| molecular adaptability of carp myosin: a study of some physico-chemical properties and their comparison with those of rabbit myosin. | during thermal inactivation, the addition of as low as m urea resulted in the reduction of delta g identical to barrier of the inactivation of carp myosin ca2+-atpase, whereas that of rabbit myosin remained unaffected. in the absence of urea, a four-hour incubation of carp myosin was accompanied by the release of light chains at 30 degrees c, a value 10 degrees c lower than that for rabbit myosin. electron micrographs revealed that carp myosin forms artificial thick filaments, which were uniform ... | 1979 | 93883 |
| glucose 6-phosphatase activity in isolated and cultured hepatocytes from control and diethylnitrosamine-treated rats. a biochemical and cytochemical approach [proceedings]. | | 1979 | 93898 |
| [separation of cellular and viral dna polymerase from oncornavirus infected chicken cells]. | rna-directed dna polymerase was isolated from the liver, spleen, and myeloblasts of chickens that had been infected with virus of avian myeloblastosis. the enzyme was chromatographically purified from the myeloblasts and brought to about 1,000-fold concentration. the method consisted in cell fractionation, lysis of the microsomal fraction, chromatography on sephadex g-200 and phosphocellulose, as well as ultracentrifugation in the glycerol gradient. the cellular dna polymerases alpha and beta we ... | 1979 | 93956 |
| immunological and serological data concerning human histocompatibility antigens (hla) and alpha 1-microglobulin co-purified from urine. | serologically active glycoproteins (hla) purified from urine contained only about 4% hla antigens co-purified with alpha 1-microglobulin. the immunization of rabbits with the serologically active product gave rise to an antiserum containing two kinds of antibodies: anti-hla-a9 and anti-alpha 1-microglobulin. the anti-hla-a9 antibodies were detected by the lymphocytotoxicity and indirect immunofluorescence techniques against peripheral blood lymphocytes and cultured lymphoid cell lines. anti-alph ... | 1979 | 93973 |
| bleomycin pharmacokinetics in man. ii. intracavitary administration. | | 1979 | 93985 |
| pharmacokinetics of bleomycin in man. iii. bleomycin 57co vs bleomycin. | | 1979 | 93987 |
| liposome-incorporated enzymes: studies on amylase. | alpha-amylase (ec 3.2.1.1) from hog pancreas was incorporated into artificial phosphatidylcholine and phosphatidylserine liposomes. to kinetically follow the enzyme-catalyzed hydrolysis of amylose the liposome-bound amylase was incubated in a medium containing amylose-iodine substrate. the reaction was studied at different concentrations of amylose and at different ionic strengths. activation of amylase incorporated into liposomes by chloride ions varies with the type of phospholipid utilized to ... | 1979 | 93989 |
| common enterobacterial antigen and onpg test in the taxonomy of the genus yersinia. | the detection of the common enterobacterial antigen (kunin) in strains of y. pestis, y. pseudotuberculosis and y. enterotocolitica has brought an additional reason for separating them from the pasteurella and for their inclusion in the family enterobacteriaceae. yersinia that give a positive onpg test lack true beta-galactoridase, except when they harbour a lactose plasmid. | 1979 | 94001 |
| activity of retinoids against benzo(a)pyrene-induced hyperplasia in mouse prostate organ cultures. | | 1979 | 94011 |
| [hemolysis inhibition reaction for titrating antibodies to staphylococcus aureus alpha-toxin in small volumes of blood serum]. | | 1979 | 94134 |
| intermolecular base-paired interaction between complementary sequences present near the 3' ends of 5s rrna and 18s (16s) rrna might be involved in the reversible association of ribosomal subunits. | highly conserved sequences present at an identical position near the 3' ends of eukaryotic and prokaryotic 5s rrnas are complementary to the 5' strand of the m2(6)a hairpin structure near the 3' ends of 18s rrna and 16s rrna, respectively. the extent of base-pairing and the calculated stabilities of the hybrids that can be constructed between 5s rrnas and the small ribosomal subunit rnas are greater than most, if not all, rna-rna interactions that have been implicated in protein synthesis. the e ... | 1979 | 94160 |
| prevalence of hepatitis b virus and primary hepatocellular carcinoma in asia. | | 1979 | 94184 |
| antifetal and tumor-specific reactivity in human osteosarcoma. | | 1979 | 94187 |
| changes in ovarian activity during hepatocarcinogenesis by n-2-fluorenylacetamide: role of alpha-fetoprotein. | during chemical hepatocarcinogenesis by n-2-fluorenylacetamide, the hormonal status of female sprague dawley rats is largely modified. ovaries present a reduced activity which is evidenced by a decreased number of follicles and corpora lutea. the level of progesterone in n-2-fluorenylacetamide treated rats is low and agrees with the lack of corpora lutea. conversely, estradiol level remains normal. this fact may be explained by the presence of alpha-fetoprotein, a carcino-embryonic protein which ... | 1979 | 94191 |
