| sugarcane growth promotion by the endophytic bacterium pantoea agglomerans 33.1. | the promotion of sugarcane growth by the endophytic pantoea agglomerans strain 33.1 was studied under gnotobiotic and greenhouse conditions. the green fluorescent protein (gfp)-tagged strain p. agglomerans 33.1::pnkgfp was monitored in vitro in sugarcane plants by microscopy, reisolation, and quantitative pcr (qpcr). using qpcr and reisolation 4 and 15 days after inoculation, we observed that gfp-tagged strains reached similar density levels both in the rhizosphere and inside the roots and aeria ... | 2012 | 22865062 |
| phosphate enhances levan production in the endophytic bacterium gluconacetobacter diazotrophicus pal5. | gluconacetobacter diazotrophicus is a gram-negative and endophytic nitrogen-fixing bacterium that has several beneficial effects in host plants; thus, utilization of this bacterium as a biofertilizer in agriculture may be possible. g. diazotrophicus synthesizes levan, a d-fructofuranosyl polymer with β-(2→6) linkages, as an exopolysaccharide and the synthesized levan improves the stress tolerance of the bacterium. in this study, we found that phosphate enhances levan production by g. diazotrophi ... | 2014 | 24717418 |
| complete genome sequence of kosakonia sacchari type strain sp1(t.). | kosakonia sacchari sp. nov. is a new species within the new genus kosakonia, which was included in the genus enterobacter. k sacchari is a nitrogen-fixing bacterium named for its association with sugarcane (saccharum officinarum l.). k sacchari bacteria are gram-negative, aerobic, non-spore-forming, motile rods. strain sp1(t) (=cgmcc1.12102(t)=lmg 26783(t)) is the type strain of the k sacchari sp. nov and is able to colonize and fix n2 in association with sugarcane plants, thus promoting plant g ... | 2014 | 25197499 |
| epicoccum nigrum p16, a sugarcane endophyte, produces antifungal compounds and induces root growth. | sugarcane is one of the most important crops in brazil, mainly because of its use in biofuel production. recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potential. epicoccum nigrum is an important sugarcane endophytic fungus that has been associated with the biological control of phytopathogens, and the production of secondary metabolites. in spite of several studies carried out to define ... | 2012 | 22675473 |
| an acidic thermostable recombinant aspergillus nidulans endoglucanase is active towards distinct agriculture residues. | aspergillus nidulans is poorly exploited as a source of enzymes for lignocellulosic residues degradation for biotechnological purposes. this work describes the a. nidulans endoglucanase a heterologous expression in pichia pastoris, the purification and biochemical characterization of the recombinant enzyme. active recombinant endoglucanase a (reg a) was efficiently secreted as a 35 kda protein which was purified through a two-step chromatography procedure. the highest enzyme activity was detecte ... | 2013 | 23936633 |
| can commercial digital cameras be used as multispectral sensors? a crop monitoring test. | the use of consumer digital cameras or webcams to characterize and monitor different features has become prevalent in various domains, especially in environmental applications. despite some promising results, such digital camera systems generally suffer from signal aberrations due to the on-board image processing systems and thus offer limited quantitative data acquisition capability. the objective of this study was to test a series of radiometric corrections having the potential to reduce radio ... | 2008 | 27873930 |
| agro-ecosystems impact malaria prevalence: large-scale irrigation drives vector population in western ethiopia. | development strategies in ethiopia have largely focused on the expansion of irrigated agriculture in the last decade to reduce poverty and promote economic growth. however, such irrigation schemes can worsen the socio-economic state by aggravating the problem of mosquito-borne diseases. in this study, the effect of agro-ecosystem practices on malaria prevalence and the risk of malaria transmission by the primary vector mosquito, anopheles arabiensis, in ethiopia were investigated. | 2013 | 24083353 |
| satellite hyperspectral imagery to support tick-borne infectious diseases surveillance. | this study proposed the use of satellite hyperspectral imagery to support tick-borne infectious diseases surveillance based on monitoring the variation in amplifier hosts food sources. to verify this strategy, we used the data of the human rickettsiosis occurrences in southeastern brazil, region in which the emergence of this disease is associated with the rising capybara population. spatio-temporal analysis based on monte carlo simulations was used to identify risk areas of human rickettsiosis ... | 2015 | 26599337 |
| larval habitat associations with human land uses, roads, rivers, and land cover for anopheles albimanus, a. pseudopunctipennis, and a. punctimacula (diptera: culicidae) in coastal and highland ecuador. | larval habitat for three highland anopheles species: anopheles albimanus wiedemann, anopheles pseudopunctipennis theobald, and anopheles punctimacula dyar and knab was related to human land uses, rivers, roads, and remotely sensed land cover classifications in the western ecuadorian andes. of the five commonly observed human land uses, cattle pasture (n = 30) provided potentially suitable habitat for a. punctimacula and a. albimanus in less than 14% of sites, and was related in a principal compo ... | 2012 | 22454623 |
| candidates for symbiotic control of sugarcane white leaf disease. | the leafhopper matsumuratettix hiroglyphicus (matsumura) is the most important vector of a phytoplasma pathogen causing sugarcane white leaf (scwl) disease. the purpose of this study was to evaluate candidate bacterial symbionts for possible use as vehicles in the control of the disease. 16s rrna bacterial genes were amplified from whole bodies of m. hiroglyphicus leafhoppers and analyzed by cloning and sequencing. two dominant groups were found: one belonged to the betaproteobacteria that did n ... | 2012 | 22798373 |
| sugarcane giant borer transcriptome analysis and identification of genes related to digestion. | sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. although its economical relevance, only a few dna sequences are available for this species in the genbank. pyrosequencing technology was used t ... | 2015 | 25706301 |
| silencing of molt-regulating transcription factor gene, cihr3, affects growth and development of sugarcane stem borer, chilo infuscatellus. | rna interference (rnai) is a technology for conducting functional genomic studies and a potential tool for crop protection against insect pests. development of reliable methods for production and delivery of double-stranded rna (dsrna) is the major challenge for efficient pest control. in this study, chilo infuscatellus snellen (crambidae: lepidoptera) was fed with cihr3 dsrna expressed in bacteria or synthesized in vitro. the dsrna ingested by c. infuscatellus successfully triggered silencing o ... | 0 | 23427912 |
| utilization of sugarcane habitat by feral pig (sus scrofa) in northern tropical queensland: evidence from the stable isotope composition of hair. | feral pigs (sus scrofa) are an invasive species that disrupt ecosystem functioning throughout their introduced range. in tropical environments, feral pigs are associated with predation and displacement of endangered species, modification of habitat, and act as a vector for the spread of exotic vegetation and disease. across many parts of their introduced range, the diet of feral pigs is poorly known. although the remote location and difficult terrain of far north queensland makes observing feral ... | 2012 | 22957029 |
