| lysodektose--a new trisaccharide from micrococcus lysodeikticus which is transformed into an aminoxyl free radical with the loss of an electron. | a new trisaccharide 6-0-(2-deoxy-2-(n-methyl)-hydroxylamino-beta-d- glucopyranosyl)-alpha,alpha-trehalose named lysodektose has been isolated from micrococcus lysodeikticus. in oxygenated solutions or in the presence of k3 fe (cn)6 lysodektose is transformed into a long lived free radical. spin trapping data are presented and functions are suggested for the substance. | 1991 | 1648021 |
| trypanosoma cruzi but not trypanosoma brucei fails to induce a chemiluminescent signal in a macrophage hybridoma cell line. | macrophage-trypanosoma cruzi interactions were studied by using a newly generated macrophage hybridoma cell line (2c11-12) that was selected for its capacity to produce high levels of reactive oxygen intermediates. this cell line was found to be a suitable host cell for t. cruzi, and intracellular parasitic development could be inhibited by activation with gamma interferon. when exposed to opsonized trypanosoma brucei, micrococcus lysodeikticus, or legionella pneumophila, the activated macrophag ... | 1991 | 1652563 |
| the inhibition of supercoiling activity of dna gyrase from micrococcus luteus caused by rufloxacin (mf 934) and mf 961. | | 1991 | 1653205 |
| mechanisms of clinical resistance to fluoroquinolones in enterococcus faecalis. | about 10% of 100 clinical isolates of enterococcus faecalis were resistant to greater than or equal to 25 micrograms of norfloxacin, ofloxacin, ciprofloxacin, and temafloxacin per ml. in this study, the dna gyrase of e. faecalis was purified from a fluoroquinolone-susceptible strain (atcc 19433) and two resistant isolates, ms16968 and ms16996. strains ms16968 and ms16996 were 64- to 128-fold and 16- to 32-fold less susceptible, respectively, to fluoroquinolones than was atcc 19433; mics of nonqu ... | 1991 | 1656852 |
| comparison of the effects of the new azalide antibiotic, azithromycin, and erythromycin estolate on rat liver cytochrome p-450. | erythromycin and some other macrolide antibiotics can first induce a cytochrome p-450 isozyme similar to the one induced in rats by pregnenolone-16 alpha-carbonitrile and then inhibit it by forming a stable cytochrome p-450-metabolite complex. the purpose of this study was to compare azithromycin, a novel 15-membered ring azalide, and erythromycin estolate for the potential to cause hepatic microsomal enzyme induction and inhibition in sprague-dawley rats. the daily oral administration of 800 mg ... | 1991 | 1656856 |
| detection of base damage in dna in human blood exposed to ionizing radiation at biologically relevant doses. | the alkaline elution technique for the detection of dna damage has been adapted to allow application on unlabelled blood cells. both the induction and subsequent repair have been studied of two classes of dna damage, viz, single-strand breaks and base damage recognized by the gamma-endonuclease activity in a cell-free extract of micrococcus luteus bacteria. the high sensitivity of the assay permitted the measurement of induction and repair of base damage after in vitro exposure of full blood und ... | 1991 | 1672355 |
| cloning, sequencing, and expression in escherichia coli of a streptococcus faecalis autolysin. | a streptococcus faecalis genomic bank was obtained by partial digestion with mboi and cloning into the sali restriction site of ptz18r. screening of about 60,000 escherichia coli transformants for cell wall lysis activity was done by exposing recombinant colonies grown on medium containing lyophilized micrococcus lysodeikticus cells to chloroform and toluene vapors in order to release proteins. because this procedure provoked cell death, colonies could not be used directly for transformant recov ... | 1991 | 1679432 |
| computerized image analysis of full-hand touch plates: a method for quantification of surface bacteria on hands and the effect of antimicrobial agents. | a method is described for quantification of the bacterial flora on the hand surface. computer-assisted image analysis of bacterial growth of large full-hand touch plates provides a quantifiable measure of the bacterial flora on the hand surface. image analysis pixel intensity values showed a significant correlation (p less than 0.0001) with colony forming unit values determined by the glove juice method. image analysis of impressions from hands treated with various antimicrobial agents in deterg ... | 1991 | 1679442 |
| low concentrations of acridine dimers inhibit micrococcus ap endonuclease through interaction with apurinic sites in dna. | the effect of dimeric dna intercalating compounds was assayed on a purified ap endonuclease from microccoccus luteus using apurinic supercoiled pm2 dna as a substrate. binding on apurinic sites was estimated through the competition with the intercalating compound, 9-nh2-ellipticine, which displays great specificity for apurinic sites. an acridine dimer with a spermine linker is at 0.1 microm the best inhibitor of cleavage at the apurinic site induced either by the ap endonuclease or by 9-nh2-ell ... | 1990 | 1690088 |
| camptothecin-stabilized topoisomerase i-dna adducts cause premature termination of transcription. | the antitumor agent camptothecin stabilizes type i topoisomerase-dna complexes. one of the primary cellular responses to camptothecin exposure is rapid cessation of rna synthesis. results obtained by using an in vitro transcription system supplemented with eukaryotic topoisomerase i show that this inhibition can be attributed to physical blockage of the rna polymerase by camptothecin-stabilized topoisomerase i-dna complexes on the dna template. the site of premature termination is located 10 bas ... | 1990 | 1696837 |
| dna synthesis in isolated resting nuclei: evidence for protease-dependent nonreplicative nucleotide incorporation. | we have used an in vitro assay to study the induction of dna synthesis by cytoplasmic extracts from the actively growing cell line molt 4 in nuclei isolated from quiescent human lymphocytes. the ttp incorporation which takes place in these nuclei has been shown to be inhibitable by serine protease inhibitors, particularly aprotinin. this dna synthesis has also been proposed to reflect the initiation of true dna replication; however, we find evidence that much, if not most, of this incorporation ... | 1990 | 1696899 |
| z-dna affinity chromatography. | in this chapter we have detailed a method that can be generalized to link virtually any dna substrate to a chromatography matrix at its ends via an avidin-biotin linkage. we have used this technique to construct a left-handed z-dna column for the purpose of identification and purification of z-dna-binding proteins. this technique for the linkage of dna to a column matrix by avidin-biotin technology can be modified, however, to produce linked multimeric sequences specific for regulatory or other ... | 1990 | 1697020 |
| selective recognition of dna antigenic determinants by murine monoclonal anti-dna antibodies. | to assess the immune recognition of dna in systemic lupus erythematosus, the antigenic specificity of monoclonal anti-dna antibodies from autoimmune mrl-lpr/lpr mice was investigated determinant specificity was assessed by elisa in terms of binding to a panel of ssdna antigens including calf thymus, human placenta, escherichia coli, clostridium perfringens, micrococcus lysodeikticus, salmon testes, chicken blood and murine dna. among the monoclonal antibodies, a variety of binding patterns was o ... | 1990 | 1698581 |
