| association of mycoplasma hominis and ureaplasma urealyticum with some indicators of nonspecific vaginitis. | the purpose of this study was to determine the isolation rates of mycoplasma hominis and ureaplasma urealyticum from three populations of women and also to relate the presence of these microorganisms with some indicators of nonspecific vaginitis. three hundred vaginal swabs were taken from delivery, pregnant and control (not pregnant) women. cultures were done in e broth supplemented with arginine or urea. m. hominis was isolated in 5% at delivery, 12% from pregnant and 5% from control women and ... | 2000 | 10948822 |
| role of the male partner in the lower genitourinary tract infection of female. | we studied the relationships of selected microbes and the role of consorts in the causation of vaginal discharge which may be due to cervicitis or vaginitis. a total of 93 consecutive patients in the reproductive age group with symptoms of vaginal discharge along with their sexual partners were studied. samples were collected from the cervix and posterior fornix of the female patients and from the urethra and sub-prepucial area of the male partner for culture of neisseria gonorrhoeae, gardnerell ... | 2000 | 11006655 |
| bacterial vaginosis. | bacterial vaginosis is the most common cause of vaginitis, affecting over 3 million women in the united states annually. depopulation of lactobacilli from the normal vaginal flora and overgrowth of gardnerella vaginalis and other anaerobic species are the presumed etiology. to date, no scientific evidence shows that bacterial vaginosis is a sexually transmitted disease. malodorous vaginal discharge is the most common symptom. differential diagnoses include trichomoniasis, moniliasis, and allergi ... | 2000 | 11025268 |
| binding of catalase by gardnerella vaginalis. | previous work has demonstrated that gardnerella vaginalis can utilize catalase as a sole source of iron. in this study, the interaction between g. vaginalis cells and catalase was investigated. g. vaginalis cells were shown to bind digoxigenin (dig)-labeled catalase using a solid phase dot blot assay. an increase in catalase binding was observed from cells grown under iron-restrictive conditions. western blot analysis of g. vaginalis proteins resulted in the detection of a putative catalase-bind ... | 2000 | 11034278 |
| [verification of identification of infectious agents by culture in routine gynecologic cytological screening]. | the original bethesda classification system for reporting cervical/vaginal cytologic diagnoses has claimed besides oncologic evaluation also a statement on the presence of infectious agents. their diagnosis should be followed by appropriate treatment. based upon the comparison of careful bacterioscopic study in a series of 175 routine cervical smears with the results of microbiological, virological and mycological examinations the following pathogens might be-according to the authors' opinion-di ... | 1999 | 11038666 |
| conservation in probiotic preparations of lactobacillus with inhibitory capacity on other species. | strains of lactobacillus isolated from dairy products and genital tract competed with candida albicans through a membrane of 12000 dalton cut-off. this inhibition was due to hydrogen peroxide and was trypsin-stable, heat-sensitive and antagonized by catalase. lactobacillus coming from "starters" showed antimicrobial activity against fungus isolated in a yogurt factory. penicillium, alternaria, phialophora, microsporum and candida spp. were inhibited when 10(2) spores were inoculated in the assay ... | 2000 | 11061631 |
| inhibition of chemotaxis by organic acids from anaerobes may prevent a purulent response in bacterial vaginosis. | it has been postulated that certain organic acids produced by the anaerobes associated with bacterial vaginosis (bv) could prevent a purulent response in this infection. varying concentrations of pure succinic, acetic and lactic acids were incubated in vitro with a monocytic cell line (monomac 6). high inhibition of chemotaxis was produced by succinic acid; lower inhibition and no inhibition was shown by acetic acid and lactic acid respectively. succinic and acetic acids were detected in high co ... | 2000 | 11073156 |
| adverse obstetric sequelae of bacterial vaginosis. | bacterial vaginosis is increasingly associated with adverse sequelae in obstetrics such as preterm prelabour rupture of membranes, preterm labour and preterm birth. it is important to diagnose the condition early in pregnancy where appropriate treatment can be administered to women who are symptomatic or who are at high risk of adverse sequelae. | 2000 | 11091802 |
| detection of gardnerella vaginalis in the vagina and amniotic fluid using the polymerase chain reaction. | | 2000 | 11102607 |
| [the production of a latex immunoglobulin conjugate for the diagnosis of gardnerella vaginalis]. | to obtain an anti-gadnerella vaginalis latex globulin, specific immunosera to reference strain (atcc 14018) and to a clinical isolation strain pool were produced. these immunosera were characterized using page-sds and immunoblotting where a close antigenic relation between the clinical isolation strains and the reference strain was observed. the latex globulin conjugates obtained from these 2 immunosera were evaluated in vitro with a resulting level of detection of 10 ufc/ml of gardnerella vagin ... | 2000 | 11107902 |
| [maternal-fetal infection with gardnerella vaginalis]. | | 2000 | 11109955 |
| identification of a gardnerella vaginalis hemoglobin-binding protein. | previous studies have shown that gardnerella vaginalis can utilize human hemoglobin as a sole source of iron. in this study, the interaction between human hemoglobin and g. vaginalis cells was investigated. with a solid phase dot blot assay, g. vaginalis cells were shown to bind digoxigenin (dig)-labeled human hemoglobin. a human hemoglobin-binding protein with an estimated molecular weight of 124 kilodaltons (kda) was detected by western blot analysis of g. vaginalis proteins. the hemoglobin-bi ... | 2001 | 11116397 |
