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the effect of delactosed whey permeate on phytochemical content of canned tomatoes.the effect of delactosed whey permeate (dwp) treatment on antioxidant and phyto-chemical components of canned irish plum tomatoes were investigated. tomatoes were sterilised for 5 min (f(0)) at 120 °c and stored for 6 months. the dwp treatment retained significantly (p<0.05) higher levels of ascorbic acid and lycopene of tomatoes. the antioxidant activity of dwp treated tomatoes was higher (7%) than the control at the end of storage. the firmness in dwp-treated fruits was around 40% higher than ...201223442681
phylogeography of anastrepha obliqua inferred with mtdna sequencing.anastrepha obliqua (macquart) (diptera: tephritidae), the west indian fruit fly, is a frugivorous pest that occasionally finds its way to commercial growing areas outside its native distribution. it inhabits areas in mexico, central and south america, and the caribbean with occasional infestations having occurred in the southern tier states (california, florida, and texas) of the united states. this fly is associated with many plant species and is a major pest of mango and plum. we examine the g ...201223356081
association between chloroplast and mitochondrial dna sequences in chinese prunus genotypes (prunus persica, prunus domestica, and prunus avium).the nuclear dna is conventionally used to assess the diversity and relatedness among different species, but variations at the dna genome level has also been used to study the relationship among different organisms. in most species, mitochondrial and chloroplast genomes are inherited maternally; therefore it is anticipated that organelle dna remains completely associated. many research studies were conducted simultaneously on organelle genome. the objectives of this study was to analyze the genet ...201525592231
transcriptomic analysis of prunus domestica undergoing hypersensitive response to plum pox virus infection.plum pox virus (ppv) infects prunus trees around the globe, posing serious fruit production problems and causing severe economic losses. one variety of prunus domestica, named 'jojo', develops a hypersensitive response to viral infection. here we compared infected and non-infected samples using next-generation rna sequencing to characterize the genetic complexity of the viral population in infected samples and to identify genes involved in development of the resistance response. analysis of vira ...201424959894
highly efficient transformation protocol for plum (prunus domestica l.).a high-throughput transformation system for plum has been developed using hypocotyl slices excised from zygotic embryos as the source of explants. the hypocotyl slices are infected in an agrobacterium tumefaciens suspension and then cocultivated for 3 days in shoot regeneration ¾ ms basal medium supplemented with 9 μm 2,4-dichlorophenoxyacetic acid. transgenic shoots are regenerated in a medium containing 7.5 μm thidiazuron and elongated in a medium containing 3 μm benzyladenine in the presence ...201222351009
transgenic plums (prunus domestica l.) express the plum pox virus coat protein gene.plum hypocotyl slices were transformed with the coat protein (cp) gene of plum pox virus (ppv-cp) following cocultivation with agrobacterium tumefaciens containing the plasmid pga482gg/ppvcp-33. this binary vector carries the ppv-cp gene construct, as well as the chimeric neomycin phosphotransferase and β-glucuronidase genes. integration and expression of the transferred genes into regenerated plum plants was verified through kan resistance, gus assays, and pcr amplification of the ppv-cp gene. ...199424194220
[identification of pantoea agglomeran xm2 with biocontrol activity against postharvest pear black spot].we isolated the bacterial strain xm2 from prunes (prunus domestica l.) fruit surface. xm2 was identified and tested as an antagonist for postharvest biological control of black spot disease (alternaria alternata) on pear fruits.201425272813
effects of solid-state fermentation with two filamentous fungi on the total phenolic contents, flavonoids, antioxidant activities and lipid fractions of plum fruit (prunus domestica l.) by-products.evolutions of phenolic contents and antioxidant activities during solid-state fermentation (ssf) of plum pomaces (from the juice industry) and brandy distillery wastes with aspergillus niger and rhizopus oligosporus were investigated. the effect of fermentation time on the oil content and major lipid classes in the plum kernels was also studied. results showed that total phenolic (tp) amounts increased by over 30% for ssf with rhizopus oligosporus and by >21% for ssf with a. niger. the total fla ...201627173530
incidence and severity of leaf and fruit diseases of plums in latvia.in the present study six plum orchards in latvia were examined during 2014. one orchard was commercial with integrated pest management (ipm) practices, one was with organic management, two orchards were scientific collections and in two orchards plums were grown as a minor crop, using ipm practices. the shot-hole disease (wilsonomyces carpophilus) and fruit rot were monitored in the field. samples of twigs and leaves were taken for further examination if some other disease symptoms were observed ...201527141740
screening of solvent dependent antibacterial activity of prunus domestica.fruit of prunus domestica was extracted in ethanol. the ethanol extract was further extracted with two solvents ethyl acetate and chloroform. the crude ethanol extract and two fractions (ethyl acetate and chloroform) were screened for their antibacterial activity using the agar well diffusion method .they were tested against nine bacteria; five gram positive bacteria (staphylococcus aureus, streptococcuc intermedius, bacillus cereus, bacillus pumilus) and four gram negative bacteria (eschrichia ...201323455215
