| structure and nucleotide sequence of euphorbia characias copper/tpq-containing amine oxidase gene. | a cdna encoding for a copper containing amine oxidase has been isolated and sequenced from young leaves of euphorbia characias, a perennial mediterranean shrub. a single long open reading frame of 2068 pb encodes a protein composed of 653 amino acids with a molecular mass of about 74 kda. a putative 24-aminoacid signal peptide precedes the sequence of the mature protein, with characteristics of a secretion signal peptide. alignments of euphorbia amine oxidase cdna nucleotide sequence with that o ... | 2002 | 12523646 |
| inositol phosphate stimulation by lh requires the entire alpha asn56 oligosaccharide. | lentil lectin-bound, fucose-enriched htsh was reported to stimulate both camp and inositol phosphate (ip) intracellular signalling pathways, whereas fucose-depleted htsh stimulated only the camp pathway. gonadotropins activate the camp pathway and in several studies higher concentrations activate the ip pathway. since only the 10% of alpha subunit asn(56) oligosaccharides (asn(52) in humans) are fucosylated, the higher glycoprotein hormone concentrations required for ip pathway activation might ... | 2003 | 12581881 |
| genetics of resistance to anthracnose and identification of aflp and rapd markers linked to the resistance gene in pi 320937 germplasm of lentil (lens culinaris medikus). | anthracnose, caused by colletotrichum truncatum, is a major disease problem and production constraint of lentil in north america. the research was conducted to examine the resistance to anthracnose in pi 320937 lentil and to identify molecular markers linked to the resistance gene in a recombinant inbred line (ril) population developed from a cross of eston lentil, the susceptible parent, and pi 320937, the resistant parent. a total of 147 f(5:6) rils were evaluated for resistance to anthracnose ... | 2003 | 12589542 |
| crystal structure of pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose. | the crystal structure of the man/glc-specific seed lectin from pterocarpus angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. the carbohydrate binding site contains a classic man/glc type specificity loop. its metal binding loop on the other hand is of the long type, different from what is observed in other man/glc-specific legume lectins. glucose binding in the primary binding site is reminiscent of the glucose complexes of concanavalin a and lentil lectin. ... | 2003 | 12595543 |
| purification and properties of hydrophilic dimers of acetylcholinesterase from mouse erythrocytes. | differences in the glycosylation of acetylcholinesterase (ache) subunits which form the dimers of mouse erythrocyte and a suitable procedure to purify the enzyme by affinity chromatography in edrophonium-sepharose are described. ache was extracted ( approximately 80%) from erythrocytes with triton x-100 and sedimentation analyses showed the existence of amphiphilic ache dimers in the extract. the ache dimers were converted into monomers by reducing the disulfide bond which links the enzyme subun ... | 2003 | 12672481 |
| the binding characteristics and utilization of aleuria aurantia, lens culinaris and few other lectins in the elucidation of fucosyltransferase activities resembling cloned ft vi and apparently unique to colon cancer cells. | human colon carcinoma cell fucosyltransferase (ft) in contrast to the fts of several human cancer cell lines, utilized glcnacbeta1,4glcnacbeta-o-bn as an acceptor, the product being resistant to alpha1,6-l-fucosidase and its formation being completely inhibited by lacnac type 2 acceptors. further, this enzyme was twofold active towards the asialo agalacto glycopeptide as compared to the parent asialoglycopeptide. only 60% of the glcnac moieties were released from [14c]fucosylated asialo agalacto ... | 2003 | 12681913 |
| is the catalytic mechanism of bacteria, plant, and mammal copper-tpq amine oxidases identical? | this short review is mostly concerned with the work carried out in rome on the copper amine oxidase from bovine serum (bsao). the first target was the copper oxidation state and its relationship with the organic cofactor. it was found that copper is not reduced on reaction with amines under anaerobic conditions or along the catalytic cycle and that it is not within bonding distance of the quinone cofactor. the copper stability in the oxidised state was supported by bsao ability to oxidise benzyl ... | 2003 | 12686125 |
| adhesion of salmonella enterica serotype enteritidis isolates to chicken isthmal glandular secretions. | the ability of salmonella enterica serotype enteritidis isolates to adhere to immobilized secretions of the isthmus of the laying hen was determined in an elisa-type assay. one-third of the 56 isolates tested in the logarithmic growth phase, adhered to the isthmal secretions. using a binding assay of the isolates to thin paraffin sections of the oviduct, we demonstrated that the receptor of the adhesion was localized inside the tubular gland cells of the isthmus. the adhesion to immobilized isth ... | 2003 | 12695046 |
| digestion rate of legume carbohydrates and glycemic index of legume-based meals. | a study was performed to examine the rate of digestion of available carbohydrate in legumes and its mixtures with cereals, prepared as commonly eaten. the legumes and cereals studied were lentil (lens sculenta), pea (pisum sativum), bean (phaseolus vulgaris, var tortola), rice (oryza sativa) and spaghetti. foods were purchased at the city market. total starch content and the carbohydrate digestion rates were determined using the enzymatic method proposed by englyst et al. total starch levels ran ... | 2003 | 12701368 |
| selective binding of lectins to embryonic chicken vasculature. | chicken embryos are an excellent model system for studies related to vascular morphogenesis. development in ovo allows manipulations otherwise difficult in mammals, and the use of chicken-quail chimeras offers an additional advantage to this experimental system. furthermore, the chicken chorioallantoic membrane has been extensively used for in vivo assays of angiogenesis. surprisingly, few markers are available for a comprehensive visualization of the vasculature. here we report the use of lecti ... | 2003 | 12704207 |
| effect of heat treatment and mineral and vitamin supplementation on the nutritive use of protein and calcium from lentils (lens culinaris m.) in growing rats. | the effects of heat treatment, supplementation of a mineral and vitamin premix, and 4% olive oil on the bioavailability of protein and calcium from lens culinaris m., var vulgaris, cultivar. magda-20 were studied in growing rats. | 2003 | 12714100 |
| [legume cross-reactivity]. | legumes are dicotyledonous plants belonging to the fabales order. the main distinctive characteristic of which is their fruit (legumen, seeds contained in pods). this botanical order is formed by three families: mimosaceae, caesalpiniaceae and papilionaceae or fabacea. the papilionaceae family includes the most important allergenic species: lens culinaris (lentil), cicer arietinum (chick-pea), pisum sátivum (pea), arachis hipogea (peanut), phaseolus vulgaris (bean) y glycine max (soy). legumes a ... | 2003 | 12783766 |
