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stability of mmpi profiles of female psychiatric inpatients. 20144379981
stability of mmpi profiles of female psychiatric inpatients. 20144379981
[combined treatment of onychomycoses with griseofulvin in combination with topical therapy]. 20144226921
[a study of the uroprecipitation reaction in psoriasis]. 20144247254
[leiomyoma of the second duodenum]. 20144299962
incidence of diabetic retinopathy and blindness: a population-based study in rochester, minnesota.among the 1135 rochester residents discovered to have diabetes in the period 1945-69, the prevalence of retinopathy was 2.6% at the time of initial diagnosis. among those free of retinopathy at diagnosis of diabetes, the subsequent incidence of any retinopathy was 17.4 per 1000 person-years and for proliferative retinopathy alone was 1.6 per 1000 person-years, based on 12,000 person-years of follow-up. the incidence rate of retinopathy was almost three times greater among residents with insulin- ...20144042796
conjoint treatment of spouses: clinical experiences. 20144020526
[some disorders of the cardiostimulating system from the clinical aspect (author's transl)]. 20144419047
energetics of multihelix interactions in protein folding: application to myoglobin. 20144027345
noise-induced hearing loss and portable radios with headphones.portable radio/cassette players with headphones have gained increasing popularity in recent years. volume settings are often increased to override environmental noise, perhaps placing the listener at risk for noise-induced hearing loss (nihl). a total of 190 public college students in nyc were studied via a self-administered questionnaire regarding the volume setting used and weekly exposure in hours to these units. three popular models were tested using a bruel and kjaer sound level meter, octa ...20144030232
[historical significance of the achievements of medicine and public health in world war ii 1941-1945]. 20143900561
the effects of acupuncture versus placebo in the treatment of headache.forty-eight patients were entered into a placebo (mock tns) versus acupuncture study to assess the effect of these therapies on headache. treatment was evaluated by the use of patient diaries; each patient completed a daily diary for 4 weeks prior to treatment during 6 weeks of therapy and for 24 weeks of follow-up. thirty-nine patients completed treatment and follow-up. at most acupuncture appears to be approximately 20% more effective than a placebo in alleviating headache but no statistically ...20143982837
[effect of different oculomotor regimens on the development of the functional potentials of the eye]. 20144047580
[alkaline phosphatase changes in leukocytes of workers with long-term exposure to some aromatic hydrocarbons]. 20154718091
radiation dose to humans from 75 se-l-selenomethionine. 20154622232
[principles of emergency aid in some forms of vestibular dysfunction]. 20154538748
[protective effect of sh compounds on the radiation-induced mitotic delay. 3. protection by cysteamine (author's transl)]. 20154533789
[fundamental study for diagnosis of pulmonary segment by scintillation camera. 2]. 20154508402
[deadyn: treatment of behavior disorders]. 20154384083
comparison of tests of hypothalamo-pituitary-adrenocortical function in pituitary disease. 20154319576
[nutrition of patients with skull-brain injuries]. 20154363417
atheroma of the aortic bifurcation. 20165069229
[sports and health in our environment]. 20165052281
the diagnosis of bilharzial bladder neck obstruction: evaluation of criteria in 140 cases. 20165010706
simultaneous separation of common mammalian 4 -3-oxosteroids and oestrogens using two-dimensional thin-layer chromatography. 20165063012
[treatment of carrier typhoid and paratyphoid carriage in the use of ampicillin]. 20164958990
[case of death due to paroxysmal hypoglycemia caused by hypertrophy of the langerhans islets and the use of diabetol]. 20164873512
relationship between verbal reinforcers, source and task difficulty for youthful felons and non-felons. 20164820506
[indications for surgery of solitary gallbladder stone (author's transl)]. 20164809958
urinary vaginal fistulae: a series of 152 patients treated in a small hospital in ghana. 20164807491
[the so-called physiologic lagophthalmos during sleep]. 20164789750
