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chemical and botanical characterization of chilean propolis and biological activity on cariogenic bacteria streptococcus mutans and streptococcus sobrinus.propolis is a non-toxic natural substance with multiple pharmacological properties including anti-cancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. the aim of this study was to determine the chemical and botanical characterization of chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria streptococcus mutans and streptococcus sobrinus. twenty propolis samples were obtained from beekeeping producers from the ...201324294257
frequency, biofilm formation and acid susceptibility of streptococcus mutans and streptococcus sobrinus in saliva of preschool children with different levels of caries activity.one of the causative factors in development of dental caries is microorganisms. two species of mutans streptococci including streptococcus mutans and streptococcus sobrinus are associated with dental caries in human beings. the aim of this study was to investigate the frequency of s. mutans and s. sobrinus in saliva of children with different caries activity and ability to form biofilm and acid susceptibility of these microorganisms.201324130577
[the relationship between streptococcus sobrinus and rampant caries in children].to explore the relations between streptococcus sobrinus (s. sobrinus) and rampant caries in children.201324100904
streptococcus mutans and streptococcus sobrinus detection by polymerase chain reaction and their relation to dental caries in 12 and 15 year-old schoolchildren in valencia (spain).a cross-sectional study was carried out to determine the prevalence of streptococcus mutans and streptococcus sobrinus and the association of the two in a random sample (n=614) of the child population of the region of valencia (spain). saliva samples were analyzed by the quantitative polymerase chain reaction (pcr) method to study the relation of these bacteria to caries prevalence and the dmft index. the prevalence of s. mutans was 35.4% at age 12 and 22.9% at age 15, that of s. sobrinus 18.9% ...201323722138
adhesion of streptococci to various orthodontic composite resins.this investigation aimed to determine quantitatively the adhesion of streptococcus mutans and streptococcus sobrinus to orthodontic composite resins that were tested simultaneously using radio-markers.201323441799
antimicrobial activity of the synthetic peptide lys-a1 against oral streptococci.the peptide lys-[trp(6)]-hy-a1 (lys-a1) is a synthetic derivative of the peptide hy-a1, initially isolated from the frog species hypsiboas albopunctatus. according to previous research, it is a molecule with broad antimicrobial activity. the objective of this study was to evaluate the antimicrobial activity of the synthetic peptide lys-a1 (kifgaiwplalgalknlik-nh2) on the planktonic and biofilm growth of oral bacteria. the methods used to evaluate antimicrobial activity include the following: det ...201323340019
recovery of mutans streptococci on msb, sb-20 and sb-20m agar media.the recovery of mutans streptococci in saliva and dental biofilm samples depends, in part, on the culture medium used. in this study, we compared (i) the culture media sucrose-bacitracin agar (sb-20), modified sb-20 (sb-20m) and mitis salivarius bacitracin agar (msb) in the count of colony forming units (cfu) of mutans streptococci and (ii) in the morphological and biochemical differentiation between streptococcus mutans and streptococcus sobrinus.201323149153
effects of withania somnifera on the growth and virulence properties of streptococcus mutans and streptococcus sobrinus at sub-mic levels.the aim of this study was to evaluate the effect of the methanol extract of withania somnifera (mew) on the growth and virulence properties of streptococcus mutans and streptococcus sobrinus at sub-minimum inhibitory concentration (mic) levels and to identify the main components of mew. first, antibacterial activity of mew against oral bacteria was determined using a micro-dilution method. then, the effect of mew on the growth of s. mutans and s. sobrinus was investigated at sub-mic levels. to t ...201323142795
aciduric microbiota and mutans streptococci in severe and recurrent severe early childhood caries.severe early childhood caries (ecc) results from bacterial acid production in an acidic environment. the purpose of this study was to determine streptococcus mutans, streptococcus sobrinus, and acid-tolerant counts in severe early childhood caries.201722583872
children with severe early childhood caries: pilot study examining mutans streptococci genotypic strains after full-mouth caries restorative therapy.genotypic strains of mutans streptococci (ms) may vary in important virulence properties and be differentially affected by specific components of full-mouth caries restorative therapy. the purpose of this pilot study was to identify mutans streptococci strains that predominate following caries restorative therapy.201722583870
ap-pcr detection of streptococcus mutans and streptococcus sobrinus in caries-free and caries-active subjects.the aim of the study was to identify the number and distribution of genotypes of streptococcus mutans (s. mutans) and streptococcus sobrinus (s. sobrinus) isolated from caries-free and caries-active subjects. non-stimulated saliva, buccal smooth surface of the right upper teeth, fissures of sound occlusal surface, and carious surface were sampled from 7 caries-free and 7 caries-active (dmft ≥ 6) students aged 22-24 years. s. mutans and s. sobrinus were isolated using chelex-100 and primarily ide ...201222407567