| characterization of the 8-kilodalton antigens of taenia solium metacestodes and evaluation of their use in an enzyme-linked immunosorbent assay for serodiagnosis. | the western blot for cysticercosis, which uses lentil lectin purified glycoprotein (llgp) antigens extracted from the metacestode of taenia solium, has been the "gold standard" serodiagnostic assay since it was first described in 1989. we report that the diagnostic antigens at 14, 18, and 21 kda, as well as some larger disulfide-bonded antigens, are actually all members of a very closely related family of proteins, the 8-kda antigens. the genes for 18 unique, mature proteins have been identified ... | 2003 | 12791883 |
| economy of fertilizer nitrogen through organic sources in rain-fed rice-legume cropping systems in west bengal, india. | field experiments were conducted at a farmers" plot adjacent to the regional research station, red and laterite zone, sub-center sekhampur (birbhum district) of west bengal, india, situated 23 degrees 24' n latitude, 87 degrees 24' e longitude, to study the effect of different bio- and organic sources of nutrients instead of total fertilizer n in terms of crop productivity in the sequence and building up of soil fertility. during the wet seasons of 1997 and 1998, 12 combinations of bio- and orga ... | 2001 | 12805756 |
| lectin typing of five medically important candida species. | this study reports the patterns of agglutination of 93 clinical candida isolates by 14 commercial lectins. the isolates were of the species candida albicans (55), c. tropicalis (12), c. guilliermondii (10). c. glabrata (eight) and c. parapsilosis (eight). hundred percent of isolates were agglutinated, at least, by a panel of three lectins: canavalia ensiformis (cona), lens culinaris (lca) and pisum sativum (psa), all of them with alpha-d-mannose specificity. in addition, another panel of three l ... | 2003 | 12870195 |
| soil properties and crop productivity as influenced by flyash incorporation in soil. | field experiments were carried out during 1996-97 at gulawathi, muthiani and salarpur villages, iari farm, new delhi and ncpp campus, dadri to evaluate changes in soil characteristics and growth of wheat (triticum aestivum l.), mustard (brassica juncea l.), lentil (lence esculenta moench.), rice (oryza sativa l.) and maize (zea mays l.) by varying amounts of flyash addition (up to 50t ha(-1)) in soils at sowing/transplanting time of crops. flyash addition in areas adjoining ncpp thermal power pl ... | 2003 | 12889638 |
| determination of folate concentrations in pulses by a microbiological method employing trienzyme extraction. | over the past two decades, the role of folate in human nutrition has been of much interest because of its relationship to diseases such as neural tube defects and heart disease. since 1998, the u.s. food and drug administration has mandated that cereal products be fortified with 140 microg of folic acid/100 g. it is important, therefore, to be able to determine accurately the folate concentrations in cereals and other grains to ensure proper dietary intake of folate. in this study, a microbiolog ... | 2003 | 12926876 |
| recent advances in basic and applied science for the control of taeniasis/cysticercosis in asia. | detection of seven specific bands by immunoblot (ib) using glycoproteins (gps) purified by lentil-lectin affinity chromatography has been the gold-standard for neurocysticercosis (ncc) serodiagnosis since 1989. however, due to the presence of contaminants, it was impossible to apply the gps to elisa. our group at asahikawa medical college (amc) succeeded in purifying the gps by preparative isoelectric focusing; these higher quality gps were suitable for elisa. based on the results of both ib and ... | 2002 | 12971481 |
| the leafy vegetable amaranth (amaranthus gangeticus) is a potent inhibitor of calcium availability and retention in rice-based diets. | improvement in the nutritional quality of bangladeshi rice-based diets is sought through increasing the amounts of vegetables, fish and legumes consumed. the aim of the present study was to compare the effects of rice-based diets on selected parameters of ca and p availability and retention in young, growing rats. the study was designed as a randomised balance trial with five diets, eight animals per diet, and two balance periods of 1 week each. apart from diet 1, which was a pure rice diet, the ... | 2003 | 13129457 |
| a case of mimicry in plants-vicia sativa l. in lentil crops. | | 1959 | 14439712 |
| novel core(polyester)-shell(polysaccharide) nanoparticles: protein loading and surface modification with lectins. | this study describes new lectin-decorated or protein-loaded nanoparticles with a hydrophobic poly(epsilon-caprolactone) (pcl) core and a hydrophilic dextran (dex) corona. in this view, a family of block dex-pcln copolymers was first synthesized, consisting of a dex backbone to which n preformed pcl blocks were grafted. the ability of these new copolymers to form nanoparticles was evaluated in comparison with a series of pcl homopolymers of various molecular weights (2000, 10,000 and 40,000 g/mol ... | 2003 | 14499189 |
| partial characterisation of carbohydrate-rich echinococcus granulosus coproantigens. | coproantigen elisa based tests for diagnosis of canine echinococcosis provide high specificity and sensitivity. however, the antigenic molecules present in faeces from infected dogs have not yet been characterised. while initial attempts to determine the molecular weights of echinococcus granulosus coproantigens by sds-page and western blotting with coproantigen reactive capture antibodies were equivocal, they suggested presence of a significant carbohydrate component. periodate treatment of cop ... | 2003 | 14572518 |
| occupational asthma in an agronomist caused by the lentil pest bruchus lentis. | | 2003 | 14616139 |
| golgi dynamics during meiosis are distinct from mitosis and are coupled to endoplasmic reticulum dynamics until fertilization. | one current theory of the golgi apparatus views its organization as containing both a matrix fraction of structural proteins and a reservoir of cycling enzymes. during mitosis, the putative matrix protein gm130 is phosphorylated and relocalized to spindle poles. when the secretory pathway is inhibited during interphase, gm130 redistributes to regions adjacent to vesicle export sites on the endoplasmic reticulum (er). strikingly, meiotic maturation and fertilization in nonrodent mammalian eggs pr ... | 2003 | 14623231 |
| characterization and cloning of gp50, a taenia solium antigen diagnostic for cysticercosis. | gp50, a taenia solium protein diagnostic for cysticercosis has been cloned, sequenced, and characterized. gp50 is one diagnostic component of the lentil lectin purified glycoprotein (llgp) antigens that have been used for antibody-based diagnosis of cysticercosis in a western blot assay for nearly 15 years. gp50 is a glycosylated and gpi-anchored membrane protein. the native protein migrates at 50kda, but the predicted molecular weight of the mature protein is 28.9. antigenically active recombin ... | 2004 | 14668018 |
| identification, purification and partial characterization of tissue inhibitor of matrix metalloproteinase-2 in bovine pulmonary artery smooth muscle. | bovine pulmonary artery smooth muscle possesses the tissue inhibitor of matrix metalloproteinase-2 (timp-2) as revealed by western immunoblot study of its cytosol fraction with bovine polyclonal timp-2 antibody. this potent polypeptide inhibitor of matrix metalloproteinases (mmps) was purified to homogeneity from cytosol fraction of bovine pulmonary artery smooth muscle. this inhibitor was purified by ammonium sulfate precipitation followed by gelatin sepharose and lentil lectin sepharose affini ... | 2003 | 14674707 |