| [immune response to artificial antigens]. | | 1979 | 94200 |
| an in vitro study on the effect of benzoyl peroxide 20%, gentamicin and phenoxyethanol on staphylococcus aureus, pseudomonas aeruginosa and proteus species. | | 1979 | 94224 |
| on the mechanism of hbfp stain and an analysis of heterogeneous nuclei in rat hepatocytes. | haematoxylin-basic fuchsin-picric acid (hbfp) technique characterises two varieties of nuclear population in the rat hepatocytes. hbfp technique is capricious and the differentiation step should be controlled stringently; ethanolic picric acid, therefore, is recommended as a differentiation fluid. on the basis of controls treated with (1) ribonuclease (rnase), (2) deoxyribonuclease (dnase), (3) bouins fluid or (4) protease, this study has determined that dna-associated protein(s) and some dna ma ... | 1979 | 94226 |
| cross-reactions between staphylococcus aureus and fifteen other bacterial species. | fifty-five different antigens were demonstrated in an antigen preparation obtained by sonication of a protein a-free strain of staphylococcus aureus, using crossed immunoelectrophoresis and antiserum obtained from rabbits. the antigens were characterized by absorption experiments with formalin-killed bacterial cells, temperature resistance and protein and polysaccharide staining. one of the antigens showed reaction of identity with a preparation of teichoic acid from s. aureus. using this refere ... | 1979 | 94234 |
| mycoplasma pneumonia presenting as meningoencephalitis and hemolytic anemia. | simultaneous occurrence of cold agglutinin hemolytic anemia and central nervous system disease due to mycoplasma pneumonia is extremely rare. a patient is described who initially presented with severe hemolysis and diffuse neurologic dysfunction which appeared during the period of roentgenographic resolution of her pneumonia. spectrum, pathogenesis, and antimicrobial treatment of the neurologic complications are reviewed. | 1979 | 94244 |
| regulatory sequences involved in the promotion and termination of rna transcription. | | 1979 | 94251 |
| c- and g-banding patterns and chromosomal morphology of some breeds of australian cattle. | a cytogenetical study using metaphase chromosomes from cultured lymphocytes, was made of 2 banteng (bibos banteng) steers and 218 bulls representing 13 purebreeds (bos taurus type, bos indicus type and sanga) and 7 cross-breeds. studies were made of photographic karyotypes of giemsa stained and c-banded chromosomes of bulls of each breed and of b-banded chromosomes from 3 breeds of bos indicus and one cross-breed australian friesian sahiwal) cattle. the relative lengths of chromosomes of bos tau ... | 1979 | 94258 |
| natural plant enzyme inhibitors: part vii--alpha amylase inhibitors & amylases in plant tubers. | | 1979 | 94300 |
| the cyclic amp-dependent initiation of dna synthesis by t51b rat liver epithelioid cells. | the brief rise in the cellular cyclic amp content which occurs late in the prereplicative phases of rat hepatocytes in vivo and t51b rat liver epitheloid cells in vitro seems to be necessary for the initiation of dna synthesis. thus, the extracellular calcium-deprivation in t51b rat liver cells in culture which induces a late g-1 block is rapidly reversible (cells surge into s phase within one hour) either by creating a cyclic amp surge by the addition of calcium or 3-isobutyl-1-methyl xanthine ... | 1979 | 94328 |
| simultaneous analysis of phenylglycols and phenylethanols in human urine by gas chromatography-mass spectrometry. | | 1979 | 94330 |
| detection of polymorphism in balb/c leukemia viruses with mouse antisera. | antisera produced in mice recognize primarily type-specific antigenic determinants on both the major core protein, p30, and the major envelope proteins, gp70 and p15(e), of the endogenous leukemia viruses (mulv) of balb/c mice. three different mouse sera were investigated in detail. (i) antisera prepared in c57bl/6 mice against the akr leukemia k36 reacted with the gp70, p15(e), and p30 proteins of mulv. certain pools of the c57bl/6 anti-akr k36 serum contained antibodies which serologically dis ... | 1979 | 94360 |
| [a study of substrate in antinuclear antibody technique (author's transl)]. | | 1979 | 94362 |
| effect of 6-hydroxydopamine on baroreceptor-heart rate and nasopharyngeal reflexes of the rabbit. | | 1979 | 94399 |
| disopyramide plasma and myocardial tissue concentrations as they relate to antiarrhythmic activity. | antiarrhythmic concentrations of disopyramide in canine plasma and myocardium were determined by gas chromatography. ventricular tachycardia was incuded in anesthetized dogs by the intravenous administration of ouabain. disopyramide phosphate was then administered by a two-stage continuous infusion method. a rapid infusion of disopyramide (9.08 mg/kg/hr) was administered for 30 min, followed by a slow infusion (2.18 mg/kg/hr) to maintain steady-state plasma levels of 1.98-2.21, mean +/- sem = 2. ... | 1979 | 94410 |
