| the effect of sodium chloride on heat resistance and recovery of heated spores of bacillus stearothermophilus. | | 1969 | 5791939 |
| infection of bacillus stearothermophilus with bacteriophage deoxyribonucleic acid. | early stationary-phase cells are most susceptible to infection with deoxyribonucleic acid from bacteriophage tp-1c. transfection is destroyed by deoxyribonuclease and unaffected by phage antiserum. | 1969 | 5802614 |
| the binding of spermine to the ribosomes and ribosomal ribonucleic acid from bacillus stearothermophilus. | 1. the total intracellular concentrations of na(+), k(+), mg(2+), spermine, spermidine and rna were measured in bacillus stearothermophilus. 2. the binding of spermine to ribosomes and to ribosomal rna from b. stearothermophilus was studied under various conditions by using a gel-filtration technique. 3. the affinity of spermine for ribosomes and for ribosomal rna decreased with increasing ionic strength of the medium in which they were suspended. 4. the extent of spermine binding did not change ... | 1969 | 5806384 |
| prototrophic thermophilic bacillus: isolation, properties, and kinetics of growth. | a thermophilic bacillus (minimal growth temperature 41 c, optimal 55 to 58 c, and maximal 65 c) was isolated from a manure pile. it is very similar to bacillus stearothermophilus, but it differs in its inability to hydrolyze starch. the thermophilic isolate is a prototroph which grows in a minimal medium consisting of glucose, ammonium salt, phosphate buffer, and inorganic salts. at all temperatures studied (low to high), the same minimal nutritional requirements prevailed. the arrhenius constan ... | 1969 | 5808072 |
| purification and some properties of leucyl-trna synthetase from bacillus stearothermophilus. | | 1969 | 5823096 |
| immunochemical studies on alpha-amylase. 3. immunochemical relationships among amylases from various microorganisms. | sirishinha, stitaya (university of rochester school of medicine and dentistry, rochester, n.y.), and peter z. allen. immunochemical studies on alpha-amylase. iii. immunochemical relationships among amylases from various microorganisms. j. bacteriol. 90:1120-1128. 1965.-immunochemical relationships among amylases obtained from a selected group of microorganisms were examined, and a cross-reaction was detected between the alpha-amylases of bacillus stearothermophilus and b. subtilis. immunodiffusi ... | 1965 | 5847799 |
| biochemical changes in lysogenic bacillus stearothermophilus after bacteriophage induction. | welker, n. e. (university of illinois, urbana), and l. leon campbell. biochemical changes in lysogenic bacillus stearothermophilus after bacteriophage induction. j. bacteriol. 90:1129-1137. 1965.-cultures of bacillus stearothermophilus 1503-4r (tp-1) continued to grow at an unaltered rate after induction with mitomycin c (mc). mc-induced cultures exhibited a 2.5-fold increase in cell number before lysis occurred. prior to lysis, cells were observed to elongate and to contain areas of lesser dens ... | 1965 | 5847800 |
| relationship between heat activation and percentage colony formation for bacillus stearothermophilus spores: effects of storage and ph of the recovery medium. | | 1965 | 5852354 |
| effect of several environmental conditions on the "thermal death rate" of endospores of aerobic, thermophilic bacteria. | the composition of the recovery medium affected the apparent heat resistance of bacillus stearothermophilus when the ph of the medium was 7.0 but not when the ph was 6.5. the rate of thermal death at 110 c was exponential. deviations from exponential rates of thermal death during the initial phases of heating at 96 c were observed with endospores of b. coagulans under different conditions of sporulation. additionally, the apparent heat resistance was influenced by the composition of the media us ... | 1965 | 5866047 |
| isolation and preliminary characterization of bacteriophage phi-mu-4. | shafia, fred (university of nebraska, lincoln), and t. l. thompson. isolation and preliminary characterization of bacteriophage phimu-4. j. bacteriol. 87:999-1002. 1964.-bacteriophage phimu-4 was isolated from lysogenic bacillus stearothermophilus nu strain 4, and was propagated in strain 10 of the same species. the phage was extremely host-specific. one of the 23 strains of thermophiles screened for susceptibility supported phage replication. plaque-forming efficiency of phimu-4 depended on the ... | 1964 | 5874550 |
| ribonucleic acid and ribosomes of bacillus stearothermophilus. | saunders, grady f. (university of illinois, urbana), and l. leon campbell. ribonucleic acid and ribosomes of bacillus stearothermophilus. j. bacteriol. 91:332-339. 1966.-the ability of some thermophilic bacteria to grow at temperatures as high as 76 c emphasizes the remarkable thermal stability of their crucial macromolecules. an investigation of the ribonucleic acid (rna) and ribosomes of bacillus stearothermophilus was conducted. washed log-phase cells were disrupted either by sonic treatment ... | 1966 | 5903099 |
| characterization of a thermophilic bacteriophage for bacillus stearothermophilus. | saunders, grady f. (university of illinois, urbana), and l. leon campbell. characterization of a thermophilic bacteriophage for bacillus stearothermophilus. j. bacteriol. 91:340-348. 1965.-the biological and physical-chemical properties of the thermophilic bacteriophage tp-84 were investigated. tp-84 was shown to be lytic for 3 of 24 strains of bacillus stearothermophilus tested over the temperature range of 43 to 76 c. the latent period of tp-84 on b. stearothermophilus strain 10 was 22 to 24 m ... | 1966 | 5903101 |
| protein synthesis in a subcellular system from bacillus stearothermophilus. | | 1966 | 5914320 |
| isolation and identification of obligate thermophilic sporeforming bacilli from ocean basin cores. | bartholomew, j. w. (university of southern california, los angeles), and george paik. isolation and identification of obligate thermophilic sporeforming bacilli from ocean basin cores. j. bacteriol. 92:635-638. 1966.-obligate thermophilic sporeforming aerobic bacilli were isolated from 11 ocean basin cores taken from locations in a 150 mile long area off of the coast from ensenada, mexico, to santa catalina island, and ranging as far out from shore as 160 miles. isolated strains of bacilli were ... | 1966 | 5922538 |
| crystallization of thermostable glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. | | 1966 | 5969297 |
| amino acid uptake, protein and nucleic acid synthesis and turnover in bacillus stearothermophilus. | | 1966 | 5970344 |
