| nutritional value of rice bran fermented by bacillus amyloliquefaciens and humic substances and its utilization as a feed ingredient for broiler chickens. | an experiment was conducted to increase the quality of rice bran by fermentation using bacillus amyloliquefaciens and humic substances and its utilization as a feed ingredient for broiler chickens. the experiment was carried out in two steps. first, the fermentation process was done using a completely randomized design in factorial with 16 treatments: i) dosage of b. amyloliquefaciens (2.10(8) cfu/g), 10 and 20 g/kg; ii) graded levels of humic substances, 0, 100, 200, and 400 ppm; iii) length of ... | 2015 | 25557819 |
| induction of salt tolerance and up-regulation of aquaporin genes in tropical corn by rhizobacterium pantoea agglomerans. | bacteria were isolated from surface disinfected seeds of eight modern corn types and an ancestor of corn, 'teosinte' and identified using 16s rdna sequences. from each of the modern corn types we obtained bacillus spp. (including, bacillus amyloliquefaciens and bacillus subtilis); while from teosinte we obtained only pantoea agglomerans and agrobacterium species. of these bacteria, only p. agglomerans could actively grow under hypersaline conditions and increase salt tolerance of tropical corn s ... | 2015 | 25557002 |
| a statistical approach for the production of thermostable and alklophilic alpha-amylase from bacillus amyloliquefaciens kcp2 under solid-state fermentation. | the bacterial strain producing thermostable, alklophilic alpha-amylase was identified as bacillus amyloliquefaciens kcp2 using 16s rdna gene sequencing data (ncbi accession no: kf112071). medium components were optimized through the statistical approach for the synthesis of alpha-amylase by the organism under solid-state fermentation using wheat bran as the substrate. the medium components influencing the enzyme production were identified using a two-level fractional factorial plackett-burman de ... | 2015 | 28324580 |
| biosurfactant production from marine bacteria associated with sponge callyspongia diffusa. | marine-derived biosurfactants have gained significant attention due to their structural and functional diversity. biosurfactant production was performed using bacteria associated with callyspongia diffusa, a marine sponge inhabiting the southern coast of india. a total of 101 sponge-associated bacteria were isolated on different media, of which 29 isolates showed positive result for biosurfactant production. among the 29 positive isolates, four were selected based on highest emusification activi ... | 2015 | 28324546 |
| an approach to low-density polyethylene biodegradation by bacillus amyloliquefaciens. | low-density polyethylene (ldpe) is a major cause of persistent and long-term environmental pollution. in this paper, two bacterial isolates bacillus amyloliquefaciens (bsm-1) and bacillus amyloliquefaciens (bsm-2) were isolated from municipal solid soil and used for polymer degradation studies. the microbial degradation ldpe was analyzed by dry weight reduction of ldpe film, change in ph of culture media, co2 estimation, scanning electron microscopy (sem), and fourier transform infrared ftir spe ... | 2015 | 28324364 |
| autochthonous gut bacteria in two indian air-breathing fish, climbing perch (anabas testudineus) and walking catfish (clarias batrachus): mode of association, identification and enzyme producing ability. | scanning electron microscopy (sem) was used to define the location of epithelium-associated bacteria in the gastrointestinal (gi) tract of two indian air-breathing fish, the climbing perch (anabas testudineus) and walking catfish (clarias batrachus). the sem examination revealed substantial numbers of rod shaped bacterial cells associated with the microvillus brush borders of enterocytes in proximal (pi) and distal regions (di) of the gi tract of both the fish species. ten (two each from the pi ... | 2015 | 26999956 |
| enhanced production of antifungal lipopeptides by bacillus amyloliquefaciens for biocontrol of postharvest disease. | food security to sustain increasing populations is a global concern. a major factor threatening food security is crop spoilage during postharvest storage. reduction of postharvest spoilage has mainly been addressed by the application of synthetic chemicals. bacillus lipopeptides, specifically lipopeptide homologues exhibiting antifungal efficacy, offer an alternative environmentally benign protocol for reduction of postharvest phytopathogens. this work is directed towards bacillus lipopeptide pr ... | 2015 | 25541516 |
| deletion of genes involved in glutamate metabolism to improve poly-gamma-glutamic acid production in b. amyloliquefaciens ll3. | here, we attempted to elevate poly-gamma-glutamic acid (γ-pga) production by modifying genes involved in glutamate metabolism in bacillus amyloliquefaciens ll3. products of rocr, rocg and gudb facilitate the conversion from glutamate to 2-oxoglutarate in bacillus subtillis. the gene odha is responsible for the synthesis of a component of the 2-oxoglutarate dehydrogenase complex that catalyzes the oxidative decarboxylation of 2-oxoglutarate to succinyl coenzyme a. in-frame deletions of these four ... | 2015 | 25540046 |
| degradation of ochratoxin a by bacillus amyloliquefaciens asag1. | ochratoxin a (ota) is widely found in food and feed products as a mycotoxin contaminant. it is produced by penicillium species and several aspergillus species. the identification ota detoxification microorganisms is believed to be the best approach for decontamination. in this study, we isolated asag1, a bacterium with the ability to degrade ota effectively, from grain depot-stored maize. a 16s rdna sequencing approach was used to identify this strain as bacillus amyloliquefaciens asag1. the deg ... | 2015 | 25517039 |
| decreased tobacco-specific nitrosamines by microbial treatment with bacillus amyloliquefaciens da9 during the air-curing process of burley tobacco. | tobacco specific nitrosamines (tsna) mainly consisting of n-nitrosonornicotine (nnn), n-nitrosoanatabine (nat), n-nitrosoanabasine (nab), and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) are a group of toxic components threatening human health. to inhibit tsna formation in tobacco leaves, a high nitrite reductive strain with low nitrate reduction ability was isolated and applied to tobacco leaves in an attempt to lower the nitrite precursor of tsna. by morphology, physiology, biochemistr ... | 2014 | 25514373 |
| detection and characterization of broad-spectrum antipathogen activity of novel rhizobacterial isolates and suppression of fusarium crown and root rot disease of tomato. | to detect and characterize broad-spectrum antipathogen activity of indigenous bacterial isolates obtained from potato soil and soya bean leaves for their potential to be developed as biofungicides to control soilborne diseases such as fusarium crown and root rot of tomato (fcrr) caused by fusarium oxysporum f. sp. radicis-lycopersici (forl). | 2015 | 25512025 |
| are cyclic lipopeptides produced by bacillus amyloliquefaciens s13-3 responsible for the plant defence response in strawberry against colletotrichum gloeosporioides? | the antagonistic strain bacillus amyloliquefaciens strain s13-3 decreased the severity of strawberry anthracnose caused by colletotrichum gloeosporioides. the foliar application of s13-3 triggered the expression of pathogenesis-related proteins, chitinase and β-1,3-glucanase, in strawberry leaves. we identified lipopeptide antibiotics, including iturin a, fengycin, mixirin, pumilacidin and surfactin, produced and secreted by s13-3. iturin a and surfactin elicited the gene expression of the patho ... | 2015 | 25511625 |