| cardiotonic effects of anthopleurin-a, a polypeptide from a sea anemone. | the positive inotropic effect of anthopleurin-a (ap-a) was studied in vitro on isolated cat heart papillary muscles and in vivo in anesthetized and conscious dogs. in vitro, in low ca2+ solution (1.27 mm), ap-a increased the force of contractions of isolated cat heart papillary muscles at concentrations from 0.2 x 10(-8) m and higher; on a molar basis, ap-a was more than 200 times as potent as digoxin and on a weight basis, 33 times as potent. in vivo in anesthetized dogs, ap-a at 0.2 microgram/ ... | 1979 | 94413 |
| action of substance p on neurotico-hypertensive rats. | the action of an eledoisin-hexapeptide analogue (eh) upon learning and memorising processes of 48 male wistar laboratory rats aged between 5 and 6 months was studied and is reported in this paper. the animals suffered from neurogenic hypertension which had been experimentally induced by applying emotional stress. a comparison between the action of eh (lys-phe-ile-gly-leu-metnh2) and that of substance p (arg-pro-lys-pro-gln-gln-phe-phe-gly-leu-metnh2) on conditioned-reflex learning in the intact ... | 1979 | 94444 |
| [prognostic value of alpha-foetoprotein in fulminant hepatitis (author's transl)]. | serum concentrations of alpha-foetoprotein (afp) were measured sequentially by radioimmunoassay in 32 patients with fulminant hepatitis and coma, 22 of whom died. levels were significantly elevated in 100% of patients who survived and in 7 out of 22 patients (38.5%) who died (p = less than 0.005). in the survivors the rise of afp levels was found early after the development of coma and subsequently in all serum samples obtained during acute phase of illness. in 2 of 7 fatal cases who had raised ... | 1979 | 94446 |
| alcoholic hepatitis: clinical, morphologic, pathogenic, and therapeutic aspects. | | 1979 | 94448 |
| [repeated contamination of an antiseptic solution by mycobacterium chelonei]. | | 1979 | 94449 |
| effects of subminimal inhibitory concentrations of ampicillin, chloramphenicol, and nitrofurantoin on the attachment of escherichia coli to human uroepithelial cells in vitro. | an in vitro system with epithelial cells of the human urinary tract and escherichia coli isolated from the urine of pateints with acute pyelonephritis was used for tests of the effect of antibacterial agents on bacterial adhesion. e. coli treated with subminimal inhibitory amounts of ampicillin attached less than untreated control bacteria. subminimal inhibitory amounts of chloramphenicol and nitrofurantoin had no effect on adhesion of the 15 strains of e. coli tested. the combination of bacteri ... | 1979 | 94452 |
| the humoral immune response to alpha, beta and gamma crystallins of the human lens. | by radioimmunoassay the occurrence of antibodies against alpha, beta and gamma crystallins was examined in sera from groups of patients with clear lenses, cataracts or lens injuries. in about half of the sera from each group low anti alpha crystallin activity was found. no significant difference in antibody activity was found between the groups. antibody activity against beta or gamma crystallins was not detected in any of the sera. four patients with lens damage were followed for 2 years; two ... | 1979 | 94463 |
| [immunofluorescent diagnosis of entamoeba histolytica trophocoites in preserved stool specimens of patients (author's transl)]. | a fixative and examination technique is described for identification of entamoeba histolytica trophocoites in faeces with the aid of the indirect immunofluorescence technique. magnaforms in bloody-dysenteric specimens and minutaforms in spontaneous or saline purged specimens give equally good fluorescence. the specifity of the rabbit antiserum against axenically grown e. hist. is good: there is strong positive reaction only with trophocoites of e. hist. weak crossreactions are encountered with t ... | 1979 | 94475 |
| studies on the nature of the type specific antigen of erysipelothrix rhusiopathiae. | | 1979 | 94508 |
| removal of dna from rna by chromatography on acetylated n-[n'-(m-dihydroxylborylphenyl)succinamyl]aminoethyl cellulose. | | 1979 | 94514 |
| influence of substance p on mesenteric hemodynamics in the pig. | | 1979 | 94532 |
| [localization of sacral spinal neurons innervating the perineal muscles--a hrp study in cat (author's transl)]. | | 1979 | 94555 |
| neuropeptides and pain: neurosurgical implications. | | 1979 | 94557 |
| cocultivation as a tool for the detection of oncoviruses in childhood leukemia. | | 1979 | 94580 |
| simian rotavirus sa-11 plaque formation in the presence of trypsin. | incorporation of 5 micrograms of trpsin per ml of the overlay (eagle minimal essential medium-0.7% ionagar no. 2) was found to be necessary for plaque formation by simian rotavirus sa-11. plaques of 3 to 4 mm in diameter were produced in ma-104 cells after 5 days of incubation at 37 degrees c. plaque size was even larger (5 to 6 mm) in monolayers of african green monkey kidney cells. addition of diethyl-aminotheyl-dextran, protamine sulfate, or 5-bromodeoxyuridine to the trypsin-containing overl ... | 1979 | 94597 |