cotrimoxazole prophylaxis specifically selects for cotrimoxazole resistance in streptococcus mutans and streptococcus sobrinus with varied polymorphisms in the target genes fola and folp.the selection of antibiotic resistance by cotrimoxazole prophylaxis was evaluated, and we characterized the mechanism of cotrimoxazole resistance in streptococcus mutans and streptococcus sobrinus. in vitro susceptibility to six antibiotics was evaluated on 64 mutans streptococci group (msg) isolates from a cotrimoxazole prophylaxis group and compared to 84 msg isolates from a nonprophylaxis group. the fola and folp genes were sequenced and compared with reference sequences at ncbi. only resista ...201222315614
caries prevalence associated with streptococcus mutans and streptococcus sobrinus in japanese schoolchildren.streptococcus mutans and streptococcus sobrinus are known to be associated with dental caries in humans.201222225789
antibacterial peptides: opportunities for the prevention and treatment of dental caries.dental caries is a multifactorial disease that is a growing and costly global health concern. the onset of disease is a consequence of an ecological imbalance within the dental plaque biofilm that favors specific acidogenic and aciduric caries pathogens, namely streptococcus mutans and streptococcus sobrinus. it is now recognized by the scientific and medical community that it is neither possible nor desirable to totally eliminate dental plaque. conversely, the chemical biocides most commonly us ...201126781572
inhibitory effects of ellagic acid on glucosyltransferases from mutans streptococci.ellagic acid and its derivatives were found to abrogate the enzymatic activity of the glucosyltransferases (gtases) of mutans streptococci. these compounds preferentially inhibited the water-soluble glucan synthesis by gtase of streptococcus mutans mt8148 and inhibited the water-insoluble glucan synthesis by gtase of streptococcus sobrinus 6715. this inhibition resulted in a reduction of cellular adherence of mutans streptococci to a glass surface.199227286205
penetration of streptococcus sobrinus and streptococcus sanguinis into dental enamel.the aim of this pilot study was to assess the difference in virulence of acidogenic and aciduric oral streptococci in an in vitro caries model using their penetration depths into dental enamel. 30 caries-free extracted molars from 11- to 16-year-olds were cleaned ultrasonically for 1 min with de-ionized water and, after air-drying, embedded in epoxy resin. after 8-h of setting at room temperature, the specimens were ground on the buccal side with sic-paper 1200 (particle size 13-16 μm). enamel w ...201525805186
galla chinensis compounds remineralize enamel caries lesions in a rat model.to investigate the effect of galla chinensis chemical compounds on enamel caries remineralization in rats.201627111668
effect of milk and yogurt on streptococcus sobrinus counts and caries score in rats.an anti-cariogenic diet containing probiotics can be effective in caries prevention. this animal study compared the effects of milk and yogurt on streptococcus sobrinus counts and caries score.201726759594
effect of rhus coriaria l. water extract on five common oral bacteria and bacterial biofilm formation on orthodontic wire.recently, use of herbal medicine and plant extracts as a substitute for commercially available chemical drugs for control of infectious diseases such as dental caries and periodontal disease has become increasingly popular. the present study was aimed to evaluate the effect of rhus coriaria l. water extract on five common oral bacteria and bacterial biofilm formation on orthodontic wire.201425802711
bacterial profiles of saliva in relation to diet, lifestyle factors, and socioeconomic status.the bacterial profile of saliva is composed of bacteria from different oral surfaces. the objective of this study was to determine whether different diet intake, lifestyle, or socioeconomic status is associated with characteristic bacterial saliva profiles.201424765243
effect of silver ion coating of fixed orthodontic retainers on the growth of oral pathogenic bacteria.titanium and stainless steel wires used for retainers in orthodontic procedures were coated with ag ions and the effects of the coating on common oral pathogens and their pathogenicity were investigated. two species of cariogenic and three species of periodontopathic bacteria were assessed. biofilms of streptococcus sobrinus and two vsc gases produced by p. gingivalis were also examined. ag ion-coated wires showed marked antibacterial activities compared with uncoated wires; in most cases, the d ...201424614999
genotypic diversity and virulence traits of streptococcus sobrinus isolated from caries-free children and children suffering severe early childhood caries.to evaluate the genotypic diversity and some virulence traits of streptococcus sobrinus (s. sobrinus) isolated from caries-free children and children suffering severe early childhood caries (secc).201323878828
