ultrasound enhanced growth and cholesterol removal of lactobacillus fermentum ftdc 1311 in the parent cells but not the subsequent passages. | the aim of this study was to evaluate the effect of ultrasound on the intestinal adherence ability, cell growth, and cholesterol removal ability of parent cells and subsequent passages of lactobacillus fermentum ftdc 1311. ultrasound significantly decreased the intestinal adherence ability of treated parent cells compared to that of the control by 11.32% (p<0.05), which may be due to the protein denaturation upon local heating. growth of treated parent cells also decreased by 4.45% (p<0.05) imme ... | 2012 | 22265020 |
synergistic effect between two bacteriocin-like inhibitory substances produced by lactobacilli strains with inhibitory activity for streptococcus agalactiae. | group b streptococci (gbs) are bacterial species that colonize the vagina in pregnant women and as such may cause serious infections in neonates that passed through the birth channel. the objective of this work was to study the inhibitory activities produced by each bacteriocin-like inhibitory substance (blis) of lactobacillus rhamnosus l60 and lactobacillus fermentum l23, and the effects of the combined blis-es of these lactobacilli on gbs. the interactions between the blis-es were assessed by ... | 2012 | 22231454 |
probiotic ferulic acid esterase active lactobacillus fermentum ncimb 5221 apa microcapsules for oral delivery: preparation and in vitro characterization. | probiotics possess potential therapeutic and preventative effects for various diseases and metabolic disorders. one important limitation for the oral delivery of probiotics is the harsh conditions of the upper gastrointestinal tract (git) which challenge bacterial viability and activity. one proposed method to surpass this obstacle is the use of microencapsulation to improve the delivery of bacterial cells to the lower git. the aim of this study is to use alginate-poly-l-lysine-alginate (apa) mi ... | 2012 | 24288090 |
isolation, characterization and identification of a potential probiont from south indian fermented foods (kallappam, koozh and mor kuzhambu) and its use as biopreservative. | twenty-five strains of lactic acid bacteria (lab) isolated from south indian traditional fermented foods kallappam batter, koozh and mor kuzhambu. further 6 strains were selected based on their antimicrobial activity. they were identified according to morphological, biochemical and physiological criteria. identification by 16s rdna sequence homology of the isolates revealed the presence of weissella paramesenteroides, lactobacillus plantarum and lactobacillus fermentum. lactobacillus plantarum a ... | 2010 | 26781237 |
antibacterial and antioxidant activities of acid and bile resistant strains of lactobacillus fermentum isolated from miang. | miang is a kind of traditional fermented tea leaves, widely consumed in northern thailand as a snack. it contains several kinds of lactobacilli spp. the aim of this study was to isolate strains of lactobacillus fermentum from miang and to investigate their antibacterial and antioxidant activities. the agar spot and well assays were used for determination of antibacterial power. the antibacterial mechanism was investigated by cell morphologic change under scanning electron microscope (sem). antio ... | 2009 | 24031422 |
experiences with the use of a starter culture in the fermentation of maize for 'kenkey' production in ghana. | controlled fermentation of maize was carried out using six strains of lactobacillus fermentum and one strain of yeast, saccharomyces cerevisiae, isolated from traditionally fermented maize dough as starter cultures for inoculum enrichement. the fermentations were monitored by ph, acidity, microbiological analysis and taste panel evaluation of two products, kenkey and koko, prepared from the fermented doughs. the strains of l. fermentum used as starter culture dominated the microflora during ferm ... | 1996 | 24415385 |