| lectins in diagnostic microbiology: use of wheat germ agglutinin for laboratory identification of neisseria gonorrhoeae. | a lectin slide agglutination test has been developed for the confirmatory identification of neisseria gonorrhoeae. with wheat germ lectin as an agglutinin, 164 of 165 clinical isolates of n. gonorrhoeae gave a 3 to 4+ reaction within 6 to 8 min. four gonococcal isolates, even though negative by the fluoresecent-antibody method, gave strong positive reactions with the wheat germ lectin. among 23 isolates of neisseria meningitidis tested, which included representatives of sero-groups a, b, c,d, x, ... | 1979 | 94598 |
| acute and chronic beta-receptor blockage with propranolol and the cardiovascular responses to intravenous sodium nitroprusside in the conscious rabbit. | sodium nitroprusside infused intravenously into conscious rabbits lowered blood pressure and raised heart rate and plasma noradrenaline. acute beta blockade with propranolol, 2 mg/kg/hr i.v. for 2 hr, increased resting plasma noradrenaline and did not change the hypotensive effects of nitroprusside or the rise in plasma noradrenaline but attenuated the increase in heart rate. chronic treatment with propranolol, 2 mg/kg twice daily for 3 weeks, did not modify the cardiovascular or reflex effects ... | 1979 | 94618 |
| nature of the central serotonin receptors mediating hypotension. | the stimulation of central serotonin receptors leading to a decrease in blood pressure is well documented, but the nature of these receptors is not well known. it was therefore decided to characterize such receptors by assessing the modifications in blood pressure and heart rate after the intracerebroventricular administration of several drugs to anesthetized cats. serotonin produced a dose-related decrease in blood pressure and heart rate, while parachloroamphetamine produced a slowly developin ... | 1979 | 94623 |
| identification of bacteroides fragilis by indirect immunofluorescence. | rabbit antiserum against live or heat-killed bacteroides fragilis nctc 9343 bacteria was titrated against hot phenol water-extracted polysaccharide antigens from five different species of the 'b. fragilis group' of bacteria using an enzyme immunoassay and shown to be specific for the b. fragilis nctc 9343 polysaccharide. when the antiserum was used in indirect immunofluorescence, 97.1% of 244 b. fragilis strains were correctly identified. only 8 of the other 312 bacteroides strains were stained ... | 1979 | 94651 |
| [the mechanism of transcription in procaryotes and eucaryotes (author's transl)]. | | 1979 | 94670 |
| [changes of the plasmatic correlations between t3, t4 and tsh in patients with hyperthroidism before and after treatment with synthetic antithyroid drugs]. | | 1979 | 94678 |
| sharing ia antigens between species. iii. ia specificities shared between mice and human beings. | certain mouse alloantisera have been found to detect immunologic cross-reactions between human and murine ia antigens. almost every anti-iaa, -iak and -iad serum tested exhibited such cross-reactions. sera prepared against the products of limited segments of the mouse i region and tested on human b cells revealed that anti-i-e/ck cross-reactions were more readily detectable than anti-i-a, b, jk cross-reactions. most of the mouse alloantisera were cytotoxic to bells from almost every individual t ... | 1979 | 94693 |
| androgen receptors in benign and malignant prostatic tissue. | a new procedure has been developed to purify prostatic cell nuclei and to achieve a high yield of nuclear material for study. this technique allowed the investigation of the enzyme 5-alpha-reductase and its relationship to the translocation of dihydrotestosterone and androgen receptors into the nucleus of the prostatic cell. the studies revealed that 5-alpha-reductase was localized chiefly to the stromal components of all types of prostatic tissue. although there is a higher concentration of dih ... | 1979 | 94714 |
| effect of nutrients on the toxicity of pesticides carbofuran and hexachlorocyclohexane to blue-green alga nostoc muscorum. | the effects of various levels of nutrients like potassium phosphate (dibasic), calcium nitrate, and calcium chloride individually and in combinations were studied on the toxicity of the commonly used pesticides carbofuran and hexachlorocyclohexane (hch) in growth medium to the n2-fixing blue-green alga nostoc muscorum. it was observed that all these chemicals had some effects on toxicity. the toxicity of both carbofuran and hch was reduced to some extent in the presence of higher concentrations ... | 1979 | 94720 |
| effect of ddt and gamma-hch on the biological properties of staphylococci. | the effect of ddt and gamma-hch in concentrations of 100, 50, and 10 micrograms on the biological properties of staphylococci was studied. both substances proved to be inhibitory to coagulation of rabbit plasma and to penicillinase production, causing depigmentation of the colonies. the morphological structure of cells is affected by these substances as well. the growth of the strain is not substantially influenced. gas chromatography proved that staphylococci do not participate in the ddt and g ... | 1979 | 94724 |