| comparison digestibility and protozoa population of khuzestan water buffalo and holstein cow. | the major aim of this study was to compare the morphology and activity of rumen protozoa of khuzestan water buffalo and holstein cow using in vitro digestibility and gas production parameters of steam treated sugarcane pith. rumen fluid obtained from two buffalo and cow steers fed the same diet, 30:70 concentrate: forage. to separate rumen protozoa, antibiotic solution and fungicides were added to rumen fluid. the results of present experiment indicated that the neutral detergent fiber (ndf; 7.8 ... | 0 | 25610581 |
| alleviation of carbon catabolite repression in enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3-butanediol production. | due to its cost-effectiveness and rich sugar composition, sugarcane molasses is considered to be a promising carbon source for biorefinery. however, the sugar mixture in sugarcane molasses is not consumed as efficiently as glucose in microbial fermentation due to complex interactions among their utilizing pathways, such as carbon catabolite repression (ccr). in this study, 2,3-butanediol-producing enterobacter aerogenes was engineered to alleviate ccr and improve sugar utilization by modulating ... | 2015 | 26236395 |
| isolation of cellulolytic bacteria from the intestine of diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass. | as a strategy to find efficient lignocellulose degrading enzymes/microorganisms for sugarcane biomass pretreatment purposes, 118 culturable bacterial strains were isolated from intestines of sugarcane-fed larvae of the moth diatraea saccharalis. all strains were tested for cellulolytic activity using soluble carboxymethyl cellulose (cmc) degrading assays or by growing bacteria on sugarcane biomass as sole carbon sources. out of the 118 strains isolated thirty eight were found to possess cellulos ... | 2015 | 25852992 |
| relationship of soil properties and sugarcane yields to red stripe in louisiana. | symptoms of red stripe disease caused by acidovorax avenae subsp. avenae in louisiana between 1985 and 2010 were limited to the leaf stripe form, which caused no apparent yield loss. during 2010, the more severe top rot form was observed, and a study was initiated to investigate the distribution of red stripe in the field and determine its effects on cane and sugar yields. soil properties data, red stripe incidence, and sugarcane yields were all highly variable and were not randomly distributed ... | 2016 | 27003508 |
| erratum for fontana et al., genome sequence of acidovorax avenae strain t10_61 associated with sugarcane red stripe in argentina. | | 2016 | 26988059 |
| genome sequence of acidovorax avenae strain t10_61 associated with sugarcane red stripe in argentina. | red stripe of sugarcane in argentina is a bacterial disease caused by acidovorax avenae. the genome sequence from the first isolate of this bacterium in argentina is presented here. the draft genome of the a. avenae t10_61 strain contains 5,646,552 bp and has a g+c content of 68.6 mol%. | 2016 | 26847889 |
| enhanced biological straw saccharification through coculturing of lignocellulose-degrading microorganisms. | lignocellulosic waste (lcw) is an abundant, low-cost, and inedible substrate for the induction of lignocellulolytic enzymes for cellulosic bioethanol production using an efficient, environmentally friendly, and economical biological approach. in this study, 30 different lignocellulose-degrading bacterial and 18 fungal isolates were quantitatively screened individually for the saccharification of four different ball-milled straw substrates: wheat, rice, sugarcane, and pea straw. rice and sugarcan ... | 2015 | 25724976 |
| advances in agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops. | steady increase in global population poses several challenges to plant science research, including demand for increased crop productivity, grain yield, nutritional quality and improved tolerance to different environmental factors. transgene-based approaches are promising to address these challenges by transferring potential candidate genes to host organisms through different strategies. agrobacterium-mediated gene transfer is one such strategy which is well known for enabling efficient gene tran ... | 2016 | 26660352 |
| agrobacterium-mediated in planta genetic transformation of sugarcane setts. | an efficient, reproducible, and genotype-independent in planta transformation has been developed for sugarcane using setts as explant. traditional agrobacterium-mediated genetic transformation and in vitro regeneration of sugarcane is a complex and time-consuming process. development of an efficient agrobacterium-mediated transformation protocol, which can produce a large number of transgenic plants in short duration is advantageous. hence, in the present investigation, we developed a tissue cul ... | 2015 | 26152769 |
| sugarcane (saccharum spp. hybrids). | genetic transformation of sugarcane has a tremendous potential to complement traditional breeding in crop improvement and will likely transform sugarcane into a bio-factory for value-added products. we describe here agrobacterium tumefaciens-mediated transformation of sugarcane. embryogenic callus induced from immature leaf whorls was used as target for transformation with the hypervirulent agrobacterium strain agl1 carrying a constitutive nptii expression cassette in vector ppzp200. selection w ... | 2015 | 25416267 |
| application of rna interference methodology to investigate and develop scmv resistance in maize. | specific fragments of the sugarcane mosaic virus (scmv) coat protein gene (cp) were amplified by reverse transcriptionpolymerase chain reaction and used to construct a marker free small interfering rna complex expression vector against scmv. in planta transformation was performed on maize (zea mays) inbred line 8112 mediated by agrobacterium tumefaciens. pcr and southern blot analyses demonstrated successful integration of the cp segment into the 8112 genome. the in planta transformation frequen ... | 2014 | 25189224 |
| physiological and photosynthetic characteristics of indica hang2 expressing the sugarcane pepc gene. | phosphoenolpyruvate carboxylase (pepc) is known to play a key role in the initial fixation of co2 in c4 photosynthesis. the pepc gene from sugarcane (a c4 plant) was introduced into indica rice (hang2), a process mediated by agrobacterium tumefaciens. integration patterns and copy numbers of the gene was confirmed by dna blot analysis. rt-pcr and western blotting results showed that the pepc gene was expressed at both the mrna and protein levels in the transgenic lines. real-time pcr results ind ... | 2014 | 24469712 |
| field performance of transgenic sugarcane produced using agrobacterium and biolistics methods. | future genetic improvement of sugarcane depends, in part, on the ability to produce high-yielding transgenic cultivars with improved traits such as herbicide and insect resistance. here, transgenic sugarcane plants generated by different transformation methods were assessed for field performance over 3 years. agrobacterium-mediated (agro) transgenic events (35) were produced using four different agrobacterium tumefaciens strains, while biolistic (biol) transgenic events (48) were produced using ... | 2014 | 24330327 |
| agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane. | an efficient, reproducible and genotype-independent in planta transformation has been standardized for sugarcane using seed as explant. transgenic sugarcane production through agrobacterium infection followed by in vitro regeneration is a time-consuming process and highly genotype dependent. to obtain more number of transformed sugarcane plants in a relatively short duration, sugarcane seeds were infected with agrobacterium tumefaciens eha 105 harboring pcambia 1304-bar and transformed plants we ... | 2013 | 23749098 |
| identification and characterisation of xylanolytic yeasts isolated from decaying wood and sugarcane bagasse in brazil. | in this study, yeasts associated with lignocellulosic materials in brazil, including decaying wood and sugarcane bagasse, were isolated, and their ability to produce xylanolytic enzymes was investigated. a total of 358 yeast isolates were obtained, with 198 strains isolated from decaying wood and 160 strains isolated from decaying sugarcane bagasse samples. seventy-five isolates possessed xylanase activity in solid medium and were identified as belonging to nine species: candida intermedia, c. t ... | 2014 | 24748334 |
| biomass-to-bio-products application of feruloyl esterase from aspergillus clavatus. | the structural polysaccharides contained in plant cell walls have been pointed to as a promising renewable alternative to petroleum and natural gas. ferulic acid is a ubiquitous component of plant polysaccharides, which is found in either monomeric or dimeric forms and is covalently linked to arabinosyl residues. ferulic acid has several commercial applications in food and pharmaceutical industries. the study herein introduces a novel feruloyl esterase from aspergillus clavatus (acfae). along wi ... | 2013 | 23229566 |
| maize-pathogen interactions: an ongoing combat from a proteomics perspective. | maize (zea mays l.) is a host to numerous pathogenic species that impose serious diseases to its ear and foliage, negatively affecting the yield and the quality of the maize crop. a considerable amount of research has been carried out to elucidate mechanisms of maize-pathogen interactions with a major goal to identify defense-associated proteins. in this review, we summarize interactions of maize with its agriculturally important pathogens that were assessed at the proteome level. employing diff ... | 2015 | 26633370 |
| synthesis, characterization and antimicrobial properties of grafted sugarcane bagasse/silver nanocomposites. | sugarcane bagasse (scb) was grafted with acrylamide (aam) and glycidyl methacrylate (gma) by chemical oxidation method. the effect of monomer/initiator molar ratio, reaction time, reaction temperature and material to liquor ratio on the degree of grafting was investigated. the optimum conditions for grafting were: monomer/initiator molar ratio 1 for aam and 2 for gma, reaction time 4h, reaction temperature 80°c and materials to liquor ratio 1:20. silver nanoparticles (agnps) were prepared and ch ... | 2015 | 25439896 |
| natural aflatoxin uptake by sugarcane (saccharum officinaurum l.) and its persistence in jaggery. | the present study focused on aflatoxin (af) uptake by sugarcanes from contaminated soils, and its persistence in jaggery. analysis of 25 agricultural soil samples from sugarcane growing fields revealed that 80% were found contaminated with af ranging from 0.5 to 22 ppb and all samples harbored aflatoxigenic fungi. forty percent of the juices extracted from sugarcane grown in contaminated soil recorded af ranging from 1.0 to 9.5 ppb. conversely, jaggery prepared from those samples was almost free ... | 2015 | 25408078 |
| association between aflatoxin b1 occupational airway exposure and risk of hepatocellular carcinoma: a case-control study. | the aim of this study was to determine the airway exposure of sugar and papermaking factory workers to aflatoxin b1 (afb1) and to explore the potential association between afb1 airway exposure and the risk of hepatocellular carcinoma (hcc) in a case-control study. dust samples were collected from the sugarcane bagasse warehouse, and presser and paper production workshops. blood samples were collected from 181 workshop employees and 203 controls who worked outside the workshop. afb1 albumin adduc ... | 2014 | 24961349 |
| chemical input reduction in the arabinoxylan and lignocellulose alkaline extraction and xylooligosaccharides production. | lignocellulosic material breakdown by hydrolysis is an important step to open new perspectives for bioenergy and special foods production like prebiotic xylooligosaccharides. improvement of lignocellulose and arabinoxylan alkaline extraction from sugarcane bagasse and enzymatic hydrolysis were performed. treatments 1 (10% koh at 70°c), 3 (5% koh at 121°c) and zd method (24% koh at 35°c) showed solid lignocellulose recovery of respectively 75.2%, 74.2% and 73%. a range of 24.8-27% extracted mater ... | 2017 | 28063358 |
| screening of xylanolytic aspergillus fumigatus for prebiotic xylooligosaccharide production using bagasse. | sugarcane bagasse is an important lignocellulosic material studied for the production of xylooligosaccharides (xos). some xos are considered soluble dietary fibre, with low caloric value and prebiotic effect, but they are expensive and not easily available. in a screening of 138 fungi, only nine were shortlisted, and just aspergillus fumigatus m51 (35.6 u/ml) and a. fumigatus u2370 (28.5 u/ml) were selected as the most significant producers of xylanases. these fungi had low β-xylosidase activity ... | 2015 | 27904377 |
| thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production. | nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at brazilian cerrado and identified up to species level on the basis of the gene sequencing of 5.8s-its and 26s ribosomal dna regions. four species were identified: kluyveromyces marxianus, aspergillus niger, aspergillus sydowii and aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. among them, th ... | 2017 | 26413077 |
| selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation. | twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45°c on medium containing corn straw and cardboard as carbon sources. these fungi were identified in the genera aspergillus, thermomyces, myceliophthora, thermomucor and candida. the majority of the isolated strains produced xylanase and cellulases under solid state fermentation (ssf). the highest cellulase and xylanase productions wer ... | 2012 | 24031929 |
| production of thermophilic endo-β-1,4-xylanases by aspergillus fumigatus fbspe-05 using agro-industrial by-products. | in the present paper, endo-β-1,4-xylanase production by aspergillus fumigatus was evaluated in solid-state fermentation using low-cost substrates such as sugarcane bagasse (scb), brewer's spent grain (bsg), and wheat bran (wb). the partial characterization of the crude enzyme was also performed. in the experimental conditions, the highest levels of endo-β-1,4-xylanase production by a. fumigatus fbspe-05 occurred within 8 days incubation when using scb/liquid medium at 1:2 ratio (219.5 u g(-1)) a ... | 2012 | 22328248 |
| mapping n-linked glycosylation of carbohydrate-active enzymes in the secretome of aspergillus nidulans grown on lignocellulose. | the genus aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (cazymes) that characterize their saprophyte lifestyle. aspergillus has the capacity to perform post-translational modifications (ptm), which provides an additional advantage for the use of these organisms as a host for the production of heterologous proteins. in this study, the n-linked glycosylation of cazymes identified in the secretome of asperg ... | 2016 | 27508003 |