| a randomised controlled trial of vaginal clindamycin for early pregnancy bacterial vaginosis. | to determine whether treatment of bacterial vaginosis (bv) with vaginal clindamycin affects pregnancy outcome. | 2000 | 11117774 |
| characterization of the 16s rrna gene v2 region and the rrn operons of gardnerella vaginalis. | ribosomal rna (rrna) gene polymorphism was apparent when gardnerella vaginalis dna restriction profiles were hybridized with nonradioactively labeled total rrna isolated from this bacterium. in contrast, use of a polymerase chain reaction (pcr)-based 16s rrna gene v2 region resulted in a 118-bp v2-pcr amplicon that was specific and common in all 30 tested g. vaginalis isolates. in addition to providing a g. vaginalis-specific fingerprint, when the v2-pcr amplicon along with total rrna were utili ... | 2000 | 11130865 |
| a study on the possible association of dysfunctional uterine bleeding with bacterial vaginosis, mycoplasma, ureaplasma, and gardnerella vaginalis. | | 2000 | 11141864 |
| modern methods for diagnosis of gardnerella infection. | comparison of morphological, bacteriological, serological, and genetic methods for diagnosis of gardnerella infection in vaginosis showed that the first three methods are preferable, while the genetic method (polymerase chain reaction) is more efficient for identification of gardnerella vaginalis in culture. | 2000 | 11177243 |
| understanding the bacterial flora of the female genital tract. | the microbiological flora of the lower female genital tract provides a dynamic, complex example of microbial colonization, the regulation of which is not fully understood. when an exogenous bacterial species, with its array of virulence factors, is introduced into the host, disease does not always occur. conversely, under selected conditions, commensal endogenous bacteria-for example, gardnerella vaginalis and group b streptococci-can participate in disease processes. disease caused by both exog ... | 2001 | 11181139 |
| detection of cervical immunoglobulin a in normal pregnancy. | the aim of our study was to evaluate a feasible method to quantify the immunoglobulin a concentration in the cervical mucus of women with a normal singleton pregnancy. in 60 immunologic healthy pregnant women cervical mucus samples were taken at a random time in pregnancy using an absorbing cylindrical cotton-swab stick. in this cervical mucus immunoglobulin a concentration was measured by radial immunodiffusion. a vaginal bacterial swab was taken from each woman. concentration of immunoglobulin ... | 2001 | 11234615 |
| aerobic microbiological study in term pregnant women with premature rupture of the membranes: a case-control study. | to determine the aerobic microorganisms related to premature rupture of the membranes (prom) in term pregnant women, a case-controlled study was performed on pregnant women delivered at rajavithi hospital between november 1, 1996 and july 30, 1997. two hundred and twenty pregnant women with prom and 220 pregnant women without prom were recruited by simple random sampling. the diagnosis of rupture of the membrane was made by history and by positive microscopic ferning and ph testing performed dur ... | 2001 | 11281495 |
| predictors of the vaginal microflora. | our purpose was to define influences on the patterns of the vaginal microflora. | 2001 | 11303191 |
| binding of heme by gardnerella vaginalis. | it has been previously demonstrated that gardnerella vaginalis could acquire iron from a number of different iron-containing compounds, including heme. in this study, the direct binding of heme by g. vaginalis strains was demonstrated utilizing a liquid broth heme-binding assay. competition studies demonstrated that pretreatment of g. vaginalis cells with other iron sources such as hemoglobin, catalase, and lactoferrin did not affect heme binding. also, heme binding was not inhibited by preincub ... | 2001 | 11314245 |
| clarithromycin-induced alterations in vaginal flora. | many antibiotics have been shown to alter both the bacterial and the fungal flora of the vagina, in some cases potentially increasing a woman's propensity toward urinary tract infections and vaginal candidiasis. the effects of some of the newer macrolide antibiotics on women's vaginal flora have not been previously studied, and almost none of the previous studies specifically cultured for effects on vaginal lactobacillus. young women (ages 18-45 years) who were about to go onto therapy with clar ... | 2000 | 11317173 |
| the diagnosis of bacterial vaginosis and vaginal flora changes. | in a population of 956 women, attending for contraceptive advice, 131 (13.7%) were found to have bv acording to amsel's criteria. clue cells were detected in 200 (20.9%) women, a positive amine ('sniff') test in 191 (20.0%), a vaginal ph > or = 4.7 in 243 (25.4%) and a "characteristic" vaginal discharge in 104 (10.9%) women. sensitivity, specificity, positive and negative predictive values in relation to bv were calculated for each of these four criteria. the detection of clue cells, an increase ... | 2001 | 11327086 |
| vaginal candidiasis and other types of vaginitis. | the causes, diagnosis, treatment options, and recent research findings of vaginal infections in hiv-infected women are discussed. three common types of vaginitis are addressed: vaginal candidiasis, bacterial vaginosis, and trichomoniasis. the prevalence of candidiasis is an important indicator of hiv disease severity and data is provided that shows its frequency, compared to other genital infections, in hiv-positive women. | 1995 | 11362888 |
| [complications of sexually transmitted diseases: clinical course and treatment]. | sexually transmitted infectious agents cause a wide array of complications in the short-, medium-, and long-term, especially among women. specifically, infection with these agents can result in sterility, infertility, genital neoplasia, pre-term delivery, and foetal/neonatal pathologies, and the agents most commonly associated with these complications are: neisseria gonorrhoeae, chlamydia trachomatis, anaerobic bacteria, ureaplasma urealyticum, gardnerella vaginalis and human papilloma virus (hp ... | 2000 | 11367920 |