chemical modifications of the cell-surface components of lactobacillus fermentum ftpt 1405 and their effect on the flocculation of saccharomyces cerevisiae. | the effect of treatment of lactobacillus fermentum with several protein- and carbohydrate-modifying reagents on the bacterium's ability to flocculate saccharomyces cerevisiae was investigated. the proteinaceous nature of the cell-surface components of l. fermentum which are responsible for floc formation was confirmed by inactivation of floc formation following photo-irradiation, with methylene blue or rose bengal as sensitizer, or acylation with acetic anhydride, maleic anhydride or acetylimida ... | 1995 | 24414903 |
evaluation of bacterial contamination in a fed-batch alcoholic fermentation process. | alcoholic fermentation by a commercial baker's yeast in a fed-batch process with cell recycling and high-test molasses as substrate was strongly inhibited by lactobacillus fermentum cct 1407 after a few recycles. when total acidity (mainly lactic acid) exceeded 4.8 g/l broth it seriously interfered with yeast bud formation and viability and above 6.0 g/l it decreased alcoholic efficiency. | 1994 | 24421199 |
microbiological and biochemical changes during the fermentation of maize (zea mays) grains formasa production. | microorganisms associated with fermentation ofmasa werelactobacillus plantarum, pediococcus acidilactici, lactobacillus fermentum andsaccharomyces cerevisiae. during the fermentation of maize grains, the temperature and moisture increased and the ph decreased. most amino acids decreased in concentration during fermentation, with glutamic acid being the most abundant amino acid, but he protein content of the 'masa' increased during fermentation. the bacteria played the major role in the fermentat ... | 1990 | 24429990 |
antimicrobial and anti-biofilm effect of bac8c on major bacteria associated with dental caries and streptococcus mutans biofilms. | dental caries is a common oral bacterial infectious disease. its prevention and treatment requires control of the causative pathogens within dental plaque, especially streptococcus mutans (s. mutans). antimicrobial peptides (amps), one of the promising substitutes for conventional antibiotics, have been widely tested and used for controlling bacterial infections. the present study focuses on evaluating the potential of the novel amps cyclic bactenecin and its derivatives against bacteria associa ... | 2014 | 24309076 |
antagonistic and quantitative assessment of indigenous lactic acid bacteria in different varieties of ogi against gastrointestinal pathogens. | ogi is a popular fermented cereal gruel consumed mainly in the western part of nigeria. traditionally, uncooked ogi is normally administered to diarrhoea patients to reduce the frequency of stooling. this study was therefore undertaken to identify, quantify and determine the antimicrobial properties of lactic acid bacteria (lab) isolated from ogi. | 2017 | 28748023 |
in vitro and in vivo antagonistic activity of new probiotic culture against clostridium difficile and clostridium perfringens. | genus clostridium accompanies more than 200 known species and at least 30 among them are associated with human and animal diseases. at the moment, the treatment of clostridial infections is based on use of antibiotics. however, due to the european ban on the use of antibiotics in livestock production, novel therapeutic strategies for treatment of these hardly curable infections have been evaluated. hence, in this study the antimicrobial effect of newly designed probiotic culture consisted of nat ... | 2017 | 28477627 |
assessment of phenotypic and genotypic antibiotic susceptibility of vaginal lactobacillus sp. | to assess antibiotic susceptibility of vaginal lactobacilli strains and provide the data required for assessing the potential of antibiotic resistance risk of new strains selected as probiotic. | 2017 | 28574626 |
isolation of antifungal lactic acid bacteria (lab) from "kunu" against toxigenic aspergillus flavus. | the antifungal activity of isolated lactic acid bacteria (lab) from a locally fermented cereal, "kunu", was tested against toxigenic aspergillus flavus. the liquid refreshment, "kunu", was prepared under hygienic condition using millet, sorghum, and the combination of the two grains. the antifungal potential of isolated lab against toxigenic a. flavus was carried out using both in vitro and in vivo antifungal assays. the lab count from prepared "kunu" ranged from 2.80 ×10(4) cfu/ml to 4.10×10(4) ... | 2017 | 28702431 |