| rnaseq reveals hydrophobins that are involved in the adaptation of aspergillus nidulans to lignocellulose. | sugarcane is one of the world's most profitable crops. waste steam-exploded sugarcane bagasse (seb) is a cheap, abundant, and renewable lignocellulosic feedstock for the next-generation biofuels. in nature, fungi seldom exist as planktonic cells, similar to those found in the nutrient-rich environment created within an industrial fermenter. instead, fungi predominantly form biofilms that allow them to thrive in hostile environments. | 2016 | 27437031 |
| characterization and biotechnological application of recombinant xylanases from aspergillus nidulans. | two xylanases from aspergillus nidulans, xlnb and xlnc, were expressed in pichia pastoris, purified and characterized. xlnb and xlnc achieved maximal activities at 60°c and ph 7.5 and at 50°c and ph 6.0, respectively. xlnb showed to be very thermostable by maintaining 50% of its original activity after 49h incubated at 50°c. xlnb had its highest activity against wheat arabinoxylan while xlnc had the best activity against beechwood xylan. both enzymes were completely inhibited by sds and hgcl2. x ... | 2016 | 27235731 |
| co-cultivation of aspergillus nidulans recombinant strains produces an enzymatic cocktail as alternative to alkaline sugarcane bagasse pretreatment. | plant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. a clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60-80% of fermentable sugars in its composition. however, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. replacing these methods with co-culti ... | 2016 | 27199917 |
| catalytic conversion of sugarcane bagasse to cellulosic ethanol: tio2 coupled nanocellulose as an effective hydrolysis enhancer. | the present study deals with the production of cellulosic ethanol from bagasse using the synthesized tio2 coupled nanocellulose (nc-tio2) as catalyst. aspergillus nidulans ajsu04 cellulase was used for the hydrolysis of bagasse. nc-tio2 at various concentrations was added to bagasse in order to enhance the yield of reducing sugars. complex interaction between cellulase, bagasse, nc-tio2 and the reaction environment is thoroughly studied. a mathematical model was developed to describe the hydroly ... | 2016 | 26572403 |
| gh11 xylanase from emericella nidulans with low sensitivity to inhibition by ethanol and lignocellulose-derived phenolic compounds. | an endo-β-1,4-xylanase (x22) was purified from crude extract of emericella nidulans when cultivated on submerged fermentation using sugarcane bagasse as the carbon source. the purified protein was identified by mass spectrometry and was most active at ph and temperature intervals of 5.0-6.5 and 50-60°c, respectively. the enzyme showed half-lives of 40, 10 and 7 min at 28, 50 and 55°c, respectively, and ph 5.0. apparent km and vmax values on soluble oat spelt xylan were 3.39 mg/ml and 230.8 iu/mg ... | 2015 | 26040589 |
| the sugarcane defense protein sugarwin2 causes cell death in colletotrichum falcatum but not in non-pathogenic fungi. | plants respond to pathogens and insect attacks by inducing and accumulating a large set of defense-related proteins. two homologues of a barley wound-inducible protein (barwin) have been characterized in sugarcane, sugarwin1 and sugarwin2 (sugarcane wound-inducible proteins). induction of sugarwins occurs in response to diatraea saccharalis damage but not to pathogen infection. in addition, the protein itself does not show any effect on insect development; instead, it has antimicrobial activitie ... | 2014 | 24608349 |
| a novel thermostable xylanase gh10 from malbranchea pulchella expressed in aspergillus nidulans with potential applications in biotechnology. | the search for novel thermostable xylanases for industrial use has intensified in recent years, and thermophilic fungi are a promising source of useful enzymes. the present work reports the heterologous expression and biochemical characterization of a novel thermostable xylanase (gh10) from the thermophilic fungus malbranchea pulchella, the influence of glycosylation on its stability, and a potential application in sugarcane bagasse hydrolysis. | 2014 | 25788980 |
| occurrence of fungal species and mycotoxins from decayed sugarcane (saccharrum officinarum) in egypt. | seventy-three fungal species belonging to forty-three genera were isolated from 40 samples of saccharrum officinarum (collected from naage-hamadi canal in qena governorate, egypt). aspergillus, trichoderma, mucor and pythium were the most common genera on the two isolation media. the dominant species of aspergillus were a. niger, a. flavus, a. ustus, a. terreus and a. wentii. some species were dominant on 40 g/l sucrose such as aspergillus niger, a. flavus, emericella nidulans, trichoderma virid ... | 2005 | 24049478 |
| development of minimal enzyme cocktails for hydrolysis of sulfite-pulped lignocellulosic biomass. | despite recent progress, saccharification of lignocellulosic biomass is still a major cost driver in biorefining. in this study, we present the development of minimal enzyme cocktails for hydrolysis of norway spruce and sugarcane bagasse, which were pretreated using the so-called bali™ process, which is based on sulfite pulping technology. minimal enzyme cocktails were composed using several glycoside hydrolases purified from the industrially relevant filamentous fungus trichoderma reesei and a ... | 2017 | 28219736 |
| design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: optimization and synergism studies. | lignocellulosic materials represent a very important and promising source of renewable biomass. in order to turn them into fermentable sugars, synergism among the different enzymes that carry out bioconversion of these materials is one of the main factors that should be considered. experimental mixture design was performed to optimize the proportion of enzymes produced by native strains of trichoderma harzianum ioc 3844, penicillium funiculosum atcc 11797, and aspergillus niger atcc 1004, result ... | 2016 | 27254751 |
| secretome data from trichoderma reesei and aspergillus niger cultivated in submerged and sequential fermentation methods. | the cultivation procedure and the fungal strain applied for enzyme production may influence levels and profile of the proteins produced. the proteomic analysis data presented here provide critical information to compare proteins secreted by trichoderma reesei and aspergillus niger when cultivated through submerged and sequential fermentation processes, using steam-explosion sugarcane bagasse as inducer for enzyme production. the proteins were organized according to the families described in cazy ... | 2016 | 27419196 |
| secretome analysis of trichoderma reesei and aspergillus niger cultivated by submerged and sequential fermentation processes: enzyme production for sugarcane bagasse hydrolysis. | cellulases and hemicellulases from trichoderma reesei and aspergillus niger have been shown to be powerful enzymes for biomass conversion to sugars, but the production costs are still relatively high for commercial application. the choice of an effective microbial cultivation process employed for enzyme production is important, since it may affect titers and the profile of protein secretion. we used proteomic analysis to characterize the secretome of t. reesei and a. niger cultivated in submerge ... | 2016 | 27241292 |