| enzymatic recognition of dna modifications induced by singlet oxygen and photosensitizers. | dna modifications induced either by photosensitization (illumination in the presence of methylene blue) or by chemically generated singlet oxygen (thermal decomposition of an 1,4-etheno-2,3-benzodioxin) are recognized and incised by repair endonucleases present in crude bacterial cell extracts. only a small fraction of the incised modifications are sites of base loss (ap-sites) sensitive to exonuclease iii, endonuclease iv from e. coli or to the uv-endonuclease from m. luteus. cell extracts from ... | 1990 | 1700366 |
| [stability of escherichia coli to the membranotropic antibiotic gramicidin s]. | the work was aimed at studying the effect of gramicidin s on the intact cells, spheroplasts and membrane specimens of escherichia coli k12s with the natural resistance to this antibiotic. the resistance was shown to be caused by the barrier properties of the cell wall: the spheroplasts were highly sensitive to the lytic action of gramicidin s. the differences in the sensitivity to gramicidin s of substrate oxidation carried by the membranes of e. coli and micrococcus luteus, a sensitive organism ... | 1990 | 1702180 |
| nucleotide sequences of nine trna genes from micrococcus luteus. | | 1990 | 1702204 |
| nucleotide sequences of two trna gene clusters from micrococcus luteus. | | 1990 | 1702205 |
| [antimicrobial and hemolytic activities of gradex]. | gradex is a polymer preparation resulting from formation of covalent bonds between the molecules of gramicidin s, a polypeptide antibiotic, and dextran, a polymeric carrier. antimicrobial and hemolytic activities of gradex were studied. it was shown that the antimicrobial activity of gradex was due to the presence of gramicidin s in its composition. the activity level was lower than that of gramicidin s. it was also found that the gradex reduced form in concentrations up to 300 micrograms/ml had ... | 1990 | 1702285 |
| right-sided infective endocarditis with acquired tricuspid valve stenosis associated with transvenous pacemaker: a case report. | five years prior to presentation, a 29-year-old woman received a transvenous pacemaker (ddd) for sick sinus syndrome and nodo-hisian pathology. after pacemaker insertion, she complained of recurrent febrile episodes. her pacemaker related endocarditis was quite unusual for the infecting organism (a micrococcus) and for an acquired tricuspid valve stenosis. the suspected cause was confirmed at surgery. | 1991 | 1715545 |
| novel anticodon composition of transfer rnas in micrococcus luteus, a bacterium with a high genomic g + c content. correlation with codon usage. | the number and relative amount of isoacceptor trnas for each amino acid in micrococcus luteus, a gram-positive bacterium with high genomic g + c content, have been determined by sequencing their anticodon loop and its adjacent regions and by selective labelling of trnas. thirty-one trna species with 29 different anticodon sequences have been detected. all the trnas have g or c at the anticodon first position except for trna(icgarg) and trna(ngaser), in response to the abundant usage of nnc and n ... | 1991 | 1717697 |
| clinical isolates of yeast produce a gliotoxin-like substance. | candida infections are major causes of morbidity in compromised human hosts, but our understanding of the virulence of candida remains incomplete. the possibility that toxic fungal metabolites belonging to the chemical class epipolythiodioxopiperzine (etp), which are reported to possess immunomodulating and antiphagocytic properties may be produced by candida species was investigated. reversed phase hplc analysis of flash evaporated chloroform extracts of 7 day cultures of clinical candida isola ... | 1991 | 1724551 |
| quantifying heterogeneity: flow cytometry of bacterial cultures. | flow cytometry is a technique which permits the characterisation of individual cells in populations, in terms of distributions in their properties such as dna content, protein content, viability, enzyme activities and so on. we review the technique, and some of its recent applications to microbiological problems. it is concluded that cellular heterogeneity, in both batch and continuous axenic cultures, is far greater than is normally assumed. this has important implications for the quantitative ... | 1991 | 1725477 |
| the metabolism of anthracene and 9,10-dimethylanthracene by bacteria isolated from waters. | the metabolism of two polycyclic aromatic hydrocarbons i.e. anthracene and 9,10-dimethylanthracene by micrococcus sp., pseudomonas sp. and bacillus macerans was examined. the above compounds were used as a sole carbon source for their growth. using the reversed-phase thin layer chromatography techniques a number of anthracene and 9,10-dimethylanthracene metabolites were isolated and their structures identified spectroscopically. these included anthracene and 9,10-dimethylanthracene cis-dihydrodi ... | 1991 | 1726622 |
| patterns of heavy and light chain utilization in the antibody response to single-stranded bacterial dna in normal human subjects and patients with systemic lupus erythematosus. | although anti-dna antibodies are generally considered to be specific markers for systemic lupus erythematosus (sle), antibodies binding dna from certain bacterial species can be found in the sera of normal subjects. to characterize the immunochemical properties of these antibodies, the igg subclass and light chain profile of antibodies to single-stranded micrococcal dna (mc dna) in the sera of normal subjects and patients with sle was determined. the anti-mc dna response in normal sera was predo ... | 1992 | 1728977 |
| bactericidal activity of c9-deficient human serum. | escherichia coli b/sm, strain 1-1, was killed dose dependently by human hereditary c9-deficient serum (c9dhs), which was shown to contain no c9 ag by an elisa method. on the other hand, human hereditary c7-deficient serum did not kill the bacteria under similar conditions. the bactericidal activity of c9dhs was inhibited by rabbit anti-c5 antibody but not by murine anti-c9 mab. the anti-c9 antibody decreased the bactericidal activity of normal human serum (nhs) to the level of that with c9dhs. s ... | 1992 | 1730876 |
| specific detection of monocytic lysozyme within normal and leukemic cells. | a murine monoclonal antibody against human lysozyme (ahl moab) was produced and tested on normal and leukemic monocytes using flow cytometry. the antibody gave a positive reactivity on normal monocytes permeabilized by saponin (82% to 98% of positive cells) and a negative reactivity on normal permeabilized neutrophils. this monocyte-specific reactivity had not been observed using a polyclonal antibody. nevertheless, immunoblotting detected lysozyme in both monocyte and polymorphonuclear leukocyt ... | 1992 | 1732014 |
| the major carotenoid pigment of a psychrotrophic micrococcus roseus strain: purification, structure, and interaction with synthetic membranes. | the major carotenoid pigment of a psychrotrophic micrococcus roseus strain was purified to homogeneity from methanol extracts of dried cells by reverse-phase liquid chromatography and was designated p-3. on the basis of the uv-visible, infrared, mass, and 1h nuclear magnetic resonance spectra of p-3, it was identified as bisdehydro-beta-carotene-2-carboxylic acid. the pigment interacted with synthetic membranes of phosphatidylcholine and dimyristoyl phosphatidylcholine and stabilized the membran ... | 1991 | 1744046 |
