angiostrongylus cantonensis. 2) ts (6.7 x 10(-5) m) showed contractive effects on the motility of dipylidium caninum and fasciola hepatica. 3) treatment with ts (6.7 x 10(-5) approximately 4.8 x 10(-4) m) had little effect on the motility of schistosoma japonicum. ... | 1982 | 7200048 |
| a comparative study of the vitelline cell in schistosoma mansoni, s. haematobium, s. japonicum and s. mattheei. | a comparison is given of the ultrastructure of the vitelline cell in schistosoma mansoni, s. haematobium, s. japonicum and s. mattheei. four stages in development of the vitelline cell have been categorized as follows: stage 1, the undifferentiated cell; stage 2, the developing cell showing the beginning of synthetic activity; stage 3, the developing cell showing active protein synthesis; stage 4, the fully mature vitelline cell. these stages in development have been defined morphologically and ... | 1982 | 7200223 |
| annual fluctuation of schistosoma japonicum infection in field rats, rattus rattus mindanensis, in dagami, leyte, philippines. | the annual fluctuation of schistosoma japonicum infection in field rats, rattus rattus mindanensis, in leyte, the philippines was investigated. one hundrend live-traps were set for one week every month from february, 1978 to february, 1979, in and around central elementary school ii, diagami, leyte. out of 199 rats, 163 (81.9%) were infected. the prevalence rate of s. japonicum infecton in rats was high during the relatively dry season, april to july, 1978. meanwhile, the prevalence rate of schi ... | 1980 | 7206265 |
| [studies on the effectiveness and toxicity of a niridazole derivative s 72014 in experimental chemotherapy of schistosomiasis japonica (author's transl)]. | | 1980 | 7211389 |
| schistosoma japonicum-like eggs in the appendix of an inhabitant of java, indonesia. a case report. | the second case of autochthonous schistosoma japonicum-like infection in man from indonesia is reported. an appendectomy was performed on a 34-year-old inhabitant of java of chinese heritage. microscopic examination of the necrotic appendix revealed s. japonicum-like eggs in the mucosa, submucosa, muscularis, and serosa layers. there were many degenerated and mineralized eggs, some of which were within giant cells and/or surrounded by scar tissue. | 1981 | 7212176 |
| the emergence of schistosoma japonicum cercariae from oncomelania quadrasi. | the release of schistosoma japonicum cercariae from leytean oncomelania quadrasi snails was observed under laboratory conditions. two patterns of emergence were noted. the initial, nonperiodic emergence occurred immediately after submerging the snails in water and was followed by a periodic, diurnal emergence which peaked in the afternoon. the daily cercarial output of the periodic emergence appeared to be affected by exogenous light intensity. furthermore, there was a cessation or reduction in ... | 1980 | 7218093 |
| geographical factors influencing the population numbers and distribution of oncomelania nosophora and the subsequent effect on the control of schistosomiasis japonica in japan. | | 1981 | 7221601 |
| intestinal and blood parasites in the torro valley, central sulawesi, indonesia. | approximately 300 blood and fecal specimens were examined in a parasitologic survey of indigenous inhabitants of the small isolated torro valley in the mountains of central sulawesi. schistosoma japonicum was not found although the parasite is endemic in the neighboring lindu and napu valleys. hookworm infection (71%) was the most common helminthiasis. the prevalences of ascariasis (3%) and trichuriasis (2%) are low for indonesia in general but similar to those found in nearby mountainous areas ... | 1980 | 7221687 |
| intestinal parasites of man in northern bohol, philippines, with emphasis on schistosomiasis. | a survey for intestinal parasites with emphasis on oriental schistosomiasis was conducted in the townships of trinidad and talibon, bohol province, philippines and approximately 1,700 stool samples were examined. schistosoma japonicum is still endemic to these areas of northern bohol but infection rates were much lower than expected, 5% rather than 30%. soil-transmitted helminths were the most common parasitic infections. hookworms were found in 71% of the samples tested, most infections were du ... | 1980 | 7221688 |
| [cor pulmonale caused by chronic schistosomiasis japonica (author's transl)]. | | 1980 | 7227072 |
| kinetics and mechanisms of pulmonary granuloma formation around schistosoma japonicum eggs injected into mice. | | 1981 | 7237566 |
| [synthesis of 4-pyridine-acrylic acid derivatives (author's transl)]. | | 1981 | 7246163 |
| intestinal parasites of man in oriental mindoro, philippines, with emphasis on schistosomiasis. | an intestinal parasite survey was conducted in the town of victoria, oriental mindoro, philippines in 1978 in order to estimate the current schistosomiasis situation in this area of the philippines and schistosoma japonicum eggs were detected in 20% of 1058 fecal specimens examined. the prevalence was more than twice as high as had been previously estimated. infection rates were higher in males than in females, 26 versus 15% and age-wise were infrequent in young children (8%) and moderately comm ... | 1981 | 7256349 |
| intestinal parasites of man in bukidnon, philippines, with emphasis on schistosomiasis. | a total of 831 fecal specimens from malay-balay, mindanao, philippines were examined for schistosomiasis and common intestinal parasites. schistosoma japonicum eggs were found in 16% of the samples. infections were more common in males (20%) than in females (12%). age-wise, infections were infrequently diagnosed in children less than 10 years and infections in older age groups varied from 12-27%. hookworm, chiefly necator americanus, was the most common parasite found (44%) followed by ascaris l ... | 1981 | 7256354 |
