the influence of cooked kidney beans (phaseolus vulgaris) on intestinal cell turnover and faecal nitrogen excretion in the rat. | male wistar rats were fed on semi-synthetic diets containing cooked white kidney beans (phaseolus vulgaris) or equivalent levels of protein and carbohydrate. no change was observed in over-all nitrogen balance in animals fed on the bean diet, but there was a two- to three-fold increase in their faecal excretion, compared with control rats. this was compensated by a decrease in urinary-n excretion. homogenized small intestinal mucosa, prepared from bean-fed animals, showed a 28% increase in prote ... | 1983 | 6860620 |
toxic effects of kintoki bean (phaseolus vulgaris) lectin on cultured animal cells. | effects of a toxic lectin from kintoki beans (phaseolus vulgaris, cultivar, kintoki) on four types of animal cells were investigated. the cells used in this study were mouse l 929, human hela s3, and tendon and liver cells from chick embryo. the kintoki bean lectin agglutinated these cells not only in suspension but also in monolayer, resulting in the marked growth inhibition of these cells. the incorporations of 3h-thymidine and 3h-leucine into trichloroacetic acid insoluble fraction of the cel ... | 1983 | 6864349 |
anatomy of fetal rabbit gonads and the sexing of fetal rabbits. | fetal sex can be accurately and rapidly assessed in fetuses of 24 days gestation to term (31 days) by examination of the gonads. at 24 days both testis and ovary are a few millimeters below the kidney. with advancing gestation the testis descends markedly so that by 28 days it lies in the lowest region of the retroperitoneum. the ovary descends only slightly, never reaching a position more than halfway between the kidney and the bottom of the retroperitoneal space. the testis is larger, thicker, ... | 1983 | 6865323 |
effects of extracts from bean (phaseolus vulgaris) and field bean (vicia faba) varieties on intestinal d-glucose transport in rat in vivo. | | 1983 | 6865340 |
interaction of the brush-border hydrolases of the human small intestine with lectins. | the role of human intestinal brush-border membrane hydrolases in lectin binding to the apical region of the enterocyte was investigated. the crossed-immunoelectrophoresis method was used to identify six hydrolases before and after interaction with nine lectins. three lectins (phaseolus vulgaris, ulex europeus, and ricinus communis 120 agglutinins) bound the hydrolases of 5 different subjects. helix pomatia agglutinin interacted with only 1 subject. | 1983 | 6884583 |
synergistic effects of lectins in the interaction of thrombin with human platelets. | several lectins have been studied for their effects on the interaction of thrombin with human platelets. wheat germ agglutinin, concanavalin a and ricinus communis lectin increased the number of high affinity sites for diisopropylphosphothrombin on washed platelets from 3000 to about 12 000 but the binding affinities were unchanged (kd approx 4 nm). two other lectins, lens culinaris and bandieria simplicifolia, were without effect. (2) using formalinized platelets to avoid possible complications ... | 1981 | 6894391 |
reversible inhibition of the in vitro coagulation of human plasma by lectins. | wheat germ agglutinin (wga) and concanavalin a (con-a) (also red kidney bean agglutinin, pha) have been found to be inhibitors of plasma clotting in vitro. at 40 micrograms/ml and 250 micrograms/ml (4.4 microm and 10 microm in carbohydrate binding sites, final concentrations) respectively, wga and con-a are able to double the activated partial thromboplastin time of normal human control plasma. their inhibitory effect is due to their capacity to interact with the carbohydrate portion of blood cl ... | 1982 | 6897462 |
does mixing pancreatic enzyme microspheres (pancrease) with food damage the enteric coating? | supplemental pancreatic enzymes are required for the treatment of malabsorption associated with cystic fibrosis. it is a general practice for enzymes to be sprinkled on, or administered in, food given to infants or children who require pancreatic enzyme replacement. we tested the effect of in vitro incubation of enteric-coated pancreatic enzyme microspheres with infant foods on total recoverable enzyme activity and on the enteric coating. incubation of enzyme beads with foods with ph greater tha ... | 1982 | 6926537 |
a case-control study of large bowel cancer in japan. | this investigation of diet and other environmental factors reports on 588 patients with colorectal cancer and 1,176 hospitalized controls in three prefectures of japan. weak (not statistically significant) positive effects were found for social class and urbanization. the significant association of colorectal cancer with consumption of beef, string beans, or starches previously described for hawaiian japanese were not reproduced here. an association with hakusal (cabbage) agreed with other repor ... | 1980 | 6928043 |
mitogenic leukoagglutinin from phaseolus vulgaris binds to a pentasaccharide unit in n-acetyllactosamine-type glycoprotein glycans. | the carbohydrate binding specificity of leukoagglutinin (la; phaseolus vulgaris isolectin l4) was studied by using quantitative precipitation and precipitation-inhibition. a series of purified glycopeptides and synthetic oligosaccharides were used as inhibitors. the minimal structural unit required for la binding was the disaccharide glcnac(1 leads to beta 2)man. additions for this basic unit of different sugar residues gave a positive or negative contribution to binding. the most complementary ... | 1982 | 6951200 |
the effect of diets containing field beans of high or low polyphenolic content on the activity of digestive enzymes in the intestines of rats. | | 1980 | 6966014 |
lectin receptors as markers for trypanosoma cruzi. developmental stages and a study of the interaction of wheat germ agglutinin with sialic acid residues on epimastigote cells. | trypanosoma cruzi at various stages of maturation and differentiation have been isolated by conventional cellular fractionation procedures and characterized by cell surface markers using 30 highly purified lectins encompassing all known sugar specificities. cell surface carbohydrates of the various t. cruzi stages were analyzed by agglutination and lectin-binding assays. specific receptors for wheat germ agglutinin (wga), helix pomatia, sophora japonica, and bandeiraea simplicifolia lectin ii we ... | 1980 | 7000967 |
cook/chill foodservice system with a microwave oven: aerobic plate counts from beef loaf, potatoes and frozen green beans. | the purpose was to evaluate microbiological quality and end temperature (et) of portioned food after heating in a microwave oven as used in a hospital cook/chill foodservice system. beef loaf (15 kg), potatoes (6 kg), and green beans (5 kg) were prepared in a laboratory. after initial cooking to 60 degrees c, and storage (7 degrees c for 24 h), beef loaf (100 g) was microwave heated: 20, 50, 80 or 110 s. potatoes were reconstituted, stored (7 degrees c for 24 h), portioned (100 g/portion), and m ... | 1980 | 7003155 |
