| from structure and functions of steroidogenic enzymes to new technologies of gene engineering. | abstract (3/4) this review summarizes data about structural and functional organization of steroidogenic p450-dependent enzymatic systems. problems of catalysis of steroid substrate transformation, special features of mitochondrial type p450scc topogenesis, and abilities of some microbial electron transport proteins to support p450 activity in vitro and in vivo are considered. principal steps in the creation and catalytic properties of transgenic strains of escherichia coli, saccharomyces cerevi ... | 2009 | 20210705 |
| molecular cloning and characterization of a malic enzyme gene from the oleaginous yeast lipomyces starkeyi. | the malic enzyme-encoding cdna (gq372891) from the oleaginous yeast lipomyces starkeyi as 2.1560 was isolated, which has an 1719-bp open reading frame flanked by a 290-bp 5' untranslated sequence and a 92-bp 3' untranslated sequence. the proposed gene, lsme1, encoded a protein with 572 amino acid residues. the protein presented 58% sequence identity with the malic enzymes from yarrowia lipolytica clib122 and aspergillus fumigatus af293. the lsme1 gene was cloned into the vector pmal-p4x to expre ... | 2010 | 20217282 |
| cloning of exo-β-1,3-glucanase gene from a marine yeast williopsis saturnus and its overexpression in yarrowia lipolytica. | the exo-β-1,3-glucanase structural gene (wsexg1 gene, accession number: fj875997.2) was isolated from both the genomic dna and cdna of the marine yeast williopsis saturnus wc91-2 by inverse pcr and rt-pcr. an open reading frame of 1,254 bp encoding a 417 amino acid protein (isoelectric point: 4.5) with calculated molecular weight of 46.2 kda was characterized. the promoter of the gene (intronless) was located from -28 to -77 and had one tata box while its terminator contained the sequence aagaac ... | 2011 | 20336338 |
| surface display of acid protease on the cells of yarrowia lipolytica for milk clotting. | the acid protease structural gene was amplified from the genomic dna of saccharomycopsis fibuligera a11. when the gene was cloned into the multiple cloning site of the surface display vector pina1317-ylcwp110 and expressed in the cells of yarrowia lipolytica, the cells displaying the acid protease could form clear zone on the plate-containing milk indicating that they had extracellular acid protease activity. the cells displaying the acid protease can be used to effectively clot skimmed milk. th ... | 2010 | 20349181 |
| interaction of yeast mitochondria with fatty acids and mitochondria-targeted lipophilic cations. | the effect of fatty acids and mitochondria-targeted lipophilic cations (skq1, skq3, mitoq, and c(12)tpp) on tightly-coupled mitochondria from yeasts dipodascus (endomyces) magnusii and yarrowia lipolytica was investigated. micromolar concentrations of saturated and unsaturated fatty acids were found to decrease the membrane potential, which was recovered almost totally by atp and bsa. at low, micromolar concentrations, mitochondria-targeted lipophilic cations are "relatively weak, mild uncoupler ... | 2010 | 20367600 |
| factorial design to optimize biosurfactant production by yarrowia lipolytica. | in order to improve biosurfactant production by yarrowia lipolytica imufrj 50682, a factorial design was carried out. a 2(4) full factorial design was used to investigate the effects of nitrogen sources (urea, ammonium sulfate, yeast extract, and peptone) on maximum variation of surface tension (delta st) and emulsification index (ei). the best results (67.7% of ei and 20.9 mn m(-1) of delta st) were obtained in a medium composed of 10 g 1(-1) of ammonium sulfate and 0.5 g 1(-1) of yeast extract ... | 2010 | 20368788 |
| induction of permeability of the inner membrane of yeast mitochondria. | the current view on apoptosis is given, with a special emphasis placed on apoptosis in yeasts. induction of a nonspecific permeability transition pore (mptp) in mammalian and yeast mitochondria is described, particularly in mitochondria from yarrowia lipolytica and dipodascus (endomyces) magnusii yeasts, which are aerobes possessing the fully competent respiratory chain with all three points of energy conservation and well-structured mitochondria. they were examined for their ability to induce a ... | 2010 | 20370607 |
| citric acid production from glycerol-containing waste of biodiesel industry by yarrowia lipolytica in batch, repeated batch, and cell recycle regimes. | yarrowia lipolytica a-101-1.22 produces high citric acid (112 g l(-1)) with a yield of 0.6 g g(-1) and a productivity of 0.71 g l(-1) h(-1) during batch cultivation in the medium with glycerol-containing waste of biodiesel industry. however, it was observed that the specific citric acid production rate, which was maximal at the beginning of the biosynthesis, gradually decreases in the late production phase and it makes continuation of the process over 100 h pointless. the cell recycle and the re ... | 2010 | 20376633 |
| [protective and reactivating effect of the protein exometabolite on yeast cells inactivated by the ultraviolet irradiation]. | it has been shown that saccharomyces cerevisiae, kluyveromyces lactis, and candida utilis strains produce the protein exometabolites, which has a protective and reactivating effect on the ultraviolet irradiated yeast cells. the protective effect of the preliminary ultraviolet irradiated (activated) protein exometabolite of all strains increased 2-3 times, though its reactivating activity did not change. yarrowia lipolytica yeast cells, isolated from the areas with the high daily irradiation, and ... | 2010 | 20391763 |
| new insights into the effect of medium-chain-length lactones on yeast membranes. importance of the culture medium. | in hydrophobic compounds biotechnology, medium-chain-length metabolites often perturb cell activity. their effect is usually studied in model conditions of growth in glucose media. here, we study whether culture on lipids has an impact on the resistance of yarrowia lipolytica to such compounds: cells were cultured on glucose or oleate and submitted to gamma-dodecalactone. after a 60-min exposure to 3 g l(-1), about 80% of the glucose-grown cells (yeast extract peptone dextrose (ypd) cells) had l ... | 2010 | 20422183 |
| [susceptibility to fluconazole and voriconazole of candida species isolated from intensive care units patients in medellin, colombia (2001-2007)]. | disseminated candidiasis is caused by different candida species and mainly affects immunocompromised patients and those hospitalized in intensive care units (icu). | 2010 | 20450982 |
| isolation of lipase and citric acid producing yeasts from agro-industrial wastewater. | production of agro-industrial waste pollutants has become a major problem for many industries. however, agro-industrial wastes also can provide alternative substrates for industry and their utilization in this manner may help solve pollution problems. the aim of this study was to isolate yeasts from wastewater treatment plants that could be used to remove pollutants such as glycerol, paraffin and crude oil from the agro-industrial wastewater. in this study a total of 300 yeast isolates were obta ... | 2010 | 20450991 |
| inulin hydrolysis and citric acid production from inulin using the surface-engineered yarrowia lipolytica displaying inulinase. | the inu1 gene encoding exo-inulinase cloned from kluyveromyces marxianus cbs 6556 was ligated into the surface display plasmid and expressed in the cells of the marine-derived yeast yarrowia lipolytica which can produce citric acid. the expressed inulinase was immobilized on the yeast cells. the activity of the immobilized inulinase with 6 x his tag was found to be 22.6 u mg(-1) of cell dry weight after cell growth for 96 h. the optimal ph and temperature of the displayed inulinase were 4.5 and ... | 2010 | 20451653 |
