| cloning and expression analysis of hsp70 gene from mangrove plant kandelia obovata under cold stress. | heat shock protein 70 (hsp70), the primary member of the hsps that play various stress-protective roles in plants. in this study, a hsp70 gene of kandelia obovata (kohsp70) was cloned by rapid amplification of cdna ends (race). the full-length of kohsp70 was 2255 bp, consisting of a 5'-terminal untranslated region (utr) of 118 bp, a 3'-terminal utr of 178 bp, and an open reading frame (orf) of 1959 bp. the orf (kohsp70) was predicted to encode a polypeptide of 652 amino acids with a theoretical ... | 2015 | 25980488 |
| molecular cloning and expression analysis of ghlof genes in upland cotton (gossypium hirsutum l.). | shoot branching, i.e., the timing and position of shoot growth, determines to a large extend the pattern of plant architecture, and is the result of the integration of a plant's genetic background and environmental cues. many genes that are involved in the formation and outgrowth of axillary buds have been cloned, but the exact mechanism is still unclear. branching pattern is an important agronomic trait in many crops, including cotton. in the present study, we cloned four genes from cotton, and ... | 2015 | 25966231 |
| development of gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the g. anomalum and g. hirsutum genomes. | we reported the first development of gossypium anomalum -derived microsatellite markers and identification of recombination between sexually incompatible species by a synthesized hexaploid on genome level. to continue to develop improved cotton varieties, it is essential to transfer desired characters from diploid wild cotton species such as gossypium anomalum to cultivated allotetraploid cotton species. however, interspecific reproductive barriers limit gene transfer between species. in a previ ... | 2015 | 25957115 |
| genetic mapping and comparative expression analysis of transcription factors in cotton. | transcription factors (tfs) play an important role in the regulation of plant growth and development. the study of the structure and function of tfs represents a research frontier in plant molecular biology. the findings of these studies will provide significant information regarding genetic improvement traits in crops. currently, a large number of tfs have been cloned, and their function has been verified. however, relatively few studies that genetically map tfs in cotton are available. to gene ... | 2015 | 25946129 |
| development of a 63k snp array for cotton and high-density mapping of intraspecific and interspecific populations of gossypium spp. | high-throughput genotyping arrays provide a standardized resource for plant breeding communities that are useful for a breadth of applications including high-density genetic mapping, genome-wide association studies (gwas), genomic selection (gs), complex trait dissection, and studying patterns of genomic diversity among cultivars and wild accessions. we have developed the cottonsnp63k, an illumina infinium array containing assays for 45,104 putative intraspecific single nucleotide polymorphism ( ... | 2015 | 25908569 |
| molecular mapping of restriction-site associated dna markers in allotetraploid upland cotton. | upland cotton (gossypium hirsutum l., 2n = 52, aadd) is an allotetraploid, therefore the discovery of single nucleotide polymorphism (snp) markers is difficult. the recent emergence of genome complexity reduction technologies based on the next-generation sequencing (ngs) platform has greatly expedited snp discovery in crops with highly repetitive and complex genomes. here we applied restriction-site associated dna (rad) sequencing technology for de novo snp discovery in allotetraploid cotton. we ... | 2015 | 25894395 |
| sequencing of allotetraploid cotton (gossypium hirsutum l. acc. tm-1) provides a resource for fiber improvement. | upland cotton is a model for polyploid crop domestication and transgenic improvement. here we sequenced the allotetraploid gossypium hirsutum l. acc. tm-1 genome by integrating whole-genome shotgun reads, bacterial artificial chromosome (bac)-end sequences and genotype-by-sequencing genetic maps. we assembled and annotated 32,032 a-subgenome genes and 34,402 d-subgenome genes. structural rearrangements, gene loss, disrupted genes and sequence divergence were more common in the a subgenome than i ... | 2015 | 25893781 |
| genome sequence of cultivated upland cotton (gossypium hirsutum tm-1) provides insights into genome evolution. | gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (atdt) genome. here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold bac-to-bac sequences and a high-resolution genetic map. in our assembly 88.5% of the 2,173-mb scaffolds, which cover 89.6%∼96.7% of the atdt genome, are anchored and oriented to 26 pseudochromosomes. comparison of this g. hirsutum atdt genome with the already sequenced diploid gossypium arboreum (aa) and gos ... | 2015 | 25893780 |
| arabidopsis edt1/hdg11 improves drought and salt tolerance in cotton and poplar and increases cotton yield in the field. | drought and salinity are two major environmental factors limiting crop production worldwide. improvement of drought and salt tolerance of crops with transgenic approach is an effective strategy to meet the demand of the ever-growing world population. arabidopsis enhanced drought tolerance1/homeodomain glabrous11 (atedt1/hdg11), a homeodomain-start transcription factor, has been demonstrated to significantly improve drought tolerance in arabidopsis, tobacco, tall fescue and rice. here we report t ... | 2016 | 25879154 |
| bac-end sequence-based snp mining in allotetraploid cotton (gossypium) utilizing resequencing data, phylogenetic inferences, and perspectives for genetic mapping. | a bacterial artificial chromosome library and bac-end sequences for cultivated cotton (gossypium hirsutum l.) have recently been developed. this report presents genome-wide single nucleotide polymorphism (snp) mining utilizing resequencing data with bac-end sequences as a reference by alignment of 12 g. hirsutum l. lines, one g. barbadense l. line, and one g. longicalyx hutch and lee line. a total of 132,262 intraspecific snps have been developed for g. hirsutum, whereas 223,138 and 470,631 inte ... | 2015 | 25858960 |
| cottonseed protein, oil, and mineral status in near-isogenic gossypium hirsutum cotton lines expressing fuzzy/linted and fuzzless/linted seed phenotypes under field conditions. | cotton is an important crop in the world and is a major source of oil for human consumption and cotton meal for livestock. cottonseed nutrition (seed composition: protein, oil, and minerals) determines the quality of seeds. therefore, maintaining optimum levels of cottonseed nutrition is critical. physiological and genetic mechanisms controlling the levels of these constituents in cottonseed are still largely unknown. our previous research conducted under greenhouse conditions showed that seed a ... | 2015 | 25852704 |
| genome-wide analysis of the family 1 glycosyltransferases in cotton. | family 1 gt, designated as ugt, is the largest and most functionally important multigene family in the plant kingdom. in this study, we carried out a genome-wide identification, analysis, and comparison of 142, 146, and 196 putative ugts from gossypium raimondii, gossypium arboreum, and gossypium hirsutum, respectively. all members present the 44 amino-acid conserved consensus sequence termed the plant secondary product glycosyltransferase motif. according to the phylogenetic relationship among ... | 2015 | 25851236 |
| global analysis of the gossypium hirsutum l. transcriptome during leaf senescence by rna-seq. | leaf senescence is an important developmental programmed degeneration process that dramatically affects crop quality and yield. the regulation of senescence is highly complex. although senescence regulatory genes have been well characterized in model species such as arabidopsis and rice, there is little information on the control of this process in cotton. here, the senescence process in cotton (gossypium hirsutum l.) leaves was investigated over a time course including young leaf, mature leaf a ... | 2015 | 25849479 |