immunocytochemical localization of ingested kidney bean (phaseolus vulgaris) lectins in rat gut. | the binding of ingested kidney bean (phaseolus vulgaris) lectins to the luminal surface of the rat gut was investigated by an indirect immunofluorescence method in which the primary antiserum was monospecific for kidney bean globulin lectins. the major lectin-positive sites were found to be the non-crypt regions of villi in the proximal region of the small intestine; exactly the same regions displayed extensive disruption of microvilli. lectins were not localized in the brush border regions of i ... | 1980 | 7005194 |
kidney bean (phaseolus vulgaris) lectin-induced lesions in rat small intestine: 2. microbiological studies. | | 1980 | 7024332 |
purification and properties of chloroplast leucyl-trna synthetase from a higher plant: phaseolus vulgaris. | the chloroplast leucyl-trna synthetase from phaseolus vulgaris was purified by ammonium sulfate precipitation and chromatography on deae-cellulose, hydroxylapatite, and phosphocellulose. finally, the pure enzyme was obtained after affinity chromatography on blue sepharose cl-6b using specific elution with a pure escherichia coli trnaleu isoacceptor. the specific activity of chloroplast leucyl-trna synthetase (1550 units/mg) is the highest ever obtained for a higher plant aminoacyl-trna synthetas ... | 1982 | 7050099 |
a lectin-resistant mouse lymphoma cell line is deficient in glucosidase ii, a glycoprotein-processing enzyme. | glycosylation of asparagine residues of glycoproteins occurs by the transfer of a glucose3mannose9n-acetylglucosamine2 (glc3man9glcnac2) oligosaccharide from a lipid carrier to the nascent protein. normally, this transfer is quickly followed by the stepwise removal of the glucose residues which are arranged in the sequence: glc1 leads to 2glc1 leads to 3glc1 leads to 3man. we now report studies which demonstrate that a lectin-resistant mutant of the bw5147 mouse lymphoma cell line is deficient i ... | 1982 | 7050108 |
inhibitors of animal cell-free protein synthesis from grains. | we described earlier the purification and properties of a protein (tritin) from wheat that enzymatically inhibits translation in cell-free systems from animals but not plants. in this report, we have examined 11 additional grains (family gramineae) and three other seeds for the presence of tritin-like proteins. in addition to wheat species, barley, oats, rye, triticale and corn were found to be sources of inhibitor; no inhibitor could be detected in rice, millet, sesame, alfalfa, mung bean or co ... | 1982 | 7066324 |
recent advances in the study of the nutritional toxicity of kidney bean (phaseolus vulgaris) lectins in rats. | the main toxic component isolated from several varieties of kidney bean (phaseolus vulgaris) is a haemagglutinating lectin. the inclusion in rat diets of raw kidney beans or purified bean lectins results in abnormal development of microvilli in the small intestine. immunocytochemical investigations have provided evidence that this lesion is associated with the binding of lectins to the luminal surfaces of enterocytes. the lectin-induced disruption of intestinal microvilli may result in interfere ... | 1982 | 7080034 |
studies on phaseolus vulgaris phytohemagglutinin. structural requirements for simple sugars to inhibit the agglutination of human group a erythrocytes. | | 1982 | 7106298 |
bacteriocin production by pseudomonas syringae psw-1 in plant tissue. | the production and activity of syringacin w-1, a particulate bacteriocin made by pseudomonas syringae psw-1, was studied in plant tissue. the bacteriocin is rod shaped, approximately 20 nm wide and 75 nm long, and composed of an outer sheath and inner core. both the producing strain, psw-1, and a sensitive strain, 16, grew within red kidney bean stems. strains psw-1 and 16, or mutants derived from them, were injected into bean stems singly or in mixtures. all singly inoculated strains grew well. ... | 1982 | 7116232 |
characterization of the structural determinants required for the high affinity interaction of asparagine-linked oligosaccharides with immobilized phaseolus vulgaris leukoagglutinating and erythroagglutinating lectins. | the carbohydrate binding specificities of the leukoagglutinating phytohemagglutinin (l-pha) and erythroagglutinating phytohemagglutinin (e-pha) lectins of the red kidney bean, phaseolus vulgaris, have been investigated by lectin-agarose affinity chromatography of asn-linked oligosaccharides. high affinity binding to e-pha-agarose occurs only with biantennary glycopeptides containing 2 outer galactose residues and a residue of n-acetylglucosamine linked beta 1,4 to the beta-linked mannose residue ... | 1982 | 7118880 |
use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa. | paraffin-embedded human bronchial biopsies, obtained in macroscopically healthy areas, were examined using nine peroxidase-bound lectins. these were either isolated or purified by affinity column chromatography (ulex europeus, triticum vulgare, glycine max, and arachis hypogatea lectins) or commercial preparations (lotus tetragonolobus, canavalia ensiformis, helix pomatia, phaseolus vulgaris, and lens culinaris lectins) and conjugated to peroxidase (except for concanavalin a). these labeled lect ... | 1982 | 7130674 |
the lethal protein from kintoki beans (phaseolus vulgaris) identified as a lectin. | a glycoprotein isolated from kintoki beans (phaseolus vulgaris cultivar kintoki) agglutinated human erythrocytes of all types and erythrocytes of rat, rabbit, sheep, and mouse. the lectin activity was not affected by 1 hr heating at 60 degrees c, but decreased slightly on heating for the same period at 70-80 degrees c and markedly at 90-100 degrees c. the activity was inhibited by galactose, lactose, n-acetyl galactosamine and fetuin. the inhibition was, however, weak, as often found for nonspec ... | 1982 | 7131101 |
kidney bean (phaseolus vulgaris) lectin-induced lesions in rat small intestine. 3. ultrastructural studies. | | 1982 | 7142451 |
[disorders appearing in animals ingesting vicia faba l. beans]. | the effect of feeding growing male rats and chickens on diets containing raw field bean (vicia faba l.) as the main source of protein, on the rate of growth and muscle protein metabolism, has been investigated for more than ten years in this laboratory. in the present report we summarize some of the results we have obtained so far. the experimental animals showed, along with the classical reduction in the rate of growth, significant increases in several urinary parameters. these effects were acc ... | 1982 | 7146591 |
circular dichroism studies on conformational transitions of phytohemagglutinins effected by some alcohols. | the effects of methanol, ethanol, 1-propanol and 2,2,2,-trifluoroethanol (tfe) on conformation of 10 phytohemagglutinins (lectins) were examined by applying the circular dichroism (cd) method. concanavalin a and the lectins isolated from pea (pisum sativum), lentil (lens culinaris), soybean (glycine max), peanut (arachis hypogaea), dolichos biflorus, sophora japonica, lotus tetragonolobus, ulex europaeus i, and phaseolus vulgaris (leucoagglutinin) were used. slight changes in the structure of th ... | 1982 | 7150429 |
