| cross-reactions in legionella antisera with bordetella pertussis strains. | while preparing slide agglutination test antisera and immunofluorescence conjugates for the identification of legionella species and serogroups, we found that several of the reagents cross-reacted with bordetella pertussis strains. to determine the extent of this problem and to estimate the specificity of legionella reagents, we tested slide agglutination test antisera against 22 species and 35 serogroups with 92 bacterial strains representing 19 genera. the only cross-reactions observed were wi ... | 1987 | 2883198 |
| [serotype and drug susceptibility of bordetella pertussis and bordetella parapertussis isolated from 1975 to 1985 in japan]. | | 1987 | 2883241 |
| correlation between growth rate and loss of histamine-sensitizing factor during antigenic modulation in bordetella pertussis. | the pattern of loss of histamine-sensitizing factor (hsf) during antigenic modulation of bordetella pertussis in hornibrook medium was examined. the aim was to determine the possible underlying mechanism involved in modulation. normal (x-mode) b. pertussis cells were grown in hornibrook medium in which 0.5% (w/v) nacl had been replaced with 0.5% (w/v) mgso4. 7h2o (c-medium). at various time intervals during growth, the viable cell numbers and optical densities of both cultures in the x- and c-me ... | 1987 | 2883559 |
| alteration of intracellular camp levels and beating rates of cultured chick cardiac cells by bordetella pertussis adenylate cyclase. | bordetella pertussis, the pathogen responsible for whooping cough, releases a soluble calmodulin-sensitive adenylate cyclase into its culture medium which enters several different types of animal cells and elevates intracellular camp. in this study, the influence of b. pertussis adenylate cyclase on intracellular camp levels of cultured chick cardiac cells and the beating rates of chick cardiac cell aggregates was examined. treatment with b. pertussis adenylate cyclase caused up to a 60-fold inc ... | 1987 | 2883570 |
| a method for purification of bacterial r-type lipopolysaccharides (lipooligosaccharides). | a new gel filtration method was developed for purification of r-type lipopolysaccharides (lipooligosaccharides) from some nonenteric gram-negative bacteria, including neisseria meningitidis, haemophilus influenzae, and bordetella pertussis. these wild-type lipooligosaccharides are poorly extractable by the phenol-chloroform-ether extraction method of c. galanos, o. luderitz, and o. westphal [1969) eur. j. biochem. 9, 245-249) and therefore a new procedure was developed for their isolation. the l ... | 1987 | 2883909 |
| the construction of a cloning vector designed for gene replacement in bordetella pertussis. | we report here the construction of a plasmid cloning vector, prtp1, designed to facilitate exchange of cloned and chromosomal alleles of the human bacterial pathogen bordetella pertussis. prtp1 provides the ability to successively select two homologous recombination events within the cloned sequences. the first is by selection for maintenance of the ampicillin-resistance gene on the plasmid which is unable to replicate autonomously after transfer via conjugation. the second selection, via strept ... | 1986 | 2884169 |
| maintenance of meiotic arrest in isolated rat oocytes by the invasive adenylate cyclase of bordetella pertussis. | rat oocytes resume meiosis spontaneously in vitro within 3 h after their isolation from the ovarian follicles. we report here that the spontaneous maturation of isolated rat oocytes is preceded by a drop in intracellular levels of cyclic adenosine 3',5'-monophosphate (camp). further experiments were carried out to examine the possible correlation between the meiotic status and camp levels within the oocyte. to challenge rat cumulus-free oocytes to generate camp, bypassing their own adenylate cyc ... | 1987 | 2885039 |
| recovery of bordetella pertussis from four kinds of swabs. | calcium alginate, dacron, rayon and cotton wool tipped swabs, in combination with charcoal horse blood transport medium with cephalexin, were compared with regard to their ability to maintain viability of bordetella pertussis. calcium alginate proved to be the most suitable swab material. a field trial confirmed the results of the laboratory study. | 1987 | 2885191 |
| comparison of throat and nasopharyngeal swab specimens for culture diagnosis of bordetella pertussis infection. | during a 9-month period, we evaluated the relative sensitivity of throat and nasopharyngeal swab cultures for isolation of bordetella pertussis. of 38 pertussis cases, 36 (95%) had positive nasopharyngeal cultures, while only 16 of 36 (44%) had positive throat cultures. there were no cases of nasopharyngeal-negative, throat-positive cultures. the sensitivity of the direct fluorescent-antibody test was 70% when compared with culture. | 1987 | 2885339 |
| erythromycin in the treatment of pertussis: a study of bacteriologic and clinical effects. | in an open randomized study 17 patients with a positive culture for bordetella pertussis were treated for 10 days with erythromycin (50 mg/kg/day divided in 2 doses). the bacterium could not be isolated during therapy and in only one patient was it isolated 5 days after cessation of treatment. in comparison b. pertussis was isolated 10 and 15 days after diagnosis from 10 and 4 patients, respectively, of a group of 21 untreated controls. the treated group developed significantly fewer whoops than ... | 1987 | 2885802 |
| elisa for total ige and igg detection in rat serum. changes with immunization or adjuvants. | total ige and igg serum levels were measured in rats treated with egg albumin (ea), bordetella pertussis (bp) and aluminium hydroxide gel (alum), and in rats administered with bordetella pertussis or alum alone. two elisa micromethods have been developed to measure ige and igg changes. immunoglobulin serum levels were evaluated 14 days after immunization. the highest ige levels were obtained after sensitization with ea suspended in alum s.c. and administered with bp i.p. the ige production induc ... | 1987 | 2886032 |
| the invasive adenylate cyclase of bordetella pertussis. properties and penetration kinetics. | bordetella pertussis, the causative organism of whooping cough, produces a calmodulin-sensitive adenylate cyclase. confer & eaton [(1982) science 217, 948-950] have shown that an extract from b. pertussis increases intracellular cyclic amp levels in neutrophils and suggested that this increase is caused by the bacterial adenylate cyclase which penetrates these cells. we demonstrate in the present study that adenylate cyclase activity in lysates from lymphocytes exposed to a partially purified pr ... | 1987 | 2886119 |