| addition of metal ions to a (hemi)cellulolytic enzymatic cocktail produced in-house improves its activity, thermostability, and efficiency in the saccharification of pretreated sugarcane bagasse. | high activity and stability are essential for (hemi)cellulolytic enzymes used in biomass conversion, while non-productive binding of cellulases to lignin reduces saccharification efficiency and needs to be avoided. one potential strategy is the addition of inexpensive metal ions. this paper describes the influence of divalent metal ions on the activity, thermostability, and saccharification efficiency of (hemi)cellulolytic enzymes produced in-house by aspergillus niger under solid-state fermenta ... | 2016 | 26709004 |
| characterization of the cellulolytic secretome of trichoderma harzianum during growth on sugarcane bagasse and analysis of the activity boosting effects of swollenin. | this study demonstrates the production of an active enzyme cocktail produced by growing trichoderma harzianum on sugarcane bagasse. the component enzymes were identified by lcms-ms. glycosyl hydrolases were the most abundant class of proteins, representing 67% of total secreted protein. other carbohydrate active enzymes involved in cell wall deconstruction included lytic polysaccharide mono-oxygenases (aa9), carbohydrate-binding modules, carbohydrate esterases and swollenin, all present at level ... | 2016 | 26697775 |
| optimization of aspergillus niger rock phosphate solubilization in solid-state fermentation and use of the resulting product as a p fertilizer. | a biotechnological strategy for the production of an alternative p fertilizer is described in this work. the fertilizer was produced through rock phosphate (rp) solubilization by aspergillus niger in a solid-state fermentation (ssf) with sugarcane bagasse as substrate. ssf conditions were optimized by the surface response methodology after an initial screening of factors with significant effect on rp solubilization. the optimized levels of the factors were 865 mg of biochar, 250 mg of rp, 270 mg ... | 2015 | 26112323 |
| comparative secretome analysis of trichoderma reesei and aspergillus niger during growth on sugarcane biomass. | our dependence on fossil fuel sources and concern about the environment has generated a worldwide interest in establishing new sources of fuel and energy. thus, the use of ethanol as a fuel is advantageous because it is an inexhaustible energy source and has minimal environmental impact. currently, brazil is the world's second largest producer of ethanol, which is produced from sugarcane juice fermentation. however, several studies suggest that brazil could double its production per hectare by u ... | 2015 | 26053961 |
| agricultural residues for cellulolytic enzyme production by aspergillus niger: effects of pretreatment. | different agricultural residues were considered in this study for their ability to support cellulolytic enzyme production by aspergillus niger. a total of eleven agricultural residues including finger millet hulls, sorghum hulls, soybean hulls, groundnut husk, banana peels, corn stalk, cassava peels, sugarcane bagasse, saw dust, rice straw and sheanut cake were subjected to three pretreatment (acid, alkali and oxidative) methods. all the residues supported the growth and production of cellulases ... | 2015 | 28324400 |
| saccharification of pretreated sawdust by aspergillus niger cellulase. | the efficiency of two methods of pretreatment (naoh and h2o2) on lignocelluloses-saw dust, wheat straw, sugarcane bagasse and rice bran-was compared in the present study. alkali treatment of lignocelluloses relatively removed more hemicelluloses and lignin leaving behind cellulose content in the residues than peroxide treatment. crude cellulase of aspergillus niger, produced on the pretreated sawdust with highest cellulose content, was further tested for the release of soluble and reducing sugar ... | 2015 | 28324394 |
| liquefaction of sugarcane bagasse for enzyme production. | the objective of this paper is to report liquefaction of pretreated and sterilized sugarcane bagasse for enhancing endoglucanase production through submerged fermentation by aspergillus niger. after initial solid state fermentation of steam pretreated bagasse solids by a. niger, fed-batch addition of the substrate to cellulase in buffer over a 12h period, followed by 36h reaction, resulted in a liquid slurry with a viscosity of 0.30±0.07pas at 30% (w/v) solids. addition of a. niger for submerged ... | 2014 | 25265329 |
| utilization of molasses and sugar cane bagasse for production of fungal invertase in solid state fermentation using aspergillus niger gh1. | agro-industrial wastes have been used as substrate-support in solid state fermentation for enzyme production. molasses and sugarcane bagasse are by-products of sugar industry and can be employed as substrates for invertase production. invertase is an important enzyme for sweeteners development. in this study, a xerophilic fungus aspergillus niger gh1 isolated of the mexican semi-desert, previously reported as an invertase over-producer strain was used. molasses from mexico and cuba were chemical ... | 2014 | 25242918 |
| synergistic effect of aspergillus niger and trichoderma reesei enzyme sets on the saccharification of wheat straw and sugarcane bagasse. | plant-degrading enzymes can be produced by fungi on abundantly available low-cost plant biomass. however, enzymes sets after growth on complex substrates need to be better understood, especially with emphasis on differences between fungal species and the influence of inhibitory compounds in plant substrates, such as monosaccharides. in this study, aspergillus niger and trichoderma reesei were evaluated for the production of enzyme sets after growth on two "second generation" substrates: wheat st ... | 2014 | 25116172 |
| use of spectroscopic and imaging techniques to evaluate pretreated sugarcane bagasse as a substrate for cellulase production under solid-state fermentation. | the enzymatic cocktail of cellulases is one of the most costly inputs affecting the economic viability of the biochemical route for biomass conversion into biofuels and other chemicals. here, the influence of liquid hot water, dilute acid, alkali, and combined acid/alkali pretreatments on sugarcane bagasse (scb) used for cellulase production was investigated by means of spectroscopic and imaging techniques. chemical composition and structural characteristics, such as crystallinity (determined by ... | 2014 | 24363237 |
| the capability of endophytic fungi for production of hemicellulases and related enzymes. | there is an imperative necessity for alternative sources of energy able to reduce the world dependence of fossil oil. one of the most successful options is ethanol obtained mainly from sugarcane and corn fermentation. the foremost residue from sugarcane industry is the bagasse, a rich lignocellulosic raw material uses for the production of ethanol second generation (2g). new cellulolytic and hemicellulytic enzymes are needed, in order to optimize the degradation of bagasse and production of etha ... | 2013 | 24175970 |
| saccharification of biomass using whole solid-state fermentation medium to avoid additional separation steps. | the enzymatic hydrolysis of steam-exploded sugarcane bagasse (sesb) was investigated using enzymatic extracts (ee) and whole fermentation media (wm), produced in-house, from aspergillus niger 3t5b8 and trichoderma reesei rut-c30 cultivated on wheat bran under solid-state fermentation (ssf). a detailed and quantitative comparison of the different hydrolysis conditions tested was carried out using the chrastil approach for modeling enzymatic reactions by fitting the experimental data of total redu ... | 2013 | 24115639 |