| vaginal microflora associated with bacterial vaginosis in nonpregnant women: reliability of sialidase detection. | to determine the prevalence of gardnerella vaginalis, anaerobic bacteria and mycoplasma hominis in vaginal specimens of women with and without bacterial vaginosis (bv) as well as to determine the sensitivity and specificity of the direct sialidase assay of vaginal fluid as a rapid test for diagnosing this syndrome. | 2001 | 11368254 |
| antimicrobial protein produced by vaginal lactobacillus acidophilus that inhibits gardnerella vaginalis. | to isolate bacteriocin from a vaginal strain of lactobacillus acidophilus. | 2001 | 11368257 |
| bacterial vaginosis among a group of married jordanian women: occurrence and laboratory diagnosis. | a total of 310 vaginal swabs collected from a group of married jordanian women complaining of vaginal discharge were examined for bacterial vaginosis. the scoring system of nugent for the interpretation of gram staining was employed. this system revealed the presence of the condition in 29.7% of patients. results obtained using the scoring system correlated significantly with the detection of clue cells and the scarcity of white blood cells in the vaginal discharge. an inverse relationship was f ... | 2001 | 11368266 |
| human immunodeficiency virus type 1 stimulatory activity by gardnerella vaginalis: relationship to biotypes and other pathogenic characteristics. | stimulation of human immunodeficiency virus (hiv) type 1 expression by gardnerella vaginalis is one possible cause for an increase in the amount of virus in the genital tract. the ability of g. vaginalis to induce hiv expression in chronically infected u1 cells was investigated, along with its possible relationship to biotype, genotype, and resistance to metronidazole and bacteriocin. significant hiv stimulatory activity was found in 5 (50%) lysates of g. vaginalis. the ability to induce hiv exp ... | 2001 | 11398105 |
| nugent score related to vaginal culture in pregnant women. | to relate gram-stained smears, using the nugent criteria, to quantitative and qualitative vaginal cultures in pregnant women. | 2001 | 11430961 |
| defense factors of vaginal lactobacilli. | to determine the antagonistic relationship between vaginal lactobacilli and endogenous vaginal microflora. | 2001 | 11518895 |
| evaluation of the affirm ambient temperature transport system for the detection and identification of trichomonas vaginalis, gardnerella vaginalis, and candida species from vaginal fluid specimens. | the objective of this study was to measure the performance of the affirm ambient temperature transport system (atts) over time and to estimate the length of time the system can preserve a vaginal specimen containing the three common organisms causing vaginitis: trichomonas vaginalis, candida species, and gardnerella vaginalis (one of the causative agents of bacterial vaginosis). women with symptoms of vaginitis presenting to one of three clinical centers were evaluated over a 4- to 8-week period ... | 2001 | 11526150 |
| gardnerella vaginalis isolated from patients with bacterial vaginosis and from patients with healthy vaginal ecosystems. | the differences in the phenotype and genotype of gardnerella vaginalis isolates from patients with bacterial vaginosis (bv) and from patients without bv are unknown. in our study, 43 isolates of g. vaginalis were examined for biotype (hippurate hydrolysis, lipase, and beta-galactosidase activity), sensitivity to metronidazole, and genotype. of the 117 women visiting the gynecology clinic at rush-presbyterian-st. luke's medical center who were included in the study, 27.4% were found to have bv. g ... | 2001 | 11528575 |
| relationship of abnormal vaginal flora, proinflammatory cytokines and idiopathic infertility in women undergoing ivf. | to investigate the prevalence of bacterial vaginosis (bv) and abnormal bacterial vaginal flora in an infertile population and correlate with cervical cytokine production and in vitro fertilization (ivf) outcome. | 2001 | 11584481 |
| [frequency of gardnerella vaginalis vaginosis and its association with other pathogens causing genital infections in the female]. | in order to determine the presence of unspecific vaginosis and their causes, 700 vaginal smears were obtained from patients assisting to the clinical laboratory of the familiar medicine unit no. 28 "gabriel mancera" of the instituto mexicano del seguro social, during 6 months. the patients age was from 18 to 55 years old. to establish the infectious etiology of these pathologies the vaginal smears were observed freshly and stained by gram's method. specific test for differentiate gardnerella vag ... | 2001 | 11588810 |
| new approach to phylogenetic analysis of the genus bifidobacterium based on partial hsp60 gene sequences. | the partial 60 kda heat-shock protein (hsp60) genes of 36 bifidobacterium strains representing 30 different bifidobacterium species and subspecies and of the type strain of gardnerella vaginalis were cloned and sequenced using a pair of universal degenerate hsp60 pcr primers. the hsp60 dna sequence similarities were determined for the taxa at various ranks as follows: 99.4-100% within the same species, 96% at the subspecies level, and 73-96% (mean 85%) at the interspecies level (and 98% in the c ... | 2001 | 11594590 |
| antimicrobial therapy of infections with aerobic gram-positive rods. | this review presents data on in vitro susceptibilities of aerobically growing gram-positive rods and in vivo activities of antibiotics used against gram-positive rods. while in some instances susceptibility and efficacy are predictable (e.g. penicillin vs. listeria and microaerophilic coryneforms, or metronidazole vs. gardnerella) susceptibility testing by dilution techniques seems necessary for many gram-positive rods as long as they are deemed clinically relevant. | 2001 | 11688533 |
