| transcriptional profiling of sweetpotato (ipomoea batatas) roots indicates down-regulation of lignin biosynthesis and up-regulation of starch biosynthesis at an early stage of storage root formation. | the number of fibrous roots that develop into storage roots determines sweetpotato yield. the aim of the present study was to identify the molecular mechanisms involved in the initiation of storage root formation, by performing a detailed transcriptomic analysis of initiating storage roots using next-generation sequencing platforms. a two-step approach was undertaken: (1) generating a database for the sweetpotato root transcriptome using 454-roche sequencing of a cdna library created from pooled ... | 2013 | 23834507 |
| in vivo wound healing and antiulcer properties of white sweet potato (ipomoea batatas). | the potential of tuber flour of ipomoea batatas (l.) lam. cv. brazlândia branca (white sweet potato) as wound healing and antiulcerogenic agent was investigated in vivo in animal model. excision on the back of wistar rats was performed to induce wounds that were topically treated with beeler's base containing tuber flour of white sweet potato at 2.5%. number of cells undergoing metaphase and the degree of tissue re-epithelialization were investigated 4, 7 and 10 days post-treatment. the protecti ... | 2013 | 25685447 |
| disentangling the origins of cultivated sweet potato (ipomoea batatas (l.) lam.). | sweet potato (ipomoea batatas (l.) lam., convolvulaceae) counts among the most widely cultivated staple crops worldwide, yet the origins of its domestication remain unclear. this hexaploid species could have had either an autopolyploid origin, from the diploid i. trifida, or an allopolyploid origin, involving genomes of i. trifida and i. triloba. we generated molecular genetic data for a broad sample of cultivated sweet potatoes and its diploid and polyploid wild relatives, for noncoding chlorop ... | 2013 | 23723970 |
| interaction of small rna-8105 and the intron of ibmyb1 rna regulates ibmyb1 family genes through secondary sirnas and dna methylation after wounding. | small rnas (srnas) play important roles in plants under stress conditions. however, limited research has been performed on the srnas involved in plant wound responses. in the present study, a novel wounding-induced srna, srna8105, was identified in sweet potato (ipomoea batatas cv. tainung 57) using microarray analysis. it was found that expression of srna8105 increased after mechanical wounding. furthermore, dicer-like 1 (dcl1) is required for the srna8105 precursor (pre-srna8105) to generate 2 ... | 2013 | 23663233 |
| on the origin of sweet potato (ipomoea batatas (l.) lam.) genetic diversity in new guinea, a secondary centre of diversity. | new guinea is considered the most important secondary centre of diversity for sweet potato (ipomoea batatas). we analysed nuclear and chloroplast genetic diversity of 417 new guinea sweet potato landraces, representing agro-morphological diversity collected throughout the island, and compared this diversity with that in tropical america. the molecular data reveal moderate diversity across all accessions analysed, lower than that found in tropical america. nuclear data confirm previous results, s ... | 2013 | 23531982 |
| ibacp, a sixteen-amino-acid peptide isolated from ipomoea batatas leaves, induces carcinoma cell apoptosis. | a 16-amino-acid peptide was isolated from the leaves of sweet potato. the peptide caused a rapid alkalinization response in tomato suspension culture media, a characteristic of defense peptides in plants. no post-translational modification was observed on the peptide according to maldi-ms analysis. we have named the peptide ipomoea batatas anti-cancer peptide (ibacp). ibacp also was shown with the ability to dose-dependently inhibit panc-1, a pancreatic cancer line, cell proliferation. the morph ... | 2013 | 23428969 |
| attack of two new spider mites on sweet potato (ipomoea batatas) in diamantina, minas gerais state, brazil. | | 2012 | 23295532 |
| stable internal reference genes for the normalization of real-time pcr in different sweetpotato cultivars subjected to abiotic stress conditions. | reverse transcription quantitative real-time pcr (rt-qpcr) has become one of the most widely used methods for gene expression analysis, but its successful application depends on the stability of suitable reference genes used for data normalization. in plant studies, the choice and optimal number of reference genes must be experimentally determined for the specific conditions, plant species, and cultivars. in this study, ten candidate reference genes of sweetpotato (ipomoea batatas) were isolated ... | 2012 | 23251557 |
| [chemical constituents from the tubers of ipomoea batata]. | to investigate the chemical constituents from the tubers of ipomoea batata. | 2012 | 23236827 |
| chemical optimization of protein extraction from sweet potato (ipomoea batatas) peel. | proteins isolated from sweet potatoes (ipomoea batatas) have been shown to possess antidiabetic, antioxidant, and antiproliferative properties. the objective of this study was to chemically optimize a process for extracting proteins from sweet potato peel. the extraction procedure involved mixing peel with saline solvent to dissolve proteins and then precipitating with cacl(2). quadratic and segmented models were used to determine the optimum nacl concentration and peel to solvent ratio to maxim ... | 2012 | 23163903 |
| genetic diversity and recombination analysis of sweepoviruses from brazil. | monopartite begomoviruses (genus begomovirus, family geminiviridae) that infect sweet potato (ipomoea batatas) around the world are known as sweepoviruses. because sweet potato plants are vegetatively propagated, the accumulation of viruses can become a major constraint for root production. mixed infections of sweepovirus species and strains can lead to recombination, which may contribute to the generation of new recombinant sweepoviruses. | 2012 | 23082767 |
| a modified template for microtia reconstruction tested by surgical simulation on ipomoea batatas. | | 2012 | 23018734 |
| effects of feeding sweet potato (ipomoea batatas) vines as a supplement on feed intake, growth performance, digestibility and carcass characteristics of sidama goats fed a basal diet of natural grass hay. | the objective of this study was to investigate the effects of substituting sweet potato [ipomoea batatas (l) lam] vines for concentrate on growth performance, digestibility, and carcass characteristics. thirty yearling bucks (15.3 ± 1.64 kg) were assigned into six treatments in a randomized complete block design: natural grass hay alone (t1) or supplemented with 100 % sweet potato vines (spv) (t2), 65 % spv + 35 % concentrate (t3), 35 % spv + 65 % concentrate (t4), and 100 % concentrate (t5) on ... | 2013 | 22987286 |
| defensin protein from sweet potato (ipomoea batatas [l.] lam 'tainong 57') storage roots exhibits antioxidant activities in vitro and ex vivo. | this study was designed to investigate the antioxidant activities of sweet potato defensin (spd1) in vitro and ex vivo. antioxidant status [2,2'-azinobis[3-ethylbenzthiazoline-6-sulphonic acid (abts) assay], scavenging activity against dpph (1,1-dipheny-2-picrylhydrazyl) radical method, reducing power method, fe(2+)-chelating ability, ftc (ferric thiocyanate) method, and protection of calf thymus dna against hydroxyl radical-induced damage were studied in vitro. the ex vivo experiments revealed ... | 2012 | 22953797 |