| purification of solanesyl-diphosphate synthase from micrococcus luteus. a new class of prenyltransferase. | the activity of solanesyl-diphosphate synthase from micrococcus luteus is stimulated by a high molecular mass fraction (hmf) which is separated from cell-free extracts of the same bacterium by deae-toyopearl chromatography followed by sephadex g-100 chromatography. by employing hmf in the assay procedure, solanesyl-diphosphate synthase was able to be purified to homogeneity and was found to be a homodimer with a monomeric molecular mass of 34 kda. in contrast to hexaprenyl- and heptaprenyl-dipho ... | 1991 | 1748647 |
| presence of a gene in the archaebacterium methanococcus vannielii homologous to secy of eubacteria. | the nucleotide sequence of a gene located at the promoter-distal side of the 'spectinomycin-operon' homologue of the archaebacterium methanococcus vannielii was determined. its derived amino acid sequence displayed 20% (identical positions) or 52% (including conservative exchanges) similarity, respectively, to secy from e coli. an alignment of the methanococcus secy with eubacterial secy sequences showed the existence of 10 membrane-associated primary structure domains in equivalent positions. t ... | 1991 | 1764515 |
| induction of immunity by latex beads and by hemolymph transfer in galleria mellonella. | injection of sterile latex beads into the hemocoel of last instar larvae of galleria mellonella provoked a strong defense reaction. cellular defense by hemocytes was followed by enhanced antibacterial activity in hemolymph. latex-injected insects showed increased survival rates after a challenge injection with high doses of bacteria. factors which stimulate the production of antibacterial activity could be demonstrated soon after injection by transfer of hemolymph from preinjected to untreated l ... | 1991 | 1773849 |
| synthesis of 3-furylmethylpenicillin using an enzymatic procedure. | 3-furylmethylpenicillin was synthesized in vitro from 3-furylacetic acid, 6-aminopenicillanic acid (6-apa), coa, atp and mg2+. the reaction was catalyzed in two steps by the enzymes phenyl-acetyl-coa ligase (pcl) from pseudomonas putida and acyl-coa: 6-apa acyltransferase (at) from penicillium chrysogenum. pcl catalyzes the activation of 3-furylacetic acid to 3-furylacetyl-coa (3-f-coa) and at acylates the amino group of 6-apa with the 3-furylacetyl moiety of 3-f-coa, releasing coa and 3-furylme ... | 1991 | 1778415 |
| structure and function of dnaa and the dnaa-box in eubacteria: evolutionary relationships of bacterial replication origins. | dnaa protein (a trans-acting element) and its binding sequence, dnaa-box: (a cis-acting element) are two elements essential for the initiation of chromosomal replication in escherichia coli and other enteric bacteria. recently these two elements have been found to be conserved in three gram-positive bacteria (bacillus subtilis, micrococcus luteus and mycoplasma capricolum) as well as in gram-negative pseudomonads. dnaa protein was also found to be essential in the initiation of the replication o ... | 1991 | 1779750 |
| preparation and properties of a lysozyme derivative in which two domains are cross-linked intramolecularly between trp62 and asp101. | a lysozyme derivative in which two domains were cross-linked intramolecularly was newly prepared by means of a two-step reaction. first, the beta-carboxyl group of asp101 in lysozyme was selectively modified with 2-(2-pyridyldithio)ethylamine in the presence of 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide hydrochloride. after reduction of the pyridyldithio moiety of asp101 modified lysozyme at ph 4.5 with dithiothreitol, the derivative was allowed to cross-link intramolecularly by reaction wi ... | 1991 | 1783601 |
| formation of an antibacterial metabolite from a new macrolide compound 23-o-benzyl-5-mycaminosyl-tylonolide (tmc-101), by a hepatic microsomal drug-metabolizing enzyme system. | upon incubation of 23-o-benzyl-5-mycaminosyl-tylonolide (tmc-101) with liver microsomes in the presence of an nicotinamide adenine dinucleotide phosphate-generating system, at least four metabolites were formed: two of them were also formed by an enzyme(s) in rat serum. one of the metabolites formed by liver microsomes possessed antibacterial activity comparable to tmc-101 as examined by bioautography using micrococcus luteus atcc 9341 as a tester strain. incubation of tmc-101 with rat serum deg ... | 1991 | 1802985 |
| purification of equine neutrophil lysozyme and its antibacterial activity against gram-positive and gram-negative bacteria. | lysozyme from equine neutrophil granulocytes was isolated in a pure form by fast performance liquid chromatography, i.e. ion-exchange chromatography and reversed-phase chromatography. the lysozyme lysed micrococcus luteus, bacillus subtilis and staphylococcus lentus and was also bactericidal against the gram-negative bacteria escherichia coli, klebsiella pneumoniae, bordetella bronchiseptica, and serratia marcescens. staphylococcus aureus and staphylococcus epidermidis were not lysed. the lysozy ... | 1991 | 1803722 |
| comparison of three commercial rapid agglutination test kits for identification of coagulase positive staphylococci from foods and animals. | three rapid agglutination assays for the identification of staphylococcus aureus monostaph (bionor a/s, skien, norway), staphyslide-test (biomerieux, lyon, france) and staph-rapid-test (roche, basel, switzerland), were compared. a total of 104 gram-positive, catalase positive cocci were tested: nineteen staphylococcus reference strains comprising 15 spp. (4 strains were coagulase positive), and 7 micrococcus reference strains comprising 4 spp.; 22 food isolates comprising 13 s. aureus, 8 coagula ... | 1991 | 1803928 |
| endothelin-1 inhibits pre-stimulated tracheal submucosal gland secretion and epithelial albumin transport. | 1. endothelin-1 potently contracts smooth muscle, including that in the airways. however, its effect on airway mucosal function has not so far been studied. 2. we have used the ferret whole trachea in vitro to examine the effect of endothelin-1 on tracheal smooth muscle tone, transepithelial potential difference (p.d.), submucosal gland secretion (including lysozyme secretion from serous cells) and active epithelial albumin transport. in addition we have examined the effects of endothelin on sub ... | 1991 | 1810592 |
| [antibiotic activity of marine gram-negative bacteria]. | an antimicrobial substance was isolated from the culture fluid of vibrio fischeri belonging to ++gram-negative sea bacteria. the substance was shown to be highly active against ++gram-positive cocci and less active against some other ++gram-positive bacteria and fungi. the active substance was defined as a low molecular weight compound which appeared to be thermostable and had positive ninhydrin reaction. the antibiotic formed when the culture was grown in the media containing sea water and prot ... | 1991 | 1814266 |
| phagocytosis of synthetic particles in earthworms: absence of oxidative burst and possible role of lytic enzymes. | the mechanisms of phagocytized materials (synthetic hema particles, micrococci) by earthworm coelomocytes were examined. despite the fact that coelomocytes have a high phagocytic activity, the chemiluminescent assay did not reveal increased production of oxygen radicals. ultrastructural changes of engulfed particles observed by electron microscopy may be caused by intracellular lytic enzymes. | 1991 | 1822449 |