| [antagonistic acttvity of bacillus amyloliquefaciens strains--potential biocontrol agents for cereal crops]. | the antagonistic activity of strains bacillus amyloliquefaciens ykm b-7243 and ykm b-7404 against bacterial and fungal agents of winter wheat diseases was demonstrated. low antagonistic level against bacterial agents of plant diseases was shown. antifungal activity of strains against agents of winter wheat was on the low level. high biological efficiency was shown under binary compositions of the studied strains. it was concluded that the enhancing of fungicide and growth-stimulation effects in ... | 2014 | 25507808 |
| endophytic bacillus spp. produce antifungal lipopeptides and induce host defence gene expression in maize. | endophytes are mutualistic symbionts within healthy plant tissues. in this study we isolated bacillus spp. from seeds of several varieties of maize. bacillus amyloliquifaciens or bacillus subtilis were found to be present in all maize varieties examined in this study. to determine whether bacteria may produce antifungal compounds, generally lipopeptides in bacillus spp., bacterial cultures were screened for production of lipopeptides. lipopeptides were extracted by acid precipitation from liquid ... | 2015 | 25497916 |
| comparison of three bacillus amyloliquefaciens strains growth behaviour and evaluation of the spoilage risk during bread shelf-life. | this study aims at the characterisation of growth behaviour of three strains of bacillus amyloliquefaciens, isolated from ropy bread (atcc8473), wheat grain (ispa-s109.3) and semolina (ispa-n9.1) to estimate rope spoilage risk in pan bread during shelf-life using the sym'previus tool. cardinal values and growth/no growth boundaries were determined in broth, while artificial spore inoculations were performed in dough for various pan bread recipes to compare experimental counts with in silico grow ... | 2015 | 25481056 |
| identification of single nucleotide polymorphisms (snps) in the 16s rrna gene of foodborne bacillus spp. | the main goal of this work was the identification of single nucleotide polymorphisms (snps) in the 16s rrna gene of foodborne bacillus spp. that may be useful for typing purposes. these species include, among others, bacillus cereus, an important pathogenic species involved in food poisoning, and bacillus licheniformis, bacillus subtilis and bacillus pumilus, which are causative agents of food spoilage described as responsible for foodborne disease outbreaks. with this purpose in mind, 52 bacill ... | 2015 | 25475292 |
| branching patterns in leaf starches from arabidopsis mutants deficient in diverse starch synthases. | this is the first report on the cluster structure of transitory starch from arabidopsis leaves. in addition to wild type, the molecular structures of leaf starch from mutants deficient in starch synthases (ss) including single enzyme mutants ss1-, ss2-, or ss3-, and also double mutants ss1-ss2- and ss1-ss3- were characterized. the mutations resulted in increased amylose content. clusters from whole starch were isolated by partial hydrolysis using α-amylase of bacillus amyloliquefaciens. the clus ... | 2015 | 25464087 |
| immobilization of proteases on chitosan for the development of films with anti-biofilm properties. | bacterial resistance due to biofilm formation-particularly staphylococci biofilms-is associated with multiple problems in medical settings where biofilms can colonize medical indwelling devices and cause nosocomial infections. it was against this backdrop that we explored the anti-biofilm activities of a set of proteases against biofilm formation by staphylococcus aureus, listeria monocytogenes and pseudomonas aeruginosa. the selected screened enzymes were immobilized on chitosan to obtain films ... | 2015 | 25451753 |
| the dtd gene from bacillus amyloliquefaciens encodes a putative d-tyrosyl-trnatyr deacylase and is a selectable marker for bacillus subtilis. | genetically engineered microbes are of high practical importance due to their cost-effective production of valuable metabolites and enzymes, and the search for new selectable markers for genetic manipulation is of particular interest. here, we revealed that the soil bacterium bacillus amyloliquefaciens a50 is tolerant to the non-canonical amino acid d-tyrosine (d-tyr), in contrast to the closely related bacillus strain b. subtilis 168, which is a widely used "domesticated" laboratory strain. the ... | 2015 | 25441601 |
| rheological, emulsifying and thermostability properties of two exopolysaccharides produced by bacillus amyloliquefaciens lpl061. | the rheological, emulsification, thermostability and certain physicochemical properties of two purified exopolysaccharides from bacillus amyloliquefaciens lpl061 were studied. eps1 showed entangled spider mesh structure that composed of dense rope with homogeneous hexagonal particles under scanning electron microscopy. eps2 had a porous sponge structure with uniform cylindrical particles. the two exopolysaccharides showed higher intrinsic viscosity and better emulsification activity with sunflow ... | 2015 | 25439890 |
| [antagonistic acttvity of bacillus amyloliquefaciens strains--potential biocontrol agents for cereal crops]. | the antagonistic activity of strains bacillus amyloliquefaciens ykm b-7243 and ykm b-7404 against bacterial and fungal agents of winter wheat diseases was demonstrated. low antagonistic level against bacterial agents of plant diseases was shown. antifungal activity of strains against agents of winter wheat was on the low level. high biological efficiency was shown under binary compositions of the studied strains. it was concluded that the enhancing of fungicide and growth-stimulation effects in ... | 2014 | 25434210 |
| role of two amino acid residues' insertion on thermal stability of thermophilic α-amylase amy121 from a deep sea bacterium bacillus sp. scsio 15121. | α-amylases from bacillus licheniformis (bla) and bacillus amyloliquefaciens (baa) are both important industrial enzymes with high similarity in structure but significant differences in thermostability. the mechanisms underlying this discrepancy are still poorly understood. here, we investigated the role of two amino acids' insertion on the thermostability of these two group amylases. a newly obtained thermophilic amylase amy121 was found much closer to bla in both primary structure and enzymolog ... | 2015 | 25421163 |
| application of statistically based experimental designs to optimize cellulase production and identification of gene. | a natural bacterial strain identified as bacillus amyloliquefaciens mbaa3 using 16s rdna partial genome sequencing has been studied for optimization of cellulase production. statistical screening of media components for production of cellulase by b. amyloliquefaciens mbaa3 was carried out by plackett-burman design. plackett-burman design showed cmc, mgso4 and ph as significant components influencing the cellulase production from the media components screened by plackett-burman fractional factori ... | 2014 | 25416137 |
| biosurfactins production by bacillus amyloliquefaciens r3 and their antibacterial activity against multi-drug resistant pathogenic e. coli. | in this work, the anti-escherichia coli activity of the bioactive substances produced by bacillus amyloliquefaciens r3 was examined. a new and cheap medium for production of the anti-e. coli substances which contained 20.0 g l(-1) soybean powder, 20.0 g l(-1) wheat flour, ph 6.0 was developed. a crude surfactant concentration of 0.48 mg ml(-1) was obtained after 27 h of 10-l fermentation, and the diameter of the clear zone on the plate seeded with the pathogenic e. coli 2# was 23.3 mm. a prelimi ... | 2015 | 25407729 |