| down-regulation of the ibexp1 gene enhanced storage root development in sweetpotato. | the role of an expansin gene (ibexp1) in the formation of the storage root (sr) was investigated by expression pattern analysis and characterization of ibexp1-antisense sweetpotato (ipomoea batatas cv. yulmi) plants in an attempt to elucidate the molecular mechanism underlying sr development in sweetpotato. the transcript level of ibexp1 was high in the fibrous root (fr) and petiole at the fr stage, but decreased significantly at the young storage root (ysr) stage. ibexp1-antisense plants cultur ... | 2013 | 22945944 |
| micror828 regulates lignin and h2o2 accumulation in sweet potato on wounding. | micrornas (mirnas) are small noncoding rnas which post-transcriptionally regulate gene expression by directing mrna cleavage or translational inhibition. mirnas play multiple roles in the growth, development and stress responses in plants. however, little is known of the wounding-responsive mirnas and their regulation. here, we investigated the expression patterns of micror828 (mir828) on wounding in sweet potato (ipomoea batatas cv tainung 57). the expression of mir828 was only detected in leav ... | 2012 | 22931461 |
| ipomoelin, a jacalin-related lectin with a compact tetrameric association and versatile carbohydrate binding properties regulated by its n terminus. | many proteins are induced in the plant defense response to biotic stress or mechanical wounding. one group is lectins. ipomoelin (ipo) is one of the wound-inducible proteins of sweet potato (ipomoea batatas cv. tainung 57) and is a jacalin-related lectin (jrl). in this study, we resolved the crystal structures of ipo in its apo form and in complex with carbohydrates such as methyl α-d-mannopyranoside (me-man), methyl α-d-glucopyranoside (me-glc), and methyl α-d-galactopyranoside (me-gal) in diff ... | 2012 | 22808208 |
| stability of carotenoids, total phenolics and in vitro antioxidant capacity in the thermal processing of orange-fleshed sweet potato (ipomoea batatas lam.) cultivars grown in brazil. | intervention strategies regarding the biofortification of orange-fleshed sweet potato, which is a rich source of carotenoids for combating vitamin a deficiency, are being developed in brazil. this study was conducted to evaluate the concentrations of individual carotenoids, total phenolic compounds and antioxidant capacity in the roots of four biofortified sweet potato cultivars that were raw or processed by four common heat treatments. hplc, folin-ciocalteu, dpph and abts assays were used. all ... | 2012 | 22802046 |
| advances in functional use of sweet potato, [ipomoea batatas (l.) lam]. | this article reviews the patents that have been presented over the past two decades related to alternative functional use of the sweet potato, ipomoea batatas (l.) lam. the major categories of available patents include alternative food products such as sweet potato chips and fries, sweet potato ornamental products, and fuel ethanol production from sweet potato. the majority of recent patents fall under the category of ornamental products and alternative food products, with only a few fuel ethano ... | 2012 | 22741930 |
| improved tolerance to various abiotic stresses in transgenic sweet potato (ipomoea batatas) expressing spinach betaine aldehyde dehydrogenase. | abiotic stresses are critical delimiters for the increased productivity and cultivation expansion of sweet potato (ipomoea batatas), a root crop with worldwide importance. the increased production of glycine betaine (gb) improves plant tolerance to various abiotic stresses without strong phenotypic changes, providing a feasible approach to improve stable yield production under unfavorable conditions. the gene encoding betaine aldehyde dehydrogenase (badh) is involved in the biosynthesis of gb in ... | 2012 | 22615986 |
| digital gene expression analysis based on integrated de novo transcriptome assembly of sweet potato [ipomoea batatas (l.) lam]. | sweet potato (ipomoea batatas l. [lam.]) ranks among the top six most important food crops in the world. it is widely grown throughout the world with high and stable yield, strong adaptability, rich nutrient content, and multiple uses. however, little is known about the molecular biology of this important non-model organism due to lack of genomic resources. hence, studies based on high-throughput sequencing technologies are needed to get a comprehensive and integrated genomic resource and better ... | 2012 | 22558397 |
| a proteomic analysis of storage stress responses in ipomoea batatas (l.) lam. tuberous root. | during post-harvest storage, tuberous roots of sweet potato (ipomoea batatas l. lam.) usually undergo a biotic and abiotic stress influencing protein expression pattern and substance contents. this research compared the change of total proteins and carbohydrate content in tuberous roots of sweet potato during the storage period. the result of the two-dimensional electrophoresis analysis demonstrated that there were 25 differentially expressed proteins between day 0 and day 75 during the storage. ... | 2012 | 22547271 |
| molecular characterization of two ethylene response factor genes in sweetpotato that respond to stress and activate the expression of defense genes in tobacco leaves. | two ethylene response factor (erf) genes, iberf1 and iberf2, were isolated from a library of expressed sequence tags (est) prepared from suspension-cultured cells and dehydration-treated fibrous roots of sweetpotato (ipomoea batatas). the deduced iberfs contained a nuclear localization signal and the ap2/erf dna-binding domain. rt-pcr analysis revealed that iberf1 was expressed abundantly during the growth of suspension-cultured cells, whereas the expression levels of iberf2 transcripts were hig ... | 2012 | 22459326 |
| sweet potato for type 2 diabetes mellitus. | sweet potato (ipomoea batatas) is among the most nutritious subtropical and tropical vegetables. it is also used in traditional medicine practices for type 2 diabetes mellitus. research in animal and human models suggests a possible role of sweet potato in glycaemic control. | 2012 | 22336861 |
| multiple biological functions of sporamin related to stress tolerance in sweet potato (ipomoea batatas lam). | the initial investigation of the nature of the proteins in the tuber of sweet potato (ipomoea batatas lam.) revealed a globulin-designated "ipomoein," which was reported by jones and gersdorff, (1931). later, "ipomoein" was renamed "sporamin" and was found to be a major storage protein that accounted for over 80% of the total protein in the tuberous root. to date, sporamin has been studied by a series of biochemical and molecular approaches. the first purification of sporamin into two major frac ... | 2013 | 22306516 |
| down-regulation of β-carotene hydroxylase increases β-carotene and total carotenoids enhancing salt stress tolerance in transgenic cultured cells of sweetpotato. | sweetpotato (ipomoea batatas lam.) is an important industrial crop and source of food that contains useful components, including antioxidants such as carotenoids. β-carotene hydroxylase (chy-β) is a key regulatory enzyme in the beta-beta-branch of carotenoid biosynthesis and it catalyzes hydroxylation into both β-carotene to β-cryptoxanthin and β-cryptoxanthin to zeaxanthin. to increase the β-carotene content of sweetpotato through the inhibition of further hydroxylation of β-carotene, the effec ... | 2012 | 22154923 |