the effect of heating on the haemagglutinating activity and nutritional properties of bean (phaseolus vulgaris) seeds. | | 1982 | 7166934 |
artocarpus integrifolia lectin(s): use and applications in chromosome studies of lymphocyte cultures. | a simple procedure for extracting a lectin from the seeds of the jackfruit, artocarpus integrifolia, is described. the extracts had an average protein concentration of 13.3 mg/ml, and were strongly erythroagglutinating with a 10(6) titre. erythroagglutination was shown to be non-specific for a1-b-0 erythrocytes. lymphoblastic transformation and mitogenic stimulation occurred in lymphocyte cultures both in man and rhesus monkey, and chromosome preparations were obtained. such preparations were of ... | 1982 | 7172755 |
protein utilization and toxic effects of raw beans (phaseolus vulgaris) for young pigs. | one balance trial and two pair-feeding experiments were conducted to determine the effects of feeding raw small red beans (phaseolus vulgaris) on dietary protein utilization, organ weights, pancreatic enzyme activities, blood variables and weight gain of young pigs. in the balance trial, the substitution of 5 and 15% raw beans for soybean meal and cornstarch in the semipurified diet depressed apparent digestibilities of crude protein, total s and all individual amino acids measured. urinary excr ... | 1982 | 7174552 |
differences of glycoconjugates exposed on hypernephroma and normal kidney cells. | we analyzed the outer cell membranes of normal kidney cells, hypernephroma cells, nude mouse transplants of hypernephroma tissue, and cell lines derived from hypernephroma using horseradish peroxidase-coupled lectins of different specificity, in order to investigate their binding to cryostat sections or the cell surfaces of suspended single cells. we showed that hypernephroma cells express higher amounts of receptors for robinia pseudoacacia lectin, soy bean lectin and phaseolus vulgaris lectin ... | 1982 | 7176041 |
[significance of various antiphysiological and nutritional factors on the biological evaluation of various cultures of the common bean (phaseolus sp.)]. | raw and cooked samples of beans supplemented with methionine were used to prepare 35 rat diets containing different amounts of methionine, tannins, and trypsin inhibitors. the effect of these diets on weight gain, mortality and protein utilization was measured. the weight gain/protein consumption ratio increased while mortality decreased as the proportion of raw beans decreased in the diet. the effects of diet, beans and their interaction were significant. supplementation with methionine had no ... | 1982 | 7186348 |
effect of raw legume diets on disaccharidase activity in the small intestine of chicks. | the effect of four raw legume diets: field beans (vicia faba) (rfb), navy beans (phaseolus vulgaris) (rnb), soybeans (glycine soja) (rsb) and bitter vetch (vicia ervilia) (rbv), on disaccharidase activities in chick small intestine have been studied. maltase and sucrase activities, which vary with age, were determined in 1 to 60 day old animals, rfb and rbv diets had no effect on maltase activity and only increased sucrase activity in 60 day old chicks. both maltase and sucrase activities decrea ... | 1980 | 7192009 |
on characterization of phaseolus vulgaris lectin (pha) & separation of homogeneous leucoagglutinin (l-pha). | | 1980 | 7203549 |
structure of a complex-type sugar chain of human glycophorin a. | tryptic glycopeptide t1 of human glycophorin a [tomita, m., & marchesi, v. t. (1975) proc. natl. acad. sci. u.s.a. 72, 2964-2968] was subjected to hydrazinolysis. after n-acetylation, the complex-type oligosaccharides were isolated by gel filtration. the major neutral oligosaccharide (2000 molecular weight) was purified by a combination of ion-exchange and gel-permeation chromatography. treatments with endo- and exo-glycosidases, periodate oxidation, and methylation analysis indicated that the m ... | 1981 | 7213594 |
immunochemical detection of ds-rna in healthy and virus-infected plants and specific detection of viral ds-rna by hybridization to labelled complementary dna. | double-stranded (ds) rna was detected in nucleic acid preparations from virus-free leaf tissues of several plant species by radial double-diffusion tests with antiserum raised against polyinosinic : polycytidylic acid [poly(i) : poly(c)]. no significant differences in the concentrations of ds-rna were detected by such tests in virus-free french bean leaves and those infected with tobacco mosaic virus or tobacco ringspot virus. however, virus-specific ds-rnas as well as genomic rnas of both virus ... | 1981 | 7228978 |
differences in the biochemical compositions and elicitor activity of extracellular components produced by three races of a fungal plant pathogen, colletotrichum lindemuthianum. | high molecular weight products from the alpha, beta, and gamma races of colletotrichum lin- differed in their carbohydrate and protein compositions and in their abilities to elicit symptoms of a hypersensitive response in dark red kidney bean. the neutral sugar composition of the products varied in their proportions of rhamnose, mannose, galactose, and glucose. polyacrylamide gel electrophoresis showed the protein components to be more numerous in the beta race products than in the alpha or gamm ... | 1980 | 7237271 |
guanidinoacetate methyltransferase activity in growing male rats fed on a raw field bean (vicia faba l.) diet. | a significant reduction (p less than 0.01) in both the rate of growth and liver protein was found in growing male rats fed ad libitum over a 5-week period on a diet containing raw field bean (vicia faba l.) as the sole source of protein, as compared to casein-fed rats. the activity of the hepatic enzyme guanidinoacetate methyltransferase (ec 2.1.1.2) as well as the urinary output of total nitrogen and creatinine were also significantly increased (p less than 0.01) in the legume-fed rats. the add ... | 1981 | 7238472 |
comparison of phaseolus vulgaris cultivars on the basis of isolectin differences. | | 1981 | 7238987 |
protein quality in seed meals of phaseolus vulgaris and heat-stable factors affecting the utilisation of protein. | | 1981 | 7253585 |
anabolic action of dianabol (17 alpha-methyl-17beta-hydroxyandrosta-1, 4-dien-3-one) on the nitrogen balance and skeletal muscle composition of rats fed on field bean (vicia faba l.). | the addition of dianabol (17alpha-methyl-17beta-hydroxyandrosta-1,4-dien-3-one) (1 mg/100 g of diet) to a diet containing raw field bean (vicia faba l.) as the sole source of protein has been studied in young male rats, fed for two weeks on the legume diet, as compared to casein-fed animals. a significant improvement (p less than 0.02) in the biological value and net protein utilization was found in the rats fed the legume diet with dianabol added. furthermore, a significant reduction (p less th ... | 1981 | 7274996 |