| bacteriocin susceptibility of gardnerella vaginalis and its relationship to biotype, genotype, and metronidazole susceptibility. | our purpose was to investigate the bacteriocin susceptibility of gardnerella vaginalis and its relationship to biotype, genotype, and resistance to metronidazole. | 2001 | 11717655 |
| gluteal necrotizing myofascitis: an unusual delayed complication of abdominal sacrocolpopexy. | persistent low back pain developed in a 51-year-old woman after she had undergone abdominal sacrocolpopexy. four months postoperatively, necrotizing myofascitis developed in her gluteal muscles. the infected mesh, which had eroded into the vagina, was removed. cultures of the infected mesh and abscesses grew common vaginal flora, including gardnerella vaginalis and actinomyces. | 2001 | 11717673 |
| frequency of trichomonas vaginalis, candida sp and gardnerella vaginalis in cervical-vaginal smears in four different decades. | vaginitis is one of the principal motives that lead women to seek out an obstetrician or gynecologist. bacterial vaginosis, candidiasis and trichomoniasis are responsible for 90% of the cases of infectious vaginitis. | 2001 | 11723534 |
| vaginitis emphysematosa associated with an abnormal pap smear. | vaginitis emphysematosa is an uncommon inflammatory condition that is aetiologically linked to trichomonal or gardnerella infection, and has been associated with immunosuppressive disorders. the disease does not have deleterious sequelae and resolves on treating the underlying infection. we describe a case in which the disease predominantly affected the cervix leading to an abnormal pap smear and colposcopic investigation. | 2001 | 11769034 |
| intravaginal bacterial flora in patients with uterine cervical cancer. high incidence of detection of gardnerella vaginalis. | gynecological diseases may affect the growth of vaginal bacterial flora. we investigated the relationship between uterine cervical cancer and the vaginal bacterial flora. in 20 patients with uterine cervical cancer, we investigated the intravaginal bacterial flora, including gardnerella vaginalis and mobiluncus spp. in the patients with uterine cervical cancer, there was a mixed flora of aerobes and anaerobes and, of special note, g. vaginalis was detected in 50% of the patients with uterine cer ... | 1999 | 11810495 |
| vaginal microflora in healthy women with gardnerella vaginalis. | in order to find the vaginal prevalence of gardnerella vaginalis in a normal female population, we determined the incidence of g. vaginalis in relation to that of other bacterial genera and species in the vagina. two-hundred and thirty-nine healthy women were the subjects of this study. vaginal discharge was collected and bacteriological studies were performed. the mean total aerobe count in the g. vaginalis-positive group was 9.02 log10 colony-forming units (cfu)/g, which was significantly high ... | 2000 | 11810560 |
| [colpocytology in a preventive gynecological ambulatory service]. | evaluate the results of pap smear in women attended at a gynecology preventive ambulatory (university of são paulo medical school clinical hospital). | 2001 | 11813045 |
| [genital infections of women. leukorrhea]. | | 2001 | 11828643 |
| in vitro antagonistic effect of lactobacillus on organisms associated with bacterial vaginosis. | to assess antagonistic properties of lactobacillus strains isolated from the vaginas of healthy women as compared to the most common bacterial agents related to vaginosis. | 2002 | 11838310 |
| definition of a type of abnormal vaginal flora that is distinct from bacterial vaginosis: aerobic vaginitis. | to define an entity of abnormal vaginal flora: aerobic vaginitis. | 2002 | 11845812 |
| a study to detect gardnerella vaginalis dna in interstitial cystitis. | to investigate the possible role of gardnerella vaginalis in interstitial cystitis (ic), using molecular methods to avoid difficulties with the culture and recovery of viable organisms, and the problems associated with the recovery of low numbers of culturable organisms. | 2001 | 11851605 |
| validity of wet-mount bacterial morphotype identification of vaginal fluid by phase-contrast microscopy for diagnosis of bacterial vaginosis in family practice. | the aim of this study was to determine the accuracy of a wet-mount bacterial morphology scoring (bms) system and nugent's gram stain analysis for the diagnosis of bacterial vaginosis, using amsel's criteria as the gold standard. the three diagnostic criteria were assessed independently the bms diagnosis was based on a scoring system which weighed the number of small bacterial morphotypes regarded as typical of bacterial vaginosis against lactobacillary morphotypes in phase-contrast microscopy of ... | 2001 | 11878711 |
| models of white matter injury: comparison of infectious, hypoxic-ischemic, and excitotoxic insults. | white matter damage (wmd) in preterm neonates is strongly associated with adverse outcome. the etiology of white matter injury is not known but clinical data suggest that ischemia-reperfusion and/or infection-inflammation are important factors. furthermore, antenatal infection seems to be an important risk factor for brain injury in term infants. in order to explore the pathophysiological mechanisms of wmd and to better understand how infectious agents may affect the vulnerability of the immatur ... | 2002 | 11921384 |
| [frequency of vaginosis caused by gardnerella vaginalis]. | | 2002 | 11924566 |
| characterization of vaginal flora and bacterial vaginosis in women who have sex with women. | bacterial vaginosis (bv) may be common among women who report having sex with women (wsw) and frequently occurs in both members of monogamous couples. the results of gram staining of a vaginal smear were consistent with bv in 81 (25%) and intermediate in 37 (11%) of 326 wsw included in this study. lactobacilli were detected in 64% of subjects, and 42% of subjects had h(2)o(2)-producing strains. bv was associated with a higher lifetime number of female sex partners, failure to always clean an ins ... | 2002 | 12001048 |