microbial interactions for enhancement of α-amylase production by bacillus amyloliquefaciens 04bba15 and lactobacillus fermentum 04bba19. | interactions occurring between saccharomyces cerevisiae and two thermostable α-amylase producing strains (bacillus amyloliquefaciens 04bba15 and lactobacillus fermentum 04bba19) were analyzed by comparing their growth patterns obtained in isolation with those obtained in mixture. the difference between the patterns was assessed using analysis of variance (anova) in order to measure how much the growth of an organism was affected by other. the results showed two types of interactions in mixed cul ... | 2014 | 28626668 |
diversity and probiotic potential of lactic acid bacteria isolated from horreh, a traditional iranian fermented food. | the aim of this study was to evaluate the probiotic potential of lactic acid bacteria (lab) strains isolated from horreh. some probiotic properties, e.g., resistance to acid, bile tolerance, antibacterial activity, and antibiotic susceptibility, were investigated. a total of 140 gram-positive and catalase-negative isolates from horreh were subjected to identification and grouping by cultural methods and the 16s rrna sequencing. the new isolates were identified to be lactobacillus (fermentum, pla ... | 2017 | 28527125 |
longterm storage of post-packaged bread by controlling spoilage pathogens using lactobacillus fermentum c14 isolated from homemade curd. | one potent lactic acid bacterial strain c14 with strong antifungal activity was isolated from homemade curd. based on morphological as well as biochemical characters and 16s rdna sequence homology the strain was identified as lactobacillus fermentum. it displayed a wide antimicrobial spectrum against both gram-positive and gram-negative pathogenic bacteria, and also against number of food spoilage, plant and human pathogenic fungi. the cell free supernatant (cfs) of the strain c14 was also effec ... | 2017 | 28859156 |
lactobacillus fermentum 3872 as a potential tool for combatting campylobacter jejuni infections. | due to the global spread of multidrug resistant pathogenic bacteria, alternative approaches in combating infectious diseases are required. one such approach is the use of probiotics. lactobacillus fermentum 3872 is a promising probiotic bacterium producing a range of antimicrobial compounds, such as hydrogen peroxide and lactic acid. in addition, previous studies involving genome sequencing and analysis of l. fermentum 3872 allowed the identification of a gene encoding a cell surface protein ref ... | 2017 | 28766992 |
in vitro characterization of lactobacillus strains isolated from fruit processing by-products as potential probiotics. | nine wild lactobacillus strains, namely lactobacillus plantarum 53, lactobacillus fermentum 56, l. fermentum 60, lactobacillus paracasei 106, l. fermentum 250, l. fermentum 263, l. fermentum 139, l. fermentum 141, and l. fermentum 296, isolated from fruit processing by-products were evaluated in vitro for a series of safety, physiological functionality, and technological properties that could enable their use as probiotics. considering the safety aspects, the resistance to antibiotics varied amo ... | 2017 | 28836171 |
indigenous lactobacilli strains of food and human sources reverse enteropathogenic e. coli o26:h11-induced damage in intestinal epithelial cell lines: effect on redistribution of tight junction proteins. | the aim of the study was to investigate the neutralizing effect of lactobacilli isolated from indigenous food and human sources on enteropathogenic escherichia coli (epec) o26 : h11-induced epithelial barrier dysfunction in vitro. this was assessed by transepithelial electrical resistance (teer) and permeability assays using intestinal cell lines, ht-29 and caco-2. furthermore, the expression and distribution of tight junction (tj) proteins were analysed by qrt-pcr and immunofluorescence assay, ... | 2017 | 28771130 |
a non-endoscopic device to sample the oesophageal microbiota: a case-control study. | the strongest risk factor for oesophageal adenocarcinoma is reflux disease, and the rising incidence of this coincides with the eradication of helicobacter pylori, both of which might alter the oesophageal microbiota. we aimed to profile the microbiota at different stages of barrett's carcinogenesis and investigate the cytosponge as a minimally invasive tool for sampling the oesophageal microbiota. | 2017 | 28404012 |