kidney bean (phaseolus vulgaris) lectin-induced lesions in the small intestine: 1. light microscope studies. | | 1980 | 7276267 |
protein digestibility and protein quality of common beans (phaseolus vulgaris) fed alone and with maize, in adult humans using a short-term nitrogen balance assay. | various experiments using young human adults were carried out to measure the protein digestibility of common beans, fed alone and in different forms and fed together with maize as tortillas. in all, 36 young men were used. common bean protein digestibility was significantly lower than that observed from meat, and similar to the digestibility of a textured vegetable protein soy protein product. it was also observed that forms of consumption may affect protein digestibility of beans. using a short ... | 1981 | 7282614 |
separation and localization on sodium dodecyl sulfate polyacrylamide gels of pig spleen lymphocyte plasma membrane proteins which bind 125i-labelled phytohemagglutinin. | the protein constituents of splenic pig lymphocytes derived from the plasma membranes, the endoplasmic reticulum or from a non-ionic (nonidet p-40) detergent of whole cells have been separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, under reducing and non-reducing conditions. the binding of phaseolus vulgaris (red kidney bean) phytohemagglutinin to these constituents has been studied by incubation of the labelled lectin with the undried electrophoretograms. results show cha ... | 1981 | 7284434 |
the toxicity of phaseolus vulgaris lectins. nitrogen balance and immunochemical studies. | | 1981 | 7300257 |
intestinal absorption of d-galactose and l-leucine and intestinal disaccharidase activities in growing chickens fed different raw legume diets. | a significant (p less than .01) impairment in the rate of growth, along with a significant (p less than .01) inhibition in the rate of in vivo intestinal absorption of d-galactose and l-leucine, and in the in vitro intestinal absorption of d-galactose, was found in growing chickens fed ad libitum over a 60-day period, diets containing the raw legumes vicia faba, glycine soja, vicia ervilia, and phaseolus vulgaris as the main source of protein. furthermore, a significant (p less than .01) reducti ... | 1981 | 7301750 |
phytomitogen-induced stimulation of syntheses de novo of phosphatidylinositol, phosphatidic acid and diacylglycerol in rat and human lymphocytes. | the biosynthetic route of phosphatidylinositol in lymphocytes stimulated with either concanavalin a or phaseolus vulgaris hemagglutinin is examined by the determination of the [2-3h]glycerol and 32po4 incorporations into glycerolipids of rat and human lymphocytes. in rat lymph-node cells, the [2-3h]glycerol incorporation into phosphatidylinositol was accelerated 23-fold by 50 microgram concanavalin a/ml culture; the same cells exhibited a 37-fold concanavalin a-induced stimulation in 32po4 incor ... | 1981 | 7306564 |
comparison of yeast and mold counts by spiral, pour, and streak plate methods. | foods with naturally occurring yeast and mold were used in a comparative study of 4 plating techniques. oats, green beans, and cheese were incubated for 3 and 5 days at 25 degrees c. although 3 days of incubation would be sufficient for yeast, 5 days were necessary for the mold count. the percent recovery of yeast and molds by the spiral and streak methods ranged from 200 to 357% compared with the pour plating method described in the bacteriological analytical manual (bam). the spiral method had ... | 1981 | 7309670 |
intestinal absorption of d-glucose, d-galactose and l-leucine in male growing rats fed a raw field bean (vicia faba l.) diet. | male growing rats were fed diets containing either raw field bean (vicia faba l.) or casein as the only source of protein. diets were fed ad libitum for 5 weeks. body weight gain was significantly impaired, and rates of both in vivo and in vitro intestinal absorption of d-glucose, d-galactose and l-leucine were reduced, in rats fed the legume diet. the addition of methionine to the bean diet did not alter either growth or intestinal absorption rates. no significant differences in intestinal oxyg ... | 1981 | 7319945 |
[possible relationship of physical, chemical and nutritional measurements of the common bean (phaseolus vulgaris)]. | | 1981 | 7344644 |
[digestion of field bean cytoplasmic carbohydrates by the preruminant calf]. | | 1981 | 7349548 |
an inhibitor of lymphocyte proliferation produced by a human colonic adenocarcinoma cell line in culture. | the culture fluid taken from a human colonic adenocarcinoma cell line, skco-1, inhibited mitogenic stimulation of mouse lymphocytes measured by reductions in blast information or [3h]thymidine uptake. the inhibitor was not cytotoxic to lymphocytes nor did it alter the growth rates of 21 other cell lines examined. however, the in vitro growth rates of two murine lymphoid tumor lines were also markedly reduced by the skco-1 medium. the tumor cell culture media could be added up to 40 hr after conc ... | 1980 | 7349901 |
effect of raw legume diets on intestinal absorption of d-galactose by chick. | the effect of four raw legume diets on the intestinal absorption of d-galactose and oxygen consumption were studied in chick. field beans (vicia faba), soybeans (glycine soja), bitter vetch (vicia ervilia), and navy beans (phaseolus vulgaris), were used. the intestinal absorption was determined by both in vivo and in vitro techniques. in vivo, only navy beans and soybeans inhibit intestinal transport of d-galactose, while in vitro all the diets do. oxygen consumption by intestinal rings increase ... | 1980 | 7394275 |
a new human erythrocyte variant (ph) containing an abnormal membrane sialoglycoprotein. | 1. a new human erythrocyte variant (ph) is described. the variant contains an unusual sialic acid-rich glycoprotein in addition to the blood-group-mn([unk])- and blood-group-ss(delta)-active sialoglycoproteins found in normal erythrocytes. 2. the unusual component ph has an apparent mol.wt. of 32000 on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. the ph component is not degraded during trypsin treatment of intact erythrocytes. 3. the ph component was labelled by lacto-peroxidase-m ... | 1980 | 7396858 |
binding of chemical derivatives of phaseolus vulgaris phytohemagglutinin to pig spleen lymphocytes. | the mitogenic activity of phaseolus vulgaris phytohemagglutinin (pha) and its acetyl, citraconyl, glycinyl, homoarginyl, n-bromosuccinimide, and nitro derivatives has been assayed using pig spleen lymphocytes. nitrated and n-bromosuccinimide-treated pha were not mitogenic. whereas the mitogenicity of acetyl pha was impaired, the other derivatives were as active as untreated lectin. binding studies of [125i]pha and derivatives to purified pig spleen lymphocytes were performed at 37 degrees c. res ... | 1980 | 7407677 |