a synergistic effect in controlling plum postharvest diseases occurs by applying uv-c light to sodium bicarbonate treated fruit.the effectiveness of ultraviolet-c light (uv-c; 254 nm) alone at 0, 3, 6 and 12 kjm(-2), or combined with 0.0 or 0.5% (w7v) sodium bicarbonate (sbc), to control plum (prunus domestica cv stanley) postharvest decay caused by penicillium expansum l. and botrytis cinerea was investigated. first, fruit was sanitized and in one experiment plums were artificially wound-inoculated 24 h before treatments and afterwards kept at 25 degrees c with 90% rh for 7 days. in the second experiment, treatments wer ...201325151834
regulatory/modulatory effect of prune essence concentrate on intestinal function and blood lipids.prunus domestica linn (rosaceae) has been considered a functional food, owing to its various pharmacological activities, including antioxidant, anti-inflammatory, antidiabetic and anticancer.201728164731
endophytic fungi from plums (prunus domestica) and their antifungal activity against monilinia fructicola.enophytic fungi were isolated from plum (prunus domestica) leaves, identified with its1 and its4 primers, and their antagonistic activity was tested against monilinia fructicola, which causes brown rot, blossom blight, and twig blight of stone fruits, and colletotrichum gloeosporioides, which causes anthracnose on a variety of fruit crops. the production of antifungal compounds was determined in agar-diffusion and volatile inverted-plate tests. a total of 163 fungi were recovered from 30 plum tr ...201223043843
anti-inflammatory and cytoprotective effects of selected pakistani medicinal plants in helicobacter pylori-infected gastric epithelial cells.helicobacter pylori infection is associated with gastritis, peptic ulcer, and gastric cancer. due to its high global prevalence and uprising resistance to available antibiotics, efforts are now directed to identify alternative source to treat and prevent associated disorders. in the present study, effect of selected indigenous medicinal plants of pakistan was evaluated on the secretion of interleukin-8 (il-8) and generation of reactive oxygen species (ros) in a bid to rationalize their medicinal ...201222433535
alternative methods to control postharvest decay caused by penicillium expansum in plums (prunus domestica l.).in the latest years, investigation on postharvest treatments has been increasingly addressed to preserve human health and environment safeguard. several preservative compounds, physical treatments and biological control agents to restrain postharvest pathogens on horticultural products have been widely studied. among them potassium sorbate (ks) has been generally recognized as safe for use in foods and personal care products. it acts as microbial growth inhibitor and fungistatic agent in foods, ...201223885418
plums (prunus domestica l.) are a good source of yeasts producing organic acids of industrial interest from glycerol.the production of organic acids from several yeasts isolated from mature plums on media containing glycerol as carbon source was analysed by hplc-uv. the yeasts isolated were identified by sequencing the 5.8s internal transcribed spacer as pichia fermentans, wickerhamomyces anomalus and candida oleophila. the organic acid profiles of these strains comprise acetic, citric, succinic and malic acids that qualitatively and quantitatively vary between different species as well as among strains from t ...201323561074
sugars and organic acids in plum fruit affected by plum pox virus.plum pox virus (ppv) causes severe economic losses in stone fruit production, but little is known about its effect on plum fruit composition. in this study, the influence of ppv on sugars and organic acids was evaluated in a susceptible plum (prunus domestica l.) cultivar.201727614092
aphid transmission of the ontario isolate of plum pox virus.utilization of timed virus acquisition access probes in studies of plum pox virus (ppv) transmission by aphids demonstrated that endemic species transmitted the virus readily from plum, prunus domestica (l.) batsch; peach, p. persica (l.); or dwarf flowering almond, p. glandulosa thunberg., to peach seedlings. the green peach aphid, myzus persicae (sulzer), was shown to be the most efficient vector. acquisition of virus by green peach aphids from infected peach leaves resulted in 18-28% infected ...201526453705
effect of plum pox virus on chemical composition and fruit quality of plum.the quality of fruit from ppv (plum pox virus)-infected plum (prunus domestica l.) trees was investigated during the last 3 weeks of ripening using healthy tree (t3), tree with short-term infection (t2), and tree with long-term infection (t1). the pomological variables (presence of necrosis on fruit, color of fruit, color of flesh, firmness, soluble solids content, and fruit weight) and composition of nutritive compounds (sugars and organic acids) and bioactive compounds (phenolics: anthocyanins ...201525495040
complete genome sequence of a novel plum pox virus strain w isolate determined by 454 pyrosequencing.the near-complete (99.7 %) genome sequence of a novel russian plum pox virus (ppv) isolate pk, belonging to the strain winona (w), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. this region was amplified using 5'race kit and sequenced by the sanger method. genomic rna released from immunocaptured ppv particles was employed for generation of cdna library using transplex whole transcriptome amplification kit (wta2, sigma-aldrich). the entire ...201323813250