| heterologous expression of thermobifida fusca thermostable alpha-amylase in yarrowia lipolytica and its application in boiling stable resistant sago starch preparation. | a gene encoding the thermostable alpha-amylase in thermobifida fusca ntu22 was amplified by pcr, sequenced, and cloned into yarrowia lipolytica p01g host strain using the vector pylsc1 allowing constitutive expression and secretion of the protein. recombinant expression resulted in high levels of extracellular amylase production, as high as 730 u/l in the hinton flask culture broth. it is higher than that observed in p. pastoris expression system and e. coli expression system. the purified amyla ... | 2010 | 20495998 |
| rationally engineered double substituted variants of yarrowia lipolytica lipase with enhanced activity coupled with highly inverted enantioselectivity towards 2-bromo phenyl acetic acid esters. | inverting enzyme enantioselectivity by protein engineering is still a great challenge. lip2p lipase from yarrowia lipolytica, which demonstrates a low s-enantioselectivity (e-value = 5) during the hydrolytic kinetic resolution of 2-bromo-phenyl acetic acid octyl esters (an important class of chemical intermediates in the pharmaceutical industry), was converted, by a rational engineering approach, into a totally r-selective enzyme (e-value > 200). this tremendous change in selectivity is the resu ... | 2010 | 20506522 |
| improving the lipid accumulation properties of the yeast cells for biodiesel production using molasses. | in this article the high lipid accumulation potential of candida lipolytica, candida tropicalis and rhodotorula mucilaginosa cells were shown in molasses medium. four different ph values were examined (4-7) to discover the optimum lipid accumulation medium. varied concentrations of (nh(4))(2)so(4) (0.5, 1.0, 1.5g/l) and molasses (6%, 8%, 10%) were tested to find the optimum carbon and nitrogen amounts for the highest cellular lipid production. it is shown that the maximum lipid content could be ... | 2010 | 20542422 |
| lack of the catalytic subunit of telomerase leads to growth defects accompanied by structural changes at the chromosomal ends in yarrowia lipolytica. | comparative analysis of the telomeres of distantly related species has proven to be helpful for identifying novel components involved in telomere maintenance. we therefore initiated such a study in the nonconventional yeast yarrowia lipolytica. its genome encodes only a small fraction of the proteins that are typically associated with telomeres in other yeast models, indicating that its telomeres may employ noncanonical means for their stabilization and maintenance. in this report, we have measu ... | 2010 | 20549213 |
| applying dimorphic yeasts as model organisms to study mycelial growth: part 2. use of mathematical simulations to identify different construction principles in yeast colonies. | the dimorphic yeasts candida boidinii and yarrowia lipolytica were applied as model organisms to study mycelial growth. a mathematical model of hybrid cellular automaton type was developed to analyze the impact of different biological assumptions on the predicted development of filamentous yeast colonies. the one-dimensional model described discrete cells and continuous distribution of nutrients. the simulation algorithm accounted for proliferation of cells, diffusion of nutrient, as well as bio ... | 2011 | 20549519 |
| applying dimorphic yeasts as model organisms to study mycelial growth: part 1. experimental investigation of the spatio-temporal development of filamentous yeast colonies. | colony development of the dimorphic yeasts yarrowia lipolytica and candida boidinii on solid agar substrates under glucose limitation served as a model system for mycelial development of higher filamentous fungi. strong differences were observed in the behaviour of both yeasts: c. boidinii colonies reached a final colony extension which was small compared to the size of the growth field. they formed cell-density profiles which steeply declined along the colony radius and no biomass decay process ... | 2011 | 20549520 |
| detection and analysis of alternative splicing in yarrowia lipolytica reveal structural constraints facilitating nonsense-mediated decay of intron-retaining transcripts. | hemiascomycetous yeasts have intron-poor genomes with very few cases of alternative splicing. most of the reported examples result from intron retention in saccharomyces cerevisiae and some have been shown to be functionally significant. here we used transcriptome-wide approaches to evaluate the mechanisms underlying the generation of alternative transcripts in yarrowia lipolytica, a yeast highly divergent from s. cerevisiae. | 2010 | 20573210 |
| [the study of adaptation mechanisms of yarrowia lipolytica yeast to alkaline conditions by means of proteomics]. | using proteomic technologies--two-dimensional electrophoresis in denaturing conditions in combination with mass spectroscopy of maldi-tof proteins--we demonstrated, for the first time, that the most noticeable alteration of protein composition of a yarrowia lipolytica cell during adaptation to alkaline conditions was an increase of mitochondrial proteins relatively to proteins of cytoplasm. | 2010 | 20586287 |
| preliminary safety assessment of yarrowia lipolytica extracellular lipase: results of acute and 28-day repeated dose oral toxicity studies in rats. | interest in extracellular lipase sourced from the non conventional yeast yarrowia lipolytica has increased over the last decade. the enzyme was recently suggested as a good candidate for pancreatic exocrine insufficiency treatment. however, there is still a lack of oral safety evaluation data. in this work, we conducted acute and 28-day repeated dose toxicity studies in rats. both male and female rats were first orally treated with fungal lipase at either single or repeated doses. the results de ... | 2010 | 20609427 |
| the subunit composition of mitochondrial nadh:ubiquinone oxidoreductase (complex i) from pichia pastoris. | respiratory complex i (nadh:quinone oxidoreductase) is an entry point to the electron transport chain in the mitochondria of many eukaryotes. it is a large, multisubunit enzyme with a hydrophilic domain in the matrix and a hydrophobic domain in the mitochondrial inner membrane. here we present a comprehensive analysis of the protein composition and post-translational modifications of complex i from pichia pastoris, using a combination of proteomic and bioinformatic approaches. forty-one subunits ... | 2010 | 20610779 |
| production of succinic acid at low ph by a recombinant strain of the aerobic yeast yarrowia lipolytica. | biotechnological production of weak organic acids such as succinic acid is most economically advantageous when carried out at low ph. among naturally occurring microorganisms, several bacterial strains are known to produce considerable amounts of succinic acid under anaerobic conditions but they are inefficient in performing the low-ph fermentation due to their physiological properties. we have proposed therefore a new strategy for construction of an aerobic eukaryotic producer on the basis of t ... | 2010 | 20632369 |
| screening of a thiamine-auxotrophic yeast for alpha-ketoglutaric acid overproduction. | to obtain a thiamine-auxotrophic yeast strain that overproduces alpha-ketoglutaric acid (alpha-kg) from glycerol and to investigate nutrient effects on alpha-kg production. | 2010 | 20636327 |