| differential proteomics analysis of bacillus amyloliquefaciens and its genome-shuffled mutant for improving surfactin production. | genome shuffling technology was used as a novel whole-genome engineering approach to rapidly improve the antimicrobial lipopeptide yield of bacillus amyloliquefaciens. comparative proteomic analysis of the parental es-2-4 and genome-shuffled fmb38 strains was conducted to examine the differentially expressed proteins. the proteome was separated by 2-de (two dimensional electrophoresis) and analyzed by ms (mass spectrum). in the shuffled strain fmb38, 51 differentially expressed protein spots wit ... | 2014 | 25365175 |
| two novel exopolysaccharides from bacillus amyloliquefaciens c-1: antioxidation and effect on oxidative stress. | two odorless, water-soluble exopolysaccharide (eps) fractions, eps-1 and eps-2, were isolated from a newly isolated bacillus amyloliquefaciens strain c-1 and purified by ion exchange and gel chromatography. the purified eps-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and possessed a molecular weight of 79.6 kda, while eps-2 contained only glucose and mannose in a 3:1 ratio, with the molecular weights of 19.8 kda. the antioxidant activity results showed t ... | 2015 | 25362510 |
| genome sequence of bacillus amyloliquefaciens gb03, an active ingredient of the first commercial biological control product. | bacillus amyloliquefaciens gb03 has been used as a representative commercialized strain of the bacilli for biological control against a broad spectrum of plant pathogens and as a bio-fertilizer to promote growth and yield of field crops for more than two decades. herein, we present the genome sequence and a brief analysis of strain gb03. | 2014 | 25359911 |
| bombyx mori nucleopolyhedrovirus bacmid enabling rapid generation of recombinant virus by in vitro transposition. | a novel recombinant bacmid, beasybm, that enables the easy and fast generation of pure recombinant baculovirus without any purification step was constructed. in beasybm, attr recombination sites were introduced to facilitate the generation of a recombinant viral genome by in vitro transposition. moreover, the extracellular rnase gene from bacillus amyloliquefaciens, barnase, was expressed under the control of the cotesia plutellae bracovirus early promoter to negatively select against the nonrec ... | 2015 | 25341472 |
| draft genome sequence of the ionic liquid-tolerant bacterium bacillus amyloliquefaciens cmw1. | here, we report the draft genome sequence of an ionic liquid-tolerant bacterium, bacillus amyloliquefaciens cmw1, which is newly isolated from a japanese fermented soybean paste. the genome sequence will allow for a characterization of the molecular mechanism of its ionic liquid tolerance. | 2014 | 25323721 |
| suppression of bacterial wilt of tomato by bioorganic fertilizer made from the antibacterial compound producing strain bacillus amyloliquefaciens hr62. | ralstonia solanacearum (smith) is an important soil-borne pathogen worldwide. we investigated the effects of a new bioorganic fertilizer, bio62, which was made from organic fertilizer and antagonist bacillus amyloliquefaciens hr62, on the control of bacterial wilt of tomato in greenhouse condition. the results showed that the application of bio62 significantly decreased disease incidence by 65% and strongly reduced r. solanacearum populations both in the rhizosphere soil (8.04 log cfu g(-1) dry ... | 2014 | 25322261 |
| comparative proteomics analysis of bacillus amyloliquefaciens sqr9 revealed the key proteins involved in in situ root colonization. | bacillus amyloliquefaciens sqr9 is a well-investigated plant growth-promoting rhizobacteria with strong root colonization capability. to identify the key proteins involved in in situ root colonization and biofilm formation, the proteomic profiles of planktonic and root colonized sqr9 cells were compared. a total of 755 proteins were identified, of which 78 and 95 proteins were significantly increased and deceased, respectively, when sqr9 was colonized on the root. the proteins that were closely ... | 2014 | 25299960 |
| studies of plant colonisation by closely related bacillus amyloliquefaciens biocontrol agents using strain specific quantitative pcr assays. | certain strains of bacillus amyloliquefaciens can colonize plants and improve growth and stress management. in order to study these effects, bacterial growth dynamics on plants and in the rhizosphere are of interest calling for specific analytical tools. for that purpose, quantitative real-time pcr (qpcr) assays were developed in order to differentiate among three closely related b. amyloliquefaciens subsp. plantarum strains (ucmb5033, ucmb5036, ucmb5113) and to determine their levels with high ... | 2014 | 25294724 |
| induced systemic resistance responses in perennial ryegrass against magnaporthe oryzae elicited by semi-purified surfactin lipopeptides and live cells of bacillus amyloliquefaciens. | the suppressive ability of several strains of cyclic lipopeptide-producing bacillus rhizobacteria to grey leaf spot disease caused by magnaporthe oryzae has been documented previously; however, the underlying mechanism(s) involved in the induced systemic resistance (isr) activity in perennial ryegrass (lolium perenne l.) remains unknown. root-drench application of solid-phase extraction (spe)-enriched surfactin and live cells of mutant bacillus amyloliquefaciens strain fzb42-ak3 (produces surfac ... | 2015 | 25285593 |
| [antagonism against beauveria bassiana by lipopeptide metabolites produced by entophyte bacillus amyloliquefaciens strain swb16]. | we screened bacterial strains that have strong antagonism against beauveria bassiana, an important pathogen of silkworm industry, and detected the antagonistic activity of lipopeptide metabolites. | 2014 | 25252459 |
| binase-like guanyl-preferring ribonucleases are new members of bacillus phop regulon. | extracellular low-molecular weight guanyl-preferring ribonucleases (lmw rnases) of bacillus sp. comprise a group of hydrolytic enzymes that share highly similar structural and catalytic characteristics with barnase, a ribonuclease from bacillus amyloliquefaciens, and binase, a ribonuclease from bacillus intermedius. although the physical-chemical and catalytic properties of bacillus guanyl-preferring ribonucleases are very similar, there is considerably more variation in the environmental condit ... | 2015 | 25238955 |
| comparative proteomic analysis of antagonistic bacillus amyloliquefaciens q-426 cultivated under different ph conditions. | bacillus amyloliquefaciens q-426 produces lipopeptide compounds with antifungal activities. initial ph value has a significant influence on the production of lipopeptide compounds. the correlation between ph and intrinsic mechanism of lipopeptide production was rarely discussed. in this research, comparative proteomics, using two-dimensional gel electrophoresis and mass spectrometry, was applied to identify b. amyloliquefaciens q-426 intracellular proteins differentially expressed under initial ... | 2015 | 25211522 |
| pyrosequencing analysis of microbiota reveals that lactic acid bacteria are dominant in korean flat fish fermented food, gajami-sikhae. | the gajami-sikhae, traditional korean cuisine fermented with flat fish, samples were collected from eight different manufacturers (gs1-gs8). we employed pyrosequencing method to analyze the bacterial communities of the gajami-sikhae samples. family- and genus-level analyses indicated that the bacterial community compositions of gs3 and gs6 were distinct from those of the rest. the species-level structures of bacterial communities of the gajami-sikhae samples except for gs3 and gs6 featured lacto ... | 2014 | 25209511 |