car radiator burns: a report on 72 cases.seventy-two cases of car radiator burns (crb) were treated in the burns unit, hamad medical corporation, doha, qatar, over a 6-year period (1982-87). all the patients were males and most were between 20 and 40 years old. chest wall, face and right upper limb were the commonest sites involved. most of the patients suffered from relatively minor scalds. the scenario of the accidents as well as the topography of the burned areas were characteristic to this particular type of injury. the exceptional ...20092765150
intra-ocular pressure changes during rapid sequence induction of anaesthesia. a comparison between thiopentone and suxamethonium and thiopentone and atracurium.the changes in intra-ocular pressure associated with two different anaesthetic induction and tracheal intubation techniques were compared (n = 30). after pre-oxygenation, group a received thiopentone (5 mg/kg) followed by suxamethonium (1.5 mg/kg), both within 25 seconds, and group b atracurium (0.5 mg/kg) followed by thiopentone (5 mg/kg) again both within 25 seconds. tracheal intubation occurred after one minute in group a and 2 minutes in group b to allow for full paralysis. in group a intra- ...20113232776
nutrition education-or education in child care? 20164746245
[specificity of autoantibodies discovered in pemphigus and in the bullous variety of duhring-brocq disease]. 20074250902
[effects of different postures on left ventricular function during various trimesters of pregnancy]. 1985261924
[evaluation of various radiographic technics for the diagnosis of periodontal diseases with special reference to the status x unilateral technic].various x-ray techniques (panorama views and intraoral plates) were contrasted and evaluated for diagnostic use in periodontal lesions. in addition the status x-unilateral technique (durner, meyer) was compared to intraoral right angle roentgen states (updergrave) in the examination of 20 patients.1985271590
malignant cystosarcoma phyllodes associated with scirrhous carcinoma of the breast: a case report.the presence of a malignant cystosarcoma phyllodes in one breast and a scirrhous carcinoma in the opposite breast in a premenopausal black woman is described. malignant cystosarcoma phyllodes is uncommon and its rare association with carcinoma of the breast is mentioned.1987204062
sickle cell crises: psychological factors associated with onset. 1989267253
amputation: surgical technique and postoperative management.the decision to amputate should be made with due consideration of the eventual rehabilitation of the patient. with this in mind, general principles of amputation surgery are outlined and specific levels of amputation are described. early post-amputation management has advanced over the last two decades to make rehabilitation goals the more readily reached. these advances are discussed.1991269712
[multiple follicular cyst of familial character]. 1992265282
[multicentric chemodectoma]. 20033020621
relationships among the secretion of acth, gh, and cortisol during the insulin-induced hypoglycemia test in the normal and obese child.the behavior of the secretion of acth, gh, and plasma cortisol during the insulin-induced hypoglycemia test on normal and obese children was studied. the secretion of the above-mentioned hormones was determined by calculating the integrals of the curves. the mean values of the integrals of the plasma cortisol and acth curves do not show any significant differences between the two groups of children. the mean values of the ratios between the integrals of the plasma cortisol and acth curves show a ...2003165219
liver surface characteristics as observed during laparoscopy correlated with biopsy findings. 2003153256
[continuous noninvasive recording of circulatory parameters while performing the valsalva test under elevated ambient pressure].the results are presented which confirm applicability of the valsalva test as a functional exercise for the cardiovascular system while studying changes of the circulation regulation under high ambient pressure. methods of transthoracic tetrapolar impedance plethysmography, electro- and intervalocardiography and mean arterial pressure measurement by means of the oscillometric servosystem are shown as adequate and highly informative under these conditions.20051790814
[health economics--stopgap measures are no decisions. interview by kaj nyman]. 20062095080
chronic subdural haematoma after spinal anaesthesia. case report.in this study an interesting and not frequent case of non-traumatic chronic subdural haematoma after spinal anaesthesia is reported. after a careful review of the cases described in the literature, the authors discussed the physiopathological mechanisms, emphasizing how the break of the bridge veins or of the subarachnoid granulations, the cerebral atrophy and the dehydration are factors which facilitate the appearance of this neurological complication. they suggest that a suitable post-operativ ...20094093803
