| new features of triacylglycerol biosynthetic pathways of peanut seeds in early developmental stages. | the peanut (arachis hypogaea l.) is one of the three most important oil crops in the world due to its high average oil content (50 %). to reveal the biosynthetic pathways of seed oil in the early developmental stages of peanut pods with the goal of improving the oil quality, we presented a method combining deep sequencing analysis of the peanut pod transcriptome and quantitative real-time pcr (rt-pcr) verification of seed oil-related genes. from the sequencing data, approximately 1500 lipid meta ... | 2015 | 26071211 |
| global synthesis of drought effects on food legume production. | food legume crops play important roles in conservation farming systems and contribute to food security in the developing world. however, in many regions of the world, their production has been adversely affected by drought. although water scarcity is a severe abiotic constraint of legume crops productivity, it remains unclear how the effects of drought co-vary with legume species, soil texture, agroclimatic region, and drought timing. to address these uncertainties, we collected literature data ... | 2015 | 26061704 |
| o-glycosylation of α-1-acid glycoprotein of human milk is lactation stage related. | human milk provides a multitude of glycoproteins, including highly glycosylated α-1-acid glycoprotein (agp), which elicits anti-inflammatory and immunomodulatory properties. the milk agp glycoforms may provide the breastfed infant with a wide range of biological benefits. here, we analyzed the reactivity of o-linked sugar-specific lectins with human milk agp over the process of lactation and compared the results with those of the lactating mother's plasma. | 2015 | 26057552 |
| tillage impact on herbicide loss by surface runoff and lateral subsurface flow. | there is worldwide interest in conservation tillage practices because they can reduce surface runoff, and agrichemical and sediment losses from farm fields. since these practices typically increase infiltration, their use may increase subsurface transport of water-soluble contaminants. thus, to assess long-term environmental benefits of conservation tillage data may be needed that quantify both surface and subsurface contaminant fluxes. this study focused on the herbicide fluometuron (n,n-dimeth ... | 2015 | 26057540 |
| novel and stress relevant est derived ssr markers developed and validated in peanut. | with the aim to increase the number of functional markers in resource poor crop like cultivated peanut (arachis hypogaea), large numbers of available expressed sequence tags (ests) in the public databases, were employed for the development of novel est derived simple sequence repeat (ssr) markers. from 16424 unigenes, 2784 (16.95%) ssrs containing unigenes having 3373 ssr motifs were identified. of these, 2027 (72.81%) sequences were annotated and 4124 gene ontology terms were assigned. among di ... | 2015 | 26046991 |
| effect of feeding lipids recovered from fish processing waste by lactic acid fermentation and enzymatic hydrolysis on antioxidant and membrane bound enzymes in rats. | fish oil recovered from fresh water fish visceral waste (fvw-fo) through lactic acid fermentation (fo-laf) and enzymatic hydrolysis (fo-eh) were fed to rats to study their influence on lipid peroxidation and activities of antioxidant and membrane bound enzyme in liver, heart and brain. feeding of fo-laf and fo-eh resulted in increase (p < 0.05) in lipid peroxides level in serum, liver, brain and heart tissues compared to ground nut oil (control). activity of catalase (40-235 %) and superoxide di ... | 2015 | 26028754 |
| rhizobium anhuiense sp. nov., isolated from effective nodules of vicia faba and pisum sativum. | four rhizobia-like strains, isolated from root nodules of pisum sativum and vicia faba grown in anhui and jiangxi provinces of china, were grouped into the genus rhizobium but were distinct from all recognized species of the genus rhizobium by phylogenetic analysis of 16s rrna and housekeeping genes. the combined sequences of the housekeeping genes atpd, reca and glnii for strain ccbau 23252(t) showed 86.9 to 95% similarity to those of known species of the genus rhizobium. all four strains had n ... | 2015 | 26025940 |
| influence of the insecticides acetamiprid and carbofuran on arylamidase and myrosinase activities in the tropical black and red clay soils. | the objective of this study was to determine the effects of two insecticides, namely, acetamiprid and carbofuran on the enzymatic activities of arylamidase (as glucose formed from sinigrin) and myrosinase (as β-naphthylamine formed from l-leucine β-naphthylamide) in the black and red clay soils collected from a fallow groundnut (arachis hypogaea l.) fields in the anantapur district, andhra pradesh, india. the study was realized within the framework of the laboratory experiments in which the acet ... | 2015 | 26024750 |
| growth analysis and yield of two varieties of groundnut (arachis hypogaea l.) as influenced by different weed control methods. | field trials were carried out to evaluate the effects of seven weed management strategies on the growth and yield of two groundnut varieties (samnut 10 and mk 373) for two successive seasons (2010-2011). the experimental layout was a split plot complete randomized block design with three replications. the two groundnut varieties showed identical pattern of results for leaf area index, dry matter accumulation, relative growth rate, net assimilation rate and crop growth rate as well as yield. all ... | 2016 | 26005217 |
| negative biomarker based male fertility evaluation: sperm phenotypes associated with molecular-level anomalies. | biomarker-based sperm analysis elevates the treatment of human infertility and ameliorates reproductive performance in livestock. the negative biomarker-based approach focuses on proteins and ligands unique to defective spermatozoa, regardless of their morphological phenotype, lending itself to analysis by flow cytometry (fc). a prime example is the spermatid specific thioredoxin sptrx3/txndc8, retained in the nuclear vacuoles and superfluous cytoplasm of defective human spermatozoa. infertile c ... | 2016 | 25999356 |
| molecular cloning and characterization of annexin genes in peanut (arachis hypogaea l.). | annexin, ca(2+) or phospholipid binding proteins, with many family members are distributed throughout all tissues during plant growth and development. annexins participate in a number of physiological processes, such as exocytosis, cell elongation, nodule formation in legumes, maturation and stress response. six different full-length cdnas and two partial-length cdnas of peanut, (annah1, annah2, annah3, annah5, annah6, annah7, annah4 and annah8) encoding annexin proteins, were isolated and chara ... | 2015 | 25958350 |
| peanut violaxanthin de-epoxidase alleviates the sensitivity of psii photoinhibition to heat and high irradiance stress in transgenic tobacco. | this is the first study on peanut vde, which led to multiple biochemical and physiological changes to heat and hi stress by improving de-epoxidation of the xanthophylls cycle. a peanut (arachis hypogaea l.) violaxanthin de-epoxidase gene (ahvde) was isolated by rt-pcr and race methods. the deduced amino acid sequence of ahvde showed high identities with violaxanthin de-epoxidase of other plant species. the expression of ahvde was obviously upregulated by 4, 40 °c and high light, nacl, and abscis ... | 2015 | 25916178 |
