| response of congenitally athymic (nude) mice to infection with mycobacterium bovis (strain bcg). | mice homozygous for the mutation "nude" (nu/nu) are athymic and lack thymus-dependent lymphocytes. heterozygote (nu/+) controls, which are phenotypically and immunologically normal, and (nu/nu) mice were infected with 1.0 times 10-6 colony-forming units of phipps strain bcg. in the spleens of nu/+ mice, there was a progressive increase in number of bcg up to the second week, followed by a gradual decline. however, in the nu/nu mice, bcg growth was gradual and continuous until termination of the ... | 1975 | 1094126 |
| hl-a antibody response in patients with acute myelogenous leukaemia treated by immunotherapy. | | 1975 | 1094639 |
| microcytotoxicity and serum blocking factors in malignant melanoma and halo nevus. | microcytotoxicity assays of patients with malignant melanoma and halo nevi were performed. no good correlation could be found between percent cell inhibition and histopathological level of melanoma or the clinical staging. the percent cell inhibition was usually an index of response to vaccinia virus immunotherapy. actively regressing halo nevi showed high levels of percent cell inhibition, whereas inactive halo nevi had low levels of percent cell inhibition and blocking factor. immunologic reac ... | 1975 | 1094959 |
| proceedings: in vivo mechanisms of bcg immunosuppression. | | 1975 | 1095023 |
| [study of unheated filtrates of cultures of mycobacteria. ii. isolation of catalase of the filtrate of cultures of bcg-poland bacilli and its biological activity]. | | 1975 | 1095450 |
| [study of unheated filtrates of cultures of mycobacteria. iii. secies specificity of dialysable components of m. bovis (bcg-poland) filtrates and m. kansasii]. | | 1975 | 1095451 |
| in vitro lymphocyte response to purified protein derivative, bcg and mycobacterium leprae in a population not exposed to leprosy. | lymphocytes from 14 bcg-vaccinated donors, seven tuberculin positive and seven tuberculin negative by skin testing, were stimulated in vitro with four mycobacterial antigens, purified protein derivative (ppd), ppd/bcg, whole bcg bacilli, and whole mycobacterium leprae and also with candida antigen and phytohemagglutinin. the response was measured by incorporation of (3)h-labeled thymidine. the response to ppd, ppd/bcg, and bcg was found to correlate with the result of skin testing with turbercul ... | 1975 | 1095479 |
| bcg suppresses growth and metastasis of hydatid infections. | | 1975 | 1095933 |
| [bactericidal action of disinfectants on some strains of acid-fast bacilli. ii. surface-active compounds]. | | 1975 | 1096245 |
| cultures of pig macrophages. | freshly collected blood from pigs was defibrinated with glass beads. the white cells were collected on a ficoll-hypaque mixture and grown in rpmi 1640 medium containing 20 per cent fetal calf serum. monocytes adhered to the plastic or glass flasks and the erythrocytes and lymphocytes remained in suspension. the adherent cells assumed the appearance of macrophages and the mean cell size at eight days was 40 mum. phagocytosis was demonstrated and, in the first few days, incorporation of tritiated ... | 1975 | 1096254 |
| bcg inhibition of melanoma: specific? | | 1975 | 1096302 |
| [tuberculosis in mink]. | | 1975 | 1096453 |
| multiple mycobacterial antigens in diagnostic tuberculins. | guinea-pig antisera containing igg1 antibodies have been prepared either with killed mycobacterial in paraffin oil or living bcg or intradermal injections of fractions of unheated culture filtrate. sera, found to contain antibodies specific for different mycobacterial antigens are used in passive cutaneous anaphylaxis (pca) in 12 times the dilution-to-extinction titer. animals prepared with sera respond in all sites when unheated culture filtrate, strain h37rv, is injected intravenously (i.v.) a ... | 1975 | 1097280 |
| physical-chemical properties of precipitated and fractionated allergens from mycobacterium bovis. | seven allergens were prepared from cultures of mycobacterium bovis. the culture filtrates were divided in half and each aliquot precipitated with either ammonium sulfate or trichloroacetic acid to prepare purified protein derivatives (ppd). these samples of ppd-bovis were analyzed for nitrogen, carbohydrate, rna and dna. physical tests, such as: cellulose acetate electrophoresis, disc gel electrophoresis, ultracentrifugation, isoelectricfocusing and gel filtration were also performed. precipitat ... | 1975 | 1097281 |
| passive transfer of unresponsiveness by lymph node cells. studies on adjuvant disease. | pretreatment of rats with subarthritogenic doses of mycobacterial adjuvant suppresses the arthritogenic reaction induced by this adjuvant. this unresponsiveness can betransferred to normal syngeneic recipients by viable lymph node cells from unresponsivedonors. serum from the same donors did not prevent the arthritis. abrogation ofunresponsiveness was obtained by injecting normal lymph node cells together with the challeging inoculation and also with sensitized lymphoid cells. in agreement w ... | 1975 | 1097332 |
| qualitative and quantitative thin-layer chromatography of mycolic acids in mycobacterium tuberculosis var. bovis-bcg. | | 1975 | 1097459 |
| cutaneous infection due to a rough variant of mycobacterium marinum. | spreading lesions clinically resembling lymphangitic sporotrichosis developed on the right arm and chest of a 60-year-old man with chronic lymphocytic leukemia. acid-fast bacilli were seen in exudates from lesions and in biopsies, and were cultured from them. the isolant grew initially as a yellowish-orange scotochromogen on lowenstein-jensen medium at room temperature and at 35 c., but failed to grow at 37 c. it failed to grow on 7-h-10 medium. on repeated subculturing over a 2-year period it g ... | 1975 | 1098441 |