| rna-seq analysis of short fiber mutants ligon-lintless-1 (li 1 ) and - 2 (li 2 ) revealed important role of aquaporins in cotton (gossypium hirsutum l.) fiber elongation. | cotton fiber length is a key determinant of fiber quality for the textile industry. understanding the molecular basis of fiber elongation would provide a means for improvement of fiber length. ligon lintless-1 (li 1 ) and ligon lintless-2 (li 2 ) are monogenic and dominant mutations, that result in an extreme reduction in the length of lint fiber to approximately 6 mm on mature seeds. in a near-isogenic state with wild type (wt) cotton these two short fiber mutants provide an excellent model sys ... | 2015 | 25848981 |
| genetic diversity, population structure and marker trait associations for seed quality traits in cotton (gossypium hirsutum). | cottonseed contains 16% seed oil and 23% seed protein by weight. high levels of palmitic acid provides a degree of stability to the oil, while the presence of bound gossypol in proteins considerably changes their properties, including their biological value. this study uses genetic principles to identify genomic regions associated with seed oil, protein and fibre content in upland cotton cultivars. cotton association mapping panel representing the us germplasm were genotyped using amplified frag ... | 2015 | 25846880 |
| down-regulation of the cotton endo-1,4-β-glucanase gene kor1 disrupts endosperm cellularization, delays embryo development, and reduces early seedling vigour. | towards the aim of examining the potential function of korrigan (kor), a highly conserved membrane-bound endoglucanase, in reproductive development, here transgenic evidence is provided that a cotton (gossypium hirsutum) endoglucanase, ghkor1, plays significant roles in endosperm and embryo development. rna interference (rnai)- and co-suppression-mediated down-regulation of ghkor1 resulted in smaller filial tissue and reduced seed weight, which were characterized by disrupted endosperm cellulari ... | 2015 | 25805716 |
| distribution and evolution of cotton fiber development genes in the fibreless gossypium raimondii genome. | cotton fiber represents the largest single cell in plants and they serve as models to study cell development. this study investigated the distribution and evolution of fiber unigenes anchored to recombination hotspots between tetraploid cotton (gossypium hirsutum) at and dt subgenomes, and within a parental diploid cotton (gossypium raimondii) d genome. comparative analysis of at vs d and dt vs d showed that 1) the d genome provides many fiber genes after its merger with another parental diploid ... | 2015 | 25796538 |
| construction of a high-density genetic map and lint percentage and cottonseed nutrient trait qtl identification in upland cotton (gossypium hirsutum l.). | upland cotton plays a critical role not only in the textile industry, but also in the production of important secondary metabolites, such as oil and proteins. construction of a high-density linkage map and identifying yield and seed trait quantitative trail loci (qtl) are prerequisites for molecular marker-assisted selective breeding projects. here, we update a high-density upland cotton genetic map from recombinant inbred lines. a total of 25,313 ssr primer pairs were screened for polymorphism ... | 2015 | 25796191 |
| rnai construct of a cytochrome p450 gene cyp82d109 blocks an early step in the biosynthesis of hemigossypolone and gossypol in transgenic cotton plants. | naturally occurring terpenoid aldehydes from cotton, such as hemigossypol, gossypol, hemigossypolone, and the heliocides, are important components of disease and herbivory resistance in cotton. these terpenoids are predominantly found in the glands. differential screening identified a cytochrome p450 cdna clone (cyp82d109) from a gossypium hirsutum cultivar that hybridized to mrna from glanded cotton but not glandless cotton. both the d genome cotton gossypium raimondii and a genome cotton gossy ... | 2015 | 25794893 |
| complete genome sequences of two new virus isolates associated with cotton blue disease resistance breaking in brazil. | since 2006, brazilian cotton (gossypium hirsutum) crops planted with cultivars that are resistant to cotton blue disease have developed a new disease termed "atypical" cotton blue disease or atypical vein mosaic disease. here, we describe the complete genomes of two virus isolates associated with this disease. the new virus isolates, called clrdv-acr3 and clrdv-ima2, were found to have a high degree of nucleotide and amino acid sequence similarity to previously described isolates of cotton leafr ... | 2015 | 25772571 |
| cotton qtldb: a cotton qtl database for qtl analysis, visualization, and comparison between gossypium hirsutum and g. hirsutum × g. barbadense populations. | a specialized database currently containing more than 2200 qtl is established, which allows graphic presentation, visualization and submission of qtl. in cotton quantitative trait loci (qtl), studies are focused on intraspecific gossypium hirsutum and interspecific g. hirsutum × g. barbadense populations. these two populations are commercially important for the textile industry and are evaluated for fiber quality, yield, seed quality, resistance, physiological, and morphological trait qtl. with ... | 2015 | 25758743 |
| construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (gossypium hirsutum l.). | the first high-density linkage map was constructed to identify quantitative trait loci (qtls) for somatic embryogenesis (se) in cotton ( gossypium hirsutum l.) using leaf petioles as explants. cotton transformation is highly limited by only a few regenerable genotypes and the lack of understanding of the genetic and molecular basis of somatic embryogenesis (se) in cotton (gossypium hirsutum l.). to construct a more saturated linkage map and further identify quantitative trait loci (qtls) for se ... | 2015 | 25758337 |
| gene-expression novelty in allopolyploid cotton: a proteomic perspective. | allopolyploidization is accompanied by changes in gene expression that are thought to contribute to phenotypic diversification. here we describe global changes in the single-celled cotton fiber proteome of two natural allopolyploid species (gossypium hirsutum and g. barbadense) and living models of their diploid parents using two different proteomic approaches. in total, 1323 two-dimensional gel electrophoresis spots and 1652 identified proteins by isobaric tags for relative and absolute quantit ... | 2015 | 25735302 |
| targeted lipidomics studies reveal that linolenic acid promotes cotton fiber elongation by activating phosphatidylinositol and phosphatidylinositol monophosphate biosynthesis. | the membrane lipids from fast-elongating wild-type cotton (gossypium hirsutum) fibers at 10 days post-anthesis, wild-type ovules with fiber cells removed, and ovules from the fuzzless-lintless mutant harvested at the same age, were extracted, separated, and quantified. fiber cells contained significantly higher amounts of phosphatidylinositol (pi) than both ovule samples with pi 34:3 being the most predominant species. the genes encoding fatty acid desaturases (δ(15)ghfad), pi synthase (pis) and ... | 2015 | 25731673 |
| overexpression of cotton rav1 gene in arabidopsis confers transgenic plants high salinity and drought sensitivity. | rav (related to abi3/vp1) protein containing an ap2 domain in the n-terminal region and a b3 domain in the c-terminal region, which belongs to ap2 transcription factor family, is unique in higher plants. in this study, a gene (ghrav1) encoding a rav protein of 357 amino acids was identified in cotton (gossypium hirsutum). transient expression analysis of the egfp:ghrav1 fusion genes in tobacco (nicotiana tabacum) epidermal cells revealed that ghrav1 protein was localized in the cell nucleus. qua ... | 2015 | 25710493 |