| surface display of active lipases lip7 and lip8 from yarrowia lipolytica on saccharomyces cerevisiae. | lipase has been used widely in industry. in this study, we have constructed two recombinant saccharomyces cerevisiae strains displaying two active lipases on the cell surface by cell surface engineering. the genes encoding yarrowia lipolytica lipases lip7 and lip8 were fused with the gene encoding small binding subunit aga2 of a-agglutinin. localization of the lip7 and lip8 on the cell surface was confirmed by immunofluorescence microscopy. besides, the putative signal sequences of lip7 and lip8 ... | 2010 | 20676630 |
| [expression of phytase gene phya in yarrowia lipolytica po1h]. | using the polymmerse chain reaction (pcr), we amplified the phytase gene phya from pichia pastoris gs115-phya in aspergillus niger nrrl3135 without the signal peptide sequence and intron sequence,. then, it was cloned into pina1297 vector to generate a recombinant vector of pina1297-phya. pina1297-phya was linearized and transformed into yarrowia lipolytica po1h by the lithium acetate method. the positive transformants were obtained by ynb(casa) and ppb plates, after induced in ym medium at 28 d ... | 2010 | 20684304 |
| chromosomal g + c content evolution in yeasts: systematic interspecies differences, and gc-poor troughs at centromeres. | the g + c content at synonymous codon positions (gc3s) in genes varies along chromosomes in most eukaryotes. in saccharomyces cerevisiae, regions of high gc3s are correlated with recombination hot spots, probably due to biased gene conversion. here we examined how gc3s differs among groups of related yeast species in the saccharomyces and candida clades. the chromosomal locations of gc3s peaks and troughs are conserved among four saccharomyces species, but we find that there have been highly con ... | 2010 | 20693156 |
| replacing synthetic with microbial surfactants as collectors in the treatment of aqueous effluent produced by acid mine drainage, using the dissolved air flotation technique. | dissolved air flotation (daf) is a well-established separation process employing micro bubbles as a carrier phase. the application of this technique in the treatment of acid mine drainage, using three yeast biosurfactants as alternative collectors, is hereby analyzed. batch studies were carried out in a 50-cm high acrylic column with an external diameter of 2.5 cm. high percentages (above 94%) of heavy metals fe(iii) and mn(ii) were removed by the biosurfactants isolated from candida lipolytica ... | 2011 | 20714828 |
| the human ku autoantigen shares amino acid sequence homology with fungal, but not bacterial and viral, proteins. | context: molecular mimicry between autoantigens and microbial antigens is a possible triggering mechanism of autoimmunity. human ku is a dna-associated autoantigen targeted by autoantibodies in patients with systemic lupus erythematosus and related disorders; available data are consistent with a role of molecular mimicry in the pathogenesis of anti-ku autoimmunity, but no research exist on this topic. objective: we aimed to define the most probable microbial triggers of anti-ku autoimmunity via ... | 2011 | 20718578 |
| stacking multiple transgenes at a selected genomic site via repeated recombinase-mediated dna cassette exchanges. | recombinase-mediated dna cassette exchange (rmce) has been successfully used to insert transgenes at previously characterized genomic sites in plants. following the same strategy, groups of transgenes can be stacked to the same site through multiple rounds of rmce. a gene-silencing cassette, designed to simultaneously silence soybean (glycine max) genes fatty acid ω-6 desaturase 2 (fad2) and acyl-acyl carrier protein thioesterase 2 (fatb) to improve oleic acid content, was first inserted by rmce ... | 2010 | 20720171 |
| roles of multiple acyl-coa oxidases in the routing of carbon flow towards β-oxidation and polyhydroxyalkanoate biosynthesis in yarrowia lipolytica. | the oleaginous yeast yarrowia lipolytica possesses six acyl-coa oxidase (aox) isoenzymes encoded by genes pox1-pox6. the respective roles of these multiple aox isoenzymes were studied in recombinant y. lipolytica strains that express heterologous polyhydroxyalkanoate (pha) synthase (phac) of pseudomonas aeruginosa in varying pox genetic backgrounds, thus allowing assessment of the impact of specific aox enzymes on the routing of carbon flow to β-oxidation or to pha biosynthesis. analysis of pha ... | 2010 | 20726896 |
| safety assessment of epa-rich oil produced from yeast: results of a 90-day subchronic toxicity study. | the safety of eicosapentaenoic acid (epa) oil produced from genetically modified yarrowia lipolytica yeast was evaluated following 90 days of exposure. groups of rats received 0 (olive oil), 98, 488, or 976 mg epa/kg/day, or gras fish oil or deionized water by oral gavage. rats were evaluated for in-life, neurobehavioral, anatomic and clinical pathology parameters. lower serum cholesterol (total and non-hdl) was observed in medium and high epa and fish oil groups. lower hdl was observed in high ... | 2010 | 20804805 |
| [gene cloning, codon optimization and functional expression of yarrawia lipolytica lipase lip1]. | to implement inducible and constitutive over-expression of yarrowia lipolytica lipase gene lipl in pichia pastoris using codon optimization. | 2010 | 20815247 |
| a molecular approach to optimize hifn α2b expression and secretion in yarrowia lipolytica. | in this work, we investigated the effect of codon bias and consensus sequence (caca) at the translation initiation site on the expression level of heterologous proteins in yarrowia lipolytica; human interferon alpha 2b (hifn-α2b) was studied as an example. a codon optimized hifn-α2b gene was synthesized according to the frequency of codon usage in y. lipolytica. both wild-type (ifn-wt) and optimized hifn-α2b (ifn-op) genes were expressed under the control of a strong inducible promoter acyl-co-e ... | 2011 | 20821205 |
| combined ph and high hydrostatic pressure effects on lactococcus starter cultures and candida spoilage yeasts in a fermented milk test system during cold storage. | the combined effects of high pressure processing (hpp) and ph on the glycolytic and proteolytic activities of lactococcus lactis subsp. lactis, a commonly used cheese starter culture and the outgrowth of spoilage yeasts of candida species were investigated in a fermented milk test system. to prepare the test system, l. lactis subsp. lactis c10 was grown in uht skim milk to a final ph of 4.30 and then additional samples for treatment were prepared by dilution of fermented milk with uht skim milk ... | 2010 | 20832684 |
| safety assessment of epa-rich triglyceride oil produced from yeast: genotoxicity and 28-day oral toxicity in rats. | the 28-day repeat-dose oral and genetic toxicity of eicosapentaenoic acid triglyceride oil (epa oil) produced from genetically modified yarrowia lipolytica yeast were assessed. groups of rats received 0 (olive oil), 940, 1880, or 2820 mg epa oil/kg/day, or fish oil (sardine/anchovy source) by oral gavage. lower total serum cholesterol was seen in all epa and fish oil groups. liver weights were increased in the medium and high-dose epa (male only), and fish oil groups but were considered non-adve ... | 2010 | 20868718 |
| expression of inulinase gene in the oleaginous yeast yarrowia lipolytica and single cell oil production from inulin-containing materials. | yarrowia lipolytica aca-dc 50109 has been reported to be an oleaginous yeast and significant quantities of lipids were accumulated inside the yeast cells. in this study, the inu1 gene encoding exo-inulinase cloned from kluyveromyces marxianus cbs 6556 was ligated into the expression plasmid pina1317 and expressed in the cells of the oleaginous yeast. the activity of the inulinase with 6 × his tag secreted by the transformant z31 obtained was found to be 41.7u ml(-1) after cell growth for 78 h. a ... | 2010 | 20883812 |