| reaction of lentil trypsin-chymotrypsin inhibitors with human and bovine proteinases. | four trypsin-chymotrypsin inhibitors from syrian local small lentils were selected to study the reasons for their different action against human and bovine proteinases. chemical modification experiments, enzymatic modifications followed by carboxypeptidase degradation, and characterization of the inhibitor/enzyme complexes formed were performed. all four lens culinaris inhibitors (lci) contained arginine at the trypsin-reactive site, and tyrosine (lci-1.7 and lci-2.2), phenylalanine (lci-3.3), o ... | 2003 | 14690394 |
| differential effect of lentil feeding on proteosynthesis rates in the large intestine, liver and muscle of rats. | the aim of this work was to test the hypothesis that the trophic effect of lentil feeding on large intestine results from a stimulation of protein synthesis and to determine whether it interferes with protein metabolism in other splanchnic or peripheral organs. two groups of growing sprague dawley male rats were pair-fed iso-caloric iso-nitrogenous balanced diets containing either cooked lentils (lens esculenta puyensis) or casein as unique protein source. protein synthesis rates were measured i ... | 2004 | 14711455 |
| the chickpea, summer cropping, and a new model for pulse domestication in the ancient near east. | the widely accepted models describing the emergence of domesticated grain crops from their wild type ancestors are mostly based upon selection (conscious or unconscious) of major features related either to seed dispersal (nonbrittle ear, indehiscent pod) or free germination (nondormant seeds, soft seed coat). based on the breeding systems (self-pollination) and dominance relations between the allelomorphs of seed dispersal mode and seed dormancy, it was postulated that establishment of the domes ... | 2003 | 14737827 |
| [bioprotection mechanisms of the lentil plant by rhizobium leguminosarum against fusarium oxysporum f. sp. lentis]. | living and heat-killed bacterial cells of rhizobium leguminosarum protected totally lentil plants against infection by the pathogen fusarium oxysporum mr 84. culture filtrate of this rhizobacterium was also able to protect the plants to a high degree. however, when they were inoculated separately of the pathogen, living bacterial cells did not protect the plants whereas culture filtrate and killed bacterial cells protected them. these results suggest that rhizobium cannot protect lentil plants w ... | 2003 | 14746271 |
| reconstitution of atp-dependent cgmp transport into proteoliposomes by membrane proteins from human erythrocytes. | the cellular efflux of cgmp from human erythrocytes has previously been characterized in functional studies. the purpose of the present study was to find membrane proteins with the ability to restore atp-dependent uptake of cgmp into proteoliposomes. human erythrocyte membranes were solubilized with chaps (3-([3-cholamidopropyl]dimethylammonio)-1-propanesulfonate) and gel filtration gave three protein fractions with the ability to restore active transport. only two of these fractions were retain ... | 2004 | 15025427 |
| carbohydrate structure and differential binding of prostate specific antigen to maackia amurensis lectin between prostate cancer and benign prostate hypertrophy. | serum prostate-specific antigen (psa) assay is widely used for detection of prostate cancer. because psa is also synthesized from normal prostate, false positive diagnosis cannot be avoided by the conventional serum psa test. to apply the cancer-associated carbohydrate alteration to the improvement of psa assay, we first elucidated the structures of psa purified from human seminal fluid. the predominant core structure of n-glycans of seminal fluid psa was a complex type biantennary oligosacchari ... | 2004 | 15044396 |
| membrane cofactor protein is a receptor for adenoviruses associated with epidemic keratoconjunctivitis. | subgroup d adenovirus (ad) types 8, 19, and 37 (ad8, -19, and -37, respectively) are causative agents of epidemic keratoconjunctivitis and genital tract infections. previous studies showed that ad37 binds to a 50-kda membrane glycoprotein expressed on human ocular (conjunctival) cells. to identify and characterize the role of the 50-kda glycoprotein in ad37 infection, we partially purified this molecule from solubilized chang c conjunctival cell membranes by using lentil lectin chromatography an ... | 2004 | 15047806 |
| tissue distribution, inhibition and activation of gelatinolytic activities in atlantic cod (gadus morhua). | gelatinolytic activities in fish tissues with properties like matrix metalloproteinases (mmps) have been paid little attention. however, they have been proposed to participate in post mortem degradation during storage and the disintegration of pericellular connective tissue during spawning. in this paper the distribution of gelatinolytic activities in liver, heart, muscle, gill, and male and female gonad of atlantic cod (gadus morhua) was studied by using gelatin sds-page, proteinase inhibitors, ... | 2004 | 15050523 |
| role of capsule in serotypic differences and complement fixation by lactococcus garvieae. | seventeen geographically distinct isolates of lactococcus garvieae, isolated from diseased fish, were compared serologically using antiserum raised against the various isolates in rainbow trout. sera raised against a capsule deficient isolate did not agglutinate capsulated isolates, regardless of origin. in contrast, all antisera raised against capsulated isolates cross reacted strongly with non-capsulated isolates. antisera raised against capsulated japanese isolates cross reacted with other ca ... | 2004 | 15123324 |
| differential fermentation of glucose-based carbohydrates in vitro by human faecal bacteria--a study of pyrodextrinised starches from different sources. | pyrodextrins, modified starches produced by heat/acid treatment, have been used extensively in the paper industry. recently, pyrodextrinisation has been recognised as a way of producing a "resistant starch" that is water-soluble and has non-starch linkages. however, a full characterisation of the fermentation properties of pyrodextrins has not been reported. | 2004 | 15168041 |
| taenia solium metacestode glycoproteins as diagnostic antigens for solitary cysticercus granuloma in indian patients. | taenia solium metacestode glycoproteins specific for lentil lectin were evaluated as diagnostic antigens for solitary cysticercus granulomas in indian patients, using both an elisa and immunoblotting. in 250 patients suspected to have neurocysticercosis and subjected to a computerized tomography scan or magnetic resonance imaging, the proteins were diagnostic by the elisa in 86 patients (80%) and by immunoblots in 67 (62%) of 107 patients with solitary cysticerus granuloma. among 100 non-cystice ... | 2004 | 15186936 |
