| isolation of non-sporing anaerobic rods from infections in children. | from 1974 to 1994, 2033 microbiological specimens from children were submitted for cultures for anaerobic bacteria. fifty-seven isolates of bifidobacterium spp. were obtained from 55 (3%) children, 67 isolates of eubacterium spp. from 65 (3%) children and 41 isolates of lactobacillus spp. from 40 (2%) children. most bifidobacterium isolates were from chronic otitis media, abscesses, peritonitis, aspiration pneumonia and paronychia. most eubacterium isolates were from abscesses, peritonitis, decu ... | 1996 | 8667406 |
| tungsten in biological systems. | tungsten (atomic number 74) and the chemically analogous and very similar metal molybdenum (atomic number 42) are minor yet equally abundant elements on this planet. the essential role of molybdenum in biology has been known for decades and molybdoenzymes are ubiquitous. yet, it is only recently that a biological role for tungsten has been established in prokaryotes, although not as yet in eukaryotes. the best characterized organisms with regard to their metabolism of tungsten are certain specie ... | 1996 | 8672295 |
| cloning, sequencing and expression of the uvra gene from an extremely thermophilic bacterium, thermus thermophilus hb8. | one of the most important dna repair systems is the nucleotide (nt) excision repair system. the uvr a gene, which plays an essential role in the prokaryotic excision repair system, was cloned from an extremely thermophilic eubacterium, thermus thermophilus (tt) hb8, and its nt sequence was determined. in the amino acid (aa) sequence of tt uvra, a characteristic duplicated structure, two nt-binding consensus sequences (walker's a-type motif) and two zinc finger dna-binding motifs were found. the ... | 1996 | 8675016 |
| antitumor mechanisms of eubacterium lentum and its components. | in the present study, some antitumor mechanisms of eubacterium lentum (tyh-11) and bacterial components having antitumor effects were investigated. e.lentum induced maximum nk cell activity in c3h/he mice on day 1 after injection (90.6% against 33.9% of control at e:t ratio 50:1) and the activity was kept at a level of 48.6% on day 7. tumoricidal peritoneal macrophages were induced 9 days after e.lentum injection into balb/c mice (56.2% against 10.1% control at e:t ratio 10:1). tumoricidal macro ... | 1995 | 8703241 |
| effect of orally administered eubacterium coprostanoligenes atcc 51222 on plasma cholesterol concentration in laying hens. | thirty normocholesterolemic laying hens were used to investigate the effect of oral administration of eubacterium coprostanoligenes on plasma cholesterol concentrations. hens were divided randomly into three treatment groups (active, inactive, and control) with 10 hens in each group. the active group received 0.5 ml of e. coprostanoligenes suspension (approximately 2 x 10(7) cells per milliliter) daily for 4 wk; the inactive group received the same dosage of killed (boiled) bacterial suspension; ... | 1996 | 8737839 |
| polymerase chain reaction-based detection of bacteria in semen. | to determine if the presently used bacterial detection techniques provide accurate and complete profiles of microorganisms found in human semen. | 1996 | 8751749 |
| a eubacterial mycobacterium tuberculosis trna synthetase is eukaryote-like and resistant to a eubacterial-specific antisynthetase drug. | we report here the cloning and primary structure of mycobacterium tuberculosis isoleucyl-trna synthetase. the predicted 1035-amino acid protein is significantly more similar in sequence to eukaryote cytoplasmic than to other eubacterial isoleucyl-trna synthetases. this similarity correlates with the enzyme being resistant to pseudomonic acid a, a potent inhibitor of escherichia coli and other eubacterial isoleucyl-trna synthetases, but not of eukaryote cytoplasmic enzymes. consistent with its eu ... | 1996 | 8756461 |
| asn-trna in lactobacillus bulgaricus is formed by asparaginylation of trna and not by transamidation of asp-trna. | in many organisms (e.g., gram-positive eubacteria) gin-trna is not formed by direct glutaminylation of trnagln but by a specific transamidation of glu-trnagln. we wondered whether a similar transamidation pathway also operates in the formation of asn-trna in these organisms. therefore we tested in s-100 preparations of lactobacillus bulgaricus, a gram-positive eubacterium, for the conversion by an amidotransferase of [14c]asp-trna to [14c]asn-trna. as no transamidation was observed, we searched ... | 1996 | 8758990 |
| mechanism of cholesterol reduction to coprostanol by eubacterium coprostanoligenes atcc 51222. | the mechanism of reduction of cholesterol to coprostanol by eubacterium coprostanoligenes atcc 51222 was studied by incubating the bacterium with a mixture of alpha- and beta-isomers of [4-3h,4-14c]cholesterol. coprostanol, isolated after incubation of [4-3h,4-14c]cholesterol in a growth medium under anaerobic conditions, retained 97% of the tritium originally present in cholesterol. the majority of this tritium (64%), however, was in the c-6 position in coprostanol, which showed that the conver ... | 1996 | 8789734 |
| developmental control of the heat-shock stress regulon in streptomyces coelicolor. | in the differentiating eubacterium streptomyces coelicolor, nutritional imbalances activate a developmental programme which involves the heat-shock stress regulon. in liquid batch cultures, the growth curve could be separated into four components: rapid growth 1 (rg1), transition (t), rapid growth 2 (rg2) and stationary (s). patterns of gene expression in cultures subjected to heat shock in various phases were recorded on two-dimensional gels and analysed using advanced statistical methods. the ... | 1995 | 8801427 |
| expression and characterization of a c24 bile acid 7 alpha-dehydratase from eubacterium sp. strain vpi 12708 in escherichia coli. | the intestinal bacterium eubacterium sp. strain vpi 12708 has been shown to have a bile acid 7 alpha/7 beta-dehydroxylation pathway. a large bile acid inducible (bai) operon encoding at least 9 open reading frames has been cloned and sequenced from this bacterium. the baie gene from this operon has been subcloned and expressed in e. coli and found to encode a bile acid 7 alpha-dehydratase (ba7 alpha d). the purified ba7 alpha d was shown to have a calculated subunit mass of 19 kd and a relative ... | 1996 | 8808760 |
| sequence of a giardia lamblia gene coding for the glycolytic enzyme, pyruvate,phosphate dikinase. | the sequence of the gene coding for pyruvate,phosphate dikinase (ec 2.7.9.1) in giardia lamblia (syn. g. duodenalis) has been established. the deduced amino acid sequence is very similar to all of its known homologs from the protist, entamoeba histolytica, the eubacterium, clostridium symbiosum and plant chloroplasts. phylogenetic reconstruction with neighbor-joining and maximum parsimony methods reveals that the sequences form two clades, one comprising the anaerobic microorganisms, and the oth ... | 1996 | 8813667 |