| tilling by sequencing to identify induced mutations in stress resistance genes of peanut (arachis hypogaea). | targeting induced local lesions in genomes (tilling) is a powerful reverse genetics approach for functional genomics studies. we used high-throughput sequencing, combined with a two-dimensional pooling strategy, with either minimum read percentage with non-reference nucleotide or minimum variance multiplier as mutation prediction parameters, to detect genes related to abiotic and biotic stress resistances. in peanut, lipoxygenase genes were reported to be highly induced in mature seeds infected ... | 2015 | 25881128 |
| synergic actions of polyphenols and cyanogens of peanut seed coat (arachis hypogaea) on cytological, biochemical and functional changes in thyroid. | in animals, long-term feeding with peanut (arachis hypogaea) seed coats causes hypertrophy and hyperplasia of the thyroid gland. however, to date there have been no detailed studies. here, we explored the thyroidal effects of dietary peanut seed coats (psc) in rats. the psc has high levels of pro-goitrogenic substances including phenolic and other cyanogenic constituents. the psc was mixed with a standard diet and fed to rats for 30 and 60 days, respectively. animals fed with the psc-supplemente ... | 2015 | 25872244 |
| cloning and characterization of peanut allene oxide cyclase gene involved in salt-stressed responses. | in this study, the full-length cdna encoding allene oxide cyclase (ahaoc) was isolated from peanut (arachis hypogaea l.). the deduced amino acid sequence of ahaoc showed high homology with other plant aocs. the transcript of ahaoc was found to be abundantly expressed in roots. expression analysis demonstrated that ahaoc was induced by abscisic acid, methyl-jasmonic acid, salicylic acid, salinity, polyethylene glycol, and cold stresses, particularly by high salinity. overexpression of ahaoc in ri ... | 2015 | 25867379 |
| chryseobacterium arachidiradicis sp. nov., isolated from the geocarposphere (soil around the peanut) of very immature peanuts (arachis hypogaea). | a yellow-pigmented bacterial strain, 91a-612(t), isolated from the geocarposphere (soil around the peanut) of very immature peanuts (arachis hypogaea) in alabama, usa, was studied for its taxonomic position. cells of the isolate were rod-shaped and stained gram-negative. a comparison of the 16s rrna gene sequence with the sequences of the type strains of the most closely related species showed that the strain belongs to the genus chryseobacterium, showing the highest sequence similarities to the ... | 2015 | 25858249 |
| genetic mapping of qtls controlling fatty acids provided insights into the genetic control of fatty acid synthesis pathway in peanut (arachis hypogaea l.). | peanut, a high-oil crop with about 50% oil content, is either crushed for oil or used as edible products. fatty acid composition determines the oil quality which has high relevance to consumer health, flavor, and shelf life of commercial products. in addition to the major fatty acids, oleic acid (c18:1) and linoleic acid (c18:2) accounting for about 80% of peanut oil, the six other fatty acids namely palmitic acid (c16:0), stearic acid (c18:0), arachidic acid (c20:0), gadoleic acid (c20:1), behe ... | 2015 | 25849082 |
| spatial and temporal dynamics of stink bugs in southeastern farmscapes. | a 3-yr study (2009-2011) was conducted to examine the spatial and temporal dynamics of stink bugs in three commercial farmscapes. study locations were replicated in south carolina and georgia, in an agriculturally diverse region known as the southeastern coastal plain. crops included wheat, triticum aestivum (l.), corn, zea mays (l.), soybean, glycine max (l.), cotton, gossypium hirsutum (l.), and peanut, arachis hypogaea (l.). farmscapes were sampled weekly using whole-plant examinations for co ... | 2015 | 25843577 |
| enhanced production of resveratrol, piceatannol, arachidin-1, and arachidin-3 in hairy root cultures of peanut co-treated with methyl jasmonate and cyclodextrin. | peanut (arachis hypogaea) produces stilbenoids upon exposure to abiotic and biotic stresses. among these compounds, the prenylated stilbenoids arachidin-1 and arachidin-3 have shown diverse biological activities with potential applications in human health. these compounds exhibit higher or novel biological activities in vitro when compared to their nonprenylated analogues piceatannol and resveratrol, respectively. however, assessment of these bioactivities in vivo has been challenging because of ... | 2015 | 25837778 |
| struvite for composting of agricultural wastes with termite mound: utilizing the unutilized. | although, compost is the store house of different plant nutrients, there is a concern for low amount of major nutrients especially nitrogen content in prepared compost. the present study deals with preparation of compost by using agricultural wastes with struvite (mgnh4po4·6h2o) along with termite mound. among four composting mixtures, 50kg termite mound and 2.5kg struvite with crop residues (stover of ground nut: 361.65kg; soybean: 354.59kg; potato: 357.67kg and mustard: 373.19kg) and cow dung ... | 2015 | 25836374 |
| quantitative trait locus analysis of agronomic and quality-related traits in cultivated peanut (arachis hypogaea l.). | ssr-based qtl mapping provides useful information for map-based cloning of major qtls and can be used to improve the agronomic and quality traits in cultivated peanut by marker-assisted selection. cultivated peanut (arachis hypogaea l.) is an allotetraploid species (aabb, 2n = 4× = 40), valued for its edible oil and digestible protein. linkage mapping has been successfully conducted for most crops, and it has been applied to detect the quantitative trait loci (qtls) of biotic and abiotic traits ... | 2015 | 25805315 |
| sequence and expression analysis of putative arachis hypogaea (peanut) nod factor perception proteins. | peanut, like most legumes, develops a symbiotic relationship with rhizobia to overcome nitrogen limitation. rhizobial infection of peanut roots occurs through a primitive and poorly characterized intercellular mechanism. knowledge of the molecular determinants of this symbiotic interaction is scarce, and little is known about the molecules implicated in the recognition of the symbionts. here, we identify the lysm extracellular domain sequences of two putative peanut nod factor receptors, named a ... | 2015 | 25801275 |
| [fourier transform infrared spectral analysis on peanut (arachis hypogaea) plants under calcium deficiency stress]. | the objective of the present study was to reveal different tolerance of peanut plants to ca deficiency by determining ca uptake and fourier transform infrared spectral (ftir) differences of two peanut cultivars grown in nutrition solution. peanut cultivars lh11 and yz9102 were selected. seedlings at the first leaf stage were cultivated for 28 days in nutrient solution with 0, 0.01 and 2.0 mmol x l(-1) ca treatments, respectively. the results showed that under 0 and 0.01 mmol x l(-1) ca supply, y ... | 2014 | 25752032 |
| reduction of platelet aggregation from ingestion of oleic and linoleic acids found in vitis vinifera and arachis hypogaea oils. | the purpose of this study was to evaluate the effect of the consumption of seed oils from vitis vinifera and arachis hypogaea in platelet aggregation. the initial hypothesis suggested that subjects who have consumed these seed oils undergo modified platelet aggregation. this study was performed using a pre-post test design, with a control group, and double blind. the effects of the consumption of grape seed and peanut oils were measured for platelet aggregation in clinical and laboratory tests i ... | 2017 | 25741817 |
| an economical and efficient technology for the extraction of resveratrol from peanut (arachis hypogaea) sprouts by multi-stage countercurrent extraction. | in this paper, an economical and efficient technology for the extraction of resveratrol from peanut sprouts by multi-stage countercurrent extraction (msce) was investigated based on the alkaline extraction and acid precipitation method (aeap). firstly, the msce equipment and operation procedures were designed. then, the optimal parameters of msce were obtained by using single-factor experiments and box-behnken design (bbd) as follows: extraction temperature of 46.6 °c, cao to raw material ratio ... | 2015 | 25722134 |