| development of specific lymphocyte immunostimulation and tuberculin skin reactivity in swine infected with mycobacterium bovis and mycobacterium avium. | swine infected with mycobacterium bovis and mycobacterium avium were studied for development and specificity of lymphocyte immunostimulation and tuberculin skin reactivity to purified protein derivatives (ppd) of tuberculins. lymphocytes from infected animals had maximal blastogenic responses to ppd prepared from the homologous organisms. the time of appearance of specifically sensitized lymphocytes was studied, as was the specificity of skin reactivity. in vitro lymphocyte responses of m avium ... | 1975 | 1098528 |
| [the influence of mycobacterium bovis bcg-prague, respectively of mycobacterium smegmatis on the growth of the intratracheally transplanted deal's sarcoma in guinea pigs (author's transl)]. | | 1975 | 1098744 |
| lymphokines. i. use of insoluble concanavalin a for the production of migration inhibitory factor in guinea pig lymphocyte cultures. | incubation of guinea pig lymph node lymphocytes with insoluble concanavalin a (con a) in serum-free medium yields sizable amounts of migration inhibition (mif) and mitogenic factors (mf). various controls confirmed that the activities detected in supernatants from such cultures are not due to free soluble con a released during culture. whereas guinea pig mif does not act upon human and horse monocytes, guinea pig mf stimulated human but not horse lymphocytes. upon stimulation by insoluble con a, ... | 1975 | 1099010 |
| an animal model of hypersensitivity pneumonitis in the rabbit. | this study was devised to produce an animal model of hypersensitivity pneumonitis in order to study both the induction and the elicitation of the disease. rabbits exposed by aerosol to large quantities of pigeon antigens developed a humoral, but not cellular, immunologic response. moreover, their lungs were essentially normal histologically. a single i.v. injection of killed bcg in oil permitted the induction of pulmonary cell-medid hypersensitivity to the inhaled antigen, as well as the develop ... | 1975 | 1099122 |
| [the characteristics of the antibacterial activity of garlic (author's transl)]. | it has been found by cavallito (1944) that garlic (allium sativum l.) has a strong antibacterial activity and the active principle of garlic is named "allicin" and it has been confirmed by stoll et al. (1948) that the allicin is derived from the alliin-alliinase system. nevertheless, one worker in japan (1951) stands on another view point which the effective component of garlic is some sugar-proteim because he could not find alliin in the native garlic. we intend to make sure that the alliin is ... | 1975 | 1099271 |
| therapeutic effect of a water-salt extract from bcg in two patients with advanced breast cancer. | two cases of advanced breast cancer treated with a water-salt extract from bcg are presented, showing a positive response -- marked and durable regression of the breast tumor and of the axillar metastases, parallel in the second case with a disappearance of lung metastases. | 1975 | 1099468 |
| nonspecific resistance to tumor allograft. | the effects of an increased volume of reticulohistiocyte system and macrophage activation in the recipient after treatment with bcg and zymosan on the course of antitumor concomitant allograft-immunity reaction were studied. the results were verified on animals affected with immunosuppression due to whole body irradiation. a factor of essential significance for resistance to tumor allograft is an increase of the total number of small lymphocytes supplied by the transplantation of syngeneic splee ... | 1975 | 1099469 |
| isolation and identification of mycobacteria from porcine tissues: a three-year summary. | mycobacteria were isolated from 1,591 (78%) of 2,036 porcine tissues submitted to veterinary services laboratories over a 3-year period (july 1, 1971, to june 30, 1974). the isolates were identified by biochemical and serologic tests. of the 1,547 mycobacterium avium isolates, 452 were serotype 1, 728 were serotype 2, 60 were serotyped 4, 110 were serotype 8, and 51 were serotyped 10; 36 isolates represented 11 other serotypes; 65 isolates shared antigens with more than one serotype; and 45 isol ... | 1975 | 1099946 |
| physiologic and pharmacologic alterations of rat leukocyte chemotaxis (cx) in vivo. | the method of adoptively transferring 51cr-labeled rat leukocytes iv to isologous recipients was used to quantitate the extravascular accumulation of specific cell types at the site of inflammation induced by local injection of various phlogistic agents. experiments were designed to determine whether cellular accumulation could be modified at the level of the chemotactic factor, by serum components, or by alteration of the responding cell itself. the results indicated a selective attraction of m ... | 1975 | 1099959 |
| a preliminary report on the effects of methanol extraction residue of bcg (mer) on cancer patients. | twenty-seven patients with malignant neoplasia were injected intradermally with the methanol extraction residue (mer) fraction of tubercle bacilli. two schedules of treatment were used: every other week and once a month; 1-10 courses of mer were administered to the patients. the skin reactivity to 3 recall antigens, as well as to the injected mer itself, was used to monitor the immune response. improvement of skin reactivity occurred in 9 of 18 patients tested with recall antigens. five of 6 pat ... | 1975 | 1100088 |