| occurrence of isocitrate lyase in a thermophilic bacillus species. | a thermophilic, sporeforming bacterium has been isolated from soil on a medium containing acetate as a carbon source. this organism is similar to bacillus stearothermophilus in most respects but differs in its inability to hydrolyze starch. isocitrate lyase is present in cell-free extracts of organisms grown in a medium with acetate as a carbon source. the specific activity was 400 times lower in extracts of organisms utilizing glucose as a carbon source. with crude extracts, enzyme activity was ... | 1967 | 6020570 |
| stability of ribosomes and ribosomal ribonucleic acid from bacillus stearothermophilus. | after heating at 65 c, ribosomes isolated from bacillus stearothermophilus were strikingly more heat-stable than comparable preparations from escherichia coli when tested for ability to support polyuridylic acid-directed phenylalanine incorporation at 37 c. the stability of ribosomes was also determined by measurements of hyperchromicity at 259 mmu while heating them from 25 to 90 c. in standard buffer containing 0.01 m mg(++), the t(m) (temperature at the midpoint of total hyperchromicity) of e ... | 1967 | 6025440 |
| liquid medium for production of spores by bacillus stearothermophilus. | | 1967 | 6029843 |
| chemical composition of the cell walls of bacillus stearothermophilus. | cell walls were isolated by mechanical disruption of mid-log phase cells of bacillus stearothermophilus nca 1503-4r grown in trypticase-yeast extract-fructose medium at 55 c. the cell walls were purified by treatment with sodium dodecyl sulfate (sds) and incubation with deoxyribonuclease and trypsin. the cell wall peptidoglycan contained glucosamine, muramic acid, alpha, epsilon-diaminopimelic acid, and glutamic acid. low amounts of glycine, galactosamine, serine, aspartic acid, lysine, and vali ... | 1967 | 6032516 |
| pathways of glucose metabolism by rough and smooth variants of bacillus stearothermophilus. | the radiorespirometric method was used to study the pathways of glucose metabolism in the rough and smooth variants of bacillus stearothermophilus nca 1518. the embden-meyerhof (em) pathway was more active in the smooth variant than in the rough variant. the participation of the hexose monophosphate shunt (hmp) and em pathways in the smooth variant was calculated as 4.2 and 95.8%, respectively. the rough variant utilized glucose via the em pathway exclusively or in combination with a pathway oth ... | 1967 | 6035046 |
| factors from bacillus stearothermophilus that inhibit the binding of amino acids to transfer rna. | | 1967 | 6048285 |
| sporulation of bacillus stearothermophilus. | a broth medium containing tryptone and manganese sulfate supported heavy sporulation of bacillus stearothermophilus atcc 7953 (nca 1518) and four isolates identified as b. stearothermophilus. maximal spore yields were obtained by use of inocula grown anaerobically in a medium containing glucose with aeration of sporulation medium via bubbling. after an extended stationary period, sporulation occurred concurrently with vegetative growth between 6 and 8 hr of incubation at 60 c. omission of glucos ... | 1967 | 6053174 |
| partial purification and properties of a peptidase fraction from bacillus stearothermophilus. | | 1967 | 6062862 |
| extraction and characterization of deoxyribonucleic acid from spores and vegetive cells of bacillus stearothermophilus. | | 1967 | 6066059 |
| thermal inactivation characteristics of bacterial spores at ultrahigh temperatures. | the thermal inactivation characteristics of bacillus stearothermophilus (1518) spores and putrefactive anaerobe (pa) 3679 (nca) spores suspended in skim milk were determined after treatment in pilot-plant ultrahigh-temperature (uht) processing equipment. temperature-survivor curves were constructed from survival data to emphasize the critical nature of temperature control in process evaluation. time-survivor curves for pa 3679 spores were concave upward, and decimal reduction time (drt) curves f ... | 1967 | 6071918 |
| location of protein s4 on the small ribosomal subunit of e. coli and b. stearothermophilus with protein- and hapten-specific antibodies. | in spite of considerable effort there is still serious disagreement in the literature about the question of whether epitopes of ribosomal protein s4 are accessible for antibody binding on the intact small ribosomal subunit. we have attempted to resolve this issue using three independent approaches: (i) a re-investigation of the exposure and the location of epitopes of ribosomal protein s4 on the surface of the 30s subunit and 30s core particles of the e. coli ribosome, including rigorous control ... | 1984 | 6083434 |
| electron-paramagnetic-resonance studies of manganese(ii) complexes with elongation factor tu from bacillus stearothermophilus. observation of a gtp hydrolysis intermediate state complex. | changes in the coordination of mn2+ to nucleotide, water and protein at the active site of elongation factor tu (ef-tu) have been studied by electron paramagnetic resonance (epr) spectroscopy. from the time dependence of the mn2+ spectrum after addition of gtp to ef-tu x mn, it was apparent that three complexes with different epr linewidths could be detected. using additional information from the kinetics of 32pi production and release from ef-tu x mn x [gamma-32p]gtp these were identified as ef ... | 1984 | 6086334 |
| two replication determinants of an antibiotic-resistance plasmid, ptb19, from a thermophilic bacillus. | two different replication determinants were found on an antibiotic resistance plasmid, ptb19, from a thermophilic bacillus. one replication determinant (designated repa) was functional only in bacillus subtilis, whereas the other (designated repb) functioned in both b. subtilis and bacillus stearothermophilus. a deletion plasmid, ptb90, carrying the repb derived from ptb19 coincidentally contained the specific 1.0 mdal ecori fragment of a cryptic plasmid pbso2 from b. stearothermophilus. the pre ... | 1984 | 6090582 |
| continuous atp regeneration process with stable acetate kinase. | heat-stable acetate kinase (ak) from bacillus stearothermophilus was successfully immobilized covalently to sepharose resin by several conventional methods including carbodiimide, hydroxysuccinimide, cyanogen bromide, and glutaraldehyde and also by a new method which utilizes a bifunctional adp derivative as a spacer. the latter method gave a higher yield in terms of enzyme activity than the conventional methods. the properties and kinetics of the immobilized ak were studied batchwise and in a c ... | 1984 | 6092323 |
| nucleotide sequence of the triose phosphate isomerase gene of escherichia coli. | we report here the complete nucleotide sequence of the e. coli triose phosphate isomerase gene. the gene encodes a polypeptide of 255 amino acids which is approximately 46% homologous to eukaryotic triose phosphate isomerases, and approximately 38% homologous to the enzyme from a thermophilic bacterium, bacillus stearothermophilus. the nucleotide sequence is 55% homologous to that of the corresponding gene in the yeast saccharomyces cerevisiae. to our knowledge, this is the first report of the s ... | 1984 | 6092857 |