chronic subdural haematoma after spinal anaesthesia. case report.in this study an interesting and not frequent case of non-traumatic chronic subdural haematoma after spinal anaesthesia is reported. after a careful review of the cases described in the literature, the authors discussed the physiopathological mechanisms, emphasizing how the break of the bridge veins or of the subarachnoid granulations, the cerebral atrophy and the dehydration are factors which facilitate the appearance of this neurological complication. they suggest that a suitable post-operativ ...20094093803
studies on the effects of pharmacological agents on antigen-induced arthritis in balb/c mice.the model of antigen-induced monoarticular arthritis in balb/c mice, originally described by brackertz et al. (1), has been examined with regard to disease pathogenesis and the activities of established antirheumatic agents. the acute phase of the arthritis, up to 7 days after intra-articular (ia) challenge, was characterized by intense polymorphonuclear leukocyte infiltration into the challenged joint, synovial lining cell hypertrophy and hyperplasia, accumulation of mononuclear cells within th ...20102890505
molecular neurobiology--a conference sponsored by the nimh. 20102902686
the endocrine responses to general anesthesia. 20113049390
a 2,4-dichlorophenyl diazonium-based method for total bilirubin without interference from indican in uremic sera.comparison of total bilirubin quantification by a 2,4-dichlorophenyl diazonium method (2,4-dcpd) with a jendrassik-grof type of method showed excellent correlation for randomly selected sera. however, sera from uremic patients on chronic hemodialysis showed a marked positive bias for the 2,4-dcpd result as compared with the jendrassik-grof result. the mean difference was 5.3 mg/l, and resulted in about 20% of the hemodialysis patients having bilirubin values greater than 13 mg/l, the upper limit ...20133924443
a 2,4-dichlorophenyl diazonium-based method for total bilirubin without interference from indican in uremic sera.comparison of total bilirubin quantification by a 2,4-dichlorophenyl diazonium method (2,4-dcpd) with a jendrassik-grof type of method showed excellent correlation for randomly selected sera. however, sera from uremic patients on chronic hemodialysis showed a marked positive bias for the 2,4-dcpd result as compared with the jendrassik-grof result. the mean difference was 5.3 mg/l, and resulted in about 20% of the hemodialysis patients having bilirubin values greater than 13 mg/l, the upper limit ...20133924443
cranial ultrasound and clinical studies in preterm infants.serial ultrasound imaging of the brain was used to determine the ventricular index (vi), and the ratio (vr) of the vi to the cranial hemidiameter during the nursery course and first year post-term in preterm infants of less than 33 weeks gestation. twenty-nine of the infant survivors with no intracranial hemorrhage or major medical complication during their nursery course composed group 1. twenty-two survivors with intracranial hemorrhage unassociated with early ventricular dilation composed gro ...20133881581
efficacy of propranolol therapy after acute myocardial infarction related to coronary arterial anatomy and left ventricular function.the effect of the beta-adrenergic blocking agent propranolol on morbidity and mortality risk after acute myocardial infarction was studied relative to coronary anatomy and left ventricular (lv) ejection fraction in a subset of 406 patients participating in a randomized study of 3,837 patients in the beta blocker heart attack trial (bhat). median follow-up for this subset of patients was 28 months. the mortality rate was 2% (2 of 100) in patients with 2- and 3-vessel coronary artery disease takin ...20133673915
pulmonary toxoplasmoma presenting as obstructive pneumonia. 20133787527
[morbidity and health status of crews and shore personnel of the transit river fleet of the ukraine ssr]. 20144335510
[key points in the neuropathological study of idiopathic parkinsonism]. 20144897052
r.d. laing in post-critical perspective. 20154600314
[radiation protection in dental radiography]. 20154527513
[tendon hyperreflexia in neurologic clinical practice]. 20165004314