| bacteriologic findings in tonsillitis and pericoronitis. | bacteriologic samples from 31 young men were cultured quantitatively for aerobes and anaerobes; these samples included 31 specimens of tonsils (16 infected and 15 healthy), 16 specimens from pericoronal pockets of lower third molars (11 infected and 5 symptom-free), and 6 postoperative specimens from lower-third-molar extraction sockets. anaerobes were isolated more often from infected third molars than from infected tonsils (14.5 isolates vs. 8.4 isolates, respectively; p < .001). infected tons ... | 1996 | 8816129 |
| a purgative action of barbaloin is induced by eubacterium sp. strain bar, a human intestinal anaerobe, capable of transforming barbaloin to aloe-emodin anthrone. | orally administered barbaloin (100 mg/kg) did not induce any diarrhea in male wistar rats, in spite of severe diarrhea with sennoside b (40 mg/kg). also, in gnotobiote rats mono-associated with peptostreptococcus intermedius, a human intestinal anaerobe capable of reducing sennidins to rhein anthrone, barbaloin did not induce diarrhea; the faecal water content (71.9%) 8 h after the administration of barbaloin was not increased, compared with that (73.9%) just before the treatment. however, sever ... | 1996 | 8820926 |
| molecular cloning and expression of the spsb gene encoding an essential type i signal peptidase from staphylococcus aureus. | the gene, spsb, encoding a type i signal peptidase has been cloned from the gram-positive eubacterium staphylococcus aureus. the gene encodes a protein of 191 amino acid residues with a calculated molecular mass of 21,692 da. comparison of the protein sequence with those of known type i signal peptidases indicates conservation of amino acid residues known to be important or essential for catalytic activity. the enzyme has been expressed to high levels in escherichia coli and has been demonstrate ... | 1996 | 8824617 |
| pyomyositis due to eubacterium lentum and streptococcus constellatus from a periodontal source. | | 1996 | 8824994 |
| purification and characterization of a geniposide-hydrolyzing beta-glucosidase from eubacterium sp. a-44, a strict anaerobe from human feces. | a geniposide-hydrolyzing beta-glucosidase was discovered in eubacterium sp. a-44, a human intestinal anaerobe. the enzyme was intracellularly distributed in the bacterium, and purified to homogeneity from the extract using butyl-toyopearl 650m, sephacryl s-300, hydroxyapatite and chromatofocusing column chromatography. the enzyme was a single polypeptide chain with the molecular weight of 90 kda and the n-terminal amino acid sequence initiated from methionine up to the 29th residue did not show ... | 1995 | 8845799 |
| phylogeny of oral asaccharolytic eubacterium species determined by 16s ribosomal dna sequence comparison and proposal of eubacterium infirmum sp. nov. and eubacterium tardum sp. nov. | 16s rrna gene sequences of eubacterium brachy, eubacterium nodatum, eubacterium saphenum, eubacterium timidum, and two previously unnamed taxa were determined. the results of a phylogenetic analysis indicated that all of the strains sequenced belonged to a deep branch of the low-g+c-content gram-positive group. the levels of 16s ribosomal dna sequence similarity between species were low, suggesting that a number of genera may be represented in this group. the representatives of the two unnamed t ... | 1996 | 8863423 |
| phylogenetic relationships of the genera acetobacterium and eubacterium sensu stricto and reclassification of eubacterium alactolyticum as pseudoramibacter alactolyticus gen. nov., comb. nov. | 16s rrna gene sequences of the type strains of the seven previously described acetobacterium species were determined. the acetobacterium species were found to form a tight phylogenetic cluster within the clostridium subphylum of the gram-positive bacteria. within this subphylum these organisms belong to cluster xv as defined by collins et al. (m.d. collins, p.a. lawson, a. willems, j.j. cordoba, j. fernandez-garayzabal, p. garcia, j. cai, h. hippe, and j. a. e. farrow, int. j. syst. bacteriol. 4 ... | 1996 | 8863439 |
| eubacterium exiguum sp. nov., isolated from human oral lesions. | eubacterium exiguum sp. nov. is the name proposed for organisms formerly described as eubacterium group s strains and similar bacteria isolated from various types of oral lesions. this new species was established on the basis of the results of dna-dna hybridization experiments and dna base composition determinations (g + c contents, 60 to 64 mol%). the results of an api zym analysis, western blotting (immunoblotting) reactions, and phenotypic tests are also given. the type strain of e. exiguum i ... | 1996 | 8863445 |
| the origin of the eukaryotic cell. | molecular sequence data are beginning to provide important insights into the evolutionary origin of eukaryotic cells. global phylogenies of numerous protein sequences indicate that the eukaryotic cell nucleus is a chimera, which has received major contributions from both a gram-negative eubacterium and an archaebacterium. recent studies also indicate that the formation of the nuclear envelope and the endoplasmic reticulum was accompanied by duplication of genes for the molecular chaperone protei ... | 1996 | 8871398 |
| structure-activity studies on c-terminal hirudin peptides containing sulfated tyrosine residues. | to clarify the role of the negative charge of the c-terminal region of hirudin, we chemically synthesized the c-terminal peptide of hirudin variant-1 (hv-1), hv-1-(54-65), and its analogs, [e61y,e62y]hv-1-(54-65) and [e62y]hv-1-(54-65), and then sulfated the tyr residue(s) in these peptides by both enzymic and chemical methods. enzymic o-sulfation of tyr residues in the peptides by use of sulfotransferase isolated from eubacterium a-44 allowed us to produce four kinds of the sulfated peptide, wh ... | 1996 | 8872535 |
| role of intestinal bacteria in ileal ulcer formation in rats treated with a nonsteroidal antiinflammatory drug. | the role of intestinal bacteria in induction and repression of ulcer formation in the ileum of rats treated with one of the nonsteroidal antiinflammatory drugs (nsaids), 5-bromo-2-(4-fluorophenyl)-3-(4-methylsulfonylphenyl) thiophene (bfmet), was examined in this study. bfmet was administered by intragastric gavage once at doses of 500-1,500 mg/kg of body weight to wistar rats treated with and without antibiotics (bacitracin, neomycin, streptomycin), germ-free rats and gnotobiotic rats, and 72 h ... | 1996 | 8887349 |
| the effect of cyclosporin-a on the oral microflora at gingival sulcus of the ferret. | the effect of cyclosporin-a (cya) on the dentogingival flora of ferrets with healthy and experimentally induced periodontal breakdown was studied. five animals were given 10 mg/kg/d cya. at the start of the experiments (day 0), ligatures were placed around 4 teeth in the right upper and lower jaws; corresponding contralateral teeth on the left side served as control. on days 0 and 28 (end of the experiment), microbiological samples were collected from the gingival sulcus of the experimental and ... | 1996 | 8891937 |