| the invasive adenylate cyclase of bordetella pertussis. intracellular localization and kinetics of penetration into various cells. | the penetration of bordetella pertussis adenylate cyclase into various mammalian cells exhibits similar kinetics; the accumulation of both intracellular cyclase activity and cyclic amp is rapid, reaching constant levels after 15-60 min of incubation. the kinetics of enzyme penetration into turkey erythrocytes is different; cyclase activity and cyclic amp accumulate linearly and do not reach constant levels even after 6 h of incubation. in the preceding paper [friedman, farfel & hanski (1987) bio ... | 1987 | 2886120 |
| susceptibility of bordetella pertussis and bordetella parapertussis to 24 antibiotics. | the susceptibility of bordetella pertussis (28 strains) and bordetella parapertussis (6 strains) to 24 antibiotics (penicillin and cephalosporin derivatives, erythromycin, josamycin, cotrimoxazole, imipenem, aztreonam and fosfomycin) was studied by means of the agar dilution method using charcoal horse blood agar. piperacillin and mezlocillin showed the highest activity (mic 0.0039-0.00781 micrograms/ml) against b. pertussis while b. parapertussis was most susceptible to piperacillin (0.03125-0. ... | 1987 | 2886295 |
| the potentiating effects of phorbol ester on acth-, cholera toxin-, and forskolin-induced camp production by cultured bovine adrenal cells is not mediated by the inactivation of alpha subunit of gi protein. | phorbol ester (pma) potentiates acth-induced camp production by both fresh isolated and 7-day-old cultured adrenal cells, but the effect on cultured cells was greater than in fresh cells. in cultured cells the potentiating effects of pma were dose-dependent and were observed at each effective dose of acth without modification of the ed50 for this hormone. these effects of pma do not seem to be exerted through a modification of the alpha subunit of gi since pretreatment of the cells with bordetel ... | 1987 | 2887161 |
| major fragment of soluble peptidoglycan released from growing bordetella pertussis is tracheal cytotoxin. | bordetella pertussis is known to release a factor which promotes the loss of ciliated respiratory epithelium and copurifies with a soluble peptidoglycan (pg) fragment termed tracheal cytotoxin (tct). the objective of this study was to determine whether pertussis organisms turn over and release pg derivatives in addition to tct. b. pertussis tohama (phase iii) was grown in liquid stainer-scholte medium containing [3h]diaminopimelic acid (dap) to label pg specifically, washed to remove free label, ... | 1987 | 2887513 |
| structure of bordetella pertussis peptidoglycan. | bordetella pertussis tohama phases i and iii were grown to the late-exponential phase in liquid medium containing [3h]diaminopimelic acid and treated by a hot (96 degrees c) sodium dodecyl sulfate extraction procedure. washed sodium dodecyl sulfate-insoluble residue from phases i and iii consisted of complexes containing protein (ca. 40%) and peptidoglycan (60%). subsequent treatment with proteinase k yielded purified peptidoglycan which contained n-acetylglucosamine, n-acetylmuramic acid, alani ... | 1987 | 2887547 |
| r-plasmid-mediated chromosome mobilization in bordetella pertussis. | antibiotic-resistant and auxotrophic mutants of bordetella pertussis were isolated. these were used as recipients for the uptake from escherichia coli of broad-host-range r plasmids r68.45, rp1, and rp1 and rp4 carrying transposons tn501 and tn7 respectively. b. pertussis transconjugants from these crosses were used as donors to mobilize strr, nalr, thr+ and gly+ chromosomal markers to b. pertussis or to b. parapertussis recipient strains. the frequency of plasmid transfer varied and depended on ... | 1986 | 2887625 |
| complementation of mutations in escherichia coli and bordetella pertussis by b. pertussis dna cloned in a broad-host-range cosmid vector. | a gene library of bordetella pertussis dna was constructed in escherichia coli using the broad-host-range cosmid vector plafr1. the average insert size was 24.9 kb. from 500 members of the gene library, clones were identified which complemented trpe, glna and thr- mutations in e. coli but none which complemented trpd, trpc, trpb, trpa, proa or leu- mutations. four clones were identified which complemented trpe in e. coli. anthranilate synthase activity was detected in a trpe strain only when it ... | 1986 | 2887629 |
| [characteristics of the biological properties of a cell-free pertussis preparation]. | the biological properties of bordetella pertussis antigenic complex, obtained by a technologically simple method from the medium used for the cultivation of b. pertussis, were studied. the preparation was characterized by pronounced hemagglutinating activity, toxicity, histamine-sensitizing and leukocytosis-stimulating activity and produced a cytopathogenic effect on the culture of chinese hamster ovary cells. the detoxified preparations showed pronounced protective activity in experiments on th ... | 1987 | 2888247 |
| [cytopathogenic action of intact and antibiotic-altered populations of bordetella pertussis on a human amnion cell culture]. | cytopathogenic and adhesive action of intact b. pertussis population and population changed under the effect of antibiotics was studied comparatively. it was shown that the level and character of the changes in the cell culture of line fl human amnion depended on the antibiotic used in the experiments. populations of b. pertussis changed under the effect of tetracycline had the highest cytopathogenic and adhesive activity, while the activity of the population changed under the effect of erythrom ... | 1987 | 2888430 |
| construction and characterization of bordetella pertussis toxin mutants. | pertussis toxin is one of the major virulence determinants produced by bordetella pertussis. the dna encoding the structural genes for pertussis toxin was cloned in escherichia coli, and pertussis toxin subunit s4 was expressed under the control of the tac promoter. mutations were introduced into the cloned toxin genes, and a conjugative shuttle vector system was devised for delivering the mutations from e. coli back into b. pertussis. the mutations were introduced by allelic exchange into the c ... | 1987 | 2888733 |
| inhibition of monocyte oxidative responses by bordetella pertussis adenylate cyclase toxin. | bordetella pertussis and the other bordetella species produce a novel adenylate cyclase toxin which enters target cells to catalyze the production of supraphysiologic levels of intracellular cyclic adenosine monophosphate (camp). in these studies, dialyzed extracts from b. pertussis containing the adenylate cyclase toxin, a partially purified preparation of adenylate cyclase toxin, and extracts from transposon tn5 mutants of b. pertussis lacking the adenylate cyclase toxin, were used to assess t ... | 1987 | 2888823 |