| solid substrate production of epicoccum nigrum conidia for biological control of brown rot on stone fruits. | production of conidia of epicoccum nigrum, a biocontrol agent of the fungal pathogen monilinia laxa, was tested in liquid- and solid-state fermentation. liquid fermentation was conducted in 250 ml erlenmeyer flasks containing 50 ml of a mineral medium (containing per litre: 20 g lactose, 10 g no3k, 1 g k2hpo4, 0.5 g mgso4.7h2o, and 1 ml of a minor-element solution), inoculated with 2 x 10(5) e. nigrum conidia ml(-1), and incubated at 20-25 degrees c and 150 rpm for 7 days. solid-state fermentati ... | 2004 | 15193802 |
| susceptibility of bovine germinal vesicle-stage oocytes from antral follicles to direct effects of heat stress in vitro. | delineation of maternal versus direct effects of heat stress in reducing development at the germinal vesicle (gv) stage is challenging, because oocytes spontaneously resume meiosis after removal from antral follicles. the use of s-roscovitine (inhibitor of p34(cdc2)/cyclin b kinase) to hold bovine oocytes at the gv stage without compromising early embryo development was previously validated in our laboratory. the objective of the present study was to assess the direct effects of an elevated temp ... | 2004 | 15201201 |
| complete amino acid sequence of the lentil trypsin-chymotrypsin inhibitor lci-1.7 and a discussion of atypical binding sites of bowman-birk inhibitors. | the complete primary structure of the lentil (lens culinaris) trypsin-chymotrypsin inhibitor lci-1.7 was determined by conventional methods in order to find relationships between partial sequences and the difference in action against human and bovine chymotrypsin. as other bowman-birk type inhibitors, lci-1.7 contained 68 amino acid residues, seven disulfide bridges, and two reactive sites, arg16-ser17 for trypsin and tyr42-ser43 for chymotrypsin. evaluation of sequence homologies showed that it ... | 2004 | 15212472 |
| distribution of prepubertal and adult goat oocyte cortical granules during meiotic maturation and fertilisation: ultrastructural and cytochemical study. | the aim of this study was evaluate cortical granule (cg) distribution during in vitro maturation (ivm) and fertilisation of prepubertal goat oocytes compared to cg distribution of ovulated and in vitro fertilised oocytes from adult goats. oocytes from prepubertal goats were recovered from a slaughterhouse and were matured in m199 with hormones and serum for 27 hr. ovulated oocytes were collected from gonadotrophin treated murciana goats. frozen-thawed spermatozoa were selected by centrifugation ... | 2004 | 15236337 |
| resistance of glycine species and various cultivated legumes to the soybean aphid (homoptera: aphididae). | the soybean aphid, aphis glycines matsumura, is a new pest of soybean, glycine max (l.) merr., in north america. it has become widespread on soybean in north america since it was first identified in the midwest in 2000. species of rhamnus l. (buckthorn) are the primary hosts of a. glycines, and soybean is known as a secondary host. there is limited information about the secondary host range of a. glycines. aphid colonization on various legume hosts was compared in choice experiments. aphid colon ... | 2004 | 15279293 |
| potentiation of insecticidal activity of bacillus thuringiensis subsp. kurstaki hd-1 by proteinase inhibitors in the american bollworm, helicoverpa armigera (hübner). | the effect of crude proteinase inhibitor extracts from seeds of different crop plants (black gram, chickpea, chickling vetch, finger millet, french bean, green gram, horse gram, lentil, pea and soybean) on the insecticidal activity of b. thuringiensis var. kurstaki hd-1 was investigated against neonate larvae of h. armigera by diet incorporation method. the larval mortality due to crude proteinase inhibitors alone (5% seed weight equivalent) ranged from 4.1 to 19.1%; the maximum mortality with f ... | 2004 | 15282948 |
| isolation of a family of resistance gene analogue sequences of the nucleotide binding site (nbs) type from lens species. | most known plant disease-resistance genes (r genes) include in their encoded products domains such as a nucleotide-binding site (nbs) or leucine-rich repeats (lrrs). sequences with unknown function, but encoding these conserved domains, have been defined as resistance gene analogues (rgas). the conserved motifs within plant nbs domains make it possible to use degenerate primers and pcr to isolate rgas. we used degenerate primers deduced from conserved motifs in the nbs domain of nbs-lrr resistan ... | 2004 | 15284869 |
| bioavailability of phytic acid-phosphorus and magnesium from lentils (lens culinaris m.) in growing rats: influence of thermal treatment and vitamin-mineral supplementation. | we sought to improve the nutritive utilization of lens culinaris m. variety vulgaris cultivar magda-20 in growing rats by autoclaving the lentil seeds at 120 degrees c for 30 min at an internal pressure of 1 atm and by supplementing the rats with a vitamin-mineral premix. | 2004 | 15325690 |
| differential effects of cooked common bean (phaseolus vulgaris) and lentil (lens esculenta puyensis) feeding on protein and nucleic acid contents in intestines, liver and muscles in rats. | our aim was to investigate the influence of legume feeding on the protein and nucleic acid content of intestinal tissues and muscles. | 2004 | 15334033 |
| affinity chromatography with monolithic capillary columns i. polymethacrylate monoliths with immobilized mannan for the separation of mannose-binding proteins by capillary electrochromatography and nano-scale liquid chromatography. | monolithic capillary columns with surface-immobilized mannan have been introduced for affinity-based micro-column separations by nano-liquid chromatography (nano-lc) and capillary electrochromatography (cec). two kinds of polymethacrylate monoliths were prepared, namely poly(glycidyl methacrylate-co-ethylene dimethacrylate) and poly(glycidyl methacrylate-co-ethylene dimethacrylate-co-[2-(methacryloyloxy)ethyl]trimethyl ammonium chloride) to yield neutral and cationic macroporous polymer, respect ... | 2004 | 15354437 |
| porcine antibody responses to taenia solium antigens rgp50 and sts18var1. | cysticercosis, a disease caused by the larval form of taenia solium, is diagnosed by detection of specific antibodies or by imaging techniques. our preferred immunologic assay for cysticercosis is the enzyme-linked immunoelectrodifusion transfer blot, or immunoblot, using the lentil lectin bound antigens from larval cysts. antibody reactivity with any one of seven glycoproteins is diagnostic for cysticercosis. to develop a simple antibody detection assay for field use, we have synthesized an 8-k ... | 2004 | 15381814 |
| newer markers for hepatocellular carcinoma. | the incidence of hepatocellular carcinoma (hcc) is increasing worldwide; the overall survival of patients with hcc is grim because most patients are diagnosed late, when curative treatment is not possible. cirrhosis is the strongest risk factor for the development of hcc. hcc surveillance with alpha-fetoprotein (afp) and ultrasonography has been recommended for persons with cirrhosis. however, afp level is insensitive for the early detection of hcc, and ultrasonography is expensive and operator ... | 2004 | 15508074 |
| maintenance of meiotic arrest in bovine oocytes using the s-enantiomer of roscovitine: effects on maturation, fertilization and subsequent embryo development in vitro. | the overall objective was to evaluate the effectiveness of the s-enantiomer of roscovitine (inhibitor of p34cdc2/cyclin b kinase) to maintain bovine cumulus-oocyte complexes at the germinal vesicle (gv) stage for extended times after removal from antral follicles without compromising subsequent maturation, fertilization and embryo development. oocytes were cultured in 0, 12.5, 25 or 50 micromol/l s-roscovitine for 24 h. hoechst staining showed that 50 micromol/l s-roscovitine maintained >90% of ... | 2005 | 15615895 |