| [an autopsy case of cryptococcal meningitis with chronic schistosomiasis, japonica (author's transl)]. | | 1981 | 7261504 |
| schistosoma japonicum: infection characteristics in mice of various strains and a difference in the response to eggs. | | 1981 | 7287292 |
| population studies on oncomelania quadrasi, the snail intermediate host of schistosoma japonicum, in the philippines. i. distribution pattern of the snail in the field. | distribution of oncomelania quadrasi, the snail intermediate host of schistosoma japonicum, was studied in leyte, philippines for the type of distribution based upon a large number of survey data. among four distribution models examined, three uneven distribution models, i.e., the double poisson, neyman type a and negative binomial fitted well in this order but the poisson model showed the poorest fitness. this indicates that the snails are distributed unevenly over the field and that the popula ... | 1981 | 7300032 |
| [coadministration of pyquiton and furapromidum in the treatment of experimental schistosomiasis japonica (author's transl)]. | | 1981 | 7304183 |
| [the effect of pyquiton on the uptake and release of 5-hydroxytryptamine in schistosoma japonicum (author's transl)]. | | 1981 | 7304187 |
| susceptibility of oncomelania hupensis formosana recombinants and hybrids with oncomelania hupensis nosophora to infection with schistosoma japonicum. | | 1981 | 7309340 |
| infection with schistosoma mekongi in southeast asian refugees. | schistosoma mekongi is a newly recognized species of schistosoma that is closely related to schistosoma japonicum and is pathogenic to humans. like the other forms of schistosomiasis in which the parasites reside in the mesenteric vasculature, s. mekongi infection has hepatosplenomegaly and portal venous hypertension as its most common serious sequelae. schistosomiasis, although endemic in certain areas of southeast asia, has not been recognized as a health problem in asian refugees who are curr ... | 1981 | 7310175 |
| schistosoma japonicum: ultrastructural changes in the tegument during cercaria-schistosomulum transformation. | the tegumental changes of schistosoma japonicum cercaria were initiated when introduced into nctc 109 containing antibiotics for axenizing. in some regions the tegument was covered with a penta- or heptalaminate membrane, and the surface coat changed into an electron-dense, amorphous material. large vacuoles limited by a multilaminate membrane were evident in the tegumental cytoplasm, and some appeared to join or open to the tegument. after 4 h incubation in nctc 109 containing 50% fresh rabbit ... | 1981 | 7311078 |
| histological changes elicited in schistosoma japonicum infected rabbits following curative chemotherapy with 4-isothiocyano-4'-nitro-diphenylamine (c9333-go/cgp 4540). | new zealand white rabbits were infected with 250 or 500 cercariae of the philippine-leyte strain of schistosoma japonicum. 26 weeks later the experimental group were given one dose of 25 mg/kg formulated cgp 4540. six weeks after dosing the treated rabbits and untreated controls were killed and examined. parasitological cure was complete in all those treated. the liver and intestinal granulomata were greatly diminished in size and there was a dramatic decrease in the number of inflammatory cells ... | 1981 | 7324109 |
| [studies on chemotherapeutic agents against schistosomiasis japonica. i. synthesis of alcoholic and phenolic esters of monobromoacetic acid]. | | 1981 | 7324962 |
| [experimental therapy and mechanism of action of nithiocyamine on schistosoma japonica]. | | 1981 | 7331811 |
| [studies on chemotherapeutic agents against schistosomiasis japonica. ii. synthesis of coumarin esters of monobromo- and monoiodoacetic acid (author's transl)]. | | 1981 | 7336940 |
| population studies on oncomelania quadrasi, the snail intermediate host of schistosoma japonicum, in the philippines. 2. necessary sample size for snail density survey. | in order to save time and manpower for the density surveys of oncomelania quadrasi, the snail host of schistosoma japonicum in the philippines, the minimal necessary sample size (q) was determined using a formula, q greater than t2/e2(1d - 1 + 1/-x). this formula is based on the id dispersion index [2, 3] which varies with the degree of clumping of unevenly distributed animals. a nomograph was prepared for the ready determination of the necessary sample size for various degrees of clumping (id) ... | 1981 | 7339070 |
| attempts to standardize the circumoval precipitin test (copt) for schistosomiasis japonica. | the circumoval precipition test (copt) is a simple and inexpensive immunodiagnostic test for schistosomiasis japonica which, in the philippines, has high sensitivity and specificity. lack of standardization does, however, increase the variability of the test. parameters which influence the copt have been examined using large numbers of sera from known s. japonicum infected individuals. in this series of experiments, optimal conditions were determined to be as follows using 2 drops of neat serum ... | 1981 | 7342326 |
| [general review of the clinical results obtained with biltricide in the treatment of human schistosomiasis (author's transl)]. | | 1981 | 7343126 |
| prevalence of antibodies to schistosoma japonicum in the chikugo river basin. | | 1981 | 7343760 |