| comparative proteomic and biochemical analyses reveal different molecular events occurring in the process of fiber initiation between wild-type allotetraploid cotton and its fuzzless-lintless mutant. | to explore lint fiber initiation-related proteins in allotetraploid cotton (gossypium hirsutum l.), a comparative proteomic analysis was performed between wild-type cotton (xu-142) and its fuzzless-lintless mutant (xu-142-fl) at five developmental time points for lint fiber initiation from -3 to +3 days post-anthesis (dpa). using two-dimensional gel electrophoresis (2-de) combined with mass spectrometry (ms) analyses, 91 differentially accumulated protein (dap) species that are related to fiber ... | 2015 | 25700002 |
| anther response to high-temperature stress during development and pollen thermotolerance heterosis as revealed by pollen tube growth and in vitro pollen vigor analysis in upland cotton. | pollen tube growth in styles was strongly inhibited by temperature above 35 °c, and the yield of cotton decreased because of the adverse effect of high temperatures during square development. high-temperature stress during flowering influences the square development of upland cotton (gossypium hirsutum l.) and cotton yield. although it is well known that square development is sensitive to high temperature, high-temperature sensitive stages of square development and the effects of high temperatur ... | 2015 | 25672505 |
| enrichment of an intraspecific genetic map of upland cotton by developing markers using parental rad sequencing. | rad sequencing was performed using dh962 and jimian5 as upland cotton mapping parents. sequencing data for dh962 and jimian5 were assembled into the genome sequences of ≈55.27 and ≈57.06 mb, respectively. analysing genome sequences of the two parents, 1,323 ssr, 3,838 insertion/deletion (indel), and 9,366 single-nucleotide polymorphism (snp) primer pairs were developed. all of the ssrs, 121 indels, 441 snps, and other 6,747 primer pairs were screened in the two parents, and a total of 535 new po ... | 2015 | 25656006 |
| measurements of transpiration isotopologues and leaf water to assess enrichment models in cotton. | the two-pool and péclet effect models represent two theories describing mechanistic controls underlying leaf water oxygen isotope composition at the whole-leaf level (δ(18) ol ). to test these models, we used a laser spectrometer coupled to a gas-exchange cuvette to make online measurements of δ(18) o of transpiration (δ(18) otrans ) and transpiration rate (e) in 61 cotton (gossypium hirsutum) leaves. δ(18) otrans measurements permitted direct calculation of δ(18) o at the sites of evaporation ( ... | 2015 | 25643590 |
| comparative analysis of resistance gene analogues encoding nbs-lrr domains in cotton. | plant production is severely affected by biotic and abiotic stresses r-genes exhibit resistance against a range of diseases and pathogens in plants. the nucleotide binding site and leucine rich repeat (nbs-lrr) class of r-genes is the most comprehensively studied in terms of sequence evolution and genome distribution. the differential response for resistance against biotic and abiotic stress has been observed in cultivated and wild relatives of the genus gossypium. | 2016 | 25640313 |
| microrna evolution and expression analysis in polyploidized cotton genome. | cotton (gossypium hirsutum l.), the most important fibre plant in the world, is a tetraploid species, originating from the reunion of two ancestral cotton species ~1-2 million years ago. it has been reported that a great number of genes were quickly erased or preferentially remained after whole-genome duplication, ultimately leading to morphogenesis evolution. however, micrornas (mirnas), a new class of gene regulators, have not been well studied in polyploidization. here, we systematically inve ... | 2015 | 25561162 |
| proteomic analysis of anthers from wild-type and photosensitive genetic male sterile mutant cotton (gossypium hirsutum l.). | male sterility is a common phenomenon in flowering plant species, and it has been successfully developed in several crops by taking advantage of heterosis. using space mutation breeding of upland cotton, a novel photosensitive genetic male sterile (pgms) mutant was isolated. to take advantage of the pgms lines in cotton hybrid breeding, it is of great importance to study the molecular mechanisms of its male sterility. | 2014 | 25547499 |
| a r2r3-myb transcription factor that is specifically expressed in cotton (gossypium hirsutum) fibers affects secondary cell wall biosynthesis and deposition in transgenic arabidopsis. | secondary cell wall (scw) is an important industrial raw material for pulping, papermaking, construction, lumbering, textiles and potentially for biofuel production. the process of scw thickening of cotton fibers lays down the cellulose that will constitute the bulk (up to 96%) of the fiber at maturity. in this study, a gene encoding a myb-domain protein was identified in cotton (gossypium hirsutum) and designated as ghmybl1. quantitative real-time polymerase chain reaction (rt-pcr) analysis rev ... | 2015 | 25534543 |
| silicon (si) alleviates cotton (gossypium hirsutum l.) from zinc (zn) toxicity stress by limiting zn uptake and oxidative damage. | silicon (si) is as an important fertilizer element, which has been found effective in enhancing plant tolerance to variety of biotic and a-biotic stresses. this study investigates the si potential to alleviate zinc (zn) toxicity stress in cotton (gossypium hirsutum l.). cotton plants were grown in hydroponics and exposed to different zn concentration, 0, 25, and 50 μm, alone and/or in combination with 1 mm si. incremental zn concentration in growth media instigated the cellular oxidative damage ... | 2015 | 25516248 |
| a comparative meta-analysis of qtl between intraspecific gossypium hirsutum and interspecific g. hirsutum × g. barbadense populations. | based on 1075 and 1059 qtl from intraspecific upland and interspecific upland × pima populations, respectively, the identification of qtl clusters and hotspots provides a useful resource for cotton breeding. mapping of quantitative trait loci (qtl) is a pre-requisite of marker-assisted selection for crop yield and quality. recent meta-analysis of qtl in tetraploid cotton (gossypium spp.) has identified regions of the genome with high concentrations of qtl for various traits called clusters and s ... | 2015 | 25501533 |
| development of est-based snp and indel markers and their utilization in tetraploid cotton genetic mapping. | availability of molecular markers has proven to be an efficient tool in facilitating progress in plant breeding, which is particularly important in the case of less researched crops such as cotton. considering the obvious advantages of single nucleotide polymorphisms (snps) and insertion-deletion polymorphisms (indels), expressed sequence tags (ests) were analyzed in silico to identify snps and indels in this study, aiming to develop more molecular markers in cotton. | 2014 | 25442170 |
| identification and profiling of known and novel fiber micrornas during the secondary wall thickening stage in cotton (gossypium hirsutum) via high-throughput sequencing. | | 2014 | 25438700 |
| molecular cloning and functional analysis of the flowering locus t (ft) homolog ghft1 from gossypium hirsutum. | flowering locus t (ft) encodes a member of the phosphatidylethanolamine-binding protein (pebp) family that functions as the mobile floral signal, playing an important role in regulating the floral transition in angiosperms. we isolated an ft-homolog (ghft1) from gossypium hirsutum l. cultivar, xinluzao 33 ghft1 was predominantly expressed in stamens and sepals, and had a relatively higher expression level during the initiation stage of fiber development. ghft1 mrna displayed diurnal oscillations ... | 2015 | 25429737 |