| overexpression of cuznsod from arachis hypogaea alleviates salinity and drought stress in tobacco. | overexpression of cuznsod gene from arachis hypogaea demonstrating its involvement in abiotic stress tolerance. abiotic stress is accompanied by the formation of reactive oxygen species (ros) such as superoxide, hydrogen peroxide, and hydroxyl radicals, causing extensive cellular damage and inhibition of photosynthesis that limit the plant productivity. the level of ros in cells needs to be tightly regulated and the toxic effects of ros are countered by enzymatic as well as non-enzymatic antioxi ... | 2015 | 25712013 |
| tetrasomic recombination is surprisingly frequent in allotetraploid arachis. | arachis hypogaea l. (cultivated peanut) is an allotetraploid (2n = 4x = 40) with an aabb genome type. based on cytogenetic studies it has been assumed that peanut and wild-derived induced aabb allotetraploids have classic allotetraploid genetic behavior with diploid-like disomic recombination only between homologous chromosomes, at the exclusion of recombination between homeologous chromosomes. using this assumption, numerous linkage map and quantitative trait loci studies have been carried out. ... | 2015 | 25701284 |
| next-generation transcriptome sequencing, snp discovery and validation in four market classes of peanut, arachis hypogaea l. | single-nucleotide polymorphisms, which can be identified in the thousands or millions from comparisons of transcriptome or genome sequences, are ideally suited for making high-resolution genetic maps, investigating population evolutionary history, and discovering marker-trait linkages. despite significant results from their use in human genetics, progress in identification and use in plants, and particularly polyploid plants, has lagged. as part of a long-term project to identify and use snps su ... | 2015 | 25663138 |
| generation of peanut mutants by fast neutron irradiation combined with in vitro culture. | induced mutations have played an important role in the development of new plant varieties. in this study, we investigated the effects of fast neutron irradiation on somatic embryogenesis combined with plant regeneration in embryonic leaflet culture to develop new peanut (arachis hypogaea l.) germplasm for breeding. the dry seeds of the elite cultivar luhua 11 were irradiated with fast neutrons at dosages of 9.7, 14.0 and 18.0 gy. the embryonic leaflets were separated and incubated in a medium wi ... | 2015 | 25653418 |
| mapping b-cell epitopes of major and minor peanut allergens and identifying residues contributing to ige binding. | epitope identification provides valuable information essential for understanding antigen components involved in food allergic reactions. in the present study, an in silico approach is employed to map ige binding epitopes of major and minor peanut allergens. | 2016 | 25652191 |
| removal of peanut allergen ara h 1 from common hospital surfaces, toys and books using standard cleaning methods. | in children, a diagnosis of peanut allergy causes concern about accidental exposure because even small amounts of peanut protein could trigger an allergic reaction. contamination of toys, books or other items by peanut butter in areas where individuals have eaten may occur in hospital waiting rooms and cafeterias. it is not known if hospital cleaning wipes are effective in removing peanut allergen. | 2015 | 25642252 |
| stability of transgene expression in reduced allergen peanut (arachis hypogaea l.) across multiple generations and at different soil sulfur levels. | transgenic peanut (arachis hypogaea l.) containing a gene designed for rna interference (rnai) showed stable complete silencing of ara h 2 and partial silencing of ara h 6, two potent peanut allergens/proteins, along with minimal collateral changes to other allergens, ara h 1 and ara h 3, across three generations (t3, t4, and t5) under field conditions. different soil sulfur levels (0.012, 0.3, and 3.0 mm) differentially impacted sulfur-rich (ara h 2, ara h 3, and ara h 6) versus sulfur-poor (ar ... | 2015 | 25616282 |
| molecular cloning, expression, and evolution analysis of type ii chi gene from peanut (arachis hypogaea l.). | chalcone isomerase (chi) plays critical roles in plant secondary metabolism, which is important for the interaction between plants and the environment. chi genes are widely studied in various higher plants. however, little information about chi genes is available in peanut. based on conservation of chi gene family, we cloned the peanut type ii chi gene (ahchi ii) cdna and genome sequence. the amino acid sequence of peanut chi ii was highly homologous to type ii chi from other plant species. qrt- ... | 2015 | 25608978 |
| ocsestdb: a database of oil crop seed est sequences for comparative analysis and investigation of a global metabolic network and oil accumulation metabolism. | oil crop seeds are important sources of fatty acids (fas) for human and animal nutrition. despite their importance, there is a lack of an essential bioinformatics resource on gene transcription of oil crops from a comparative perspective. in this study, we developed ocsestdb, the first database of expressed sequence tag (est) information on seeds of four large-scale oil crops with an emphasis on global metabolic networks and oil accumulation metabolism that target the involved unigenes. | 2015 | 25604238 |
| comparisons of de novo transcriptome assemblers in diploid and polyploid species using peanut (arachis spp.) rna-seq data. | the narrow genetic base and limited genetic information on arachis species have hindered the process of marker-assisted selection of peanut cultivars. however, recent developments in sequencing technologies have expanded opportunities to exploit genetic resources, and at lower cost. to use the genetic information for arachis species available at the transcriptome level, it is important to have a good quality reference transcriptome. the available tifrunner 454 flex transcriptome sequences have a ... | 2014 | 25551607 |
| influence of insecticides flubendiamide and spinosad on biological activities in tropical black and red clay soils. | a laboratory experiment has been conducted to investigate the ecological toxicity of flubendiamide and spinosad at their recommended field rates and higher rates (1.0, 2.5, 5.0, 7.5, 10.0 kg ha(-1)) on cellulase, invertase and amylase in black and red clay soils after 10, 20, 30 and 40-day exposure under controlled conditions in groundnut (arachis hypogaea l.) soils of anantapur district, andhra pradesh, india. flubendiamide and spinosad were stimulatory to the activities of cellulase, invertase ... | 2015 | 28324354 |
| heterologous expression of the atdreb1a gene in transgenic peanut-conferred tolerance to drought and salinity stresses. | research on genetic transformation in various crop plants using the dreb1a transcription factor has shown better abiotic stress tolerance in transgenic crops. the atdreb1a transgenic peanut (arachis hypogaea l. cv. gg 20), which was previously developed, was characterized in terms of its physio-biochemical, molecular and growth parameters. the tolerance of this transgenic peanut to drought and salinity stresses was evaluated at the seedling (18 days old) and maturity stages. transgenic peanut li ... | 2014 | 25545786 |