| demethylation of dimethylsulfoniopropionate to 3-s-methylmercaptopropionate by marine sulfate-reducing bacteria. | the initial step in the anaerobic degradation of the algal osmolyte dimethylsulfoniopropionate (dmsp) in anoxic marine sediments involves either a cleavage to dimethylsulfide and acrylate or a demethylation to 3-s-methylmercaptopropionate. thus far, only one anaerobic bacterial strain has been shown to carry out the demethylation, namely, desulfobacterium sp. strain pm4. the aims of the present work were to study how common this property is among certain groups of anaerobic bacteria and to obtai ... | 1996 | 8899985 |
| protein-encoding genes in the sulfothermophilic archaea sulfolobus and pyrococcus. | a number of unrelated protein-encoding genes from sulfothermophilic archaea, sulfolobus acidocaldarius, sulfolobus solfataricus, pyrococcus furiosus and pyrococcus woesei, has been analyzed. in the sulfolobus genus, the content of a + t is significantly higher than that of c + g and the base usage follows the order, a > t > g > c. in pyrococcus, the a + t content is also higher than that of c + g, but with lower values; in the order of base usage, g precedes t. the codon usage of these sulfother ... | 1996 | 8918227 |
| pcr detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples. | pcr procedures based on 16s rrna gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. fusobacterium prausnitzii, peptostreptococcus productus, and clostridium clostridiiforme had high pcr titers (the maximum dilutions for positive pcr results ranged from 10(-3) to 10(-8)) in all of the human an ... | 1996 | 8919784 |
| [occurrence of non-spore forming anaerobic bacteria in the upper airways of patients with chronic obstructive pulmonary disease]. | the aim of this study was to assess the occurrence of anaerobic non-sporeforming bacteria upper airways flora in the exacerbation of copd. sputum from 35 copd patients was sent to the laboratory in sterile, filled with co2 containers and cultured under anaerobic conditions. in all patients bacteriological tests were positive. the most common anaerobic bacteria were as follows: peptostreptococcus - in 28 pts, fusobacterium - in 27 pts, bacteroides - in 26 pts and prevotella in 24. less common wer ... | 1996 | 8926767 |
| characterization of proteolytic activities of rumen bacterial isolates from forage-fed cattle. | the proteolytic activities of eight strains of ruminal bacteria isolated from new zealand cattle were characterized with respect to their cellular location, response to proteinase inhibitors and hydrolysis of artificial proteinase substrates. the streptococcus bovis strains had predominantly cell-bound activity, which included a mixture of serine and cysteine-type proteinases which had high activity against leucine p-nitroanilide (lpna). the eubacterium strains had a mainly cell-associated activ ... | 1996 | 8939033 |
| the establishment of a community of oral bacteria that controls the growth of candida albicans in a chemostat. | the purpose of this study was to establish and identify a community of oral bacteria that controls the growth of candida albicans in the chemostat. the chemostat was operated under glucose-limiting conditions at a dilution rate of 0.05 h-1 and inoculated with a yeast-free suspension of a tongue scraping. after a steady state had been reached, it was inoculated with c. albicans to establish the yeast and determine whether its growth could be contained. the steady-state community comprised the spe ... | 1996 | 8941776 |
| alteration of bile acid metabolism by cimetidine in healthy humans. | to clarify an effect of cimetidine on bile acid metabolism, we evaluated whether an increased deconjugation of bile acids would occur in healthy humans who have received cimetidine. we examined: 1) whether healthy volunteers taking cimetidine would have positive bile acid breath tests because of bacterial overgrowth in the jejunum; 2) whether the isolated bacteria would exhibit deconjugation ability; and 3) whether a change in gastric ph was related to the bacterial overgrowth. | 1996 | 8952227 |
| mannitol, a novel bacterial compatible solute in pseudomonas putida s12. | the aim of this study was to identify the compatible solutes accumulated by pseudomonas putida s12 subjected to osmotic stress. in response to reduced water activity, p. putida s12 accumulated nalpha-acetylglutaminylglutamine amide (naggn) simultaneously with a novel compatible solute identified as mannitol (using 13c- and 1h-nuclear magnetic resonance, liquid chromatography-mass spectroscopy and high-performance liquid chromatography methods) to maximum concentrations of 74 and 258 micromol g ( ... | 1996 | 8955280 |
| sequencing and expression of a gene encoding a bile acid transporter from eubacterium sp. strain vpi 12708. | eubacterium sp. strain vpi 12708 expresses inducible bile acid 7alpha-dehydroxylation activity via a multistep pathway. the genes encoding several of the inducible proteins involved in the pathway have been previously mapped to a bile acid-inducible (bai) operon in eubacterium sp. strain vpi 12708. we now report the cloning, sequencing, and characterization of the baig gene, which is part of the bai operon. the predicted amino acid sequence of the baig polypeptide shows significant homology to s ... | 1996 | 8955384 |
| [cerebral abscess in childhood: a report of 5 cases]. | | 1996 | 8967649 |
| introducing mutations into a chromosomal rrna gene using a genetically modified eubacterial host with a single rrna operon. | gene-inactivation techniques were employed to construct a eubacterial organism harbouring a single functional rrna operon. this mutant of mycobacterium smegmatis permits replacement of the single remaining rrna operon with a homologous fragment from a vector-borne gene. by homologous recombination with the chromosome a plasmid-borne rdna segment with resistance markers substitutes for the corresponding region of the chromosomal rrna operon, resulting in a homogeneous population of mutated riboso ... | 1996 | 8971706 |
| purification and characterization of extremely thermostable beta-mannanase, beta-mannosidase, and alpha-galactosidase from the hyperthermophilic eubacterium thermotoga neapolitana 5068. | thermostable and thermoactive beta-mannanase (1,4-beta-d-mannan mannanohydrolase [ec 3.2.1.78]), beta-mannosidase (beta-d-mannopyranoside hydrolase [ec 3.2.1.25]) and alpha-galactosidase (alpha-d-galactoside galactohydrolase [ec 3.2.1.22]) were purified to homogeneity from cell extracts and extracellular culture supernatants of the hyperthermophilic eubacterium thermotoga neapolitana 5068 grown on guar gum-based media. the beta-mannanase was an extracellular monomeric enzyme with a molecular mas ... | 1997 | 8979350 |
| 16s rdna analysis of butyrivibrio fibrisolvens: phylogenetic position and relation to butyrate-producing anaerobic bacteria from the rumen of white-tailed deer. | complete 16s rdna sequences of six strains of butyrivibrio fibrisolvens, including the type strain (atcc 19171), were determined. the type strain was found to have less than 89% sequence similarity to the other isolates that were examined. the five plasmid-bearing strains formed a closely related cluster and three of these strains (ob156, ob157 and ob192) were very highly related (> 99%), indicating that they are isolates of the same genomic species. the phylogenetic position of butyrivibrio was ... | 1996 | 8987694 |