| [effect of luteinized granulosa cell conditioned medium on cortical granule of mouse oocytes matured in vitro]. | to study the effect of luteinized granulosa cell conditioned medium on cortical granule (cg) of the mouse oocytes matured in vitro. | 2004 | 15634500 |
| magnesium-content of rhizobium nodules in different plants: the importance of magnesium in nitrogen-fixation of nodules. | rhizobium bacteria induce nodules (tumors) in roots of leguminous crops that fix nitrogen (n2) from the atmosphere. | 2004 | 15637228 |
| a genetic linkage map of lens sp. based on microsatellite and aflp markers and the localization of fusarium vascular wilt resistance. | microsatellites have currently become the markers of choice for molecular mapping and marker-assisted selection for key traits such as disease resistance in many crop species. we report here on the mapping of microsatellites which had been identified from a genomic library of lentil (lens culinaris medik.). the majority of microsatellite-bearing clones contained imperfect di-nucleotide repeats. a total of 41 microsatellite and 45 amplified fragment length polymorphism (aflp) markers were mapped ... | 2005 | 15650814 |
| moulds and yeasts in fresh and minimally processed vegetables, and sprouts. | a limited survey of fresh and minimally processed vegetables, and sprouts was conducted in the washington, dc area to determine if potentially toxigenic and pathogenic fungi were present in these commodities. thirty-nine ready-to-eat salads, 29 whole fresh vegetables and 116 sprout samples (bean, alfalfa, broccoli, crunchy, garlic, spicy, onion, clover, lentil and multi-seed sprouts) were purchased from 13 local supermarkets and tested for yeast and mould counts as well as the presence of toxige ... | 2005 | 15718030 |
| serological markers of liver cancer. | serological markers for hepatocellular carcinoma (hcc) are important for early diagnosis, as well as monitoring of tumour aggressiveness, treatment responsiveness, recurrence and survival. the three most common markers are total alpha-fetoprotein (afp), lens culinaris agglutinin-reactive afp (afp-l3) and protein induced by vitamin k absence or antagonist-ii (pivka-ii). total afp has the sensitivity of 60% and specificity of 90% for the detection of hcc. increase in the percentage of afp-l3 over ... | 2005 | 15757806 |
| nutritional quality of microwave-cooked and pressure-cooked legumes. | eight whole legumes, namely bengal gram (cicer arietinum), broad beans (vicia faba), cowpea (vigna catjang), field beans (dolichos lablab), green gram (phaseolus aureus roxb), horse gram (dolichos biflorus), lentils (lens esculenta) and french beans (phaseolus vulgaris), were cooked under pressure or in a microwave oven and were analysed for nutrient composition. raw legumes served as control. the range of nutrients analysed in 100 g cooked samples were as follows: moisture, 62.8-69.7 g; protein ... | 2004 | 15762308 |
| application of synthetic 8-kd and recombinant gp50 antigens in the diagnosis of neurocysticercosis by enzyme-linked immunosorbent assay. | the gold standard serodiagnostic assay for cysticercosis and neurocysticercosis, diseases caused by the metacestode of taenia solium, uses lentil lectin-purified glycoprotein (llgp) in a western blot assay. we tested two antigens derived from llgp, synthetic ts18var1 (sts18var1) and recombinant gp50 antigen (rgp50), in an enzyme-linked immunosorbent assay (elisa) using serum and cerebrospinal fluid (csf) samples. the sensitivity for serum and csf was 94.7% and 100% for rgp50 and 90.4% and 90.2% ... | 2005 | 15772321 |
| usefulness of the novel oncofetal antigen glypican-3 for diagnosis of hepatocellular carcinoma and melanoma. | glypican-3 (gpc3) mrna and protein are expressed in >80% of human hepatocellular carcinomas (hcc) but not in normal tissues except for placenta and fetal liver. the oncofetal antigen gpc3 is a glycosylphosphatidyl inositol-anchored membrane protein and may be secreted. it is a novel tumor marker for human hcc: gpc3 protein was present in sera from 40-50% of hcc patients, but was not detected in sera from patients with liver cirrhosis or chronic hepatitis, or in sera from healthy individuals. alp ... | 2005 | 15807627 |
| bifidogenic effect of dietary fiber and resistant starch from leguminous on the intestinal microbiota of rats. | the bifidogenic effect of leguminous containing diets on the intestinal microbiota of male wistar rats was studied. | 2005 | 15850967 |
| increased sialylation and defucosylation of plasma proteins are early events in the acute phase response. | within hours of turpentine injection to stimulate the acute phase (ap) response in rats, the n-acetylneuraminic acid content of plasma proteins increases and that of fucose decreases, each by about 60%. the two changes are inversely related (r = -0.97). the neuac/gal ratio increases from the normal 0.75 to 1.0 on day 2 of the ap. whereas 50% of the isolated oligosaccharides of normal plasma proteins are retarded on immobilized ricinus communis agglutinin, those from day 2 ap plasma fail to do so ... | 2005 | 15858075 |
| [evaluation of the iron bioavailability from meals prepared with lentil (lens esculenta) or with white kidney bean seeds (phaseolus vulgaris) in anemic rats]. | the aim of our study was to evaluate the iron bioavailability from lentil seeds and white kidney bean seeds. the iron bioavailability from diets was measured in rat by the depletion-repletion hemoglobin method and the reserve of iron stored in the liver. our results showed that the iron bioavailability from meal based on cooked lentil seeds was significantly low (3.9 +/- 0.9%) as compared to the reference group fed with ferrous sulfate (20.4 +/- 4.9%) (p < 0.01) or to the group fed with cooked w ... | 2005 | 15966663 |
| reactions of plant copper/topaquinone amine oxidases with n6-aminoalkyl derivatives of adenine. | plant copper/topaquinone-containing amine oxidases (caos, ec 1.4.3.6) are enzymes oxidising various amines. here we report a study on the reactions of caos from grass pea (lathyrus sativus), lentil (lens esculenta) and euphorbia characias, a mediterranean shrub, with n6-aminoalkyl adenines representing combined analogues of cytokinins and polyamines. the following compounds were synthesised: n6-(3-aminopropyl)adenine, n6-(4-aminobutyl)adenine, n6-(4-amino-trans-but-2-enyl) adenine, n6-(4-amino-c ... | 2005 | 15968819 |
| mechanism-based inactivators of plant copper/quinone containing amine oxidases. | copper/quinone amine oxidases contain cu(ii) and the quinone of 2,4,5-trihydroxyphenylalanine (topaquinone; tpq) as cofactors. tpq is derived by post-translational modification of a conserved tyrosine residue in the protein chain. major advances have been made during the last decade toward understanding the structure/function relationships of the active site in cu/tpq amine oxidases using specific inhibitors. mechanism-based inactivators are substrate analogues that bind to the active site of an ... | 2005 | 16054177 |