elicitor modulation of the turnover of l-phenylalanine ammonia-lyase in french bean cell suspension cultures. | (1) the mechanisms underlying the transient increase in phenylalanine ammonia-lyase activity during phaseollin accumulation in cell suspension cultures of dwarf french bean (phaseolus volgaris) have been investigated using density labelling with 3h from 2h2o coupled with residual analysis of the equilibrium distribution of enzyme activity in high-resolution kbr density gradients. (2) the resolution achieved in this system is sufficient to allow quantitative analysis of the relative proportions o ... | 1980 | 7459387 |
effect of heat treatment on the toxicity and nutritive value of dry bean (phaseolus vulgaris var. rosinha g2) proteins. | | 1980 | 7462520 |
lectin receptors on human blood and bone marrow cells and their use in cell separation. | a series of fluorescein-conjugated lectins (sophora japonica agglutinin, helix pomatia agglutinin, peanut agglutinin, the erythroagglutinin from phaseolus vulgaris, pokeweed mitogen, wheat germ agglutinin and the fucose-binding lectin from lotus tetragonolobus) were analyzed for their binding to human peripheral blood cells with a fluorescence-activated cell sorter. most of the lectins showed increasing cell binding in the order erythrocytes less than lymphocytes less than monocytes less than ne ... | 1980 | 7470630 |
morphological evidence for the sprouting of inhibitory commissural fibers in response to the lesion of the excitatory entorhinal input to the rat dentate gyrus. | recently a commissural fiber projection that terminates in the outer molecular layer of the fascia dentata was described in normal rats (deller et al., 1995). in the present article, phaseolus vulgaris leucoagglutinin (phal) tracing was used to analyze the contribution of this previously unknown projection to the commissural sprouting response after entorhinal cortex lesion. rats 4-8 weeks after unilateral entorhinal lesion received a single phal deposit into the hilus of the fascia dentata cont ... | 1995 | 7472444 |
cholinergic basal forebrain neurons project to cortical microvessels in the rat: electron microscopic study with anterogradely transported phaseolus vulgaris leucoagglutinin and choline acetyltransferase immunocytochemistry. | physiological evidence indicates that central cholinergic pathways are involved in the regulation of cerebral cortical blood flow. we investigated the possible contribution of basal forebrain cholinergic neurons from the substantia innominata (si) to the innervation of cortical microvessels. basalo-cortical perivascular nerve terminals were detected by light and electron microscopic immunocytochemistry of anterogradely transported phaseolus vulgaris leucoagglutinin (pha-l) following its injectio ... | 1995 | 7472495 |
the superior colliculus relays signals descending from the vibrissal motor cortex to the facial nerve nucleus in the rat. | electrical stimulation of the vibrissal motor cortex (vmcx), the superior colliculus and the facial nerve nucleus elicits vibrissal movements. a possibility of the superior colliculus as one of the relay nuclei between the vmcx and the facial nerve nucleus was investigated by injecting an anterograde neural tracer, phaseolus vulgaris leucoagglutinin (pha-l), into the physiologically identified vmcx and a retrograde tracer, cholera toxin b subunit (ctb), into the facial nerve nucleus in wistar ra ... | 1995 | 7478258 |
anatomical evidence for inputs to ventrolateral medullary catecholaminergic neurons from the midbrain periaqueductal gray of the rat. | previous studies have shown that the midbrain periaqueductal gray (pag) projects to the ventrolateral medulla (vlm). here, we studied pag projections to the area of a1/c1 neurons in the vlm in the rat using phaseolus vulgaris leucoagglutinin (pha-l) anterograde tracing combined with immunocytochemistry for tyrosine hydroxylase (th) or phenylethanolamine n-methyl transferase (pnmt). following pag injections, pha-l labeled fibers and terminals were intermingled among th-immunoreactive (th-ir) neur ... | 1995 | 7478270 |
stress activation of a bean hydroxyproline-rich glycoprotein promoter is superimposed on a pattern of tissue-specific developmental expression. | the hrgp4.1 gene, which encodes a cell wall hydroxyproline-rich glycoprotein, was isolated from a genomic library of bean (phaseolus vulgaris l.). two transcripts, one induced by wounding and one by elicitation, were transcribed from the same initiation site. the gene encodes a polypeptide of 580 amino acids with the amino terminal half consisting of repeats of the sequence serine-(proline)4-lysine-histidine-serine-(proline)4-(tyrosine)3-histidi ne and the carboxyl-terminal half composed of repe ... | 1995 | 7480331 |
plasmodium gallinaceum: sporozoite invasion of aedes aegypti salivary glands is inhibited by anti-gland antibodies and by lectins. | there is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. we are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. we used an in vivo bioassay for sporozoite invasion of salivary glands. in this assay, purified sporozoites from mature oocytes of plasmodium gallinaceum were injected into aedes aegypti mosquitoes and salivary glands were dissected at different time points after inj ... | 1995 | 7498430 |
efferent projections of the paraventricular thalamic nucleus in the rat. | the paraventricular nucleus of the thalamus (pvt) receives input from all major components of the circadian timing system, including the suprachiasmatic nucleus (scn), the intergeniculate leaflet and the retina. for a better understanding of the role of this nucleus in circadian timing, we examined the distribution of its efferent projections using the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l). the efferent projections of the pvt are loosely organized along its dorsal-ventral ... | 1995 | 7499526 |
intratelencephalic projections of the visual wulst in pigeons (columba livia). | the visual wulst is the telencephalic target of the thalamofugal visual pathway of birds, and thus the avian equivalent of the striate cortex of mammals. the anterograde tracer phaseolus vulgaris leucoagglutinin was used to follow the intratelencephalic connections of the major constituents of the visual wulst in pigeons. in particular, efferent pathways from the granular layer (intercalated nucleus of the hyperstriatum accessorium, iha), supragranular layer (hyperstriatum accessorium, ha), and ... | 1995 | 7499547 |
fastigiofugal projection to the brainstem nuclei in the cat: an anterograde pha-l tracing study. | fastigial projections to brainstem nuclei were studied using an anterograde neural tracer, phaseolus vulgaris leucoagglutinin (pha-l). microinjections of pha-l were made into the rostral pole, and middle and caudal parts of the left fastigial nucleus in cat. in addition to fastigioreticular and fastigiovestibular projections, fastigiofugal projections to cranial motor nuclei (iv, vi and vii) and those nuclei involved in autonomic control were identified. at the medullary level, a topographic arr ... | 1995 | 7501304 |