| complete nucleotide sequence of a 23s ribosomal rna gene from bacillus stearothermophilus. | a 23s ribosomal (rrna) gene from bacillus stearothermophilus has been cloned in pbr322, and its nucleotide sequence determined. the corresponding mature 23s rrna is predicted to contain 2928 nucleotides. we compare the primary and secondary structures of 23s rrna from escherichia coli and b. stearothermophilus, and discuss their potential contributions to thermal stability. | 1984 | 6096097 |
| spectroscopic studies of the nature of the iron clusters in the soluble hydrogenase from desulfovibrio desulfuricans (strain norway 4). | 57fe-enriched samples of the soluble hydrogenase from desulfovibrio desulfuricans (norway) have been investigated in both the native (oxidized) and the dithionite-reduced states using mössbauer spectroscopy. the data clearly show that the iron in this enzyme is predominantly in the form of iron-sulphur clusters which are closely similar to the [4fe-4s] clusters found in a large number of ferredoxins, such as that from bacillus stearothermophilus. there appear to be two [4fe-4s] clusters. the iro ... | 1984 | 6096146 |
| reversible acid dissociation of thermostable inorganic pyrophosphatase from bacillus stearothermophilus. | | 1980 | 6101557 |
| primary structure of triosephosphate isomerase from bacillus stearothermophilus. | 1. triosephosphate isomerase from bacillus stearothermophilus is a dimeric enzyme comprising two chemically identical polypeptide chains. 2. the nearly complete amino acid sequence of the subunit polypeptide chain has been established from sequences of tryptic, chymotryptic and lysine-blocked tyrptic fragments of s-[2-14c]carboxymethylated enzyme. overlaps not established by experimental data have been provisionally established from considerations of sequence homology with previously established ... | 1980 | 6105959 |
| evaluation of a prototype micro-electronic autoclave cycle integrator. | instability and non-uniformity of spore preparations and the non-conformity of chemical indicators to the temperature coefficients of spore inactivation are problems associated with current methods of autoclave cycle monitoring. a prototype micro-electronic instrument, which largely overcomes these problems, is described. it monitors autoclave cycles in terms of the integral f0 and nabla functions. its thermometric and integrating accuracy is demonstrated. a discussion of the problems associated ... | 1981 | 6111601 |
| glyceraldehyde-3-phosphate dehydrogenase. | conflicting experimental evidence of the pathway of catalysis for the enzyme from rabbit, pig and lobster muscle tissues is reviewed. transient kinetic studies with the enzyme from rabbit muscle are presented. the results are shown to be consistent with the double-displacement mechanism of catalysis originally proposed by segal & boyer (1953). the rate constant for combination of the aldehyde form of the substrate with the nad+ complex of the enzyme is about 3 x 10(7) m-1 s-1, and for all four s ... | 1981 | 6115411 |
| phosphofructokinase: structure and control. | phosphofructokinase from bacillus stearothermophilus shows cooperative kinetics with respect to the substrate fructose-6-phosphate (f6p), allosteric activation by adp, and inhibition by phosphoenolpyruvate. the crystal structure of the active conformation of the enzyme has been solved to 2.4 a resolution, and three ligand-binding sites have been located. two of these form the active site and bind the substrates f6p and atp. the third site binds both allosteric activator and inhibitor. the comple ... | 1981 | 6115424 |
| evaluation of glycine as an inactivator of glutaraldehyde. | glycine was evaluated as an inactivator of the sporicidal activity of glutaraldehyde. spores from glucose depleted cultures of bacillus stearothermophilus grown in a chemically defined medium were used. when glycine is used as an inactivator of glutaraldehyde, it lowers the ph value of the solution. glycine 1% failed to inactivate 0.5% or higher concentrations of alkaline glutaraldehyde in sporicidal studies. if viable counts cannot be performed within the first hour after inactivation, the con ... | 1982 | 6124593 |
| a comparison of heat resistance in commercially available bacillus stearothermophilus spore preparations used for monitoring steam sterilization. | | 1982 | 6124601 |
| stereochemistry of the elongation factor tu x gtp complex. | the geometry of the me2+. gtp complex at the active site of ef-tu from bacillus stearothermophilus has been investigated using thiophosphate analogs of gtp to inhibit the kirromycin-induced gtpase reaction at 60 mm nh4cl. there is no reversed selectivity for the diastereomers (rp and sp) of guanosine 5'-o-(1-thiotriphosphate) (gtp[alpha s]) on replacing mg2+ by cd2+, so that the observed specifity for the sp isomer must be due to an interaction of the pro-r oxygen of the alpha-phosphate group wi ... | 1983 | 6136409 |
| the production of alpha-amylase in batch and chemostat culture by bacillus stearothermophilus. | the production of alpha-amylase (alpha-1,4-glucan 4-glucanohydrolase; ec 3.2.1.1) by a strain of bacillus stearothermophilus isolated from leaf litter was investigated in a tryptone-maltose medium at 55 degrees c in batch and chemostat culture. amylase production was growth-limited and restricted to the exponential phase in batch culture. the enzyme yield was reduced by 40% when the culture ph was maintained at ph 7.2. amylase production in chemostat culture was influenced by the growth rate thr ... | 1980 | 6160812 |
| stringent response of bacillus stearothermophilus: evidence for the existence of two distinct guanosine 3',5'-polyphosphate synthetases. | bacillus stearothermophilus reacted to pseudomonic acid-induced inhibition of isoleucine-transfer ribonucleic acid (rna) acylation and to energy downshift caused by alpha-methylglucoside addition with accumulation of guanosine 3',5'-polyphosphates [(p)ppgpp] and restriction of rna synthesis. in vitro studies indicated that (p)ppgpp was synthesized by two different enzymes. one enzyme, (p)ppgpp synthetase i, was present in the ribosomal fraction, required the addition of a ribosome-messenger rna- ... | 1981 | 6161916 |
| the stringent response to unacylated trna, energy-and temperature-downshift in bacillus stearothermophilus. | the response of the thermophile bacillus stearothermophilus to inhibition of trna acylation, energy starvation and temperature downshift was characterized. we found that b. stearothermophilus, like other prokaryotic organisms, reacts with the so-called stringent response, which includes the accumulation of the unusual nucleotides guanosine 3',5' bis (diphosphate) [ppgpp] and guanosine 3'-diphosphate, 5'-tri-phosphate [pppgpp] and concomitantly the reduction of rna synthesis and growth rate. the ... | 1981 | 6164348 |