| [hepatosplenic disorders in schistosomiasis (author's transl)]. | hepatosplenic disorders start in the invasion stage and are from immuno-allergic origin. then, they develop during the ovular laying stage; hepatic disorders are due to periovular granulomatosis, and splenic disorders are due to reticulo-endothelial system reaction. their last aspect is the portal fibrosis, called symmer's "clay pipe", in which htp is consequent to hepatic disorder and the main cause of splenomegaly. the location of s. japonicum and s. mansoni in pericolic veins explains that he ... | 1980 | 7366366 |
| [anatomopathological aspects of schistosomiasis. a study of 286 pathological specimens. (author's transl)]. | schistosomiasis pathology demonstrates the successive stages of the specific inflammation and accounts for the large variety of its macroscopic and histological features. this paper reviews 286 bilharzial lesions studied between 1956 and 1979 at the ""institut de médecine tropicale du service de santé des armées". - first, the histopathological caracteristics of the disease are considered. some lesions are caused by schistosomules and adult worms, but the proeminent role is devoted to egg: the b ... | 1980 | 7366368 |
| [medical treatment of schistosomiasis (author's transl)]. | after a brief reassessment of the pathogenic problem, the authors review the presently available drugs and their respective dosage and side-effects. then they consider each of the four parasitological types of schistosomiasis and indicate the most active treatment. | 1980 | 7402062 |
| [pulmonary symptoms in schistosomiasis (author's transl)]. | | 1980 | 7402063 |
| report of a freshwater hydrobiid snail from pahang, malaysia: a possible host for schistosomes infecting man. | | 1980 | 7403949 |
| [studies on the diagnosis of schistosomiasis japonica. (3) an immediate intradermal test on the inhabitants of an endemic area and an epidemiologic study based on the analysis of a dermal reaction (author's transl)]. | the intradermal test of this disease was performed by injecting 0.02 ml of the antigen into the middle third of the volar surface of the forearm; the reaction was judged after 15 minutes and a diameter of 9 mm or more in wheal and/or 20 mm or more in erythema were regarded as the positives. the following results are presented on this basis. (1) the percentages of the dermal reactors for the subgroup by age were shown in table 1. age was important in the sensitivity of the intradermal test with s ... | 1980 | 7409733 |
| the energy metabolism of schistosoma japonicum. | | 1980 | 7418939 |
| [efficacy and toxicity of an indane compound, s75029 in experimental therapy of schistosomiasis japonica (author's transl)]. | | 1980 | 7424532 |
| [bilharziasis; a schistosomal infection]. | | 1980 | 7429386 |
| discovery of a site of transmission and hosts of a schistosoma japonicum-like schistosome in peninsular malaysia. | | 1980 | 7434446 |
| morbidity in schistosomiasis japonica in relation to intensity of infection: study of a community in leyte, philippines. | | 1980 | 7435790 |
| lymphocyte response in mice infected with the indonesian and formosan strains of schistosoma japonicum to concanavalin a, and to worm and egg antigens. | cultures of splenocytes from mice infected with the formosan and indonesian strains of schistosoma japonicum demonstrated a blastogenic response to concanavalin a (con-a), adult worm antigen (saa) and soluble egg antigen (sea). the incorporation of tritiated thymidine was significantly greater for lymphocytes from mice infected with the pathogenic indonesian strain than for lymphocytes from mice infected with the non-pathogenic formosan strain in response to saa; the responses also increased wit ... | 1980 | 7444571 |
| susceptibility of new taiwan foci of oncomelania hupensis to geographic strains of schistosoma japonicum. | six new recently identified taiwan oncomelania hupensis foci were exposed to miracidia from the changhua taiwan zoophilic and indonesian and philippine anthropophilic strains of schistosoma japonicum. all new foci were found susceptible to infection in varying degrees. in general the new foci were more susceptible to the changhua zoophilic strain of s. japonicum than the anthropophilic strains; 17% exposed to the zoophilic strain shed cercariae while 9% and 7%, respectively, of those exposed to ... | 1980 | 7444579 |
| analysis of the immunoglobulins responsible for the circumoval precipitation reaction. | two morphologically distinct types of circumoval precipitates (cop) have been observed in human schistosoma japonicum infections. an elongated segmented cop occurs in chronic human infections. an unsegmented "reaction of recent infection" (rri) occurs in serum from humans with recently acquired infections and is morphologically similar to the reaction observed in the sera of mice infected with s. japonicum. sera from infected mice and humans were separated by g-200 chromatography to determine wh ... | 1980 | 7446814 |
| the production and nature of anemia in schistosoma japonicum infections. | | 1980 | 7446821 |
| quantitative parasitologic findings in rabbits infected with japanese and philippine strains of schistosoma japonicum. | sixty-seven rabbits were killed 8-66 weeks after infection with a japanese strain of schistosoma japonicum and 22 were killed 12-55 weeks after infection with a philippine strain. worm numbers decreased moderately over the course of infection and the number of eggs laid by each worm pair also decreased, as evidenced by decreasing numbers of mature (viable) eggs per worm pair in the tissues and, for the japanese strain, by decreasing numbers of eggs per worm pair passed in the feces. many eggs ca ... | 1980 | 7446822 |