| phospholipid metabolism of stimulated lymphocytes. composition of phospholipid fatty acids. | lymph node lymphocytes and thymocytes from different species were isolated. rabbit and calf thymocytes were stimulated in vitro with concanavalin a. phospholipid fatty acids of these cells were analyzed and their positional distribution was determined. when compared with liver, phosphatidylcholine of unstimulated lymphocytes was found to contain relatively high amounts of palmitic acid in position 2 and oleic acid in position 1. after stimulation of rabbit thymus cells, the content of polyunsatu ... | 1975 | 1100108 |
| the influence of mycobacterium bovis bcg-prague, respectively of mycobacterium smegmatis on the growth of the intratracheally transplanted deal's sarcoma in guinea pigs (author's transl)]. | | 1975 | 1100201 |
| effects of migration inhibiting factor(s) on the in vitro detachment of macrophages. | supernatants from spleen cells, derived from mice injected with bcg and cultured with ppd, specificially inhibited the migration of normal mouse peritoneal macrophages when compared with control supernatants. migration inhibitory supernatants were also shown to decrease the detachment of adherent macrophages in a novel test system. both macrophage migration and detachment inhibitory activities were abrogated by neuraminidase and chymotrypsin treatment of supernatants but were thermostable, sugge ... | 1975 | 1100729 |
| mechanisms by which tumors avoid destruction by the immune system bcg-catalyzed increase in igg and iga blocking activity of lymphocyte-mediated cytotoxicity. | the immunoglobulin igm fraction from the serum of one week sarcoma-bearing balb/c mice increased, the igg and iga fractions from the same animal had no effect on the lymphocytemediated cytotoxic release of 51cr from 51cr-labelled sarcoma cells. these latter two immunoglobulin fractions from serum of 14 days, or more, sarcoma-bearing mice inhibited the lymphocyte-mediated cytotoxic activity. the iga, igg or igm fractions from mice inoculated with bacillus calmette-guerin (bcg) had no effect, but ... | 1975 | 1100900 |
| peripheral nervous system involvement in experimental tuberculosis. | following intravenous injection of mycobacteria in the mouse the bacilli are regularly found in the peripheral nervous system after the 5th day. the peripheral nerves and autonomic ganglia are involved at the level of the perineural cells and the sheath of schwann. when the organisms are of low virulence (bcg, mycobacterium intracellulare) the development of the nodules is limited. in the case of a virulent strain (ravenel) extensive granulomas are found but epitheloid cells, always found in oth ... | 1975 | 1101166 |
| clinical studies of methanol extraction residue fraction of bacillus calmette-guérin as an immunostimulant in patients with advanced cancer. | forty patients with advanced gastrointestinal cancer, all previous chemotherapy failures, were treated with a methanol extraction residue of bacillus calmette-guérin (mer) alone, administered intradermally in either weekly or every-4-week schedules. the only significant side effect was local cutaneous reaction ranging from papules through pustules to draining ulcerations. on the weekly schedule these reactions frequently reached a point of clinical intolerability. in spite of the advanced nature ... | 1975 | 1102082 |
| immunotherapy of human malignant melanoma with oil-attached bcg cell-wall skeleton. | a first case of malignant melanoma treated with oil-attached bcg cell-wall skeleton (bcg-cws) is reported. the patient, a 70-year-old farmer, with metastatic malignant melanoma was treated intralesionally with oil-attached bcg-cws or was given intradermally with oil-attached bcg-cws together with irradiated autologous and allogeneic tumor cells. four months after the start of the immunotherapy with oil-attached bcg-cws, primary tumor and metastatic inguinal lymph nodes regressed markedly without ... | 1975 | 1102379 |
| effect of nocardia and mycobacterium cell-wall skeleton on autochthonous tumor grafts. | cell-wall skeleton of nocardia rubra and of mycobacterium bovis bcg in the oil-attached form, when injected mixes with autografts of spontaneous mammary adenocarcinoma or of methylcholanthrene-induced sarcoma of mice, suppresses the growth of tumor autografts to a demonstrate extent. the bcg whole cell wall showed no effect. the local destruction of tumor autografts did not induce recognizable systemic immunity to the respective tumors. these findings are commended upon from immunological and ch ... | 1975 | 1102381 |
| letter: use of bcg in place of tuberculin to identify human tuberculous infection. | | 1975 | 1102451 |
| [non-tuberculous infections caused by mycobacteria]. | | 1975 | 1102477 |
| effect of freeze-drying on the colony morphology of various daughter strains of bcg. | | 1975 | 1102547 |
| the l.a.i. microtest: a rapid and sensitive precedure for the demonstration of cell-mediated immunity in vitro. | a new microtest for cell-mediated immunity is described, which is based on the elaboration of a diffusible factor from sensitized lymphocytes following contact with specific antigen. the factor inhibits the adherence of neighbouring leucocytes to plastic surfaces. production of the factor is ablated by pretreatment of lymphocytes with theta-antiserum. it is suggested that the factor may be a new lymphokine, leucocyte adherence inhibition factor (laif). | 1975 | 1102612 |
| studies on the respiratory and systemic cell-mediated immunity. development of local and systemic cell-mediated immunity in guinea pigs given killed bcg intratracheally or parenterally. | | 1975 | 1102748 |
| [defense mechanisms against tuberculosis infection in the immune host. the interrelation between activated macrophages and 14c-labeled bcg in primary bcg lesions and those of reinfection (author's transl)]. | | 1975 | 1102749 |