| the influence of aspergillus niger transcription factors arar and xlnr in the gene expression during growth in d-xylose, l-arabinose and steam-exploded sugarcane bagasse. | the interest in the conversion of plant biomass to renewable fuels such as bioethanol has led to an increased investigation into the processes regulating biomass saccharification. the filamentous fungus aspergillus niger is an important microorganism capable of producing a wide variety of plant biomass degrading enzymes. in a. niger the transcriptional activator xlnr and its close homolog, arar, controls the main (hemi-)cellulolytic system responsible for plant polysaccharide degradation. sugarc ... | 2013 | 23892063 |
| effect of forced aeration on citric acid production by aspergillus sp. mutants in ssf. | citric acid (ca) is one of the most important products of fermentation in the world. a great variety of agro-industrial residues can be used in solid state fermentation. aspergillus niger parental strain (cct 7716) and two strains obtained by mutagenesis (cct 7717 and cct 7718) were evaluated in erlenmeyer flasks and glass columns using citric pulp (cp) as substrate/support, sugarcane molasses and methanol. best results using glass columns (forced aeration) were found in the fourth day of fermen ... | 2013 | 23760557 |
| fungal rock phosphate solubilization using sugarcane bagasse. | the effects of different doses of rock phosphate (rp), sucrose, and (nh(4))(2)so(4) on the solubilization of rp from araxá and catalão (brazil) by aspergillus niger, penicillium canescens, eupenicillium ludwigii, and penicillium islandicum were evaluated in a solid-state fermentation (ssf) system with sugarcane bagasse. the factors evaluated were combined following a 2(3) + 1 factorial design to determine their optimum concentrations. the fitted response surfaces showed that higher doses of rp p ... | 2013 | 22927013 |
| production of cellulases from aspergillus niger ns-2 in solid state fermentation on agricultural and kitchen waste residues. | various agricultural and kitchen waste residues were assessed for their ability to support the production of a complete cellulase system by aspergillus niger ns-2 in solid state fermentation. untreated as well as acid and base-pretreated substrates including corn cobs, carrot peelings, composite, grass, leaves, orange peelings, pineapple peelings, potato peelings, rice husk, sugarcane bagasse, saw dust, wheat bran, wheat straw, simply moistened with water, were found to be well suited for the or ... | 2012 | 22503148 |
| sequential solid-state and submerged cultivation of aspergillus niger on sugarcane bagasse for the production of cellulase. | sequential solid-state and submerged cultivation with sugarcane bagasse as substrate for cellulase production by aspergillus niger a12 was assessed by measuring endoglucanase activity. an unconventional pre-culture with an initial fungal growth phase under solid-state cultivation was followed by a transition to submerged fermentation by adding the liquid culture medium to the mycelium grown on solid substrate. for comparison, control experiments were conducted using conventional submerged cultiv ... | 2012 | 22409979 |
| heterologous expression of manganese peroxidase in aspergillus niger and its effect on phenanthrene removal from soil. | a strain of aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. transformants were selected based on their resistance to hygromycin b and the discoloration induced on poly r-478 dye by the peroxidase activity. the recombinant a. niger sbc2-t3 stra ... | 2011 | 22286039 |
| sugarcane-pressmud as a novel substrate for production of citric acid by solid-state fermentation. | sugarcane-pressmud, a by-product of cane-sugar manufacture, was used as a substrate for production of citric acid by aspergillus niger cftri 30, in a solid-state fermentation system. of the 170 g of sugar supplied, 131 g were consumed, with a 79% yield of citric acid over 120 h. potassium ferrocyanide improved the conversion to about 88% and lowered the fermentation time by 24 h. enrichment with sugar and nh4no3 was essential to improve productivity. about 174 g citric acid/kg dry sugarcane-pres ... | 1993 | 24420047 |
| production of fructooligosaccharides from sucrose by a transfructosylase from aspergillus niger. | a strain of aspergillus niger isolated from sugarcane fields, produced an extracellular transfructosylase in the culture medium. sucrose and raffinose induced the production to the enzyme, which was purified by 138-fold. the optimum ph for activity and stability were 5.5 and 6.5, respectively. its optimum temperature was 55°c. the enzyme hydrolysed sucrose rapidly and simultaneously formed fructooligosaccharides by transfructosylation. | 1991 | 24425020 |
| isolation and characterisation of a ferrirhodin synthetase gene from the sugarcane pathogen fusarium sacchari. | fsn1, a gene isolated from the sugar-cane pathogen fusarium sacchari, encodes a 4707-residue nonribosomal peptide synthetase consisting of three complete adenylation, thiolation and condensation modules followed by two additional thiolation and condensation domain repeats. this structure is similar to that of ferricrocin synthetase, which makes a siderophore that is involved in intracellular iron storage in other filamentous fungi. heterologous expression of fsn1 in aspergillus oryzae resulted i ... | 2013 | 23307607 |
| production of cellulase from aspergillus terreus ms105 on crude and commercially purified substrates. | aspergillus terreus ms105 was originally isolated from soil and screened for cellulase production in the presence of various carbon sources including carboxymethyl cellulose (cmc), avicel, sigmacell, filter-paper and salicin. cmc induced the production of endoglucanase (eg) and filter-paperase while the levels of β-glucosidase (bgl) were increased when salicin was present in the medium. nature of production medium influenced the duration of lag- and log-phase of the growth, rate of fungal dry-ma ... | 2016 | 28330173 |
| simultaneous saccharification and fermentation of delignified lignocellulosic biomass at high solid loadings by a newly isolated thermotolerant kluyveromyces sp. for ethanol production. | simultaneous saccharification and fermentation studies were carried out using thermotolerant newly isolated kluyveromyces sp. with three different delignified lignocellulosic biomass viz. rice straw, wheat straw and sugarcane bagasse at 5-15% solid loading and 6-12 fpu g(-1) substrate enzyme loading for different time intervals 0-72 h at 42°c. maximum ethanol achieved from rice straw, wheat straw and sugarcane bagasse with in-house crude cellulases from aspergillus terreus was 23.23, 18.29 and 1 ... | 2015 | 25553563 |
| two β-xylanases from aspergillus terreus: characterization and influence of phenolic compounds on xylanase activity. | sugarcane bagasse was used as an inexpensive alternative carbon source for production of β-xylanases from aspergillus terreus. the induction profile showed that the xylanase activity was detected from the 6th day of cultivation period. two low molecular weight enzymes, named xyl t1 and xyl t2 were purified to apparent homogeneity by ultrafiltration, gel filtration and ion exchange chromatographies and presented molecular masses of 24.3and 23.60 kda, as determined by sds-page, respectively. xyl t ... | 2013 | 23892064 |