| isolation of a repeated dna sequence from bordetella pertussis. | a repeated dna sequence in the genome of bordetella pertussis has been demonstrated. at least 20 copies of this sequence could be observed in either bamhi or ecori restriction enzyme digests of chromosomal dna; fragments carrying the repeated dna sequence ranged in size from about 1.5 to 20 kbp. the repeated dna sequence was cloned from two separate regions of the b. pertussis genome, as shown by restriction enzyme site maps of the two clones and by hybridization studies. a small number of diffe ... | 1987 | 2888834 |
| interaction of lactoferrin and transferrins with the outer membrane of bordetella pertussis. | bordetella pertussis was able to grow in vitro under conditions where the only iron present was bound to the iron-binding proteins ovotransferrin, transferrin or lactoferrin. under these conditions the bacteria produced neither hydroxamate nor phenolate-catecholate siderophores to assist in the procurement of iron. examination of b. pertussis outer-membrane preparations by sds-page and immunoblotting showed that the iron-binding protein ovotransferrin was bound directly to the bacterial surface. ... | 1987 | 2888836 |
| a model for experimental asthma: provocation in guinea-pigs immunized with bordetella pertussis. | guinea-pigs were sensitized with killed bordetella pertussis (3.85 x 10(11) cells . kg-1). the presence of the immediate type of immune response was verified by passive cutaneous anaphylaxis (pca). in anaesthetized spontaneously-breathing guinea-pigs respiration rate, tidal volume, minute ventilation, dynamic lung resistance and dynamic lung elastance were estimated on the basis of airflow velocity and pressure in the oesophagus. after provocation by a single intravenous injection of the killed ... | 1987 | 2889487 |
| increase in intradermal vascular permeability caused by pertussis toxin from bordetella pertussis. | rabbits that were injected intradermally with pertussis toxin (pt), produced from bordetella pertussis, showed slight edema and erythema at the injection sites, but not hemorrhage nor necrosis. the edema lesions were stained blue by the intravenous injection of pontamine sky blue 6b dye, suggesting that pt caused increased vascular permeability, similarly to the permeability factor (pf) of cholera toxin. the reaction of the pf of pt could be determined by measuring the diameter of the blue area. ... | 1987 | 2890084 |
| acellular pertussis vaccine prepared by a simple extraction and toxoiding procedure. | an extract containing predominantly pertussis toxin (pt) and filamentous haemagglutinin (fha) was obtained from culture supernates of bordetella pertussis by a single-step procedure using dye-ligand chromatography. the pathophysiological activities associated with the pertussis toxin component were removed by treatment with a water-soluble carbodiimide. the product was stable at 4 degrees c, was non-toxic for mice, induced high levels of igg antibodies to both pt and fha in vaccinated animals as ... | 1987 | 2890244 |
| [mitogenic action of the liquid phase of a microbial suspension of bordetella pertussis]. | the suspension of b. pertussis cells in 0.15 m nac1 solution, used for the preparation of corpuscular pertussis vaccine contains components loosely bound to microbial cells and producing pronounced mitogenic effect on mouse splenocytes at a concentration of 10 micrograms/ml. the mitogenic activity of b. pertussis is due to complex substances (lipopolysaccharide, protein, nucleic acids) with a wide range of molecular weights (70,000 to greater than 400,000). the mitogenic factor showing no leukoc ... | 1987 | 2890249 |
| [adhesion of bordetella pertussis]. | | 1987 | 2890250 |
| [possible serodiagnosis of pertussis infection by an immunoenzyme method]. | the sera of children and adults with a history of pertussis, as well as the sera of children immunized in three injections, have been studied in the enzyme immunoassay. the levels of antibodies to bordetella pertussis protein and lipopolysaccharide, and to disintegrated b. pertussis cells have been determined; a serum titer of 1:1,600 and higher is considered as a criterion for the serological diagnosis of pertussis. | 1987 | 2891232 |
| [use of specific bordetella pertussis antibodies in immunoenzyme analysis for detecting the pertussis antigen]. | the competitive eia technique with the use of peroxidase-labeled b. pertussis antigen has been developed. the data obtained in our investigations suggest the possibility of using this technique for the detection of b. pertussis antigen in faucial smears obtained from patients. | 1987 | 2891233 |
| efficacy of enzyme-linked immunosorbent assay for rapid diagnosis of bordetella pertussis infection. | we examined the diagnostic efficacy of an enzyme-linked immunosorbent assay (elisa) for class-specific antibodies to bordetella pertussis in acute-phase sera collected from 1,240 patients with suspected pertussis. a total of 833 serum specimens (67%) yielded positive results. the proportion of positive results increased to 77% if a second (convalescent-phase) serum was also tested. by comparison, a bacterial agglutination test for b. pertussis antibodies was positive in only 21% of acute-phase s ... | 1987 | 2891724 |
| differential effect of bordetella pertussis on experimental posterior uveitis in the black-hooded lister rat. | the effect of an additional adjuvant, bordetella pertussis, on the clinical and histopathologic features of experimental autoimmune uveitis in black-hooded lister rats was investigated. disease was induced by a single footpad injection of purified retinal s-antigen in freund's complete adjuvant. in those animals that did not receive b pertussis the clinical features were those of a retinal vasculitis with disc edema, periphlebitis, and deep retinal infiltrates. in contrast, animals that received ... | 1988 | 2892482 |
| non-toxigenic mutants of "bordetella pertussis". | | 1986 | 2892505 |
| purification and immunological characterization of bacterial and secreted adenylate cyclase of "bordetella pertussis". | | 1986 | 2892509 |
| cloning of a coding sequence of "bordetella pertussis" filamentous hemagglutinin gene. | | 1986 | 2892511 |
| experimental models for testing "bordetella pertussis" virulence and immunogenicity. | | 1986 | 2892512 |
| protective activities of "bordetella pertussis" tn5 mutants in mice. | | 1986 | 2892513 |
| susceptibility of bordetella pertussis to five quinolone antimicrobic drugs. | five quinolone antimicrobic agents were tested to determine the mean inhibitory concentration (mic) of each of 17 clinical strains of bordetella pertussis by the agar dilution method. ciprofloxacin demonstrated the best in vitro activity with an mic90 of 0.06 microgram/ml. norfloxacin, ofloxacin and enoxacin were also highly active with mic90s of 0.25, 0.25, and 0.5, respectively. cinoxacin was only moderately active (mic90 4.0 microgram/ml). | 1987 | 2892608 |