| general immune status in cancer therapy. | theoretical considerations in immunotherapy, such as the timing of challenge, which could rank second to reduction of tumour volume in producing lasting regressions, are outlined and illustrate the need for tests to monitor immune status. estimation of immunosuppression, in order to define cancer therapy schedules providing least interference with what might be useful host response to tumour, is described in terms of a reliable radioimmunoassay method assessing lymphocyte replicating ability (lr ... | 1975 | 1102884 |
| an endotoxin-induced serum factor that causes necrosis of tumors. | in studying "hemorrhagic necrosis" of tumors produced by endotoxin, it was found that the serum of bacillus calmette--guerin (bcg)-infected mice treated with endotoxin contains a substance (tumor necrosis factor; tnf) which mimics the tumor necrotic action of endotoxin itself. tnf-positive serum is as effective as endotoxin itself in causing necrosis of the sarcoma meth a and other transplanted tumors. a variety of tests indicate that tnf is not residual endotoxin, but a factor released from hos ... | 1975 | 1103152 |
| proceedings: transmission of tubercle bacilli: with particular reference to mycobacterium bovis. | | 1975 | 1103396 |
| study of local and systemic effects of intravesical bcg. | defects in cell-mediated immunity have been implicated as one of the underlying causes for the appearance and progression of neoplasms. one approach toward correcting these defects employs immune potentiators for the purpose of stimulating cell-mediated immunity. bcg is the immune potentiator which has been used most frequently in the experimental and clinical situation. a preliminary study directed toward ascertaining the local histologic changes and systemic serum response to bcg injection in ... | 1975 | 1103421 |
| [theoretical principles of clinical tumor immunology]. | | 1975 | 1103468 |
| the effect of a tubercle lipid adjuvant on the distribution of injected foreign red blood cells. | increased localization occured of injected foreign red cells in the spleen and lungs of animals treated with a tubercle bacillary lipid adjuvant given intravenously. the distribution changes varied depending upon the time interval between injections of the adjuvant and the foreign red cells. these changes offer an explanation of the augmentation of haemolysins and haemagglutinins previously shown for the lipid. | 1975 | 1103932 |
| factors affecting the immunizing activity of ribosomal fractions isolated from mycobacterium tuberculosis var. bovis strain bcg. | levels of antituberculous immunity similar to those induced by live bcg vaccine were detected in cf1 mice immunized with ribosomal fractions isolated from mycobacterium tuberculosis var. bovis, strain bcg, and challenged 3 weeks later with the virulent h37rv strain of mycobacterium tuberculosis var. hominis. the activity of the crude ribosomal preparations was found to be a function of the immunizing doses and the immunity induced by 1.0-mg doses remained at the same high level after 4 weeks of ... | 1975 | 1104114 |
| editorial: universal presence of antibodies to microorganisms in sera from normal persons. | | 1975 | 1104283 |
| bcg in cancer and leukemia treatment and prophylaxis-a review. | five international oncology meetings crystallized the concept that the immune system plays a dominant role in the prevention and inhibition of neoplasia; that the cancer antigens are relatively weak, therefore eliminating weak or uneffective cellular immunity which is paramount in tumor rejection; that humoral antibodies against neoplastic antigens may block the cytotoxic action of t cells against the tumor cells. a number of agents have been found capable of stimulating the immune system (t-cel ... | 1975 | 1104732 |
| a technique for intensifying bcg sensitivity. | sensitization to bcg (bacille calmette-guérin) appears to be more effective when it is carried out at the site of a delayed hypersensitivity challenge reaction to dncb (1-chloro, 2,4-dinitrobenzene) than when it is carried out at unchallenged sites in non-sensitized animals. the effectiveness of bcg sensitization was determined by measurement of the intensity of the ppd (purified protein derivative of tuberculin) 24-hour skin test. possible mechanisms for this phenomenon and its implications for ... | 1975 | 1104733 |
| relation between enhanced antibody responses elicited by adjuvant injection and those elicited by secondary antigenic stimulation. | an attempt was made to determine if there is any common mechanism in the enhanced antibody response caused either by injection of adjuvant, such as bacterial endotoxin (lps) and complexed poly-nucleotides, or by secondary antigenic stimulation. lps inoculated in mice 4 days before injection of sheep red cells (srbc) and polya:u invalidated the adjuvant effect of polya:u injected together with srbc, and the hemolysin plaque-forming cell (pfc) response of such mice was similar to that of the mice ... | 1975 | 1104935 |
| nonspecific immunotherapy of malignant tumors. | at present, nonspecific immunotherapy of malignant tumors seems to be the most promising among immunotherapeutic modalities. potent nonspecific immunostimulants, bacillus calmette-guerin (bcg) and corynebacterium parvum, exhibit an antitumor activity in experimental animals, which is commonly manifested by reduced tumor growth and sometimes by complete regression of tumors. antitumor effectiveness of these bacteria is largely related to tumor immunogenicity and host immunocompetence. recently, b ... | 1976 | 1105663 |
| oral bacille calmette guérin immunostimulation in malignant melanoma. | forty-seven patients representing all stages of malignant melanoma have been treated orally with bacille calmette guérin as an adjunct to standard treatment regimens. this is a preliminary study, but it appears that orally administered bacille calmette guérin in localized disease may delay the development of local recurrence and distant spread. in patients with disseminated melanoma but without intracranial metastases, the survival time is increased from 4.0 to 12.7 months. it has no effect on t ... | 1975 | 1105837 |