| identification of staphylococcus and micrococcus species with the staphytest system. | a collection of 216 well-characterized strains of staphylococcus, micrococcus and stomatococcus was examined by a commercially available staphytest system (lachema, brno, czechoslovakia). the results of staphytest agreed with those of conventional tests. the staphytest permitted a clear-cut separation of staphylococcus from micrococcus and stomatococcus strains and correctly identified 104 of 145 (72%) staphylococcus strains after 24 h of incubation. however, it allowed the identification only o ... | 1991 | 1822843 |
| structural dynamics in f1atpase during the first reaction cycle of atp hydrolysis. | the velocity of atp hydrolysis, catalyzed by purified f1atpase from micrococcus luteus, was decelerated on decreasing the temperature. at 13 degrees c one reaction cycle is completed after 20 s. hydrolysis was triggered upon rapid mixing of the enzyme with atp. during the first reaction cycle, succeeding structural alterations of the f1atpase were traced by time resolved x-ray scattering. the scattering spectra obtained from consecutive intervals of 1 s, revealed the f1atpase to pass a conformat ... | 1991 | 1826273 |
| [identification of residual antibiotics in the tissues of slaughter animals using electrophoresis bioautography]. | an electrophoretic identification chart of the antibiotics penicillin, streptomycin, neomycin, erythromycin, tylosin and tetracyclines was made. minimum inhibiting concentrations of the above antibiotics were determined for the bacterial tester-strains bacillus subtilis bga, micrococcus luteus atcc 9341 and bacillus stearothermophillus v. calidolactis c 953. the obtained results were applied to identify residues of antibacterial substances in the tissues of slaughter animals, milk and other samp ... | 1991 | 1841480 |
| influence of repeated lyophilization on the survival of deinococcus proteolyticus, micrococcus luteus and escherichia coli. | repeated lyophilization of deinococcus proteolyticus, micrococcus luteus and escherichia coli cells results in a successive decrease of their survival. the survival curve is exponential with e. coli and m. luteus, and sigmoidal with a broad shoulder with d. proteolyticus both after repeated lyophilization and after uv- or gamma-irradiation. when cells were subjected to gamma-irradiation after a 20-fold freeze-drying, the corresponding survival curve became exponential without the shoulder. hence ... | 1991 | 1841852 |
| effect of the fungicides tridemorph and vinclozolin on soil microorganisms and nitrogen metabolism. | the effects of tridemorph and vinclozolin were studied on different types of microorganisms, urea hydrolysis and nitrification in soil and in culture. the fungicides adversely affected the population of bacteria, fungi and actinomycetes as a function of time of incubation. urea hydrolysis both in culture and soil were also inhibited by the fungicides, and tridemorph was more detrimental. in soil, 45 micrograms/g of tridemorph inhibited 50% of ammonification of urea, id50 for nitrite production w ... | 1991 | 1841872 |
| site-specific hypomethylation of c-myc protooncogene in liver nodules and inhibition of dna methylation by n-nitrosomorpholine. | the protooncogene c-myc was investigated in n-nitrosomorpholine-induced rat liver nodules to elucidate the role of altered dna methylation in chemical carcinogenesis. furthermore, micrococcus luteus dna and chicken erythrocyte dna were modified in vitro by reactive metabolites of n-nitrosomorpholine, generated by p450-dependent monooxygenases. the modified dnas were less methylated in vitro than control dnas by dna-(cytosine-5)-methyltransferase (dna methylase). the dna methylase assay and 32p-p ... | 1991 | 1859451 |
| [micrococcus luteus: a rare pathogen of valve prosthesis endocarditis]. | a rare case of prosthetic valve endocarditis caused by micrococcus luteus is described and compared with the few cases reported in the literature, as well as the clinical features, microbiological profile, therapy, and prognosis of common prosthetic valve endocarditis. micrococcus luteus is a constituent of the normal human buccal bacterial flora which forms yellowish colonies and appears as a gram-positive coccus typically arranged in tetrades. although of low virulence, the germ may become pat ... | 1991 | 1862670 |
| one-step purification of bacterial lipid macroamphiphiles by hydrophobic interaction chromatography. | bacterial lipid macroamphiphiles extracted with phenol/water can be purified in one step by hydrophobic interaction chromatography. lipids and the major part of protein are separated from macroamphiphiles during phenol/water extraction. coextracted nucleic acids, polysaccharides, and residual protein are effectively removed by column chromatography on octyl-sepharose whereby macroamphiphiles are primarily adsorbed and later eluted with a buffered propanol gradient. the procedure is applicable to ... | 1991 | 1862938 |
| characterization of staphylococcus hyicus with the atb 32 staph system and with conventional tests. | the atb 32 staph system correctly identified 45 of 54 staphylococcus hyicus cultures isolated from pigs and cattle. the biochemical profiles of the remaining nine cultures were not listed in the product data base. the 40 porcine and 14 bovine cultures resulted in three and seven different biochemical profiles, respectively. in parallel experiments, almost all s. hyicus cultures showed hemolytic reactions on chocolate agar, had camp-like reactivities in the zone of lysis of the staphylococcal bet ... | 1991 | 1864941 |
| [value of the technique of cellular lysis by thermic shock in the isolation of bacteria causing osteoarticular infections]. | the purpose of this work is to compare, a conventional technique, for bacterial isolation, with a lysis-centrifugation method using a rapid freezing in liquid nitrogen, followed by decongelation at 37 degrees c for bone and joint samples. the bone and joint specimens were biopsies and punctions (35 cases) or fistula (10 cases). the residual antibiotic activity of the sample was determined using a susceptible strain of micrococcus luteus and of staphylococcus epidermidis. among the 45 samples, 20 ... | 1991 | 1881691 |
| histones and dna methylation in mammalian chromatin. differential inhibition by histone h1. | histones (from calf thymus or from human placenta), if renatured in the presence of edta, caused a severe inhibition of in vitro methylation of double-stranded dna (from micrococcus luteus) by human placenta dna methyltransferase. the absence of edta during the histone renaturation procedure abolished--at least in the 'physiological' range of the histones/dna ratio--the inhibition. the h1 component was responsible for this inhibition, no effect being exerted by the other histones. h1 preparation ... | 1991 | 1883842 |
| [a comparative study of 2 substances produced by the streptococcus sp. thom-1606 strain]. | among the substances secreted by streptococcus sp. thom-1606 [correction of tom-1606], two substances exhibiting opposite biological action have been detected. their antigenic structure, as indicated by the data of immunoprecipitation in agar, are not identical. the compounds contained in the substance with antibacterial action have molecular weight below 10 kd, stokes' radium equal to 1.10 +/- 0.15 nm and electrophoretic mobility approximating that of d1-fraction of human blood serum. the compo ... | 1991 | 1887720 |