dip: a member of the mip family of membrane proteins that is expressed in mature seeds and dark-grown seedlings of antirrhinum majus. | dip, a gene from antirrhinum majus, which encodes a protein with striking homology to other integral membrane proteins, was cloned. the gene was specifically expressed in mature seeds and during seedling germination, particularly in cotyledons of seedlings grown in the dark. the deduced product, called dip, for dark intrinsic protein, shows strong homology with the mip family of channel transporters which include; the bovine major intrinsic protein (mip), the escherichia coli glycerol facilitato ... | 1993 | 7504555 |
pancreatic enlargement is evident in rats fed diets containing raw soybeans (glycine max) or cowpeas (vigna unguiculata) for 800 days but not in those fed diets based on kidney beans (phaseolus vulgaris) or lupinseed (lupinus angustifolius). | pancreatic weights and composition were studied with rats fed diets containing raw legume seeds for up to 800 d. rapid pancreatic enlargement was induced by dietary soybeans (glycine max) (high kunitz and bowman-birk trypsin inhibitor contents, moderate lectin content) during the initial 150 d. over the next 200 d the rate of pancreatic growth was similar to that in controls. after 350 d a second period of rapid pancreatic growth occurred. macroscopic pancreatic nodules were evident in a number ... | 1993 | 7505319 |
a phaseolus lectin anterograde tracing study of the rotundo-telencephalic projections in the domestic chick. | phaseolus vulgaris leucoagglutinin extracellular injection was performed in nucleus rotundus thalami and the course of labelled fibers and their termination were examined in chicken. the labelled fibers terminate in ectostriatum centrale, ectostriatum perifericum and neostriatum intermedium. the course and termination of the fibers are analysed in detailed. | 1993 | 7505789 |
projections from the nucleus tractus solitarii to the spinal cord. | projections from the nucleus tractus solitarii (nts) to the spinal cord were demonstrated in the male sprague-dawley rat. in retrograde transport studies, a horseradish peroxidase conjugate or a fluorescent dye, fluorogold, were injected into midcervical or upper thoracic spinal segments. most solitariospinal neurons were multipolar or bipolar and located between the obex and spinomedullary junction. solitariospinal neurons were concentrated in proximity to the ventral border of the solitary tra ... | 1993 | 7506272 |
complementary immunolocalization patterns of cell wall hydroxyproline-rich glycoproteins studied with the use of antibodies directed against different carbohydrate epitopes. | antisera raised against the major hydroxyproline-rich glycoprotein (hrgp) in carrot (daucus carota l.) taproot, extensin-1, and a minor hrgp, extensin-2, were characterized by western blot analysis, enzyme-linked immunosorbent assay, and periodate oxidation and found to be directed against carbohydrate epitopes shared by both glycoproteins. the anti-extensin-1 antibodies (ge1) target periodate-sensitive epitopes and may recognize the terminal alpha-1,3-arabinoside of extensin-1. the anti-extensi ... | 1993 | 7506427 |
direct projections from the anterior thalamic nuclei to the retrohippocampal region in the rat. | the present study examined the areal and laminar distribution of direct projections from the anterior thalamic nuclei to the retrohippocampal region in the rat, with anterograde transport of phaseolus vulgaris-leucoagglutinin. the anteromedial nucleus (am) projects to the temporal subiculum, medial entorhinal area, perirhinal area, and caudomedial part of the lateral entorhinal area. the interanteromedial nucleus (iam) projects to the perirhinal area and the caudolateral part of the lateral ento ... | 1993 | 7506716 |
connectivity of fetal neocortical block transplants in the excitotoxically ablated cortex of adult rats. | fetal neocortical block grafts placed into newborn recipients are able to exchange axonal projections with the host central nervous system, as shown in several previous experiments. the present study examined the connectivity of fetal neocortical block transplants placed into the excitotoxically ablated cortex of adult rats. young adult rats received injections of the excitotoxic amino acid n-methyl-d-aspartate into the sensorimotor cortex area 1 week prior to receiving a fetal (e14-15) neocorti ... | 1993 | 7507863 |
use of phaseolus vulgaris leukoagglutinating lectin in histochemical and blotting techniques: a comparison of digoxigenin- and biotin-labelled lectins. | an increase in the number of beta 1,6 branches of the trimannosyl core of asparagine-linked oligosaccharides has been shown to be directly correlated with the metastatic potential of cultured tumour cells. the phaseolus vulgaris leukoagglutinating lectin (pha-l) binds to beta 1,6 branches of tri- and tetra-antennary oligosaccharides. we have applied digoxigenin- and biotin-conjugated pha-l to establish a non-radioactive detection system for beta 1,6 branches, which can be used in lectin blotting ... | 1993 | 7508428 |
computer-assisted reconstruction of axonal arborizations anterogradely labelled with the phaseolus vulgaris leucoagglutinin technique. | this paper describes a computer-assisted method which allows the tracing of axonal arborizations, and the reconstruction in a either grey-scale or a binary image representation, of all labelled neuronal processes located within different focal planes of a single tissue section. the system includes a nikon microscope connected to a ccd video camera, and a macintosh microcomputer. each step of the method is described in order to show that the capturing of images, retouching and pasting are easily ... | 1993 | 7509017 |
organization of rodent auditory cortex: anterograde transport of pha-l from mgv to temporal neocortex. | in the present study we analyzed the organization of the thalamocortical projections of the specific auditory relay nucleus of the thalamus, the ventral division of the medial geniculate body (mgv), using the anterograde axonal tracer phaseolus vulgaris leucoagglutinin. all injections of mgv produced dense labeling of axonal fibers in temporal cortex. in all cases, labeled axons were predominantly concentrated in cortical layers iii and iv and, to a lesser extent, at the junction of layers v and ... | 1993 | 7511011 |
information cascade from primary auditory cortex to the amygdala: corticocortical and corticoamygdaloid projections of temporal cortex in the rat. | corticocortical and corticoamygdaloid connections of temporal cortext and perirhinal cortex (prh) were examined in the rat with the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l). iontophoretic injections of pha-l into area te1 resulted in columnar axonal terminations in surrounding and contralateral regions of temporal neocortex and in the striatum, but not in the amygdala. within temporal neocortex, labeled fibers were present locally in adjacent regions of te1, as well as in te ... | 1993 | 7511012 |