molecular characterizations of cytolethal distending toxin produced by providencia alcalifaciens strains isolated from patients with diarrhea.cytolethal distending toxins (cdts), which block eukaryotic cell proliferation by acting as inhibitory cyclomodulins, are produced by diverse groups of gram-negative bacteria. active cdt is composed of three polypeptides--cdta, cdtb, and cdtc--encoded by the genes cdta, cdtb, and cdtc, respectively. we developed a pcr-restriction fragment length polymorphism assay for the detection and differentiation of five alleles of cdtb (cdt-i through cdt-v) in escherichia coli and used the assay to investi ...201222252871
searchdogs bacteria, software that provides automated identification of potentially missed genes in annotated bacterial genomes.we report the development of searchdogs bacteria, software to automatically detect missing genes in annotated bacterial genomes by combining blast searches with comparative genomics. having successfully applied the approach to yeast genomes, we redeveloped searchdogs to function as a standalone, downloadable package, requiring only a set of genbank annotation files as input. the software automatically generates a homology structure using reciprocal blast and a synteny-based method; this is follo ...201424659774
antimicrobial activity of the mycotoxin citrinin obtained from the fungus penicillium citrinum.the mycotoxin citrinin was obtained from the fungus penicillium citrinum. it was tested for it's minimum inhibitory concentration (mic) against some gram positive strains viz. staphylococcus aureus, bacillus pumilus, bacillus subtillis, bacillus cereus, klebsiella pneumoniae, streptococcus pneumoniae, lactobacillus arabinosus and gram negative strains e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis and vibrio cholerae. further the zones o ...200222557053
antimicrobial action of the leaf extract of lagerstroemia parviflora roxb.the benzene extract of the leaves of lagerstroemia paviflora roxb was tested for its minimum inhibitory concentration (mic) against gram positive staphylococcus aureus, bacillus subtilis, bacillus cereus, klebsiella pneumoniae, streptococcus pneumoniae, lactobacillus arabinosus and gram negative strains e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis and vibrio cholerae. further the zones of inhibition produced by the crude extract agains ...200222557054
rational design of reversible inhibitors for trehalose 6-phosphate phosphatases.in some organisms, environmental stress triggers trehalose biosynthesis that is catalyzed collectively by trehalose 6-phosphate synthase, and trehalose 6-phosphate phosphatase (t6pp). t6pp catalyzes the hydrolysis of trehalose 6-phosphate (t6p) to trehalose and inorganic phosphate and is a promising target for the development of antibacterial, antifungal and antihelminthic therapeutics. herein, we report the design, synthesis and evaluation of a library of aryl d-glucopyranoside 6-sulfates to se ...201728192710
synthesis of newer 1,2,3-triazole linked chalcone and flavone hybrid compounds and evaluation of their antimicrobial and cytotoxic activities.the present study was carried out in an attempt to synthesize a new class of antimicrobial and antiplasmodial agents by copper catalyzed click chemistry to afford 25 compounds 10-14(a-e) of 1,4-disubstituted-1,2,3-triazole derivatives of chalcones and flavones. the structures of the newly synthesized compounds were established by elemental analysis, ir, (1)h nmr, (13)c nmr and mass spectral data. the newly synthesized compounds were evaluated for their antibacterial activity against gram positiv ...201626922227
design, synthesis and antimicrobial activity of novel benzothiazole analogs.in an attempt to design and synthesize a new class of antimicrobials, dialkyne substituted 2-aminobenzothiazole was reacted with various substituted aryl azides to generate a small library of 20 compounds (3a-t) by click chemistry. structures of the newly synthesized compounds were established on the basis of spectral data. these compounds were screened for their antibacterial activity against gram+ bacteria (staphylococcus aureus and enterococcus faecalis), gram- bacteria (salmonella typhi, esc ...201323567952
antimicrobial assay and genetic screening of selected freshwater cyanobacteria and identification of a biomolecule dihydro-2h-pyran-2-one derivative.explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and methicillin-resistant staphylococcus aureus (mrsa) strains) drug resource using bioassay, nrps (non-ribosomal polypeptide synthetase) and pks (polyketide synthase) genes, as well as in silico approach.201728004519
purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a bangladeshi cultivar of potato (solanum tuberosum).a new chitin-binding lectin was purified from a bangladeshi cultivar 'deshi' of potato (solanum tuberosum l.) through anion-exchange and affinity chromatographies using a chitin column. sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) showed the molecular mass of the lectin as 20,000 daltons. this molecular mass was almost half of the molecular masses of chitin-binding lectins derived from other potatoes. the lectin showed both bactericidal and growth-inhibiting activities a ...201424980018
analgesic, antibacterial and central nervous system depressant activities of albizia procera leaves.to ascertain analgesic, antibacterial and central nervous system (cns) depressant activities of ethyl acetate, dichloromethane and carbon tetrachloride fractions of methanol extract of albizia procera (a. procera) leaves.201425182551
development of 23 individual taqman® real-time pcr assays for identifying common foodborne pathogens using a single set of amplification conditions.most of the acute intestinal diseases are caused by foodborne pathogens with infants and elderly people being at major risk. the aim of this study was to develop a procedure to simultaneously detect 20 foodborne pathogens in complex alimentary matrices such as milk, cheese and meat. the list of targets include, among the others, listeria spp., salmonella spp., shigella spp., escherichia coli spp., campylobacter spp., clostridium spp. and staphylococcus aureus. the accuracy of detection was deter ...201424929880
antibacterial activities and in vitro anti-inflammatory (membrane stability) properties of methanolic extracts of gardenia coronaria leaves.this work is carried out with gardenia coronaria leaves that belong to the family rubiaceae, which is a small-to-medium-sized but tall, deciduous tree, 7.6-9 m high on an average. leaves are used for the treatment of rheumatic pain and bronchitis. the leaf of the plant consists of coronalolide, coronalolic acid, coronalolide methyl ester, ethyl coronalolate acetate triterpenes (secocycloartanes), and so forth. methanol extract from the leaves of gardenia coronaria was completely screened for mem ...201424695677
antimicrobial potency of the leaf - stalk extract of curcuma longa (linn).the methanolic extract of the leaf-stalk of curcuma longa linn, was tested for its minimum inhibitor concentration (mic) against gram positive-staphylococcus aureus, bacillus pumilus, bacillus subtilis, klebsiella pnemoniae, bacillus cereus, streptococcus pneumoniae, lactobacillus arabinosus and gram negative e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis, and vibrio cholerae strains, further, the ones of inhibition produced by the crude ...200022557005
evaluation of food protective property of five natural products using fresh-cut apple slice model.the present study evaluated the antioxidant (aa), antimicrobial and preservation effects of five plant derived natural products viz., rosmarinic acid (ra), p-coumaric acid (pca), trans-cinnamic acid (tca), hydroxyphenyllactic acid (hpa) and caffeic acid (ca) along with synthetic compounds (ascorbic acid, gallic acid, citric acid and bha) on fresh cut apple slices. antimicrobial efficacy of these compounds against bacillus licheniformis, pseudomonas vulgaris, shigella boydii, salmonella typhi, st ...201222530437
a genomic island in salmonella enterica ssp. salamae provides new insights on the genealogy of the locus of enterocyte effacement.the genomic island encoding the locus of enterocyte effacement (lee) is an important virulence factor of the human pathogenic escherichia coli. lee typically encodes a type iii secretion system (t3ss) and secreted effectors capable of forming attaching and effacing lesions. although prominent in the pathogenic e. coli such as serotype o157:h7, lee has also been detected in citrobacter rodentium, e. albertii, and although not confirmed, it is likely to also be in shigella boydii. previous phyloge ...201222860002
stress tolerant virulent strains of cronobacter sakazakii from food.cronobacter sakazakii is considered as an emerging foodborne pathogen. the aim of this study was to isolate and characterize virulent strains of cronobacter sakazakii from food samples of bangladesh.201425723712