| functional expression of a novel alkaline-adapted lipase of bacillus amyloliquefaciens from stinky tofu brine and development of immobilized enzyme for biodiesel production. | using enrichment procedures, a lipolytic strain was isolated from a stinky tofu brine and was identified as bacillus amyloliquefaciens (named b. amyloliquefaciens nsic-8) by morphological, physiological, biochemical tests and 16s rdna sequence analysis. meanwhile, the key enzyme gene (named lip ba) involved in ester metabolism was obtained from nsic-8 with the assistance of homology analysis. the novel gene has an open reading frame of 645 bp, and encodes a 214-amino-acid lipase (lipba). the ded ... | 2014 | 25199563 |
| metabolic characterization of bacillus subtilis and bacillus amyloliquefaciens strains isolated from traditional dry-cured sausages. | the aim of this study was to investigate the effect of ph, temperature, and nacl on growth, proteolytic and lipolytic activities, and the ability to produce biogenic amines of 19 strains of bacillus isolated from androlla and botillo (two spanish traditional sausages) to elucidate the role of these bacteria in sausage manufacture. all strains grew in the presence of 10% salt and at ph values of 5.0 and 5.5, whereas only 9 strains grew at 10°c. proteolytic activity was assessed by the agar plate ... | 2014 | 25198855 |
| draft genome sequence of bacillus amyloliquefaciens hb-26. | bacillus amyloliquefaciens hb-26, a gram-positive bacterium was isolated from soil in china. sds-page analysis showed this strain secreted six major protein bands of 65, 60, 55, 34, 25 and 20 kda. a bioassay of this strain reveals that it shows specific activity against p. brassicae and nematode. here we describe the features of this organism, together with the draft genome sequence and annotation. the 3,989,358 bp long genome (39 contigs) contains 4,001 protein-coding genes and 80 rna genes. | 2014 | 25197462 |
| field evaluation of the bacterial volatile derivative 3-pentanol in priming for induced resistance in pepper. | plants are defended from attack by emission of volatile organic compounds (vocs) that can act directly against pathogens and herbivores or indirectly by recruiting natural enemies of herbivores. however, microbial voc have been less investigated as potential triggers of plant systemic defense responses against pathogens in the field. bacillus amyloliquefaciens strain in937a, a plant growth-promoting rhizobacterium that colonizes plant tissues, stimulates induced systemic resistance (isr) via its ... | 2014 | 25149655 |
| double substituted variant of bacillus amyloliquefaciens esterase with enhanced enantioselectivity and high activity towards 1-(3',4'-methylenedioxyphenyl)ethyl acetate. | bacillus amyloliquefaciens esterase (bae) was applied to produce (r)-1-(3',4'-methylenedioxyphenyl)ethanol, a chiral drug intermediate. in this study, we improved the enantioselectivity of bae by protein engineering instead of process engineering as used in our previous work. saturation mutagenesis was carried out on eight positions of bae based on structure modeling and substrate docking. a double substituted variant v10 (k358d/a396c) showed an excellent enantioselectivity without decreasing th ... | 2015 | 25104035 |
| antiherpes simplex virus type 2 activity of the antimicrobial peptide subtilosin. | in this study, we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from bacillus amyloliquefaciens, against herpes simplex virus type 2 (hsv-2) in cell cultures and we investigated subtilosin mode of action. | 2014 | 25087911 |
| isolation of antifungal bacteria from japanese fermented soybeans, natto. | an inhibitory effect of a traditional japanese fermented food, natto, was found against plant pathogens such as rhizoctonia solani and fusarium oxysporum, and the bacteria which showed inhibition were isolated from the natto. among isolated bacteria, bc-1 and gac exhibited a strong antagonistic effect in vitro against plant pathogens on an agar medium. the supernatant of bacterial culture also showed strong activity against r. solani, which meant the antimicrobial substances were produced and se ... | 2013 | 25078814 |
| isolation and characterization of putative endophytic bacteria antagonistic to phoma tracheiphila and verticillium albo-atrum. | a collection of 200 bacterial isolates recovered from citrus plants (citrus limon, citrus sinensis, and citrus reticulata), medicago truncatula and laurus nobilis, was established. in vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens phoma tracheiphila and verticillium albo-atrum. isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (pgpb) abilities, as well as for the presence of nonribosomal pe ... | 2014 | 25074353 |
| occurrence of bacillus amyloliquefaciens as a systemic endophyte of vanilla orchids. | we report the occurrence of bacillus amyloliquefaciens in vanilla orchids (vanilla phaeantha) and cultivated hybrid vanilla (v. planifolia × v. pompona) as a systemic bacterial endophyte. we determined with light microscopy and isolations that tissues of v. phaeantha and the cultivated hybrid were infected by a bacterial endophyte and that shoot meristems and stomatal areas of stems and leaves were densely colonized. we identified the endophyte as b. amyloliquefaciens using dna sequence data. si ... | 2014 | 25060609 |
| draft genome sequence of bacillus amyloliquefaciens ebl11, a new strain of plant growth-promoting bacterium isolated from rice rhizosphere. | bacillus amyloliquefaciens strain ebl11 is a bacterium that can promote plant growth by inhibiting the growth of fungi on plant surfaces and providing nutrients as a nonchemical biofertilizer. the estimated genome of this strain is 4.05 mb in size and harbors 3,683 coding genes (cdss). | 2014 | 25059875 |
| simultaneous enhanced catalytic activity and thermostability of a 1,3-1,4-β-glucanase from bacillus amyloliqueformis by chemical modification of lysine residues. | the thermostablility and enzymatic activity of 1,3-1,4-β-glucanase (bgla) from bacillus amyloliquefaciens was improved by modifying five (out of 12) ε-amino groups in lysine residues with nitrous acid. the optimal modification condition for bgla was determined as 30 mm nitrous acid at, 40 °c for 30 min. the optimally-modified bgla had higher specific activity and t 50 value, which were 3,370 u/mg and 70 °c, respectively. its half-life values at 50 and 60 °c were extended and reached 58.5 and 49. ... | 2014 | 25048240 |
| construction of a novel secretion expression system guided by native signal peptide of phod in zymomonas mobilis. | in the current study, three native signal peptides (sps) from phoc, phod, and zmo0331were investigated and compared to construct novel secretion expression systems in zymomonas mobilis. the secretion expression of target protein, α-amylase from bacillus amyloliquefaciens (baa), guided by phod's sp resulted in more hydrolysis of starch than that by the other two sps. extracellular and intracellular α-amylase activities of the strain containing phod's sp were also higher than the other two strains ... | 2014 | 25036971 |