lipopolysaccharide epitope expression of rhizobium bacteroids as revealed by in situ immunolabelling of pea root nodule sections. | to investigate the in situ expression of lipopolysaccharide (lps) epitopes on nodule bacteria of rhizobium leguminosarum, monoclonal antibodies recognizing lps macromolecules were used for immunocytochemical staining of pea nodule tissue. many lps epitopes were constitutively expressed, and the corresponding antibodies reacted in nodule sections with bacteria at all stages of tissue infection and cell invasion. some antibodies, however, recognized epitopes that were only expressed in particular ... | 1994 | 7511581 |
the peripheral and central projections of the edinger-westphal nucleus in the rat. a light and electron microscopic tracing study. | the peripheral and central efferent projections of the rostral part of the edinger-westphal nucleus in the rat were investigated at the light and electron microscopic level by means of iontophoretic injections of the anterograde tracer phaseolus vulgaris-leucoagglutinin and retrograde tracer injections of fast blue and nuclear yellow into the facial nucleus and into the principal olive. two pathways leaving the rostral part of the edinger-westphal nucleus were studied, a peripheral and a central ... | 1993 | 7511978 |
topographic distribution of efferent fibers originating from homotopic or heterotopic transplants: heterotopically transplanted neurons retain some of the developmental characteristics corresponding to their site of origin. | the present study was undertaken to determine whether the topographical distribution of cortical efferents is exclusively dependent on environmental cues or is also controlled by intrinsic factors. for the purpose, we used a sensitive tract-tracing method (phaseolus vulgaris leucoagglutinin) to compare the pattern of efferent fibers of homotopic and heterotopic transplants of embryonic (e16) neocortex. our findings indicate that transplants of embryonic sensorimotor cortex placed homotopically i ... | 1994 | 7513624 |
multiple anterograde tracing, combining phaseolus vulgaris leucoagglutinin with rhodamine- and biotin-conjugated dextran amine. | the simultaneous use of different neuroanatomical anterograde tracers provides a potentially powerful method to study the convergence of afferent systems in a particular brain area. however, a simple routine procedure to apply multiple anterograde tracers in conjunction with their simultaneous visualization is still missing. we report an easy and straightforward application of three sensitive anterograde tracers: phaseolus vulgaris leucoagglutinin (pha-l), rhodamine-conjugated dextran amine (rda ... | 1994 | 7514701 |
organization of the efferent projections from the pontine parabrachial area to the bed nucleus of the stria terminalis and neighboring regions: a pha-l study in the rat. | the organization of efferent projections from the pontine parabrachial (ppb) area to the forebrain rostral to the central nucleus of the amygdala (ce) was studied in the rat by using microinjections of phaseolus vulgaris leucoagglutinin (pha-l), into subregions of the ppb area. the present study is a follow-up of a former study (bernard et al. [1993] j. comp. neurol. 329:201-229) which examines ppb projections onto the ce. the results demonstrate that: (1) the ppb(m) region (the medial, the vent ... | 1994 | 7515078 |
direct projections from the ventrolateral medulla oblongata to the limbic forebrain: anterograde and retrograde tract-tracing studies in the rat. | neurons in the ventrolateral medulla oblongata, a brain region implicated in central vasomotor regulation, have previously been reported to project to some forebrain limbic structures. the aim of the present study was (1) to describe the termination pattern of ventral medullary afferents in forebrain limbic areas using anterograde tract tracing, and (2) to determine the location and some morphological characteristics of the projection neurons using retrograde tract tracing from selected forebrai ... | 1994 | 7516349 |
reciprocal connections of lateral septal neurons and neurons in the lateral hypothalamus in the rat: a combined phaseolus vulgaris-leucoagglutinin and fluoro-gold immunocytochemical study. | reciprocal connections between lateral septal neurons and neurons in the lateral hypothalamus/lateral preoptic area were studied in the rat. the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) and the retrograde tracer fluoro-gold (fg) were simultaneously injected into the lateral septum. after double-immunocytochemistry, pha-l-labeled terminals were found in synaptic contact with dendrites of retrogradely fg-labeled neurons in the lateral hypothalamic/lateral preoptic area. | 1994 | 7518057 |
cytochemical staining for beta 1,6 branching of asparagine-linked oligosaccharides in variants of metastatic human colon carcinoma cells. | a positive correlation between tumor progression in human colon and increased beta 1,6 branching in oligosaccharides has recently been demonstrated. the present study was undertaken to elucidate whether such a correlation can be extended to variants of metastasizing human colon carcinoma hct116 cells. the phaseolus vulgaris leukoagglutinating lectin, which binds to beta 1,6 branched oligosaccharides, was employed. in blots, a band of approximately 140 kd was detectable in both the hct116a and hc ... | 1994 | 7519829 |
cholecystokinin corticostriatal pathway in the rat: evidence for bilateral origin from medial prefrontal cortical areas. | the anterograde tracer phaseolus vulgaris-leucoagglutinin was used to examine the organization of the projections to the striatum from medial prefrontal and frontal cortical areas in the rat with reference to their relation to cholecystokinin-like immunoreactivity in the striatum. medial prefrontal cortical areas projected bilaterally, with an ipsilateral predominance, to the striatum. most of the positive fibres were found in medial and ventral areas of the caudate-putamen and in the nucleus ac ... | 1994 | 7520138 |
gabaergic projection from the intercalated cell masses of the amygdala to the basal forebrain in cats. | the intercalated cell masses (icms) are dense clusters of small gabaergic cells interposed between the basolateral and centromedial nuclear groups of the amygdala. until now, the icms have been largely ignored in anatomical studies of the amygdaloid complex. thus, this study was undertaken to identify some of their targets by means of tract-tracing methods combined with immunohistochemical techniques. wheat-germ agglutinin conjugated to horseradish peroxidase (wga-hrp) was injected into numerous ... | 1994 | 7520456 |
input-output organization of the rat vibrissal motor cortex. | the afferent and efferent connections of the vibrissal area of the rat motor cortex (vmcx) were investigated by injecting phaseolus vulgaris leucoagglutinin (pha-l) or wheat germ agglutinin-horseradish peroxidase into the physiologically defined vmcx. the vmcx formed reciprocal connections with the primary and secondary somatosensory cortex, lateral and ventrolateral orbital cortex, retrosplenial cortex, and perirhinal cortex. these corticocortical afferents originated from cell bodies in layers ... | 1994 | 7523173 |