| [immunologic reactions in malignant melanoma of the skin in man]. | | 1975 | 1105967 |
| the immunological consequences of antigen overload in experimental mycobacterial infections of mice. | mice infected in the tail with m. ulcerans developed transient cell-mediated immunity which disappeared as the bacterial load increased. lymph node cells from animals in this late phase of the disease transformed spontaneously in vitro. this transformation was inhibited by mycobacterial antigen. lymph node cells from mice injected intravenously with 10(8) or 10(9) bcg also transformed spontaneously in vitro. such animals did not become foot pad test-positive. evidence is presented that the spont ... | 1975 | 1106912 |
| extracellular iron acquisition by mycobacteria: role of the exochelins and evidence against the participation of mycobactin. | mycobacterium bovis var. bcg was grown under iron-deficient conditions in the presence and absence of 1% tween 80. mycobactin, the iron iron ionophore of mycobacteria, was found solely within the bacteria grown in the absence of tween, but low concentrations (0.75 mug/ml) of it appeared in the medium in the presence of the surfactant. both types of medium contain agents, named exochelins, which could solubilize iron. 55fe added to spent culture media was recovered only chelated to these compound ... | 1975 | 1107222 |
| effects of bcg, corynebacterium parvum, and methanol-extration residue in the reduction of mortality from staphylococcus aureus and candida albicans infections in immunosuppressed mice. | an immunosuppressed mouse model was devised to test the effects of immunopotentiators on the prevention of bacterial and fungal infections. the effects of bcg and corynebacterium were tested against staphylococcus aureus and candida albicans infection. the effect of methanol-extraction residue (mer-bcg) was tested against s. aureus septicemia. cdf mice were given various doses of bcg, 1.0 mg of c. parvum, or 0.5 mg of mer intraperitoneally at varying intervals before injection of an intravenous ... | 1975 | 1107224 |
| biochemistry of bcg lipids and their role in antituberculous immunity and hypersensitivity. | | 1975 | 1107417 |
| the ultrastructure of the strain bcg 725 cultivated on sauton's medium with casein hydrolysate. co-report the report "biochemistry of bcg lipids and their role in antituberculous immunity and hypersensitivity. | | 1975 | 1107418 |
| thin-layer chromatography of mycolic acids and its application for the characterization of bcg strains. | | 1975 | 1107419 |
| comparative studies with danish and prague bcg strains inoculated into the cornea of the rabbits in repeated experiments. | | 1975 | 1107420 |
| studies of corneal lesions of rabbits inoculated with m. microti--m.p. strain. | | 1975 | 1107421 |
| stimulation of lymphoid cells by components of bcg. | bcg was fractionated into a delipidated mycobacterial cell fraction (dmc) and lipid by exhaustive chloroform-methanol extraction. the effects of these fractions were tested on mouse spleen cells, nonadherent spleen cells (lymphocytes), thymus cells, and adherent spleen cells (macrophages) in vitro and were compared with effects of the whole bacilli and a methanol-extraction residue (mer). tritiated thymidine incorporation into spleen cells, purified spleen lymphocytes, and thymus cells was measu ... | 1975 | 1107573 |
| eradication of microscopic lymph nodes metastases after injection of living bcg adjacent to the primary tumor. | guinea pigs with established intradermal tumors and microscopic axillary lymph node metastases were treated with a combination of surgery and bcg. the tumors were excised and bcg was given in attempts to eliminate residual malignant disease. injection of bcg into established intradermal tumors 7 days before local excision successfully eradicated microscopic axillary lymph node metastases and cured significant numbers of animals. injection of bcg into dermal tumors 20 minutes or 1 day before exci ... | 1975 | 1107574 |
| [bcg-septicemia and mucocutaneous candidiasis due to familial combined immunodeficiency (authors transl)]. | the history of a patient with mucocutaneous candidiasis and bcg-septicemia is reported. combined immunodeficiency has been diagnosed as the underlying cause. a sister of the patient died at seven months of age under similar circumstances. the importance of the immunological diagnosis in severe bacterial and mycotic infection during early infancy has been emphasized. in such cases vaccinations with life vaccine are strongly contraindicated. | 1976 | 1107821 |
| [characteristics of clinical course of pulmonary tuberculosis caused by mycobacterium bovis]. | | 1975 | 1107995 |
| [bcg in the treatment of malignant diseases]. | | 1976 | 1108154 |
| bcg inhibition of murine leudemia: local suppression and systemic tumor immunity require different doses. | the quantitative relationship between bacillus calmette-guérin (bcg) and tumor cells which are optimal for suppressing the growth of tumor cells in bcg-tumor cell mixtures are detrimental to the development of a sustained, systemic tumor rejection immunity in the lstra murine leukemia. | 1976 | 1108198 |
| immunotherapy with neuraminidase-treated cells and bacillus calmette-guerin. | experiments were designed to determine the effectiveness of active immunotherapy, both specific (neuraminidase-treated cells) and nonspecific [bacillus calmette-guerin (bcg) organisms] in the l1210-bdf1 tumor-host system. tumor burden was minimized with chemotherapy (1,3-bis-(20chloroethyl)-1-nitrosourea) prior to immunotherapy. the effectiveness of immunotherapy was dependent on the amount of drug used to minimize tumor burden. an interval 36 hours between chemotherapy and immunotherapy produce ... | 1976 | 1108258 |