| inverse correlation in nutritionally variant streptococci between the production of bacteriolytic activity and sensitivity to a streptococcus pyogenes bacteriocinlike inhibitory substance. | nineteen strains of nutritionally variant streptococci (nvs) were tested for bacteriolytic activity and for their production of and sensitivity to streptococcal bacteriocinlike inhibitory substances (blis). none appeared to produce blis. an inverse relationship was found between the sensitivity to blis and the production of bacteriolytic activity against micrococcus luteus. all but one of the 14 streptococcus defectivus isolates were sensitive to the blis of s. pyogenes p5, and these isolates we ... | 1991 | 1890190 |
| [staphyogram, a new rapid identification kit for the aerobic, gram-positive, catalase-positive cocci--application of fluorometric microplate hybridization for the pre-identification of 386 isolates used]. | a new simplified test kit, staphyogram plate, was developed for 4-hr identification of aerobic, gram-positive and catalase-positive cocci. the plate has 18 wells, in which different dehydrated substrates and nutrients are fixed. an 18-hr agar-culture suspension of a test strain with a turbidity of mcfarland no. 4 was distributed into all wells in 50-microliters quantities. after 4-hr incubation at 37c, the profile number was obtained by summarizing positive reactions. the ability of the plate to ... | 1991 | 1890731 |
| periodic acid-schiff-positive organisms in primary cutaneous bacillus cereus infection. case report and an investigation of the periodic acid-schiff staining properties of bacteria. | primary cutaneous bacillus cereus infection frequently presents as a single necrotic bulla on the extremity of an immunocompromised patient. in lesional biopsy specimens and smears, the large gram-positive rods of b cereus may be mistaken for clostridium species. this is a potentially serious error, as bacillus species are resistant to penicillin and other beta-lactam antibiotics. we studied a case in which large periodic acid-schiff-staining organisms were seen in the biopsy specimen from a nec ... | 1991 | 1900984 |
| new triene-beta-lactone antibiotics, triedimycins a and b. | | 1991 | 1903377 |
| [production of hydroxy and oxo fatty acids by microorganisms as a model of adipocere formation]. | microbial synthesis of hydroxy and oxo fatty acids was studied as one of the model of experimental adipocere formation. conversion of various fatty acids into 10-hydroxy and 10-oxo fatty acids by micrococcus luteus was also studied. fatty acids possessing cis-9-unsaturated forms were converted into 10-hydroxy and 10-oxo fatty acids. on the other hand, enoic acids possessing trans-9-unsaturated form or the ones which do not have double bond at the 9 -carbon position were inactive as substrates. 1 ... | 1991 | 1905675 |
| the occurrence and growth of microorganisms during the fermentation of fish sausage. | minced fish (mullet) sausage mixes containing added sugar, salt, nitrate, nitrite and spices were fermented (48 h, 30 degrees c) by indigenous flora or by a starter culture (pediococcus acidilactici) and the microbial ecology and behaviour of various bacteria was monitored. pediococcus pentosaceus and lactobacillus plantarum dominated the indigenous fermentation, achieving populations of 10(7)-10(8) cfu/g by 48 h, and decreasing the ph of the mix to 4.5-4.7. significant growth (10(5)-10(7) cfu/g ... | 1991 | 1909546 |
| optically detected triplet-state magnetic resonance studies of the dna complexes of the bisquinoline analogue of echinomycin. | the polymeric dna and model duplex oligonucleotide complexes of the bisquinoline analogue of echinomycin (2qn) have been studied by optical detection of triplet-state magnetic resonance (odmr) spectroscopy, with the quinoline chromophores of the drug used as intrinsic probes. plots of odmr transition frequencies versus monitored wavelength revealed heterogeneity in the phosphorescence emission of 2qn which was ascribed to the presence of a major and minor conformation of the drug in aqueous solu ... | 1991 | 1911753 |
| cell separation system studied by mixed culture of single wild strain with tetrads-forming mutant strain of micrococcus luteus. | mixed culture study of singly occurring wild strain ifo 3333 of micrococcus luteus and a tetrads-forming mutant strain mt, in the absence or presence of trypsin, supported our previous assumption that at least two kinds of separation systems were involved in cell separation of m. luteus, the one having a physiological role in cutting off the outermost layer of the cell wall (separation system-om) and the other in cutting off the inner layer of the "proper" cell wall or the septum (separation sys ... | 1991 | 1934012 |
| antibacterial activity of two chalcones, xanthoangelol and 4-hydroxyderricin, isolated from the root of angelica keiskei koidzumi. | two chalcones, xanthoangelol (i) and 4-hydroxyderricin (ii), isolated from the root of angelica keiskei koidzumi (umbelliferae) showed antibacterial activity against gram-positive pathogenic bacteria. the activity of i on micrococcus luteus ifo-12708 (minimum inhibitory concentration (mic), 0.76 microgram/ml) was the same potency as that of gentamicin, which is used as a standard. although the activity of both chalcones on plant-pathogenic bacteria was lower than that of streptomycin sulfate, us ... | 1991 | 1934181 |
| palladium, platinum, cadmium, and mercury complexes with neutral isoorotic and 2-thioisoorotic acids: ir and nmr spectroscopies, thermal behavior and biological properties. | seven complexes containing neutral isoorotic and 2-thioisoorotic acids, as well as thiocyanate and chloride anions as lignands, have been synthesized and characterized by means of both spectral (ir, 1h, and 13c nmr) and thermal (tg and dsc) methods, as well as conductivity measurements. spectral data suggest that any binding metal-ligand mode for uracil derivatives is not easy to propose. therefore, isoorotic ligands must link through some oxygen atom. likewise, 2-thioisoorotic acid seems to be ... | 1991 | 1940899 |
| airborne microorganism monitoring: a comparison of several methods, including a new direct counting technique. | samples of aerosol from the surrounding air were collected by forcing them to impact onto a solid nutrient medium, onto membrane filters, or onto microscope slides on microcover slips. the samples were cultivated or viewed in a fluorescence microscope by using a technique developed by us, or investigated by scanning electron microscopy. the amounts of microorganisms found by cultivation method, i.e. those forming microcolonies (cfu), were on average 85% lower than the amounts determined by the f ... | 1991 | 1950200 |
| comparative studies on bacteriolytic properties of staphylococcus chromogenes and staphylococcus hyicus. | staphylococcus chromogenes and staphylococcus hyicus showed bacteriolytic activities towards a micrococcus luteus reference strain. this was demonstrated on tryptone soya agar containing m. luteus cells. both bacteriolytic enzymes could be isolated by ionic exchange chromatography and subsequent gel filtration. the isolated bacteriolysin of s. chromogenes lysed the m. luteus reference culture, was heat inactivated by 95 degrees c and precipitated specifically with antiserum produced against the ... | 1991 | 1950253 |