commissural connections of the cat periaqueductal gray matter studied with anterograde and retrograde tract-tracing techniques. | the commissural connections of the periaqueductal gray matter were investigated by light and electron microscopy by using the anterograde tracer phaseolus vulgaris leucoagglutinin and the retrograde tracer horseradish peroxidase. in the first group of seven animals (1-7), single injections of phaseolus vulgaris leucoagglutinin were performed iontophoretically (4.5 microa for 30 min) into various subdivisions of the periaqueductal gray matter. on light microscopic examination, injection sites wer ... | 1994 | 7523986 |
organization of vestibular inputs to nucleus tractus solitarius and adjacent structures in cat brain stem. | the vestibular system is involved in maintaining stable blood pressure and respiration during changes in posture and is essential for eliciting motion sickness-related vomiting. because the nucleus tractus solitarius (nts) participates in the regulation of sympathetic and inspiratory outflow and the triggering of emesis, we tested the hypothesis that this region receives vestibular inputs in cats. in one set of experiments, microinjections of the tracer phaseolus vulgaris leucoagglutinin into th ... | 1994 | 7524372 |
projections of the suprachiasmatic nuclei, subparaventricular zone and retrochiasmatic area in the golden hamster. | the patterns of projections from the hamster suprachiasmatic nucleus, retrochiasmatic area and subpraventricular hypothalamic zone were examined using anterograde tracing with the plant lectin, phaseolus vulgaris leucoagglutinin. suprachiasmatic nucleus efferents comprise four major fiber groups: (i) an anterior projection to the ventral lateral septum, the bed nucleus of the stria terminalis and anterior paraventricular thalmus; (ii) a periventricular hypothalamic projection extending from the ... | 1994 | 7526267 |
projections from the primary auditory cortex onto the dorsal cortex of the inferior colliculus in albino rats. | the topography of connections from the primary auditory cortex (te1) onto the dorsal cortex (dc) of the inferior colliculus (ic) was studied using the anterograde neural tracers phaseolus vulgaris (pha-l) and biotinylated dextran amine (bda). injections in different restricted portions of the ventral te1 showed labelled axonal sheets in the ipsilateral dc with extensions in the central nucleus and the external cortex. also contralateral labelled axons were found with a patchy appearance. the pat ... | 1994 | 7526815 |
em study on terminals labelled by phaseolus vulgaris lectin in ectostriatum periphericum and neostriatum intermedium. | the terminals of rotundal fibers labelled with phaseolus lectin anterograde tracer were studied in ectostriatum periphericum and neostriatum intermedium laterale with em. these terminals belong to the fibers which cross the ectostriatum centrale before they enter the ectostriatum periphericum and neostriatum intermedium laterale. these terminals establish synapses with dendrites, spines and also with gaba immunogold stained somata. the em results prove the connections of ectostriatum periphericu ... | 1994 | 7527065 |
preoptic projections to barrington's nucleus and the pericoerulear region: architecture and terminal organization. | the medial preoptic area (mpo), a sexually dimorphic region, plays a pivotal role in neuroendocrine function and reproductive behavior. we recently reported that mpo projects heavily to the midbrain periaqueductal gray (pag). we also noted that mpo projects to the dorsolateral pontine tegmentum. here we identified the cells of origin of the mpo-->tegmental projection and delineated the terminal organization of mpo projections to barrington's nucleus, locus coeruleus (lc), and the rostromedial pe ... | 1994 | 7528227 |
efferent connections of the medial prefrontal cortex in the rabbit. | the different cytoarchitectonic regions of the medial prefrontal cortex (mpfc) have recently been shown to play divergent roles in associative learning in rabbits. to determine if these subareas of the mpfc, including areas 24 (anterior cingulate cortex), 25 (infralimbic cortex), and 32 (prelimbic cortex) have differential efferent connections with other cortical and subcortical areas in the rabbit, anterograde and retrograde tracing experiments were performed using the phaseolus vulgaris leukoa ... | 1994 | 7529194 |
a direct pretectosuprachiasmatic projection in the rat. | the major afferent projections of the suprachiasmatic nuclei originate in the retina and the intergeniculate leaflet of the lateral geniculate nucleus and are important in the entrainment of endogenous circadian rhythms. a characteristic feature of the suprachiasmatic nucleus and the intergeniculate leaflet of the thalamus is that they are bilaterally innervated from the retina. however, parts of the olivary and posterior pretectal nuclei have been shown to be bilaterally innervated from the ret ... | 1994 | 7530345 |
principal cells are the postsynaptic targets of supramammillary afferents in the hippocampus of the rat. | neurons of the supramammillary nucleus are known to fire phase-locked to hippocampal theta rhythm. stimulation of this area induces theta activity in the hippocampus via the medial septum and facilitates perforant pathway stimulation-evoked population spikes in the dentate gyrus even if the medial septum is inactivated. this latter effect was suggested to be due to a direct inhibitory input from the supramammilary nucleus to hippocampal nonpyramidal cells resulting in disinhibition. in the prese ... | 1994 | 7531093 |
termination pattern of medial hyperstriatum ventrale efferents in the archistriatum of the domestic chick. | the chick archistriatum receives afferents from the intermediate part of the medial hyperstriatum ventrale (imhv) and projects to the lobus parolfactorius (lpo). there is functional evidence to suggest that the imhv and the lpo are connected, but there is no anatomical evidence for a direct connection between the two structures. the aim of the current study was to characterize the termination pattern of medial hyperstriatal afferents within the archistriatum to determine whether the archistriatu ... | 1994 | 7531206 |
modifying actions of solvent extracts from fruit and vegetable residues on 2-amino-3-methylimidazo[4,5-f]quinoline (iq) and 2-amino-3,4-dimethylimidazo[4,5-f]quinoxaline (meiqx) induced mutagenesis in salmonella typhimurium ta 98. | the edible parts of 13 fruits--apples, apricots, bananas, blackberries, sweet cherries, red currants, white grapes, oranges, peaches, pears, plums, raspberries, and strawberries--and of 12 vegetables--asparagus, green beans, broccoli, brussels sprouts, red and white cabbage, carrots, cauliflower, onions, green peppers, spinach, and tomatoes--were squeezed in order to separate juices and residues. the residues were washed, lyophilized, and extracted sequentially with n-hexane, dichloromethane, ac ... | 1995 | 7531289 |