| extrahepatic pathology in rabbits infected with japanese and philippine strains of schistosoma japonicum, and the relation of intestinal lesions to passage of eggs in the feces. | a japanese strain of schistosoma japonicum produced segmental circumferential lesions 15-40 cm in length in the proximal jejuum of infected rabbits, while a philippine strain of the parasite produced small numbers of focal nodular lesions (bilharziomas) in the colon. sequestration of large numbers of schistosome eggs in these latter lesions apparently accounted for the small and erratic number of s. japonicum eggs passed in the feces of rabbits infected with the philippine strain. these focal ma ... | 1980 | 7446823 |
| [an experimental study on the effect of nithiocyamine against schistosoma japonicum (author's transl)]. | | 1980 | 7470232 |
| schistosoma japonicum, zoophilic strain, in oncomelania hupensis chiui and o. h. formosana: miracidial penetration and comparative histology. | miracidial penetration and intramolluscan development of a zoophilic strain of schistosoma japonicum were studied in the highly susceptible oncomelania hupensis chiui and a highly resistant population of oncomelania hupensis formosana. when exposed to 20 miracidia, an average of 3.6 miracidia penetrated o. h. chiui and 3.4 penetrated o. h. formosana. the distribution of sporocysts in the 2 snail subspecies was similar: 70.4% migrated to the cephalopedal sinus, 14.2% to the anterior viscera, and ... | 1995 | 7472860 |
| purification of recombinant rho/rac/g25k from escherichia coli. | | 1995 | 7476445 |
| immunization of mice with recombinant sjc26gst induces a pronounced anti-fecundity effect after experimental infection with chinese schistosoma japonicum. | we report the cloning, by polymerase chain reaction (pcr), of a cdna encoding a schistosoma japonicum (chinese) 26 kda glutathione-s-transferase (gst) (sjc26gst), expression of the cdna, affinity purification of the recombinant gst and its vaccine efficacy in outbred nih mice using freund's as adjuvant. the most striking feature of the vaccination experiments was the pronounced reduction in the number of eggs in the livers and spleens of immunized mice. a relatively low but significant level of ... | 1995 | 7483782 |
| comparative phenotypic analysis of lymph node cells in mice after infection or vaccination with normal or ultraviolet-attenuated cercariae of schistosoma japonicum or s. mansoni. | mice were infected with 200 untreated or vaccinated with 500 ultraviolet-attenuated cercariae of either schistosoma japonicum or s. mansoni. for three weeks, cell numbers in axillary and mediastinal lymphnodes were counted and cell populations typed by cytofluorometry. in the axillary lymphnodes, numbers of b-cells and cd3+cd4+ t-cells but not cd3+cd8+ t-cells increased. following vaccination with either species, parasite migration was apparently delayed in the skin and interrupted at the lungs, ... | 1995 | 7501424 |
| comparison of the patterns of codon usage and bias between brugia, echinococcus, onchocerca and schistosoma species. | patterns of codon usage and bias were compared among taxa of the genera brugia, echinococcus, onchocerca and schistosoma by metric multidimensional scaling and three commonly used indices of bias: nc, gc3s and b. the overall codon usage for each taxon was compared, as was the codon usage for each individual gene within the taxa. differences in the patterns of codon usage observed between taxa were dependent on the overall base composition of the genes analysed. the codon usage of echinococcus wa ... | 1995 | 7501637 |
| serum levels of circulating anodic antigen and circulating cathodic antigen detected in mice infected with schistosoma japonicum or s. mansoni. | serum concentrations of circulating anodic antigen (caa) and circulating cathodic antigen (cca) were studied in mice infected with either schistosoma japonicum or s. mansoni cercariae. sera from uninfected mice were negative for both antigens. caa was detectable in the s. japonicum-infected mice as early as at 2 weeks post-infection (p.i.), and levels were higher in these animals than in the s. mansoni-infected group during the full study period. at the moment of perfusion, 10 weeks p.i., a medi ... | 1995 | 7501644 |
| hepatic fibrosis and gene expression changes induced by praziquantel treatment during immune modulation of schistosoma japonicum infection. | in the present study fibrogenic gene expression was determined in murine schistosoma japonicum infection during the progression of immune modulation of infection and following chemotherapy during the course of immune modulation. histomorphometric analysis of granuloma size and collagen deposition revealed peak granuloma size in acute infection (5 weeks) and peak hepatic collagen content at 16 weeks of infection. peak type i collagen gene expression was concomitant with tgf-beta 1 gene expression ... | 1993 | 7506405 |
| retention of a mutagen, 3-amino-1-methyl-5h-pyrido[4,3-b]indole (trp-p-2), in the liver of mice infected with schistosoma japonicum. | regarding the mechanism underlying the suspected enhancement of hepatic cancers among schistosoma japonicum-infected humans, we hypothesized that mutagen exposures in the livers of patients may be enhanced due to the parasitic infection. to explore this possibility, we have done a model experiment using mice and a carcinogenic mutagen, 3-amino-1-methyl-5h-pyrido[4,3-b]indole (trp-p-2). mice infected with schistosoma japonicum were intravenously administered trp-p-2, and the mutagenic activities ... | 1994 | 7510037 |
| parasite infection and cancer: with special emphasis on schistosoma japonicum infections (trematoda). a review. | this article contains a review of current knowledge on the association of parasite infections and cancer formation, especially that of schistosoma japonicum (trematoda) in man and experimental animals. the association of s. haematobium infection and bladder cancer is well known and documented. however, s. japonicum infection has also been reported to be associated with cancer, in this case hepatocellular carcinoma and/or colorectal cancer. pathological records and analyses have shown a correlati ... | 1994 | 7510038 |