silencing of the host factor eif(iso)4e gene confers plum pox virus resistance in plum.plum pox virus (ppv) causes the most economically-devastating viral disease in prunus species. unfortunately, few natural resistance genes are available for the control of ppv. recessive resistance to some potyviruses is associated with mutations of eukaryotic translation initiation factor 4e (eif4e) or its isoform eif(iso)4e. in this study, we used an rna silencing approach to manipulate the expression of eif4e and eif(iso)4e towards the development of ppv resistance in prunus species. the eif4 ...201323382802
evaluation of the genetic diversity of plum pox virus in a single plum tree.genetic diversity of plum pox virus (ppv) and its distribution within a single perennial woody host (plum, prunus domestica) has been evaluated. a plum tree was triply infected by chip-budding with ppv-m, ppv-d and ppv-rec isolates in 2003 and left to develop untreated under open field conditions. in september 2010 leaf and fruit samples were collected from different parts of the tree canopy. a 745-bp nib-cp fragment of ppv genome, containing the hypervariable region encoding the cp n-terminal e ...201222521553
spatial and temporal assessment of pollen- and seed-mediated gene flow from genetically engineered plum prunus domestica.pollen flow from a 0.46 ha plot of genetically engineered (ge) prunus domestica located in west virginia, usa was evaluated from 2000-2010. sentinel plum trees were planted at distances ranging from 132 to 854 m from the center of the ge orchard. plots of mixed plum varieties and seedlings were located at 384, 484 and 998 m from the ge plot. bee hives (apis mellifera) were dispersed between the ge plum plot and the pollen flow monitoring sites. pollen-mediated gene flow from out of the ge plum p ...201324098374
categorization of cathartic (purgative) medicines mentioned in tpm resources according to their specific function.according to traditional persian medicine (tpm) resources, the human digestive system includes four steps. in the first step, gastric digestion, the ingested food pours into the stomach and changes into the leachate called chylous due to the heat produced in the stomach. in the second step, hepatic digestion, the chylous enters in the liver through mesenteric vessels and transforms into the quadruple humors, sanguine, phlegm, bile and black bile due to the liver heat. in the case of humor predom ...201627840491
categorization of cathartic (purgative) medicines mentioned in tpm resources according to their specific function.according to traditional persian medicine (tpm) resources, the human digestive system includes four steps. in the first step, gastric digestion, the ingested food pours into the stomach and changes into the leachate called chylous due to the heat produced in the stomach. in the second step, hepatic digestion, the chylous enters in the liver through mesenteric vessels and transforms into the quadruple humors, sanguine, phlegm, bile and black bile due to the liver heat. in the case of humor predom ...201627516658
activity, polypeptide and gene identification of thylakoid ndh complex in trees: potential physiological relevance of fluorescence assays.three evergreen (laurus nobilis, viburnum tinus and thuja plicata) and two autumnal abscission deciduous trees (cydonia oblonga and prunus domestica) have been investigated for the presence (zymogram and immunodetection) and functionality (post-illumination chlorophyll fluorescence) of the thylakoid ndh complex. the presence of encoding ndh genes has also been investigated in t. plicata. western assays allowed tentative identification of zymogram nadh dehydrogenase bands corresponding to the ndh ...201222324908
effect of increasing levels of seven tree species extracts added to a high concentrate diet on in vitro rumen gas output.this study was conducted to investigate the effects of increasing levels of extracts of byrsonima crassifolia, celtis pallida, enterolobium cyclocarpum, fraxinus excelsior, ficus trigonata, phoradendrom brevifolium and prunus domestica on in vitro gas production (gp) and ruminal fermentation of a high concentrate diet. plant extracts were prepared at 1 g dry matter (dm)/8 ml of solvent mixture (methanol : ethanol : water, 1:1:8) and added at levels of 0, 0.6, 1.2 and 1.8 ml/g dm of a high concen ...201424796241
eriophyoid mites (acari: trombidiformes: eriophyoidea) of rosales trees in iran: two new species and three new records.this paper describes two new species of eriophyoidea associated with trees belonging to the order rosales in the south-western portion of east azerbaijan province, iran, collected during a survey in 2011: aceria lobolinguae n. sp. on elaeagnus angustifolia l. (elaeagnaceae) and rhinophytoptus nemalobos n. sp. on prunus domestica l. (rosaceae). additionally, phyllocoptes abaenus keifer on prunus armeniaca l. (rosaceae), aculus fockeui (nalepa & trouessart) on prunus amygdalus stokes and malus dom ...201425283393
neophyllobius lorestanicus sp. nov. and n. ostovani sp.nov. (acari: camerobiidae) from iran.two new species of the genus neophyllobius berlese, 1886 are described: neophyllobius lorestanicus sp. nov. collected from soil under prunus domestica l. (rosaceae) in markazi province and neophyllobius ostovani sp. nov. from soil and rotten leaves of oak trees in fars province, iran. a key to all known iranian and turkish species of neophyllobius is provided.201424870646
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