| systematic study of the genus vogesella gen. nov. and its type species, vogesella indigofera comb. nov. | a blue-pigmented colony that had a metallic copper-colored sheen was isolated in 1973 from a standard spread plate count preparation of oxidation pond sediment. over the next 11 years, an additional 12 strains of blue-pigmented bacteria were isolated from freshwater samples and compared to several reference strains of bacteria. morphological and biochemical tests revealed that these 13 isolates were very similar to [pseudomonas] indigofera atcc 19706t (t = type strain) and atcc 14036. a numerica ... | 1997 | 8995797 |
| bacterial colonization on internal surfaces of brånemark system implant components. | the aim of the present study was to examine the microbiota on the internal surface of the components of 28 brånemark implants in 10 partially edentulous patients who had been treated with 1 fixed partial prostheses each. the prostheses had been in function for 1 to 8 years. the fixed prostheses were checked for mobility and removed. the abutment screws were loosened and classified as stable, easily removed or loose. then, bacterial samples were obtained from the various internal surfaces of the ... | 1996 | 9002826 |
| three subtypes of the tet(m) gene identified in bacterial isolates from periodontal pockets. | the tet(m) genes were characterized from 84 isolates of 10 different bacterial species isolated from the periodontal pockets of 16 patients with periodontal disease. a 740 bp polymerase chain reaction product from the hypervariable region of the tet(m) structural gene was cleaved with the restriction enzymes alui and hinfi. three different restriction patterns were identified for each of the two enzymes. by dna sequencing, using a direct solid-phase automated sequencing method, the isolates coul ... | 1996 | 9028254 |
| molecular identification of a novel protein that regulates biogenesis of photosystem i, a membrane protein complex. | photosystem i (psi) is a multisubunit pigment-protein complex in the thylakoid membranes of cyanobacteria and chloroplasts. bp26, a random photosynthesis-deficient mutant strain of the cyanobacterium synechocystis 6803 has a severely reduced psi content, whereas its photosystem ii is present in normal amount. the bp26 mutant is complemented by a novel gene, btpa, that encodes a 30-kda protein. in this strain, a missense mutation in the btpa gene, resulting in the replacement of a valine by a gly ... | 1997 | 9045660 |
| assessment of fecal bacteria with bile acid 7 alpha-dehydroxylating activity for the presence of bai-like genes. | eubacterium sp. strain vpi 12708 has several bile acid-inducible (bai) genes which encode enzymes in the bile acid 7 alpha-dehydroxylation (7 alpha deoh) pathway. twelve 7 alpha deoh-positive intestinal bacterial strains were assayed for 7 alpha deoh activity, and 13 strains were tested for hybridization with bai genes. cholic acid 7 alpha deoh activity varied greatly (> 100-fold) among these strains. southern blot experiments showed that dna prepared from 7 of 13 strains hybridized with at leas ... | 1997 | 9055436 |
| a comparison of susceptibility results of the bacteroides fragilis group and other anaerobes by traditional mic results and statistical methods. | antimicrobial susceptibility is compared by mic values reflecting activity by weight and/or percentage of strains resistant. this study establishes these parameters in vitro in the traditional fashion and extends analysis statistically for various groups of anaerobic bacteria. mode mic, mic50, mic90 values and percentage of strains resistant indicated comparable activity for most of the beta-lactams. geometric and arithmetic mics suggested ceftizoxime to be the most active beta-lactam. statistic ... | 1997 | 9096180 |
| crystal structure of glutamate dehydrogenase from the hyperthermophilic eubacterium thermotoga maritima at 3.0 a resolution. | the extremely thermostable glutamate dehydrogenase from the hyperthermophilic bacterium thermotoga maritima has been crystallized and the three-dimensional structure has been determined by x-ray diffraction methods. crystals up to a maximum size of 1.2 mm have been grown in 3% polyethylene glycol, 120 mm ammonium acetate and 50 mm bis-tris propane (ph 6.5). the enzyme crystallized in the trigonal space group p3(1)21 with the cell dimensions a = b = 147.3 a, c = 273.6 a. the diffraction limit of ... | 1997 | 9135121 |
| isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene. | tetrachloroethene is a prominent groundwater pollutant that can be reductively dechlorinated by mixed anaerobic microbial populations to the nontoxic product ethene. strain 195, a coccoid bacterium that dechlorinates tetrachloroethene to ethene, was isolated and characterized. growth of strain 195 with h2 and tetrachloroethene as the electron donor and acceptor pair required extracts from mixed microbial cultures. growth of strain 195 was resistant to ampicillin and vancomycin; its cell wall did ... | 1997 | 9171062 |
| protein degradation by prevotella intermedia and actinomyces meyeri supports the growth of non-protein-cleaving oral bacteria in serum. | the proteolytic activities of oral bacteria are thought to play an important role in the aetiology of dental abscesses. bacteria-derived proteases may contribute to tissue destruction, and are likely to impair host defence by degrading immunoglobulins and complement. degraded periodontal tissue and tissue fluid are likely to constitute essential sources of nutrients in the abscess. tissue fluid, which is derived from serum, is rich in protein and poor in carbohydrate, suggesting that breakdown o ... | 1997 | 9178115 |
| associations of endodontic symptoms and signs with particular combinations of specific bacteria. | significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. the prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of t ... | 1996 | 9206427 |
| kinesin light chain in a eubacterium. | a eubacterial homolog of a kinesin light chain gene has been isolated and characterized from the cyanobacterium plectonema boryanum. although the eubacterial and eukaryotic kinesin light chains are highly similar in amino acid sequence, the eubacterial sequence differs in several distinguishing structural features, including the absence of a putative pest domain and the presence of additional highly conserved imperfect tandem repeats. two soluble kinesin light chain antigens have been identified ... | 1997 | 9212172 |
| porin from the halophilic species ectothiorhodospira vacuolata: cloning, structure of the gene and comparison with other porins. | the gene coding for the anion-specific porin of the halophilic eubacterium ectothiorhodospira (ect.) vacuolata was cloned and sequenced, the first such gene so analyzed from a purple sulfur bacterium. it encodes a precursor protein consisting of 374 amino acid (aa)-residues including a signal peptide of 22-aa residues. comparison with aa sequences of porins from several other members of the proteobacteria revealed little homology. only two regions showed local homology with the previously sequen ... | 1997 | 9218724 |