| [schistosoma japonicum adult worm rna isolation with chomczynski's method]. | a rapid method of total rna of schistosoma japonicum adult worm isolation by single step extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. the method provides a pure preparation of intact rna and can be completed within several hours. it is particularly useful for isolation of rna from minute quantity of parasite material. analysis of agarose gels electrophoresis reveals that the rna preparations show a distinct band comigrating with 18s rat brain rna and wi ... | 1993 | 7513622 |
| [studies on immunoenzymatic staining technique using ultrasonicated dry egg section on pvc membrane for diagnosis of schistosomiasis japonica]. | immunoenzymatic staining technique (iest) using pvc-membrane coated with schistosoma japonicum ultrasonicated dry egg (ude) and liver egg pvc-iest was carried out to detect specific antibodies in sera from 159 cases with schistosomiasis japonica, the positive rates being 98.1% (156/159) and 97.5% (155/159), respectively. the sera from 63 healthy individuals showed false positive reaction in 2 cases and 1 case, respectively, suggesting that the sensitivity and specificity of the two tests were si ... | 1993 | 7521803 |
| comparative analysis of full-length antigen ii/3 from echinococcus multilocularis and e. granulosus. | the recombinant echinococcus multilocularis antigen ii/3-10 is one of the most promising tools for immunodiagnosis of alveolar echinococcosis in human patients. its nucleic acid sequence represents a part of the e. multilocularis gene encoding the metacestode antigen ii/3, the former being basically present and expressed in both e. multilocularis and e. granulosus. most (94%) patients with alveolar echinococcosis respond to infection with a marked anti-ii/3-10 igg synthesis; in contrast, most of ... | 1994 | 7521956 |
| [analysis of the target epitopes recognized by two monoclonal antibodies directed to egg-associated fractions of schistosoma japonicum]. | two igm isotype monoclonal antibodies (mcab), 2h10 and 2h1, recognizing repetitive epitopes on schistosoma egg-associated molecules were characterized and their specificities were identified in a two-site sandwich elisa system. in consistent with the differences in immunological behaviour and specificity demonstrated with immunoelectrophoresis (iep) and immunofluorescent antibody (ifa) techniques, absolutely negative reactions found in the heterologous detecting system with alternated capture an ... | 1994 | 7525106 |
| immunogenicity of genetically engineered glutathione s-transferase fusion proteins containing a t-cell epitope from diphtheria toxin. | glutathione s-transferase (gst) has been shown to induce a marginal antibody response in experimental animals as well as partial protection against a number of parasitic worms, including schistosoma and fasciola species. the objective of our study was to increase the immunogenicity of gst by adding heterologous t-cell epitopes at the carboxy terminus of the protein. we generated recombinant gst proteins by attaching one or three tandem repeats of a t-cell epitope of crm197, a nontoxic variant of ... | 1995 | 7534277 |
| three-dimensional structure of schistosoma japonicum glutathione s-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from hiv. | the 3-dimensional crystal structure of glutathione s-transferase (gst) of schistosoma japonicum (sj) fused with a conserved neutralizing epitope on gp41 (glycoprotein, 41 kda) of human immunodeficiency virus type 1 (hiv-1) (muster t et al., 1993, j virol 67:6642-6647) was determined at 2.5 a resolution. the structure of the 3-3 isozyme rat gst of the mu gene class (ji x, zhang p, armstrong rn, gilliland gl, 1992, biochemistry 31:10169-10184) was used as a molecular replacement model. the structu ... | 1994 | 7538846 |
| [a preliminary report on diagnostic complementarity of gut-associated and egg-associated antigenemia in schistosomiasis japonica]. | with hyroxylapatite purified preparations and bach (biotin aminocapryl hydrazide) biotinylated mcabs, 274-2h10 and 273-2h1, recognizing different egg-associated epitopes, biotin-avidin (ba) involved alkaline phosphatase (ap) elisa with detecting sensitivities reaching nanogram levels (10(-9), were set up. the detectable limit for crude preparations of schistosoma japonicum seaj-tca in 2h10-elisa achieved 1. 0.3. 2 ng/ml, in which only s. japonicum specific egg antigens were efficiently detected, ... | 1995 | 7540518 |
| [study on adjuvant effect of schistosoma japonicum irna]. | the adjuvant effect of schistosome irna on the schistosome antigen was studied. the results by using elisa showed that the antibody level in mice immunized with s irna + swa was significantly higher than in those only immunized with s irna or swa. after cercariae challenge infection, mice immunized with s irna + swa or s irna had higher worm reduction rate. however, there were no significant differences between the two groups of mice in terms of worm reduction rate. these results suggested that ... | 1995 | 7540519 |
| induction of protective immunity to schistosomiasis with immunologically cross-reactive lumbricus molecules. | immunologically cross-reactive molecules of schistosoma japonicum and lumbricus terrestris were identified by antibodies derived from human and rodent sera. pooled igg from schistosomiasis patients but not uninfected individuals bound multiple antigens of identical molecular weight in both soluble s. japonicum worm antigen preparations (swap) and soluble earthworm preparations (sewp). these antigens had molecular weights corresponding to 18, 40, 62, 64, 74, 97, and > 110 kda. three of these anti ... | 1995 | 7543459 |