| cotton (gossypium hirsutum l.). | cotton continues to be a crop of great economic importance in many developing and some developed countries. cotton plants expressing the bt gene to deter some of the major pests have been enthusiastically and widely accepted by the farmers in three of the major producing countries, i.e., china, india, and the usa. considering the constraints related to its production and the wide variety of products derived from the cotton plant, it offers several target traits that can be improved through genet ... | 2015 | 25416245 |
| ghdrin1, a novel drought-induced gene of upland cotton (gossypium hirsutum l.) confers abiotic and biotic stress tolerance in transgenic tobacco. | a novel stress tolerance cdna fragment encoding ghdrin1 protein was identified and its regulation was studied in cotton boll tissues and seedlings subjected to various biotic and abiotic stresses. phylogenetic and conserved domain prediction indicated that ghdrin1 was annotated with a hypothetical protein of unknown function. subcellular localization showed that ghdrin1 is localized in the chloroplasts. the promoter sequence was isolated and subjected to in silico study. various cis-acting eleme ... | 2015 | 25413882 |
| genome-wide analysis of the omega-3 fatty acid desaturase gene family in gossypium. | the majority of commercial cotton varieties planted worldwide are derived from gossypium hirsutum, which is a naturally occurring allotetraploid produced by interspecific hybridization of a- and d-genome diploid progenitor species. while most cotton species are adapted to warm, semi-arid tropical and subtropical regions, and thus perform well in these geographical areas, cotton seedlings are sensitive to cold temperature, which can significantly reduce crop yields. one of the common biochemical ... | 2014 | 25403726 |
| molecular markers and cotton genetic improvement: current status and future prospects. | narrow genetic base and complex allotetraploid genome of cotton (gossypium hirsutum l.) is stimulating efforts to avail required polymorphism for marker based breeding. the availability of draft genome sequence of g. raimondii and g. arboreum and next generation sequencing (ngs) technologies facilitated the development of high-throughput marker technologies in cotton. the concepts of genetic diversity, qtl mapping, and marker assisted selection (mas) are evolving into more efficient concepts of ... | 2014 | 25401149 |
| cloning of gossypium hirsutum sucrose non-fermenting 1-related protein kinase 2 gene (ghsnrk2) and its overexpression in transgenic arabidopsis escalates drought and low temperature tolerance. | the molecular mechanisms of stress tolerance and the use of modern genetics approaches for the improvement of drought stress tolerance have been major focuses of plant molecular biologists. in the present study, we cloned the gossypium hirsutum sucrose non-fermenting 1-related protein kinase 2 (ghsnrk2) gene and investigated its functions in transgenic arabidopsis. we further elucidated the function of this gene in transgenic cotton using virus-induced gene silencing (vigs) techniques. we hypoth ... | 2014 | 25393623 |
| global microrna modification in cotton (gossypium hirsutum l.). | micrornas (mirnas) are small noncoding rnas participating in versatile biological processes via post-transcriptionally gene regulation. however, how mirnas are modified or degraded remains unknown, despite years of studies have unravelled much details of mirna biogenesis and function. here, we systematically investigated mirna modification using six small rna sequencing libraries generated from cotton seedling as well as cotton fibre at five developmental stages. our results show that 1-2-nt tru ... | 2015 | 25393285 |
| morphological and physiological responses of cotton (gossypium hirsutum l.) plants to salinity. | salinization usually plays a primary role in soil degradation, which consequently reduces agricultural productivity. in this study, the effects of salinity on growth parameters, ion, chlorophyll, and proline content, photosynthesis, antioxidant enzyme activities, and lipid peroxidation of two cotton cultivars, [ccri-79 (salt tolerant) and simian 3 (salt sensitive)], were evaluated. salinity was investigated at 0 mm, 80 mm, 160 mm, and 240 mm nacl for 7 days. salinity induced morphological and ph ... | 2014 | 25391141 |
| genome-wide cloning, identification, classification and functional analysis of cotton heat shock transcription factors in cotton (gossypium hirsutum). | heat shock transcriptional factors (hsfs) play important roles in the processes of biotic and abiotic stresses as well as in plant development. cotton (gossypium hirsutum, 2n=4x=(ad)2=52) is an important crop for natural fiber production. due to continuous high temperature and intermittent drought, heat stress is becoming a handicap to improve cotton yield and lint quality. recently, the related wild diploid species gossypium raimondii genome (2n=2x=(d5)2=26) has been fully sequenced. in order t ... | 2014 | 25378022 |
| evaluation of cotton leaf curl virus resistance in bc1, bc2, and bc3 progenies from an interspecific cross between gossypium arboreum and gossypium hirsutum. | cotton leaf curl virus disease (clcud) is an important constraint to cotton production. the resistance of g. arboreum to this devastating disease is well documented. in the present investigation, we explored the possibility of transferring genes for resistance to clcud from g. arboreum (2n = 26) cv 15-mollisoni into g. hirsutum (2n = 52) cv crsm-38 through conventional breeding. we investigated the cytology of the bc1 to bc3 progenies of direct and reciprocal crosses of g. arboreum and g. hirsut ... | 2014 | 25372141 |
| cotton (gossypium hirsutum) 14-3-3 proteins participate in regulation of fibre initiation and elongation by modulating brassinosteroid signalling. | cotton (gossypium hirsutum) fibre is an important natural raw material for textile industry in the world. understanding the molecular mechanism of fibre development is important for the development of future cotton varieties with superior fibre quality. in this study, overexpression of gh14-3-3l in cotton promoted fibre elongation, leading to an increase in mature fibre length. in contrast, suppression of expression of gh14-3-3l, gh14-3-3e and gh14-3-3h in cotton slowed down fibre initiation and ... | 2015 | 25370928 |
| itraq protein profile differential analysis between somatic globular and cotyledonary embryos reveals stress, hormone, and respiration involved in increasing plantlet regeneration of gossypium hirsutum l. | somatic embryo development (sed) in upland cotton shows low frequencies of embryo maturation and plantlet regeneration. progress in increasing the regeneration rate has been limited. here a global analysis of proteome dynamics between globular and cotyledonary embryos was performed using isobaric tags for relative and absolute quantitation to explore mechanisms underlying sed. of 6318 proteins identified by a mass spectrometric analysis, 102 proteins were significantly up-regulated and 107 were ... | 2015 | 25367710 |
| development and bin mapping of gene-associated interspecific snps for cotton (gossypium hirsutum l.) introgression breeding efforts. | cotton (gossypium spp.) is the largest producer of natural fibers for textile and is an important crop worldwide. crop production is comprised primarily of g. hirsutum l., an allotetraploid. however, elite cultivars express very small amounts of variation due to the species monophyletic origin, domestication and further bottlenecks due to selection. conversely, wild cotton species harbor extensive genetic diversity of prospective utility to improve many beneficial agronomic traits, fiber charact ... | 2014 | 25359292 |