| ph-induced conformational isomerization of leghemoglobin from arachis hypogea. | the ph dependence of proteins is related to the thermodynamic stability and electrostatic interactions in the native state of a protein. here we report the ph-induced conformational transition of the heme protein leghemoglobin (lb) isolated from root nodules of the leguminous plant arachis hypogea. unlike the other heme proteins myoglobin, hemoglobin, and cytochrome c, the structural characteristics and interactions of lb is almost unknown, though its functional importance is already established ... | 2014 | 25540011 |
| detection of peanut (arachis hypogaea) allergen by real-time pcr method with internal amplification control. | specific primer sets were designed based on the dna sequence of ara h 1, one of the major peanut (arachis hypogaea) allergens, and a competitive internal amplification control (iac) was designed by compound primer technology. by choosing 314 copies/pcr as the iac dosage, a real-time pcr method with iac was established for detecting peanut allergen ara h 1 dna. the method showed high specificity with a detection limit of 0.005% peanut. a series of commercial food products with/without peanut comp ... | 2015 | 25529717 |
| identification of qtls associated with oil content and mapping fad2 genes and their relative contribution to oil quality in peanut (arachis hypogaea l.). | peanut is one of the major source for human consumption worldwide and its seed contain approximately 50% oil. improvement of oil content and quality traits (high oleic and low linoleic acid) in peanut could be accelerated by exploiting linked markers through molecular breeding. the objective of this study was to identify qtls associated with oil content, and estimate relative contribution of fad2 genes (ahfad2a and ahfad2b) to oil quality traits in two recombinant inbred line (ril) populations. | 2014 | 25491595 |
| induced resistance to helicoverpa armigera through exogenous application of jasmonic acid and salicylic acid in groundnut, arachis hypogaea. | induced resistance to helicoverpa armigera through exogenous application of jasmonic acid (ja) and salicylic acid (sa) was studied in groundnut genotypes (icgv 86699, icgv 86031, icg 2271 and icg 1697) with different levels of resistance to insects and the susceptible check jl 24 under greenhouse conditions. activities of oxidative enzymes and the amounts of secondary metabolites and proteins were quantified at 6 days after ja and sa application/insect infestation. data were also recorded on pla ... | 2015 | 25488591 |
| enzymatic hydrolysis: a method in alleviating legume allergenicity. | legumes are involved in ige mediated food allergy in many countries. avoidance of allergenic food is the only way to avoid symptomatic reaction. the present study investigated the effect of enzymatic hydrolysis on the allergenicity of three legumes - kidney bean (phaseolus vulgaris), black gram (vigna mungo) and peanut (arachis hypogaea). soluble protein extracts of the study legumes were sequentially treated by alcalase(®) and flavourzyme(®). allergenicity of hydrolysates was then determined by ... | 2015 | 25481434 |
| comparative efficacy of storage bags, storability and damage potential of bruchid beetle. | groundnut during storage is attacked by number of stored grain pests and management of these insect pests particularly bruchid beetle, caryedon serratus (oliver) is of prime importance as they directly damage the pod and kernels. in this regard different storage bags that could be used and duration up to which we can store groundnut has been studied. super grain bag recorded minimum number of eggs laid and less damage and minimum weight loss in pods and kernels in comparison to other storage bag ... | 2014 | 25477679 |
| simultaneous expression of abiotic stress responsive transcription factors, atdreb2a, athb7 and atabf3 improves salinity and drought tolerance in peanut (arachis hypogaea l.). | drought, salinity and extreme temperatures are the most common abiotic stresses, adversely affecting plant growth and productivity. exposure of plants to stress activates stress signalling pathways that induce biochemical and physiological changes essential for stress acclimation. stress tolerance is governed by multiple traits, and importance of a few traits in imparting tolerance has been demonstrated. under drought, traits linked to water mining and water conservation, water use efficiency an ... | 2014 | 25474740 |
| terminal glycotope expression on milk fibronectin differs from plasma fibronectin and changes over lactation. | fibronectin (fn) is a multifunctional glycoprotein appearing in various glycovariants with potential biological activities. using lectins we analyzed the expression of terminal glycotopes on human milk fibronectin over lactation and compared it with that of the mother's plasma. | 2015 | 25464017 |
| kinetic study of solid waste pyrolysis using distributed activation energy model. | the pyrolysis characteristics of municipal solid waste, agricultural residues such as ground nut shell, cotton husk and their blends are investigated using non-isothermal thermogravimetric analysis (tga) with in a temperature range of 30-900 °c at different heating rates of 10 °c, 30 °c and 50 °c/min in inert atmosphere. from the thermograms obtained from tga, it is observed that the maximum rate of degradation occurred in the second stage of the pyrolysis process for all the solid wastes. the d ... | 2015 | 25455087 |
| multilayer graphene-gold nanocomposite modified stem-loop dna biosensor for peanut allergen-ara h1 detection. | in this study, we developed an electrochemically-amplified, stem-loop dna biosensor to detect the peanut allergen ara h1. specifically, we electrodeposited a multilayer graphene-gold nanocomposite onto a glassy carbon electrode and then immobilised a thiolated hairpin dna-biotin probe onto the modified electrode surface. the multilayer graphene-gold composite has good dispersion ability, and can amplify the electrochemical signal due to its high electron-transfer efficiency. the probe was switch ... | 2015 | 25442562 |
| interrelationships between bacillus sp. chep5 and bradyrhizobium sp. semia6144 in the induced systemic resistance against sclerotium rolfsii and symbiosis on peanut plants. | plant-growth-promoting bacteria are often used to enhance crop yield and for biological control of phytopathogens. bacillus sp. chep5 is a biocontrol agent that induces systemic resistance (isr) in arachis hypogaea l. (peanut) against sclerotium rolfsii, the causal agent of root and stem wilt. in this work, the effect of the co-inoculation of bacillus sp. chep5 and the peanut nodulating strain bradyrhizobium sp. semia 6144 was studied on induction of both systemic resistance and nodulation proce ... | 2014 | 25431416 |
| role of reactive oxygen species generation and nod factors during the early symbiotic interaction between bradyrhizobia and peanut, a legume infected by crack entry. | we evaluated whether reactive oxygen species (ros) production and the plant antioxidant system are involved in the symbiotic interaction between bradyrhizobia and legumes infected by crack entry, without intracellular infection threads (it) formation, such as arachis hypogaea l. (peanut). the role of bradyrhizobial nod factors (nf) in modulating the plants' oxidative burst was also analysed. | 2015 | 25413288 |
| ara h 2 and ara h 6 sensitization predicts peanut allergy in mediterranean pediatric patients. | peanut allergy (pa) management was improved by the introduction of molecular allergology, but guidelines for mediterranean patients are lacking. we aimed at evaluating peanut component-resolved diagnosis as a diagnostic and prognostic tool in children from southern france. | 2014 | 25376086 |