| [isolation and expression of recombinant diagnostic antigen gene of schistosoma japonicum]. | the sera of patients with chronic infection of schistosoma japonicum (sj) were used for screening the cdna expression library constructed from sj adult worm, and an antigen-positive clone sjgt4 which expressed an 148 kd fusion protein was identified. the inserted sj cdna fragment was amplified by pcr and subcloned into the vector pgex with high level of expression. a positive clone sjpg5 which expressed a fusion protein with a molecular weight of 58 kd was obtained. when the fusion protein was u ... | 1995 | 7553141 |
| [a ten-year follow-up observation on serum antibody level in schistosomiasis patients treated with praziquantel]. | the detection of the serum antibody in 122 schistosomiasis patients, using enzyme linked immunosorbent assay (elisa), had been carried out from 1980-1989. the results showed that the potency of serum antibody was on an annual progressive decrease. before treatment, the rate of elisa reactive titre above 1:800 was 87.7%, while after treatment it was 21.2% in the first year and 1% in the second year and even lower in the following years. the antibody negative conversion rate with elisa from the fi ... | 1995 | 7554166 |
| the intergenic region between the divergently transcribed niia and niad genes of aspergillus nidulans contains multiple nira binding sites which act bidirectionally. | the niad and niia genes of aspergillus nidulans, which code, respectively, for nitrate and nitrite reductases, are divergently transcribed, and their atgs are separated by 1,200 bp. the genes are under the control of the positively acting nira transcription factor, which mediates nitrate induction. the dna binding domain of nira was expressed as a fusion protein with the glutathione s-transferase of schistosoma japonicum. gel shift and footprint experiments have shown that in the intergenic regi ... | 1995 | 7565720 |
| distant strains of the fish rhabdovirus vhsv maintain a sixth functional cistron which codes for a nonstructural protein of unknown function. | we used direct rna sequencing to determine the genomic organization of the region downstream from the g gene of viral hemorrhagic septicemia virus (vhsv), a fish rhabdovirus. this region contains a gene coding for a protein, identified as nonvirion protein (nv), and the gene coding for the rna polymerase (l). thus, vhsv genome organization was confirmed to be 3'-n-p-m-g-nv-l-5'. in both a virulent european (07-71) and an avirulent north american (makah) strain, the nv gene is transcribed into a ... | 1995 | 7571446 |
| cloning and characterization of the schistosoma japonicum aspartic proteinase involved in hemoglobin degradation. | a cdna encoding a schistosoma japonicum aspartic proteinase was cloned, sequenced, and found to encode a zymogen of 380 amino acid residues, and its gene was shown to be present as a single copy in the s. japonicum genome. identity comparisons showed that the enzyme (sjpasp) was most closely related to the cathepsin ds. the deduced amino acid sequence has four potential glycosylation sites, two of which are in identical positions to the two glycosylation sites of human kidney lysosomal cathepsin ... | 1995 | 7592666 |
| imported cns schistosomiasis--a case report. | central nervous system (cns) involvement may occur in chronic schistosomiasis. it can be produced by any schistosome species but happens most frequently in chronic schistosoma japonicum infection. cns involvement by s. mansoni is relatively rare but it may occur by embolization of eggs or ectopic migration of adult worms. a case of cerebral schistosomiasis caused by s. mansoni in a 40-year-old man, who had worked in yemen, is reported. biopsies taken from the cerebellar vermis and the roof of th ... | 1995 | 7598827 |
| humoral response to a carboxyl-terminal region of the merozoite surface protein-1 plays a predominant role in controlling blood-stage infection in rodent malaria. | the developmental stages of malaria parasites that infect e are responsible for the morbidity and mortality associated with this disease. one of the leading candidates for a blood-stage vaccine against malaria is a surface protein of merozoites, the infectious stages for e, designated merozoite surface protein-1 (msp-1). the rodent malarial parasite plasmodium yoelii yoelii (py) has provided a model system for the study of this ag, and previous studies from our laboratory had demonstrated that t ... | 1995 | 7602100 |
| variable effects of maturity-onset-diabetes-of-youth (mody)-associated glucokinase mutations on substrate interactions and stability of the enzyme. | mutations in the human glucokinase (gk) gene are thought to cause maturity-onset diabetes of youth (mody) by leading to the production of enzymes with reduced catalytic activities and increased glucose km values. however, in some cases the diabetic phenotype is more severe than might be predicted from these apparent kinetic effects alone. to determine whether these mutations might also effect other characteristics of the enzyme, nine mody-associated mutants were expressed as fusion proteins with ... | 1995 | 7619052 |
| schistosoma japonicum and s. mansoni: comparison of larval migration patterns in mice. | mice were infected percutaneously with cercariae of schistosoma japonicum or s. mansoni and parasites recovered by tissue-mincing from the skin or lungs or by perfusion of the mesenteric veins. s. japonicum had a narrow peak of recovery (up to 30%) from the lungs 3 days after infection, whereas lung recovery of s. mansoni peaked only on day 6 and levelled off during the following week. infection with s. japonicum induced lung petechiae, but only after most of the parasites had left the lungs. th ... | 1995 | 7622787 |