| occurrence and dominance of yeast species in naturally fermented milk from the tibetan plateau of china. | to determine which yeasts are present in the naturally fermented milks of china, 69 samples made by the nomads of tibet were collected from the tibetan plateau in china. from these samples, 225 strains of yeast were isolated and identified using conventional microbiological analysis and gene sequencing analysis of the d1/d2 domain of the large subunit (26s) ribosomal dna. the results showed that the total concentration of yeasts in these samples ranged from 5.01 to 8.97 log10 colony-forming unit ... | 2010 | 20921981 |
| exploring the conformational states and rearrangements of yarrowia lipolytica lipase. | we report the 1.7 å resolution crystal structure of the lip2 lipase from yarrowia lipolytica in its closed conformation. the lip2 structure is highly homologous to known structures of the fungal lipase family (thermomyces lanuginosa, rhizopus niveus, and rhizomucor miehei lipases). however, it also presents some unique features that are described and discussed here in detail. structural differences, in particular in the conformation adopted by the so-called lid subdomain, suggest that the openin ... | 2010 | 20923657 |
| tay1 protein, a novel telomere binding factor from yarrowia lipolytica. | inspection of the complete genome of the yeast yarrowia lipolytica for the presence of genes encoding homologues of known telomere-binding proteins surprisingly revealed no counterparts of typical yeast myb domain-containing telomeric factors including rap1 or taz1. instead, we identified a gene, yaliod10923g, encoding a protein containing two myb domains, exhibiting a high degree of similarity to the myb domain of human telomeric proteins trf1 and trf2 and homologous to an essential fission yea ... | 2010 | 20923774 |
| folding proteome of yarrowia lipolytica targeting with uracil permease mutants. | the acquisition of the correct folding of membrane proteins is a crucial process that involves several steps from the recognition of nascent protein, its targeting to the endoplasmic reticulum membrane, its insertion, and its sorting to its final destination. yarrowia lipolytica is a hemiascomycetous dimorphic yeast and an alternative eukaryotic yeast model with an efficient secretion pathway. to better understand the quality control of membrane proteins, we constructed a model system based on t ... | 2010 | 20949976 |
| a highly stable yarrowia lipolytica lipase formulation for the treatment of pancreatic exocrine insufficiency. | yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. nevertheless, no systematic studies on its stability under physiological conditions pertaining to the human gi (gastrointestinal) tract have been published. stability of various y. lipolytica lipase powder formulations at various physiological ph values as well as the effect of digestive proteases and bile salts on enzyme activity were investigated. ... | 2010 | 20958263 |
| an ortholog of fara of aspergillus nidulans is implicated in the transcriptional activation of genes involved in fatty acid utilization in the yeast yarrowia lipolytica. | the yeast yarrowia lipolytica effectively utilizes hydrophobic substrates such as fatty acids and n-alkanes. to identify a gene(s) regulating fatty acid utilization in y. lipolytica, we first studied homologous genes to oaf1 and pip2 of saccharomyces cerevisiae, but their disruption did not change growth on oleic acid at all. we next characterized a y. lipolytica gene, por1 (primary oleate regulator 1), an ortholog of fara encoding a transcriptional activator that regulates fatty acid utilizatio ... | 2010 | 20977884 |
| overexpression of alpha-ketoglutarate dehydrogenase in yarrowia lipolytica and its effect on production of organic acids. | the yeast yarrowia lipolytica is one of the most intensively studied "non-conventional" yeast species. its ability to secrete various organic acids, like pyruvic (pa), citric, isocitric, and alpha-ketoglutaric (kga) acid, in large amounts is of interest for biotechnological applications. we have studied the effect of the alpha-ketoglutarate dehydrogenase (kgdh) complex on the production process of kga. being well studied in saccharomyces cerevisiae this enzyme complex consists of three subunits: ... | 2010 | 21057948 |
| quantitative study of lipase secretion, extracellular lipolysis, and lipid storage in the yeast yarrowia lipolytica grown in the presence of olive oil: analogies with lipolysis in humans. | lipase secretion, extracellular lipolysis, and fatty acid uptake were quantified in the yeast yarrowia lipolytica grown in the presence of olive oil and/or glucose. specific lipase assays, western blot analysis, and elisa indicated that most of the lipase activity measured in y. lipolytica cultures resulted from the yllip2 lipase. lipase production was triggered by olive oil and, during the first hours of culture, most of the lipase activity and yllip2 immunodetection remained associated with th ... | 2010 | 21076918 |
| cross-species induction of antimicrobial compounds, biosurfactants and quorum-sensing inhibitors in tropical marine epibiotic bacteria by pathogens and biofouling microorganisms. | enhancement or induction of antimicrobial, biosurfactant, and quorum-sensing inhibition property in marine bacteria due to cross-species and cross-genera interactions was investigated. four marine epibiotic bacteria (bacillus sp. s3, b. pumilus s8, b. licheniformis d1, and serratia marcescens v1) displaying antimicrobial activity against pathogenic or biofouling fungi (candida albicans ca and yarrowia lipolytica yl), and bacteria (pseudomonas aeruginosa pa and bacillus pumilus bp) were chosen fo ... | 2010 | 21086131 |
| construction of high sensitive detection system for endocrine disruptors with yeast n-alkane-assimilating yarrowia lipolytica. | to construct a highly sensitive detection system for endocrine disruptors, we have compared the activity of promoters with the alk1, icl1, rps7 and tef1 for heterologous gene in yarrowia lipolytica. the promoters were introduced into the upstream of lacz or heralpha reporter gene, respectively, and the activity was evaluated by beta-galactosidase assay by lacz or western blot analysis by heralpha. the expression analysis revealed that the alk1 and icl1 promoter were induced by n-decane and by et ... | 2010 | 21124063 |
| microorganisms form exocellular structures, trophosomes, to facilitate biodegradation of oil in aqueous media. | cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. among the microorganisms studied here, the yeasts (schwanniomyces occidentalis, torulopsis candida, candida tropicalis, candida lipolytica, candida maltosa, candida paralipolytica) and two representative bacteria (rhodococcus sp. and pseudomonas putida) produced exocellular structures compos ... | 2010 | 21182540 |
| essential role of ylmpo1, a novel yarrowia lipolytica homologue of saccharomyces cerevisiae mnn4, in mannosylphosphorylation of n- and o-linked glycans. | mannosylphosphorylation of n- and o-glycans, which confers negative charges on the surfaces of cells, requires the functions of both mnn4 and mnn6 in saccharomyces cerevisiae. to identify genes relevant to mannosylphosphorylation in the dimorphic yeast yarrowia lipolytica, the molecular functions of five y. lipolytica genes showing significant sequence homology with s. cerevisiae mnn4 and mnn6 were investigated. a set of mutant strains in which y. lipolytica mnn4 and mnn6 homologues were deleted ... | 2010 | 21183647 |