| reconstitution of biologically active 50s ribosomal subunits with artificial 5s rna molecules carrying disturbances in the base pairing within the molecular stalk. | bacillus stearothermophilus 50s ribosomal subunits were reconstituted in vitro using artificial 5s rna molecules constructed by combining parts of major and minor type (raué et al. (1976) europ. j. biochem. 68, 169-176) b. licheniformis 5s rna. the artificial 5s rna molecules carry defined disturbances (a.c juxtapositions and extra g.u pairs) in the base pairing between the 5'- and 3'-terminal sequences of the molecule (the molecular stalk region). the biological activity of the reconstituted su ... | 1981 | 6164987 |
| binding site of ribosomal proteins on prokaryotic 5s ribonucleic acids: a study with ribonucleases. | the binding sites of ribosomal proteins l18 and l25 on 5s rna from escherichia coli were probed with ribonucleases a, t1, and t2 and a double helix specific cobra venom endonuclease. the results for the protein-rna complexes, which were compared with those for the free rna [douthwaite, s., & garrett, r. a. (1981) biochemistry 20, 7301--7307], reveal an extensive interaction site for protein l18 and a more localized one for l25. generally comparable results, with a few important differences, were ... | 1982 | 6178424 |
| [isolation, purification and characteristics of alpha-amylase from bacillus stearothermophilus]. | | 1982 | 6189370 |
| bacillus stearothermophilus contains a plasmid-borne gene for alpha-amylase. | the gene for thermostable alpha-amylase from the thermophilic bacterium bacillus stearothermophilus has been cloned and expressed in escherichia coli. each alpha-amylase-producing colony contained at least a 9.7-kilobase-pair (kb) chimeric plasmid composed of the vector pbr322 and a common 5.4-kb hindiii fragment of dna. b. stearothermophilus contains four plasmids with sizes from 12 kb to over 108 kb. restriction endonuclease analysis of these naturally occurring plasmids showed they also conta ... | 1983 | 6193526 |
| higher order structure in the 3'-minor domain of small subunit ribosomal rnas from a gram negative bacterium, a gram positive bacterium and a eukaryote. | an experimental approach was used to determine and compare the highest order structure within the 150 to 200 nucleotides at the 3'-ends of the rnas from the small ribosomal subunits of escherichia coli, bacillus stearothermophilus and saccharomyces cerevisiae. chemical reagents were employed to establish the degree of stacking and/or accessibility of each adenosine, guanosine and cytidine. the double helices were probed with a cobra venom ribonuclease from naja naja oxiana, and the relatively un ... | 1983 | 6194304 |
| site of action of a ribosomal rna methylase responsible for resistance to erythromycin and other antibiotics. | the enzyme which confers resistance to erythromycin in the producing organism streptomyces erythraeus dimethylates a single adenine residue in bacillus stearothermophilus 23 s rrna. this corresponds to residue ade 2058 in escherichia coli 23 s rna. the methylase responsible for resistance to macrolides, lincomycin, and streptogramin b-related antibiotics in staphylococcus aureus also acts at this site. | 1983 | 6195156 |
| low temperature steam and formaldehyde sterilization. | a standard low temperature steam/formaldehyde autoclave was tested according to the manufacturer's instructions, using a range of test pieces containing bacillus stearothermophilus spores as the challenge organism. there were failures in killing the challenge organism and the reasons for these are discussed. a description of modifications made to the autoclave is given together with details of an improved operating cycle. the performance of the modified autoclave was greatly improved and conditi ... | 1983 | 6195245 |
| studies on the mode of action of a bacteriocin produced by bacillus stearothermophilus. | thermocin 10 was purified by elution from a carboxymethyl cellulose column. the eluted thermocin was homogeneous and yielded single bands with identical relative mobility when stained for protein or glycoprotein. thermocin 10 inhibited rna synthesis 3 min after its addition. cell motility, oxygen uptake, atp synthesis, and dna synthesis were inhibited 10 min after the addition of thermocin 10. total cell protein was reduced to one quarter of its normal content in cells treated with thermocin. | 1983 | 6200195 |
| reconstitution of 50 s ribosomal subunits from bacillus stearothermophilus with 5 s rna from spinach chloroplasts and low-mr rna from mitochondria of locusta migratoria and bovine liver. | reconstitution experiments with 50 s ribosomal subunits from bacillus stearothermophilus demonstrate that spinach chloroplast 5 s rrna can be incorporated into the bacterial ribosome and yield biologically active particles, thereby establishing the eubacterial nature of chloroplast 5 s rrna. in contrast, mitochondria from locusta migratoria or bovine liver do not appear to contain discrete, low-mr rnas, which can replace 5 s rrna in the functional reconstitution of b. stearothermophilus ribosome ... | 1984 | 6201395 |
| comparative structural analysis of eubacterial 5s rrna by oxidation of adenines in the n-1 position. | adenines in free 5s rrna from escherichia coli, bacillus stearothermophilus and thermus thermophilus have been oxidized at their n-1 position using monoperphthalic acid. the determination of the number of adenine 1-n-oxides was on the basis of uv spectroscopic data of the intact molecule. identification of the most readily accessible nucleotides by sequencing gel analysis reveals that they are located in conserved positions within loops, exposed hairpin loops and single-base bulge loops. implica ... | 1984 | 6201825 |
| higher-order structure in the 3'-terminal domain vi of the 23 s ribosomal rnas from escherichia coli and bacillus stearothermophilus. | an experimental approach was used to determine, and compare, the higher-order structure within domain vi of the 23 s ribosomal rnas from escherichia coli and bacillus stearothermophilus. this domain, which encompasses approximately 300 nucleotides at the 3' end of the rnas, consists of two large subdomains. the 5' subdomain has been conserved during evolution and appears to be functionally important for the binding of the ef-1 x gtp x aminoacyl-trna complex in eukaryotes. the 3' subdomain has di ... | 1984 | 6209406 |