| trypanosoma cruzi: immune response in mice immunized with parasite antigens. | the humoral and cellular immune responses were studied in mice immunized with flagellar fraction (f), f plus bordetella pertussis as adjuvant (f-bp), and microsomal (mc) subcellular fractions from the epimastigote forms of trypanosoma cruzi. the immune response was studied before and after the challenge with 50 bloodstream forms of t. cruzi, tulahuén strain. the immunization with f-bp, but not with mc or f and bp separately, protected mice, in terms of parasitemia and mortality, from the challen ... | 1988 | 2892694 |
| effect of bordetella pertussis adjuvant on parasite-specific ige response in paragonimus ohirai-infected rats. | parasite-specific ige response in rats infected with metacercariae of the lung fluke, paragonimus ohirai, was enhanced about 8 times by bordetella pertussis adjuvant. in rats infected by intraperitoneal transplantation of adult worms, the adjuvant markedly increased the usually low or absent parasite-specific ige response. the most effective time of the adjuvant administration was different between the two modes of infections. | 1988 | 2892799 |
| agglutinating monoclonal antibodies that specifically recognize lipooligosaccharide a of bordetella pertussis. | monoclonal antibodies that specifically agglutinate strains of bordetella pertussis having serotype 1 agglutinogen were uniquely reactive with the electrophoretically slow-migrating a form of lipooligosaccharide. these monoclonal antibodies should be useful for the structural analysis of b. pertussis lipooligosaccharide and for the establishment of a better-defined serogroup for bordetella species. | 1988 | 2893776 |
| interaction of bordetella pertussis adenylate cyclase with calmodulin. identification of two separated calmodulin-binding domains. | the structural organization of bordetella pertussis adenylate cyclase was examined by limited proteolysis with trypsin and/or cross-linking with azido-calmodulin a photoactivable derivative of its activator, calmodulin (cam). adenylate cyclase (which consists of three structurally related peptides of 50, 45, and 43 kda as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) formed a 1:1 complex with cam or azido-cam. cam-bound adenylate cyclase was cleaved by trypsin into two sep ... | 1988 | 2893792 |
| production and characterization of monoclonal antibodies directed against bordetella pertussis lipopolysaccharide. | hybrid cell lines producing monoclonal antibodies against bordetella pertussis lipopolysaccharide (lps) were established. the specificity of the antibodies was ascertained by enzyme-linked immunosorbent assay (elisa) and elisa-inhibition experiments with lps and delipidated polysaccharide fragments (ps-1 and ps-2) prepared from b. pertussis lps. monoclonal antibody 9-1-h5 reacted with b. pertussis lps only, whereas monoclonal antibodies 6-4-h6 and 9-2-a8 reacted with ps-1 and ps-2 as well as b. ... | 1988 | 2893806 |
| a comparison of laboratory and clinical methods for diagnosing pertussis in an outbreak in a facility for the developmentally disabled. | during a pertussis outbreak in a facility for the developmentally disabled, culture- or direct fluorescent-antibody-confirmed cases were identified in 24 residents and 17 staff members; 38 (93%) were culture positive for bordetella pertussis. an enzyme-linked immunosorbent assay (elisa) was used to detect serum igg and iga to the filamentous hemagglutinin and lymphocytosis-promoting factor of b. pertussis. using criteria from elisa values, we identified an additional 83 residents and 28 staff me ... | 1988 | 2893828 |
| the specificity of antisera against bordetella pertussis examined by bacterial agglutination. | the specificity of conventional, adsorbed antisera against agglutinogens 1, 2, and 3 of bordetella pertussis was examined by slide agglutination and by agglutination in microtitre wells. unadsorbed hyperimmune sera showed higher agglutinating activity against autologous or homologous cells than against cells of heterologous serotype. adsorption of sera with heterologous cells increased the serotype specificity considerably. in spite of extensive adsorption, these anti-agglutinogen sera were stil ... | 1987 | 2894108 |
| effects of adenylate cyclase toxin from bordetella pertussis on human neutrophil interactions with coccidioides immitis and staphylococcus aureus. | bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic amp levels and inhibited their oxidative burst, as reflected by luminol-enhanced chemiluminescence and superoxide release. the adenylate cyclase toxin-containing extract blocked neutrophil-mediated inhibition of n-acetylglucosamine incorporation by arthroconidia of coccidioides immitis in a dose-dependent fashion but had no effect on neutrophil phagocytosis of candida glabrata ... | 1988 | 2894360 |
| affinity-based chromatography utilizing genetically engineered proteins. interaction of bordetella pertussis adenylate cyclase with calmodulin. | an engineered calmodulin differs from vertebrate calmodulin in its ability to activate bordetella pertussis adenylate cyclase, and this difference has been utilized as the basis for a new purification protocol for the adenylate cyclase. vu-8 calmodulin, in which 3 glutamic acid residues (residues 82-84) have been substituted with 3 lysine residues, has a 1000-fold lower apparent affinity for the adenylate cyclase, compared to vertebrate calmodulin, and decreased maximal activity. because of the ... | 1988 | 2894377 |
| antimicrobial susceptibilities of bordetella species isolated in a multicenter pertussis surveillance project. | mics for 90% (mic90s) of 75 bordetella pertussis strains for amoxicillin, erythromycin, rifampin, and sulfamethoxazole-trimethoprim were 1, less than or equal to 0.12, 1, and 4 micrograms/ml, respectively. susceptibility rates were all greater than or equal to 93%. only 17% of the strains were susceptible to tetracycline. the mic90s of ciprofloxacin, enoxacin, norfloxacin, ofloxacin, and roxithromycin were less than or equal to 0.06, 0.5, 0.25, 0.12, and 0.5 micrograms/ml, respectively. for b. p ... | 1988 | 2894817 |
| bordetellae and charcoal horse blood agar: inactivation of antibiotics in agar during prolonged incubation for susceptibility testing. | we examined the degree of inactivation of 22 antibiotics caused by prolonged incubation at 36 degrees c of agar plates during agar dilution susceptibility testing of bordetellae. fresh antibiotic-containing plates of charcoal horse blood agar and plates which had been held at 36 degrees c for 2 or 3 days prior to inoculation were inoculated with strains of bordetella pertussis and bordetella parapertussis and incubated for 2 days. then the mics were compared. most antibiotics showed a loss of ac ... | 1988 | 2894923 |
| specific ige antibodies to bordetella pertussis after immunisation in infancy. | | 1988 | 2895248 |
| [physicochemical and immunobiological properties of the dialysate antigen of bordetella pertussis]. | the study of the physicochemical and immunobiological properties of b. pertussis dialysate antigen indicates that the antigen has a complex composition and possesses hemagglutinating and lymphocytosis-promoting activity, which permits further studies with a view to developing diagnostic and prophylactic preparations on the basis of this antigen. | 1987 | 2895557 |