| identification of novel micrornas in the verticillium wilt-resistant upland cotton variety kv-1 by high-throughput sequencing. | plant micrornas (mirnas) play essential roles in the post-transcriptional regulation of gene expression during development, flowering, plant growth, metabolism, and stress responses. verticillium wilt is one of the vascular disease in plants, which is caused by the verticillium dahlia and leads to yellowing, wilting, lodging, damage to the vascular tissue, and death in cotton plants. upland cotton varieties kv-1 have shown resistance to verticillium wilt in multiple levels. however, the knowledg ... | 2014 | 25332864 |
| overexpression of a cotton annexin gene, ghann1, enhances drought and salt stress tolerance in transgenic cotton. | plant annexins are members of a diverse, multigene protein family that has been associated with a variety of cellular processes and responses to abiotic stresses. ghann1, which encodes a putative annexin protein, was isolated from a cotton (gossypium hirsutum l. acc 7235) cdna library. tissue-specific expression showed that ghann1 is expressed at differential levels in all tissues examined and strongly induced by various phytohormones and abiotic stress. in vivo and in vitro subcellular localiza ... | 2015 | 25330941 |
| functional characterization of cotton genes responsive to verticillium dahliae through bioinformatics and reverse genetics strategies. | verticillium wilt causes dramatic cotton yield loss in china. although some genes or biological processes involved in the interaction between cotton and verticillium dahliae have been identified, the molecular mechanism of cotton resistance to this disease is still poorly understood. the basic innate immune response for defence is somewhat conserved among plant species to defend themselves in complex environments, which makes it possible to characterize genes involved in cotton immunity based on ... | 2014 | 25326626 |
| mapping genomic loci for cotton plant architecture, yield components, and fiber properties in an interspecific (gossypium hirsutum l. × g. barbadense l.) ril population. | a quantitative trait locus (qtl) mapping was conducted to better understand the genetic control of plant architecture (pa), yield components (yc), and fiber properties (fp) in the two cultivated tetraploid species of cotton (gossypium hirsutum l. and g. barbadense l.). one hundred and fifty-nine genomic regions were identified on a saturated genetic map of more than 2,500 ssr and snp markers, constructed with an interspecific recombinant inbred line (ril) population derived from the genetic stan ... | 2014 | 25314923 |
| cotton lim domain-containing protein ghplim1 is specifically expressed in anthers and participates in modulating f-actin. | as one form of actin binding protein (abp), lim domain protein can trigger the formation of actin bundles during plant growth and development. in this study, a cdna (designated ghplim1) encoding a lim domain protein with 216 amino acid residues was identified from a cotton flower cdna library. quantitative rt-pcr indicated that ghplim1 is specifically expressed in cotton anthers, and its expression levels are regulated during anther development of cotton. ghplim1:egfp transformed cotton cells di ... | 2015 | 25294521 |
| the cotton wrky transcription factor ghwrky17 functions in drought and salt stress in transgenic nicotiana benthamiana through aba signaling and the modulation of reactive oxygen species production. | drought and high salinity are two major environmental factors that significantly limit the productivity of agricultural crops worldwide. wrky transcription factors play essential roles in the adaptation of plants to abiotic stresses. however, wrky genes involved in drought and salt tolerance in cotton (gossypium hirsutum) are largely unknown. here, a group iid wrky gene, ghwrky17, was isolated and characterized. ghwrky17 was found to be induced after exposure to drought, salt, h2o2 and aba. the ... | 2014 | 25261532 |
| [genome-wide analysis of hsp70 superfamily in gossypium raimondii and the expression of orthologs in gossypium hirsutum]. | heat shock 70 (hsp70) proteins are highly conserved molecular chaperones widely existed in the plant kingdom which are involved in cellular protein folding process. in this study, comprehensive evolutionary analyses of the gossypium raimondii hsp70 gene family members are conducted and 30 hsp70 genes are identified. the gene structure, chromosome distribution, gene duplication and phylogenic evolution of this family are further analyzed. the results reveal that hsp70 family genes can be clustere ... | 2014 | 25252310 |
| effects of climate change on phenological trends and seed cotton yields in oasis of arid regions. | understanding the effects of climatic change on phenological phases of cotton (gossypium hirsutum l.) in oasis of arid regions may help optimize management schemes to increase productivity. this study assessed the impacts of climatic changes on the phenological phases and productivity of spring cotton. the results showed that climatic warming led the dates of sowing seed, seeding emergence, three-leaf, five-leaf, budding, anthesis, full bloom, cleft boll, boll-opening, boll-opening filling, and ... | 2015 | 25240389 |
| genetic mapping of fiber color genes on two brown cotton cultivars in xinjiang. | in the present study, genetic linkage analysis was carried out to map the fiber color loci lc 1 and lc 2 on two brown cotton cultivars with ssr and est-ssr markers in the reference map by f2 segregation populations. the lc 1 locus carried by xincaimian6 (gossypium hirsutum l.) was flanked by the marker nau2862 and nau1043 on the long arm of chromosome 07, with genetic distance 7.8 cm and 3.8 cm, respectively. the lc 2 carried by xincaimian 5 (gossypium hirsutum l.) was flanked by the marker nau5 ... | 2014 | 25207203 |
| distribution and differentiation of wild, feral, and cultivated populations of perennial upland cotton (gossypium hirsutum l.) in mesoamerica and the caribbean. | perennial forms of gossypium hirsutum are classified under seven races. five mesoamerican races would have been derived from the wild race 'yucatanense' from northern yucatán. 'marie-galante', the main race in the caribbean, would have developed from introgression with g. barbadense. the racial status of coastal populations from the caribbean has not been clearly defined. we combined ecological niche modeling with an analysis of ssr marker diversity, to elucidate the relationships among cultivat ... | 2014 | 25198534 |
| comprehensive analysis of differentially expressed genes and transcriptional regulation induced by salt stress in two contrasting cotton genotypes. | cotton (gossypium spp.) is one of the major fibre crops of the world. although it is classified as salt tolerant crop, cotton growth and productivity are adversely affected by high salinity, especially at germination and seedling stages. identification of genes and mirnas responsible for salt tolerance in upland cotton (gossypium hirsutum l.) would help reveal the molecular mechanisms of salt tolerance. we performed physiological experiments and transcriptome sequencing (mrna-seq and small rna-s ... | 2014 | 25189468 |
| quantitative trait loci mapping and genetic dissection for lint percentage in upland cotton (gossypium hirsutum). | lint percentage is an important character of cotton yield components and it is also correlated with cotton fibre development. in this study, we used a high lint percentage variety, baimian1, and a low lint percentage, tm-1 genetic standard for gossypium hirsutum, as parents to construct a mapping populations in upland cotton (g. hirsutum). a quantitative trait locus/loci (qtl) analysis of lint percentage was performed by using two mapping procedures; composite interval mapping (cim), inclusive c ... | 2014 | 25189232 |