| renaturation of the allosteric phosphofructokinase from escherichia coli. | the allosteric phosphofructokinase from escherichia coli has been renatured after complete unfolding in concentrated guanidine hydrochloride. the enzyme regains both its catalytic and regulatory abilities quantitatively. the kinetics of reactivation are biphasic and are consistent with a two-step mechanism in which a monomolecular reaction precedes a bimolecular one. the presence of atp during reactivation increases the rate at which phosphofructokinase is renatured; the second order rate consta ... | 1984 | 6232272 |
| evolution of phosphofructokinase--gene duplication and creation of new effector sites. | phosphofructokinases (pfk; ec 2.7.1.11) are tetrameric enzymes that have a key role in the regulation of glycolysis; as such, they are subject to allosteric activation and inhibition by various metabolites. eukaryotic pfks are about twice the size of prokaryotic enzymes and are regulated by a wider repertoire of effectors: for example, the subunit molecular weights of rabbit muscle (rm) pfk and bacillus stearothermophilus (bs) pfk are 82,000 and 36,000, respectively. both enzymes are activated b ... | 1984 | 6233492 |
| teicoplanin, a new antibiotic from actinoplanes teichomyceticus nov. sp. | teicoplanin, a new glycopeptide antibiotic belonging to the same family as vancomycin, inhibits cell wall synthesis in bacillus subtilis; the inhibition is accompanied by an intracellular accumulation of udp-n-acetyl-muramyl-pentapeptide. a cell-free system from bacillus stearothermophilus, capable of synthesizing peptidoglycan, is 50% inhibited by teicoplanin at 40 micrograms/ml and 100% inhibited at 100 micrograms/ml; suppression of peptidoglycan synthesis is accompanied by parallel accumulati ... | 1984 | 6240963 |
| proteolytic cleavage of methionyl transfer ribonucleic acid synthetase from bacillus stearothermophilus: effects on activity and structure. | methionyl-trna synthetase from bacillus stearothermophilus, a dimer of molecular weight 2 x 85k, is converted by limited subtilisin digestion into a fully active monomeric fragment of molecular weight 64k. the reversible methionine activation reaction of these enzymes was followed through the variation of the intensity of their trypotophan fluorescence. equilibrium and stopped-flow experiments show that the rate and mechanism for adenylate formation supported by the monomeric derivative are undi ... | 1980 | 6250575 |
| characterization of bacillus stearothermophilus plasmid pab124 and construction of deletion variants. | a restriction endonuclease cleavage map of the tetracycline resistance plasmid pab124, originally isolated from bacillus stearothermophilus, was constructed using ten enzymes. tetracycline resistance was associated with a 1 x 95 megadalton (md) region of pab124 lying between two ecori sites, and this region was circularized to produce a viable tetracycline resistance plasmid (pab224), with two ecori fragments of pab124 deleted amounting to 0 x 95 md. a second plasmid (pab524) with one ecori frag ... | 1980 | 6251158 |
| isozymes of alpha-galactosidase from bacillus stearothermophilus. | two molecular forms of alpha-galactosidase (ec 3.2.1.22) synthesized constitutively by bacillus stearothermophilus, strain at-7, have been purified. alpha-galactosidase i (with the substrate p-nitrophenyl alpha-d-galactopyranoside (pnpg)) has a ph optimum of 6 and half-life at 65 degrees c of > 2 h at low protein concentration. alpha-galactosidase ii has a ph optimum of 7 with pnpg and a half-life at 65 degrees c of about 3 min. the isozymes also differ with respect to their km with pnpg and mel ... | 1980 | 6257360 |
| the binding of nucleotides and metal ions to elongation factor tu from bacillus stearothermophilus as studied by equilibrium dialysis. | ef-tu from b. stearothermophilus binds divalent metal ions even in the absence of guanine nucleotides. the association constants necessary for characterizing the multiple equilibria between ef-tu, gdp and the divalent ions magnesium and manganese were determined by equilibrium dialysis. the constants are 4.6 x 10(4) m-1 and 5.4 x 10(5) m-1 for the binding of mg2 and 1.0 x 10(5) m-1 and 1.1 x 10(6) m-1 for the binding of mn2 to ef-tu and ef-tu . gdp, respectively. in the absence of divalent ions ... | 1981 | 6268521 |
| restriction endonuclease ecai from enterobacter cloacae. | restriction endonuclease ecai obtained from enterobacter cloacae dsm30056 recognizes the group of heptanucleotide palindromes 5'-g[unk]g-t-n-a-c-c-3', and on cleavage (arrow) produces fragments with 5'-terminal pentanucleotide extensions. it is identical in specificity with restriction endonuclease bsteii from bacillus stearothermophilus et. | 1981 | 6273787 |
| secondary structure of prokaryotic 5s ribosomal ribonucleic acids: a study with ribonucleases. | the structures of 5s ribosomal rnas from escherichia coli and bacillus stearothermophilus were examined by using ribonucleases a, t1, and t2 and a double helix specific cobra venom ribonuclease. by using both 5' and 3'-32p-end labeling methods and selecting for digested but intact 5s rna molecules, we were able to distinguish between primary and secondary cutting positions and also to establish the relative degree of cutting. the data reveal the predicted similarities of the higher order structu ... | 1981 | 6274397 |
| transformation of bacillus stearothermophilus with plasmid dna and characterization of shuttle vector plasmids between bacillus stearothermophilus and bacillus subtilis. | a thermophilic bacterium bacillus stearothermophilus ifo 12550 (atcc 12980) was transformed with each of the following plasmids, pub110 (kanamycin resistance, kmr), ptb19 (kmr and tetracycline resistance [tcr]), and its derivative ptb90 (kmr tcr), by the protoplast procedure in the presence of polyethylene glycol at 48 degrees c. the transformation frequencies per regenerant for pub110, ptb19, and ptb90 were 5.9 x 10(-3), 5.5 x 10(-3), and 2.0 x 10(-1), respectively. among these plasmids, ptb90 ... | 1982 | 6277855 |
| limited proteolysis and proton nmr spectroscopy of bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex. | the pyruvate dehydrogenase multienzyme complex of bacillus stearothermophilus was treated with chymotrypsin at ph 7 and 0 degrees c. loss of the overall catalytic activity lagged behind the rapid cleavage of the lipoate acetyltransferase polypeptide chains, whose apparent mr fell from 57 000 to 45 000 as judged by sodium dodecylsulphate/polyacrylamide gel electrophoresis. the inactive chymotrypsin-treated enzyme had lost the lipoic-acid-containing regions of the lipoate acetyltransferase chains, ... | 1982 | 6282590 |
| 6-phosphogluconate dehydrogenase from bacillus stearothermophilus. | | 1982 | 6292662 |
| acetate kinase from bacillus stearothermophilus. | | 1982 | 6296602 |