| efficacy of povidone-iodine vaginal suppositories in the treatment of bacterial vaginosis. | a prospective, randomized clinical trial was performed to study the efficacy of povidone iodine (betadine) suppositories for the treatment of bacterial vaginosis (bv) in comparison to capsules containing lactobacilli (döderlein med). seventy patients with bv included in the study were randomly assigned to be either treated with povidone iodine suppositories or lactobacilli. patients were treated once a day for 5 days. initial examinations took place on the first day of the study with follow-up e ... | 2002 | 12011527 |
| correlation of local interleukin-8 with immunoglobulin a against gardnerella vaginalis hemolysin and with prolidase and sialidase levels in women with bacterial vaginosis. | mucosal immune system activation may represent a critical determinant of adverse consequences associated with bacterial vaginosis (bv), such as sexual human immunodeficiency virus transmission, upper genital tract infections, postsurgical infections, and adverse pregnancy outcomes. concentrations of sialidase, prolidase, and anti-gardnerella vaginalis hemolysin (gvh) immunoglobulin a (iga) were higher in vaginal fluids of 75 fertile women with bv, compared with concentrations in vaginal fluids o ... | 2002 | 12023767 |
| [cervicovaginal infection as a risk factor for premature labor]. | to identify the possible association between cervicovaginal infections (cvi) and preterm delivery. | 2002 | 12050955 |
| rates of bacterial vaginosis in women undergoing in vitro fertilisation for different types of infertility. | to assess whether the rate of bacterial vaginosis (bv) is higher in women with tubal factor infertility compared with those with other causes of infertility. | 2002 | 12118653 |
| two novel vaginal microbicides (polystyrene sulfonate and cellulose sulfate) inhibit gardnerella vaginalis and anaerobes commonly associated with bacterial vaginosis. | this is the first report demonstrating the in vitro inhibitory activity of two novel microbicides (cellulose sulfate and polystyrene sulfonate) against bacterial vaginosis (bv)-associated bacteria. vaginal application of these microbicides not only may reduce the risk of acquisition of human immunodeficiency virus and other sexually transmitted infection-causing organisms but may also decrease the incidence of bv. | 2002 | 12121959 |
| six-year follow-up survey of sexually transmitted diseases in brasilia, the capital of brazil. | the notification of sexually transmitted diseases (std) is a prime component of well-designed public health policy. however, peculiar aspects of std must be taken into account for the correct management of surveillance activities. here, we describe the distribution of the most common sexually transmitted diseases among patients attended by the gynecological clinics of the principal public hospitals of brasilia and the federal district, brazilian capital, during six years. a total of 142,158 pati ... | 2002 | 12144746 |
| correlation of local interleukin-1beta levels with specific iga response against gardnerella vaginalis cytolysin in women with bacterial vaginosis. | mucosal immune system activation may represent a critical determinant of adverse sequelae correlated with bacterial vaginosis, as hiv sexual transmission, upper genital tract infections, cervicitis, endometritis, postsurgical infections, and adverse pregnancy outcomes as preterm delivery (ptd), low birth weight (lbw). | 2002 | 12148539 |
| dna hybridization test: rapid diagnostic tool for excluding bacterial vaginosis in pregnant women with symptoms suggestive of infection. | this prospective comparative study evaluated a dna hybridization test (affirm vpiii) as an alternative to gram stain for the diagnosis of bacterial vaginosis. we examined vaginal smears from 1,725 pregnant women between the 12th and 36th weeks of gestation with clinical signs of vaginal infection. the dna hybridization test compared well with gram stain and can be used as a rapid diagnostic tool to exclude bacterial vaginosis. | 2002 | 12149379 |
| improving gram-stained reproducible result by further adding clue cells in diagnosing bacterial vaginosis. | the reproducibility of interpretation in diagnosing bacterial vaginosis may be enhanced by adding pus cells and clue cells into two different criteria, developed by spiegel et al. and nugent et al. the purpose of study was designed to find out which parameter was more reproducible. 100 patients were collected with the diagnosis of bacterial vaginosis as an experimental group, while the other 100 patients who were with routine papanicolaou smears in gynecologic clinic the collected as a control g ... | 2002 | 12164009 |
| vaginal microbiota in healthy pregnant women and prenatal screening of group b streptococci (gbs). | the microbiota of the lower female genital tract was evaluated from vaginal swabs obtained from 623 healthy pregnant women at gestation periods of 35-40 weeks. isolated and identified microorganisms were expressed as percentages of total samples. as expected, lactobacilli made up the dominant vaginal microbiota (70%). enterobacteriaceae, mainly escherichia coli, klebsiella spp. and proteus, were present in 38% of the samples, which might reflect the possible contamination of vaginal tract with r ... | 2002 | 12180785 |
| [common backache of unusual etiology]. | | 2002 | 12185944 |
| safety and tolerability of the new contraceptive sponge protectaid. | this study was undertaken to assess the efficacy, safety and acceptability of two different-dose regimens of the protectaid contraceptive sponge. | 2002 | 12201327 |