| coupled production of single cell oil as biodiesel feedstock, xylitol and xylanase from sugarcane bagasse in a biorefinery concept using fungi from the tropical mangrove wetlands. | this work evaluates sugarcane bagasse (scb) conversion, in a biorefinery approach, to coproduce biodiesel and high value products using two novel mangrove fungi. on acid pre-treatment, sugarcane bagasse hydrolysate (scbh) resulted in a xylitol yield of 0.51 g/g xylose consumed in 72 h by williopsis saturnus. after scb pretreatment, sugarcane bagasse residue (scbr) was utilized using aspergillus terreus for production of xylanase (12.74 u/ml) and cell biomass (9.8 g/l) which was extracted for sin ... | 2013 | 23260270 |
| single cell oil of oleaginous fungi from the tropical mangrove wetlands as a potential feedstock for biodiesel. | single cell oils (scos) accumulated by oleaginous fungi have emerged as a potential alternative feedstock for biodiesel production. though fungi from mangrove ecosystem have been reported for production of several lignocellulolytic enzymes, they remain unexplored for their sco producing ability. thus, these oleaginous fungi from the mangrove ecosystem could be suitable candidates for production of scos from lignocellulosic biomass. the accumulation of lipids being species specific, strain select ... | 2012 | 22646719 |
| low-melanin containing pullulan production from sugarcane bagasse hydrolysate by aureobasidium pullulans in fermentations assisted by light-emitting diode. | pullulan is a polymer produced by aureobasidium pullulans and the main bottleneck for its industrial production is the presence of melanin pigment. in this study, light-emitting diodes (leds) of different wavelengths were used to assist the fermentation process aiming to produce low-melanin containing pullulan by wild strain of a. pullulans lb83 with different carbon sources. under white light using glucose-based medium, 11.75g.l(-1) of pullulan with high melanin content (45.70ua540nm.g(-1)) was ... | 2017 | 28161623 |
| production of poly(malic acid) from sugarcane juice in fermentation by aureobasidium pullulans: kinetics and process economics. | poly(β-l-malic acid) (pma) is a biodegradable polymer with many potential biomedical applications. pma can be readily hydrolyzed to malic acid (ma), which is widely used as an acidulant in foods and pharmaceuticals. pma production from sucrose and sugarcane juice by aureobasidium pullulans zx-10 was studied in shake-flasks and bioreactors, confirming that sugarcane juice can be used as an economical substrate without any pretreatment or nutrients supplementation. a high pma titer of 116.3g/l and ... | 2017 | 27839861 |
| biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of himalayan region. | seven endophytic bacterial isolates were finally recovered from native sugarcane varieties at hilly areas namely berinag, champawat and didihat of uttarakhand state in northern himalayan region. new isolates and two standard cultures-azospirillum brasilense and gluconacetobacter diazotrophicus, were evaluated for their morphological, biochemical and molecular characteristics. morphologically all were rod shaped, gram-negative bacteria. their plant growth promotory properties were also assessed w ... | 2013 | 28324369 |
| biodegradation of imidacloprid by consortium of two soil isolated bacillus sp. | imidacloprid degradation potential of bacterial cultures from sugarcane growing soils was studied in liquid culture and bacillus aerophilus and bacillus alkalinitrilicus showed maximum potential to degrade imidacloprid. hence, into a clay loam soil imidacloprid was added at 50, 100, and 150 mg kg(-1) along with 45 × 10(7) cells g(-1) soil of both species under autoclaved and unautoclaved conditions. under autoclaved conditions imidacloprid residues were degraded after 56 days to 3.18, 5.83 and 1 ... | 2014 | 25257222 |
| isolation and characterization of novel phorate-degrading bacterial species from agricultural soil. | based upon 16s rdna sequence homology, 15 phorate-degrading bacteria isolated from sugarcane field soils by selective enrichment were identified to be different species of bacillus, pseudomonas, brevibacterium, and staphylococcus. relative phorate degradation in a mineral salt medium containing phorate (50 μg ml(-1)) as sole carbon source established that all the bacterial species could actively degrade more than 97 % phorate during 21 days. three of these species viz. bacillus aerophilus strain ... | 2014 | 24046230 |
| xylanases of bacillus spp. isolated from ruminant dung as potential accessory enzymes for agro-waste saccharification. | polysaccharide hydrolase producing bacteria were isolated for biomass saccharification step in two-step bioethanol production. xylanolytic bacteria were found to be common in ruminant dung. seven bacillus dung isolates exhibited high xylanolytic activity; three of which were identified as bacillus safensis and four as bacillus altitudinis, based on 16s rdna and gyrb gene sequencing. interestingly, comparison of activity profiles for b. safensis m35 and b. altitudinis r31 and j208 crude xylanases ... | 2015 | 25645626 |
| draft genome sequence of bacillus amyloliquefaciens jjc33m, isolated from sugarcane soils in the papaloapan region, mexico. | bacillus amyloliquefaciens strain jjc33m is a bacterium that produces α-amylase (ec 3.2.1.1) and was isolated from sugarcane soil. its estimated genome size is 3.96 mb, and it harbors 4,048 coding genes (cdss). | 2015 | 25676753 |
| construction of individual, fused, and co-expressed proteins of endoglucanase and β-glucosidase for hydrolyzing sugarcane bagasse. | at least a combination of endoglucanase (eglii) and β-glucosidase (bglz) is required for hydrolyzing crystalline cellulose. to understand the catalytic efficiency of combination enzymes for converting biomass to sugars, eglii and bglz were constructed in the form of individual, fused as well as co-expression proteins, and their activities for hydrolyzing sugarcane bagasse were evaluated. the genes, eglii isolated from bacillus amyloliquefaciens psm3.1 earlier and bglz from b. amyloliquefaciens a ... | 2016 | 24598011 |
| relations between phenotypic changes of spores and biofilm production by bacillus atrophaeus atcc 9372 growing in solid-state fermentation. | bacillus spp. spores are usually obtained from strains cultivated in artificial media. however, in natural habitats, spores are predominantly formed from bacteria present in highly surface-associated communities of cells. solid-state fermentation (ssf) is the culture method that best mimetizes the natural environment of many microorganisms that grow attached to the surface of solid particles. this study aims to confirm that sporulation through ssf of bacillus atrophaeus occurs by biofilm formati ... | 2012 | 22526268 |
| alleviation of heavy metals toxicity by the application of plant growth promoting rhizobacteria and effects on wheat grown in saline sodic field. | the aim of the study was to determine tolerance of plant growth promoting rhizobacteria (pgpr) in different concentrations of cu, cr, co, cd, ni, mn, and pb and to evaluate the pgpr-modulated bioavailability of different heavy metals in the rhizosphere soil and wheat tissues, grown in saline sodic soil. bacillus cereus and pseudomonas moraviensis were isolated from cenchrus ciliaris l. growing in the khewra salt range. seven-day-old cultures of pgpr were applied on wheat as single inoculum, co-i ... | 2017 | 27936865 |
| production of l(+)-lactic acid from acid pretreated sugarcane bagasse using bacillus coagulans dsm2314 in a simultaneous saccharification and fermentation strategy. | sugars derived from lignocellulose-rich sugarcane bagasse can be used as feedstock for production of l(+)-lactic acid, a precursor for renewable bioplastics. in our research, acid-pretreated bagasse was hydrolysed with the enzyme cocktail gc220 and fermented by the moderate thermophilic bacterium bacillus coagulans dsm2314. saccharification and fermentation were performed simultaneously (ssf), adding acid-pretreated bagasse either in one batch or in two stages. ssf was performed at low enzyme do ... | 2016 | 27872661 |
