| bacteriophages of methanotrophs isolated from fish. | bacteriophages of methanotrophic bacteria were isolated from 67 fish. only two phages isolated from two fish species specifically lysed methylocystis sp. and flavobacterium gasotypicum. the phages lysing these species were designated 63-f and cmf-1-f, respectively. the isolated phages differed greatly in the fine structure of the virion, plaque morphology, spectrum of lytic action, serological properties, and uv sensitivity. at the same time, they had identical one-step growth characteristics: t ... | 1983 | 6416169 |
| microbial degradation of synthetic organochlorine compounds. | | 1983 | 6416886 |
| chemical wastes and their biodegradation--an overview. | | 1983 | 6416887 |
| [endo-beta-galactosidase from flavobacterium keratolyticus and escherichia freundii]. | | 1984 | 6431498 |
| in vitro activity of cefoperazone against nonfermenters and aeromonas hydrophila. | the in vitro activity of cefoperazone against 380 strains (33 species and unnamed groups) of nonferenters and 20 strains of aeromonas hydrophila was studied by a microdilution method of determining minimal inhibitory concentrations. for comparison, the activities of ampicillin, ticarcillin, and cefamandole were simultaneously studied. cefoperazone was the most active drug against pseudomonas sp., achromobacter xylosoxidans, flavobacterium meningosepticum. flavobacterium sp. (group iib), group iv ... | 1980 | 6448581 |
| [studies on fermented corn flour poisoning--a new species of food poisoning bacteria: flavobacterium farinofermentans n. sp. (author's transl)]. | | 1980 | 6448712 |
| degradative removal of heparan sulfate from the surface of an ascites hepatoma, ah 66, by heparitinase. | heparitinase [ec 4.2.2.8, heparitin sulfate lyase] was prepared from an extract of cultured cells of flavobacterium heparinum. purification of the enzyme was achieved by repeating the hydroxyapatite column chromatography. the enzyme was used to degrade heparan sulfate occurring on the surfaces of ascites hepatoma cells, ah 66. from the supernatant of the enzyme-treated cells, breakdown products from heparan sulfate could be detected by paper chromatography. the heparitinase was found to be more ... | 1981 | 6452448 |
| bacterial survival in a dilute environment. | bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. this approach permitted separation of the lake isolates into two categories. members of the tribe klebsielleae were shown to have a prolonged survival rate of 40% or better after 24 h, whereas nonsurvivors were not viable for much longer than 24 ... | 1981 | 6454390 |
| involvement of cell surface heparin sulfate in the binding of lipoprotein lipase to cultured bovine endothelial cells. | it has been postulated that lipoprotein lipase, an enzyme important in the uptake of fatty acids into tissues, is bound to the vascular endothelial cell surface and that this binding occurs through attachment to heparinlike glycosaminoglycans. furthermore, it is thought that heparin releases the enzyme from its attachment to the endothelium into the circulation. these hypotheses have never been tested directly in cell systems in vitro. in the present study we have directly evaluated the interact ... | 1981 | 6457061 |
| [determination of g-c mole percentage in dna of flavobacterium farinofermentans n.sp. (author's transl)]. | | 1981 | 6458412 |
| heparin-like molecules as regulators of atherogenesis. | bovine aortic endothelial cells release a heparin-like substance in the presence of 0.4% fetal calf serum. this substance inhibited the growth of smooth muscle cells in vitro by about 70%. substitution of platelet-poor plasma for serum resulted in minimal liberation of inhibitory activity from the cells unless at least 10-fold higher concentrations of platelet-poor plasma were utilized. this suggested that a platelet product was involved in the release process. therefore, we examined the ability ... | 1984 | 6473095 |
| an immobilized microbial heparinase for blood deheparinization. | a new medical application of an immobilized microbial enzyme is described. extracorporeal devices require systemic heparin administration to prevent thrombus formation; however, the use of heparin often leads to serious hemorrhagic complications. heparinase isolated from flavobacterium has been immobilized and used in a fluidized bed reactor to eliminate heparin from blood passing through an extracorporeal circuit both in vitro and in vivo. this paper discusses the stepwise development of this h ... | 1984 | 6476820 |
| evaluation of the rapid nft system for identification of gram-negative, nonfermenting rods. | this study evaluated the ability of the rapid nft system (api system sa, montalieu-vercieu, france) to accurately identify 262 clinically isolated, gram-negative, nonfermentative rods without additional tests. identifications were classified as correct; low discrimination, with a spectrum of two or more possibilities (additional tests necessary for accurate identification); and incorrect. correct identification rates were analyzed in two categories: (i) correct to species or biotype for all orga ... | 1984 | 6490857 |