| armenia: reducing morbidity due to stds. | although the incidence of sexually transmitted diseases (stds) and hiv infections in armenia is not as high as that of the other nis republics, the occurrence of syphilis and other stds caused by pathogens such as chlamydia, ureaplasma, and gardnerella has continued to rise over the past 5 years (1994-98). this has led to the implementation of projects that focus on the control of stds, primarily syphilis. one such project is the screening of all women with pelvic inflammatory disease. anothe ... | 1999 | 12222306 |
| anogenital bacteriology in non-abused preschool children: a descriptive study of the aerobic genital flora and the isolation of anogenital gardnerella vaginalis. | the purpose of the study is to describe the genital aerobic bacterial flora including gardnerella vaginalis in girls and the occurrence of anal g. vaginalis in both genders. from a group of 3773 children, 278 (99 boys and 179 girls) with a mean age of 5.63 y (range: 5.13-6.73) were recruited. inclusion in the study was based on self-selection, whereby parents who did not suspect any occurrence of sexual abuse of their child gave informed consent to participate. several mechanisms were undertaken ... | 2002 | 12222710 |
| metronidazole and pregnancy. | | 2002 | 12228952 |
| vaginal carriage of anaerobic motile curved rods: relation to contraceptive practice, common pathogens, signs and symptoms. | the carriers of a species of anaerobic motile curved rods, often seen in cases of bacterial vaginosis, were compared with matched controls with respect to type of contraception, current symptoms and signs, and diagnosis of sexually transmitted disease (std). 65 carriers and 72 controls were ascertained from a std clinic. the anaerobes were demonstrated by phase contrast microscopy in a wet vaginal smear in 17.8% of 366 consecutive admissions. these organisms are difficult to culture, but are ... | 1986 | 12281325 |
| [a comparative study of the incidence of gardnerella vaginalis in users of iud and oral contraceptives]. | leukorrhea constitutes one of the most frequent complaints by women visiting out-patient gynecological clinics. the most common etiological agents are gardnerella vaginalis, trichomonas vaginalis, neisseria gonorrhoea and candida albicans. some authors have been able to verify an increased presence of certain pathogenic germs in the vaginal flora for users of contraceptive methods, e.g., for users of iud, kivijarvi et al. demonstrated a significantly increased presence of gardnerella vaginalis ... | 1987 | 12282423 |
| [vaginitis due to gardnerella vaginalis in a university medical service]. | between august 1983 and june 1984, a total of 363 women were studied who had been referred to the medical service of the school of bacteriology and laboratory clinic of the university of antioquia, medellin, colombia, for obtaining vaginal cytology. they either had or did not have vaginal discharge. the smears were tested for gardnerella vaginalis and trichomonas vaginalis. the majority of the patients were in the third, fourth, and fifth decades of their lives: 51%, 24.5%, and 14%, respectiv ... | 1985 | 12292701 |
| [clinical evaluation of combined use of the spermicide nonoxynol-9 and condoms]. | condoms represent an efficient family planning method. their use was 1st described by the italian anatomist fallopio in 1564. their effectiveness is considered to result in 0.5-2 births/100 years of marriage. 1st descriptions of vaginal contraceptives, by the egyptians, date back to 1850 b.c. their effectiveness is considered to be 5-10/100 years of marriage. a combined usage of condom and spermicides such as nonoxynol-9 could thus elevate contraceptive effectiveness more than 20 times. a ... | 1988 | 12316192 |
| bacterial vaginosis associated with g vaginallis / mobiluncus sp: ultrastructural parameters. | physicians at the national institute of perinatology in mexico city, mexico used a carl-zeiss em 10c electron microscope to examine genital secretion samples from 10 pregnant women (15-38 weeks' gestation) who had been diagnosed with mobiluncus species and gardnerella vaginalis infections to illustrate the form and structure of bacteria responsible for bacterial vaginosis. they were concerned that these bacteria induce preterm labor and premature rupture of membranes (pl/prom). these bacteria ... | 1992 | 12317783 |
| gardnerella vaginalis bacteremia in a premature neonate. | gardnerella vaginalis is a normal component of the human vaginal flora and commonly associated with bacterial vaginosis. invasive infection in obstetrical patients due to g. vaginalis has also been reported. in the pediatric age range, infection due to g. vaginalis is extremely rare and limited to neonates. we describe a 23-week premature infant with g. vaginalis bacteremia and review the characteristics of neonatal g. vaginalis infection reported in the literature. antibiotic susceptibility tes ... | 2002 | 12368979 |
| a multiplex polymerase chain reaction-based diagnostic method for bacterial vaginosis. | to develop a polymerase chain reaction (pcr)-based diagnostic method for bacterial vaginosis using bacterial vaginosis-associated anaerobes. | 2002 | 12383546 |
| douching in relation to bacterial vaginosis, lactobacilli, and facultative bacteria in the vagina. | to study how frequency, recentness, and reason for douching impact bacterial vaginosis-related vaginal microflora and the occurrence of cervical pathogens. douching has been linked to bacterial vaginosis as well as to chlamydial cervicitis in some, but not all, studies. | 2002 | 12383547 |
| [sexually transmitted infections]. | sexually transmitted diseases (std) are quite common and observed more frequently in teens. the adolescents represent a group at particular risk for std due to biological, sociocultural and psychological factors. undectected infections may lead to unwanted sequelae, including pelvic inflammatory disease, chronic abdominal pain, tubal scarring and increased risk of ectopic pregnancy. this paper deals with infections by candida albicans, chlamidia tracomatis, neisseria gonorrheae, gardnerella vagi ... | 2002 | 12388942 |