| effect of immunization with attenuated mycobacterium bovis on experimental toxoplasmic retinochoroiditis. | administration of attenuated mycobacterium bovis (bacillus calmette-guérin or bcg) provides nonspecific resistance to a variety of microbial infections and tumors. this is associated with a state of augmented immunologic responsiveness. mustering defenses against intracellular parasites, such as toxoplasma gondii, presents a special problem that can be met only by measures that alter the intracellular environment. our study was designed to evaluate the effect of prior immunization of rabbits wit ... | 1975 | 1119523 |
| efficacy of intralesional bcg therapy in guinea pigs with disseminated tumor. | it has been previously demonstrated that transplanted syngeneic tumors established in the skin of inbred (strain-2) guinea pigs regressed and regional lymph node metastases were eliminated after intralesional injection of viable mycobacterium bovis (bcg). during the course of this reaction there is the development of tumor-specific immunity. this experimental model was further manipulated in order that it would more closely approximate a clinical reality. in the present study an evaluation was m ... | 1975 | 1175128 |
| an epizootic of bovine tuberculosis in georgia. | a tuberculous beef animal was detected aug 29, 1973, by a veterinary medical officer in a georgia slaughter establishment. animal identification and traceback eventually led to a purebred beef herd in decatur county, georgia. investigation and tuberculin testing of 23,000 cattle in 350 herds revealed 11 mycobacterium bovis-infected beef herds and resulted in the complete depopulation of 5 herds. the epizootic was confined to georgia and was eradicated by april, 1975. | 1975 | 1184445 |
| intravenous johnin and tuberculin tests in cattle vaccinated with mycobacterium paratuberculosis cells and subsequently inoculated with mycobacterium bovis. | calves at 30 days of age were vaccinated with a killed whole-cell mycobacterium paratuberculosis vaccine. four months later, these calves were inoculated with mycobacterium bovis. the intravenous tuberculin and johnin tests were applied both before and after inoculation. the results of the hematologic investigation had extremes at both high and low values and were too unsuitable for statistical analysis. the intravenous tuberculin test is considered unsuitable for diagnosis of bovine tuberculosi ... | 1975 | 1200443 |
| transmission of bcg-associated tumor resistance from maternal to newborn guinea pigs. | resistance to line-10 hepatocarcinoma was studied in the newborn offspring of inbred strain-2 sewall wright guinea pigs that had recovered from this tumor after prior administration of subcellular components of mycobacterium bovis strain bcg. these mothers gave birth to 15 offspring, 53% of which resisted repeated challenges with this tumor. offspring of mothers who had been given only bcg components were similarly tested. complete protection was observed in 23.5% of 17 babies and attenuation of ... | 1976 | 1255744 |
| effects of biological response modifiers with different modes of action used separately and together on immune responses in mice with syngeneic tumours. | the effect of a protein-bound polysaccharide (psk) obtained from cultured mycelia of the basidiomycetes coriolus versicolor on activities involved in the host defence mechanism of c57bl/6 mice bearing adenocarcinoma 755 was compared with that of live bacille calmette-guérin (bcg). delayed footpad reaction, the activity of splenic natural killer cells and interferon production induced by concanavalin a in splenic cells of healthy mice were little affected by psk, but in mice bearing tumours psk p ... | 1992 | 1280606 |
| analysis of circulating immune complexes from leprosy patients for mycobacterium leprae antigens. | circulating immune complexes (cics) from 31 leprosy patients (16 tuberculoid, 15 lepromatous) and 12 healthy volunteers, precipitated by 3.5% polyethylene glycol, were individually subjected to sds-page and immunoblotting using a variety of monoclonal and polyclonal antibodies against mycobacterium leprae. a common mycobacterial antigen of an apparent molecular size of 65 kda was identified in cics from about 40% of the patients. no correlation was observed between the positivity for this antige ... | 1992 | 1282143 |
| anti-peripheral nerve antibodies in leprosy patients recognize an epitope shared by the m. leprae 65 kda heat shock protein. | binding of leprosy sera to peripheral nerve from different species (mouse, guinea pig and rabbit) was evaluated by elisa. a majority of sera, whatever the clinical form of leprosy, bind to these antigens. absorption with mycobacterium bovis bcg demonstrated that these antibodies recognize cross-reactive epitopes between peripheral nerve and mycobacteria. in immunoblot analysis, both leprosy patient sera and a monoclonal antibody directed at the 65 kda heat shock protein of m. leprae were shown t ... | 1992 | 1283301 |
| mycobacterial adherence and bcg treatment of superficial bladder cancer. | the importance of adherence of bcg (bacillus calmette guerin) to the bladder wall as an initiator of the processes leading to the bcg-induced antitumor activity is still controversial. a study was initiated addressing this subject by an experimental procedure modulating bcg adherence using pretreatment with pentosan polysulphate (pps), a polysulphated polysaccharide with glycosaminoglycan (gag)-like properties and reported bacterial antiadherence properties to the bladder mucosa. furthermore, pp ... | 1992 | 1284504 |