| inhibitors of receptor-mediated endocytosis block the entry of bacillus anthracis adenylate cyclase toxin but not that of bordetella pertussis adenylate cyclase toxin. | bordetella pertussis and bacillus anthracis produce extracytoplasmic adenylate cyclase toxins (ac toxins) with shared features including activation by calmodulin and the ability to enter target cells and catalyze intracellular cyclic amp (camp) production from host atp. the two ac toxins were evaluated for sensitivities to a series of inhibitors of known uptake mechanisms. cytochalasin d, an inhibitor of microfilament function, abrogated the camp response to b. anthracis ac toxin (93%) but not t ... | 1988 | 2895741 |
| immuno-electronmicroscopy of fimbriae-like structures on bordetella pertussis serotype 1.3. | fimbriae-like filaments were demonstrated on the surface of bordetella pertussis, serotype 1.3, by negative staining and electronmicroscopy. immunoelectronmicroscopy with a monoclonal antibody specific for strains possessing agglutinogen 3, and colloidal gold, gave strong labelling of these structures. however, incubation with adsorbed polyclonal anti-agglutinogen 3 serum gave only weak labelling of the distal parts of the filaments and of the bacterial surface. the different binding patterns of ... | 1988 | 2895814 |
| [identification of protein products of the operon for leukocytosis (lymphocytosis)-stimulating factor from bordetella pertussis cloned in escherichia coli]. | the hybrid plasmid prh119 was constructed on the basis of vector plasmid puc19 and shown to carry bordetella pertussis pt operon in the same transcriptional orientation with the lac-promoter of the vector plasmid. expression of pt genes in e. coli cells harbouring prh119 was not registered. weak expression of pt genes was found by immunoscreening of recombinant clones in situ with antiserum against pt when pt genes were put closer to lac-promoter. 0.95 kb salgi fragment was deleted from prh119. ... | 1987 | 2895891 |
| turbinate atrophy in mice caused by bordetella pertussis. | | 1987 | 2895975 |
| the interaction of ca2+ with the calmodulin-sensitive adenylate cyclase from bordetella pertussis. | bordetella pertussis, the etiologic agent of whooping cough, produces a calmodulin-sensitive adenylate cyclase which elevates intracellular camp in a variety of eucaryotic cells. exogenous calmodulin added to the partially purified adenylate cyclase has been shown to inhibit invasion of animal cells by this enzyme (shattuck, r. l., and storm, d. r. (1985) biochemistry 24, 6323-6328). in this study, several properties of the calmodulin-sensitive adenylate cyclase are shown to be influenced by ca2 ... | 1988 | 2896201 |
| release of pertussis toxin and its interaction with outer-membrane antigens. | the absence of subunit s3 in cell-associated pertussis toxin (pt) from a mutant of bordetella pertussis which failed to produce cell-free toxin suggested that this subunit was involved in the release of pt into the culture medium. the addition of methylated beta-cyclodextrin (mcd) to the culture medium caused a small but consistent increase in the release of lipopolysaccharide (lps) by four wild-type strains of b. pertussis. since previous studies have shown that mcd also enhances the levels of ... | 1987 | 2896225 |
| evolutionary relationships in the genus bordetella. | the nucleotide sequence of the pertussis toxin operon of bordetella pertussis, bordetella parapertussis and bordetella bronchiseptica, has shown that the last two species contain many common mutations and are likely to derive from a common ancestor (aricò and rappuoli, 1987). to elucidate further the evolutionary relationships between the bordetella species, we have cloned and sequenced the promoter region and the gene coding for the s1 subunit of pertussis toxin from additional b. pertussis str ... | 1987 | 2896289 |
| adp-ribosyltransferase activity of pertussis toxin and immunomodulation by bordetella pertussis. | pertussis toxin is produced by the causative agent of whooping cough, bordetella pertussis, and is an adenosine diphosphate (adp)-ribosyltransferase capable of covalently modifying and thereby inactivating many eukaryotic g proteins involved in cellular metabolism. the toxin is a principal determinant of virulence in whooping cough and is a primary candidate for an acellular pertussis vaccine, yet it is unclear whether the adp-ribosyltransferase activity is required for both pathogenic and immun ... | 1988 | 2896387 |
| placebo-controlled trial of two acellular pertussis vaccines in sweden--protective efficacy and adverse events. ad hoc group for the study of pertussis vaccines. | 3801 children aged 5-11 months were entered into a blind placebo-controlled trial of pertussis vaccine. 954 were randomised to receive placebo (vaccine solvent), 1419 to receive a two-component vaccine containing formaldehyde detoxified lymphocytosis promoting factor (lpf) and filamentous haemagglutinin, and 1428 to receive an lpf-toxoid vaccine. after 7-13 weeks 3724 infants received a second dose. immediate side-effects were mild. small local reactions occurred more often in the vaccinated inf ... | 1988 | 2896826 |
| specific ige antibodies to bordetella pertussis after immunisation in infancy. | | 1988 | 2896903 |
| cloning and nucleotide sequence analysis of the serotype 2 fimbrial subunit gene of bordetella pertussis. | an oligonucleotide probe complementary to the beginning of the gene encoding the serotype 2(st2) fimbrial subunit of bordetella pertussis was synthesized and a cloned dna fragment hybridizing with the probe identified and sequenced. several lines of evidence indicate that an open reading frame with coding information for a polypeptide of 207 amino acids, including a 26-amino-acid signal sequence, is the st2 gene. the protein deduced from the nucleotide sequence shows good agreement with the nh2- ... | 1987 | 2897065 |
| the calmodulin-sensitive adenylate cyclase of bordetella pertussis: cloning and expression in escherichia coli. | the adenylate cyclase toxin of the prokaryote bordetella pertussis is stimulated by the eukaryotic regulatory protein, calmodulin. a general strategy, using the adenylate-cyclase-calmodulin interaction as a tool, has permitted cloning and expression of the toxin in escherichia coli in the absence of any b. pertussis trans-activating factor. we show that the protein is synthesized in a large precursor form composed of 1706 amino acids. the calmodulin-stimulated catalytic activity resides in the a ... | 1988 | 2897067 |
| morphological changes in rat tracheal mucosa immediately after antigen challenge. | we have investigated the morphological effects of an intratracheal challenge with 50 micrograms ovalbumin (oa) on sensitized rat tracheas, in vivo. female sprague-dawley rats were primed ten days before challenge with a single i.t. injection of 100 micrograms oa plus bordetella pertussis (oa-bp). two additional groups of animals served as controls: primed animals challenged with saline only and non-primed but oa-challenged animals. sacrifices--and subsequent morphological studies--were performed ... | 1987 | 2897214 |