| vaginal hydrolytic enzymes, immunoglobulin a against gardnerella vaginalis toxin, and risk of early preterm birth among women in preterm labor with bacterial vaginosis or intermediate flora. | the purpose of this study was to determine whether the microbial hydrolytic enzymes, sialidase and prolidase, and immunoglobulin a against the gardnerella vaginalis cytolysin (anti-gvh iga) increase the risk for early preterm birth (< or =34 weeks of gestation) among women with bacterial vaginosis or intermediate flora. | 2002 | 12388968 |
| characterization and selection of vaginal lactobacillus strains for the preparation of vaginal tablets. | to characterize and select lactobacillus strains for properties that would make them a good alternative to the use of antibiotics to treat human vaginal infections. | 2002 | 12392537 |
| antimicrobial activity of dequalinium chloride against leading germs of vaginal infections. | dequalinium chloride (cas 522-51-0) and povidone iodine (cas 25655-41-8) are known as antiseptic agents and used in the local treatment of vaginal infections. clotrimazole (cas 23593-75-1) is an anti-fungal drug and applied primarily in the therapy of vulvo-vaginal candidiasis and to a lesser extent in bacterial vaginosis and trichomoniasis. however, antimicrobial activities of those three agents in comparison to each other have not been reported so far. to address this issue the antimicrobial a ... | 2002 | 12404886 |
| reactive arthritis induced by gardnerella vaginalis. | | 2002 | 12412212 |
| [microbiological investigation of the vaginal flora during pregnancy]. | the infections complications during pregnancy and delivery are jet unsolved problem in obstetrics and neonatology. most of them are due to anaerobic and aerobic organisms. the authors show the rate and distribution of the bacterial vaginal infections during pregnancy. 1330 pregnant women are investigated, 10% of them are with normal vaginal flora, 27% anaerobic organisms, 15% aerobic organisms, then candida albicans and gardnerella vaginalis. those results are seen most frequently during the las ... | 2002 | 12412347 |
| in vitro activities of garenoxacin (bms 284756) against 108 clinical isolates of gardnerella vaginalis. | garenoxacin (bms 284756) was active against 105 of 108 (97%) recent clinical gardnerella vaginalis isolates at < or =2 micro g/ml by using the reference agar dilution method for anaerobes. twenty-eight percent of isolates (31 of 108) were resistant to metronidazole, and 44% were resistant to doxycycline. all were susceptible to clindamycin and ampicillin-sulbactam. | 2002 | 12435709 |
| chronic intrauterine and fetal infection with gardnerella vaginalis. | we sought to develop a model of chronic intrauterine and fetal infection with gardnerella vaginalis. | 2002 | 12439517 |
| detection of bacterial vaginosis-related organisms by real-time pcr for lactobacilli, gardnerella vaginalis and mycoplasma hominis. | the aim of this study was to determine the feasibility of detecting bacterial vaginosis (bv)-related organisms in stored genital tract specimens using real-time pcr. frozen cervicovaginal lavage (cvl) samples from 21 women were analyzed by real-time pcr for the numbers of mycoplasma hominis, gardnerella vaginalis and lactobacilli. lactobacilli organisms were detected in all cvl samples, g. vaginalis was detected in all but one sample, while m. hominis was detected in only six samples. using the ... | 2002 | 12443827 |
| [modern methods for the evaluation of qualitative and quantitative changes in the characteristics of intestinal and vaginal microflora]. | disturbances in normal intestinal and vaginal microflora in women have recently become quite frequent. this accounts for the need of bacteriological laboratories for introduction of reliable methods for the diagnosis of such disturbances. correct methodological approaches to objective evaluation of the state of intestinal and vaginal microflora are described. the methods used in the study of anaerobic microflora (lacto- and bifidobacteria, eubacteria, peptostreptococci, clostridia, bacteriods, f ... | 2002 | 12449705 |
| microbiology and management of polymicrobial female genital tract infections in adolescents. | this review describes the microbiology and management of female genital tract infections in adolescents. these infections include vulvovaginitis, vulvovaginal pyogenic infections (abscesses of bartholin's and skene's glands, infected labial inclusion cysts, labial abscesses, furunculosis, and hidradenitis), endometritis, pyometritis, salpingitis, pelvic inflammatory disease, and tubo-ovarian and pelvic abscess. anaerobes can be cultured in 50% to 90% of females with a variety of genital infectio ... | 2002 | 12459228 |
| effectiveness of current therapy of bacterial vaginosis. | the study was conducted in order to evaluate effectiveness of the treatment of bacterial vaginosis (bv) with different therapeutic regimes according to recommendations of the world health organization (who). during a one-year period (february 2000-february 2001) the sexually transmitted diseases (std) center was visited by 482 women aged 14-51. the diagnosis of bv was established by standard methods: amsel's clinical criteria and gram stain of vaginal discharge. the first-line treatment was oral ... | 2002 | 12467186 |
| [retrospective microbiologic evaluation of vaginal cultures]. | vaginal culture is one of the most difficult cultures to be evaluated in clinical microbiology practice. the necessity of some expensive and complicated processes for diagnosis of some specific agents, age related variability of normal vaginal flora and failure caused by temporary presence of some pathogens in normal flora can be listed among the probable causes of that problem. in this study 8050 vaginal cultures performed in our hospital laboratories between 1 march 1999-15 september 2001 were ... | 2002 | 12476763 |