| prospecting agro-waste cocktail: supplementation for cellulase production by a newly isolated thermophilic b. licheniformis 2d55. | bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. the cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for biofuel production. among three potential bacteria examined, bacillus licheniformis 2d55 (accession no. kt799651) was found to produce the highest cellulolytic activity (cmcase 0.3 ... | 2017 | 28176140 |
| expression of thermostable β-xylosidase in escherichia coli for use in saccharification of plant biomass. | the present work is aimed to evaluate the saccharification potential of a thermostable β-xylosidase cloned from bacillus licheniformis into escherichia coli for production of bioethanol from plant biomass. recombinant β-xylosidase enzyme possesses the ability of bioconversion of plant biomass like wheat straw, rice straw and sugarcane bagass. by using this approach, plant biomass that mainly constitute cellulose can be converted to reducing sugars that could then be easily converted to bioethano ... | 2017 | 28140759 |
| optimization of levan production by cold-active bacillus licheniformis ant 179 and fructooligosaccharide synthesis by its levansucrase. | fructooligosaccharides (fos) and levan attract much attention due to a wide range of applications in food technology and pharmaceutical and cosmetic industry. bacillus licheniformis ant 179, isolated from antarctica soil, produced levansucrase and levan in a medium containing sucrose as carbon substrate. in this study, characterization of levansucrase and production of short-chain fos and levan were investigated. temperature and ph optimum of the enzyme were found to be 60 °c and ph 6.0, respect ... | 2017 | 27734288 |
| gh53 endo-beta-1,4-galactanase from a newly isolated bacillus licheniformis cbmai 1609 as an enzymatic cocktail supplement for biomass saccharification. | galactanases (endo-β-1,4-galactanases-ec 3.2.1.89) catalyze the hydrolysis of β-1,4 galactosidic bonds in arabinogalactan and galactan side chains found in type i rhamnogalacturan. the aim of this work was to understand the catalytic function, biophysical properties, and use of a recombinant gh53 endo-beta-1,4-galactanase for commercial cocktail supplementation. the nucleotide sequence of the endo-β-1,4-galactanase from bacillus licheniformis cbmai 1609 (bl1609gal) was cloned and expressed in es ... | 2016 | 26879978 |
| poly-γ-glutamic acid produced from bacillus licheniformis cgmcc 2876 as a potential substitute for polyacrylamide in the sugarcane industry. | as an environmentally friendly and industrially useful biopolymer, poly-γ-glutamic acid (γ-pga) from bacillus licheniformis cgmcc 2876 was characterized by the high-resolution mass spectrometry and (1)h nmr. a flocculating activity of 11,474.47 u ml(-1) obtained with γ-pga, and the effects of carbon sources, ions, and chemical properties (d-/l-composition and molecular weight) on the production and flocculating activity of γ-pga were discussed. being a bioflocculant in the sugar refinery process ... | 2015 | 26033934 |
| increased biomass saccharification by supplementation of a commercial enzyme cocktail with endo-arabinanase from bacillus licheniformis. | the use of endo-arabinanase from bacillus licheniformis (abnase) for sugarcane saccharification has been evaluated by enzyme immobilization and commercial cocktail supplement with the immobilized heterologous protein. | 2015 | 25801671 |
| enhancing polyhydroxybutyrate production from high cell density fed-batch fermentation of bacillus megaterium ba-019. | this study demonstrated the improved polyhydroxybutyrate (phb) production via high cell density cultivation of bacillus megaterium ba-019 with balanced initial total sugar concentration and carbon to nitrogen (c/n) weight ratio. in the 10 l stirred fermentor operated at 30 °c, ph 7.0, 600 rpm, and 1.0 vvm air, with the initial total sugar concentration of 60 g/l and urea at the c/n weight ratio of 10:1, 32.48 g/l cell biomass with the corresponding phb weight content of 26.94 % and volumetric pr ... | 2013 | 23340799 |
| cloning, expression, and purification of xylanase gene from bacillus licheniformis for use in saccharification of plant biomass. | the xylanase gene (xyna) of bacillus licheniformis 9945a was cloned and expressed in escherichia coli bl21(de3) using pet-22b(+) as an expression vector. the recombinant xylanase enzyme was purified by ammonium sulfate precipitation, followed by single-step immobilized metal ion affinity chromatography with a 57.58-fold purification having 138.2 u/mg specific activity and recovery of 70.08 %. molecular weight of the purified xylanase, 23 kda, was determined by sodium dodecyl sulfate polyacrylami ... | 2016 | 26438315 |
| sugarcane bagasse as support for immobilization of bacillus pumilus hz-2 and its use in bioremediation of mesotrione-contaminated soils. | the degrading microorganisms isolated from environment usually fail to degrade pollutants when used for bioremediation of contaminated soils; thus, additional treatments are needed to enhance biodegradation. in the present study, the potential of sugarcane bagasse as bacteria-immobilizing support was investigated in mesotrione biodegradation. a novel isolate bacillus pumilus hz-2 was applied in bacterial immobilization, which was capable of degrading over 95 % of mesotrione at initial concentrat ... | 2015 | 26337896 |
| proteomic profile of hemolymph and detection of induced antimicrobial peptides in response to microbial challenge in diatraea saccharalis (lepidoptera: crambidae). | insects are organisms extremely well adapted to diverse habitats, primarily due to their innate immune system, which provides them with a range of cellular and humoral responses against microorganisms. lepidoptera hemolymph proteins involved in humoral responses are well known; however, there is a lack of knowledge about the sugarcane borer diatraea saccharalis. in this present work, the hemolymph proteins of this pest insect were studied by applying proteomic methodologies. two-dimensional elec ... | 2016 | 27012208 |
| xylan-specific carbohydrate-binding module belonging to family 6 enhances the catalytic performance of a gh11 endo-xylanase. | xylanases catalyze the hydrolysis of β-1,4-linked xylosyl moieties from xylan chains, one of the most abundant hemicellulosic polysaccharides found in plant cell walls. these enzymes can exist either as single catalytic domains or as modular proteins composed of one or more carbohydrate-binding modules (cbms) appended to the catalytic core. however, the molecular mechanisms governing the synergistic effects between catalytic domains and their cbms are not fully understood. thus, the goal of this ... | 2016 | 26923808 |
| engineering of bacillus subtilis for the production of 2,3-butanediol from sugarcane molasses. | 2,3-butanediol is known to be a platform chemical with several potential industrial applications. sustainable industrial scale production can be attained by using a sugarcane molasses based fermentation process using bacillus subtilis. however, the accumulation of acetoin needs to be reduced to improve process efficiency. in this work, b. subtilis was genetically modified in order to increase the yield of 2,3-butanediol. metabolic engineering strategies such as cofactor engineering and overexpre ... | 2016 | 26825987 |