| intracellular characterization of aerobic glucose metabolism in seven yeast species by 13c flux analysis and metabolomics. | key distinguishing characteristics of yeast glucose metabolism are the relative proportions of fermentation and respiration. crabtree-positive yeast species exhibit a respirofermentative metabolism, whereas aerobic species respire fully without secretion of fermentation byproducts. physiological data suggest a gradual transition in different species between these two states. here, we investigate whether this gradual transition also occurs at the intracellular level by quantifying the intracellul ... | 2011 | 21205161 |
| isolation, characterization and expression analysis of the ornithine decarboxylase gene (odc1) of the entomopathogenic fungus, metarhizium anisopliae. | the gene odc1, which codes for the ornithine decarboxylase enzyme, was isolated from the entomopathogenic fungus, metarhizium anisopliae. the deduced amino acid sequence predicted a protein of 447 amino acids with a molecular weight of 49.3kda that contained the canonical motifs of ornithine decarboxylases. the odc1 cdna sequence was expressed in escherichia coli cells; radiometric enzyme assays showed that the purified recombinant protein had ornithine decarboxylase activity. the optimum ph of ... | 2011 | 21236653 |
| process analysis of variables for standardization of antifungal susceptibility testing of nonfermentative yeasts. | nonfermentative yeasts, such as cryptococcus spp., have emerged as fungal pathogens during the last few years. however, standard methods to measure their antifungal susceptibility (antifungal susceptibility testing [ast]) are not completely reliable due to the impaired growth of these yeasts in standard media. in this work, we have compared the growth kinetics and the antifungal susceptibilities of representative species of nonfermentative yeasts such as cryptococcus neoformans, cryptococcus gat ... | 2011 | 21245438 |
| antimicrobial and anti-adhesive potential of a biosurfactant rufisan produced by candida lipolytica ucp 0988. | in the last years, researches developed with biosurfactants for application in the medical area have been revealing the promising biological activities of these biomolecules. in this work the antimicrobial and anti-adhesive properties of a biosurfactant rufisan isolated from the yeast candida lipolytica ucp 0988, growth in a medium supplemented with ground nut refinery residue was determined against several microorganisms. the biosurfactant was able to reduce the water surface tension from 70 to ... | 2010 | 21247740 |
| the 'lipoyeasts' project: using the oleaginous yeast yarrowia lipolytica in combination with specific bacterial genes for the bioconversion of lipids, fats and oils into high-value products. | the oleochemical industry is currently still dominated by conventional chemistry, with biotechnology only starting to play a more prominent role, primarily with respect to the biosurfactants or lipases, e.g. as detergents, or for biofuel production. a major bottleneck for all further biotechnological applications is the problem of the initial mobilization of cheap and vastly available lipid and oil substrates, which are then to be transformed into high-value biotechnological, nutritional or phar ... | 2011 | 21255371 |
| chemostat study of citric acid production from glycerol by yarrowia lipolytica. | the aim of the study was to examine how the dilution rate and the chemical composition of the production medium impacts on the synthesis of citric acid by the yarrowia lipolytica strain wratislavia awg7 from glycerol in a chemostat culture. the yeast y. lipolytica wratislavia awg7, an acetate (acet(-)) and morphological (fil(-)) mutant, was cultured in a nitrogen- and phosphorus-limited medium at the dilution rate of 0.009-0.031h(-1) in the chemostat. under steady-state conditions, the increase ... | 2011 | 21262285 |
| distribution of introns in fungal histone genes. | saccharomycotina and taphrinomycotina lack intron in their histone genes, except for an intron in one of histone h4 genes of yarrowia lipolytica. on the other hand, basidiomycota and perizomycotina have introns in their histone genes. we compared the distributions of 81, 47, 79, and 98 introns in the fungal histone h2a, h2b, h3, and h4 genes, respectively. based on the multiple alignments of the amino acid sequences of histones, we identified 19, 13, 31, and 22 intron insertion sites in the hist ... | 2011 | 21304581 |
| high-level production of extracellular lipase by yarrowia lipolytica mutants from methyl oleate. | the yeast yarrowia lipolytica degrades efficiently low-cost hydrophobic substrates for the production of various added-value products such as lipases. to obtain yeast strains producing high levels of extracellular lipase, y. lipolytica dsm3286 was subjected to mutation using ethyl methanesulfonate (ems) and ultraviolet (uv) light. twenty mutants were selected out of 1600 mutants of y. lipolytica treated with ems and uv based on lipase production ability on selective medium. a new industrial medi ... | 2011 | 21324386 |
| c- and n-catabolic utilization of tricarboxylic acid cycle-related amino acids by scheffersomyces stipitis and other yeasts. | scheffersomyces stipitis and the closely related yeast candida shehatae assimilated the l-amino acids glutamate, aspartate and proline as both carbon and nitrogen sole sources. we also found this rarely investigated ability in ascomycetous species such as candida glabrata, c. reukaufii, c. utilis, debaryomyces hansenii, kluyveromyces lactis, k. marxianus, candida albicans, l. elongisporus, meyerozyma guilliermondii, c. maltosa, pichia capsulata and yarrowia lipolytica and in basidiomycetous spec ... | 2011 | 21360752 |
| two-step synthesis of fatty acid ethyl ester from soybean oil catalyzed by yarrowia lipolytica lipase. | | 2011 | 21366905 |
| characterization of the regulatory subunit of yarrowia lipolytica camp-dependent protein kinase. evidences of a monomeric protein. | camp-dependent protein kinase (pka) catalytic (c) and regulatory (r) subunits from yarrowia lipolytica are encoded by single genes, tpk1 and rka1, respectively. here we performed the heterologous expression, purification and characterization of the r subunit from y. lipolytica yeast cells, and explored the main biochemical features of the pka. the purified recombinant r, active and capable to interact with c subunit was used to prepare highly specific polyclonal antiserum. sucrose-gradient centr ... | 2011 | 21382337 |
| fungemia caused by yarrowia lipolytica in a patient with acute lymphoblastic leukemia. | yarrowia lipolytica is a weakly pathogenic yeast that is rarely isolated from the blood. we treated a patient with acute lymphoblastic leukemia who underwent catheter-related fungemia attributable to this organism. the patient was recovered after 3 day's prophylactic dose of fluconazole and removal of deep venous catheter. yarrowia lipolytica was also identified from yeast extract capsule that was administered to this child. this case indicated that it is necessary to give antifungal therapy and ... | 2011 | 21383637 |
| a high-definition native polyacrylamide gel electrophoresis system for the analysis of membrane complexes. | native polyacrylamide gel electrophoresis (page) is an important technique for the analysis of membrane protein complexes. a major breakthrough was the development of blue native (bn-) and high resolution clear native (hrcn-) page techniques. although these techniques are very powerful, they could not be applied to all systems with the same resolution. we have developed an alternative protocol for the analysis of membrane protein complexes of plant chloroplasts and cyanobacteria, which we termed ... | 2011 | 21418111 |