evaluation of cariogenic bacteria.the evaluation of mutans streptococci (ms) is one of the index for caries risk. dentocult(tm) and crt(tm) are commercial kits to detect and evaluate ms, conveniently. however, the evaluation of ms has also been carried out simply using an instruction manual. but the instruction manual is not easy to use for evaluation of ms. the aim of this study was to examine the utility of modified mitis-salivalius bacitracin (msb) agar medium compared with msb agar medium and commercial kits, and to establis ...200719212495
inhibition of glucan synthesis by casein polymers crosslinked by glutaraldehyde.glutaraldehyde-crosslinked α-casein polymers with molecular masses of 1-5×10(5) were found to inhibit adhesive insoluble glucan formation catalyzed by glucan synthases from streptococcus sobrinus b13, a cariogenic oral bacterium. of the three subcomponents of the glucan synthases tested, primer-dependent insoluble glucan synthase was inhibited specifically by the m r~2.7×10(5) polymer at a concentration of 1.4-8.5 μg/ml. the polymer was also active in inhibiting the artificial plaque formation b ...199827393366
the oral bacterial microbiome of occlusal surfaces in children and its association with diet and caries.dental caries is the most prevalent disease in humans globally. efforts to control it have been invigorated by an increasing knowledge of the oral microbiome composition. this study aimed to evaluate the bacterial diversity in occlusal biofilms and its relationship with clinical surface diagnosis and dietary habits. anamneses were recorded from thirteen 12-year-old children. biofilm samples collected from occlusal surfaces of 46 permanent second molars were analyzed by 16s rrna amplicon sequenci ...201728678838
chemical composition, antibacterial, schistosomicidal, and cytotoxic activities of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae).we have investigated the chemical composition and the antibacterial activity of the essential oil of dysphania ambrosioides (l.) mosyakin & clemants (chenopodiaceae) (da-eo) against a representative panel of cariogenic bacteria. we have also assessed the in vitro schistosomicidal effects of da-eo on schistosoma mansoni and its cytotoxicity to gm07492-a cells in vitro. gas chromatography (gc) and gas chromatography-mass spectrometry (gc/ms) revealed that the monoterpenes cis-piperitone oxide (35. ...201728504841
oral microbial profiles of individuals with different levels of sugar intake.the aim was to compare the oral microbial profiles in young adults with an intake of free sugars above or below the current recommendations by the who for sugar consumption. seventy subjects completed a quantitative food frequency questionnaire to establish the proportion of free sugars in relation to the total energy intake (% e). subjects with <5% e (n = 30) formed the low-sugar group, while those with ≥5% e (n = 40) were regarded as reference group. saliva and plaque samples were analyzed by ...201728839520
effect of arginine on the growth and biofilm formation of oral bacteria.alkali production via arginine deiminase system (ads) of oral bacteria plays a significant role in oral ecology, ph homeostasis and inhibition of dental caries. ads activity in dental plaque varies greatly between individuals, which may profoundly affect their susceptibility to caries.201728668766
antimicrobial and biological activity of leachate from light curable pulp capping materials.characterization of a number of pulp capping materials and assessment of the leachate for elemental composition, antimicrobial activity and cell proliferation and expression.201728645637
characterization and antimicrobial efficacy of portland cement impregnated with silver nanoparticles.this study investigated the effects of silver nanoparticle (sn) loading into hydraulic calcium silicate-based portland cement on its mechanical, antibacterial behavior and biocompatibility as a novel dental bone substitute.201728680554
salivary density of streptococcus mutans and streptococcus sobrinus and dental caries in children and adolescents with down syndrome.to assess and compare dental caries experience and salivary s. mutans, s. sobrinus, and streptococci counts between groups of down syndrome and non-down syndrome children and adolescents.201728678943
improved method for rapid and accurate isolation and identification of streptococcus mutans and streptococcus sobrinus from human plaque samples.mutans streptococci (ms), specifically streptococcus mutans (sm) and streptococcus sobrinus (ss), are bacterial species frequently targeted for investigation due to their role in the etiology of dental caries. differentiation of s. mutans and s. sobrinus is an essential part of exploring the role of these organisms in disease progression and the impact of the presence of either/both on a subject's caries experience. of vital importance to the study of these organisms is an identification protoco ...201728606792
in-vitro efficacy of different morphology zinc oxide nanopowders on streptococcus sobrinus and streptococcus mutans.zno with two different morphologies were used to study the inhibition of streptococcus sobrinus and streptococcus mutans which are closely associated with tooth cavity. rod-like shaped zno-a and plate-like shaped zno-b were produced using a zinc boiling furnace. the nanopowders were characterized using energy filtered transmission electron microscopy (eftem), x-ray diffraction (xrd), photoluminescence (pl) spectroscopy, raman spectroscopy and dynamic light scattering (dls) to confirm the propert ...201728576061
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