| hla types in corneal diseases. | evaluation of the results of hla typing of 187 patients grouped into herpetic keratitis (37), non-herpetic keratitis (43), keratoconus (42), endothelial dystrophy (23), stromal dystrophy (13), lues (5), and injuries (24), failed to show convincing deviations in any of the groups from a normal control series (2900 persons). yet, as for the herpes group, a rise in b5 must strongly be suspected. the data are presented and possible implications are discussed. | 1979 | 94740 |
| corneal endothelial permeability following storage in moist chamber and mk medium. | rabbit corneal endothelial permeability to sucrose and dextran was determined after storage in moist chamber or mk medium. when compared to fresh rabbit corneas there was no statistically significant difference in endothelial permeability to these two solutes after both types of storage for periods up to ten days. | 1979 | 94741 |
| fungal nucleic acids as interferon inducers. | nucleic acids isolated from the fungi aspergillus niger x11, piptoporus betulinus and ganoderma applanatum reduced the number of vaccinia virus plaques in chick embryo fibroblast (cef) tissue culture and when administered intravenously to white mice protected them against lethal infection with tick borne encephalitis virus strain k5 (tbe). in cef tissue culture the nucleic acids of the studied fungi were found to induce small but detectable amounts of a substance with the character of interferon ... | 1979 | 94746 |
| igm antibodies in rubella infections: absorption with staphylococcal protein a of sera examined by immunofluorescence and haemagglutination inhibition. | a similar frequency of positive results for rubella igm antibodies in the sera of rubella patients and persons contacting such patients was obtained by immunofluorescence after separation of igm is sucrose gradient and after absorption of the sera with staphylococcus aureus and immunoglobulin aggregates. compared to the immunofluorescence method, the haemagglutination method for rubella igm antibodies performed after absorption of the sera with s. aureus and reduction of immunoglobulins m with 2 ... | 1979 | 94747 |
| effect of allopurinol on pyrimidine metabolism in human white blood cells. role of the salvage pathway. | | 1979 | 94760 |
| the monodisperse cervical smear: quantitative analysis of cell dispersion and loss with enzymatic and chemical agents. | the proper utilization of flow-through instruments for the automatic detection of malignant cells in human cervical specimens requires that the cells be in the form of a monodisperse suspension. information regarding the degree of cell dispersion and of cell loss is therefore of critical importance in the evaluation of any procedure used to render cervical specimens monodispe automatic detection of malignant cells in human cervical specimens requires that the cells be in the form of a monodisper ... | 1979 | 94778 |
| quantitative study of the energy conversion in halobacterium halobium [proceedings]. | | 1979 | 94791 |
| effect of beta-blockers on sodium and potassium content of human erythrocytes. | recently, hyperpotassemia was reported in patients treated with propranolol, but the mechanism has not yet been delineated. we have investigated the effects of various beta-adrenergic blockers and of d-propranolol on the na+ and k+ distribution intra- and extracellularly in human erythrocytes. k+ loss and na+ gain by cells was demonstrated at drug concentrations of 10(-4) m or greater. d-propranolol was more effective than l-propranolol, whereas pindolol was ineffective. practolol increased na+ ... | 1979 | 94824 |
| [study of membrane proteins from microccus lysodeikticus using immunochemical methods]. | using immunoelectrophoresis, the antigenicity of various protein fractions of the micrococcus lysodeikticus membranes was evaluated. it was shown that both the peripheral and integral membrane proteins possess the antigenic determinants. the antiserum exhausted by the m. lysodeikticus mebranes loses its ability to interact with intergral proteins, which are not solubilized by triton x-100. it was thus assumed that the integral proteins are exposed on the membrane surface constantly or periodical ... | 1979 | 94839 |
| [physiology of microcirculation]. | | 1979 | 94861 |
| [effect of ass on platelet function in experimental dic]. | in thrombin-induced dic, acetylsalicylic acid (asa) prevents the strong initial fall in platelet count and the obturation of the microvasculature of the lung with platelet aggregates. during the dic reaction increasing inhibition of aggregability of circulating platelets against collagen and adp is observed. furthermore, asa prevents the increase in the plasma haemoglobin level caused by dic. | 1979 | 94874 |
| [effect of aggregation inducers and inhibitors on the functional properties and the ultrastructure of platelets]. | the influence of inductors and inhibitors of platelet aggregation on certain functional properties and structural peculiarities of platelets are investigated. inductors of aggregation, such as adp and thrombin, will cause the calcium binding to the platelet membrane to be diminished, whereas inhibitory substances of platelet aggregation will cause an increase of calcium binding. inductors increase the number of partially degranulated platelets. inhibitors, such as triphtazin and papaverin, inhib ... | 1979 | 94877 |