structure and gene cluster of the o-antigen of enterobacter cloacae g3421.the o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of enterobacter cloacae g3421 and studied by sugar analysis along with 1d and 2d (1)h and (13)c nmr spectroscopy. in addition, partial solvolysis with anhydrous trifluoroacetic acid was applied, which cleaved selectively the α-l-rhamnopyranosidic linkages. the following structure of the branched hexasaccharide repeating unit was established. the o-polysaccharide studied shares the β-l-rhap-(1→4)-α-l-rhap-(1→2)-α ...201627131290
escherichia albertii, a newly emerging enteric pathogen with poorly defined properties.escherichia albertii is a newly emerging enteric pathogen that has been associated with sporadic infections among humans and birds. selected coliform isolates were screened for allelic variation in 2 housekeeping genes (lysp and mdh) specific for e. albertii. the 48 strains that were identified as e. albertii were tested for 15 virulence markers and biochemical and serogical properties. all e. albertii strains were non-motile, fermented d-glucose (with gas), d-mannitol, and d-mannose, but failed ...201323938305
structure and genetics of the o-antigens of escherichia coli o182-o187.o-polysaccharides (opss) were obtained by mild acid degradation of the lipopolysaccharides of escherichia coli o182-o187, and their structures were established by sugar analysis, smith degradation, and (1)h and (13)c nmr spectroscopy. in addition to the monosaccharides that occur often in e. coli opss (d-glc, d-gal, d-man, d-glcnac, d-galnac, d-glca, l-fuc, d-rib), a number of less common components were identified as the ops constituents, including 2-acetamido-2-deoxy-l-quinovose and 4-deoxy-4- ...201627710814
bacterial assessment of food handlers in sari city, mazandaran province, north of iran.food handlers with poor personal hygiene could be potential sources of infection due to pathogenic bacteria. this study was designed to determine the prevalence of bacterial infestation among the food handlers attending the public health center laboratory in sari, northern iran for annual check-up. this study was performed from september 2013 to august 2014. stool samples, fingernail specimens of both hands and nasal swabs were collected from 220 male and female food handlers of different jobs, ...201727435639
shigella diversity and changing landscape: insights for the twenty-first century.shigella is a pathovar of escherichia coli comprising four groups, shigella flexneri, shigella sonnei, shigella dysenteriae, and shigella boydii, each of them, with the exception of s.sonnei, comprising several serotypes. shigella accounts for the majority of dysentery causing infections occurring world-wide each year. recent advancements in the shigella field have led to a better understanding of the molecular mechanisms underlying host epithelial cell invasion and immune cell function manipula ...201627148494
structure and genetics of the o-antigen of escherichia coli o169 related to the o-antigen of shigella boydii type 6.the o-polysaccharide (o-antigen) of escherichia coli o169 was studied by sugar analysis along with 1d and 2d (1)h and (13)c nmr spectroscopy. the following structure of the branched hexasaccharide repeating unit was established: [formula: see text] the o-polysaccharide of e. coli o169 differs from that of shigella boydii type 6 only in the presence of a side-chain glucose residue. a comparison of the o-antigen biosynthesis gene clusters between the galf to gnd genes in the genomes of the two bac ...201526232763
role of porin proteins in acquisition of transferrin iron by enteropathogens.acquisition of iron from key innate immune defence proteins such as transferrin (tf) and lactoferrin is an important mechanism by which pathogenic bacteria obtain essential iron for growth within their host. bacterial species that do not produce siderophores often use specific tf-binding proteins, the best characterized being the neisseriaceae-type tf-binding proteins tbpa and tbpb. previous work from our laboratory has shown that siderophore-producing enteric species such as escherichia coli al ...201324089578
oxalis corniculata (oxalidaceae) leaf extract exerts in vitro antimicrobial and in vivo anticolonizing activities against shigella dysenteriae 1 (nt4907) and shigella flexneri 2a (2457t) in induced diarrhea in suckling mice.in this study, the extract of a green leafy vegetable oxalis corniculata (oxalidaceae) was evaluated for its in vitro antibacterial and in vivo anti colonizing effect against common intestinal pathogenic bacteria. methanolic extract (80%) of oxalis corniculata (oxalidaceae) leaf contained a polyphenol content of 910 mg gallic acid equivalent per gram of dry weight and the yield was 8%. the flavonoid content was 2.353 g quercetin equivalent per 100 g of the extract. in vitro studies indicated tha ...201323909903