| antimicrobial susceptibility of bordetella pertussis (part i). | in this review of the literature data are collected from the more recent studies on the susceptibility of bordetella pertussis to penicillins, cephalosporins, other beta-lactam antibiotics, aminoglycosides, tetracyclines, erythromycin, josamycin, co-trimoxazole and various other antibiotics. the methods of susceptibility testing of b. pertussis are discussed and suggestions for standardization are made. | 1988 | 2897337 |
| cloning and nucleotide sequence of the aroa gene of bordetella pertussis. | the aroa locus of bordetella pertussis, encoding 5-enolpyruvylshikimate 3-phosphate synthase, has been cloned into escherichia coli by using a cosmid vector. the gene is expressed in e. coli and complemented an e. coli aroa mutant. the nucleotide sequence of the b. pertussis aroa gene was determined and contains an open reading frame encoding 442 amino acids, with a calculated molecular weight for 5-enolpyruvylshikimate 3-phosphate synthase of 46,688. the amino acid sequence derived from the nuc ... | 1988 | 2897356 |
| positive regulation of pertussis toxin expression. | although the genus bordetella contains several closely related species, pertussis toxin (pt) is produced only by phase i bordetella pertussis. in this work we have studied the regulation of expression of the pt operon and investigated why pt is produced by phase i and not by phase iii b. pertussis despite the presence of the pt genes. we have constructed a vector for bordetella species that contains the pt promoter fused to the coding region of the chloramphenicol acetyltransferase (cat) gene, a ... | 1988 | 2897691 |
| immunogenicity of specific bordetella pertussis surface antigens in diphtheria-tetanus-pertussis (dtp) vaccines. | the predominant causative organism of whooping cough in australia is of a serotype which has normally been associated overseas with unvaccinated communities. australian dtp vaccines pass the statutory mouse test for bordetella pertussis potency but this test is now believed to be relatively insensitive to certain factors, especially the major type-specific agglutinogens, which are presumably also important in the human host-parasite relationship. because endemic b. bronchiseptica infections make ... | 1988 | 2897927 |
| specific ige antibodies to bordetella pertussis after immunisation in infancy. | | 1988 | 2898063 |
| genetic analysis of a region of the bordetella pertussis chromosome encoding filamentous hemagglutinin and the pleiotropic regulatory locus vir. | the vir locus of bordetella pertussis apparently encodes a trans-acting positive regulator that is required for the coordinate expression of genes associated with virulence: pertussis toxin, filamentous hemagglutinin (fha), hemolysin, and adenylate cyclase toxin. dna clones of vir and of genes required for the synthesis of some of the factors under vir control were obtained with dna probes from the chromosomal dna surrounding sites of tn5 insertion mutations that inactivated those genes. two vir ... | 1988 | 2898470 |
| specific immunoglobulin a to bordetella pertussis antigens in mucosal secretion for rapid diagnosis of whooping cough. | specific immunoglobulin a (iga) to bordetella pertussis filamentous hemagglutinin (fha) and pertussis toxin (pt) was determined in mucosal secretions by an enzyme-linked immunosorbent assay (elisa). it took 3 to 4 h to complete the elisa. the upper limits of normal values for age were determined in nasopharyngeal (nph) secretions from 23 patients with viral infections and in 10 healthy adults working with pertussis patients or cultures. a significant iga response to fha was found in 38 of 54 (70 ... | 1988 | 2898484 |
| rapid diagnosis of whooping cough using monoclonal antibody. | a counterimmunoelectrophoresis (cie) method for antigen detection using monoclonal antibody was assessed for its ability to aid in the rapid diagnosis of bordetella pertussis in 59 patients. a positive diagnosis from a combination of results from tests of serum and urine was obtained in 51 (87%) of cases. for sera, cie had a sensitivity of 85% and a specificity of 94%; for urine samples the sensitivity was 81% and a specificity of 100%. antigen detection by cie is simple to perform and yields re ... | 1988 | 2898488 |
| receptor analogs and monoclonal antibodies that inhibit adherence of bordetella pertussis to human ciliated respiratory epithelial cells. | the adherence of bordetella pertussis to human respiratory cilia is critical to the pathogenesis of whooping cough. to explore the development of agents that could interrupt adherence, the structure of the receptor on the ciliary surface was investigated. using an in vitro adherence assay to human ciliated epithelial cells, galactose, lactose, and complex carbohydrates containing lactose eliminated adherence when preincubated with the bacteria. 10(-2) m galactose eluted adherent bacteria from ci ... | 1988 | 2899620 |
| differentiation of phase i and variant strains of bordetella pertussis on congo red media. | the addition of congo red, trypan blue or haemin to the growth medium allowed the differentiation of phase-i and variant strains of bordetella pertussis. phase-i strains produced red (cr+), blue or dark brown colonies on a modified cyclodextrin solid medium containing congo red, trypan blue or haemin, respectively, whereas variant (vir- and phase iv) strains grew as pale (cr-) colonies. spontaneous cr- variants were isolated and characterised and had a phenotype like that of vir- or phenotypical ... | 1988 | 2899648 |
| detergent-accelerated hydrolysis of bacterial endotoxins and determination of the anomeric configuration of the glycosyl phosphate present in the "isolated lipid a" fragment of the bordetella pertussis endotoxin. | due to the formation of micelles, severance of the hydrophilic (poly- or oligosaccharide) and hydrophobic ("lipid a") domains of bacterial lipopolysaccharides at ph 3.4 or 4.5 and 100 degrees is slow and sometimes does not proceed at all; partially degraded fragments are usually formed. at ph 3.4 (100 degrees) in aqueous 1% sodium dodecylsulphate (sds), both lipopolysaccharides of the bordetella pertussis endotoxin are cleaved within 20-30 min, but 80% of the glycosidically bound phosphate prese ... | 1988 | 2900066 |
| [isolation and characteristics of the bacteriophage from the vaccine strain tohama phase i]. | some properties of bacteriophage phi t isolated from the vaccine strain bordetella pertussis tohama phase i and propagated in bordetella parapertussis 504 cells are presented. phage phi t belongs to the iv group in accordance with tikhonenko classification. the diameter of head and length of noncontractile tail sheath are 49.5 +/- 0.5 and 145 +/- 7 nm, respectively. diameter of the tail sheath is 3.2 +/- 0.6 nm. molecular mass of the phage dna is 37 +/- 3 kb. population of phi t phage is polymor ... | 1988 | 2900462 |