| antibodies to schistosoma japonicum (asian bloodfluke) paramyosin induced by nucleic acid vaccination. | nucleic acid vaccination by intramuscular or intradermal delivery of dna plasmids encoding antigenic proteins has been shown to confer protection in experimental animals against viruses and unicellular protozoan parasites. however, this revolutionary approach has not been tested for induction of immunity to multicellular parasites, such as trematode worms. we report here, for the first time, that murine antibodies can be induced by intramuscular injection with plasmid dna encoding fragments of s ... | 1995 | 7626089 |
| aldehyde fixation dramatically alters the immunolocalization pattern of paramyosin in platyhelminth parasites. | | 1995 | 7628562 |
| cloning of a schistosoma japonicum gene encoding an antigen with homology to calreticulin. | a recombinant lambda gt11 clone, ivgs3, encoding part of a 55-kda antigen was isolated from an adult schistosoma japonicum cdna library. the protein expressed by this clone was recognised strongly by serum from rats that had been vaccinated with irradiated cercariae (vrs) rendering them highly immune to a challenge infection. antibodies in vrs which were specific for ivgs3 did not recognise adult worm antigens of s. mansoni, suggesting that the recombinant antigen contains species-specific epito ... | 1995 | 7630385 |
| [immunohistochemical study of epidermal growth factor in liver of mice infected with schistosoma japonicum]. | in this immunohistochemical study we examined the expression and localization of the epidermal growth factor in liver of mice infected with schistosoma japonicum during regulatory phase of immunology and early phase of hepatic fibrosis. egf immunostaining is localized along sinusoids and within cytoplasm of centrilobular area and around ova granuloma at 6,8 weeks of infection. proliferation of ito cells and collagen deposition in disse space were found by electron microscope. the data led us to ... | 1995 | 7656703 |
| scanning electron microscope observation on tegumental damage of 21-d-old schistosoma japonicum induced by praziquantel. | to study the effect of praziquantel (pra) on the tegument of 21-d-old schistosomula, mice infected with schistosoma japonicum cercariae for 21 d were treated ig with pra at a single dose. | 1995 | 7660827 |
| a procedure for the generation and the purification of escherichia coli thioredoxins with variable n-terminal sequences. | we have developed a rapid and simple procedure for the production and the purification of escherichia coli thioredoxins containing additional amino acid residues at the n-terminus. by the polymerase chain reaction, the complete gene encoding for e. coli thioredoxin was modified and amplified with the addition at its 5' end of a bamhi cloning site and a triplet coding for an arginine residue instead of the initiator methionine codon, whereas at the 3' end the stop codon was followed by an ecori c ... | 1995 | 7663153 |
| molecular phylogenetics of the four schistosoma species groups determined with partial 28s ribosomal rna gene sequences. | partial 28s ribosomal rna (rrna) gene sequences, including the variable domains d1, d2 and d3, were determined for representative species from the 4 schistosoma species groups. on an alignment of 1345 bp from s. mansoni, s. haematobium, s. spindale and s. japonicum (with heterobilharzia americana chosen as an outgroup), both maximum likelihood and maximum parsimony analyses provide a robust molecular phylogeny for the genus; ((((s. haematobium, s. spindale), s. mansoni), s. japonicum), h. americ ... | 1995 | 7675531 |
| schistosoma japonicum: heterogeneity in paramyosin genes. | paramyosin is an integral muscle protein found in many invertebrates including schistosomes, and is considered an important candidate vaccine antigen in schistosomiasis. the characterisation of natural molecular variation in vaccine antigens including paramyosin is important because strain-specific vaccination may be necessary against schistosomiasis japonica. we have isolated partial cdnas encoding paramyosin from an adult, chinese strain schistosoma japonicum gene library. two of these cdnas ( ... | 1995 | 7676904 |
| gene cloning and complete nucleotide sequence of philippine schistosoma japonicum paramyosin. | the development of an effective vaccine is recognised as a necessary adjunct to the control of schistosomiasis japonica, a disease affecting several million people in china and the philippines. currently, recombinant schistosoma japonicum molecules are considered most suitable for large scale vaccine production and a number of genes encoding vaccine candidate polypeptides have been cloned and expressed (see waine et al., 1993a). one of the molecules providing most promise as a vaccine target is ... | 1995 | 7676905 |
| experimental infection of danish landrace/yorkshire crossbred pigs with schistosoma japonicum from the people's republic of china. | this study was undertaken to assess the suitability of danish landrace/yorkshire (l/y) crossbred pigs as experimental hosts of a chinese mainland strain of schistosoma japonicum. pigs were exposed to 200, 500 or 1000 cercariae and parasite burdens were determined by perfusion after either 8 or 11 weeks. all pigs became infected with onset of faecal egg excretion 6 to 7 weeks following exposure to cercariae. the pattern of faecal egg excretion differed markedly among the individual animals. gross ... | 1994 | 7676923 |