| a dnab intein in rhodothermus marinus: indication of recent intein homing across remotely related organisms. | a dnab gene encoding a homologue of the escherichia coli dna helicase dnab was cloned and sequenced in the thermophilic eubacterium rhodothermus marinus, predicting a dnab protein that harbors an intein. this dnab intein is 428 amino acid residues long, has several putative intein sequence motifs (including two putative endonuclease motifs), and is capable of protein splicing when produced in e. coli cells. the r. marinus dnab intein is a close homologue of a dnab intein in the cyanobacterium sy ... | 1997 | 9223276 |
| uptake and processing of dna by acinetobacter calcoaceticus--a review. | in natural transformation, dna in the form of macromolecular fragments can be translocated across the cell envelope of prokaryotic microorganisms. during the past two decades, several, largely mutually contradictory, hypotheses have been forwarded to explain the molecular mechanism and bioenergetics of this translocation process. other biomacromolecules are translocated across the bacterial cell envelope as well, such as polysaccharides and proteins, the latter for instance in the process of the ... | 1997 | 9224889 |
| purification and characterization of a groel homologue from the moderately eubacterial halophile pseudomonas sp. #43. | we have purified to apparent homogeneity and characterized a molecular chaperonin groel homologue (hpgroel) from a moderately halophilic eubacterium, pseudomonas sp. #43. although this halophilic bacterium requires 1-2 m nacl for growth, hpgroel did not require a high concentration of salt for its stability, atpase activity and refold-promoting activity for denatured protein. the atpase activity was even more halo-sensitive than that of groel from escherichia coli. the hpgroel protein promotes m ... | 1997 | 9231426 |
| synthesis of 4-aza-5,6-dimethylbenzimidazole and biosynthetic preparation of 4- and 7-aza-5,6-dimethylbenzimidazolylcobamide. | we report on the preparation of 4-aza-5,6-dimethylbenzimidazolylcobamide and 5,6-dimethyl-7-azabenzimidazolylcobamide. these vitamin b12-analogs were required as reference compounds for comparison with a corrinoid previously isolated in small amounts for eubacterium limosum grown in the presence of 4(5)-aminoimidazole. 4(7)-aza-5,6-dimethylbenzimidazole was synthesized from n-1-benzyl-4-nitroimidazole which was reduced to n-1-benzyl-4-aminoimidazole and condensed with 1-dimethylamino-2-methylbut ... | 1997 | 9232888 |
| the sequences of heat shock protein 40 (dnaj) homologs provide evidence for a close evolutionary relationship between the deinococcus-thermus group and cyanobacteria. | the genes encoding for heat shock protein 40 (hsp40 or dnaj) homologs were cloned and sequenced from the archaebacterium halobacterium cutirubrum and the eubacterium deinococcus proteolyticus to add to sequences from the gene banks. these genes were identified downstream of the hsp70 (or dnak) genes in genomic fragments spanning this region and, as in other prokaryotic species, hsp70-hsp40 genes are likely part of the same operon. the hsp40 homolog from d. proteolyticus was found to be lacking a ... | 1997 | 9236279 |
| genetic manipulation of anaerobic cellulolytic bacteria. | transposon tn1545 was introduced into the chromosome of the ruminal cellulolytic bacterium eubacterium cellulosolvens. this was achieved by conjugal transfer of the transposon from clostridium beijerinckii at a frequency of about 1 per 10(4) recipient cells. transconjugants of e. cellulosolvens were resistant to both tetracycline and erythromycin. e. cellulosolvens could also serve as a donor for conjugal transfer of tn1545 back into c. beijerinckii. | 1997 | 9274059 |
| [intestinal flora in renal allograft recipients]. | to explore the change of 10 kinds of intestinal flora in renal allograft recipients and its significance. | 1996 | 9275519 |
| inactivation of the 20s proteasome in mycobacterium smegmatis. | the 20s proteasome is an essential component of the cytosolic protein turnover apparatus of eukaryotic cells. in higher eukaryotes, the 20s proteasome is responsible for most cytosolic protein turnover and also generates peptides for subsequent presentation by the mhc class i pathway. structurally, the eukaryotic 20s proteasome is extremely complex, being composed of 14 different subunits. proteasomes with simplified subunit composition have been identified in certain eubacteria and archaebacter ... | 1997 | 9282749 |
| bacillus stearothermophilus as a model to evaluate membrane toxicity of a lipophilic environmental pollutant (ddt). | the thermophilic eubacterium bacillus stearothermophilus has been used as a model system to identify ddt-promoted events in biological membranes putatively related with the insecticide toxicity. two strategies have been approached: a) bacterial growth and viability were followed and the effects of ddt (2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane) determined; b) biophysical studies with fluorescent probes were performed to elucidate the effects of ddt on the organization of the membrane lipid b ... | 1997 | 9294237 |
| clarithromycin-induced digoxin intoxication. | to report a case of clarithromycin-induced digoxin intoxication. | 1997 | 9296239 |
| a dna polymerase iii holoenzyme-like subassembly from an extreme thermophilic eubacterium. | we have purified a novel dna polymerase from thermus thermophilus. this was enabled by use of general gap filling assays to monitor polymerase activity and cross-reactive monoclonal antibodies against the alpha catalytic subunit of e. coli dna polymerase iii holoenzyme to distinguish a novel polymerase from the well characterized dna polymerase i-like thermus thermophilus dna polymerase. two proteins migrating with the polymerase after three chromatographic steps were isolated and subjected to p ... | 1997 | 9299346 |
| cytochrome-c552 from thermus thermophilus: a functional and crystallographic investigation. | the eubacterium thermus thermophilus expresses terminal oxidases of the ba3- and caa3-type. the soluble cytochrome-c552 of this organism has been isolated by a new method and characterized. in contrast to previous studies, but in line with coexpression at low aeration, the cytochrome was unambiguously identified as the substrate of the ba3-oxidase. in the presence of tmpd and ascorbate, biphasic eadie-hofstee plots with kmax = 250 s-1 at 25 degrees c are observed upon addition of cytochrome-c552 ... | 1997 | 9299406 |
| recently described clinically important anaerobic bacteria: taxonomic aspects and update. | a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ... | 1997 | 9310640 |