patterning bacterial communities on epithelial cells.micropatterning of bacteria using aqueous two phase system (atps) enables the localized culture and formation of physically separated bacterial communities on human epithelial cell sheets. this method was used to compare the effects of escherichia coli strain mg1655 and an isogenic invasive counterpart that expresses the invasin (inv) gene from yersinia pseudotuberculosis on the underlying epithelial cell layer. large portions of the cell layer beneath the invasive strain were killed or detached ...201323785519
development of a dna macroarray for simultaneous detection of multiple foodborne pathogenic bacteria in fresh chicken meat.a dna macroarray was developed to provide the ability to detect multiple foodborne pathogens in fresh chicken meat. probes targeted to the 16s rrna and genus- and species-specific genes, including fimy, ipah, prfa, and uspa, were selected for the specific detection of salmonella spp., shigella spp., listeria monocytogenes, and escherichia coli, respectively. the combination of target gene amplification by pcr and a dna macroarray in our system was able to distinguish all target bacteria from pur ...201323754709
recombinant heat shock protein 60 (hsp60/groel) of salmonella enterica serovar typhi elicits cross-protection against multiple bacterial pathogens in mice.heat shock proteins (hsps) or stress proteins are recognized as protective antigens against a wide range of bacterial diseases. conservation of hsps across different life forms also appears to contribute to the antigenicity of these proteins. due to their high sequence homology, there exists an immunological cross-recognition between different bacterial species. in the present study, we evaluated the efficacy of recombinant groel of salmonella enterica serovar typhi as a vaccine candidate agains ...201323474314
in vitro development and analysis of escherichia coli and shigella boydii azithromycin-resistant mutants.the aim of this study was to develop and analyze in vitro azithromycin (azm)-resistant mutants of escherichia coli and shigella boydii. three clinical isolates of e. coli and one s. boydii isolated from feces samples collected from children under 5 years of age with diarrhea in lima, peru were inoculated onto mueller-hinton plates containing increasing serial dilutions of azm ranging from their specific minimal inhibitory concentration (2 or 4 mg/l) to 64 mg/l. from these plates, 16 azm-resistan ...201323176550
evidence of interspecies o antigen gene cluster transfer between shigella boydii 15 and escherichia fergusonii.an environmental bacterial isolate, iso10, previously found to show serological cross-reactivity with type-specific shigella boydii 15 antisera was subjected to further molecular and serological analyses that revealed interspecies transfer of the o antigen gene cluster. western blot analysis of iso10 cell surface extracts and purified lipopolysaccharides demonstrated strong cross-reactivity with s. boydii 15-specific monovalent antisera and a lipopolysaccharide gel banding profile similar to tha ...201223030400
chemical and antimicrobial studies on the essential oil from salvia santolinifolia boiss.in view of the reputation of genus salvia in folklore medicine and its abundance in our region, the chemical composition and antimicrobial activity of essential oil from s. santolinifolia boiss. was analyzed. chemical analysis, using gas chromatography and gas chromatography mass spectrometry, retention indices and c-13 nuclear magnetic resonance spectroscopy has resulted in identification of 116 constituents, comprising about 97% of the total constituents. out of these 116, 78 constituents are ...201323261726
in vitro probiotic characterization of lactobacillus strains from fermented radish and their anti-adherence activity against enteric pathogens.in this study, we evaluated the probiotic properties of lactobacillus plantarum, lactobacillus pentosus, and lactobacillus fermentum strains isolated from fermented radish. all the strains survived the simulated oro-gastrointestinal transit condition and showed significantly higher adherence to caco-2 cells compared with the probiotic strain lactobacillus rhamnosus gg. the strains showed broad-spectrum antimicrobial activity, autoaggregation, and coaggregation capacity with pathogens. furthermor ...201526382558