| relationship between processing method and the glycemic indices of ten sweet potato (ipomoea batatas) cultivars commonly consumed in jamaica. | this study investigated the effect of different traditional cooking methods on glycemic index (gi) and glycemic response of ten sweet potato (ipomoea batatas) cultivars commonly eaten in jamaica. matured tubers were cooked by roasting, baking, frying, or boiling then immediately consumed by the ten nondiabetic test subjects (5 males and 5 females; mean age of 27 ± 2 years). the gi varied between 41 ± 5-93 ± 5 for the tubers studied. samples prepared by boiling had the lowest gi (41 ± 5-50 ± 3), ... | 2011 | 22132322 |
| oxidative comparison of emulsion systems from fish oil-based structured lipid versus physically blended lipid with purple-fleshed sweet potato (ipomoea batatas l.) extracts. | the effects of the purple-fleshed sweet potato extract (pfspe) on oxidation stabilities of a model oil-in-water emulsion prepared with enzymatically synthesized fish oil-soybean oil structured lipid (sl) versus physically blended lipid (pbl) without modification were evaluated. the anthocyanins in pfspe were analyzed and identified by hplc-ms. the fatty acid composition of sl was similar to that of pbl, except palmitic acid (1.48 in pbl and 9.61% in sl) and linoleic acid (62.47 in pbl and 49.58% ... | 2012 | 22117614 |
| scof-1-expressing transgenic sweetpotato plants show enhanced tolerance to low-temperature stress. | low-temperature stress represents one of the principal limitations affecting the distribution and productivity of many plant species, including crops such as sweetpotato. transgenic sweetpotato (ipomoea batatas l. cv. yulmi) plants expressing the soybean cold-inducible zinc finger protein (scof-1) under control of an oxidative stress-inducible peroxidase (swpa2) promoter (referred to as sf plants), were developed and evaluated for enhanced tolerance to low-temperature conditions. following 4 °c ... | 2011 | 22078381 |
| characterization and development of est-derived ssr markers in cultivated sweetpotato (ipomoea batatas). | currently there exists a limited availability of genetic marker resources in sweetpotato (ipomoea batatas), which is hindering genetic research in this species. it is necessary to develop more molecular markers for potential use in sweetpotato genetic research. with the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying ssr markers by data mining. | 2011 | 22011271 |
| meta-analysis of the effect of herbal supplement on glycemic control in type 2 diabetes. | a variety of herbs has been used in traditional medicine for the treatment of diabetes. however, evidence is limited regarding the efficacy of individual herbs for glycemic control. we performed a systematic review and meta-analysis to evaluate the effect of herbal supplement on glycemic control in type 2 diabetes. | 2011 | 21843614 |
| nitric oxide activates superoxide dismutase and ascorbate peroxidase to repress the cell death induced by wounding. | wounding caused by rain, wind, and pathogen may lead plants to onset defense response. previous studies indicated that mechanical wounding stimulates plants to generate nitric oxide (no) and hydrogen peroxide (h(2)o(2)). in this study, the functions of no and h(2)o(2) after wounding in sweet potato (ipomoea batatas cv. tainung 57) was further analyzed. mechanical wounding damaged cells and resulted in necrosis, but the presence of no donors or no scavenger might reduce or enhance the cell death ... | 2011 | 21833542 |
| occurrence of line, gypsy-like, and copia-like retrotransposons in the clonally propagated sweet potato (ipomoea batatas l.). | retrotransposons are a class of transposable elements that represent a major fraction of the repetitive dna of most eukaryotes. their abundance stems from their expansive replication strategies. we screened and isolated sequence fragments of long terminal repeat (ltr), gypsy-like reverse transcriptase (rt) and gypsy-like envelope (env) domains, and two partial sequences of non-ltr retrotransposons, long interspersed element (line), in the clonally propagated allohexaploid sweet potato (ipomoea b ... | 2011 | 21756057 |
| a sweetpotato srd1 promoter confers strong root-, taproot-, and tuber-specific expression in arabidopsis, carrot, and potato. | harvestable, starch-storing organs of plants, such as fleshy taproots and tubers, are important agronomic products that are also suitable target organs for use in the molecular farming of recombinant proteins due to their strong sink strength. to exploit a promoter directing strong expression restricted to these storage organs, we isolated the promoter region (3.0 kb) of srd1 from sweetpotato (ipomoea batatas cv. 'white star') and characterized its activity in transgenic arabidopsis, carrot, and ... | 2012 | 21660481 |
| molecular cloning and characterization of a flavonoid 3'-hydroxylase gene from purple-fleshed sweet potato (ipomoea batatas). | flavonoid 3'-hydroxylase (f3'h: ec 1.14.13.21) is an important enzyme which determines the hydroxylation pattern of anthocyanins. in this study, the full-length cdna and genomic dna of f3'h were isolated and characterized from the purple-fleshed sweet potato (ipomoea batatas). ibf3''h was 1,789 bp containing a 1,554 bp open reading frame (orf) encoding 518 amino acids. comparative and bioinformatic analysis revealed that ibf3'h was highly homologous with f3'hs from other plant species. conserved ... | 2012 | 21603861 |
| batatins iii-vi, glycolipid ester-type dimers from ipomoea batatas. | batatins iii-vi (1-4), glycolipid ester-type dimers, were isolated from the tuberous roots of sweet potato (ipomoea batatas) using recycle high performance liquid chromatography. their structures were characterized by means of several high-resolution nmr and mass spectrometry techniques. these compounds are the first examples of ester-type dimers which consist of two units of the heterotetrasaccharide operculinic acid c. each unit was esterified by a different amount and type of acid residues: ( ... | 2011 | 21453944 |
| relative susceptibility of two sweetpotato varieties to storage root damage by sweetpotato flea beetle (coleoptera: chrysomelidae) and wireworm (coleoptera: elateridae). | the feeding of soil dwelling insects on storage roots is one of the most serious management issues faced by sweetpotato, ipomoea batatas (l.) lam. (convolvulaceae), growers in the southern united states. field studies were conducted to evaluate the relative susceptibility of two commonly grown sweetpotato varieties to sweetpotato flea beetle, chaetocnema confinis crotch (coleoptera: chrysomelidae), and wireworms (coleoptera: elateridae, various species). the incidence and severity of sweetpotato ... | 2011 | 21404851 |
| de novo assembly and characterization of root transcriptome using illumina paired-end sequencing and development of cssr markers in sweet potato (ipomoea batatas). | the tuberous root of sweet potato is an important agricultural and biological organ. there are not sufficient transcriptomic and genomic data in public databases for understanding of the molecular mechanism underlying the tuberous root formation and development. thus, high throughput transcriptome sequencing is needed to generate enormous transcript sequences from sweet potato root for gene discovery and molecular marker development. | 2010 | 21182800 |