| purification and reconstitution of na+-translocating vacuolar atpase from enterococcus hirae. | vacuolar atpases make up a family of proton pumps distributed widely from bacteria to higher organisms. an unusual member of this family, a sodium-translocating atpase, has been found in the eubacterium enterococcus hirae. we report here the purification of enterococcal na+-atpase from the plasma membrane of cells, whose atpase content was highly amplified by expression of the cloned ntp operon that encodes this na+-atpase (ntpfikecgabdhj). the purified enzyme appears to consist of nine ntp poly ... | 1997 | 9312089 |
| phenotypic and phylogenetic characterization of some eubacterium-like isolates containing a novel type b wall murein from human feces: description of holdemania filiformis gen. nov., sp. nov. | a group of eubacterium-like strains (designated group s14), isolated from the feces of healthy people, was characterized by biochemical tests, fatty acid analysis, cell wall murein analysis, and 16s rdna analysis. our results indicate that group s14 is phylogenetically a member of the clostridium subphylum of the gram-positive bacteria. despite a phenotypic resemblance to the genus eubacterium, group s14 was shown to be phylogenetically distantly related to the type species of the genus, eubacte ... | 1997 | 9336928 |
| in-vitro activity of spiramycin and metronidazole alone or in combination against clinical isolates from odontogenic abscesses. | one hundred and forty-eight isolates of bacteria from 20 intraoral odontogenic abscesses were tested for their susceptibility to spiramycin and metronidazole alone or in combination. all isolates, except rothia spp. (one), enterococcus avium (three), haemophilus parainfluenzae (one) and staphylococcus aureus (one) were sensitive to spiramycin and/or metronidazole. among the anaerobes, spiramycin as well as metronidazole showed good antimicrobial activity against species of prevotella, eubacteriu ... | 1997 | 9338486 |
| identification of plasmids in the genus marinococcus and complete nucleotide sequence of plasmid ppl1 from marinococcus halophilus. | several plasmids were detected in the gram-positive halophilic eubacterium marinococcus halophilus and in the related strain m52. the complete nucleotide sequence (3874 bp) of one of these plasmids, ppl1, was determined. four major open reading frames were identified. whereas orf3 and orf4 showed no sequence similarities to known proteins, rep displayed a high sequence similarity to replication proteins of rolling circle plasmids. upstream of this orf, a sequence resembling the double-strand ori ... | 1997 | 9339468 |
| definition of mycobacterium tuberculosis culture filtrate proteins by two-dimensional polyacrylamide gel electrophoresis, n-terminal amino acid sequencing, and electrospray mass spectrometry. | a number of the culture filtrate proteins secreted by mycobacterium tuberculosis are known to contribute to the immunology of tuberculosis and to possess enzymatic activities associated with pathogenicity. however, a complete analysis of the protein composition of this fraction has been lacking. by using two-dimensional polyacrylamide gel electrophoresis, detailed maps of the culture filtrate proteins of m. tuberculosis h37rv were generated. in total, 205 protein spots were observed. the couplin ... | 1997 | 9353028 |
| the role of ribosomal rnas in macrolide resistance. | macrolides are bacteriostatic antibiotics which interfere with the peptidyltransfer function of the ribosome. we have investigated the molecular mechanisms underlying macrolide resistance in mycobacterium smegmatis, an eubacterium carrying two rrna operons. surprisingly, drug resistance was associated not with alterations in ribosomal proteins, but with a single point mutation in the peptidyltransferase region of one of the two 23s rna genes, i.e. a2058-->g or a2059-->g. this mutation resulted i ... | 1997 | 9402018 |
| [aerobic and anaerobic bacterial flora in chronic sinusitis in adults]. | the aim of the study was to analyse microbiologically samples obtained from 30 patients aged from 21 to 73 years treated for chronic sinusitis. aerobic bacteria only were isolated in 16 patients (53%), and anaerobic organisms only in 5 patients (17%). mixed aerobic and anaerobic isolates were recovered from 9 patients (30%). the isolated aerobic bacteria were as follows: streptococci from the species streptococcus salivarius, streptococcus anginosus, streptococcus group c, streptococcus sanguis, ... | 1997 | 9411078 |
| molecular cloning and sequence analysis of the lysr gene from the extremely thermophilic eubacterium, thermus thermophilus hb27. | we have isolated a lysine-auxotrophic and kanamycin-resistant mutant from an extreme thermophile, thermus thermophilus hb27. this mutant showed the lysa- or lysr- genotype since it could not grow on the minimal plate which contained diaminopimelic acid. sequence analysis of the clones which could rescue the lys- mutant indicated the lysr gene. the lysr gene overlapped with the rimk gene for the modification enzyme of ribosomal protein s6. in the lys- mutant, the lysr gene was disrupted and the c ... | 1997 | 9418242 |
| the lon protease from mycobacterium smegmatis: molecular cloning, sequence analysis, functional expression, and enzymatic characterization. | we have charterized a mycobacterium smegmatis gene encoding a homolog of the atp-dependent protease lon (la). our identification of a lon homolog, in conjunction with our previous work, identifies m. smegmatis as the first known example of a eubacterium containing both lon and a complete 20s proteasome (containing both alpha- and beta-subunits). despite the significant primary sequence divergence between m. smegmatis lon (ms-lon) and e. coli lon (ec-lon), expression of ms-lon was only moderately ... | 1998 | 9425059 |
| multiple forms of dna polymerase from the thermo-acidophilic eubacterium bacillus acidocaldarius: purification, biochemical characterization and possible biological role. | two dna polymerase isoenzymes, called dpa and dpb on the basis of their elution order from deae cellulose, were purified to homogeneity from the thermo-acidophilic eubacterium bacillus acidocaldarius. the enzymes are weakly acidophilic proteins constituted by a single subunit of 117 and 103 kda respectively. dpa and dpb differ in thermostability, in thermophilicity, in sensitivity to assay conditions and in resistance to sulphydryl-group blocking agents such as n-ethylmaleimide and p-hydroxymerc ... | 1998 | 9425113 |
| selective bowel decontamination of recipients for prevention against liver injury following orthotopic liver transplantation: evaluation with rat models. | gut-derived substances can activate kupffer cells to provoke hepatic necrosis after partial hepatectomy in rats. a similar situation may occur during orthotopic liver transplantation (olt), as congestion in the intestinal wall, caused by portal vein occlusion, is inevitable during the operation. the contribution of such substances to liver injury following olt was investigated in rats. oral administration of polymyxin b sulfate for 7 days significantly altered intestinal bacterial flora in rats; ... | 1998 | 9425927 |