| [the antilysozyme activity of meningococci]. | the antilysozyme activity of 184 meningococcal strains was studied. such activity was found in all strains within the range 1-25 micrograms/ml, which was due to the heterogeneity of bacterial population. strains varying in the total level of their lysozyme activity differed in the population structure by this sign. preparations inhibiting antilysozyme activity could be differentiated according to the character of their action by the method of the population analysis. in the process of phagocytos ... | 1991 | 1950256 |
| airborne dust, ammonia, microorganisms, and antigens in pig confinement houses and the respiratory health of exposed farm workers. | this study investigated the environmental conditions on pig farms and the respiratory health of pig farmers and their immunological response to airborne contaminants. airborne concentrations of dust and ammonia were measured in 20 pig houses; viable microorganisms, endotoxins, and aeroallergens were measured in 6 of these houses, chosen to represent the range in dustiness. the 29 farmers employed on the farms completed a questionnaire and underwent lung function tests; 24 of them provided blood ... | 1991 | 1951065 |
| in vitro enzymatic synthesis of new penicillins containing keto acids as side chains. | seven different penicillins containing alpha-ketobutyric, beta-ketobutyric, gamma-ketovaleric, alpha-ketohexanoic, delta-ketohexanoic, epsilon-ketoheptanoic, and alpha-ketooctanoic acids as side chains have been synthesized in vitro by incubating the enzymes phenylacetyl coenzyme a (coa) ligase from pseudomonas putida and acyl-coa:6-aminopenicillanic acid acyltransferase from penicillium chrysogenum with coa, atp, mg(2+), dithiothreitol, 6-aminopenicillanic acid, and the corresponding side chain ... | 1991 | 1952871 |
| [detection of aquatic microorganisms from the black sea--producers of restriction endonucleases]. | 300 clones of microorganisms isolated at different stations and from different depths in the black sea were screened for restriction endonucleases production. the production of restriction endonucleases was found in 17 clones screened. three of them were identified to be alteromonas haloplanktis b1. restriction endonuclease ahab1 is an isoshizomer of sau961. an identified alteromonas haloplanktis clone b8 produces ahab8i restriction endonuclease the prototype to which is kpni. of the clones isol ... | 1990 | 1964718 |
| hygienic hand disinfection: a comparative study with chlorhexidine detergents and soap. | the efficacy of two chlorhexidine hand-wash detergents and liquid soap was compared in a laboratory trial using artificial contamination of fingers with micrococcus and serratia. agents were assessed for both a rapid and sustained effect after a single contact, and for a cumulative persistent effect after multiple contact over four days. disinfectant activities were compared by statistical analysis of log reduction factors and log count time gradients (decimal reduction times). the latter analys ... | 1990 | 1972948 |
| micrococcus and stomatococcus spp. from human infections. | infections with micrococcus spp. in six patients and stomatococcus mucilaginosus in one patient are described. two of the micrococcus infections occurred in leukaemic patients with indwelling lines, six episodes occurred in three patients undergoing continuous ambulatory peritoneal dialysis and one occurred in a patient with a ventriculo-peritoneal shunt. stomatococcus was isolated from fluid draining from a sub-dural haematoma. colony morphology, oxidase reaction and resistance to nitrofuration ... | 1990 | 1974908 |
| inhibition of bactericidal and bacteriolytic activities of poly-d-lysine and lysozyme by chitotriose and ferric iron. | in a previous report from this laboratory (n. j. laible and g. r. germaine, infect. immun. 48:720-728, 1985), evidence was presented to suggest that the bactericidal actions of both reduced (i.e., muramidase-inactive) human placental lysozyme and the synthetic cationic homopolymer poly-d-lysine involved the activation of a bacterial endogenous activity that was inhibitable by n,n',n"-triacetylchitotriose (chitotriose). in the present investigation however, we found that the bactericidal and bact ... | 1991 | 1987082 |
| identification and characterization of rna polymerase sigma factor from micrococcus luteus. | the promoters of micrococcus luteus, a bacterium whose chromosomal dna has a high g + c content (74%), diverge from the consensus prokaryotic promoter in having gc-rich dna sequences at less important positions (nakayama, m., fujita, n., ohama, t., osawa, s., and ishihama, a. (1989) mol. gen. genet. 218, 384-389). in order to compare the promoter selectivity of rna polymerase between m. luteus and escherichia coli, we purified the enzyme from both organisms. the sets of promoters recognized by t ... | 1991 | 1993665 |
| enhancement of photorepair of ultraviolet-damage by preillumination with fluorescent light in cultured fish cells. | fluorescent light (fl) illumination of rbcf-1 cells, derived from a goldfish, prior to 254 nm uv-irradiation enhanced their ability to photorepair. the cells were illuminated with fl for 1 h (29 w/m2) and incubated for 8 h in the dark before being irradiated with 10 j/m2 uv. the surviving fraction of fl-treated cells after uv-irradiation rose about 7-fold (from 3 to 20%) by 20 min photorepair treatment with the same fl source, whereas 4-fold (from 1.6 to 6%) in the fl non-treated cells. flow cyt ... | 1991 | 2011625 |
| importance of the o6 position of guanine residues in the binding of dna methylase to dna. | methylation of micrococcus lysodeikticus dna by purified dna methylase isolated from l1210 leukaemia cells is potently and specifically inhibited by both hetero and homoribo and deoxyribopolynucleotides containing guanine residues. the inhibitory effect is unaffected by chain length, but is abolished when the o6 residue of guanine is substituted as in poly[d(o6meg)]20. potent inhibition is also shown by polyinosinic and polyxanthylic acids, but not by polyadenylic acid or by heteropolymers conta ... | 1991 | 2015298 |
| an alternative strategy for infection control of anesthesia breathing circuits: a laboratory assessment of the pall hme filter. | contaminated breathing systems have been responsible for nosocomial upper respiratory tract and pulmonary infections in patients undergoing general anesthesia. the current infection control guidelines for anesthesia breathing circuits require single-patient use or high-level disinfection of breathing tubes, y-connector, and reservoir bag. an alternative infection control strategy has been suggested that incorporates placement of a microbial filter downstream from the y-connector between the circ ... | 1991 | 2018223 |
| lysozyme concentrations in the tears of cattle, goats, and sheep. | tear samples were collected from 1 eye of each of 40 cows, 27 sheep, 5 goats, and 5 human beings. additionally, 10 bovine tear samples were pooled and concentrated. spectrophotometric assays, using micrococcus lysodeikticus, were performed on each sample to detect lysozyme activity expressed in hen egg lysozyme (hel) equivalents. lysozyme activity was not detected in tears of cows, but 158.8 +/- 159.3 mg of hel/ml was detected in tears of sheep, 220.7 +/- 37.5 mg of hel/ml in tears of goats, and ... | 1991 | 2021261 |