| human tuberculosis due to mycobacterium bovis: report of 10 cases. | during the period 1986-1990, 10 cases of human disease caused by mycobacterium bovis were diagnosed in our hospital. the incidence, in relation to the cases of disease from mycobacterium tuberculosis diagnosed in the same period, was 0.9%. the patients had an average age of 32 years (range 5-68 years). pulmonary disease was observed in 5 patients (50%), lymphadenitis in 2, pleural effusion in 2 and peritoneal in 1. the most significant of the epidemiological features was that 2 patients were vet ... | 1992 | 1292721 |
| mechanisms of action of bcg in superficial bladder cancer. | | 1992 | 1301570 |
| [mechanisms of clearance of foreign bodies by macrophages]. | macrophages have specific functions related to the sites where they are found. pulmonary alveolar macrophages (pam) may play an important role the clearance of invading and degenerative substances from the alveolar spaces. to clarify the mechanisms of the clearance by macrophages, we studied the role of scavenger receptors (sr) and fc receptors (fcr) of pam. 1) sr: expression and localization of sr in human pam. type i and ii srs were expressed in human pam, and the two srs were confirmed in the ... | 1992 | 1306225 |
| isothermal in vitro amplification of dna by a restriction enzyme/dna polymerase system. | an isothermal in vitro dna amplification method was developed based upon the following sequence of reaction events. restriction enzyme cleavage and subsequent heat denaturation of a dna sample generates two single-stranded target dna fragments (t1 and t2). present in excess are two dna amplification primers (p1 and p2). the 3' end of p1 binds to the 3' end of t1, forming a duplex with 5' overhangs. likewise, p2 binds to t2. the 5' overhangs of p1 and p2 contain a recognition sequence (5'-gttgac- ... | 1992 | 1309614 |
| use of gene probes based on the insertion sequence is986 to differentiate between bcg vaccine strains. | gene probes derived from the insertion sequence is986, which have previously been shown to differentiate isolates of mycobacterium tuberculosis for epidemiological analysis, are also capable of distinguishing two groups of bcg vaccine strains. most bcg strains have a single copy of is986, at the same chromosomal site, while the brazilian, japanese and ussr strains have an additional copy at a different, common location. these results correlate with the results of previous antigenic analysis and ... | 1992 | 1313411 |
| testicular pseudotumor caused by mycobacterium bovis epididymitis. | | 1992 | 1313835 |
| characterization of acth mediated suppression of mhc class ii expression by murine peritoneal macrophages. | the effect of acth on the expression of major histocompatibility complex (mhc) class ii (i-a) glycoprotein by murine peritoneal macrophages was evaluated. acth suppressed the expression of i-a by macrophages in a time- and dose-dependent manner. acth mediated its effect by decreasing the level of i-a mrna. acth suppressed the expression of i-a by macrophages from mice that are susceptible to the in vivo growth of mycobacteria but did not affect the expression of i-a by macrophages from mycobacte ... | 1992 | 1320056 |
| in vivo effects of anti-leprosy drugs on the rat peritoneal macrophages and lymphocyte subpopulations. | the present study describes the in vivo effects of anti-leprosy drugs on rat peritoneal macrophages and t-cell homeostasis. it was observed that bcg-elicited rat peritoneal macrophages produced more h2o2 and expressed more ia antigen on their cell surfaces compared with resident peritoneal macrophages. furthermore, elicited macrophages isolated from rats administered multidrug therapy (mdt), consisting of dapsone, clofazimine and rifampicin in high dose (10 x mdt) released more o2-. on the contr ... | 1992 | 1325957 |
| the administration of foot and mouth disease vaccine with oil adjuvant and its influence on the diagnosis of bovine tuberculosis. | the possible influence of vaccination with oil adjuvanted foot and mouth disease vaccines on the tuberculin response was investigated in 32 normal guinea pigs and 190 non-tuberculous bovines. circulating anti-mycobacterium bovis igg antibodies were analysed by an enzyme-linked immunosorbent assay (elisa), in order to determine the effect of the vaccination on the humoral response against mycobacteria in cattle. control animals were either nonvaccinated or injected with aluminium hydroxide adjuva ... | 1992 | 1335306 |
| expression of escherichia coli beta-galactosidase in mycobacterium bovis bcg using an expression system isolated from mycobacterium paratuberculosis which induced humoral and cellular immune responses. | a promoter sequence, pan, was isolated from mycobacterium paratuberculosis and characterized. this promoter lies adjacent to, and outside, the 3' end of an is900 insertion element. is900 contains an open reading frame, orf2, on the complementary strand which codes for the putative transposase of this insertion sequence. a dna fragment containing pan and part of orf2 was fused to the lacz gene and inserted into the replicative shuttle vector prr3. mycobacterium smegmatis and mycobacterium bovis b ... | 1992 | 1336563 |
| immunosuppression and alteration of resistance to pulmonary tuberculosis in guinea pigs by protein undernutrition. | the impact of chronic moderate protein deficiency on resistance to pulmonary tuberculosis was studied in a guinea pig model. inbred and outbred guinea pigs were maintained on isocaloric diets containing 30% or 10% ovalbumin, vaccinated with mycobacterium bovis bcg vaccine and infected by the respiratory route with virulent mycobacterium tuberculosis. protein deficiency was associated with significant loss of dermal tuberculin hypersensitivity, reduced purified protein derivative (ppd)-driven lym ... | 1992 | 1347317 |