| nitrate-reducing bacteria in diversion colitis: a clue to inflammation? | a pathogenic role of nitric oxide has been suggested in acute and chronic intestinal inflammation. we took the opportunity offered by studies in patients with excluded colon, which represents a model of chronic intestinal inflammation with no exogenous nitrite or nitrate supply, to evaluate the quantity and the quality of nitrate reducers in diversion colitis. thirty patients (17 men, 13 women, mean age 45 years) having an excluded colon for various reasons were sampled by rectal swabs and compa ... | 1997 | 9440640 |
| hydrolysis of glycyrrhetyl mono-glucuronide to glycyrrhetic acid by glycyrrhetyl mono-glucuronide beta-d-glucuronidase of eubacterium sp. glh. | glycyrrhetyl mono-glucuronide (gamg) is an intermediate in the hydrolysis of glycyrrhizin (gl) to glycyrrhetic acid (ga). an enzyme responsible for its hydrolysis, characterized as a gamg beta-d-glucuronidase of eubacterium sp. (species) glh, has been isolated from human intestinal bacteria. the pattern of gamg beta-d-glucuronidase activity was different from that of gl beta-d-glucuronidase activity by butyl-toyopearl 650 s column chromatography. thus, these enzymes showed differences in the pur ... | 1997 | 9448097 |
| enzymes responsible for the metabolism of saikosaponins from eubacterium sp. a-44, a human intestinal anaerobe. | from a human intestinal bacterium, eubacterium sp. a-44, which is capable of hydrolyzing saikosaponins to saikogenins, two glycosidases, beta-d-glucosidase and a novel type of beta-d-fucosidase, were isolated and characterized as saikosaponin-hydrolyzing beta-d-glucosidase and prosaikogenin-hydrolyzing beta-d-fucosidase. relative to the hydrolyzing activities toward saikosaponins a, b1 and b2, the beta-d-glucosidase showed lower ability to hydrolyze saikosaponin d, but no ability to hydrolyze sa ... | 1997 | 9448103 |
| the arthromitus stage of bacillus cereus: intestinal symbionts of animals. | in the guts of more than 25 species of arthropods we observed filaments containing refractile inclusions previously discovered and named "arthromitus" in 1849 by joseph leidy [leidy, j. (1849) proc. acad. nat. sci. philadelphia 4, 225-233]. we cultivated these microbes from boiled intestines of 10 different species of surface-cleaned soil insects and isopod crustaceans. literature review and these observations lead us to conclude that arthromitus are spore-forming, variably motile, cultivable ba ... | 1998 | 9448315 |
| the first isolation of eubacterium suis in hungary. | eubacterium suis was isolated from the preputium of seven out of 16 mature boars on two farms and from the urinary bladder of one out of five sows originating from a third herd. the morphological and biochemical characteristics of the isolated strains were identical to that of the reference strain of e. suis attc 33144. three out of four strains isolated from farm a were successfully subcultured aerobically, and then anaerobically again. e. suis together with proteus mirabilis was isolated from ... | 1997 | 9451944 |
| evaluation of root canal bacteria and their antimicrobial susceptibility in teeth with necrotic pulp. | this study aimed to evaluate the microbiota of necrotic pulp in teeth without carious lesions where the crown and root were intact and to test the sensitivity of this microbiota to antibiotics in order to improve treatment. the necrotic pulp was sampled from 26 single-rooted teeth in intact pulp chambers. a total of 84 strains were isolated. the number of species isolated per tooth varied from 2 to 8, with a strong component (81%) of anaerobic bacteria. the most commonly represented species were ... | 1997 | 9467386 |
| occurrence of peptidyl d-amino acids in soluble fractions of several eubacteria, archaea and eukaryotes. | the occurrence of peptidyl d-amino acids in the aqueous soluble fractions was investigated in various eubacteria, some archaea and some eukaryotes. the contents of the d-enantiomers of serine, alanine, proline, glutamate (glutamine), aspartate (asparagine) and phenylalanine were determined with cell- and tissue-extracts, by means of acid hydrolysis and high-performance liquid chromatography. the rate of d-enantiomer (%, the ratio in molar concentration of a d-amino acid to the total of the d-ami ... | 1998 | 9468335 |
| conjugal transfer of transposon tn1545 into the cellulolytic bacterium eubacterium cellulosolvens. | tn1545, a self-mobilizing transposon, was introduced into the chromosome of the ruminal cellulolytic bacterium eubacterium cellulosolvens. this was achieved by conjugal transfer of the transposon from clostridium beijerinckii at a frequency of 1 per 10(6) recipient cells. transconjugants of eu. cellulosolvens were resistant to both tetracycline and erythromycin, and were able to mobilize tn1545 back into cl. beijerinckii. southern blot hybridization of representative transconjugants did not reve ... | 1998 | 9489031 |
| microbial complexes in subgingival plaque. | it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ... | 1998 | 9495612 |
| clinical and microbiological features of refractory periodontitis subjects. | the purpose of this investigation was to compare the clinical parameters and the site prevalence and levels of 40 subgingival species in successfully treated and refractory periodontitis subjects. 94 subjects received scaling and root planing and if needed, periodontal surgery and systemically administered tetracycline. 28 refractory subjects showed mean full mouth attachment loss and/or > 3 sites showing attachment loss > 2.5 mm within 1 year post-therapy. 66 successfully treated subjects showe ... | 1998 | 9495617 |
| cellular fatty acid composition, soluble-protein profile, and antimicrobial resistance pattern of eubacterium lentum. | phenotypic heterogeneity among isolates of eubacterium lentum has been recognized for many years. to better delineate their taxonomic relatedness, 29 clinical isolates of e. lentum were examined for soluble-protein content, cellular fatty acid profile, and antimicrobial resistance pattern in order to ascertain whether differences in these characteristics could be correlated with differences in biochemical activities. among 29 isolates we could identify 6 that were different from all the others. ... | 1998 | 9508307 |
| the hydrogen hypothesis for the first eukaryote. | a new hypothesis for the origin of eukaryotic cells is proposed, based on the comparative biochemistry of energy metabolism. eukaryotes are suggested to have arisen through symbiotic association of an anaerobic, strictly hydrogen-dependent, strictly autotrophic archaebacterium (the host) with a eubacterium (the symbiont) that was able to respire, but generated molecular hydrogen as a waste product of anaerobic heterotrophic metabolism. the host's dependence upon molecular hydrogen produced by th ... | 1998 | 9510246 |
| tethered cord associated with intraspinal lipoma and a subcutaneous abscess secondary to a dermal sinus. | | 1998 | 9522249 |