| sweetpotato late embryogenesis abundant 14 (iblea14) gene influences lignification and increases osmotic- and salt stress-tolerance of transgenic calli. | late embryogenesis abundant 14 (lea14) cdna was isolated from an est library prepared from dehydration-treated fibrous roots of sweetpotato (ipomoea batatas). quantitative rt-pcr revealed a variety of different iblea14 expression patterns under various abiotic stress conditions. iblea14 expression was strongly induced by dehydration, nacl and abscisic acid treatments in sweetpotato plants. transgenic sweetpotato non-embryogenic calli harboring iblea14 overexpression or rnai vectors under the con ... | 2011 | 21136074 |
| ipomotaosides a-d, resin glycosides from the aerial parts of ipomoea batatas and their inhibitory activity against cox-1 and cox-2. | four new resin glycosides, namely, ipomotaosides a-d (1-4), were isolated from the dried aerial parts of ipomoea batatas. the structures of 1-4 were elucidated by analysis of their spectroscopic data and by chemical derivatization and were tested for their anti-inflammatory activity against cyclooxygenase (cox)-1 and -2. | 2010 | 20961090 |
| sweet potato leaves: properties and synergistic interactions that promote health and prevent disease. | sweet potato (ipomoea batatas) leaves provide a dietary source of vitamins, minerals, antioxidants, dietary fiber, and essential fatty acids. bioactive compounds contained in this vegetable play a role in health promotion by improving immune function, reducing oxidative stress and free radical damage, reducing cardiovascular disease risk, and suppressing cancer cell growth. currently, sweet potato leaves are consumed primarily in the islands of the pacific ocean and in asian and african countrie ... | 2010 | 20883418 |
| survey of wireworms (coleoptera: elateridae) in north carolina sweetpotato fields and seasonal abundance of conoderus vespertinus. | adult and larval wireworm (coleoptera: elateridae) populations were surveyed in north carolina sweetpotato, ipomoea batatas (l.) lam., fields during 2005 and 2006 by using yellow sticky traps and larvalbaits. eight species of larvae and nine species of adult wireworms were identified. conoderus vespertinus (f.) was the most prevalent wireworm species, making up 65.9% of the larvae and 62.9% of the adults captured. adult c. vespertinus were most abundant in july, and larvae were smaller and more ... | 2010 | 20857736 |
| preparative isolation of anthocyanins from japanese purple sweet potato (ipomoea batatas l.) varieties by high-speed countercurrent chromatography. | purple-fleshed sweet potatoes (ipomoea batatas l.) contain a very complex anthocyanin profile due to the presence of several non-, mono-, and diacylated glucosides of cyanidin and peonidin. in this study, the anthocyanin composition of four japanese purple sweet potato cultivars (chiran murasaki, tanegashima murasaki, naka murasaki, and purple sweet) were investigated by hplc-dad and esi-msn analyses. the hplc chromatograms of the different cultivars show a remarkable variation of the two major ... | 2010 | 20731350 |
| differential responses of sweetpotato peroxidases to heavy metals. | oxidative stress is one of the major causes of damage in plants exposed to different types of environmental stress, including heavy metals. accumulation of heavy metals in plants can disrupt many cellular functions and plant growth. to assess the contribution of oxidative stress to heavy metal toxicity in plants, young sweetpotato plants (ipomoea batatas) were treated with increasing concentrations of cd, cu and zn, and grown in half murashige and skoog nutrient solution culture. plant growth wa ... | 2010 | 20638101 |
| heterogeneity of poly(a) sites in the granule-bound starch synthase i gene in sweet potato (ipomoea batatas (l.) lam.). | analysis of the cdnas of granule-bound starch synthase i (gbssi) in sweet potato indicated that six types of gbssi were expressed in the tuberous root, and that the poly(a) sites in gbssi were highly heterogeneous. several poly(a) sites were located within or downstream of the polymorphic ta repeat. the gbssi gene has a 23-nucleotide a-rich sequence in the 3' untranslated region, and we believe that the main near-upstream elements of the poly(a) signal are included in this sequence. | 2010 | 20208377 |
| [fish analysis of chromosomes of sweet potato(ipomoea batatas cv.xushu no.18)]. | in order to understand the chromosome structure of sweet potato (ipomoea batatas cv. xushu 18), molecular cytogenetic analyses were carried out on i. batatas. by using 45s rdna fluorescence in situ hybridization (45s rdna-fish), self genomic in situ hybridization (self-gish), and silver staining techniques. twelve, sixteen, and eighteen regions were silver stained in the interphase nucleus of i. batatas. the results of fish analysis demonstrated 16 or 18 signals with different intensity on chrom ... | 2010 | 20176563 |
| srd1 is involved in the auxin-mediated initial thickening growth of storage root by enhancing proliferation of metaxylem and cambium cells in sweetpotato (ipomoea batatas). | a sweetpotato (ipomoea batatas cv. 'jinhongmi') mads-box protein cdna (srd1) has been isolated from an early stage storage root cdna library. the role of the srd1 gene in the formation of the storage root in sweetpotato was investigated by an expression pattern analysis and characterization of srd1-overexpressing (ox) transgenic sweetpotato plants. transcripts of srd1 were detected only in root tissues, with the fibrous root having low levels of the transcript and the young storage root showing ... | 2010 | 20150515 |
| in vitro bioaccessibility of beta-carotene in orange fleshed sweet potato (ipomoea batatas, lam.). | substitution of white with orange fleshed varieties of sweet potatoes (ofsp) was recently shown to alleviate vitamin a deficiency in children in africa. however, the relationship between beta-carotene (bc) content of different cultivars of ofsp and its bioavailability is unknown. here, we used the three phase (oral, gastric and small intestinal) in vitro digestion procedure to examine the bioaccessibility of bc from eight cultivars of boiled ofsp. all-trans bc (all-e-bc) was the only isomer of b ... | 2009 | 19919124 |
| [study on the chemical constituents of the leaves of ipomoea batatas]. | to study the chemical constituents of the leaves of ipomoea batatas. | 2009 | 19764329 |
| peptide signals for plant defense display a more universal role. | hydroxyproline-rich systemins (hypsys) are small defense signaling glycopeptides found within the solanaceae family that until recently were thought to only induce defense genes to herbivore attack. the glycopeptides are processed from larger proproteins with up to 3 different glycopeptides being processed out of a single precursor protein. a conserved central hydroxyproline motif within each hypsys is the site of pentose sugar attachment. recently, it was found that in petunia hybrida, these de ... | 2008 | 19704502 |
| characterization of full-length enriched expressed sequence tags of dehydration-treated white fibrous roots of sweetpotato. | sweetpotato (ipomoea batatas (l). lam.) is relatively tolerant to unfavorable growth conditions such as drought, yet has not been exploited to provide a better understanding of the molecular basis of drought stress tolerance. we obtained 983 high-quality expressed sequence tags of 100 bp or longer (average length of 700 bp) from cdna libraries of detached white fibrous root tissues by subjecting them to dehydration for 6 h. the 431 cdnas were each assigned a function by alignment using the blast ... | 2009 | 19470240 |