| recurrent bacterial vaginosis in a virgin adolescent: a new method of treatment. | bacterial vaginosis (bv) is a polymicrobial infection of the vagina and should not be considered an exclusively sexually transmitted disease. we describe the case of a 17-year-old female virgin adolescent with recurrent malodorous vaginal discharge for 6 months. before referral to us she had been treated unsuccessfully with conservative treatment options. our investigation revealed gardnerella vaginalis as the responsible factor for the vaginal infection. because metronidazole treatment had fail ... | 2002 | 12478334 |
| [allelic polymorphism of the tem(m) determinant in mycoplasma hominis and ureaplasma urealyticum clinical isolates resistant to tetracyclines]. | of the 130 clinical isolates of mycoplasma hominis from patients with nonspecific inflammatory diseases of the urogenital tract (ugt), approximately 10% contained the tet(m) gene after the course of treatment with tetracyclines. this gene was found in nine (25%) of the 36 ureaplasma urealyticum clinical isolates. the nucleotide sequence of 13 tet(m) genes in tcr clinical isolates of m. hominis and five genes in u. urealyticum tcr clinical isolates was determined. a comparison of nucleotide seque ... | 2002 | 12500671 |
| determination of immunoglobulin a against gardnerella vaginalis hemolysin, sialidase, and prolidase activities in vaginal fluid: implications for adverse pregnancy outcomes. | a nested case-control study of low birth weight and preterm delivery was performed with singleton women. immunoglobulin a (iga) against the gardnerella vaginalis hemolysin (anti-gvh iga) and sialidase and prolidase activities were determined in vaginal fluid at 17 weeks of gestation. sialidase positivity and bacterial vaginosis with high prolidase activity were associated with 2- and 11-fold increased risks for low birth weight, respectively. no woman with bacterial vaginosis plus a strong anti- ... | 2003 | 12517887 |
| [sexual transmissible diseases and "returning leukorrhea"]. | white discharges and sexual transmissive diseases are now increasing. confronted to this symptom, the general practician can easily diagnose and treat his patient. therefore he must keep in his mind that behind this common symptom, a much more serious disease can be hidden. | 2002 | 12522941 |
| [amplification of bacterial heat shock protein 60 gene using inverse pcr method]. | a method is presented for rapid in vitro amplification of dna sequences of bacterial hsp60 gene. in our previous work, using degenerate oligonucleotide primers for conserved regions of hsp60 gene, a 600 bp fragment was amplified by pcr, cloned and sequenced. an inverse pcr method, with the primers oriented in the reversed direction of the usual orientation, is used to amplify the dna sequences that flank the 600 bp known region. the feasibility of this method is shown by amplifying the complete ... | 2002 | 12557349 |
| [role of bacteria associated with sexually transmitted infections in the etiology of lower urinary tract infection in primary care]. | urinary tract infections (uti) are the second most frequent type of infectious pathology treated in primary care clinics. the participation of microorganisms associated with sexually transmitted infection has been reported as a cause of uti; nevertheless this concept is still controversial. to gather data on this subject, we carried out a search for gardnerella vaginalis, ureaplasma urealyticum, mycoplasma hominis and streptococcus agalactiae besides the common microorganisms involved in uti. | 2003 | 12586032 |
| role of the vaginal microbiological ecosystem and cytokine profile in the promotion of cervical dysplasia: a case-control study. | to identify alterations in the cytokine profile and microbial ecosystem of the vagina in association with cervical dysplasia. | 2002 | 12648311 |
| disseminated gonococcemia. | a 26-year-old woman presented with a high-grade fever and chills of 2 days' duration. she complained of associated joint pain, especially in the wrists and knees. one day before admission, tender skin lesions began to develop on the fingers, and subsequently spread to the more proximal extremities. the patient recalled having a sore throat and a nonproductive cough before the onset of the fever and eruption. the past medical history was significant for gardnerella vaginitis and several urinary t ... | 2003 | 12653917 |
| detection of bifidobacterium species and gardnerella vaginalis in the vagina using pcr and denaturing gradient gel electrophoresis (dgge). | | 2003 | 12676400 |
| increased risk of cervical canal infections with intracervical foley catheter. | to evaluate the effect of intracervical foley catheter insertion, for the induction of labor, on cervical canal infection. | 2003 | 12689532 |
| activity of novispirin g-10, a novel antimicrobial peptide against chlamydia trachomatis and vaginosis-associated bacteria. | we tested the activity of novispirin g-10, a novel antimicrobial alpha-helical octadecapeptide structurally related to cathelicidins and other innate immunity peptides, against chlamydia trachomatis serovars l2, d, and e and three organisms associated with bacterial vaginosis (bv). the peptide's activity against c. trachomatis was measured in 48-h shell vial assays with mccoy cell targets. exposure to 100 micro g/ml of novispirin g-10 reduced the infectivity of serovars d and e by 99.4-100% and ... | 2003 | 12710952 |
| laboratory diagnosis of gardnerella vaginalis vaginosis. | an evaluation of various laboratory detection methods and characteristics of gardnerella vaginalis was made using high vaginal swab samples of 470 out patient clinic--attending women. gram stain for 'clue cells' showed positive results in 118 (25.1%)cases; culture, in 100 cases, (213%) and amine odour (213%), in 26 cases 5.5%, majority, 71 cases, of the culture-positive results were associated with a ph value of 6 to 7. gardnerella vaginalis grew predominantly in enriched culture media: modified ... | 2002 | 12744579 |