comparing the impact of ultrafine particles from petrodiesel and biodiesel combustion to bacterial metabolism by targeted hplc-ms/ms metabolic profiling. | alterations of gut bacterial metabolism play an important role in their host metabolism, and can result in diseases such as obesity and diabetes. while many factors were discovered influencing the gut bacterial metabolism, exposure to ultrafine particles (ufps) from engine combustions were recently proposed to be a potential risk factor for the perturbation of gut bacterial metabolism, and consequentially to obesity and diabetes development. this study focused on evaluation of how ufps from dies ... | 2017 | 28410499 |
selection of new lactic acid bacteria strains bearing probiotic features from mucosal microbiota of healthy calves: looking for immunobiotics through in vitro and in vivo approaches for immunoprophylaxis applications. | from the birth, since their mucosal microbiota and immune system are not fully developed, newborn calves are susceptible to several mucosal pathogenic microorganisms. operating through humoral and non-humoral mechanisms in the host, several lactic acid bacteria strains bearing probiotic features are often employed in livestock as food supplement, improving animal production performance, promoting health and reducing the severity of mucosal infections. accordingly, we isolated, species-level iden ... | 2017 | 28527759 |
draft genome sequence of lactobacillus fermentum bfe 6620, a potential starter culture for african vegetable foods, isolated from fermented cassava. | we report the draft genome sequence of lactobacillus fermentum bfe 6620 from fermented cassava used as a potential starter culture for african vegetable fermentation. sequence analysis showed the assembled genome size to be 1,982,893 bp, encoding a predicted total of 2,003 protein-coding genes, 14 rrnas, 54 trnas, and 3 noncoding rnas (ncrnas). | 2017 | 28818901 |
functional profile evaluation of lactobacillus fermentum tcuesc01: a new potential probiotic strain isolated during cocoa fermentation. | the use of intestinal probiotic bacteria is very common in the food industry and has been the focus of the majority of research in this field. yet in recent years, research on extraintestinal microorganisms has greatly increased due to their well-known potential as probiotics. thus, we studied a strain of lactobacillus fermentum (tcuesc01) extracted from fermenting cocoa. first, we examined the impact of ph on the growth of this strain and studied its survival under conditions similar to those o ... | 2017 | 28808659 |
label-free quantitative proteomic analysis of lactobacillus fermentum ncdc 400 during bile salt exposure. | lactobacillus fermentum ncdc 400 is a commonly used and a comprehensively studied probiotic organism. the distinctive capability to endure the harsh environment of the gastrointestinal tract owing to the presence of bile salts in gastric juice facilitates gut microbiota, especially the probiotic organisms to transiently inhabit the host gut. in the present study, label-free quantification (lfq) approach has been employed to analyze the expression pattern of lactobacillus fermentum ncdc 400 strai ... | 2017 | 28802582 |
differential intestinal anti-inflammatory effects of lactobacillus fermentum and lactobacillus salivarius in dss mouse colitis: impact on micrornas expression and microbiota composition. | to compare the intestinal anti-inflammatory effects of two probiotics lactobacillus fermentum and lactobacillus salivarius in mouse colitis, focusing on their impact on selected mirnas and microbiota composition. | 2017 | 28752563 |
health benefits observed after probiotic lactobacillus fermentum ccm 7421 application in dogs. | the importance of the intestinal microbiota has attracted much interest in recent years particularly with respect to ways in which the microbiota can be manipulated in order to improve health. improving gut health through the use of probiotic microorganisms has become an area of research activity in both human and animal nutrition. however, the amount of research using companion animals is insufficient. the present review evaluates and compares the effects achieved after application of canine-de ... | 2017 | 28721546 |