| metabolic engineering of bacillus amyloliquefaciens for poly-gamma-glutamic acid (γ-pga) overproduction. | we constructed a metabolically engineered glutamate-independent bacillus amyloliquefaciens strain with considerable γ-pga production. it was carried out by double-deletion of the cwlo gene and epsa-o cluster, as well as insertion of the vgb gene in the bacteria chromosome. the final generated strain nk-pv elicited the highest production of γ-pga (5.12 g l(-1)), which was 63.2% higher than that of the wild-type nk-1 strain (3.14 g l(-1)). the γ-pga purity also improved in the nk-pv strain of 80.4 ... | 2014 | 24986065 |
| draft genome sequence of bacillus amyloliquefaciens b-1895. | in this report, we present a draft genome sequence of bacillus amyloliquefaciens strain b-1895. comparison with the genome of a reference strain demonstrated similar overall organization, as well as differences involving large gene clusters. | 2014 | 24948774 |
| the bacterial lipopeptide iturins induce verticillium dahliae cell death by affecting fungal signalling pathways and mediate plant defence responses involved in pathogen-associated molecular pattern-triggered immunity. | verticillium wilt in cotton caused by verticillium dahliae is one of the most serious plant diseases worldwide. because no known fungicides or cotton cultivars provide sufficient protection against this pathogen, v. dahliae causes major crop yield losses. here, an isolated cotton endophytic bacterium, designated bacillus amyloliquefaciens 41b-1, exhibited greater than 50% biocontrol efficacy against v. dahliae in cotton plants under greenhouse conditions. through high-performance liquid chromato ... | 2015 | 24934960 |
| effects of bacillus amyloliquefaciens as a probiotic strain on growth performance, cecal microflora, and fecal noxious gas emissions of broiler chickens. | this experiment was conducted to investigate the effects of bacillus amyloliquefaciens probiotic (bap) as a direct-fed microbial on growth performance, cecal microflora, serum immunoglobulin levels, and fecal noxious gas emissions of broiler chickens. a total of 400 one-day-old broiler chicks (ross 308) were randomly assigned to 1 of 5 treatment diets formulated to supply 0, 1, 5, 10, and 20 g/kg of bap and were fed for 35 d. each treatment had 8 replicate pens with 10 birds per replicate. on co ... | 2014 | 24902704 |
| impact of bacillus amyloliquefaciens s13-3 on control of bacterial wilt and powdery mildew in tomato. | biological control is a non-hazardous technique to control plant diseases. researchers have explored microorganisms that show high plant-disease control efficiency for use as biological control agents. | 2015 | 24889125 |
| biocontrol traits and antagonistic potential of bacillus amyloliquefaciens strain njzjsb3 against sclerotinia sclerotiorum, a causal agent of canola stem rot. | bacillus amyloliquefaciens strain njzjsb3 has shown antagonism of several phytopathogens in vitro, especially sclerotinia sclerotiorum. both the broth culture and cell suspension of strain njzjsb3 could completely protect the detached leaves of canola (brassica napus) from s. sclerotiorum infection. in pot experiments, the application of strain njzjsb3 cell suspension (10(8) cfu/ml) decreased the disease incidence by 83.3%, a result similar to commercially available fungicide (dimetachlone). in ... | 2014 | 24861342 |
| a markerless gene replacement method for b. amyloliquefaciens ll3 and its use in genome reduction and improvement of poly-γ-glutamic acid production. | we herein adapted a markerless gene replacement method by combining a temperature-sensitive plasmid pksv7 with a counterselectable marker, the upp gene encoding uracil phosphoribosyltransferase (uprtase), for the poly-γ-glutamic acid (γ-pga)-producing strain bacillus amyloliquefaciens ll3. deletion of the upp gene conferred ll3 5-fluorouracil (5-fu) resistance. sensitivity to 5-fu was restored when ll3 δupp was transformed with pksv7-based deletion plasmid which carries a functional allele of th ... | 2014 | 24859524 |
| substitutional analysis of the c-terminal domain of abrb revealed its essential role in dna-binding activity. | the global transition state regulator abrb controls more than 100 genes of the bacillus relatives and is known to interact with varying dna-sequences. the dna-binding domain of the abrb-like proteins was proposed to be located exclusively within the amino-terminal ends. however, the recognition of dna, and specificity of the binding mechanism, remains elusive still in view of highly differing recognition sites. here we present a substitutional analysis to examine the role of the carboxy-terminal ... | 2014 | 24832089 |
| starter culture selection for making chinese sesame-flavored liquor based on microbial metabolic activity in mixed-culture fermentation. | selection of a starter culture with excellent viability and metabolic activity is important for inoculated fermentation of traditional food. to obtain a suitable starter culture for making chinese sesame-flavored liquor, the yeast and bacterium community structures were investigated during spontaneous and solid-state fermentations of this type of liquor. five dominant species in spontaneous fermentation were identified: saccharomyces cerevisiae, pichia membranaefaciens, issatchenkia orientalis, ... | 2014 | 24814798 |
| functions of poly-gamma-glutamic acid (γ-pga) degradation genes in γ-pga synthesis and cell morphology maintenance. | poly-γ-glutamic acid (γ-pga) is an important biopolymer with greatly potential in industrial and medical applications. in the present study, we constructed a metabolically engineered glutamate-independent bacillus amyloliquefaciens ll3 strain with considerable γ-pga production, which was carried out by single, double, and triple markerless deletions of three degradation genes pgds, ggt, and cwlo. the highest γ-pga production (7.12 g/l) was obtained from the pgds and cwlo double-deletion strain n ... | 2014 | 24769902 |
| spatiotemporal monitoring of the antibiome secreted by bacillus biofilms on plant roots using maldi mass spectrometry imaging. | some soil bacilli living in association with plant roots can protect their host from infection by pathogenic microbes and are therefore being developed as biological agents to control plant diseases. the plant-protective activity of these bacteria has been correlated with the potential to secrete a wide array of antibiotic compounds upon growth as planktonic cells in isolated cultures under laboratory conditions. however, in situ expression of these antibiotics in the rhizosphere where bacterial ... | 2014 | 24712753 |
| recovery of several cell pellet-associated antibiotics produced by bacillus amyloliquefaciens njn-6. | in this study, six antibiotics which are antagonistic to phytopathogens were extracted from the water-washed cell pellets of bacillus amyloliquefaciens njn-6. they were purified by hplc and identified using electrospray ionization mass spectroscopy (esi-ms). besides three iturin a homologous and macrolactin a, two other kinds of antibiotics were extracted from the cell pellets of b. amyloliquefaciens njn-6. an antifungal compound with the molecular mass of 1072 da was identified as bacillomycin ... | 2014 | 24702101 |
| inhibitory efficacy of cyclo(l-leucyl-l-prolyl) from mangrove rhizosphere bacterium-bacillus amyloliquefaciens (mms-50) toward cariogenic properties of streptococcus mutans. | since streptococcus mutans is the principal etiologic agent causing dental caries, by encompassing an array of unique virulence traits, emerging treatment strategies that specifically target the virulence of this pathogen may be promising as alternative approaches compared to conventional antibiotic therapy. in this perspective, we investigated chloroform extract of cell-free culture supernatant from mangrove rhizosphere bacterium bacillus amyloliquefaciens (mms-50) in terms of anticariogenic pr ... | 2014 | 24698790 |