| antioxidant and antimicrobial properties of water soluble polysaccharide from arachis hypogaea seeds. | the water soluble crude polysaccharide (ahp) was obtained from the aqueous extracts of the arachis hypogaea seeds through hot water extraction followed by ethanol precipitation. antioxidant activities and inhibitory activities against the bacteria of ahp were investigated. ahp at 2 mg/ml was found to inhibit the formation of superoxide anion (55.33 %) and hydroxyl radicals (30.85 %), to scavenge the dpph radical (57.43 %) and to chelate iron ion (27.83 %) in in vitro systems. ahp also exhibited ... | 2014 | 25328235 |
| kazusa marker database: a database for genomics, genetics, and molecular breeding in plants. | in order to provide useful genomic information for agronomical plants, we have established a database, the kazusa marker database (http://marker.kazusa.or.jp). this database includes information on dna markers, e.g., ssr and snp markers, genetic linkage maps, and physical maps, that were developed at the kazusa dna research institute. keyword searches for the markers, sequence data used for marker development, and experimental conditions are also available through this database. currently, 10 pl ... | 2014 | 25320561 |
| an efficient method of agrobacterium-mediated genetic transformation and regeneration in local indian cultivar of groundnut (arachis hypogaea) using grafting. | groundnut (arachis hypogaea l.) is an industrial crop used as a source of edible oil and nutrients. in this study, an efficient method of regeneration and agrobacterium-mediated genetic transformation is reported for a local cultivar gg-20 using de-embryonated cotyledon explant. a high regeneration 52.69 ± 2.32 % was achieved by this method with 66.6 μm 6-benzylaminopurine (bap), while the highest number of shoot buds per explant, 17.67 ± 3.51, was found with 20 μm bap and 10 μm 2,4-dichlorophen ... | 2015 | 25308617 |
| elemental composition of edible nuts: fast optimization and validation procedure of an icp-oes method. | an inductively coupled plasma-optical emission spectrometry method for the speedy simultaneous detection of 19 elements in edible nuts (walnuts: juglans nigra; almonds: prunus dulcis; hazelnuts: corylus avellana; brazil nuts: bertholletia excelsa; cashews: anacardium occidentalle; pistachios: pistacia vera; and peanuts: arachis hypogaea) available on the serbian markets, was optimized and validated through the selection of instrumental parameters and analytical lines free from spectral interfere ... | 2015 | 25298065 |
| histone deacetylation modification participates in the repression of peanut (arachis hypogaea l.) seed storage protein gene ara h 2.02 during germination. | genes encoding seed storage proteins (ssps) are specifically and highly expressed during seed maturation. in arabidopsis, chromatin-based mechanisms involved in the repression of ssps during germination have been proposed. however, epigenetic regulation involved in repressing ssps in vegetative tissues of peanut is not well understood. histone deacetylase (hdac) is a chromatin-remodelling factor that contributes to transcriptional repression in eukaryotes. to address whether histone deacetylatio ... | 2015 | 25262939 |
| effect of dilution rate on feline urethral sperm motility, viability, and dna integrity. | this study was designed to investigate if the characteristics of feline urethral sperm can be affected by high dilution in an artificial medium. the semen collected by urethral catheterization from eight male cats was evaluated for sperm concentration and motility and subsequently diluted with a tris-based extender to the concentration of spermatozoa 10 × 10(6)/ml, 5 × 10(6)/ml, and 1 × 10(6)/ml. immediately after the extension samples were assessed for motility, cell viability using sybr-14 and ... | 2014 | 25262548 |
| controlled release study on microencapsulated mixture of fipronil and chlorpyrifos for the management of white grubs (holotrichia parallela) in peanuts (arachis hypogaea l.). | this study was conducted to determine the release dynamics of a microencapsulated mixture of fipronil and chlorpyrifos in peanut fields and its efficacy against white grubs. the results indicated that microencapsulation significantly stabilized this mixture against degradation in the environment so that a single dose of this microencapsulated formulation applied through seed treatment effectively controlled white grubs throughout the entire growing season. during the experimental course, the con ... | 2014 | 25260064 |
| ultrasensitive carbohydrate-peptide spr imaging microarray for diagnosing ige mediated peanut allergy. | severity of peanut allergies is linked to allergen-specific immunoglobulin e (ige) antibodies in blood, but diagnostics from assays using glycoprotein allergen mixtures may be inaccurate. measuring iges specific to individual peptide and carbohydrate epitopes of allergenic proteins is promising. we report here the first immunoarray for iges utilizing both peptide and carbohydrate epitopes. a surface plasmon resonance imaging (spri) microarray was equipped with peptide and β-xylosyl glycoside (bx ... | 2014 | 25259443 |
| relationship between biomass, seed components and seed cd concentration in various peanut (arachis hypogaea l.) cultivars grown on cd-contaminated soils. | peanuts (arachis hypogaea l.) exhibit high genotypic variations in seed cd accumulation, but the mechanism remains unclear. this study aimed to reveal the main factors that determine cd concentration in peanut seeds. the biomasses and cd accumulation in plant tissues as well as the cd distribution in the seeds of 15 peanut cultivars were analyzed in a pot experiment at 4mgkg(-1) cd (treatment) and 0mgkg(-1) cd (control). peanuts exhibited large variations among cultivars in terms of cd accumulat ... | 2014 | 25244685 |
| modulatory effects of alpha-linolenic acid on generation of reactive oxygen species in elaidic acid enriched peritoneal macrophages in rats. | fatty acids are known to influence the ability of macrophages to generate reactive oxygen species (ros). however the effect of elaidic acid (ea, 18:1 trans fatty acid) on ros generation is not well studied. rat peritoneal macrophages were enriched with elaidic acid by incubating the cells with 80 1m ea. the macrophages containing ea generated higher amounts of superoxide anion (o2*-), hydrogen peroxide (h2o2) and nitric oxide (no) by 54, 123 and 237%, respectively as compared to control cells wh ... | 2014 | 25241585 |
| mining tissue-specific contigs from peanut (arachis hypogaea l.) for promoter cloning by deep transcriptome sequencing. | peanut (arachis hypogaea l.), one of the most important oil legumes in the world, is heavily damaged by white grubs. tissue-specific promoters are needed to incorporate insect resistance genes into peanut by genetic transformation to control the subterranean pests. transcriptome sequencing is the most effective way to analyze differential gene expression in this non-model species and contribute to promoter cloning. the transcriptomes of the roots, seeds and leaves of peanut were sequenced using ... | 2014 | 25231965 |
| determining the major bt refuge crops for cotton bollworm in north china. | evaluation of the effectiveness of refuge strategies involved in cotton bollworm bt resistance management would be aided by technologies that allow monitoring and quantification of key factors that affect the process under field conditions. we hypothesized that characterization of stable carbon and nitrogen isotopes in adult bollworm, helicoverpa armigera (hübner) (lepidoptera: noctuidae) moths may aid in determining the larval host that they developed upon. we found moths reared from larvae fed ... | 2015 | 25196072 |
| synthesis, micellization and lectin binding of new glycosurfactants. | here we report the preparation and physico-chemical characterization of carbohydrate-decorated micelles and their interaction with lectins. a library of biosourced amphiphiles was prepared by copper-catalyzed azide-alkyne cycloaddition (cuaac) between alkynyl sugars (lactose, n-acetyl-d-glucosamine) and azido-functionalized poly(ethylene glycol) esters (n3-peg900-decanoate (c10) and -dodecanoate (c12)). in water, these glycoconjugates self-assemble into micelles of homogeneous nanometric size (1 ... | 2014 | 25193382 |