| [animal experimental studies on fibrinolysis with streptokinase]. | in contrast to rabbit blood plasma, in guinea pig and rat blood plasma activation of fibrinolysis by streptokinase is achieved after addition of human plasminogen or human plasma only. a simple experimental procedure for testing application forms of streptokinase in rats is described. fibrinolysis in vivo is more effective after subsequent administration of human plasma and streptokinase in rats than after administration of a mixture of human plasma and streptokinase (activator). | 1979 | 94886 |
| ia antigens in serum during different murine infections. | there exists in the mouse a family of i-region-controlled (ia) antigens which carry carbohydrate-defined determinants. these antigens appear in serum as glycolipids and seem to be actively secreted by antigen-activated t-cells. this paper describes the ability of selected viral, bacterial, and protozoal infections of mice to markedly alter the serum levels of these ia antigens. all the infectious agents examined induced substantial augmentation or suppression of serum ia concentrations or both. ... | 1979 | 94905 |
| separation of small dna and rna oligonucleotides by high-performance anion-exchange liquid chromatography. | small oligonucleotides from dna and rna have been separated according to their base composition by high-performance anion-exchange liquid chromatography on partisil-10 sax using triethylammonium acetate buffer as the eluent. fifteen of the 16 possible deoxydinucleoside monophosphates and all 16 dinucleoside monophosphates have been separated. all pairs of sequence isomers were all resolved. the 15 commercially available deoxydinucleotides were resolved into 13 fractions. a good resolution of deo ... | 1979 | 94913 |
| rapid isolation of carcinogen-bound dna and rna by hydroxyapatite chromatography. | carcinogen-bound dna and rna are conveniently isolated by solvent extraction and hydroxyapatite (hap) chromatography. tissue is suspended in 8 m urea-0.24 m sodium phosphate-1% sodium dodecyl sulfate-10 mm edta, ph 6.8 (mup-sds-edta) and extracted with chloroform-isoamyl alcohol-phenol (24:1:25; cip) to remove protein. rna and dna are separated by passing the aqueous solution through an hap column; rna is eluted with mup, dna with 0.48 m sodium phosphate, ph 6.8. examples presented are: (1) calf ... | 1979 | 94918 |
| [inhibition of rna synthesis in vitro by antibodies against mononucleotides]. | antibodies against purine nucleotides were obtained from rabbits immunized with conjugates of bovine serum albumine with amp or gmp. the antibodies purified by affinity chromatography on nucleoside monophosphate-human serum albumine-sepharose columns inhibited rna synthesis on native t4 phage dna by e. coli rna polymerase. the inhibition of transcription was due mainly to inhibition of the initiation stage of rna synthesis. | 1979 | 94937 |
| histochemical studies of mucosubstances and keratins of thymic corpuscles. | keratin and prekeratin have been visualized histochemically by testing the reactivity of disulfide groups in the thymic corpuscles of the sheep. rhesus monkey, rabbit and dog, and the reactivity of sulfhydryl groups in the sheep, rhesus monkey, rabbit and rat. ten to 50% of the thymic corpuscles showed keratinization by this method. acidic mucosubstances were visualized histochemically in the rhesus monkey, sheep and rat but not in the rabbit. staining for vic-glycol groups was prominent in all ... | 1979 | 94996 |
| [intracellular localization of alpha-fetoprotein and serum albumin in the central nervous system of the rat during fetal and postnatal development]. | the morphological localisation of alphafetoprotein (afp), serumalbumin (sa), transferrin and immunoglobulins (igg) has been studied in the developing central nervous system of the rat by immunocytochemical methods. evidence is presented of a highly selective staining for afp and sa, both proteins exhibiting the same topographical distribution. practically all the areas of the brain and the spinal cord are stained at a given moment of the developmental process. the labeling is cytoplasmic and in ... | 1979 | 95002 |
| [use of a new selective stain for demonstrating the vagal fibers in gastric surgery. experimental study]. | scalar concentrations (0.4%-0.2%-0.1%-0.05%) of methylene blue in a 7.02% ascorbic acid solution have been used in animals and humans for visualizing vagal branches at gastric level. these solutions constitute a simplified variation of lee's test and the most effective for determining vagal fibres proved to be the 0.1% concentration. | 1979 | 95092 |