| compartmentalization of the cellular immune response to the recombinant 65 kda protein of mycobacterium bovis bcg in patients with tuberculous pleuritis. | the recombinant 65 kda mycobacterial protein of m. bovis bcg has been shown to be immunodominant in mice immunized with m. tuberculosis. little is known about reactivity to this antigen in patients with tuberculous pleuritis. in this study therefore, pleural effusion and autologous peripheral blood lymphocytes obtained from patients with tuberculous and nontuberculous pleuritis were stimulated in-vitro with the recombinant 65 kda antigen. proliferation was assessed by 3[h] thymidine incorporatio ... | 1992 | 1349232 |
| insertion element is1081-associated restriction fragment length polymorphisms in mycobacterium tuberculosis complex species: a reliable tool for recognizing mycobacterium bovis bcg. | recently, the insertion element is1081 from mycobacterium bovis was identified. in this study, the usefulness of is1081 in the epidemiology of tuberculosis was investigated. the host range of this insertion sequence was found to be restricted exclusively to the group of mycobacterium tuberculosis complex bacteria, whereas none of the 10 mycobacterial species which do not belong to the m. tuberculosis complex contained is1081-homologous dna. all 99 m. tuberculosis complex strains investigated car ... | 1992 | 1352785 |
| evidence for an alpha/beta t cell-independent mechanism of resistance to mycobacteria. bacillus-calmette-guerin causes progressive infection in severe combined immunodeficient mice, but not in nude mice or in mice depleted of cd4+ and cd8+ t cells. | depleting thymectomized mice of cd4+ t cells, or cd4+ plus cd8+ t cells, rendered them incapable of resolving bacillus-calmette-guerin (bcg) infection in their lives, spleens, kidneys, and lungs. however, it did not render them incapable of stabilizing infection in the latter three organs after an initial period of bcg growth. athymic nude mice showed a similar capacity to control bcg growth in these organs after a certain stage of infection. in contrast, congenitally severe combined immunodefic ... | 1992 | 1354243 |
| immunotherapy of breast cancer. | | 1992 | 1355996 |
| a general method to generate dna probes for microorganisms. | we present a method that permits the rapid generation of dna probes for eubacteria. in the procedure the variable regions for the 16s rrna genes are amplified using polymerase chain reaction (pcr) technology and primers based on the conserved regions of these genes. following sequencing of the variable regions, a choice is possible for a probe specific for that organism. no knowledge of the molecular biology of the microorganism is required prior to the application of this approach. the generali ... | 1990 | 1369980 |
| mycobacteria and human heat shock protein-specific cytotoxic t lymphocytes in rheumatoid synovial inflammation. | to study the cytotoxic capacity of mycobacteria-specific t lymphocyte lines and clones from sites of inflammation in patients with rheumatoid arthritis (ra). we also studied antigen specificity, surface phenotype, expression of t cell receptors (tcr), and hla restriction. | 1992 | 1371388 |
| induction of t-cell-mediated immunity against metha fibrosarcoma by intratumoral injections of a bacillus calmette-guérin nucleic acid fraction. | my-1, which consists of dna and rna extracted and purified from bacillus calmette-guérin (bcg), has been shown to have strong antitumor activity against various experimental tumors. to examine the role of t cells in the antitumor mechanism of my-1, the effect of my-1 injection on the development of tumor-specific immunity against metha fibrosarcoma was investigated. my-1 injections inhibited tumor growth less effectively in t-cell-deficient nude mice than in normal balb/c mice. metha tumor growt ... | 1992 | 1371719 |
| cd4+ t cells are required for antigen-specific recruitment of neutrophils by bcg-immune spleen cells. | mycobacterium bovis bacillus calmette-guérin (bcg)-immune spleen cells co-inoculated into the peritoneal cavity of normal mice with bcg sonicate protein as antigen could induce an antigen-specific recruitment of neutrophils, dependent on the antigen dose and cell number. this response was significantly reduced by anti-t lymphocyte and anti-cd4 treatment of the immune spleen cells prior to the inoculation. removal of adherent or phagocytic cells or lysis of b cells, had no significant effect. kil ... | 1992 | 1374053 |
| ifn-gamma-producing ability as a possible marker for the protective t cells against mycobacterium bovis bcg in mice. | we searched for a functional marker with which t cells mediating acquired cellular resistance (acr) can be discriminated from those mediating delayed-type hypersensitivity (dth) in mice immunized with mycobacterium bovis bcg. four wk after injection of the mice with 10(5) viable bcg (vbcg) cells emulsified in ifa, a passive transfer experiment revealed that t cells mediating dth as well as acr, which we designated tacr, were generated in the spleen. in contrast, t cells mediating only dth (tdth) ... | 1992 | 1374103 |
| unique palindromic sequences in synthetic oligonucleotides are required to induce ifn [correction of inf] and augment ifn-mediated [correction of inf] natural killer activity. | thirty-mer single-stranded oligonucleotides, with a sequence chosen from the known cdna encoding the 64-kda protein named ag a or the mpb-70 protein of mycobacterium bovis bcg and the human cellular proteins such as complement component 1 inhibitor and ig rearranged lambda-chain, were used to dissect the capability to induce ifn and to augment nk cell activity of mouse spleen cells by coincubation in vitro. three with the hexamer palindromic sequence as gacgtc were active, whereas two kinds of o ... | 1992 | 1376349 |