| enzymatic methylation of cytosine in dna is prevented by adjacent o6-methylguanine residues. | the effect of o6-alkylation of guanine residues on the enzymic methylation of cytosine has been studied using synthetic oligonucleotides in which all guanines in cytosine-guanine sequences at potentially methylatable sites are replaced by o6-methylguanine. in contrast with the unmodified forms, which showed high acceptance activity for methyl-3h-labeled groups from s-adenosyl-l-[methyl-3h]methionine in the presence of dna methylase, the modified oligonucleotides were not substrates for the enzym ... | 1991 | 2022628 |
| applications of gas chromatography-mass spectrometry in the study of androgen and odorous 16-androstene metabolism by human axillary bacteria. | the known involvement of axillary microflora with under-arm odour (uao) production led us to determine whether the odorous 16-androstene steroids are formed in the axilla by bacterial metabolism of an odourless precursor such as testosterone. axillary bacteria from 34 men were selectively cultured for aerobic coryneform bacteria (acb), micrococcaceae and propionibacteria. overnight suspensions of bacteria were incubated separately at 37 degrees c for two weeks with radiolabelled testosterone plu ... | 1991 | 2026727 |
| nucleoprotein hybridization: a method for isolating active and inactive genes as chromatin. | the developmentally regulated sea urchin early histone gene repeat (suehgr) from strongylocentrotus purpuratus was isolated as chromatin by nucleoprotein hybridization. this technique is a novel method to isolate specific sequences as chromatin. because the purification scheme is based only on the gene sequence and is independent of other physical properties such as protein composition and transcriptional activity, we were able to isolate the same gene in different functional states. gene size c ... | 1991 | 2030947 |
| viable but nonculturable bacteria in drinking water. | klebsiella pneumoniae, enterobacter aerogenes, agrobacterium tumefaciens, streptococcus faecalis, micrococcus flavus, bacillus subtilis, and pseudomonas strains l2 and 719 were tested for the ability to grow and maintain viability in drinking water. microcosms were employed in the study to monitor growth and survival by plate counts, acridine orange direct counts (aodc), and direct viable counts (dvc). plate counts dropped below the detection limit within 7 days for all strains except those of b ... | 1991 | 2039237 |
| occurrence of two structural types of mercury reductases among gram-positive bacteria. | structural variants of mercury reductase containing the n-terminal domain, which is easily cleaved by trypsin, have been found in gram-positive bacteria with a low genomic g + c content (bacillus, staphylococcus and, possibly, some other genera). mercury reductases without the n-terminal domain and relatively resistant to limited proteolysis are typical for gram-positive bacteria with a high genomic g + c content (arthrobacter, citreobacterium, micrococcus, mycobacterium, rhodococcus). both type ... | 1991 | 2040434 |
| protein synthesis in vitro by micrococcus luteus. | bacillus subtilis and related gram-positive bacteria which have low to moderate genomic g + c contents are unable to efficiently translate mrna derived from gram-negative bacteria, whereas escherichia coli and other gram-negative bacteria are able to translate mrna from both types of organisms. this phenomenon has been termed translational species specificity. ribosomes from the low-g + c-content group (low-g + c group) of gram-positive organisms (b. subtilis and relatives) lack an equivalent to ... | 1991 | 2045372 |
| the relationship of acanthamoeba to the external eye flora. | | 1991 | 2047672 |
| effects of high hydrostatic pressure on characteristics of pork slurries and inactivation of microorganisms associated with meat and meat products. | pork slurries inoculated with various test microorganisms were prepared and subjected to high hydrostatic pressure at 1000 to 6000 atm for 10 min at 25 degrees c to examine for the pressure effects on characteristics of the slurries and the inactivation of the microorganisms associated with meat and meat products. pressure treatment at higher than 3000 atm caused coagulation and discoloration of the pork slurries. harder and more white coagulants were obtained by increasing the pressure. pressur ... | 1991 | 2049285 |
| preferential excision repair and non-preferential photoreactivation of pyrimidine dimers in the c-ras sequence of cultured goldfish cells. | the time courses of excision repair and photoreactivation of pyrimidine dimers induced by 254-nm uv were examined in the genome overall and in the c-ras sequence of rbcf-1 cells derived from a goldfish, by the use of uv endonuclease of micrococcus luteus and alkaline agarose gel electrophoresis. excision repair was more efficient in the ras sequence than in the genome overall, whereas no differences in efficiency of photoreactivation were detected. these results suggest that excision repair is a ... | 1991 | 2052010 |
| cibacron blue 3g-a inhibits cell separation of gram-positive bacteria. | a triazine dye, cibacron blue 3g-a (cb), is an inhibitor of cell separation of staphylococcal spp. therefore, we examined the effect of cb on growth of gram-positive bacteria other than staphylococcus. cb added to the medium of growing cultures of strains of genus micrococcus, streptococcus, lactobacillus and bacillus caused inhibition of cell separation. moreover, in case of bacillus and lactobacillus, individual cells were elongated as filament. strains of the genus micrococcus were as sensiti ... | 1991 | 2059100 |
| interconversion of radical and nitrone forms of lysodektose--a new trisaccharide from micrococcus lysodeikticus. | isolated from micrococcus lysodeikticus, 6-o-(2-deoxy-2-(n-methyl)hydroxilamino-beta-d-glucopyranosyl)-alph a-alpha- trehalose (lysodektose) is oxidized by k3fe(cn)6 in a stepwise manner to become a nitroxyl radical and a nitrone with a double bond in the fragment o-n = ch2 which could be reduced to the original hydroxylamine form with sodium borohydride. thus derivatives of lysodektose specifically labelled with 2h and (or) 3h in the methyl group are easily obtained. when oxidized in cells pois ... | 1991 | 2059316 |
| lysozyme is an ozone-sensitive component of alveolar type ii cell lamellar bodies. | exposure of rats to 3 ppm ozone for up to 8 h results in significant changes in lamellar bodies, the surfactant storing organelles of type ii cells. we have previously shown that a 14 kda lamellar body protein is decreased as early as 4 h after the onset of ozone exposure. we have isolated this ozone-sensitive protein from rat lung lamellar bodies and identified it as lysozyme by immunochemical methods, as well as by its amino acid composition, n-terminal amino acid sequence and bacteriolytic ac ... | 1991 | 2065105 |
| changes in the spectrum of organisms causing bacteremia and fungemia in immunocompromised patients due to venous access devices. | a significant increase in the use of vascular access devices has changed the spectrum of organisms causing bacteremia and fungemia at memorial sloan-kettering cancer center. this paper documents the 1988 laboratory experience with bacteremia and fungemia and contrasts some of that data with information obtained in 1984. in 1988, 439 tunnelled-catheters and 355 ports were inserted in patients; 2,778 organisms were subsequently recovered from 933 episodes of bacteremia and fungemia. fifty-percent ... | 1990 | 2073897 |