| on the dna binding protein ii from bacillus stearothermophilus. i. purification, studies in solution, and crystallization. | dna binding protein ii from bacillus stearothermophilus has been purified as a single species from the nonribosomal cell fraction by a combination of gel filtration and ion exchange chromatography. the protein occurs in solution as a tetramer and is able to bind to 30 s, 50 s, and 70 s ribosomal particles. circular dichroism studies show that the protein has approximately 45% alpha-helix. the secondary structure of the bacillus protein is considerably more resistant to the effects of increasing ... | 1983 | 6300068 |
| on the dna binding protein ii from bacillus stearothermophilus. ii. the amino acid sequence and its relation to those of homologous proteins from other prokaryotes. | the complete amino acid sequence of dna binding protein ii from bacillus stearothermophilus has been determined. the protein contains 90 amino acid residues and has a calculated mr of 9716. the sequence is compared to homologous molecules from escherichia coli, thermoplasma acidophilum, and pseudomonas aeruginosa (where only a partial sequence is available). the b. stearothermophilus molecule has 58% and 59% residues identical with the two forms of the e. coli protein and 32% with the t. acidoph ... | 1983 | 6300069 |
| molecular cloning of a thermostable neutral protease gene from bacillus stearothermophilus in a vector plasmid and its expression in bacillus stearothermophilus and bacillus subtilis. | the structural gene for a thermostable protease from bacillus stearothermophilus was cloned in plasmid ptb90. it is expressed in both b. stearothermophilus and bacillus subtilis. b. stearothermophilus carrying the recombinant plasmid produced about 15-fold more protease (310 u/mg of cell dry weight) than did the wild-type strain of b. stearothermophilus. some properties of the proteases that have been purified from the transformants of b. stearothermophilus and b. subtilis were examined. no sign ... | 1983 | 6302083 |
| the application of triazine dye affinity chromatography to the large-scale purification of glycerokinase from bacillus stearothermophilus. | gram quantities of homogeneous glycerokinase have been prepared from the thermophilic bacterium, bacillus stearothermophilus, using three major steps: precipitation of debris at ph 5.1, ion-exchange chromatography on deae-sephadex, and affinity chromatography on procion blue mx-3g-sepharose. this method is a considerable improvement over conventional techniques; the purified enzyme was obtained with a 40% recovery and a specific activity of 120 units (mumol/min)/mg protein. a modified culture me ... | 1983 | 6312842 |
| cloning and expression of a thermophilic alpha-amylase gene from bacillus stearothermophilus in escherichia coli. | a 6.4 kb hindiii fragment of bacillus stearothermophilus dy-5 dna cloned in escherichia coli using pbr322 as a vector was shown to direct the synthesis of a thermophilic alpha-amylase. in attempts to reduce the size of the insert, the alpha-amylase gene was shown to be contained in a 3.1 kb hindiii - bamhi fragment of the donor strain dna. the alpha-amylase gene was stably maintained and expressed efficiently in e. coli. the enzymic properties of alpha-amylase produced in e. coli closely resembl ... | 1984 | 6318051 |
| interaction of crystalline tyrosyl-trna synthetase with adenosine, adenosine monophosphate, adenosine triphosphate and pyrophosphate in the presence of tyrosinol. | crystalline complexes of tyrosyl-trna synthetase from bacillus stearothermophilus were prepared with adenosine, amp, atp and ppi, all in the presence of tyrosinol, which binds strongly to the tyrosine binding site but cannot be adenylated by atp. the hydrolysis of atp in the presence of crystalline tyrosyl-trna synthetase (or redissolved crystals) was checked in the absence of tyrosine or with tyrosinol. no atpase activity due to the enzyme was detected under these conditions. difference fourier ... | 1984 | 6323720 |
| q beta replicase containing a bacillus stearothermophilus elongation factor. | we purified q beta replicase containing ef-ts from bacillus stearothermophilus in place of the homologous polypeptide from escherichia coli. the hybrid enzyme was fully active in the transcription of a variety of templates. it was found to be qualitatively similar to native q beta replicase with respect to a variety of parameters which measure the efficiency of initiation of rna synthesis. the results demonstrated that q beta replicase can tolerate substantial alterations in the ef-tu x ts compo ... | 1983 | 6337116 |
| automatic diluter for bacteriological samples. | the described apparatus, carrying 190 tubes, allows automatic and aseptic dilution of liquid or suspended-solid samples. serial 10-fold dilutions are programmable from 10(-1) to 10(-9) and are carried out in glass tubes with screw caps and split silicone septa. dilution assays performed with strains of escherichia coli and bacillus stearothermophilus permitted efficient conditions for sterilization of the needle to be defined and showed that the automatic dilutions were as accurate and as reprod ... | 1983 | 6338826 |
| temperature-dependence of intramolecular coupling of active sites in pyruvate dehydrogenase multienzyme complexes. | intramolecular coupling of active sites in the pyruvate dehydrogenase multienzyme complexes of escherichia coli, ox heart and bacillus stearothermophilus was measured at various temperatures. as the temperature was raised, the extent of active-site coupling was found to increase, approaching a maximum near the physiological growth temperature of the organism. under these conditions, a single pyruvate dehydrogenase (lipoamide) dimer appeared able to cause a rapid (20s) reductive acetylation of pr ... | 1983 | 6351839 |
| structure and function of l-lactate dehydrogenases from thermophilic and mesophilic bacteria. iii) the primary structure of thermophilic lactate dehydrogenase from bacillus stearothermophilus. hydroxylamine-, o-iodosobenzoic acid- and tryptic-fragments. the complete amino-acid sequence. | based on the partial sequence of the cyanogen bromide fragments [tratschin, j.d., wirz, b., frank, g. and zuber, h. (1983) hoppe-seyler's z. physiol. chem. 364, 879-892], the amino-acid sequence of thermophilic lactate dehydrogenase from b. stearothermophilus was completed by the preparation and sequencing (sequenator, carboxypeptidase a and y) of further overlapping fragments. suitable peptide fragments were obtained by lactate dehydrogenase cleavage with hydroxylamine, o-iodosobenzoic acid and ... | 1983 | 6352452 |