| sequence analysis of a fasciola hepatica glutathione s-transferase cdna clone. | a lambda gt11 fasciola hepatica cdna library was previously constructed from poly(a)+ rna extracted from adult worms. a cdna encoding f. hepatica glutathione s-transferase (fhgst) (genbank accession number m93434) was cloned by screening the library with a rabbit anti-fhgst antiserum. the fhgsts are suspected to be early developmentally expressed-proteins that act as potent immunogens and may be useful as candidate vaccines against the parasite. the cdna was sequenced and contains 627 translated ... | 1993 | 7682383 |
| schistosoma: a 200-kda chemotherapeutic target antigen is differentially localized in african vs oriental species. | a 200-kda protein of the african schistosome schistosoma mansoni has been identified as a target of antibodies that act in synergy with praziquantel. treatment of worms with praziquantel exposes selective epitopes of the 200-kda protein on the surface of s. mansoni and transfer of a monoclonal antibody recognizing the 200-kda protein at the time of drug treatment restores the effectiveness of praziquantel against infections in b-cell-depleted mice. in the present study, a cross-reactive 200-kda ... | 1993 | 7684706 |
| chronic hepatitis in experimental schistosomiasis. | histological features of chronic active and chronic persistent hepatitis were observed in mice, rabbits and non-human primates infected with either schistosoma mansoni and schistosoma japonicum. in early infection hepatitis appeared as a reactive change due to liver damage caused by the deposition of schistosome eggs, but portal and septal cellular infiltrations tended to remain long after parasite aggression had diminished or disappeared, either spontaneously with time or after chemotherapy. in ... | 1995 | 7704327 |
| interaction of schistosome eggs with vascular endothelium. | the interaction of schistosome eggs with venular endothelium was studied using primary cultures of human umbilical vein endothelial cells. freshly oviposited and embryonated eggs of schistosoma mansoni, schistosoma japonicum, and schistosoma haematobium were used. the cultures were evaluated by light, scanning, and transmission electron microscopy. endothelial cell monolayers were found to retain eggs by actively migrating over those that came to lie on top of them. the monolayers then reestabli ... | 1995 | 7707199 |
| characterization of the recognition specificity of autoreactive, polyspecific monoclonal antibodies obtained from spleen cells of parasite-infected balb/c mice. | we examined the specificity of four monoclonal antibodies (moabs) obtained from schistosoma japonicum (1d, gao3), trypanosoma cruzi (tslo), and trichinella spiralis (tsy2) infected balb/c mice. all four moabs reacted not only with autologous parasite antigens but also with various heterologous parasite antigens and normal tissues. the antigen recognition pattern seen on western blots was almost identical in 1d and gao3, and in tslo and tsy2. furthermore, certain bands were identical among all fo ... | 1994 | 7708427 |
| passively transferable protection against schistosoma japonicum induced in the mouse by multiple vaccination with attenuated larvae: the development of immunity, antibody isotype responses and antigen recognition. | vaccination of mice with attenuated s. japonicum cercariae induces protection against secondary infection which can be transferred to naive mice with serum (vms). the presence of antibody does not per se impart protection as serum from mice carrying non-attenuated infections (cis), contains high levels of specific antibody, but confers no protection. here we describe the increased protection transferred (20 to 68%) with increased number of vaccinations (one to five) given to the donors, and its ... | 1994 | 7708432 |
| infection with schistosomes (schistosoma haematobium, schistosoma mansoni and schistosoma japonicum). | | 1994 | 7715071 |
| early treatment of schistosomal infection with artemether and praziquantel in rabbits. | artemether (art, beta-methyl ether of artemisinin) first synthesized by shanghai institute of materia medica, chinese academy of sciences, is effective against not only malaria but also schistosomiasis. when rabbits infected with schistosoma japonicum cercariae for 7 d were treated ig with art 10 mg.kg-1, the total worm reduction rates were 74.6-76.7%. if art (10 mg.kg-1) was given once weekly after the first treatment for 3-4 times, the total worm reduction rate was > 98%, and most of the rabbi ... | 1994 | 7717073 |
| early treatment with artemether and praziquantel in rabbits repeatedly infected with schistosoma japonicum cercariae. | when rabbits infected with 48-52 schistosoma japonicum cercariae once every wk for 6 times or every other day for 5 times were treated ig with artemether (art) 10 or 15 mg.kg-1 on d7 after the first infection, followed by the repeated administration of the same dose once every 1-2 wk, a promising effect was seen in the groups treated with art at higher dose. in another group of rabbits with the same drug administration regimens of praziquantel (pra) were also used in early treatment, but the ini ... | 1994 | 7720196 |
| [isolation and purification of glyceraldehyde-3-phosphate dehydrogenase from schistosoma japonicum]. | this paper recommends a simple and easy method to isolate and purify glyceraldehyde-3-phosphate dehydrogenase (gapdh) from schistosoma japonicum with electrophoretic purity. the recovery rate of 56.1% of glyceraldehyde-3-phosphate dehydrogenase from s. japonicum extract was achieved by ammonium sulfate precipitation, deae-cellulose column chromatography and sds-page. using sds-page, the presence of one major band in the final purified gapdh preparation was demonstrated. this protein had a mw of ... | 1994 | 7720198 |