| functional survey for heterologous sugar transport proteins, using saccharomyces cerevisiae as a host. | molecular transport is a key process in cellular metabolism. this step is often limiting when using a nonnative carbon source, as exemplified by xylose catabolism in saccharomyces cerevisiae. as a step toward addressing this limitation, this study seeks to characterize monosaccharide transport preference and efficiency. a group of 26 known and putative monosaccharide transport proteins was expressed in a recombinant saccharomyces cerevisiae host unable to transport several monosaccharides. a gro ... | 2011 | 21421781 |
| oil-in-water emulsions characterization by laser granulometry and impact on ?-decalactone production in yarrowia lipolytica. | oil-in-water emulsions composed of methyl ricinoleate (mr) or castor oil (co) as the organic phase, stabilized by tween 80, are in the basis of the biotechnological production of ?-decalactone. yarrowia lipolytica was used due to its ability to grow on hydrophobic substrates and to carry out the biotransformation. the characterization of oil droplets size distribution by laser granulometry was performed under different oil concentrations. the impact of the presence of cells on droplets size was ... | 2011 | 21431848 |
| variability of the lipolytic activity in yarrowia lipolytica strains in pork fat. | this work studied the variability in lipolytic activity in 35 strains of yarrowia lipolytica inoculated in pork fat after 7 and 21 days of storage at 15 °c. the strains were able to generate three different hydrolysis profiles. in particular, the strains po10, po14, ro1, ro5, y15, y16a, y20, b5, 7b, 7b3, 16b and 21c caused an increase with time in concentrations of c16:0, c18:0, c18:1, c18:1(?11) and c18:2 which were the predominant free fatty acids (ffas). on the contrary, the strains po1, po19 ... | 2011 | 21454020 |
| repeated fed-batch fermentation using biosensor online control for citric acid production by yarrowia lipolytica. | biosensor-controlled substrate feeding was used in a citric acid production process with the yeast strain yarrowia lipolytica h222 with glucose as the carbon source. the application of an online glucose biosensor measurement facilitated the performance of long-time repeated fed-batch process with automated bioprocess control. ten cycles of repeated fed-batch fermentation were carried out in order to validate both the stability of the microorganism for citric acid production and the robustness of ... | 2011 | 21458506 |
| oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid. | this paper explores the use of the hydrolysate from the dilute sulfuric acid pretreatment of wheat straw for microbial oil production. the resulting hydrolysate was composed of pentoses (24.3g/l) and hexoses (4.9 g/l), along with some other degradation products, such as acetic acid, furfural, and hydroxymethylfurfural (hmf). five oleaginous yeast strains, cryptococcus curvatus, rhodotorula glutinis, rhodosporidium toruloides, lipomyces starkeyi, and yarrowia lipolytica, were evaluated by using t ... | 2011 | 21463940 |
| oily yeasts as oleaginous cell factories. | oily yeasts have been described to be able to accumulate lipids up to 20% of their cellular dry weight. these yeasts represent a minor proportion of the total yeast population, and only 5% of them have been reported as able to accumulate more than 25% of lipids. the oily yeast genera include yarrowia, candida, rhodotorula, rhodosporidium, cryptococcus, trichosporon, and lipomyces. more specifically, examples of oleaginous yeasts include the species: lipomyces starkeyi, rhodosporidium toruloides, ... | 2011 | 21465305 |
| evaluation of pathogenic fungi occurrence in traumatogenic structures of freshwater fish. | fungal infections in human skin, such as sporotrichosis, can occur after fish induced trauma. this work aimed to identify fungi in freshwater fish that are pathogenic to humans. | 2011 | 21468475 |
| impacts of cell surface characteristics on population dynamics in a sequencing batch yeast reactor treating vegetable oil-containing wastewater. | ten yeast strains acquired from different sources and capable of utilizing vegetable oil or related compounds (fatty acid or oleic acid) as sole carbon sources were inoculated into a sequencing batch reactor (sbr) for the treatment of high-strength vegetable oil-containing wastewater. the sbr system stably removed >89% of chemical oxygen demand (cod) and >99% of oil when fed with wastewater containing 15 g/l cod and 10 g/l oil in average. denaturing gradient gel electrophoresis of polymerase cha ... | 2011 | 21468715 |
| efficient cell surface display of lip2 lipase using c-domains of glycosylphosphatidylinositol-anchored cell wall proteins of yarrowia lipolytica. | the cell surface display of enzymes is of great interest because of its simplified purification stage and the possibility for recycling in industrial processes. in this study, we have focused on the cell wall immobilization of yarrowia lipolytica lip2 protein-an enzyme that has a wide technological application. by genome analysis of y. lipolytica in addition to already characterized ylcwp1, we identified five putative open reading frames encoding glycosylphosphatidylinositol-anchored proteins. l ... | 2011 | 21494864 |
| variability of the lipolytic activity and volatile molecules production by a strain of yarrowia lipolytica in pork fat and its dependence on environmental conditions. | the effects of selected variables, i.e. temperature, water activity and yeast inoculation level, on the lipolytic pattern and volatile production by yarrowia lipolytica y16a (chosen on the basis of a previous screening) were assessed. the variables were varied according to a central composite design and the models obtained enabled evaluation and weighting of the effects of the independent variables on the free fatty acids (ffas) and volatile profiles in pork fat based medium. the polynomial mode ... | 2011 | 21497447 |
| identical domains of yarrowia lipolytica vps23 are required for both escrt and rim pathways, but the latter needs an interaction between the vps23 uev domain and rim8/palf. | a conserved pathway, called rim or pal, transduces the ambient ph signal in ascomycetous yeasts and fungi, respectively. this pathway requires most of the components of the endosomal sorting complex required for transport (escrt) pathway. in the yeast yarrowia lipolytica, a functional analysis of the escrt-i subunit vps23 was carried out by in-frame deletions of each of the conserved domains to test whether vps23 functions in the rim and escrt pathways could be separated. these two pathways were ... | 2011 | 21539706 |
| a scaffold of accessory subunits links the peripheral arm and the distal proton pumping module of mitochondrial complex i. | mitochondrial nadh:ubiquinone oxidoreductase (complex i) is a very large membrane protein complex with a central function in energy metabolism. complex i from the aerobic yeast yarrowia lipolytica comprises 14 central subunits that harbour the bioenergetic core functions and at least 28 accessory subunits. despite progress in structure determination the position of individual accessory subunits in the enzyme complex remains largely unknown. proteomic analysis of subcomplex i-delta revealed that ... | 2011 | 21545356 |