prevent effects of lactobacillus fermentum hy01 on dextran sulfate sodium-induced colitis in mice. | the aim of this study is to assess the preventive effects of lactobacillus fermentum hy01 (lf-hy01) to dextran sulfate sodium induced-colitis. we observed the ratio of colon weight to its length, colon pathological changes, and the concentrations of pro-inflammatory factors (ifn-γ, il-12, tnfα, and il-6) in serum. we also took account of the protein levels of iκbα, nf-κb p65, inos, and cox-2, and we measured the best effects of different doses of lactobacillus fermentum hy01 (low dose group was ... | 2017 | 28587089 |
in silico prediction, in vitro antibacterial spectrum, and physicochemical properties of a putative bacteriocin produced by lactobacillus rhamnosus strain l156.4. | a bacteriocinogenic lactobacillus rhamnosus l156.4 strain isolated from the feces of nih mice was identified by 16s rrna gene sequencing and maldi-tof mass spectrometry. the entire genome was sequenced using illumina, annotated in the pgaap, and rast servers, and deposited. conserved genes associated with bacteriocin synthesis were predicted using bagel3, leading to the identification of an open reading frame (orf) that shows homology with the l. rhamnosus gg (atcc 53103) prebacteriocin gene. th ... | 2017 | 28579977 |
production of organic flavor compounds by dominant lactic acid bacteria and yeasts from obushera, a traditional sorghum malt fermented beverage. | single and mixed starter cultures of lactic acid bacteria (lab): weissella confusa mnc20, lactobacillus plantarum mnc21, lactococcus lactis mnc24 and lactobacillus fermentum mnc34 and yeasts: issatchenkia orientalis mnc20y and saccharomyces cerevisiae mnc21y were used to produce obushera, a fermented sorghum beverage. microbial counts, ph, sugars, organic acids, and volatile compounds in starter culture and spontaneous fermentations were monitored during 48 hrs. maximum counts of lab (8.4-9.4 lo ... | 2017 | 28572960 |
study protocol: evaluation of the probiotic lactobacillus fermentum cect5716 for the prevention of mastitis in breastfeeding women: a randomised controlled trial. | mastitis and accompanying pain have been associated with the cessation of breastfeeding. mastitis is an inflammatory condition of the breast and may be a result of decreased immunity and a lowered resistance to infection. mastitis affects up to one in five breastfeeding women with most episodes occurring in the first 6-8 weeks postpartum. antibiotics are often used in the treatment of mastitis, but have not been popular or proven effective as a preventative agent. the who has highlighted signifi ... | 2017 | 28525984 |
lactobacillus fermentum im12 attenuates inflammation in mice by inhibiting nf-κb-stat3 signalling pathway. | in the present study, we isolated lactobacillus fermentum im12 from human gut microbiota, which strongly inhibited interleukin (il)-6 expression and stat3 activation in lipopolysaccharide (lps)-stimulated murine peritoneal macrophages, and examined its anti-inflammatory effect in mice with carrageenan-induced hind-paw oedema (cie) or 2,4,6-trinitrobenzene sulfonic acid (tnbs)-induced colitis (tic). oral administration of im12 (0.2×10(9), 1×10(9) or 5×10(9) cfu/mouse, once a day for 3 days) in mi ... | 2017 | 28504577 |
antioxidant activity of various oral lactobacillus strains. | this study aims to evaluate the antioxidant activity of oral lactobacillus strains in vitro. | 2017 | 28474851 |
multilocus genetic characterization of lactobacillus fermentum isolated from ready-to-eat canned food. | the primary mission of the u.s. food and drug administration is to enforce the food, drug, and cosmetic act and regulate food, drug, and cosmetic products. thus, this agency monitors the presence of pathogenic microorganisms in these products, including canned foods, as one of the regulatory action criteria and also ensures that these products are safe for human consumption. this study was carried out to investigate the effectiveness of pathogen control and integrity of ready-to-eat canned food ... | 2017 | 28467184 |