| cloning, sequence analysis and expression in e. coli of the dna polymerase i gene from chloroflexus aurantiacus, a green nonsulfur eubacterium. | we have cloned and sequenced the pola gene from chloroflexus aurantiacus, a green nonsulfur eubacterium, and expressed the recombinant protein in escherichia coli. one open reading frame encodes a protein with 942 amino acids showing 38% identity with dna polymerase i from e. coli. sequence alignments with other members of dna polymerase family a and analysis of the separate domains show that the central 3'-5' exonuclease domain is 30% identical to the corresponding e. coli domain and that three ... | 1998 | 9526698 |
| antibiotic therapy and serum digoxin toxicity. | | 1998 | 9531908 |
| the deconvolution of pyrolysis mass spectra using genetic programming: application to the identification of some eubacterium species. | pyrolysis mass spectrometry was used to produce complex biochemical fingerprints of eubacterium exiguum, e. infirmum, e. tardum and e. timidum. to examine the relationship between these organisms the spectra were clustered by canonical variates analysis, and four clusters, one for each species, were observed. in an earlier study we trained artificial neural networks to identify these clinical isolates successfully; however, the information used by the neural network was not accessible from this ... | 1998 | 9532743 |
| fast purification of thioredoxin reductases and of thioredoxins with an unusual redox-active centre from anaerobic, amino-acid-utilizing bacteria. | thioredoxin reductase and thioredoxin are primarily involved in catabolic metabolism as important electron carriers in anaerobic, amino-acid-degrading bacteria. a general and fast procedure was developed for the purification of thioredoxin reductase and thioredoxin from eubacterium acidaminophilum, clostridium litorale, c. sticklandii, c. sporogenes, c. cylindrosporum and 'tissierella creatinophila' based upon their properties: the binding to 2',5'-amp-sepharose by thioredoxin reductase and the ... | 1998 | 9534247 |
| omeprazole induces altered bile acid metabolism. | it has been reported that the acidity of gastric contents could be an important factor in regulating jejunal flora. | 1998 | 9536953 |
| the intriguing evolution of the "b" and "g" subunits in f-type and v-type atpases: isolation of the vma-10 gene from neurospora crassa. | we have characterized the vma-10 gene which encodes the g subunit of the vacuolar atpase in neurospora crassa. the gene is somewhat unusual in filamentous fungi because it contains five introns, comprising 71% of the region between the translation start and stop codons. the 5' untranslated region of the gene contains several elements that have been identified in other genes that encode subunits of the vacuolar atpase in n. crassa. a comparison of g subunits from n. crassa, s. cerevisiae, and ani ... | 1997 | 9559854 |
| construction and characterization of an nacl-sensitive mutant of halomonas elongata impaired in ectoine biosynthesis. | using transposon mutagenesis we generated a salt-sensitive mutant of the halophilic eubacterium halomonas elongata impaired in the biosynthesis of the compatible solute ectoine. hplc determinations of the cytoplasmic solute content showed the accumulation of a biosynthetic precursor of ectoine, l-2,4-diaminobutyric acid. ectoine and hydroxyectoine were not detectable. this mutant failed to grow in minimal medium with nacl concentrations exceeding 4%. however, when supplemented with organic osmol ... | 1998 | 9570121 |
| ammonia-hyperproducing bacteria from new zealand ruminants. | pasture-grazed dairy cows, deer, and sheep were tested for the presence of ammonia-hyperproducing (hap) bacteria in roll tubes containing a medium in which tryptone and casamino acids were the sole nitrogen and energy sources. colonies able to grow on this medium represented 5.2, 1.3, and 11.6% of the total bacterial counts of dairy cows, deer, and sheep, respectively. a total of 14 morphologically distinct colonies were purified and studied further. restriction fragment length polymorphisms of ... | 1998 | 9572953 |
| subgingival microbiota of shallow periodontal pockets in individuals after head and neck irradiation. | this study aimed at investigating the subgingival plaque microorganisms of shallow pockets (< or = 5 mm) in subjects who previously received irradiation in the head and neck region for treatment of nasopharyngeal carcinoma. direct microscopy and anaerobic culture were used. subgingival paper point samples were taken from 6 tooth-sites (one/sextant) per subject for direct microscopy (n = 108). another set of paper points was taken from the deepest of the previously selected sites (one per subject ... | 1998 | 9573815 |
| restriction fragment-length polymorphism analysis of 16s rdna from oral asaccharolytic eubacterium species amplified by polymerase chain reaction. | restriction fragment-length polymorphism (rflp) analysis of 16s rdna amplified by polymerase chain reaction was used to generate restriction profiles of the type strains of oral asaccharolytic eubacterium species, that is, eubacterium brachy, eubacterium exiguum, eubacterium lentum, eubacterium minutum, eubacterium nodatum, eubacterium saphenum, eubacterium timidum and 33 asaccharolytic eubacterium strains isolated from oral sites. the 16s rrna gene sequences from isolated genomic dna samples we ... | 1998 | 9573818 |
| overexpression and properties of a new thermophilic and thermostable esterase from bacillus acidocaldarius with sequence similarity to hormone-sensitive lipase subfamily. | we previously purified a new esterase from the thermoacidophilic eubacterium bacillus acidocaldarius whose n-terminal sequence corresponds to an open reading frame (orf3) reported to show homology with the mammalian hormone-sensitive lipase (hsl)-like group of the esterase/lipase family. to compare the biochemical properties of this thermophilic enzyme with those of the homologous mesophilic and psychrophilic members of the hsl group, an overexpression system in escherichia coli was established. ... | 1998 | 9576869 |
| citrate synthase and 2-methylcitrate synthase: structural, functional and evolutionary relationships. | following the complete sequencing of the escherichia coli genome, it has been shown that the proposed second citrate synthase of this organism, recently described by the authors, is in fact a 2-methylcitrate synthase that possesses citrate synthase activity as a minor component. whereas the hexameric citrate synthase is constitutively produced, the 2-methylcitrate synthase is induced during growth on propionate, and the catabolism of propionate to succinate and pyruvate via 2-methylcitrate is pr ... | 1998 | 9579066 |
| cloning, sequencing and overexpression of a rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12. | a gene library from the thermophilic eubacterium rhodothermus marinus, strain iti 378, was constructed in puc18 and transformed into escherichia coli. of 5400 transformants, 3 were active on carboxymethylcellulose. three plasmids conferring cellulase activity were purified and were all found to contain the same cellulase gene, cela. the open reading frame for the cela gene is 780 base pairs and encodes a protein of 260 amino acids with a calculated molecular mass of 28.8 kda. the amino acid sequ ... | 1998 | 9581291 |
| [endocarditis caused by eubacterium spp]. | | 1998 | 9586426 |