| comparative and evolutionary analysis of major peanut allergen gene families. | peanut (arachis hypogaea l.) causes one of the most serious food allergies. peanut seed proteins, arah1, arah2, and arah3, are considered to be among the most important peanut allergens. to gain insights into genome organization and evolution of allergen-encoding genes, approximately 617 kb from the genome of cultivated peanut and 215 kb from a wild relative were sequenced including three arah1, one arah2, eight arah3, and two arah6 gene family members. to assign polarity to differences between ... | 2014 | 25193311 |
| annotation of stress responsive candidate genes in peanut ests. | peanut (arachis hypogaea l.) is an internationally important crop for human consumption as a good source of protein and vegetable oil. peanut is widely cultivated around the world in tropical, sub-tropical and warm temperate climate. because of its huge genome size (2.8 gb) and unsequenced genome, studies on genomics and genetic modification of peanut are less as compared to other model crops. as peanut can be cultivated in arid and semi-arid regions, and its growth is drastically affected by va ... | 2014 | 25183351 |
| high efficiency transformation of banana [musa acuminata l. cv. matti (aa)] for enhanced tolerance to salt and drought stress through overexpression of a peanut salinity-induced pathogenesis-related class 10 protein. | bananas and plantains (musa spp. l.) are important subsistence crops and premium export commodity in several countries, and susceptible to a wide range of environmental and biotic stress conditions. here, we report efficient, rapid, and reproducible agrobacterium-mediated transformation and regeneration of an indian niche cultivar of banana [m. acuminata cv. matti (aa)]. apical meristem-derived highly proliferative multiple shoot clump (msc) explants were transformed with the agrobacterium strai ... | 2015 | 25173686 |
| quality traits of indian peanut cultivars and their utility as nutritional and functional food. | peanut (arachis hypogaea l.) is considered as a highly nutritious foodstuff. of late, the importance of peanut as a functional food has been growing. kernels of forty-one indian peanut cultivars were analyzed for their oil, fatty acid profiles, sucrose, raffinose family oligosaccharides (rfos); phenolics, and free amino acids contents along with antioxidant capacity. the range and the mean value (given in parenthesis) for each of the traits analysed were, oil: 44.1-53.8% (50.1%), o/l ratio: 0.9- ... | 2015 | 25148966 |
| [effects of root cutting under different seedling conditions on root system distribution and senescence character of peanut]. | the effects of root cutting on root system distribution and senescence character of peanut (arachis hypogaea) under different seedling conditions were investigated by using the box culture method. the results showed that, with three types of peanut seedlings, including overgrowing, strong and week seedlings, root cutting all first restricted and then promoted the root system growth, especially promoted the root growth to deep soil. this effect was stronger on the overgrowing and strong seedlings ... | 2014 | 25129940 |
| composting of cow dung and crop residues using termite mounds as bulking agent. | the present study reports the suitability of termite mounds as a bulking agent for composting with crop residues and cow dung in pit method. use of 50 kg termite mound with the crop residues (stover of ground nut: 361.65 kg; soybean: 354.59 kg; potato: 357.67 kg and mustard: 373.19 kg) and cow dung (84.90 kg) formed a good quality compost within 70 days of composting having nitrogen, phosphorus and potassium as 20.19, 3.78 and 32.77 g kg(-1) respectively with a bulk density of 0.85 g cm(-3). oth ... | 2014 | 25108475 |
| dynamic succession of soil bacterial community during continuous cropping of peanut (arachis hypogaea l.). | plant health and soil fertility are affected by plant-microbial interactions in soils. peanut is an important oil crop worldwide and shows considerable adaptability, but growth and yield are negatively affected by continuous cropping. in this study, 16s rrna gene clone library analyses were used to study the succession of soil bacterial communities under continuous peanut cultivation. six libraries were constructed for peanut over three continuous cropping cycles and during its seedling and pod- ... | 2014 | 25010658 |
| gatekeeper tyrosine phosphorylation is autoinhibitory for symbiosis receptor kinase. | plant receptor-like kinases (rlks) are distinguished by having a tyrosine in the 'gatekeeper' position. previously we reported symbiosis receptor kinase from arachis hypogaea (ahsymrk) to autophosphorylate on the gatekeeper tyrosine (y670), though this phosphorylation was not necessary for the kinase activity. here we report that recombinant catalytic domain of ahsymrk with a phosphomimic substitution in the gatekeeper position (y670e) is catalytically almost inactive and is conformationally qui ... | 2014 | 24996184 |
| identification of rapidly induced genes in the response of peanut (arachis hypogaea) to water deficit and abscisic acid. | peanut (arachis hypogaea) is an important crop, but droughts often affect peanut production. there is a lack of genomic information available for peanut; therefore, little is known about the molecular basis of its drought stress response. | 2014 | 24970488 |
| ectopic over-expression of peroxisomal ascorbate peroxidase (sbpapx) gene confers salt stress tolerance in transgenic peanut (arachis hypogaea). | peroxisomal ascorbate peroxidase gene (sbpapx) of an extreme halophyte salicornia brachiata imparts abiotic stress endurance and plays a key role in the protection against oxidative stress. the cloned sbpapx gene was transformed to local variety of peanut and about 100 transgenic plants were developed using optimized in vitro regeneration and agrobacterium mediated genetic transformation method. the t0 transgenic plants were confirmed for the gene integration; grown under controlled condition in ... | 2014 | 24954532 |
| mycological and aflatoxin contamination of peanuts sold at markets in kinshasa, democratic republic of congo, and pretoria, south africa. | peanut (arachis hypogaea l.) is an important food crop in sub-saharan africa. in this survey, the mycological and aflatoxin contamination of peanuts collected from kinshasa, democratic republic of congo, and pretoria, south africa, was assessed. twenty peanut samples were purchased randomly at informal markets in the two cities and analysed for mycoflora and aflatoxins (afb1, afb2, afg1 and afg2) using standard methods. the results indicated that 95% of the kinshasa samples and 100% of the preto ... | 2014 | 24914597 |
| identification of erf genes in peanuts and functional analysis of aherf008 and aherf019 in abiotic stress response. | ethylene-responsive factor (erf) play an important role in regulating gene expression in plant development and response to stresses. in peanuts (arachis hypogaea l.), which produce flowers aerially and pods underground, only a few erf genes have been identified so far. this study identifies 63 erf unigenes from 247,313 peanut est sequences available in the ncbi database. the phylogeny, gene structures, and putative conserved motifs in the peanut erf proteins were analysed. comparative analysis r ... | 2014 | 24902799 |