| phallus-inflammation of ganders: clinical observations and comparative bacteriological examinations of healthy and altered organs. | the examination of acute or chronically altered phallus-tissues of ganders revealed microorganisms belonging to the genera mycoplasma, bacteroides, clostridium, streptococcus, micrococcus, staphylococcus, lactobacillus, corynebacterium, escherichia, campylobacter, proteus, pseudomonas and candida and to the family pasteurellaceae, isolated in different frequencies. bacteriologic examinations of the phallus-tissues and cloacal mucous membranes of healthy juvenile ganders showed microorganisms of ... | 1990 | 2082630 |
| [milk chloride level as an indicator of bovine mastitis]. | the aim of this study was to determine an influence of udder infection on milk chloride level and on milk productivity of cows of black and white race. bacteriological analysis was performed by bacterial isolation from milk collected in sterile conditions from single lobes of mammary gland. the study was aimed to detect the following bacteria: streptococcus agalactiae, streptococcus uberis, streptococcus agalactiae, staphylococcus aureus, staphylococcus epidermidis, actinomyces pyogenes, enterob ... | 1990 | 2084451 |
| assessment of a contact-plate sampling technique and subsequent quantitative bacterial studies in atopic dermatitis. | the aerobic bacterial flora of 20 patients with atopic dermatitis and 19 control subjects was extensively sampled using the williamson and kligman scrub technique and a contact-plate method (cysteine lactose electrolyte deficient media). comparison of the two quantitative techniques showed that the contact plate is a reliable and convenient alternative to the scrub technique for the quantification of staphylococcus aureus, micrococci and coagulase negative staphylococci. quantification of bacter ... | 1990 | 2095181 |
| paradoxical response of enterococcus faecalis to the bactericidal activity of penicillin is associated with reduced activity of one autolysin. | ten clinical isolates of enterococcus faecalis were examined for susceptibility to the bactericidal activity of penicillin. four of these had mbcs of penicillin equal to 2 to 4 x the mic, and six exhibited a paradoxical response to penicillin, i.e., the bactericidal activity of the antibiotic had a concentration optimum at 2 to 4 x the mic and decreased significantly at concentrations above this. we found that the paradoxical response to penicillin was an intrinsic and stable property of a strai ... | 1990 | 2109578 |
| the aerobic psychotrophic populations on meat and meat contact surfaces in a meat production system and on meat stored at chill temperatures. | at a city abattoir, a wholesaler and 10 different supermarkets, surface microbiological samples were taken of carcasses, hands and apron fronts of members of staff and equipment (mincers and saws). in addition, minced meat, packaged and displayed in chilled cabinets, was also sampled. carcasses, personnel surfaces and equipment were monitored by a modified agar sausage technique. from each of the highest dilution psychotrophic plate counts, five colonies were selected randomly, isolated and iden ... | 1990 | 2112534 |
| bacitracin-induced proteins in bacillus subtilis and bacillus thuringiensis and their relationship with resistance. | bacitracin induced one protein (bacitracin-induced protein [bip]) in bacillus thuringiensis and two proteins (bip1 and bip2) in bacillus subtilis that were localized in the membrane. divalent cations acted as cofactors for induction in all three cases. growth was initially inhibited by the antibiotic, but following induction of proteins growth resumed. b. subtilis cells possessing bips were able to duplicate at a normal rate in the presence of bacitracin. the amount of b. subtilis bips diminishe ... | 1990 | 2113795 |
| [survey of microbes in hospital environment]. | bacterial count and species distribution in hospital ward was investigated. it was shown that the in air total bacterial count was maximum in the second season of the year and the number of streptococcus above the standard was in the first season. bacteria in the air of the ward by number was in this order: micrococcus tetragenus, staphylococcus, bacillus subtilis, streptococcus and corynebacterium. according to the number of bacteria on the equipment and door handle of the ward, the order, was ... | 1990 | 2114272 |
| [fecal sigs and lysozyme excretion in breast feeding and formula feeding]. | the bioavailability of siga and lysozyme from human milk was investigated in a total of 41 infants by radial immunodiffusion and by the micrococcus lysodeicticus method, respectively. in four different pools of human milk used for balance studies the siga concentrations ranged between 2,200 and 17,850 mg/l. the lysozyme concentration varied from 64.5 to 283.5 mg/l. on human milk feeding the excretion of siga in 19 infants was 3,200 (0-8,200) mg per litre and 9.7 (0-131) mg lysozyme per litre, re ... | 1990 | 2115935 |
| [polyamines in representative taxa of coryneform and other bacteria]. | the composition of polyamines is studied for the first time in representatives of the genus micrococcus and taxon "conglomeratus", strains staphylococcus aureus ccm 209, deinococcus erythromyxa ccm 706 as well as of erwinia carotovora atcc 15713 polyamines, which are not extracted by perchloric acid. considerable amounts of spermine and rarely of spermidine are revealed in cells of gram positive microorganisms, that differs them from gram negative bacteria possessing high concentrations of putre ... | 1990 | 2115965 |
| production of poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acids. | alcaligenes latus, alcaligenes eutrophus, bacillus cereus, pseudomonas pseudoflava, pseudomonas cepacia, and micrococcus halodenitrificans were found to accumulate poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acid [p(hb-co-hv)] copolymer when supplied with glucose (or sucrose in the case of a. latus) and propionic acid under nitrogen-limited conditions. a fed-batch culture of a. eutrophus produced 24 g of poly-beta-hydroxybutyric acid (phb) liter-1 under ammonium limitation conditions. when ... | 1990 | 2117877 |
| biosynthetic elongation of isolated teichuronic acid polymers via glucosyl- and n-acetylmannosaminuronosyltransferases from solubilized cytoplasmic membrane fragments of micrococcus luteus. | cytoplasmic membrane fragments of micrococcus luteus catalyze in vitro biosynthesis of teichuronic acid from uridine diphosphate d-glucose (udp-glucose), uridine diphosphate n-acetyl-d-mannosaminuronic acid (udp-mannaca), and uridine diphosphate n-acetyl-d-glucosamine. membrane fragments solubilized with thesit (dodecyl alcohol polyoxyethylene ether) can utilize udp-glucose and udp-mannaca to effect elongation of teichuronic acid isolated from native cell walls. when udp-glucose is the only subs ... | 1990 | 2118507 |
| [the effect of dexamethoxin on the integrity of cytoplasmic membrane in gram-positive and gram-negative microorganisms]. | decamethoxin is shown to be able to increase membrane permeability of pseudomonas aeruginosa, escherichia coli and micrococcus lysodeikticus, that is confirmed by a loss of compounds with the absorption maximum at 260 nm by cells. parallel with this the number of viable individuals has fallen and activity of dehydrogenases has been inhibited. the aspartate and alanine aminotransferase activity was not inhibited by decamethoxin and even increased. decamethoxin lysed the protoplasts of the tested ... | 1990 | 2120553 |