| ultrastructural, metabolic and proteomic changes in leaves of upland cotton in response to cadmium stress. | present study explores physiological, biochemical and proteomic changes in leaves of upland cotton (zms-49) using 500 μm cadmium (cd) along with control. leaves' biomass and chlorophyll pigments decreased at 500 μm cd. cd contents in roots were higher than leaves. levels of ros ( [formula: see text] and h2o2) both in vivo and in vitro and mda contents were significantly increased. chlorophyll parameters (f0, fm, fm(') and fv/fm), total soluble protein contents and apx showed a decline at 500 μm ... | 2015 | 25169734 |
| genomic organization, differential expression, and functional analysis of the spl gene family in gossypium hirsutum. | squamosa promoter binding protein-like (spl) genes encode plant-specific transcription factors that are involved in many fundamental developmental processes. certain spl genes contain sequences complementary to mir156, a microrna (mirna) that plays a role in modulating plant gene expression. in this study, 30 spl genes were identified in the reference genome of gossypium raimondii and 24 ghspls were cloned from gossypium hirsutum. g. raimondii is regarded as the putative contributor of the d-sub ... | 2015 | 25159110 |
| proteomics profiling of fiber development and domestication in upland cotton (gossypium hirsutum l.). | comparative proteomic analyses were performed to detail the evolutionary consequences of strong directional selection for enhanced fiber traits in modern upland cotton (gossypium hirsutum l.). using two complementary proteomic approaches, 2-de and itraq lc-ms/ms, fiber proteomes were examined for four representative stages of fiber development. approximately 1,000 protein features were characterized using each strategy, collectively resulting in the identification and functional categorization o ... | 2014 | 25156487 |
| water deficit in field-grown gossypium hirsutum primarily limits net photosynthesis by decreasing stomatal conductance, increasing photorespiration, and increasing the ratio of dark respiration to gross photosynthesis. | much effort has been expended to improve irrigation efficiency and drought tolerance of agronomic crops; however, a clear understanding of the physiological mechanisms that interact to decrease source strength and drive yield loss has not been attained. to elucidate the underlying mechanisms contributing to inhibition of net carbon assimilation under drought stress, three cultivars of gossypium hirsutum were grown in the field under contrasting irrigation regimes during the 2012 and 2013 growing ... | 2014 | 25151126 |
| a cotton (gossypium hirsutum) gene encoding a nac transcription factor is involved in negative regulation of plant xylem development. | nac proteins that compose of one large family of plant specific transcription factors (tf) play the important roles in many biological processes (such as morphogenesis, development, senescence and stress signal transduction). in this study, a gene (designated as ghxnd1) encoding a nac transcription factor was identified in cotton. sequence analysis indicated that ghxnd1 gene contains two introns inserted in its open reading frame (orf). ghxnd1 protein is localized in the cell nucleus, and displa ... | 2014 | 25137291 |
| effect of late planting and shading on cellulose synthesis during cotton fiber secondary wall development. | cotton-rapeseed or cotton-wheat double cropping systems are popular in the yangtze river valley and yellow river valley of china. due to the competition of temperature and light resources during the growing season of double cropping system, cotton is generally late-germinating and late-maturing and has to suffer from the coupling of declining temperature and low light especially in the late growth stage. in this study, late planting (lp) and shading were used to fit the coupling stress, and the ... | 2014 | 25133819 |
| next generation genetic mapping of the ligon-lintless-2 (li₂) locus in upland cotton (gossypium hirsutum l.). | mapping-by-sequencing and novel subgenome-specific snp markers were used to fine map the ligon-lintless 2 ( li 2 ) short-fiber gene in tetraploid cotton. these methodologies will accelerate gene identification in polyploid species. next generation sequencing offers new ways to identify the genetic mechanisms that underlie mutant phenotypes. the release of a reference diploid gossypium raimondii (d5) genome and bioinformatics tools to sort tetraploid reads into subgenomes has brought cotton genet ... | 2014 | 25119870 |
| transcriptome and complexity-reduced, dna-based identification of intraspecies single-nucleotide polymorphisms in the polyploid gossypium hirsutum l. | varietal single nucleotide polymorphisms (snps) are the differences within one of the two subgenomes between different tetraploid cotton varieties and have not been practically used in cotton genetics and breeding because they are difficult to identify due to low genetic diversity and very high sequence identity between homeologous genes in cotton. we have used transcriptome and restriction site-associated dna sequencing to identify varietal snps among 18 g. hirsutum varieties based on the ratio ... | 2014 | 25106949 |
| transcriptome analysis of extant cotton progenitors revealed tetraploidization and identified genome-specific single nucleotide polymorphism in diploid and allotetraploid cotton. | the most widely cultivated cotton (gossypium hirsutum l., ad-genome) is derived from tetraploidization between a- and d-genome species. g. arboreum l. (a-genome) and g. raimondii ulbr. (d-genome) are two of closely-related extant progenitors. gene expression studies in allotetraploid cotton are complicated by the homoeologous loci of a- and d-genome origins. to develop genomic resources for gene expression and cotton breeding, we sequenced and assembled expressed sequence tags (ests) derived fro ... | 2014 | 25099166 |
| molecular analysis of proanthocyanidins related to pigmentation in brown cotton fibre (gossypium hirsutum l.). | the structural characteristics and component differences of proanthocyanidins in brown and white cotton fibres were identified by nuclear magnetic resonance (nmr) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (maldi-tof ms) analyses. proanthocyanidins in brown and white cotton fibres were found to contain mainly procyanidin (pc) and prodelphidin (pd) units with 2, 3-cis form (epigallocatechin and epicatechin). however, part of the proanthocyanidins in the white ... | 2014 | 25086591 |
| the effect of mepiquat chloride on elongation of cotton (gossypium hirsutum l.) internode is associated with low concentration of gibberellic acid. | the growth regulator mepiquat chloride (mc) is globally used in cotton (gossypium hirsutum l.) canopy manipulation to avoid excess growth and yield loss. however, little information is available as to whether the modification of plant architecture by mc is related to alterations in gibberellic acid (ga) metabolism and signaling. here, the role of ga metabolism and signaling was investigated in cotton seedlings treated with mc. the mc significantly decreased endogenous ga3 and ga4 levels in the e ... | 2014 | 25017155 |
| in vitro cadmium-induced alterations in growth and oxidative metabolism of upland cotton (gossypium hirsutum l.). | cadmium (cd) is a toxic pollutant, which cause both dose- and time-dependent physiological and biochemical alterations in plants. the present in vitro study was undertaken to explore cd-induced physiological and biochemical changes in cotton callus culture at 0, 550, 700, 850, and 1000 μm cd for four different stress periods (7, 14, 21, and 28 days). at 1000 μm cd, mean growth values were lower than their respective control. the cell protein contents decreased only after 7-day and 14-day stress ... | 2014 | 25013851 |
| predicting the effects of environment and management on cotton fibre growth and quality: a functional-structural plant modelling approach. | in general, the quality of fruits depends on local conditions experienced by the fruit during its development. in cotton, fruit quality, and more specifically the quality of the fibre in the fruit, depends on interactions between fruit position in the plant architecture, temperature and agronomic practices, such as sowing time, mulching with plastic film and topping of the plant's main stem and branches. to quantify this response of cotton fibre quality to environment and management, we develope ... | 2014 | 25011385 |