| two new cyclic tetrapeptides from deep-sea bacterium bacillus amyloliquefaciens gas 00152. | | 2014 | 24690914 |
| characteristics and antioxidant effect of garlic in the fermentation of cheonggukjang by bacillus amyloliquefaciens mj1-4. | the changes in the β-glucosidase activity, total phenolic contents, isoflavone contents, and antioxidant activities during the fermentation of cheonggukjang by bacillus amyloliquefaciens mj1-4 with and without garlic were investigated. the levels of total phenolic and isoflavonemalonylglycoside, -acetylglycoside, and -aglycone contents increased, whereas the 2,2-diphenyl- 1-picrylhydrazyl (dpph) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (abts) radical scavenging activities and f ... | 2014 | 24690635 |
| distribution of branches in whole starches from maize mutants deficient in starch synthase iii. | an earlier study explored the possibility of analyzing the distribution of branches directly in native, whole starch without isolating the amylopectin component. the aim of this study was to explore if this approach can be extended to include starch mutants. whole starches from du1 maize mutants deficient in starch synthase iii (ssiii) with amylose content of ∼30-40% were characterized and compared with the wild type of the common genetic background w64a. clusters were produced from whole starch ... | 2014 | 24684540 |
| complete genome sequence for the fusarium head blight antagonist bacillus amyloliquefaciens strain trigocor 1448. | we present the complete genome sequence for bacillus amyloliquefaciens trigocor 1448 (atcc 202152), a bacterial biological control agent for fusarium head blight in wheat. we compare it to its closest relative, b. amyloliquefaciens strain as43.3. | 2014 | 24675861 |
| [effects of ph and oxygen supply on production of 2,3-butanediol from biodiesel-derived glycerol by bacillus amyloliquefaciens]. | bacillus amyloliquefaciens b10-127 was used to produce 2,3-butanediol (2,3-bd) from residual glycerol obtained from biodiesel synthesis. important variables for 2,3-bd fermentation, ph and dissolved oxygen, were studied. when ph was maintained constant, the yield of 2,3-bd was inhibited. the highest 2,3-bd yields were achieved by fermentation without any ph control with an optimized initial ph 6.5. batch fermentative production of 2,3-bd by b. amyloliquefaciens was investigated using various oxy ... | 2013 | 24660634 |
| nutrient-induced spore germination of a bacillus amyloliquefaciens biocontrol agent on wheat spikes. | in this study, we investigated the feasibility of applying nutrient germinants to plant surfaces to stimulate the spore germination of the plant disease biocontrol agent bacillus amyloliquefaciens strain trigocor. | 2014 | 24641281 |
| isolation of bacillus amyloliquefaciens s20 and its application in control of eggplant bacterial wilt. | bacterial strain s20 was isolated and identified as bacillus amyloliquefaciens based on physiological and biochemical characteristics and a 16s rrna gene sequence analysis. strain s20 inhibits the growth of fusarium oxysporum and ralstonia solanacearum. some genes associated with the synthesis of some lipopeptides were detected in strain s20 by pcr. iturins a were identified as the main antagonistic substrates by analysis with electrospray ionization mass spectrometry/collision-induced dissociat ... | 2014 | 24632400 |
| lipopeptides from bacillus strain ar2 inhibits biofilm formation by candida albicans. | the ability of the human fungal pathogen candida albicans to reversibly switch between different morphological forms and establish biofilms is crucial for establishing infection. targeting phenotypic plasticity and biofilm formation in c. albicans represents a new concept for antifungal drug discovery. the present study evaluated the influence of cyclic lipopeptide biosurfactant produced by bacillus amyloliquefaciens strain ar2 on c. albicans biofilms. the biosurfactant was characterized as a mi ... | 2014 | 24623107 |
| rapid total synthesis of darpin pe59 and barnase. | we report the convergent total synthesis of two proteins: darpin pe59 and bacillus amyloliquefaciens rnase (barnase). leveraging our recently developed fast-flow peptide-synthesis platform, we rapidly explored numerous conditions for the assembly of long polypeptides, and were able to mitigate common side reactions, including deletion and aspartimide products. we report general strategies for improving the synthetic quality of difficult peptide sequences with our system. high-quality protein fra ... | 2014 | 24616257 |
| rapid flow-based peptide synthesis. | a flow-based solid-phase peptide synthesis methodology that enables the incorporation of an amino acid residue every 1.8 min under automatic control or every 3 min under manual control is described. this is accomplished by passing a stream of reagent through a heat exchanger into a low volume, low backpressure reaction vessel, and through a uv detector. these features enable continuous delivery of heated solvents and reagents to the solid support at high flow rate, thereby maintaining maximal co ... | 2014 | 24616230 |
| expression of a pathogen-induced cysteine protease (adcp) in tapetum results in male sterility in transgenic tobacco. | usable male sterility systems have immense potential in developing hybrid varieties in crop plants, which can also be used as a biological safety containment to prevent horizontal transgene flow. barnase-barstar system developed earlier was the first approach to engineer male sterility in plants. in an analogous situation, we have evolved a system of inducing pollen abortion and male sterility in transgenic tobacco by expressing a plant gene coding for a protein with known developmental function ... | 2014 | 24615687 |
| enhanced rhizosphere colonization of beneficial bacillus amyloliquefaciens sqr9 by pathogen infection. | root exudates play important roles in root-soil microorganism interactions and can mediate tripartite interactions of beneficial microorganisms-plant-pathogen in the rhizosphere. however, the roles of organic acid components in this process have not been well studied. in this study the colonization of a plant growth-promoting rhizobacterium, bacillus amyloliquefaciens sqr9, on cucumber root infected by fusarium oxysporum f. sp. cucumerinum j. h. owen (foc) was investigated. chemotaxis and biofil ... | 2014 | 24612247 |
| purification and partial characterization of a chymotrypsin-like serine fibrinolytic enzyme from bacillus amyloliquefaciens fcf-11 using corn husk as a novel substrate. | a non-toxic, direct-acting fibrinolytic enzyme, fcf-11, from a newly isolated bacillus amyloliquefaciens fcf-11 was purified, characterized and assayed both in vitro and in vivo for its thrombolytic potential. corn husk was used as for the first time as the sole carbon/nitrogen source for enzyme production. the molecular weight of the purified enzyme was 18.2 kda and purification increased its specific activity 443.5-fold with a recovery of 17 %. maximal activity was attained at a temperature of ... | 2014 | 24609498 |
| construction of individual, fused, and co-expressed proteins of endoglucanase and β-glucosidase for hydrolyzing sugarcane bagasse. | at least a combination of endoglucanase (eglii) and β-glucosidase (bglz) is required for hydrolyzing crystalline cellulose. to understand the catalytic efficiency of combination enzymes for converting biomass to sugars, eglii and bglz were constructed in the form of individual, fused as well as co-expression proteins, and their activities for hydrolyzing sugarcane bagasse were evaluated. the genes, eglii isolated from bacillus amyloliquefaciens psm3.1 earlier and bglz from b. amyloliquefaciens a ... | 2016 | 24598011 |