| simultaneous production of citric acid and invertase by yarrowia lipolytica suc(+) transformants. | simultaneous production of citric acid (ca) and invertase by yarrowia lipolytica a-101-b56-5 (suc(+) clone) growing from sucrose, mixture of glucose and fructose, glucose or glycerol was investigated. among the tested substrates the highest concentration of ca was reached from glycerol (57.15g/l) with high yield (y(ca/s)=0.6g/g). when sucrose was used, comparable amount of ca was secreted (45g/l) with slightly higher yield (y(ca/s)=0.643g/g). in all cultures amount of isocitrate (ica) was below ... | 2011 | 21549590 |
| the lipases from yarrowia lipolytica: genetics, production, regulation, biochemical characterization and biotechnological applications. | lipases are serine hydrolases that catalyze in nature the hydrolysis of ester bonds of long chain triacylglycerol into fatty acid and glycerol. however, in favorable thermodynamic conditions, they are also able to catalyze reactions of synthesis such as esterification or amidation. the non-conventional yeast yarrowia lipolytica possesses 16 paralogs of genes coding for lipase. however, little information on all those paralogs has been yet obtained and only three isoenzymes, namely lip2p, lip7p a ... | 2011 | 21550394 |
| physiological uncoupling of mitochondrial oxidative phosphorylation. studies in different yeast species. | under non-phosphorylating conditions a high proton transmembrane gradient inhibits the rate of oxygen consumption mediated by the mitochondrial respiratory chain (state iv). slow electron transit leads to production of reactive oxygen species (ros) capable of participating in deleterious side reactions. in order to avoid overproducing ros, mitochondria maintain a high rate of o(2) consumption by activating different exquisitely controlled uncoupling pathways. different yeast species possess one ... | 2011 | 21556887 |
| control of glucosylceramide production and morphogenesis by the bar1 ceramide synthase in fusarium graminearum. | the contribution of plasma membrane proteins to the virulence of plant pathogenic fungi is poorly understood. accordingly, the objective of this study was to characterize the acyl-coa dependent ceramide synthase bar1 (previously implicated in plasma membrane organization) in the wheat pathogen fusarium graminearum. the role of bar1 in mediating cell membrane organization was confirmed as δbar1 mutants failed to display a distinct sterol-rich domain at the hyphal tip. the δbar1 mutants were non-p ... | 2011 | 21559419 |
| lipid production by culturing oleaginous yeast and algae with food waste and municipal wastewater in an integrated process. | food waste and municipal wastewater are promising feedstocks for microbial lipid biofuel production, and corresponding production process is to be developed. in this study, different oleaginous yeast strains were tested to grow in hydrolyzed food waste, and growths of cryptococcus curvatus, yarrowia lipolytica, and rhodotorula glutinis in this condition were at same level as in glucose culture as control. these strains were further tested to grow in municipal primary wastewater. c. curvatus and ... | 2011 | 21567213 |
| design of an efficient medium for heterologous protein production in yarrowia lipolytica: case of human interferon alpha 2b. | abstract: background: the non conventional yeast yarrowia lipolytica has aroused a strong industrial interest for heterologous protein production. however most of the studies describing recombinant protein production by this yeast rely on the use of complex media, such media are not convenient for large scale production particularly for products intended for pharmaceutical applications. in addition medium composition can also affect the production yield. hence it is necessary to design an effici ... | 2011 | 21595994 |
| engineering polyhydroxyalkanoate content and monomer composition in the oleaginous yeast yarrowia lipolytica by modifying the ß-oxidation multifunctional protein. | recombinant strains of the oleaginous yeast yarrowia lipolytica expressing the pha synthase gene (phac) from pseudomonas aeruginosa in the peroxisome were found able to produce polyhydroxyalkanoates (pha). pha production yield, but not the monomer composition, was dependent on pox genotype (pox genes encoding acyl-coa oxidases) (haddouche et al. fems yeast res 10:917-927, 2010). in this study of variants of the y. lipolytica β-oxidation multifunctional enzyme, with deletions or inactivations of ... | 2011 | 21603933 |
| involvement of the g3p shuttle and β-oxidation pathway in the control of tag synthesis and lipid accumulation in yarrowia lipolytica. | the oleaginous yeast yarrowia lipolytica can accumulate up to 38% of its dry weight (dw) as lipids. factors involved in lipid accumulation, particularly triglycerides, are not well identified. using different mutations in the glycerol-3-phosphate (g3p) shuttle pathway (δgut2 affecting the anabolic dehydrogenase or overexpressing gpd1 affecting the catabolic dehydrogenase), we were able to modulate g3p concentration. we show that in a po1d genetic background, gpd1 overexpression, gut2 inactivatio ... | 2011 | 21620992 |
| allosteric nucleotide-binding site in the mitochondrial nadh:ubiquinone oxidoreductase (respiratory complex i). | the rotenone-insensitive nadh:hexaammineruthenium iii (har) oxidoreductase reactions catalyzed by bovine heart and yarrowia lipolytica submitochondrial particles or purified bovine complex i are stimulated by atp and other purine nucleotides. the soluble fraction of mammalian complex i (fp) and prokaryotic complex i homolog ndh-1 in paracoccus denitrificans plasma membrane lack stimulation of their activities by atp. the stimulation appears as a decrease in apparent k(m) values for nadh and har. ... | 2011 | 21624365 |
| successful treatment of central venous catheter infection due to candida lipolytica by caspofungin-lock therapy. | | 2011 | 21672041 |
| identification and characterization of dga2, an acyltransferase of the dgat1 acyl-coa:diacylglycerol acyltransferase family in the oleaginous yeast yarrowia lipolytica. new insights into the storage lipid metabolism of oleaginous yeasts. | triacylglycerols (tag) and steryl esters (se) are the principal storage lipids in all eukaryotic cells. in yeasts, these storage lipids accumulate within special organelles known as lipid bodies (lb). in the lipid accumulation-oriented metabolism of the oleaginous yeast yarrowia lipolytica, storage lipids are mostly found in the form of tag, and only small amounts of se accumulate. we report here the identification of a new dag acyltransferase gene, dga2, homologous to the are genes of saccharom ... | 2011 | 21808970 |
| cloning, expression and characterization of a new lipase from yarrowia lipolytica. | bioinformatic analysis of the yarrowia lipolytica clib122 genome has revealed 18 putative lipase genes all of which were expressed in escherichia coli and screened for hydrolyzing activities against p-nitrophenyl-palmitate. one positive transformant containing an orf of 1,098 bp encoding a protein of 365 amino acids was obtained. to characterize its enzymatic properties, the lipase gene was functionally expressed in pichia pastoris. the resulting lipase exhibited the highest activity towards p-n ... | 2011 | 21805187 |
| yali0e32769g (dga1) and yali0e16797g (lro1) encode major triacylglycerol synthases of the oleaginous yeast yarrowia lipolytica. | the oleaginous yeast yarrowia lipolytica has an outstanding capacity to produce and store triacylglycerols resembling adipocytes of higher eukaryotes. here, the identification of two genes yali0e32769g (dga1) and yali0e16797g (lro1) encoding major triacylglycerol synthases of yarrowia lipolytica is reported. heterologous expression of either dga1 or lro1 in a mutant of the budding yeast saccharomyces cerevisiae defective in triacylglycerol synthesis restores the formation of this neutral lipid. ... | 2011 | 21782973 |