| demonstration of a murine cell surface component with affinity for exogenous beta 2-microglobulin. | murine and human beta 2-microglobulin (beta 2m) bind to various types of mouse cells. the binding is saturable and displays a single association constant of about 1 x 10(9) liter/mol. the binding of beta 2m to splenocytes was not affected by a variety of metabolic inhibitors but was temperature-dependent. it is suggested that the beta 2m "receptor" exhibits a temperature-dependent conformational change since the "receptor", whether integrated into the membrane or solubilized by the detergent tri ... | 1979 | 95108 |
| tumor immunology in domestic animals. | | 1979 | 95155 |
| mechanisms of immunity in bacterial infections. | | 1979 | 95156 |
| visualization of mitochondrial coupling factor f1(atpase) by freeze-drying. | it is known that the negatively stained preparations of inner mitochondrial membrane display characteristic approximately 9 nm f1 (atpase) knobs projecting from the matrix surface. freeze-etch studies have reported the absence of such knobs from the "etched" surface of the inner mitochondrial membranes. we have demonstrated their presence on the surface of smp (submitochondrial particles) prepared by freeze-drying for transmission electron microscopy. this identification has been substantiated b ... | 1979 | 95168 |
| the dissociation and separation of bovine adenohypophysial cells. | bovine adenhypophysial tissue was dissociated by sequential enzymatic incubation in a continuous flow system. dispersed cells separated into discrete fractions after centrifugation in isopycnic bovine serum albumin gradients. the dispersed and separated cells were prepared for microscopic identification and differential counts by centrifugal cytology. radioimmunoassays for lh, fsh, tsh, and prl were used to corroborate the differential counts and determine the homogeneity of the fractions. the t ... | 1979 | 95171 |
| assessment of immunocytochemical techniques with particular reference to the mixed-aggregation immunocytochemical technique. | antibody-mediated assays are believed to be the most sensitive and specific techniques for antigen localization available to date, but they need extensive controls if specific and quantitative results are to be obtained. problems arise from both the antibody source and the antigen in the tissue preparation. specificity of the antibody is often difficult to prove in any direct or indirect antibody technique. when markers are coupled to antibody, binding and diffusion properties of the antibody ar ... | 1979 | 95185 |
| inhibition and stimulation of histamine release by oxatomide. | | 1979 | 95250 |
| spontaneous termination of reentry ventricular tachycardia in the late myocardial infarction period: an experimental study in the dog. | forty episodes of induced ventricular tachycardia in the late myocardial infarction period (4-6 days old) were analyzed in 12 dogs in an attempt to identify the possible mechanisms for the termination of reentry tachycardia. multiple epicardial and endocardial composite electrograms were recorded in and around the central ischemic zone of the infarction. during tachycardia, the earliest site of activation was identified in the epicardial surface of the border or normal zone immediately adjacent ... | 1979 | 95291 |
| induction of sporulation in bacillus brevis by peptide antibiotics. | | 1979 | 95355 |
| probing the program of gene expression utilized in early development. | somatic cell nuclei injected into amphibian oocytes enlarge, exchange protein with the surrounding cytoplasm, and actively synthesize rna for weeks. the message activity of the rnas synthesized within the oocytes can be detected as new proteins made a few days after the injection of somatic nucleic. the oocyte cytoplasm seems to reprogram the injected nuclei, allowing the expression only of those genes which are normally active in oocytes (de robertis and gurdon, 1977). genes which are unexpress ... | 1979 | 95388 |
| histochemical characteristics of the anal sacs of the cat. | the histochemistry of polysaccharides and proteins and the distribution of rna were investigated in the glands and mature secretion of the anal sacs of the cat. glycogen was extremely abundant in the epithelial lining of the lumen and efferent duct of the anal sac and also in ducts of sebaceous glands of the sacs constantly formed in the place of degenerating acini. little glycogen was found in the unchanged sebaceous acini. the apocrine glands of the anal sacs were substantially richer in glyco ... | 1979 | 95391 |
| dynamic inorganic ion-protein interactions in structural organization of dna of living cell nuclei. | staining polarization optical techniques showed differences in the structural organization of dna of chromatin in interphase nuclei and in mitotic chromosomes. the dna was non-birefringent in intact interphase cell nuclei, but birefringent in chromosomes and in isolated nuclei incubated in a physiological electrolyte solution. the birefringence of dna appears to be related to an unfolding of dna filaments induced by free cations and to the oriented binding of dye molecules to dna phosphates. we ... | 1979 | 95394 |
| a case of a needle stick. | | 1979 | 95411 |