| [effect of mea on the degradation of thermally-damaged dna in gamma-irradiated bacterial cells]. | it was shown that the radioprotective action of beta-mercaptoethylamine on gamma-irradiated bacillus stearothermophilus, with regard to survival and diminution of dna breaks, depended upon the temperature of incubation and the presence of thermal lesions in dna. beta-mercaptoethylamine decreased the number of both alkali-labile sites and true single-strand dna breaks in cells of bacillus stearothermophilus. beta-mercaptoethylamine decreased the number of alkali--labile sites and true single-stra ... | 1983 | 6359242 |
| steam sterilization of air turbine dental handpieces. | the efficacy of autoclaving high-speed dental handpieces was tested in two types of downward displacement steam sterilizers (instrument autoclaves), commonly used in the dentist's office. eight series of experiments were performed with various sterilization schedules. lubrication oils with or without antimicrobial agent were used in four of the series. each handpiece was inoculated with 1 ml of a suspension containing equal amounts of saliva and spores of bacillus stearothermophilus (approx. 10( ... | 1983 | 6362319 |
| molecular cloning of the glyceraldehyde-3-phosphate dehydrogenase genes of bacillus stearothermophilus and escherichia coli, and their expression in escherichia coli. | recombinant plasmids derived from pbr322, which carry gap genes coding for the d-glyceraldehyde-3-phosphate dehydrogenases (gapdh) of bacillus stearothermophilus and escherichia coli, have been isolated. the selection was carried out by complementation of an e. coli gapam mutant. two plasmids containing b. stearothermophilus and e. coli dna inserts of 4.3 kb and 1.4 kb, respectively, were characterized. transformation of the e. coli mutant with either of the recombinant plasmids lead to a very h ... | 1983 | 6363208 |
| proteins of the bacillus stearothermophilus ribosome. the amino acid sequences of proteins s5 and l30. | the amino acid sequences of ribosomal proteins s5 and l30 from bacillus stearothermophilus have been determined. these proteins have recently been crystallized in our institute. sequence data were obtained by manual sequencing of peptides derived from cyanogen bromide cleavage and digestion with trypsin and chymotrypsin or thermolysin. proteins s5 and l30 contain 166 and 62 amino acid residues and have calculated mr values of 17,628 and 7,053, respectively. comparison of the sequences with those ... | 1984 | 6363400 |
| structural homology in the amino-terminal domains of two aminoacyl-trna synthetases. | the three-dimensional structures of two animoacyl-trna synthetases, the methionyl-trna synthetase from escherichia coli (metrs) and the tyrosyl-trna synthetase from bacillus stearothermophilus (tyrrs), show a remarkable similarity over a span of about 140 amino acids. the region of homologous folding corresponds to a five-stranded parallel beta-sheet, including a mononucleotide-binding fold. one cysteine and two histidine residues that were found to be invariant in the amino acid sequences occup ... | 1983 | 6363712 |
| the complete amino acid sequences of ribosomal proteins l17, l27, and s9 from bacillus stearothermophilus. | the complete primary structures of proteins l17, l27 and s9 extracted from the bacillus stearothermophilus ribosomes with 1 m nacl and purified to homogeneity by column chromatography have been determined. the amino acid sequences of these proteins are compared to those of the homologous ribosomal proteins from escherichia coli. the number of identical amino acid residues between the homologous proteins lies between 33-55%. | 1984 | 6365549 |
| [dependency of a microbiological test of a formaldehyde gas sterilization procedure on the shape of objects to be sterilized]. | during the last decade, a number of procedures have been developed by different firms for the sterilization of heat-sensitive instruments using a mixture of formaldehyde and water vapor at a temperature of approximately 60 degrees c as means of sterilization. instruments to be sterilized by this technique as e.g. sounds and catheters normally have long narrow cavities. therefore, the formaldehyde gas sterilization procedures have to be tested primarily for their capability of achieving a suffici ... | 1983 | 6367309 |
| general stability of thermophilic enzymes: studies on 6-phosphogluconate dehydrogenase from bacillus stearothermophilus and yeast. | the thermophilic enzyme 6-phosphogluconate dehydrogenase (6-phospho-d-gluconate:nadp oxidoreductase, decarboxylating, ec 1.1.1.44) from bacillus stearothermophilus was much more resistant to inactivation under different conditions of temperature, ph, guanidine-hydrochloride, and organic solvents (dioxane, dimethylformamide, acetone) than its mesophilic counterpart from yeast. in addition, the thermophilic enzyme largely withstands proteolysis with trypsin, chymotrypsin, and elastase when compare ... | 1984 | 6386790 |
| isolation, screening and characterization of thermophilic bacillus species isolated from dairy products. | proteolytic thermophilic bacterial cultures (171 strains) were isolated from different milk and milk products. after screening these isolates for protease production in a liquid medium, fifty that exhibited enzyme activity in excess of 100 units/ml were selected and identified. twenty-nine were bacillus stearothermophilus (constituting 58% of the total), twelve were b. coagulans, five were b. circulans and four were b. licheniformis. skim milk powder contributed the maximum number of b. stearoth ... | 1984 | 6389462 |
| specific sequence homology and three-dimensional structure of an aminoacyl transfer rna synthetase. | few and limited amino acid sequence homologies have been found among eight bacterial aminoacyl transfer rna (trna) synthetases whose primary structures are known. the entire 939-amino acid primary structure of escherichia coli isoleucyl-trna synthetase is now reported. in a sequence of 11 consecutive amino acids matching a sequence in e. coli methionyl-trna synthetase, there are ten identical residues and one conservative change. this is the strongest homology recorded between any two aminoacyl ... | 1984 | 6390679 |
| application of high-performance liquid chromatographic techniques to the separation of ribosomal proteins of different organisms. | the ribosomal proteins from escherichia coli, bacillus stearothermophilus and methanococcus vannielii were separated by size-exclusion, ion-exchange and reversed-phase high-performance liquid chromatography (hplc), employing new column materials, different gradient systems, and preparative columns, respectively. the purity of the isolated proteins was analysed by one- and two-dimensional gel electrophoresis and by direct micro-sequencing. the separation of ribosomal proteins could be improved by ... | 1984 | 6397476 |