| studies on the lipolytic activity of sonicated enzymes from yarrowia lipolytica. | the aim of this study was to evaluate the efficiency of sonication in releasing protein from a widespread lipase-producing yeast, yarrowia lipolytica kkp 379, and to examine the impact of ultrasound waves generated in a horn-type sonicator on the lipolytic activity of y. lipolytica in the hydrolysis of p-nitrophenyl laurate. in this paper, we focused on a few parameters of ultrasound cell disruption, such as the time of sonication, acoustic power, storage time of the frozen yeast biomass used in ... | 2011 | 21778102 |
| enhanced production of citric acid in yarrowia lipolytica by triton x-100. | various chemical surfactants could affect permeability of yeast cells. in this study, effects of the surfactant addition upon yeast cells permeability and citric acid (ca) production by yarrowia lipolytica strains dsm 3286 and m7 were investigated. the addition of triton x-100 increased 1.4-1.8-fold of the maximum ca quantity achieved for both strains, with final ca concentrations ranging between 75-85 g/l that correspond to ca conversion yields per unit of glucose consumed of ~0.80-0.84 g/g. sc ... | 2011 | 21773802 |
| isolation of a thermostable variant of lip2 lipase from yarrowia lipolytica by directed evolution and deeper insight into the denaturation mechanisms involved. | lip2 lipase from yarrowia lipolytica is a very promising lipase with many potential applications (e.g. resolution of racemic mixtures, production of fine chemicals). unfortunately this potential is impeded by a very low thermostability for temperatures higher than 40°c. error-prone pcr and screening of the library in a high-performance yeast expression system (y. lipolytica) enabled a thermostable variant to be identified. this variant presents only one mutation, the free cysteine 244 is changed ... | 2011 | 21763359 |
| lipid production from yarrowia lipolytica po1g grown in sugarcane bagasse hydrolysate. | this study investigated the possibility of utilizing detoxified sugarcane bagasse hydrolysate (dscbh) as an alternative carbon source to culture yarrowia lipolytica po1g for microbial oil and biodiesel production. sugarcane bagasse hydrolysis with 2.5% hcl resulted in maximum total sugar concentration (21.38g/l) in which 13.59g/l is xylose, 3.98g/l is glucose, and 2.78g/l is arabinose. detoxification of scbh by ca(oh)(2) neutralization reduced the concentration of 5-hydroxymethylfurfural and fur ... | 2011 | 21757339 |
| the intronome of budding yeasts. | whatever their abundance in genomes, spliceosomal introns are the signature of eukaryotic genes. the sequence of saccharomyces-ácerevisiae, achieved fifteen years ago, revealed that this yeast has very few introns, but conserved intron boundaries typical for an intron definition mechanism. with the improvement and the development of new sequencing technologies, yeast genomes have been extensively sequenced during the last decade. we took advantage of this plethora of data to compile and assess t ... | 2011 | 21819948 |
| production of ferulic acid from lignocellulolytic agricultural biomass by thermobifida fusca thermostable esterase produced in yarrowia lipolytica transformant. | a gene (axe) encoding the axe thermostable esterase in thermobifida fusca ntu22 was cloned into a yarrowia lipolytica p01g host strain. recombinant expression resulted in extracellular esterase production at levels as high as 70.94u/ml in hinton flask culture broth, approximately 140 times higher than observed in a pichia pastoris expression system. after 72h of fermentation by the y. lipolytica transformant in the fed-batch fermentor, the fermentation broth accumulated 41.11u/ml esterase activi ... | 2011 | 21683590 |
| expression of a lipid-inducible, self-regulating form of yarrowia lipolytica lipase lip2 in saccharomyces cerevisiae. | saccharomyces cerevisiae is frequently used as a bioreactor for conversion of exogenously acquired metabolites into value-added products, but has not been utilized for bioconversion of low-cost lipids such as triacylglycerols (tags) because the cells are typically unable to acquire these lipid substrates from the growth media. to help circumvent this limitation, the yarrowia lipolytica lipase 2 (lip2) gene was cloned into s. cerevisiae expression vectors and used to generate s. cerevisiae strain ... | 2011 | 21822903 |
| cloning and expression of a tyrosinase from aspergillus oryzae in yarrowia lipolytica: application in l: -dopa biotransformation. | amplification of the tyrosinase gene (melo) from the genomic dna of aspergillus oryzae ncim 1212 yielded a 1.6-kb product. this gene was cloned into pylex1, and the resulting ptyro-ylex1 vector was transformed in yarrowia lipolytica strain po1g. a clone displaying the highest specific activity for tyrosinase (10.94-áu/mg) was used for obtaining the complementary dna (cdna) and for protein expression studies. cdna sequence analysis indicated the splicing of an intron present in the melo gene by p ... | 2011 | 21687965 |
| high efficiency transformation by electroporation of yarrowia lipolytica. | yarrowia lipolytica was usually transformed by heat shock, but linearized integrative vectors always resulted in a low transformation efficiency when electroporation was used. to develop a high efficiency integrative transformation method by electroporation of f. lipolytica, we report here that pretreatment of f. lipolytica with 150 mm liac for 1 h before electroporation will approximately 30-fold of increase transformation efficiency. a cell concentration of 10(10)/ml and instrument settings of ... | 2011 | 21717334 |
| construction and characterization of a yarrowia lipolytica mutant lacking genes encoding cytochromes p450 subfamily 52. | the initial hydroxylation of n-alkane is catalyzed by cytochrome p450alk of the cyp52 family in the n-alkane-assimilating yeast yarrowia lipolytica. a mutant with a deletion of all 12 genes, alk1 to alk12, which are deduced to encode cytochromes p450 of the cyp52 family in y. lipolytica, was successfully constructed. this deletion mutant, δalk1-12, completely lost the ability to grow on n-alkanes of 10-16 carbons. in contrast, δalk1-12 grew on the metabolite of n-dodecane, i.e., n-dodecanol, n-d ... | 2011 | 22119766 |
| fed-batch versus batch cultures of yarrowia lipolytica for γ-decalactone production from methyl ricinoleate. | constant medium feeding rate and intermittent fed-batch fermentation strategies were investigated aiming to increase the yields of γ-decalactone production by yarrowia lipolytica, using methyl ricinoleate as substrate and ricinoleic acid source. the accumulation of another compound, 3-hydroxy-γ-decalactone, was also analyzed since it derives from the direct precursor of γ-decalactone thereby providing information about the enzymatic activities of the pathway. both strategies were compared with t ... | 2011 | 22160330 |
| Effect of prooxidants on yeast mitochondria. | Tightly coupled mitochondria from Yarrowia lipolytica and Dipodascus (Endomyces) magnusii yeasts were used in this study. The two yeasts are aerobes containing the fully competent respiratory chain with three energy conservation sites. Interaction of the yeast mitochondria with prooxidants (diamide, menadione, oxaloacetate, phenylarsine oxide, hydrogen peroxide, t-butyl peroxide, and ascorbate plus Fe(2+)) was studied. The prooxidants, depending on their chemical nature, either caused uncoupling ... | 2011 | 22138819 |