| optimization of medium components for production of chitin deacetylase by bacillus amyloliquefaciens z7, using response surface methodology. | plackett-burman design and box-behnken response surface methodology (rsm) was employed to optimize the medium components for the chitin deacetylase (cda) activity from bacillus amyloliquefaciens z7. plackett-burman design was applied to determine the specific medium components affecting cda activity and found that starch, chitin and mgso4 were critical in augmenting cda activity. these significant parameters were further optimized using box-behnken rsm and the optimum concentrations of starch, c ... | 2014 | 26740755 |
| isolation of anteiso-c17, iso-c17, iso-c16, and iso-c15 bacillomycin d from bacillus amyloliquefaciens sd-32 and their antifungal activities against plant pathogens. | two new cyclic lipopeptides (3 and 4) were isolated from the culture filtrate of bacillus amyloliquefaciens strain sd-32, together with two known metabolites, iso-c15 and iso-c16 bacillomycin d (1 and 2). spectroscopic and chemical analyses identified the structures of the new compounds 3 and 4 as anteiso-c17 bacillomycin d, cyclic (l-asn-d-tyr-d-asn-l-pro-l-glu-d-ser-l-thr-3-amino-14-methylhexadecanoic acid) and iso-c17 bacillomycin d, cyclic (l-asn-d-tyr-d-asn-l-pro-l-glu-d-ser-l-thr-3-amino-1 ... | 2014 | 24548079 |
| high-pressure thermal sterilization: food safety and food quality of baby food puree. | the benefits that high-pressure thermal sterilization offers as an emerging technology could be used to produce a better overall food quality. due to shorter dwell times and lower thermal load applied to the product in comparison to the thermal retorting, lower numbers and quantities of unwanted food processing contaminants (fpcs), for example, furan, acrylamide, hmf, and mcpd-esters could be formed. two spore strains were used to test the technique; geobacillus stearothermophilus and bacillus a ... | 2014 | 24547697 |
| synthesis of biocontrol macromolecules by derivative of chitosan with surfactin and antifungal evaluation. | a derivative of chitosan was prepared with chitosan and β-cyclodextrins, which was synthesized by the immobilization reaction, as a carrier to adsorb surfactin produced from bacillus amyloliquefaciens and got biological macromolecules. the antifungal activity against three sapstain fungi by a combination of macromolecules was tested. the results showed that the macromolecules inhibited the mycelium growth of sapstain fungi lasiodiplodia rubropurpurea, l. crassispora, and l. theobromae by about 7 ... | 2014 | 24530369 |
| whole-genome shotgun sequence of bacillus amyloliquefaciens strain uasws ba1, a bacterium antagonistic to plant pathogenic fungi. | we report here the whole-genome shotgun sequence of bacillus amyloliquefaciens strain uasws ba1, isolated from inner wood tissues of a decaying platanus × acerifolia tree. this strain proved to be antagonistic to several plant pathogenic fungi and oomycetes and can be developed as a biological control agent in agriculture. | 2014 | 24503983 |
| influence of ph and temperature on the activity of sno2-bound α-amylase: a genotoxicity assessment of sno2 nanoparticles. | immobilization of biologically important molecules on a myriad of nanosized materials has attracted great attention due to their small size, biocompatibility, higher surface-to-volume ratio, and lower toxicity. these properties make nanoparticles (nps) a superior matrix over bulk material for the immobilization of enzymes and proteins. in the present study, bacillus amyloliquefaciens α-amylase was immobilized on sno2 nanoparticles by a simple adsorption mechanism. nanoparticle-adsorbed enzyme re ... | 2014 | 24499361 |
| biocontrol activity of bacillus amyloliquefaciens cnu114001 against fungal plant diseases. | a total of 62 bacterial isolates were obtained from gomsohang mud flat, mohang mud flat, and jeju island, republic of korea. among them, the isolate cnu114001 showed significant antagonistic activity against pathogenic fungi by dual culture method. the isolate cnu114001 was identified as bacillus amyloliquefaciens by morphological observation and molecular data analysis, including 16srdna and gyrasea (gyra) gene sequences. antifungal substances of the isolate were extracted and purified by silic ... | 2013 | 24493945 |
| complete genome sequence of bacillus amyloliquefaciens lfb112 isolated from chinese herbs, a strain of a broad inhibitory spectrum against domestic animal pathogens. | bacillus amyloliquefaciens lfb112, isolated from chinese herbs, displays a broad inhibitory activity against an array of pathogens involved in domestic animal diseases. here, we present the complete genome sequence of b. amyloliquefaciens lfb112, providing insights into the genomic basis of its effects and facilitating its application in animal production. | 2014 | 24463274 |
| characteristics of miniature cheddar-type cheese made by microbial rennet from bacillus amyloliquefaciens: a comparison with commercial calf rennet. | miniature cheddar-type cheeses were produced using microbial rennet from bacillus amyloliquefaciens (milk-clotting enzyme [mce]) or calf rennet (car). with the exception of ph, there were no significant differences in gross composition between mce-cheese (mce-c) and car-cheese (car-c). the ph value of car-c was significantly higher than that of mce-c at 40 and 60 d of ripening. the total nitrogen content of the ph 4.6-soluble fraction obtained from mce-c was higher than that obtained from car-c. ... | 2014 | 24446932 |
| biocontrol of fusarium graminearum growth and deoxynivalenol production in wheat kernels with bacterial antagonists. | fusarium graminearum is the main causal pathogen affecting small-grain cereals, and it produces deoxynivalenol, a kind of mycotoxin, which displays a wide range of toxic effects in human and animals. bacterial strains isolated from peanut shells were investigated for their activities against f. graminearum by dual-culture plate and tip-culture assays. among them, twenty strains exhibited potent inhibition to the growth of f. graminearum, and the inhibition rates ranged from 41.41% to 54.55% in d ... | 2014 | 24441510 |
| statistical optimization of culture conditions for milk-clotting enzyme production by bacillus amyloliquefaciens using wheat bran-an agro-industry waste. | in order to improve the production of the milk-clotting enzyme under submerged fermentation, two statistical methods were applied to optimize the culture conditions of bacillus amyloliquefaciens d4 using wheat bran as nutrient source. first, initial ph, agitation speed, and fermentation time were shown to have significant effects on d4 enzyme production using the plackett-burman experimental design. subsequently, optimal conditions were obtained using the box-behnken method, which were as follow ... | 2013 | 24426157 |
| probiotic bacillus amyloliquefaciens sc06 prevents bacterial translocation in weaned mice. | probiotic is a preparation containing microorganisms that confers beneficial effect to the host. this work assessed whether oral administration of bacillus amyloliquefaciens sc06 (ba) could decrease bacterial translocation in weaned mice. weaned c57bl/6 were randomly allocated into three groups: group i as the control group, group ii were treated with 0.85 % nacl. group iii was administered with probiotic ba 1 × 10(9) cfu/day dissolved in 100 μl of 0.85 % nacl for 30 days. mice were then sacrifi ... | 2013 | 24426131 |