| construction of a snp-based genetic linkage map in cultivated peanut based on large scale marker development using next-generation double-digest restriction-site-associated dna sequencing (ddradseq). | cultivated peanut, or groundnut (arachis hypogaea l.), is an important oilseed crop with an allotetraploid genome (aabb, 2n=4x=40). in recent years, many efforts have been made to construct linkage maps in cultivated peanut, but almost all of these maps were constructed using low-throughput molecular markers, and most show a low density, directly influencing the value of their applications. with advances in next-generation sequencing (ngs) technology, the construction of high-density genetic map ... | 2014 | 24885639 |
| molecular cloning and characterization of npr1 gene from arachis hypogaea. | the npr1 gene was an important regulator for a plant disease resistance. the cdna of npr1 gene was cloned from peanut cultivar ri hua 1 by rapid amplification of cdna ends-polymerase chain reaction (race-pcr). the full length cdna of arachis hypogaea npr1 consisted of 2,078 base pairs with a 1,446 bp open-reading frame encoding 481 amino acids. the predicted npr1 contained the highly conserved functional domains (btb/poz domain from m1 to d116), protein-protein interaction domains (three ankyrin ... | 2014 | 24842264 |
| comparative assessment of herbicide and fungicide runoff risk: a case study for peanut production in the southern atlantic coastal plain (usa). | peanut (arachis hypogaea) is produced intensively in the southern atlantic coastal plain of the eastern usa. to effectively protect the region's water quality data are needed which quantify runoff of pesticides used to protect these crops. fungicides are used intensively yet there is little published data which describe their potential for loss in surface runoff. this study compared runoff of a fungicide, tebuconazole (α-[2-(4-chlorophenyl)ethyl]-α-(1,1-dimethylethyl)-1h-1,2,4-triazole-1-ethanol ... | 2014 | 24836324 |
| effect of chicken, fat and vegetable on glycaemia and insulinaemia to a white rice-based meal in healthy adults. | white rice is the main staple for the majority in the world. the effects of protein, fat and vegetables on the glycaemic and insulinaemic responses to a white rice-based meal have not been reported. the aim of this study was to determine the effect of co-ingesting a high-protein food (breast chicken), a fat (ground nut oil), a leafy vegetable or all three on the glycaemic and insulinaemic responses of white rice in healthy adults. | 2014 | 24817596 |
| hemomucin, an o-glycosylated protein on embryos of the wasp macrocentrus cingulum that protects it against encapsulation by hemocytes of the host ostrinia furnacalis. | it is unclear how endoparasites passively evade their host's immune reactions in most parasite-host systems. hemomucin from the parasitoid wasp macrocentrus cingulum (mchem) is a 97-kda transmembrane protein containing 51 potential o-glycosylation sites that can be specifically recognized by arachis hypogaea lectin. mchem mrna is highly expressed in m. cingulum eggs, morulae and secondary embryos, and mchem protein is mainly located on the extraembryonic membrane of embryos. when secondary embry ... | 2014 | 24776378 |
| arachis hypogaea l. stem and leaf extract improves the sleep behavior of pentobarbital-treated rats. | this study was conducted to evaluate the sedative effects of arachis hypogaea l. stem and leaf extract (ahsle) and determine its effect pathways through γ-aminobutyric acid (gaba)-gated channels on male sprague-dawley rats treated with pentobarbital. ahsle was obtained from 98°c water (3 h, extracted twice). ahsle and flumazenil (a gaba type a receptor antagonist) were administered to the rats orally, whereas pentobarbital sodium and muscimol (a gaba type a receptor agonist) were administered in ... | 2014 | 24748980 |
| overexpression of horsegram (macrotyloma uniflorum lam.verdc.) nac transcriptional factor (munac4) in groundnut confers enhanced drought tolerance. | the nac family being the largest plant-specific transcription factors functions in diverse and vital physiological processes during development. nac proteins are known to be crucial in imparting tolerance to plants against abiotic stresses, such as drought and salinity, but the functions of most of them are still elusive. in this study, we report for the first time expression of the munac4, a member of nac transcription factor from horsegram (macrotyloma uniflorum) conferring drought tolerance. ... | 2014 | 24748414 |
| peanut sprouts extract (arachis hypogaea l.) has anti-obesity effects by controlling the protein expressions of pparγ and adiponectin of adipose tissue in rats fed high-fat diet. | this study aims to find out the effects of peanut sprout extracts on weight controls and protein expressions of transcription factors related to adipocyte differentiation and adipocytokine in rats under high-fat diets. | 2014 | 24741399 |
| combining ability of pod yield and related traits of groundnut (arachis hypogaea l.) under salinity stress. | a study was performed using 6 × 6 f1 diallel population without reciprocals to assess the mode of inheritance of pod yield and related traits in groundnut with imposed salinity stress. heterosis was found for pod number and yield. data on general and specific combining ability (gca and sca) indicated additive and nonadditive gene actions. the gca: sca ratios were much less than unity suggesting predominant role of nonadditive gene effects. cultivars "binachinabadam-2" and "dacca-1" and mutant m6 ... | 2014 | 24737982 |
| a comparative study on the decomposition of edible and non-edible oil cakes in the gangetic alluvial soil of west bengal. | an experiment has been conducted under laboratory conditions to investigate the effect of decomposition of two edible oil cakes, viz. mustard cake (brassica juncea l) and groundnut cake (arachis hypogaea l), and two non-edible oil cakes, viz. mahua cake (madhuca indica gmel) and neem cake (azadirachta indica juss), at the rate of 5.0 t ha(-1) on the changes of microbial growth and activities in relation to transformations and availability of some plant nutrients in the gangetic alluvial (typic h ... | 2014 | 24733437 |
| expression of peanut iron regulated transporter 1 in tobacco and rice plants confers improved iron nutrition. | iron (fe) limitation is a widespread agricultural problem in calcareous soils and severely limits crop production. iron regulated transporter 1 (irt1) is a key component for fe uptake from the soil in dicot plants. in this study, the peanut (arachis hypogaea l.) ahirt1 was introduced into tobacco and rice plants using an fe-deficiency-inducible artificial promoter. induced expression of ahirt1 in tobacco plants resulted in accumulation of fe in young leaves under fe deficient conditions. even un ... | 2014 | 24727792 |
| pharmacokinetics study of arteether loaded solid lipid nanoparticles: an improved oral bioavailability in rats. | arteether (art), an artemisinin derivative, is a life saving drug for multiple drug resistant malaria. it has a deliverance effect in falciparum malaria and cerebral malaria. we have prepared solid lipid nanoparticles (sln) by high pressure homogenization (hph) technique. art-loaded sln (art-sln) has been produced reproducibly with homogeneous particle size. art-sln was characterized for their size measured by zetasizer nano-zs, malvern, uk and by high resolution transmission electron microscopy ... | 2014 | 24657144 |
| estimation of resveratrol content in peanut pericarp and its relation to the in vitro inhibitory activity on carbohydrate metabolizing enzymes. | the aim of this work was the estimation of resveratrol content in two successive extracts (etoac and meoh) of peanuts (arachis hypogaea l.) pericarp of egypt, by tlc and hplc methods. results showed the presence of 3.0 and 0.5 microg/ml resveratrol in etoac and meoh extracts respectively. the in vitro carbohydrate hydrolyzing enzyme inhibition activity showed higher percentage of inhibition of alpha-amylase, alpha-glucosidase and beta-galactosidase with etoac (4.32, 5.93 and 13.7%) than with meo ... | 2014 | 24640596 |
| galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: regulatory roles of cell surface glycans. | galectin-1 is known to be one of the extracellular matrix proteins. to elucidate the biological roles of galectin-1 in cell adhesion and invasion of human anaplastic large cell lymphoma, we performed cell adhesion and invasion assays using the anaplastic large cell lymphoma cell line h-alcl, which was previously established in our laboratory. from the cell surface lectin array, treatment with neuraminidase from arthrobacter ureafaciens which cleaves all linkage types of cell surface sialic acid ... | 2014 | 24589677 |
| physicochemical characteristics, functional properties, and nutritional benefits of peanut oil: a review. | the legume arachis hypogaea, commonly known as peanut or groundnut, is a very important food crop throughout the tropics and subtropics. peanut is one of the most widely used legumes due to its nutrition and taste, and it occupies a rank of major oilseed crop in the world. it has been recognized as a functional food due to its role in a health promoting effect. peanut oil contains a well-balanced fatty acid and antioxidant profile that provide protection against harmful substances especially fre ... | 2014 | 24580558 |
| (22)na influx is significantly lower in salt tolerant groundnut (arachis hypogaea) varieties. | distinct varieties differing in salt tolerance were initially identified from two separate green house experiments using two systems; solution as well as soil culture. the first screening involved a diverse group of 27 cultivars. several physiological traits; chlorophyll stability index (csi), salt tolerance index (sti) and ion content were determined to screen the cultivars for differences in salt tolerance using solution culture in the first experiment. a set of six varieties (three tolerant a ... | 2014 | 24554838 |
| neu1 sialidase regulates the sialylation state of cd31 and disrupts cd31-driven capillary-like tube formation in human lung microvascular endothelia. | the highly sialylated vascular endothelial surface undergoes changes in sialylation upon adopting the migratory/angiogenic phenotype. we recently established endothelial cell (ec) expression of neu1 sialidase (cross, a. s., hyun, s. w., miranda-ribera, a., feng, c., liu, a., nguyen, c., zhang, l., luzina, i. g., atamas, s. p., twaddell, w. s., guang, w., lillehoj, e. p., puché, a. c., huang, w., wang, l. x., passaniti, a., and goldblum, s. e. (2012) neu1 and neu3 sialidase activity expressed in ... | 2014 | 24550400 |
| genetic diversity and population structure of the major peanut (arachis hypogaea l.) cultivars grown in china by ssr markers. | one hundred and forty-six highly polymorphic simple sequence repeat (ssr) markers were used to assess the genetic diversity and population structure of 196 peanut (arachis hypogaea l.) cultivars which had been extensively planted in different regions in china. these ssr markers amplified 440 polymorphic bands with an average of 2.99, and the average gene diversity index was 0.11. eighty-six rare alleles with a frequency of less than 1% were identified in these cultivars. the largest fst or genet ... | 2014 | 24520347 |
| ahdmt1, a fe(2+) transporter, is involved in improving iron nutrition and n2 fixation in nodules of peanut intercropped with maize in calcareous soils. | peanut (arachis hypogaea l.) is an important legume providing edible proteins and n2 fixation. however, iron deficiency severely reduces peanut growth in calcareous soils. the maize/peanut intercropping effectively improves iron nutrition and n2 fixation of peanut under pot and field conditions on calcareous soils. however, little was known of how intercropping regulates iron transporters in peanut. we identified ahdmt1 as a fe(2+) transporter which was highly expressed in mature nodules with st ... | 2014 | 24519544 |
| transgenics in groundnut (arachis hypogaea l.) expressing cry1acf gene for resistance to spodoptera litura (f.). | large number of primary transgenic events were generated in groundnut by an agrobacterium mediated, in planta transformation method to assess the efficacy of cry1acf against the spodoptera litura. the amplification of required size fragment of 750 bp with npt ii primers and 901 bp with cry1acf gene primers confirmed the integration of the gene. the expression of the cry gene was ascertained by elisa in t2 generation, and the maximum concentration of cry protein in transgenic plants reached appro ... | 2013 | 24431503 |
| expression analysis of drought stress specific genes in peanut (arachis hypogaea , l.). | improving drought tolerance through gene manipulation has been of importance for modern agriculture, which requires identification and validation of candidate genes. prospecting candidate genes from drought adapted crop species is of immense significance. to identify candidate stress responsive genes from adapted crop, we carried out expression analysis of a few drought responsive ests from arachis hypogaea l. (peanut). the expression patterns of nine ahdr (arachis hypogea drought responsive) cl ... | 2013 | 24431496 |
| interrelationship between viscosity and electrical properties for edible oils. | electrical properties of edible oil depend upon its total polar component, temperature and the frequency of the applied voltage. dielectric constant, dielectric loss tangent and electrical conductivity were measured for cottonseed (gossypium sp.), ground nut (apios americana), mustard (brasicca compestriss) and sun flower (helianthus annuus) oils in the temperature range of 20 to 100°c so as to assess the potential of their applicability for assessing the quality of oils. viscosity of the oils i ... | 2013 | 24425951 |
| strategies to mitigate peanut allergy: production, processing, utilization, and immunotherapy considerations. | peanut (arachis hypogaea l.) is an important crop grown worldwide for food and edible oil. the surge of peanut allergy in the past 25 years has profoundly impacted both affected individuals and the peanut and related food industries. in response, several strategies to mitigate peanut allergy have emerged to reduce/eliminate the allergenicity of peanuts or to better treat peanut-allergic individuals. in this review, we give an overview of peanut allergy, with a focus on peanut proteins, including ... | 2014 | 24387606 |
| complex thiolated mannose/quinone film modified on eqcm/au electrode for recognizing specific carbohydrate-proteins. | a complex thiolated mannose (tm)/quinone functionalised polythiophene (qfpt) thin film was modified on eqcm/au electrode for recognition of specific carbohydrate-proteins. different lectins such as those from sambucus nigra (elder berry), arachis hypogaea (peanut), ulex europaeus (gorse, furze), triticum vulgaris and concanavalin a (cona) was used for probes to evaluate bio-sensing performance of the tm/qfpt film. a specific response was observed for cona from lectins when using the tm/qfpt film ... | 2014 | 24373955 |
| benefits and limitations of molecular diagnostics in peanut allergy: part 14 of the series molecular allergology. | allergic reactions to peanut (arachis hypogaea, ara h) are caused by immunoglobulin e (ige)-mediated sensitizations to various proteins. the stability and relative proportion of these proteins in peanut determine the risk of hazardous reactions. hazardous sensitization to seed storage proteins [s2 albumins (ara h 2, 6 and 7) > other seed storage proteins (ara 1 and 3) > oleosins (ara h 10 and 11)] are distinct from sensitizations to lipid transfer protein (ara h 9) with moderate risk or cross-se ... | 2017 | 26120527 |