| genome-wide analysis of the wrky gene family in cotton. | wrky proteins are major transcription factors involved in regulating plant growth and development. although many studies have focused on the functional identification of wrky genes, our knowledge concerning many areas of wrky gene biology is limited. for example, in cotton, the phylogenetic characteristics, global expression patterns, molecular mechanisms regulating expression, and target genes/pathways of wrky genes are poorly characterized. therefore, in this study, we present a genome-wide an ... | 2014 | 24942461 |
| novel method to establish molecular identity using inter-simple sequence repeat markers in cotton (gossypium hirsutum) cultivars. | plant molecular identity (id) is used to describe molecular characteristics of plants, which should contain all of the necessary information. using inter-simple sequence repeat (issr) primers, molecular id can be described in a way that reflects the polymerase chain reaction (pcr) conditions, annealing temperature, and the bands obtained in pcr amplification. a new complete molecular id system is described in this study, which can be easily used and expanded to include more information. using th ... | 2014 | 24938605 |
| protein expression changes during cotton fiber elongation in response to drought stress and recovery. | an investigation to better understand the molecular mechanism of cotton (gossypium hirsutum l.) fiber elongation in response to drought stress and recovery was conducted using a comparative proteomics analysis. cotton plants (cv. nucotn 33b) were subjected to water deprivation for 10 days followed by a recovery period (with watering) of 5 days. the temporal changes in total proteins in cotton fibers were examined using 2de. the results revealed that 163 proteins are significantly drought respons ... | 2014 | 24889071 |
| quantitative trait loci analysis of fiber quality traits using a random-mated recombinant inbred population in upland cotton (gossypium hirsutum l.). | upland cotton (gossypium hirsutum l.) accounts for about 95% of world cotton production. improving upland cotton cultivars has been the focus of world-wide cotton breeding programs. negative correlation between yield and fiber quality is an obstacle for cotton improvement. random-mating provides a potential methodology to break this correlation. the suite of fiber quality traits that affect the yarn quality includes the length, strength, maturity, fineness, elongation, uniformity and color. iden ... | 2014 | 24886099 |
| microrna-target gene responses to lead-induced stress in cotton (gossypium hirsutum l.). | micrornas (mirnas) play key roles in plant responses to various metal stresses. to investigate the mirna-mediated plant response to heavy metals, cotton (gossypium hirsutum l.), the most important fiber crop in the world, was exposed to different concentrations (0, 25, 50, 100, and 200 µm) of lead (pb) and then the toxicological effects were investigated. the expression patterns of 16 stress-responsive mirnas and 10 target genes were monitored in cotton leaves and roots by quantitative real-time ... | 2014 | 24879091 |
| ghagl15s, preferentially expressed during somatic embryogenesis, promote embryogenic callus formation in cotton (gossypium hirsutum l.). | somatic embryogenesis is a useful tool for gene transfer and propagation of plants. agamous-like15 (agl15) promotes somatic embryogenesis in many plant species. in this study, three homologous agl15 genes were isolated from gossypium hirsutum l., namely ghagl15-1, ghagl15-3, and ghagl15-4. their putative proteins contained a highly conserved mads-box dna-binding domain and a less conserved k domain. phylogenetic analysis suggested that the three ghagl15s clustered most closely with agl15 protein ... | 2014 | 24833045 |
| radiation and chemical mutagen induced somaclonal variations through in vitro organogenesis of cotton (gossypium hirsutum l.). | the purpose of the investigation was to induce somaclonal variations by gamma rays (gr), ethylmethane sulphonate (ems) and sodium azide (sa) during in vitro organogenesis of cotton. | 2014 | 24831496 |
| cotton knl1, encoding a class ii knox transcription factor, is involved in regulation of fibre development. | in this study, the ghknl1 (knotted1-like) gene, encoding a classical class ii knox protein was identified in cotton (gossypium hirsutum). ghknl1 was preferentially expressed in developing fibres at the stage of secondary cell wall (scw) biosynthesis. ghknl1 was localized in the cell nucleus, and could interact with ghofp4, as well as atofp1, atofp4, and atmyb75. however, ghknl1 lacked transcriptional activation activity. dominant repression of ghknl1 affected fibre development of cotton. the exp ... | 2014 | 24831118 |
| association mapping for epistasis and environmental interaction of yield traits in 323 cotton cultivars under 9 different environments. | improving yield is a major objective for cotton breeding schemes, and lint yield and its three component traits (boll number, boll weight and lint percentage) are complex traits controlled by multiple genes and various environments. association mapping was performed to detect markers associated with these four traits using 651 simple sequence repeats (ssrs). a mixed linear model including epistasis and environmental interaction was used to screen the loci associated with these four yield traits ... | 2014 | 24810754 |
| functional characterization of ghakt1, a novel shaker-like k⁺ channel gene involved in k⁺ uptake from cotton (gossypium hirsutum). | shaker-like potassium (k(+)) channels in plants play an important role in k(+) absorption and transport. in this study, we characterized a shaker-like k(+) channel gene ghakt1 from the roots of gossypium hirsutum cv. liaomian17. phylogenetic analysis showed that the ghakt1 belongs to the akt1-subfamily in the shaker-like k(+) channel family. confocal imaging of a ghakt1-green fluorescent fusion protein (gfp) in transgenic arabidopsis plants indicated that ghakt1 is localized in the plasma membra ... | 2014 | 24802116 |
| pag1, a cotton brassinosteroid catabolism gene, modulates fiber elongation. | cotton (gossypium hirsutum) is the major source of natural textile fibers. brassinosteroids (brs) play crucial roles in regulating fiber development. the molecular mechanisms of brs in regulating fiber elongation, however, are poorly understood. pagoda1 (pag1) was identified via an activation tagging genetic screen and characterized by genome walking and brassinolide (bl) supplementation. rna-seq analysis was employed to elucidate the mechanisms of pag1 in regulating fiber development. pag1 exhi ... | 2014 | 24786710 |
| molecular mapping and validation of a major qtl conferring resistance to a defoliating isolate of verticillium wilt in cotton (gossypium hirsutum l.). | verticillium wilt (vw) caused by verticillium dahliae kleb is one of the most destructive diseases of cotton. development and use of a vw resistant variety is the most practical and effective way to manage this disease. identification of highly resistant genes/qtl and the underlining genetic architecture is a prerequisite for developing a vw resistant variety. a major qtl qvw-c6-1 conferring resistance to the defoliating isolate v991 was identified on chromosome 6 in lhb22×jm11 f2∶3 population i ... | 2014 | 24781706 |
| lack of adaptation to a new host in a generalist herbivore: implications for host plant resistance to twospotted spider mites in cotton. | the twospotted spider mite (tetranychus urticae koch) is an important pest of cotton. this pest has a broad host range, but when changing between hosts an initial decline in fitness often occurs. this is usually followed by an increase in fitness after rapid adaptation to the new host, usually within five generations. | 2015 | 24777962 |