| industrial prospects for thermophiles and thermophilic enzymes. | reasons for enzyme instability are discussed. thermophiles are a promising source of more stable intracellular enzymes. this aids their purification as well as providing desirable industrial properties. the organisms themselves have advantages for high temperature fermentations, e.g. for ethanol production. systems for cloning genes into them are under development. an example is given of genetic and physiological manipulation of a fast-fermenting bacillus stearothermophilus to increase ethanol y ... | 1983 | 6400480 |
| classification of iron-sulfur cores in ferredoxins by 1h nuclear magnetic resonance spectroscopy. | a 1h nuclear magnetic resonance (nmr) study was carried out on various ferredoxins which possess one of three types of iron-sulfur clusters, (2fe-2s), (3fe-3s), or (4fe-4s). in the isolated form, (2fe-2s) ferredoxins from spinach (spinacea oleracia), pokeweed (phytolacca americana), a blue-green alga (spirulina platensis), and a halobacterium (halobacterium halobium) exhibited two broad resonances common in chemical shift at the region downfield of 10 ppm. in their reduced forms, seven contact-s ... | 1983 | 6417123 |
| the amino acid sequence of two small ribosomal proteins from bacillus stearothermophilus. | the low-mr proteins (tentatively called protein i and ii) were purified from 2 m nacl extracts of the bacillus stearothermophilus ribosome. their amino acid sequences have been determined from the peptides obtained by digestion with trypsin, chymotrypsin, and pepsin, and by cleavage with cnbr, using the micro-dabitc/pitc double-coupling method [febs lett. (1978) 93, 205-214]. protein i contains 56 residues and has an mr of 6514. protein ii had 37 residues with an mr of 4361. the amino acid seque ... | 1984 | 6420194 |
| synthesis of peptidoglycan by high molecular weight penicillin-binding proteins of bacillus subtilis and bacillus stearothermophilus. | the high molecular weight penicillin-binding proteins (pbp(s) ) bacillus subtilis pbps 1, 2, and 4 and bacillus stearothermophilus pbps 1-4 were shown to catalyze peptidoglycan synthesis from the undecaprenol-containing lipid intermediate substrate in two assay systems. in a filter paper assay system, high levels of substrate polymerization occurred when reaction mixtures were incubated on whatman 3mm filter paper. the ph optimum for peptidoglycan synthesis was 7.5 for b. subtilis pbps 1, 2, and ... | 1984 | 6420410 |
| domain structure and 1h-n.m.r. spectroscopy of the pyruvate dehydrogenase complex of bacillus stearothermophilus. | the pyruvate dehydrogenase complex of bacillus stearothermophilus was treated with staphylococcus aureus v8 proteinase, causing cleavage of the dihydrolipoamide acetyltransferase polypeptide chain (apparent mr 57 000), inhibition of the enzymic activity and disassembly of the complex. fragments of the dihydrolipoamide acetyltransferase chains with apparent mr 28 000, which contained the acetyltransferase activity, remained assembled as a particle ascribed the role of an inner core of the complex ... | 1984 | 6421282 |
| methylation of ribosomal proteins in bacteria: evidence of conserved modification of the eubacterial 50s subunit. | methylation of the 50s ribosomal proteins from bacillus stearothermophilus, bacillus subtilis, alteromonas espejiana, and halobacterium cutirubrum was measured after the cells were grown in the presence of [1-14c]methionine or [methyl-3h]methionine or both. two-dimensional polyacrylamide gel electrophoretic analysis revealed, in general, similar relative electrophoretic mobilities of the methylated proteins from each eubacterium studied. proteins known to be structurally and functionally homolog ... | 1984 | 6425271 |
| rapid assay for tetracycline in premixes and mixed feeds. | a rapid assay for tetracyclines in premixes and mixed feeds is described, which uses the extraction and dilution systems of aoac methods, and a strain of bacillus stearothermophilus atcc 12980 selected to grow at 40 degrees c. the incubation period is 4.5 h. the rapid assay yields results similar to those obtained using the aoac methods. for a 50 g chlortetracycline (ctc)/lb commercial premix, the rapid procedure averaged 109.2% of label vs 104.4% obtained using the aoac method; for a 20 g ctc/l ... | 1984 | 6430864 |
| molecular basis of isozyme formation of beta-galactosidases in bacillus stearothermophilus: isolation of two beta-galactosidase genes, bgaa and bgab. | bacillus stearothermophilus iam11001 produced three beta-galactosidases, beta-galactosidase i, ii, and iii (beta-gal i, ii, and iii), which are detectable by polyacrylamide (nondenatured) gel electrophoresis. by connecting restriction fragments of the chromosomal dna to plasmid vectors, followed by transformation of escherichia coli, two beta-galactosidase genes (bgaa and bgab) located close to each other on the chromosome were isolated. identification of the gene products and southern hybridiza ... | 1984 | 6434528 |
| examination of protein sequence homologies: i. eleven escherichia coli l7/l12-type ribosomal "a" protein sequences from eubacteria and chloroplast. | seven complete and four partial sequences of escherichia coli l7/l12-type ribosomal "a" proteins obtained from various bacteria (e. coli, bacillus subtilis, micrococcus lysodeikticus, rhodopseudomonas spheroides, desulfovibrio vulgaris, streptomyces griseus, bacillus stearothermophilus, clostridium pasteurianum, arthrobacter glacialis, and vibrio costicola) and spinach chloroplast have been reexamined using a computer program that searches for homologous tertiary structures. comparison matrices ... | 1984 | 6443314 |
| phosphofructokinase: complete amino-acid sequence of the enzyme from bacillus stearothermophilus. | the entire amino acid sequence of the protein subunit of phosphofructokinase from bacillus stearothermophilus has been established mainly by sequence analysis of cyanogen bromide fragments and of peptides derived from these fragments by further digestion with proteolytic enzymes. overlaps of the cyanogen bromide fragments as well as peptide sequences necessary to complement and to confirm tentative assignments within the larger peptide fragments were obtained from the sequences of selected pepti ... | 1980 | 6447595 |
| phosphofructokinases from lactobacteriaceae. ii. purification and properties of phosphofructokinase from streptococcus thermophilus. | phosphofructokinase (atp : d-fructose-6-phosphate 1-phosphotransferase, ec 2.7.1.11) from streptococcus thermophilus has been purified. it is a tetramer composed of identical subunits of molecular weight 36 000 and exhibits michaelis-menten kinetics. compared to the phosphofructokinases from taxonomically related bacteria, the enzyme from s. thermophilus is more stable at high temperatures. in addition, it has been demonstrated that the phosphofructokinases from lactobacteria and also from baci ... | 1981 | 6457643 |