| comparative study of the conformational lock, dissociative thermal inactivation and stability of euphorbia latex and lentil seedling amine oxidases. | the thermal stability of copper/quinone containing amine oxidases from euphorbia characias latex (elao) and lentil seedlings (lsao) was measured in 100 mm potassium phosphate buffer (ph 7.0) following changes in absorbance at 292 nm. elao was shown to be about 10 degrees c more stable than lsao. the dissociative thermal inactivation of elao was studied using putrescine as substrate at different temperatures in the range 47-70 degrees c, and a "conformational lock" was developed using the theory ... | 2005 | 16096724 |
| simple and rapid method for the differentiation of lens culinaris medik. from false lentil species. | the practice of using both common vetch (vicia sativa l.) and single-flower vetch (vicia articulata hornem.) seeds as food or feed is encouraged by the very high resemblance of their seeds with those of small-seeded lentil cultivars. among the vicieae, antinutritional and toxic factors are particularly important, because many species, containing high levels of these compounds, are not safe. a simple and fast capillary electrophoresis (ce) method was proposed for the differentiation of lentil cul ... | 2005 | 16104771 |
| purification and functional reconstitution of the rat organic cation transporter oct1. | the rat organic cation transporter roct1 with six histidine residues added to the c-terminus was expressed in sf9 insect cells, and expression of organic cation transport was demonstrated. to purify roct1 protein, sf9 cells were lysed with 1% (w/v) chaps [3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate], centrifuged, and subjected to sequential affinity chromatography using lentil-lectin sepharose and nickel(ii)-charged nitrilotriacetic acid-agarose. this procedure yielded approximatel ... | 2005 | 16142924 |
| cortical granules behave differently in mouse oocytes matured under different conditions. | to better understand the differences between in vivo (ivo) and in vitro (ivm) matured oocytes, we studied the chronological changes in cortical granule (cg) distribution and nuclear progression during maturation, and the competence of cg release and embryo development of mouse oocytes matured under different conditions. | 2005 | 16172151 |
| lectins as bioactive plant proteins: a potential in cancer treatment. | plant lectins, a unique group of proteins and glycoproteins with potent biological activity, occur in foods like wheat, corn, tomato, peanut, kidney bean, banana, pea, lentil, soybean, mushroom, rice, and potato. thus, dietary intakes by humans can be significant. many lectins resist digestion, survive gut passage, and bind to gastrointestinal cells and/or enter the circulation intact, maintaining full biological activity. several lectins have been found to possess anticancer properties in vitro ... | 2005 | 16183566 |
| serum kl-6 as a novel tumor marker for hepatocellular carcinoma in hepatitis c virus infected patients. | the up-regulation of muc1 protein is associated with malignant phenotype of cancer. we investigated the significance of kl-6, one of the muc1 antigens, as a tumor marker in hepatitis c virus positive hepatocellular carcinoma (hcc). serum kl-6 was determined in 203 patients with chronic hepatitis (ch), 47 patients with liver cirrhosis (lc) and 78 patients with hcc. kl-6 was higher in hcc compared to non-hcc (p=0.0005) and was higher in patients with multiple hcc nodules compared to a single nodul ... | 2005 | 16202647 |
| serodiagnosis of neurocysticercosis using synthetic 8-kd proteins: comparison of assay formats. | the assay of choice for serological detection of cysticercosis in humans and pigs is the enzyme-linked immunoelectrotransfer blot (eitb), a western blot assay that relies on the use of seven lentil-lectin-purified glycoproteins (llgps) derived from taenia solium metacestodes. the eitb is has a sensitivity of 98% and a specificity of 100% in detecting cysticercosis, yet scarcity of native source material and the labor-intensive process of metacestode purification hinder its practicality. these li ... | 2005 | 16222024 |
| a provisional database for the silicon content of foods in the united kingdom. | si may play an important role in bone formation and connective tissue metabolism. although biological interest in this element has recently increased, limited literature exists on the si content of foods. to further our knowledge and understanding of the relationship between dietary si and human health, a reliable food composition database, relevant for the uk population, is required. a total of 207 foods and beverages, commonly consumed in the uk, were analysed for si content. composite samples ... | 2005 | 16277785 |
| colorimetric enzyme-linked immunosorbent assay for the identification of strains of rhizobium in culture and in the nodules of lentils. | an indirect enzyme-linked immunosorbent assay has been developed to identify strains of rhizobium in culture and in lentil nodules. the test can be used on cells from both fresh and frozen nodules obtained from plants grown either in a growth chamber or in the field. test results were confirmed by immunofluorescence. the enzyme-linked immunosorbent assay technique can be used for field studies and requires less antisera than other serological techniques. | 1979 | 16345362 |
| competition among rhizobium leguminosarum strains for nodulation of lentils (lens esculenta). | thirty-one cultures of rhizobium leguminosarum were screened for effectiveness (c(2)h(2) reduction) on lentils (lens esculenta). fluorescent antibodies prepared against three of the most effective strains (hawaii 5-0, nitragin 92a3, and nitragin 128a12) exhibited a high degree of strain specificity; the antibodies reacted strongly with their homologous rhizobia in culture and with bacteroids in nodules. they did not cross-react with one another, and only weakly with 5 of the 47 other r. legumino ... | 1983 | 16346257 |
| an n-glycan structure correlates with pulmonary metastatic ability of cancer cells. | n-glycan structures on the surface of cancer cells have diverse structures and play significant roles in metastatic process. however, little is known about their roles in organ-selective metastasis. our study revealed that an alpha1,6-fucosylated biantennary n-glycan structure designated a2g2f is characteristic of lungs, with far more abundant expression in normal human and murine lungs than in other organs. in this study, we further examined the role of a2g2f in pulmonary metastasis. we stained ... | 2006 | 16380076 |
| analysis of unconventional approaches for the rapid detection of surface lectin binding ligands on human cell lines. | for over a decade our laboratory has developed and used a novel histochemical assay using derivatized agarose beads to examine the surface properties of various cell types. most recently, we have used this assay to examine lectin binding ligands on two human cell types, ccl-220, a colon cancer cell line, and crl-1459, a non-cancer colon cell line. we found that ccl-220 cells bound specific lectins better than crl-1459, and this information was used to test for possible differential toxicity of t ... | 2006 | 16414103 |