| cloning, expression, and purification of a functional glutathione reductase from sweet potato (ipomoea batatas [l.] lam): kinetic studies and characterization. | a cdna encoding a putative glutathione reductase (gr) was cloned from sweet potato (ib). the deduced protein showed high level of sequence homology with grs from other plants (79-38%). a three-dimensional (3-d) homology structure was created. the active site cys residues are conserved in all reported gr. functional ibgr was overexpressed and purified. the purified enzyme showed an active monomeric form on a 10% native polyacrylamide gel electrophoresis (page). the monomeric nature of the enzyme ... | 2009 | 19358534 |
| cloning and characterization of the rubisco activase gene from ipomoea batatas (l.) lam. | a full-length cdna of rubisco activase (ibrcai) was cloned from sweet potato (ipomoea batatas (l.) lam) using rapid-amplification of cdna ends (race). ibrcai contains a 1,347 bp open reading frame encoding a protein of 439 amino acids. the sequence alignment of multiple rubisco activase genes from sweet potato and other plants showed high homology at two previously described atp-binding sites. western blot analysis indicated that there are two rubisco activase proteins in sweet potato. expressio ... | 2010 | 19296237 |
| an improved method of isolation of high quality total rna from purple-fleshed sweet potato, ipomoea batatas (l.) lam. | to isolate high quality total rna from plants of purple-fleshed sweet potato (ipomoea batatas l. lam) is difficult because these plants contain high levels of carbohydrates and pigment compounds. in this study, we developed a quick and reliable method for total rna extraction. using this extraction protocol, which was derived from the traditional ctab method with modification, we obtained high quality total rna from all the tested tissues including leaf blade, petiole, stem, fibrous root, thick ... | 2009 | 19291573 |
| [studies on chemical constituents from ipomoea batatas]. | to investigate the chemical constituents of ipomoea batatas. | 2008 | 19230398 |
| anthocyanidin-containing compounds occur in the periderm cell walls of the storage roots of sweet potato (ipomoea batatas). | anthocyanins, which are o-glycosylated derivatives of anthocyanidins, are responsible for the red, blue and purple coloration of many organs of angiosperms where they have previously been reported to occur in vacuoles and cytoplasm. however, bright-field microscopy of sections of the storage roots of the sweet potato (ipomoea batatas) clone 99n1/222, which contains high concentrations of anthocyanins, showed that the walls of the periderm cork cells (skin), but not those of the adjacent parenchy ... | 2009 | 19201049 |
| novel acylated lipo-oligosaccharides from the tubers of ipomoea batatas. | nine new lipo-oligosaccharides, batatosides h-p, were isolated from the tubers of ipomoea batatas (convolvulaceae). spectral and chemical methods allowed to characterize them as tetra- or penta-saccharides that form a macrolactone with the aglycone, (11s)-hydroxyhexadecanoic acid (jalapinolic acid), the absolute configuration of which was established by mosher's method. batatosides l and o showed a weak inhibitory effect on the growth of hep-2 cells, while the others proved to be inactive. | 2009 | 19159869 |
| three new pentasaccharide resin glycosides from the roots of sweet potato (ipomoea batatas). | three new pentasaccharide resin glycosides, batatosides iii-v (1-3), were isolated from the roots of sweet potato (ipomoea batatas). saponification of the crude resin glycoside mixture yielded substituents and simonic acid b. the structures of the isolated compounds (1-3) were established through spectroscopic analyses, including high field nmr spectroscopy and hr-esi-ms, and chemical correlation. the major characteristics of 3 are the presence of three different substituents, especially the sub ... | 2008 | 19043237 |
| the resin glycosides from the sweet potato (ipomoea batatas l. lam.). | four new and two known ether-soluble resin glycosides were isolated from popular sweet potato (the roots of ipomoea batatas l. lam., kokei 14 go, convolvulaceae) in japan. unlike ester-type dimers, batatins i and ii, obtained from other sweet potato (ipomoea batabas var. batatas), the glycosides were tetra or pentasaccharide monomers in which the sugar moieties are partially acylated by organic acids and combine with the aglycone, jalapinolic acid, to form a macrocyclic ester. | 2008 | 18981615 |
| batatinosides ii-vi, acylated lipooligosaccharides from the resin glycosides of sweet potato. | sweet potato ( ipomoea batatas) belongs to the convolvulaceae (morning glory family) and is native to mexico and central america. its edible tuberous roots have been much appreciated since pre-hispanic times in mesoamerica and now play an important role as a basic diet staple and a medicinal plant worldwide. the hexane-soluble extract from roots, through preparative-scale recycling hplc, yielded five new lipophilic oligosaccharides of jalapinolic acid, batatinosides ii-vi ( 1- 5), as well as the ... | 2008 | 18816059 |
| influence of soil temperature, rainfall, and planting and harvest dates on chaetocnema confinis (coleoptera: chrysomelidae) damage to sweetpotato roots. | a study was carried out in 10 counties of north carolina from 2004 to 2006 to determine the effect of planting and harvest times on flea beetle, chaetocnema confinis crotch (coleoptera: chrysomelidae), damage to sweetpotato, ipomoea batatas (l.), storage roots. planting and harvesting of sweetpotatoes later in the season resulted in less damage than early planting and harvesting. regression analysis was done to study the relationship of weather parameters with the flea beetle damage. weather par ... | 2008 | 18767733 |
| sweet potato [ipomoea batatas (l.) lam.] cultivated as tuber or leafy vegetable supplier as affected by elevated tropospheric ozone. | sweet potato cultivars respond differently to elevated tropospheric ozone concentrations of ca. 130 mug m (-3), 8 h a day for 4 weeks, which affects their selection for cultivation. in the first cultivar presented here, an adequate leafy vegetable supplier, the ozone load resulted in a shift of biomass to maintain the canopy at the expense of tuber development. starch content of leaves was reduced, indicating an impairment of quality, but carotenoid content remained stable. the second cultivar m ... | 2008 | 18593180 |
| flow-batch technique for the simultaneous enzymatic determination of levodopa and carbidopa in pharmaceuticals using pls and successive projections algorithm. | an enzymatic flow-batch system with spectrophotometric detection was developed for simultaneous determination of levodopa [(s)-2 amino-3-(3,4-dihydroxyphenyl)propionic acid] and carbidopa [(s)-3-(3,4-dihydroxyphenyl)-2-hydrazino-2-methylpropionic acid] in pharmaceutical preparations. the data were analysed by univariate method, partial least squares (pls) and a novel variable selection for multiple lineal regression (mlr), the successive projections algorithm (spa). the enzyme polyphenol oxidase ... | 2008 | 18585168 |