| empedopeptin (bmy-28117), a new depsipeptide antibiotic. i. production, isolation and properties. | empedopeptin is a new antibiotic produced by empedobacter haloabium nov. sp. (atcc 31962). it is a water-soluble depsipeptide antibiotic containing eight amino acid residues and a c14-fatty acid moiety in the molecule. although structurally unrelated, empedopeptin and vancomycin have similar antimicrobial spectra against aerobic and anaerobic gram-positive bacteria including antibiotic-resistant strains. empedopeptin is highly active in vivo in mice against systemic infections of staphylococcus ... | 1984 | 6501108 |
| isolation and characterization of a new cytophaga species implicated in a work-related lung disease. | a yellow-pigmented, gram-negative, gliding bacterium isolated from an industrial water spray air humidification system was implicated as a causative agent in several occurrences of lung disease with hypersensitivity pneumonitis-like symptoms. the bacterium, designated wf-164, lacked microcysts or fruiting bodies and had a dna base composition of 34.8 mol% of guanine plus cytosine. gliding, flexing, nonflagellated cells measuring 0.3 by 3.5 to 8.9 micron were observed by using light and electron ... | 1984 | 6508308 |
| flavobacterium heparinum 2-o-sulphatase for 2-o-sulphato-delta 4,5-glycuronate-terminated oligosaccharides from heparin. | the glycosulphatase which hydrolyses the 2-o-sulphate of the disaccharide, 4-deoxy-2-o-sulphato-alpha-l-threohex-4-enopyranosyl uronic acid-(1----4)-2-deoxy-2-sulphamido-6-o-sulphato-d-glucose (delta ua-2s----glcns-6s), has been isolated from the soluble fraction of disrupted flavobacterium heparinum. the activity was purified 3300-fold by chromatography on cm-sepharose cl-6b, hydroxyapatite, taurine-sepharose cl-4b and blue-sepharose cl-6b. from sodium dodecylsulphate/polyacrylamide gel electro ... | 1984 | 6510419 |
| flavobacterium meningitis. | | 1984 | 6519786 |
| [microbiological studies of ground water polluted with hydrocarbons. 1. quantitative and qualitative distribution of bacterial populations]. | the groundwater, polluted with hydrocarbons, showed large cell numbers during one year of examination: 10(3)-10(4) cfu/ml grown on n-agar after 5 days at 20 degrees c, 10(4)-10(5) cfu/ml grown on water-agar after 8 weeks at 20 degrees c and total cell numbers of 10(5)-10(7)/ml. the bacteria, which were isolated from n-agar, were chiefly pseudomonas, flavobacterium, acinetobacter, and other gramnegative species, arthrobacter and bacillus as members of grampositive bacteria. furthermore the follow ... | 1984 | 6524146 |
| sequence variation in heparin octasaccharides with high affinity for antithrombin iii. | we have isolated from nitrous acid cleavage products of heparin two major octasaccharide fragments which bind with high affinity to human antithrombin. octasaccharide s, with the predominant structure iduronic acid----n-acetylglucosamine 6-o-sulfate----glucuronic acid-----n-sulfated glucosamine 3,6-di-o-sulfate----iduronic acid 2-o-sulfate----n-sulfated glucosamine 6-o-sulfate----iduronic acid 2-o-sulfate----anhydromannitol 6-o-sulfate, is sensitive to cleavage by flavobacterium heparinase as we ... | 1984 | 6525337 |
| characterization of polysaccharides of the eggs and adults of hymenolepis diminuta. | polysaccharides and other complex carbohydrates were released by proteolysis of the chloroform-methanol insoluble residue of 10 day-old worms and eggs of hymenolepis diminuta. gas-liquid chromatographic analysis of alditol acetate derivatives of monosaccharides released from the polysaccharides by hydrolysis revealed that in the 10 day-old worm, glucose was the most abundant sugar, followed by galactose, glucosamine, galactosamine, fucose and possibly rhamnose. mannose was least abundant and xyl ... | 1984 | 6537986 |
| bacterial production of 7-formamidocephalosporins. isolation and structure determination. | two new 7-formamidocephalosporins have been isolated as their acetyl derivatives (sq 28,516 and sq 28,517) from fermentations of a flavobacterium sp. sc 12,154. structure 1 was deduced for sq 28,516 from its spectroscopic properties while structure 2 was proposed for sq 28,517. sq 28,516 exhibits weak antibacterial activity. | 1984 | 6547947 |
| microbial degradation of validamycin a by flavobacterium saccharophilum. enzymatic cleavage of c-n linkage in validoxylamine a. | the enzymatic cleavage of c-n linkage in the degradation of validamycin a by flavobacterium saccharophilum was examined using n-p-nitrophenyl derivatives of validamine and valienamine as synthetic model substrates for validoxylamine a. incubation of n-p-nitrophenylvalidamine with the membrane fraction from the organism led to formation of n-p-nitrophenyl-3-ketovalidamine, and succeeding cleavage of c-n linkage. as the products of the cleavage step, one was identified as p-nitroaniline and anothe ... | 1984 | 6548220 |