| three new species of eimeria (apicomplexa: eimeriidae) from the silvery mole rat heliophobius argenteocinereus peters, 1846 (rodentia: bathyergidae) from malawi. | three species of eimeria schneider are described from feces of the african bathyergid rodent, heliophobius argenteocinereus, from malawi. oocysts of eimeria heliophobii n. sp. are broadly ellipsoidal; 27.9 (22-31) x 22.3 (18-24.5) microm with a brownish, heavily pitted oocyst wall, and vacuolar oocyst residuum. sporocysts are oval, 12.8 (12-14) x 8.4 (8-9) microm with stieda and substieda bodies. eimeria nafuko n. sp. has subspherical oocysts; 15.5 (15-16) x 12.8 (12-13) microm with a smooth, co ... | 2005 | 16419767 |
| dual expression of the ha protein of h5n2 avian influenza virus in a baculovirus system. | baculovirus/insect cell system is used widely for recombinant protein production. the hemagglutinin (ha) gene of h5n2 avian influenza virus (aiv) 1209 strain and the enhanced green fluorescent protein (egfp) gene were cloned into pfastbac dual vector that has two promoters and cloning sites, allowing simultaneous expression of these two genes. the ha protein of aiv was fused with a hexahistidine (his6) tag for purification. the coexpression of egfp allowed identification of the recombinant bacul ... | 2006 | 16530857 |
| characterization and cloning of t24, a taenia solium antigen diagnostic for cysticercosis. | the third and final diagnostic antigen of the lentil lectin purified glycoproteins (llgp) extracted from the larval stage of taenia solium has been characterized, cloned, and expressed. t24 is an integral membrane protein that belongs to the tetraspanin superfamily. it migrates at a position corresponding to 24-kda and as a homodimer at 42-kda. antibodies from cysticercosis patients recognize secondary structure epitopes that are dependent upon correctly formed disulfide bonds. a portion of t24, ... | 2006 | 16540186 |
| use of inexpensive nitrogen sources and starch for l(+) lactic acid production in anaerobic submerged fermentation. | l(+) lactic acid fermentation was studied by lactobacillus amylophilus gv6 under the influence of inexpensive nitrogen sources (red lentil-rl, and baker's yeast cells-yc) and starch by response surface methodology (rsm). central composite rotatable design (ccrd) was employed to determine maximum lactic acid production at optimum values for process variables rl, yc and incubation period (ip) and a satisfactory fit model was realized. lactic acid production was significantly affected by rl and ip ... | 2007 | 16563750 |
| comparison of cell lines for stable production of fucose-negative antibodies with enhanced adcc. | several methods have been described to enhance antibody-dependent cellular cytotoxicity (adcc) using different host cells that produce antibody with reduced levels of fucose on their carbohydrates. we compared the suitability of these methods for the serum-free fed-batch production of antibody for clinical trials and commercial uses. recombinant anti-human cd20 chimeric igg1-producing clones were established from host-cells that have been shown to produce more than 90% fucose-negative antibody. ... | 2006 | 16609957 |
| interactions between rhizobia and lectins of lentil, pea, broad bean, and jackbean. | a quantitative method was developed to measure the binding of fluorescent-labeled lentil (lens esculenta moench), pea (pisum sativum l.), broad bean (vicia faba l.), and jackbean (canavalia ensiformis l., dc.) lectins to various rhizobium strains. lentil lectin bound to three of the five rhizobium leguminosarum strains tested. the number of lentil lectin molecules bound per r. leguminosarum 128c53 cell was 2.1 x 10(4). lentil lectin also bound to r. japonicum 61a133. pea and broad bean lectins b ... | 1980 | 16661328 |
| determination of diamine oxidase in lentil seedlings by enzymic activity and immunoreactivity. | a competitive radioimmunoassay for the quantitation of diamine oxidase (ec 1.4.3.6) from lens culinaris is reported. specific antibodies raised in rabbits immunized with a homogeneous preparation of the enzyme were incubated with purified (125)i-enzyme and with either unlabeled diamine oxidase or plant material. antigen-antibody complexes were isolated from the mixture by incubation with staphylococcus protein a. the sensitivity of the test was about 5 nanograms in terms of enzyme protein. this ... | 1985 | 16664402 |
| cloning of the mating type locus from ascochyta lentis (teleomorph: didymella lentis) and development of a multiplex pcr mating assay for ascochyta species. | the mating type (mat) locus of the lentil pathogen, ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse pcr with primers designed to the hmg-box of ascochyta rabiei. a multiplex pcr assay for mating type was developed based on mat idiomorph and flanking sequences. primers were designed to specifically amplify mat from several ascochyta spp. including a. pisi, a. fabae and a. viciae-villosae in addition to a. lentis. four hundred and fifty and 700 bp fra ... | 2006 | 16847660 |
| role of roscovitine and ibmx on kinetics of nuclear and cytoplasmic maturation of bovine oocytes in vitro. | the 3-isobutyl-1-methylxanthine (ibmx) is able to prevent resumption of meiosis by maintaining elevated cyclic amp (camp) concentrations in the oocyte, and roscovitine, a purine known to specifically inhibit mpf kinase activity, maintains bovine oocytes at the germinal vesicle (gv) stage. the present study was conducted to analyze whether cytoplasmic maturation (examined by the pattern of cortical granule (cg) distribution) of bovine oocytes is improved during meiotic arrest with ibmx and roscov ... | 2007 | 16860950 |
| glomerella truncata sp. nov., the teleomorph of colletotrichum truncatum. | anthracnose of lentil, caused by colletotrichum truncatum is a serious threat to lentil (lens culinaris) grown in western canada. the teleomorph stage of this pathogen was induced to form under laboratory conditions. random pairing of single conidium isolates enabled the identification of fertile isolates. the individual isolates of this fertile pair were crossed with 14 other isolates, and all isolates were also incubated alone. self-sterility was observed for all 16 isolates tested. three isol ... | 2006 | 16895753 |
| allergic hypersensitivity to the lentil pest bruchus lentis. | lentils are the most common legume involved in allergic reactions in the mediterranean area and india. lentil crops could be attacked by a wide range of insect species. occupational asthma caused by the lentil pest bruchus lentis has been described in the present study. | 2006 | 16918515 |
| in situ study of the relevance of bacterial adherence to feed particles for the contamination and accuracy of rumen degradability estimates for feeds of vegetable origin. | an in situ study was conducted on four rumen-cannulated wethers to determine (using (15)n infusion techniques) the microbial contamination (mg bacterial dm or crude protein (cp)/100 mg dm or cp) and the associated error on the effective degradability of fourteen feeds: barley and maize grains, soyabean and sunflower meals, full-fat soyabean, maize gluten feed, soyabean hulls, brewers dried grains, sugarbeet pulp, wheat bran, lucerne and vetch-oat hays, and barley and lentil straws. the dm or cp ... | 2006 | 16923226 |
| determination of iodine in libyan food samples using epithermal instrumental neutron activation analysis. | epithermal instrumental neutron activation analysis (einaa) has been used to determine the iodine content of many individual food materials that constitute the typical libyan diet. the selected samples include different varieties of local and imported foods such as wheat and barley products, rice, bread, legumes such as chick peas and lentil, table salt, and commonly used spices, including thyme and fenugreek. both conventional and anticoincidence gamma-ray spectrometry techniques have been empl ... | 2006 | 16943595 |