| effects of prolonged restriction in water supply on photosynthesis, shoot development and storage root yield in sweet potato. | besides the paucity of information on the effects of drought stress on photosynthesis and yield in sweet potato [ipomoea batatas (l.) lam.], available reports are also contradictory. the aim of this study was to shed light on the effects of long-term restricted water supply on shoot development, photosynthesis and storage root yield in field-grown sweet potato. experiments were conducted under a rainout shelter where effects of restricted water supply were assessed in two varieties (resisto and ... | 2008 | 18494734 |
| dehydroascorbate reductase cdna from sweet potato (ipomoea batatas [l.] lam): expression, enzyme properties, and kinetic studies. | a cdna encoding a putative dehydroascorbate reductase (dhar) was cloned from sweet potato. the deduced protein showed a high level of sequence homology with dhars from other plants (67 to approximately 81%). functional sweet potato dhar was overexpressed and purified. the purified enzyme showed an active monomeric form on a 12% native page. the protein's half-life of deactivation at 50 degrees c was 10.1 min, and its thermal inactivation rate constant k(d) was 6.4 x 10(-2) min(-1). the enzyme wa ... | 2008 | 18444663 |
| influence of microgravity environment on root growth, soluble sugars, and starch concentration of sweetpotato stem cuttings. | because sweetpotato [ipomoea batatas (l.) lam.] stem cuttings regenerate very easily and quickly, a study of their early growth and development in microgravity could be useful to an understanding of morphological changes that might occur under such conditions for crops that are propagated vegetatively. an experiment was conducted aboard a u.s. space shuttle to investigate the impact of microgravity on root growth, distribution of amyloplasts in the root cells, and on the concentration of soluble ... | 2008 | 20186286 |
| molecular cloning of peroxidase cdnas from dehydration-treated fibrous roots of sweetpotato and their differential expression in response to stress. | three peroxidase (pod) cdnas were isolated from dehydration-treated fibrous roots of sweetpotato (ipomoea batatas) plant via the screening of a cdna library, and their expressions were assessed to characterize functions of each pod in relation to environmental stress. three pods were divided into two groups, designated the basic pods (swpb4, swpb5) and the anionic pods (swpa7), on the basis of the pi values of mature proteins. fluorescence microscope analysis indicated that three pods are secret ... | 2008 | 18377732 |
| pentasaccharide glycosides from the tubers of sweet potato (ipomoea batatas). | sweet potato ( ipomoea batatas) has been used as food and herb in many countries. in this research on the active constituents of sweet potato, nine compounds were isolated and identified, including seven new resin glycosides, batatosides a-g (1- 7), along with two known compounds, batatinoside i ( 8) and simonin iv ( 9). the structures of 1- 9 have been established by a combination of spectroscopic and chemical methods. the major characteristics of the new compounds are the presence of three dif ... | 2008 | 18341282 |
| ether-soluble resin glycosides from the roots of ipomoea batatas. | two new resin glycosides, batataosides i (1) and ii (2), and five known compounds, friedelin (3), scopoletin (4), octadecyl caffeate (5), beta-sistosterol (6) and daucosterol (7), were isolated from the roots of ipomoea batatas. their structures have been determined based on the chemical and spectral data. batataosides i and ii have novel structures because the core simonic acid b was esterised with cinnamic acid for the first time, and three different substituent esterification groups in one re ... | 2013 | 18335340 |
| six peptide wound signals derived from a single precursor protein in ipomoea batatas leaves activate the expression of the defense gene sporamin. | a mixture of three homologous bioactive hydroxyproline-rich glycopeptides (hypsys peptides) of 18 amino acids in length, differing only at two residues, was isolated from leaves of ipomoea batatas, the common sweet potato. one of the peptides represented over 95% of the isolated isopeptides, which, at 2.5 nm concentration, induced the expression of sporamin, a major defense protein of i. batatas. the sequence of the major isoform was used to synthesize a primer that identified a cdna encoding a ... | 2008 | 18299332 |
| effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (ipomoea batatas lam.). | lateral buds of six cultivars of sweet potato were induced to form embryogenic callus in a culture medium solidified with two types of gelling agents, agar or gelrite, and supplemented with various concentrations of auxins, 2,4-d, 2,4,5-t and picloram. of the six cultivars screened, only three gave an embryogenic response. best results with an average of 3.53% embryogenic response were obtained with the medium solidified with agar, while in gelrite only 0.45% of lateral buds gave rise to embryog ... | 2008 | 18280985 |
| expression of class i knotted1-like homeobox genes in the storage roots of sweetpotato (ipomoea batatas). | as a first step in clarifying the involvement of class i knotted1-like homeobox (knoxi) genes in the storage root development of sweetpotato (ipomoea batatas), we isolated three knoxi genes, named ibkn1, ibkn2 and ibkn3, expressed in the storage roots. phylogenetic analysis showed that ibkn1 was homologous to the shoot meristemless (stm) gene of arabidopsis, while ibkn2 and ibkn3 were homologous to the brevipedicellus (bp) gene. of these, expression of ibkn1 and ibkn2 were upregulated in develop ... | 2008 | 18242774 |
| glycosylation of hesperetin by plant cell cultures. | the biotransformation of hesperetin by cultured cells of ipomoea batatas and eucalyptus perriniana was investigated. three glycosides, hesperetin 3'-o-beta-d-glucopyranoside (33 microg/g fr. wt of cells), hesperetin 3',7-o-beta-d-diglucopyranoside (217 microg/g fr. wt of cells), and hesperetin 7-o-[6-o-(beta-d-glucopyranosyl)]-beta-d-glucopyranoside (beta-gentiobioside, 22 microg/g fr. wt of cells), together with three hitherto known glycosides, hesperetin 5-o-beta-d-glucopyranoside (23 microg/g ... | 2008 | 18160083 |
| changes in polyphenolic content and radical-scavenging activity of sweet potato (ipomoea batatas l.) during storage at optimal and low temperatures. | polyphenolic content and radical-scavenging activities (rsa) of four sweet potato (ipomoea batatas l.) cultivars were characterized after storage at optimal (15 degrees c) or low temperature (5 degrees c) for 0, 13, 26, and 37 days. the polyphenolic content increased during storage in three cultivars but not in 'murasakimasari'. the change in 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity (dpph-rsa) correlated very well with polyphenolic content. the increases in polyphenolics and the ... | 2007 | 18038989 |
| molecular characterization of a cdna encoding dre-binding transcription factor from dehydration-treated fibrous roots of sweetpotato. | a new dehydration responsive element-binding (dreb) protein gene encoding for an ap2/erebp-type transcription factor was isolated by screening of the cdna library for dehydration-treated fibrous roots of sweetpotato (ipomoea batatas). its cdna (referred to as swdreb1) fragment of 1206bp was sequenced from, which a 257 amino acid residue protein was deduced with a predicted molecular weight of 28.17kda. a search of the protein blast database revealed that this protein can be classified as a typic ... | 2008 | 18006323 |