| independent replication of mitochondrial genes supports the transcriptional program in developing fiber cells of cotton (gossypium hirsutum l.). | the mitochondrial genomes of flowering plants exist both as a "master circle" chromosome and as numerous subgenomic sublimons that are generated by intramolecular recombination. differential stability or replication of these sublimons allows individual mitochondrial gene copy numbers to vary independently between different cell types and developmental stages. our objective was to determine the relationship between mitochondrial gene copy number and transcript abundance in the elongating fiber ce ... | 2014 | 24768176 |
| waterlogging during flowering and boll forming stages affects sucrose metabolism in the leaves subtending the cotton boll and its relationship with boll weight. | the work explored sucrose metabolism in the leaves subtending the cotton boll (sbl) and its role in boll weight after waterlogging in cotton. results showed that net photosynthesis rate (pn), relative water content, contents of chlorophyll a and chlorophyll b, initial ribulose-1,5-bisphosphate carboxylase-oxygenase (rubisco) activity and cytosolic fructose-1, 6-bisphosphatase (cy-fbpase) activity decreased with waterlogging in the sbl on fruiting branches 2-3 (fb2-3) and fb6-7. activities of suc ... | 2014 | 24767118 |
| comparative transcriptome analysis of short fiber mutants ligon-lintless 1 and 2 reveals common mechanisms pertinent to fiber elongation in cotton (gossypium hirsutum l.). | understanding the molecular processes affecting cotton (gossypium hirsutum) fiber development is important for developing tools aimed at improving fiber quality. short fiber cotton mutants ligon-lintless 1 (li1) and ligon-lintless 2 (li2) are naturally occurring, monogenic mutations residing on different chromosomes. both mutations cause early cessation in fiber elongation. these two mutants serve as excellent model systems to elucidate molecular mechanisms relevant to fiber length development. ... | 2014 | 24748059 |
| ghmpk17, a cotton mitogen-activated protein kinase, is involved in plant response to high salinity and osmotic stresses and aba signaling. | mitogen-activated protein kinase (mapk) cascades play pivotal roles in mediating biotic and abiotic stress responses. cotton (gossypium hirsutum) is the most important textile crop in the world, and often encounters abiotic stress during its growth seasons. in this study, a gene encoding a mitogen-activated protein kinase (mapk) was isolated from cotton, and designated as ghmpk17. the open reading frame (orf) of ghmpk17 gene is 1494 bp in length and encodes a protein with 497 amino acids. quanti ... | 2014 | 24743296 |
| within-field spatial distribution of stink bug (hemiptera: pentatomidae)-induced boll injury in commercial cotton fields of the southeastern united states. | spatial distribution of boll injury caused by stink bugs to developing cotton (gossypium hirsutum l.) bolls was studied in five commercial fields (≍22 ha each) in 2011 and 2012 to understand variability in boll injury dynamics within fields. cotton bolls and stink bugs were sampled weekly from a georeferenced grid of sampling points (one sample per 0.40 ha) in each field, but no samples were taken within 30 m of field edges. the inverse distance weighted interpolation, variogram analysis, and mo ... | 2014 | 24735500 |
| quantitative phosphoproteomics analysis of nitric oxide-responsive phosphoproteins in cotton leaf. | knowledge of phosphorylation events and their regulation is crucial to understanding the functional biology of plant proteins, but very little is currently known about nitric oxide-responsive phosphorylation in plants. here, we report the first large-scale, quantitative phosphoproteome analysis of cotton (gossypium hirsutum) treated with sodium nitroprusside (nitric oxide donor) by utilizing the isobaric tag for relative and absolute quantitation (itraq) method. a total of 1315 unique phosphopep ... | 2014 | 24714030 |
| [effects of fertilization on cotton growth and nitrogen use efficiency under salinity stress]. | cotton (gossypium hirsutum) was raised at different salinity levels (0, 0.15% and 0.30%) by irrigating with fresh- or sea-water. the effects of fertilization (n, nk, np and npk) on plant growth, nitrogen (n) uptake and n use efficiency were studied. the results showed that salinity and fertilization both affected the biomass, agronomic n use efficiency, n bioavailability and nitrogen accumulation of plants, and significant interaction was observed between the two factors. fertilization could imp ... | 2013 | 24697064 |
| seed-specific expression of a lysine-rich protein gene, ghlrp, from cotton significantly increases the lysine content in maize seeds. | maize seed storage proteins are a major source of human and livestock consumption. however, these proteins have poor nutritional value, because they are deficient in lysine and tryptophan. much research has been done to elevate the lysine content by reducing zein content or regulating the activities of key enzymes in lysine metabolism. using the naturally lysine-rich protein genes, sb401 and sbglr, from potato, we previously increased the lysine and protein contents of maize seeds. here, we exam ... | 2014 | 24681583 |
| spectral reflectance properties of gossypium hirsutum leaves after heavy metal toxicity. | the study demonstrated a link between heavy metal induced stress and optical properties of the leaves of gossypium hirsutum. this work was conducted using the pot culture experiment. three replications and four concentrations of chromium (5%, 10%, 20% and 35%) with a control to assess the growth of plants were used. this experiment was conducted till 80 days. on 80th day the experiment was terminated and the leaves were plucked and air-dried. portion of the air-dried, powdered cotton leaves was ... | 2014 | 26563065 |
| dynamics of soil diazotrophic community structure, diversity, and functioning during the cropping period of cotton (gossypium hirsutum). | the soil sampled at different growth stages along the cropping period of cotton were analyzed using various molecular tools: restriction fragment length polymorphism (rflp), terminal restriction length polymorphism (t-rflp), and cloning-sequencing. the cluster analysis of the diazotrophic community structure of early sampled soil (0, 15, and 30 days) was found to be more closely related to each other than the later sampled one. phylogenetic and diversity analysis of sequences obtained from the f ... | 2015 | 24677076 |
| a peptide hormone gene, ghpsk promotes fibre elongation and contributes to longer and finer cotton fibre. | cotton fibres, the single-celled trichomes derived from the ovule epidermis, provide the most important natural material for the global textile industry. a number of studies have demonstrated that regulating endogenous hormone levels through transgenic approaches can improve cotton fibre qualities. phytosulfokine-α (psk-α) is a novel peptide hormone in plants that is involved in regulating cell proliferation and elongation. however, its potential applications in crop genetic improvement have not ... | 2014 | 24666593 |
| genetic dissection of upland cotton (gossypium hirsutum) cultivars developed in hubei province by mapped ssrs. | the genetic diversity of 51 upland cotton cultivars with different parental origins and breeding periods that were developed in hubei province was studied on the basis of 237 mapped simple sequence repeat markers covering the cotton genome. a total of 108 polymorphic primer pairs amplified 196 loci; the polymorphism information content range was 0.04 to 0.83, with an average of 0.46. a model-based clustering analysis (structure) of the genomic data identified 3 clear subpopulations, and the resu ... | 2014 | 24615042 |