| polyketone polymer: a new support for direct enzyme immobilization. | polyketone polymer -[-co-ch(2)-ch(2)-](n)-, obtained by copolymerization of ethene and carbon monoxide, is utilized for immobilization of three different enzymes, one peroxidase from horseradish (hrp) and two amine oxidases, from bovine serum (bsao) and lentil seedlings (lsao). the easy immobilization procedure is carried out in diluted buffer, at ph 7.0 and 3 degrees c, gently mixing the proteins with the polymer. no bifunctional reagents and spacer arms are required for the immobilization, whi ... | 2007 | 17007953 |
| analysis of differential scanning calorimetry data for proteins. criteria of validity of one-step mechanism of irreversible protein denaturation. | we consider in this work the analysis of the excess heat capacity c(p)(ex) versus temperature profiles in terms of a model of thermal protein denaturation involving one irreversible step. it is shown that the dependences of ln c(p)(ex) on 1 t (t is the absolute temperature) obtained at various temperature scanning rates have the same form. several new methods for estimation of parameters of the arrhenius equation are explored. these new methods are based on the fitting of theoretical equations t ... | 1997 | 17029925 |
| serum markers of hepatocellular carcinoma. | hepatocellular carcinoma (hcc) is one of the most common malignant tumors in some areas of the world with increasing incidence worldwide. most of patients with hcc are diagnosed at a late stage. therefore, the prognosis of hcc patients is generally very poor with a 5-year survival rate of less than 5%. screening strategies including alpha-fetoprotein (afp) and ultrasound every 6 months in patients with liver cirrhosis, the major risk factor for hcc development, have been recommended to detect hc ... | 2006 | 17051452 |
| an antifungal peptide from red lentil seeds. | an antifungal peptide, with a molecular mass of 11 kda, was isolated from dry seeds of the red lentil (lens culinaris) using a procedure that involved four chromatographic steps. the antifungal peptide was unadsorbed on deae-cellulose, and adsorbed on affi-gel blue gel and s-sepharose. the final chromatographic step involved gel filtration by fast protein liquid chromatography on superdex 75. the antifungal peptide inhibited mycelial growth in mycosphaerella arachidicola with an ic50 of 36 micro ... | 2007 | 17123664 |
| staging hepatocellular carcinoma by a novel scoring system (balad score) based on serum markers. | previously proposed staging systems for hepatocellular carcinoma (hcc) involve clinical, imaging, or pathologic factors in the evaluation. we established and validated a novel staging system for hcc that is based on simply serum markers. | 2006 | 17162244 |
| conformation-sensitive immunoassays improve the serodiagnosis of solitary cysticercus granuloma in indian patients. | neurocysticercosis (ncc), infection of the central nervous system with larva of taenia solium, presents in over 60% of patients in india as a solitary cysticercus granuloma (scg). the low cyst number in these patients frequently results in an insignificant humoral response. consequently, serological tests for patients with scg must consider the detection of low antibody levels. lentil lectin-specific t. solium glycoproteins of molecular weights 50, 38, 24, 18, 14 and 13 kda are specific antigens ... | 2007 | 17169388 |
| the clinical utility of lens culinaris agglutinin-reactive thyroglobulin ratio in serum for distinguishing benign from malignant conditions of the thyroid. | traditionally, the follow-up of differentiated thyroid carcinoma consists of periodic withdrawal from l-t4-suppressive therapy to allow performance of a highly sensitive serum tg measurement to detect recurrences. we investigated lens culinaris agglutinin-reactive thyroglobulin ratios in serum to evaluate in usefulness for detection of thyroid carcinoma. | 2007 | 17270168 |
| expression profiling of chickpea genes differentially regulated during a resistance response to ascochyta rabiei. | using microarray technology and a set of chickpea (cicer arietinum l.) unigenes, grasspea (lathyrus sativus l.) expressed sequence tags (ests) and lentil (lens culinaris med.) resistance gene analogues, the ascochyta blight (ascochyta rabiei (pass.) l.) resistance response was studied in four chickpea genotypes, including resistant, moderately resistant, susceptible and wild relative (cicer echinospermum l.) genotypes. the experimental system minimized environmental effects and was conducted in ... | 2006 | 17309735 |
| gonadotropin-like and adrenocorticotropin-like cells in the pituitary gland of hagfish, paramyxine atami; immunohistochemistry in combination with lectin histochemistry. | the pituitary system of the hagfish remains an enigma. the present study has aimed to detect possible adenohypophysial hormones in the pituitary gland of the brown hagfish, paramyxine atami, by means of immunohistochemistry in combination with lectin histochemistry. rabbit antisera raised against ovine luteinizing hormone (lh)beta, proopiomelanocortin (pomc)-related peptides, and the growth hormone/prolactin family of tetrapod and fish species were used, and 25 kinds of lectins were tested. thre ... | 2007 | 17347815 |
| relevance of serum ige estimation in allergic bronchial asthma with special reference to food allergy. | studies suggest the importance of serum total and specific ige in clinical evaluation of allergic manifestations. such studies are lacking in indian subcontinent, though a large population suffer from bronchial asthma. here relevance of serum total and specific ige was investigated in asthmatics with food sensitization. a total of 216 consecutive patients (mean age 31.9 years, s.d. 11.8) were screened by various diagnostic testing. out of 216 patients, 172 were with elevated serum total ige (201 ... | 2006 | 17348241 |
| lectin binding profile of the small intestine of five-week-old pigs in response to the use of chlortetracycline as a growth promotant and under gnotobiotic conditions. | antibiotics have traditionally been used for growth promotion in the pork industry; however, their use in animal feed has recently been limited because of human health concerns. the intestinal microbiota plays an important role in mediating many physiological functions such as digestion and animal growth. it was hypothesized that use of antibiotics as growth promotants and subsequent variations in intestinal microbiota induce significant changes in the intestinal glycoconjugate composition, whic ... | 2007 | 17400973 |
| an important lysine residue in copper/quinone-containing amine oxidases. | the interaction of xenon with copper/6-hydroxydopa (2,4,5-trihydroxyphenethylamine) quinone (tpq) amine oxidases from the plant pulses lentil (lens esculenta) and pea (pisum sativum) (seedlings), the perennial mediterranean shrub euphorbia characias (latex), and the mammals cattle (serum) and pigs (kidney), were investigated by nmr and optical spectroscopy of the aqueous solutions of the enzymes. (129)xe chemical shift provided evidence of xenon binding to one or more cavities of all these enzym ... | 2007 | 17433047 |