| methodology optimization for quantification of total phenolics and individual phenolic acids in sweetpotato (ipomoea batatas l.) roots. | phenolic acids are one of the several classes of naturally occurring antioxidant compounds found in sweetpotatoes. simplified, robust, and rapid methodologies were optimized to quantify total and individual phenolic acids in sweetpotato roots. total phenolic acid content was quantified spectrophotometrically using both folin-denis and folin-ciocalteu reagents. the folin-ciocalteu reagent gave an overestimation of total phenolic acids due to the absorbance of interfering compounds (that is, reduc ... | 2007 | 17995640 |
| novel galactolipids from the leaves of ipomoea batatas l.: characterization by liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight tandem mass spectrometry. | sixteen novel and ten known galactolipids have been isolated and characterized from the leaves of ipomoea batatas l. (sweet potato) using an analytical method based on high-performance liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight tandem mass spectrometry. using this technique, the structures and regiochemistries of the fatty acyl groups and the positions of the double bonds on the acyl chains were determined. sugar moieties were identified by analysis of o ... | 2007 | 17988089 |
| calcium influxes and mitogen-activated protein kinase kinase activation mediate ethylene inducing ipomoelin gene expression in sweet potato. | the ipomoelin gene (ipo) was identified to be a wound-inducible gene from ipomoea batatas, and its expression was stimulated by methyl jasmonate (meja) and hydrogen peroxide. ipo protein was also characterized as a defence-related protein, and it is also a carbohydrate-binding protein. in this study, the expression of ipo was used as a molecular probe to study the effects of ca2+ on the signal transduction of ethylene. a confocal microscope monitored the ca2+ within cells, and northern blotting ... | 2008 | 17971062 |
| differential expression of 10 sweetpotato peroxidases in response to sulfur dioxide, ozone, and ultraviolet radiation. | secretory class iii plant peroxidase (pod, ec 1.11.1.7) is believed to function in diverse physiological processes, including responses to various environmental stresses. to understand the function of each pod in terms of air pollutants and uv radiation, changes in pod activity and expression of 10 pod genes isolated from cell cultures of sweetpotato (ipomoea batatas) were investigated in the leaves of sweetpotato after treatment with sulfur dioxide (so(2) 500ppb, 8h/day for 5 days), ozone (o(3) ... | 2007 | 17870589 |
| improved metabolic control by ipomoea batatas (caiapo) is associated with increased adiponectin and decreased fibrinogen levels in type 2 diabetic subjects. | the extract of the white-skinned sweet potato ipomoea batatas (caiapo) has been shown to ameliorate glucose control by improving insulin sensitivity in patients with type 2 diabetes mellitus (t2dm). the present study was designed to further evaluate its mode of action on insulin sensitivity over an extended period of time as well as the effects on fibrinogen and other markers of low-grade inflammation. | 2008 | 17645559 |
| batatins i and ii, ester-type dimers of acylated pentasaccharides from the resin glycosides of sweet potato. | batatins i (1) and ii (2), two ester-type dimers of acylated pentasaccharides, have been isolated by recycling hplc from the hexane-soluble extract of sweet potato (ipomoea batatas var. batatas). the structures were elucidated by a combination of high-resolution nmr spectroscopy and mass spectrometry. complete analysis and unambiguous assignments of the 1h and 13c nmr spectra of 1 and 2 were achieved by 2d shift correlation measurements. the glycosidic acid forming each branched pentasaccharide ... | 2007 | 17488129 |
| carotenoids with a 5,6-dihydro-5,6-dihydroxy-beta-end group, from yellow sweet potato "benimasari", ipomoea batatas lam. | a series of carotenoids with a 5,6-dihydro-5,6-dihydroxy-beta-end group, named ipomoeaxanthins a (1), b (2), c1 (3) and c2 (4) were isolated from the flesh of yellow sweet potato "benimasari", ipomoea batatas lam. their structures were determined to be (5r,6s,3'r)-5,6-dihydro-beta,beta-carotene-5,6,3'-triol (1), (5r,6s,5'r,6's)-5,6,5',6'-tetrahydro-beta,beta-carotene-5,6,5'6'-tetrol (2), (5r,6s,5'r,8'r)-5',8'-epoxy-5,6,5',8'-tetrahydro-beta,beta-carotene-5,6-diol (3), and (5r,6s,5'r,8's)-5',8'-e ... | 2007 | 17466348 |
| enzymatic reverse fia method for total phenols determination in urine samples. | a reverse flow injection spectrophotometric enzymatic method is proposed to quantify total phenols in urine samples. the polyphenol oxidase (ppo; ec 1.14.18.1) obtained as a crude extract from sweet potato root (ipomoea batatas) was used as enzymatic catalyze. the detection limit, the sample throughput and relative standard deviation were 7.7mgl(-1) of total phenols, 49h(-1) and 0.9%, respectively. the method was applied to real samples and a recovery study was carried out in order to its valida ... | 2007 | 19071485 |
| triterpene saponin hemi-biosynthesis of a leaf beetle's (platyphora kollari) defensive secretion. | the adults of the leaf beetle platyphora kollari (chrysomelidae) are able to metabolise the oleanane triterpene beta-amyrin (1) into the glycoside 3-o-beta-d: -glucopyranosyl-(1-->4)-beta-d: -glucuronopyranosyl-hederagenin (2) that is stored in their defensive glands. the aim of this study was to test the hypothesis that oleanolic acid (3) is an intermediate in the conversion of 1 into 2 and to check whether the sequestration of pentacyclic triterpenes is selective in favour of beta-amyrin (1). ... | 2007 | 17354008 |
| growth inhibition and induction of apoptosis in nb4 promyelocytic leukemia cells by trypsin inhibitor from sweet potato storage roots. | the objective of this study was to investigate the antiproliferative effect and the mechanism of trypsin inhibitor (ti) from sweet potato [ipomoea batatas (l.) lam. 'tainong 57'] storage roots on nb4 promyelocytic leukemia cells. the results showed that ti inhibited cellular growth of nb4 promyelocytic leukemia cells in a time-dependent and dose-dependent manner, and treatment for 72 h induced a marked inhibition of cellular growth, showing an ic50 of 57.1 +/- 8.26 microg/ml. ti caused cell cycl ... | 2007 | 17328557 |
| analysis of sphingolipids in potatoes (solanum tuberosum l.) and sweet potatoes (ipomoea batatas (l.) lam.) by reversed phase high-performance liquid chromatography electrospray ionization tandem mass spectrometry (hplc-esi-ms/ms). | ceramides and glucocerebrosides of potatoes (solanum tuberosum l.) and sweet potatoes (ipomoea batatas (l.) lam.) were analyzed using rp-hplc-esi-ms/ms. ceramides and glucocerebrosides containing the three different long-chain bases 4,8-sphingadienine (d18:2(delta4,delta8)), 4-hydroxy-8-sphingenine (t18:1(delta8)), and 8-sphingenine (d18:1(delta8)) acylated to saturated and unsaturated hydroxy- and nonhydroxy fatty acids with 16-26 carbon atoms were detected. for ceramides and glucocerebrosides ... | 2006 | 17103377 |
| new polyphenol derivative in ipomoea batatas tubers and its antioxidant activity. | four different polyphenolic compounds were isolated by chromatographic methods from methanolic and hydromethanolic extracts of ipomoea batatas tuber flour. on the basis of uv, mass, and nmr analysis procedures, the structure of the isolated compounds were determined as 4,5-di-o-caffeoyldaucic acid (1), 4-o-caffeoylquinic acid (2), 3,5-di-o-caffeoylquinic acid (3), and 1,3-di-o-caffeoylquinic acid (4). to the best of our knowledge, this is the first report of isolation and characterization of com ... | 2006 | 17090114 |