| characteristics of streptomyces griseus biofilms in continuous flow tubular reactors. | the purpose of this study was to investigate the feasibility of cultivating the biotechnologically important bacterium streptomyces griseus in single-species and mixed-species biofilms using a tubular biofilm reactor (tbr). streptomyces griseus biofilm development was found to be cyclical, starting with the initial adhesion and subsequent development of a visible biofilm after 24 h growth, followed by the complete detachment of the biofilm as a single mass, and ending with the re-colonisation of ... | 2014 | 24417230 |
| mutagenetic study of a novel inosine monophosphate dehydrogenase from bacillus amyloliquefaciens and its possible application in guanosine production. | in this study, the amino acid sequence of inosine monophosphate dehydrogenase (impdh) from a guanosine-overproducing strain bacillus amyloliquefaciens ta208 was found to be highly conserved comparing to its analogue in b. amyloliquefaciens fzb42, only with two substitutions of serine 166 to proline and glutamic acid 481 to lysine. to speculate on the effects of these variation sites, two reverse site-directed mutants p166s and k481e, as well as one deletion mutant impdh(δcbs), were characterised ... | 2014 | 26019494 |
| optimization of carboxymethylcellulase production from bacillus amyloliquefaciens ss35. | in this paper, we have attempted optimization of production of enzyme carboxymethylcellulase or endoglucanase from the bacterium bacillus amyloliquefaciens ss35. optimization has been carried out in two stages using statistical experimental design, viz. medium optimization and optimization of fermentation parameters. for medium optimization, plackett-burman design followed by central composite design (ccd) was used, while for optimization of fermentation parameters one-variable-at-a-time method ... | 2014 | 28324477 |
| comparison of the kinetics of lipopeptide production by bacillus amyloliquefaciens xz-173 in solid-state fermentation under isothermal and non-isothermal conditions. | this study aimed to compare the kinetics of lipopeptide production in solid-state fermentation (ssf) under isothermal and non-isothermal conditions. models based on the logistic, modified gompertz and luedeking-piret-like equations were developed to describe the time course of fermentation under different conditions. the experiments were conducted in 250 ml flasks and a 50 l fermenter. the results showed that the non-isothermal process had higher levels of product formation rate and substrate ut ... | 2014 | 24370986 |
| characterization of amylolysin, a novel lantibiotic from bacillus amyloliquefaciens ga1. | lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. they are capable to inhibit the growth of gram-positive bacteria, including listeria monocytogenes, staphylococcus aureus or bacillus cereus, the causative agents of food-borne diseases or nosocomial infections. lantibiotic biosynthetic machinery is encoded by gene cluster composed by a structural gene that codes for a pre-lantibiotic peptide and other genes involved in ... | 2013 | 24349428 |
| design, synthesis and antibacterial activities of 5-(pyrazin-2-yl)-4h-1,2,4-triazole-3-thiol derivatives containing schiff base formation as fabh inhibitory. | a series of novel schiff base derivatives (h(1)-h(20)) containing pyrazine and triazole moiety have been designed and synthesized, and their biological activities were also evaluated as potential inhibitors of β-ketoacyl-acyl carrier protein synthase iii (fabh). these compounds were assayed for antibacterial activity against escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, bacillus subtilis and bacillus amyloliquefaciens and selected compounds among them were tested for their esc ... | 2014 | 24332628 |
| characterization of cellulolytic bacterial cultures grown in different substrates. | nine aerobic cellulolytic bacterial cultures were obtained from the leibniz institute dsmz-german collection of microorganisms and cell culture (dsmz) and the american type culture collection (atcc). the objectives of this study were to characterize the cellulolytic bacteria and to determine the optimum moisture ratio required for solid state fermentation (ssf) of palm kernel cake (pkc). the bacteria cultures were grown on reconstituted nutrient broth, incubated at 30°c and agitated at 200 rpm. ... | 2013 | 24319380 |
| cloning of a family 11 xylanase gene from bacillus amyloliquefaciens ch51 isolated from cheonggukjang. | bacillus amyloliquefaciens ch51, an isolate from cheonggukjang, korean fermented soyfood, secretes several enzymes into culture medium. a gene encoding 19 kda xylanase was cloned by pcr. sequencing showed that the gene encoded a glycohydrolase family 11 xylanase and named xyna. xynahis, xyna with additional codons for his-tag, was overexpressed in escherichia coli bl21(de3) using pet-26b(+). xynahis was purified using histrap affinity column. km and vmax of xynahis were 0.363 mg/ml and 701.1 μmo ... | 2012 | 24293733 |
| antimicrobial activity of bacillus amyloliquefaciens ant1 toward pathogenic bacteria and mold: effects on biofilm formation. | the intensive use and misuse of antibiotics over the last decades have generated a strong selective pressure for the emergence of multi-resistant strains and nosocomial infections. biofilm has been demonstrated as a key parameter in spreading infections, especially in hospitals and healthcare units. therefore, the development of novel anti-biofilm drugs is actually of the upmost importance. here, the antimicrobial and antibiofilm activities toward pathogenic microorganisms of a set of non-riboso ... | 2013 | 26783071 |
| a new pgpr co-inoculated with bradyrhizobium japonicum enhances soybean nodulation. | a new pgpr (plant growth promoting rhizobacteria) strain was isolated from soybean seeds and the bacterial mechanisms related to plant growth promotion were evaluated and characterized. isolates were genotypically compared and identified by amplification of partial sequences of 16s dnar as bacillus amyloliquefaciens strain ll2012. isolates were grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, enzymatic activities, phosphate solubilization, siderophores and phyt ... | 2014 | 24280513 |
| characterization of antagonistic-potential of two bacillus strains and their biocontrol activity against rhizoctonia solani in tomato. | to investigate the biocontrol mechanism of two antagonistic bacillus strains (bacillus subtilis mb14 and bacillus amyloliquefaciens mb101), three in vitro antagonism assays were screened and the results were concluded that both strains inhibited rhizoctonia solani growth in a similar manner by dual culture assay, but the maximum percent of inhibition only resulted with mb101 by volatile and diffusible metabolite assays. moreover, cell free supernatant (cfs) of mb101 also showed significant (p > ... | 2015 | 24277414 |
| in vitro and in vivo characterization of plant growth promoting bacillus strains isolated from extreme environments of eastern algeria. | this report is to our knowledge the first to study plant growth promotion and biocontrol characteristics of bacillus isolates from extreme environments of eastern algeria. seven isolates of 14 (50 %) were screened for their ability to inhibit growth of some phytopathogenic fungi on pda and some roots exudates. the bacteria identification based on 16s r-rna and gyrase-a gene sequence analysis showed that 71 % of the screened isolates belonged to bacillus amyloliquefaciens and the rest were closel ... | 2014 | 24258791 |