| extracellular camp formation from host cell atp by bordetella pertussis adenylate cyclase. | the effect of exogenously added adenylate cyclase from bordetella pertussis (strain 114) has been investigated in y-1 mouse adrenal tumor, chinese hamster ovary (cho) and several other cells. a partially purified adenylate cyclase was found not to enter cells but, nevertheless, produced large amounts of camp in the medium. we could show that this resulted from release of atp (and not larger molecules). the atp released by the cells could be (1) directly measured and was replenished after each ch ... | 1988 | 2900655 |
| ige antibody-forming cells in rats infected with nippostrongylus brasiliensis and immunized with antigens. | the distribution of ige antibody-forming cells was examined in rats infected with nippostrongylus brasiliensis (nb) or immunized with nb antigen or with oa. the frequency of antigen-specific ige antibody-forming cells was detected by a passive cutaneous anaphylactic (pca) reaction using cell extract from lymphoid organs. in nb-infected rats, anti-nb and anti-4th stage larvae (l4) ige-forming cells distributed mainly in the mesenteric and the bronchial lymph nodes (ln) near the parasite-harboring ... | 1988 | 2900691 |
| bordetella pertussis adenylate cyclase. penetration into host cells. | exposure of chinese hamster ovary, mouse adrenal cortex tumor (y-1), thp-1 and u-937 cells and human erythrocytes to adenylate-cyclase-containing urea extracts of bordetella pertussis (strain 114) organisms promotes the formation of large concentrations of intracellular camp. accumulation is dependent on dose and temperature, with significant accumulation occurring at 4 degrees c, and is virtually instantaneous, with a doubling at 1 min. there is an absolute ca2+ requirement but external calmodu ... | 1988 | 2900763 |
| new, practical approach to detecting antibody to pertussis toxin for public health and clinical laboratories. | a new, practical method for determining antibody to pertussis toxin (pt) by enzyme-linked immunosorbent assay for public health and clinical laboratories is possible because of recent advances in understanding the pathobiology of bordetella pertussis and the physicochemical properties of pt. the new approach does not require the use of highly purified pt antigen, which is difficult and expensive for most laboratories to obtain. moreover, it employs only reagents that are commercially readily ava ... | 1988 | 2900845 |
| immunoblot analysis of humoral immune responses following infection with bordetella pertussis or immunization with diphtheria-tetanus-pertussis vaccine. | to help develop better diagnostic tests for pertussis, we examined the serologic response to whole-cell proteins of bordetella pertussis after natural infection or vaccination with diphtheria-tetanus-pertussis vaccine. serum specimens collected during a pertussis outbreak investigation and from uninfected persons were used in western blot (immunoblot) analyses to determine the presence of immunoglobulin g (igg) and iga antibodies to specific b. pertussis proteins. igg antibodies to proteins of m ... | 1988 | 2900846 |
| [detection of the gene sequences of the leukocytosis (lymphocytosis)-stimulating factor of bordetella pertussis in bordetella parapertussis and bordetella bronchiseptica by using molecular hybridization]. | the 4.7 kb ecori-fragment of phase i b. pertussis 475 (serovar 1.2.3) chromosome dna carrying the pertussis toxin (pt) operon was cloned on vector plasmid puc19 in escherichia coli. three fragments (1.14 kb kpni-psti, 1.27 kb psti-psti, and 0.96 kb psti-psti) were obtained from the resulting hybrid plasmid, coded prh119, by electrophoretic techniques and used as a combined molecular probe for analysis of the ecori-digested and psti-digested chromosomal dna of b. pertussis strain 475 in phase i, ... | 1988 | 2901175 |
| bordetella pertussis adenylate cyclase. identification of multiple forms of the enzyme by antibodies. | two forms of bordetella pertussis adenylate cyclase of 200 and 47 kda have been purified from dialyzed urea extract of the bacteria to specific activities of 466 and 1685 mumol.min-1.mg-1, respectively. both forms are activated 50-200-fold by calmodulin. the half-maximum concentration required for the activation of the 200-kda catalyst is 5.4.10(-9) m, whereas the one required for activation of the 47-kda catalyst is 1.8.10(-10) m. polyclonal antibodies raised against the 47-kda catalyst specifi ... | 1988 | 2901413 |
| cloning of a novel pilin-like gene from bordetella pertussis: homology to the fim2 gene. | a search for pilin genes in a bordetella pertussis (bp) genomic library has led to the identification of several clones which hybridize to synthetic oligonucleotides with sequences derived from amino acid sequences of bp fimbrial subunits. one of these clones (corresponding to a gene we have named fimx) contains an open reading frame encoding a protein with a molecular weight of about 20 kd and a sequence similar but not identical to the fimbrial subunit fim2 and to other fimbrial protein sequen ... | 1988 | 2902506 |
| serologic diagnosis of whooping cough by enzyme-linked immunosorbent assay. | pernasal swabs were obtained on 3 consecutive days from 146 children referred for hospital admission with suspected whooping cough, and immunoglobulin a and immunoglobulin m antibodies to bordetella pertussis were measured by enzyme-linked immunosorbent assay. the clinical features in 113 of the children were considered consistent with the diagnosis. sixty-four cases were confirmed by serology, which showed a greater sensitivity (57% vs. 35%) than pernasal swab culture with no loss of specificit ... | 1988 | 2902556 |
| subunit s1 of pertussis toxin: mapping of the regions essential for adp-ribosyltransferase activity. | the toxicity of pertussis toxin is mediated by the adp-ribosyltransferase activity of subunit s1. to understand the structure-function relationship of subunit s1 and guide the construction of nontoxic molecules suitable for vaccines, we constructed and expressed in escherichia coli a series of amino-terminal and carboxyl-terminal deletion mutants as well as a number of molecules containing amino acid substitutions. the shortest peptide still retaining enzymatic activity contains amino acids 2-17 ... | 1988 | 2902632 |
| inhibition of activated nonresponder c3h/hej lymphocytes by lipopolysaccharide endotoxin. | the b lymphocytes and macrophages of lipopolysaccharide (lps) nonresponder c3h/hej mice were found to respond to certain r types of lps endotoxin in a fashion resembling that ordinarily seen with the cells from normal responder mice. dna synthesis, polyclonal antibody synthesis, and interleukin-1 activity were stimulated by bordetella pertussis lps and salmonella minnesota r595 lps, although to a lesser extent than with responder cells. mitogenesis stimulated by both lpss was inhibited by polymy ... | 1988 | 2903122 |