virulotyping of shigella spp. isolated from pediatric patients in tehran, iran.shigellosis is a considerable infectious disease with high morbidity and mortality among children worldwide. in this survey the prevalence of four important virulence genes including ial, ipah, set1a, and set1b were investigated among shigella strains and the related gene profiles identified in the present investigation, stool specimens were collected from children who were referred to two hospitals in tehran, iran. the samples were collected during 3 years (2008-2010) from children who were sus ...201728218003
shigellosis in nepal: 13 years review of nationwide surveillance.shigella is a major cause of gastroenteritis especially in children. in developing countries, the incidence is frequent and results are often life threatening. changing epidemiology and emerging antibiotic resistance warrants continuous monitoring of susceptibility. the present study highlights the changing epidemiology and drug resistance patterns of shigella isolated at different hospitals of nepal over a period of 13 years (jan. 2003-dec. 2015).201627814742
the first report of a fully sequenced resistance plasmid from shigella boydii.the purpose of this study was to characterize mechanisms of plasmid-mediated antimicrobial resistance in shigella boydii. s. boydii strain 2246 with resistance to ciprofloxacin, ceftriaxone and azithromycin was isolated from a human case of watery diarrhea in a chinese public hospital. resistance in strain 2246 to ceftriaxone and azithromycin was attributable to the presence of blactx-m-14, and erm(b) and mph(a), respectively, which were co-located on a multidrug-resistant (mdr) plasmid p2246-ct ...201627766094
a review on sero diversity and antimicrobial resistance patterns of shigella species in africa, asia and south america, 2001-2014.shigella, gram negative bacterium, is responsible for shigellosis/bacillary dysentery. it is a global concern although it predominates in developing countries. these are shigella dysenteriae, shigella flexneri, shigella boydii and shigella sonnei. drug resistance by shigella species is another headache of the world. therefore; this study aimed to review distribution of shigella serogroups and their antimicrobial patterns carried out in africa, asia and south america.201627576729
molecular characterisation of quinolone-resistant shigella strains isolated in tehran, iran.over the past few years, the number of shigella strains resistant to nalidixic acid has increased and has made the selection of effective antimicrobial therapy more difficult. the purpose of this study was to investigate the molecular mechanism of quinolone resistance in shigella strains. shigella strains isolated from 1100 diarrhoeal patients in tehran, iran, were assessed for their susceptibility to nalidixic acid prior to pcr-rflp and sequence analysis of their quinolone resistance genes. amo ...201627436462
genome diversity of shigella boydii.italic! shigella boydiiis one of the four italic! shigellaspecies that causes disease worldwide; however, there are few published studies that examine the genomic variation of this species. this study compares genomes of 72 total isolates; 28 italic! s. boydiifrom bangladesh and the gambia that were recently isolated as part of the global enteric multicenter study (gems), 14 historical italic! s. boydiigenomes in the public domain and 30 italic! escherichia coliand italic! shigellareference geno ...201627056949
antibacterial activity of a novel fatty acid (14e, 18e, 22e, 26e)-methyl nonacosa-14, 18, 22, 26 tetraenoate isolated from amaranthus spinosus.amaranthus spinosus linn. (amaranthaceae), commonly known as ''spiny pigweed'', is used in both indian traditional system and folk medicine for treatment of infectious diseases for a long time in several traditional herbal medicinal preparations. a novel fatty acid [(14e, 18e, 22e, 26e)-methyl nonacosa-14, 18, 22, 26 tetraenoate] is the major metabolite present.201627043360
development of a cost-effective vaccine candidate with outer membrane vesicles of a tola-disrupted shigella boydii strain.our previous studies on outer membrane vesicles based vaccine development against shigellosis, revealed the inability of shigella to release significant amount of vesicles naturally, during growth. disruption of tola, one of the genes of the tol-pal system of gram negative bacterial membrane, has increased the vesicle release rate of a shigella boydii type 4 strain to approximately 60% higher. we also noticed the vesicles, released from tola-disrupted strain captured more ompa protein and lipopo ...201626878295
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