| luminol-hydrogen peroxide chemiluminescence produced by sweet potato peroxidase. | anionic sweet potato peroxidase (spp; ipomoea batatas) was shown to efficiently catalyse luminol oxidation by hydrogen peroxide, forming a long-term chemiluminescence (cl) signal. like other anionic plant peroxidases, spp is able to catalyse this enzymatic reaction efficiently in the absence of any enhancer. maximum intensity produced in spp-catalysed oxidation of luminol was detected at ph 7.8-7.9 to be lower than that characteristic of other peroxidases (8.4-8.6). varying the concentrations of ... | 2013 | 17089355 |
| [drought tolerance of transgenic sweet potato expressing both cu/zn superoxide dismutase and ascorbate peroxidase]. | two strains, cu/zn sod and apx gene transferred sweet potato (ts) and non-transgenic sweet potato (ipomoea batatas l.) (nt), were used as experimental materials to study the drought tolerance under three different degrees of water stress: 0, -0.44 mpa, -0.78 mpa. the results showed that activities of cu/zn sod and apx increased under -0.44 mpa and decreased under -0.78 mpa (fig. 2), p(n), g(s) and leaf water content decreased, c(i) increased, then decreased under water stress (fig. 6), but under ... | 2006 | 16957397 |
| the origin and evolution of sweet potato (ipomoea batatas lam.) and its wild relatives through the cytogenetic approaches. | the distribution and organization of 5s and 18s-5.8s-26s (18s) rdna were studied in 10 varieties of hexaploid ipomoea batatas, five accessions of tetraploid ipomeoa trifida, and six related species (five diploids, i. trifida, i. triloba, i. tiliacea, i. leucantha and i. setosa and one tetraploid, i. tabascana), by using fluorescence in situ hybridization (fish). the fish data obtained indicated that polyploidization was followed by decrease in the number of 18s rdna loci in higher ploidy level a ... | 2006 | 22980213 |
| seven new aminoacyl sugars in ipomoea batatas. | an analysis of the polar extracts from sweet potato ipomoea batatas (convolvulaceae) led to the isolation of seven unknown aminoacyl sugars. on the basis of 1d, 2d nmr, and mass spectrometry data, the structures of the compounds were elucidated as: beta-d-fructofuranosyl-(2 --> 1)-alpha-d-[2-o-valyl]-glucopyranoside (1), beta-d-fructofuranosyl-(2 --> 1)-alpha-d-[2-o-tyrosyl]-glucopyranoside (2), beta-d-fructofuranosyl-(2 --> 1)-alpha-d-[2-o-threonyl]-glucopyranoside (3), beta-d-fructofuranosyl-( ... | 2006 | 16881721 |
| starch-branching enzyme i gene (ibsbei) from sweet potato (ipomoea batatas); molecular cloning and expression analysis. | the cdna of the starch-branching enzyme i gene (ibsbei) in the sweet potato (ipomoea batatas) has been cloned and sequenced. the ibsbei amino acid sequence was 81% identical to that of potato stsbei. dna gel-blot analyses demonstrated that at least two copies of ibsbei are present in the sweet potato genome. ibsbei was strongly expressed in tuberous roots. transcript levels in the roots of single leaf cuttings were extremely low during the first 15-40 d after planting and continuously increased ... | 2006 | 16802100 |
| molecular cloning and characterization of a granulin-containing cysteine protease spcp3 from sweet potato (ipomoea batatas) senescent leaves. | granulins are a family of evolutionarily ancient proteins that are involved in regulating cell growth and division in animals. in this report a full-length cdna, spcp3, was isolated from senescent leaves of sweet potato (ipomoea batatas). spcp3 contains 1389 nucleotides (462 amino acids) in its open reading frame, and exhibits high amino acid sequence homologies (ca. 64-73.6%) with several plant granulin-containing cysteine proteases, including potato, tomato, soybean, kidney bean, pea, maize, r ... | 2006 | 16777534 |
| changes in caffeic acid derivatives in sweet potato (ipomoea batatas l.) during cooking and processing. | there was an obvious decrease in caffeic acid derivatives during the boiling of cube-shaped blocks of sweet potatoes. they also decreased in a mixture of freeze-dried sweet-potato powder and water maintained at room temperature. ascorbic acid prevented the decrease, supporting the occurrence of an enzyme reaction with polyphenol oxidase (ppo). 5-o-caffeoylquinic acid (5-cqa, "3-o-caffeoylquinic acid" as a trivial name) and 3,5-di-o-caffeoylquinic acid (3,5-cqa), major phenolic compounds of sweet ... | 2006 | 16428835 |
| rapid and reliable method of extracting dna and rna from sweetpotato, ipomoea batatas (l). lam. | a quick, simple and reliable method of extracting dna from sweetpotato (ipomoea batatas (l.) lam.) has been developed. the method was applied successfully for extraction of total dna from leaves and total rna from leaves and various tissues. the yield of dna extracted by this procedure was high (about 1 mg/g leaf tissue). the extracted dna was completely digested by restriction endonucleases indicating the absence of common contaminating compounds. the absorbancy ratios of a260/a230 and a260/a28 ... | 2005 | 16328977 |
| regioselective acylation of flavonoid glucoside with aromatic acid by an enzymatic reaction system from cultured cells of ipomoea batatas. | the regioselective acylation of naturally occurring plant pigments with aromatic acids such as caffeic acid and p-coumaric acid was examined by an enzymatic reaction system from cultured cells of ipomoea batatas. isoquercitrin, a flavonoid glucoside, was converted to isoquercitrin 6''-o-caffeate by the enzymatic system. chrysanthemin, an anthocyanin, was also acylated to the corresponding caffeate ester. this enzymatic method should prove useful for the synthesis of acylated flavonoid glucosides ... | 2000 | 16232870 |
| the regulation of rubisco activity in response to variation in temperature and atmospheric co2 partial pressure in sweet potato. | the temperature response of net co(2) assimilation rate (a), the rate of whole-chain electron transport, the activity and activation state of rubisco, and the pool sizes of ribulose-1,5-bisphosphate (rubp) and 3-phosphoglyceric acid (pga) were assessed in sweet potato (ipomoea batatas) grown under greenhouse conditions. above the thermal optimum of photosynthesis, the activation state of rubisco declined with increasing temperature. doubling co(2) above 370 mubar further reduced the activation s ... | 2005 | 16183840 |
| probing anthocyanin profiles in purple sweet potato cell line (ipomoea batatas l. cv. ayamurasaki) by high-performance liquid chromatography and electrospray ionization tandem mass spectrometry. | a purple line cell line (pl) generated from the storage root of purple-fleshed sweet potato (ipomoea batatas l.) cv. ayamurasaki produces a complex mixture of anthocyanins, and seven major anthocyanins have been isolated and identified to date. all these anthocyanins are exclusively cyanidin or peonidin 3-sophoroside-5-glucosides and their acylated derivatives. high-performance liquid chromatography (hplc) coupled to photodiode array (pda) detection and electrospray ionization tandem mass spectr ... | 2005 | 16076141 |
| [study on flavonoids from leaf of ipomoea batatas]. | to investigate the flavonoid constituents of ipomoea batatas. | 2005 | 16011096 |
| [alkaloids of the hairy roots of ipomoea batatas lam]. | to study the alkaloid constituents of the hairy roots of ipomoea batatas lam. | 2004 | 15700823 |