a synbiotic composed of lactobacillus fermentum cect5716 and fos prevents the development of fatty acid liver and glycemic alterations in rats fed a high fructose diet associated with changes in the microbiota. | we investigated the effect of a high fructose diet (hfd) on sprague dawley rats and the impact of a synbiotic composed of lactobacillus fermentum cect5716 and fructooligosaccharides. feeding the hfd for 5 weeks resulted in liver steatosis and insulin resistance but not obesity. these changes were associated with increased production of short-chain fatty acids and increased bacteroidetes in feces, with an augmented bacteroidetes/firmicutes ratio, among other changes in the microbiota. in addition ... | 2017 | 28463404 |
development and application of anticancer fluorescent cds nanoparticles enriched lactobacillus bacteria as therapeutic microbots for human breast carcinoma. | applications of probiotic bacteria and nanoparticles (nps) as therapeutic agents have great importance. this study demonstrates a combinatorial approach of both the probiotic lactobacillus spp. (lactobacillus fermentum and lactobacillus plantarum) with fluorescent cadmium sulfide (cds) nps as therapeutic agents to target mcf-7 cancer cells (human breast cancer cells). in this study, facultative anaerobic lactobacillus was successfully used as a vehicle to transport nps into mcf-7 cancer cells. t ... | 2017 | 28455616 |
genome characteristics of lactobacillus fermentum strain jdfm216 for application as probiotic bacteria. | lactobacillus fermentum strain jdfm216, isolated from a korean infant feces sample, possesses the ability to enhance the longevity and immune response of a caenorhabditis elegans host. to explore the characteristics of strain jdfm216 at the genetic level, we performed whole-genome sequencing using the pacbio system. the circular draft genome has a total length of 2,076,427 bp and a total of 2,682 encoding sequences were identified. five phylogenetically featured genes possibly related to the lon ... | 2017 | 28438015 |
lactobacillus fermentum postbiotic-induced autophagy as potential approach for treatment of acetaminophen hepatotoxicity. | the aim of this study was to investigate the potential of postbiotics originated from lactobacillus fermentum bghv110 strain (hv110) to counteract acetaminophen (apap)-induced hepatotoxicity in hepg2 cells. this strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in apap caused cellular damage. cell viability was assessed using mtt and ldh assays, while autophagy was monitored by qpcr analysis of becn1, atg5, p62/sqs ... | 2017 | 28428777 |
study on the chemical and microbial composition and probiotic characteristics of dominant lactic acid bacteria in fermented poultry slaughterhouse waste. | this study aimed to evaluate the quality of fermented poultry slaughterhouse waste and isolate and identify the probiotic bacteria present in it. poultry slaughterhouse waste samples supplemented with 5% starter culture and 0.2% propionic acid received one of four experimental treatments, which consisted of further supplementation with: (1) 10% molasses (m10); (2) 15% molasses (m15); (3) 10% molasses with 200ppm butylated hydroxytoluene (mb10); and (4) 15% molasses with 200ppm butylated hydroxyt ... | 2017 | 28408278 |
effects of trang phuc linh plus-food supplement on irritable bowel syndrome induced by mustard oil. | trang phuc linh plus (tplp) is a food supplement product derived from dried extracts of herbal agents atractylodes macrocephala, poria cocos, paeonia lactiflora, phellodendron amurense, and added lactobacillus fermentum lysate (immunegamma(®)) and 5-hydroxytryptophan. tplp is a functional food used as adjunctive treatment for treating irritable bowel syndrome (ibs). however the biological effect and its mechanism of action in ibs have not been elucidated. in this study, we aimed to determine the ... | 2017 | 28406734 |
microbiological and chemical parameters during cassava based-substrate fermentation using potential starter cultures of lactic acid bacteria and yeast. | traditional brazilian indigenous fermented foods and beverages are potential sources of new food products that promote health, but they are still produced by natural fermentation. in the present work, lactobacillus fermentum ccma 0215 isolated from the indigenous fermented cassava beverage yakupa was used as single or mixed starter culture with five different yeast strains (torulaspora delbrueckii ccma 0234 and ccma 0235, pichia caribbica ccma 0198, and saccharomyces cerevisiae ccma 0232 and ccm ... | 2015 | 28455064 |