| comparison of type 2 and type 6 fimbriae of bordetella pertussis by using agglutinating monoclonal antibodies. | two types of fimbriae have been identified on the pathogenic gram-negative organism bordetella pertussis. monoclonal antibodies to these fimbriae were produced to better understand the role of fimbriae as serotype-specific agglutinogens and to investigate the antigenic relationship between these fimbriae. three monoclonal antibodies were identified that specifically agglutinated b. pertussis cells containing the u.s. reference factor 2 agglutinogen, and six monoclonal antibodies were produced th ... | 1988 | 2903125 |
| identification of a 69-kilodalton nonfimbrial protein as an agglutinogen of bordetella pertussis. | cells of bordetella pertussis bp353, a nonfimbriated eldering serotype 1.3 strain, were used as an immunogen to produce three monoclonal antibodies, bpe3, bpd8, and bpe8, that agglutinated the immunizing cells, as well as certain other nonfimbriated and fimbriated serotype 3-containing b. pertussis strains. the antibodies did not agglutinate serotype 1 or nontypable b. pertussis cells. these monoclonal antibodies specifically detected a 69-kilodalton (kda) band on western blots (immunoblots) con ... | 1988 | 2903126 |
| two trans-acting regulatory genes (vir and mod) control antigenic modulation in bordetella pertussis. | expression of virulence factors by bordetella pertussis is altered by environmental signals (antigenic modulation) and is dependent on an activator encoded by a gene called vir. we have used tnphoa (tn5 is50l::phoa) gene fusions to define two sets of genes whose expression is either activated (vag loci) or repressed (vrg loci) by modulation signals. both groups of genes appear to be regulated by the vir gene product in that, in the absence of modulators, null mutations in vir lead to the repress ... | 1988 | 2903140 |
| monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for detection of bordetella pertussis filamentous hemagglutinin. | hybrid cell lines producing monoclonal antibodies against bordetella pertussis filamentous hemagglutinin (fha) were established. the specificity of the antibodies was ascertained by enzyme-linked immunosorbent assay (elisa), sandwich elisa, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroblotting. the monoclonal antibody-based sandwich elisa was developed for detection of b. pertussis fha. the assay had a detection limit of b. pertussis fha in concentrations rang ... | 1988 | 2903174 |
| effects of transport temperature and medium on recovery of bordetella pertussis from nasopharyngeal swabs. | we compared relative recoveries of bordetella pertussis from simulated nasopharyngeal (np) specimens incubated in three separate transport media at different temperatures. transport media included one-half-strength regan-lowe (rl.5), regan-lowe with one-half-strength agar (rl.5a), and buffered charcoal-yeast extract agar supplemented with alpha-ketoglutarate, lincomycin, and anisomycin (bcye alpha la). for each transport medium, recovery of b. pertussis was least efficient after storage at 25 de ... | 1988 | 2903177 |
| evaluation of serologic assays for diagnosis of whooping cough. | an enzyme-linked immunosorbent assay (elisa) for the immunoglobulin g (igg), igm, and iga response to bordetella pertussis filamentous hemagglutinin (fha) and pertussis toxin (pt) and a neutralization test (nt) in a microplate tissue culture assay for neutralizing antibodies to pt were evaluated in paired sera from 90 patients with culture-confirmed pertussis. eighty patients were children (age, less than 15 years), and 6 of 80 children had been immunized with three doses of diphtheria-tetanus-p ... | 1988 | 2903178 |
| naturally crystalline porin in the outer membrane of bordetella pertussis. | the gram-negative bacterium bordetella pertussis is the agent responsible for whooping-cough, and much interest has focused on the functions, structures and immunological properties of the molecules exposed at its outer surface. we have found by electron microscopy that cells of two strains of b. pertussis are covered with a crystalline surface lattice. this lattice is not an extrinsic layer of high molecular weight glycoproteins, such as occur on many other bacteria, but is a natural crystal of ... | 1988 | 2903251 |
| surface proteins of bordetella pertussis. | bordetella pertussis cells express multiple virulence-associated surface proteins, including adenylate cyclase, agglutinogens 2 and 3, filamentous hemagglutinin, pertussis toxin, and outer-membrane protein (omp) 30/32 and omp91. surface proteins that are not virulence-associated include three peptidoglycan-associated omps of apparent molecular weights 40,000, 25,000, and 18,000. omp40 is an anion-selective porin and is the most abundant surface protein of virulent and avirulent cells. three inde ... | 1988 | 2903539 |
| synthetic peptides based on the calmodulin-binding domain of myosin light chain kinase inhibit activation of other calmodulin-dependent enzymes. | nanomolar concentrations of synthetic peptides corresponding to the calmodulin-binding domain of skeletal muscle myosin light chain kinase were found to inhibit calmodulin activation of seven well-characterized calmodulin-dependent enzymes: brain 61 kda cyclic nucleotide phosphodiesterase, brain adenylate cyclase, bordetella pertussis adenylate cyclase, red blood cell membrane ca++-pump atpase, brain calmodulin-dependent protein phosphatase (calcineurin), skeletal muscle phosphorylase b kinase, ... | 1988 | 2903735 |
| all species of the genus bordetella contain genes for pertussis toxin of bordetella pertussis. | a molecular probe for the pt-operon of b. pertussis hybridized with 4.7 kb ecori-fragments of chromosomal dnas of b. pertussis strain 475 phase i, phase iv, b. parapertussis strains 504 and 17903, b. bronchiseptica strain 214, b. parapertussis strain 17903-convertant of b. pertussis phage 134 but not with phage 134 dna under stringent conditions of dna-dna hybridization. this fact indicates the presence of pt-genes in all bordetella species. since there is no production of pt in b. parapertussis ... | 1988 | 2904197 |
| [effect of histamine on the rosette-forming capacity of t-lymphocytes in immunization with adpt vaccine and its components]. | the results obtained in the study of the influence of histamine on the capacity of t-lymphocytes of guinea pigs immunized with dpt-vaccine and its components for spontaneous rosette formation are presented. histamine at a concentration of 10(-3) m has been found to inhibit the capacity of blood and splenic lymphocytes of guinea pigs immunized with adsorbed dpt vaccine for spontaneous rosette formation. the inhibitory effect is more pronounced after the immunization of the animals with adsorbed d ... | 1988 | 2904199 |