| observations of vitamin e in experimental oxygen-induced retinopathy. | retrolental fibroplasia (rlf) is a proliferative retinopathy observed in premature infants. its relationship to oxygen has led to both clinical and laboratory research into the potential benefits of the antioxidant tocopherol in this disorder. although the clinical trials to date have only suggested a beneficial result, studies in the kitten model demonstrate two significant actions of tocopherol in protecting against this retinopathy, only one of which is predicted by theory. these data support ... | 1979 | 95420 |
| human t cell subpopulations as defined by fc receptors. | | 1979 | 95424 |
| tumor markers for ovarian cancer. | the literature on tumor distinctive markers in ovarian cancer has been reviewed. various immunological and biochemical approaches have been attempted for the diagnosis and management of patients with ovarian cancer. the complex spectrum of antigens that can be detected in human ovarian cancer consists of several tumor-associated antigens, fetal or carcinoembryonic antigens, carcinoplacental markers, and normal tissue antigens. we have described and partially characterized two ovarian tumor-assoc ... | 1979 | 95453 |
| computer-controlled double-beam scanning microspectrophotometry for rapid microscopic image reconstructions. | a method for automated collection of various specific data from an entire microscopical preparation and their quantitative evaluation is described. its application to the study of neuronal connections is discussed in some detail. brain sections are scanned using a computer-controlled microscope for reflectance, fluorescences or absorbance signals. two illuminating beams are used, one of them being amplitude modulated. by means of a synchronous detection the two signals are recorded simultaneousl ... | 1979 | 95455 |
| preparation of monospecific subtyping reagents for hepatitis b surface antigen (hbsag). | | 1979 | 95457 |
| [replacement solutions and hemodilution]. | by definition, haemodilution involves the use of filling solutions, but in this technique the role of different solutions has yet to be clearly defined. amongst the criteria of choice used at the present time, two would seem to be essential: good tolerance, limiting the risks of complication and side effects to an absolute minimum; and a sufficiently long intravascular half-life ensuring good control of blood volume. on this basis, amongst the crystalloids ringer lactate offers definite advantag ... | 1979 | 95461 |
| in vivo and in vitro reactions to antigens of haemophilus influenzae in bronchial obstruction. | the frequency of precipitating antibody to heat-labile (h(1--2) and heat-stable (hcw and hcf) antigens of haemophilus influenzae was determined in patients with asthma, chronic bronchitis, cystic fibrosis and bronchiectasis and compared with that in a control group. this showed that the immune response of asthmatic patients to heat-stable antigens was different from that to the heat-labile antigens. exposure to antigens of h. influenzae is common in all the disease groups. skin test reactions ha ... | 1979 | 95481 |
| fetal hemoglobin: in vivo stimulation by d-thyroxine in adult marmosets. | reactivation of fetal hemoglobin gene expressions was studied in new-world monkeys (marmosets). after a series of intraperitoneal injections of d-thyroxine, fetal hemoglobin production was increased in three experimental animals. control animals were unaffected by the injection of the solvent. because the percentage of cells containing fetal hemoglobin exceeded the total percentage of fetal hemoglobin, it was concluded that thyroid hormone influenced the synthesis of fetal hemoglobin rather than ... | 1979 | 95517 |
| alpha-amylase activity of human neonate and adult saliva. | | 1979 | 95527 |
| [morphological and histochemical examinations of the white rat testis after long-term administration of low doses of lindan (author's transl)]. | | 1979 | 95557 |
| rna content and volume of nerve cell bodies in human brain. ii. subiculum in aging normal patients. | | 1979 | 95559 |
| erythrocytes of the human neonate. | this review has focused on the fetal erythrocyte. fetal erythropoiesis, the production of a red cell with a shortened life-span, and the unique characteristics of this cell with respect to membrane structure and function, energy metabolism, and hemoglobin synthesis have been described. the hematologic manifestations of a variety of red cell abnormalities of structure, metabolism, and hemoglobin synthesis have been presented, as well as a discussion of the anemia of prematurity and selected nutri ... | 1978 | 95579 |
| [use of the counterimmunoelectrophoretic method for typing the herpes simplex viruses]. | | 1979 | 95582 |
| oral progestational activity of spironolactone. | spironolactone has progestational activity in the rabbit and rhesus monkey. it produced glandular development in the endometrium of the estrogen-primed immature female rabbit over the dosage range of 50 to 200 mg/kg and, at 400 mg/monkey, it delayed estrogen-withdrawal bleeding in ovariectomized monkeys and it inhibited menstruation in regularly cycling monkeys. these data are consistent with the menstrual irregularity and amenorrhea seen in women during treatment with spironolactone. | 1978 | 95623 |