| chitinovorins a, b and c, novel beta-lactam antibiotics of bacterial origin. | | 1984 | 6549002 |
| [an in vitro study of susceptibility of flavobacterium meningosepticum to antibiotics]. | the present study concern in vitro observation on the susceptibility to 20 antibacterial agents of the strains of flavobacterium meningosepticum from clinical specimens at juntendo university hospital during the 1 year period of 1981. the tests for susceptibility of the 144 strains to the drugs were all performed by the serial 2-fold agar plate dilution method on mueller hinton agar (difco), standardized by the japan society of chemotherapy using the microplanter apparatus with one loop of appro ... | 1983 | 6551461 |
| flavobacterium meningosepticum. | lavobacterium meningosepticum is an opportunistic pathogen of low virulence found in the hospital environment in water-containing equipment. of primary importance is its role in outbreaks of neonatal meningitis which tends to be severe with a high mortality rate and serious sequelae. changing all equipment concerned with humidifying or administering gases every 24 hours can help prevent these outbreaks in neonatal nurseries. treatment is difficult because of the resistance of f. meningosepticum ... | 1984 | 6563023 |
| direct dilution sampling, quantitation, and microbial assessment of open-system ventilation circuits in intensive care units. | in a systematic approach, 37 duplicate samples of open system circuits (bennett ma-1 ventilators) of patients in medical and surgical intensive care units were processed by direct and serial (apha guidelines) dilutions. the paired difference test on 15 of the in-use circuitry solution samples indicated no difference between the direct and serial dilution methods (p less than 0.001). seventy-seven additional respiratory therapy circuitry samples from similar intensive care patients were analyzed ... | 1983 | 6575015 |
| syntheses of optically active amino acids by the combination of chemical methods and microbial techniques. | the newly developed processes combining the advantages of chemical methods and microbial techniques brought about optically active amino acids (d- or l-) from racemic compounds. in all cases, the other isomer of substrates (l- or d-) that cannot be catalyzed by hydrolyzing enzymes were easily racemized under reaction conditions. then, optically active amino acids were produced quantitatively from the racemic compounds. | 1983 | 6584072 |
| birth of a unique enzyme from an alternative reading frame of the preexisted, internally repetitious coding sequence. | the mechanism of gene duplication as the means to acquire new genes with previously nonexistent functions is inherently self limiting in that the function possessed by a new protein, in reality, is but a mere variation of the preexisted theme. as the source of a truly unique protein, i suggest an unused open reading frame of the existing coding sequence. only those coding sequences that started from oligomeric repeats are likely to retain alternative long open reading frames. analysis of the pub ... | 1984 | 6585807 |
| segmental homology and internal repetitiousness identified in putative nucleic acid polymerase and human hepatitis b surface antigen of human hepatitis b virus. | in a previous paper, it was argued that only those coding sequences descended from oligomeric repeats (the number of bases in the oligomeric unit not being a multiple of 3) can retain sufficiently long alternative open reading frames, and that such alternative open reading frames serve as the reservoir for the sudden generation of new polypeptide chains with novel functions. it was suggested that plasmid-encoded 6-amino hexanoic acid linear oligomer hydrolase that suddenly endowed flavobacterium ... | 1984 | 6587393 |
| use of a synthetic oligonucleotide to identify a chromosomal gene for chloramphenicol acetyltransferase in a plasmid-bearing flavobacterium. | a micro-organism previously designated flavobacterium sp. cb60 is resistant to chloramphenicol as a consequence of antibiotic acetylation by the enzyme chloramphenicol acetyltransferase and subsequent degradation of the acetylated product by co-metabolism. although a 15.6 kb plasmid (pcb60) was demonstrated in this flavobacterium strain, it did not appear to play a role in chloramphenicol acetylation. dna hybridization was used to identify a fragment of dna presumptively carrying the cat gene. t ... | 1984 | 6596405 |
| 3-dimensional susceptibility testing of beta-lactam antibiotics. | a technique for demonstrating the substrate specificities of bacterial beta-lactamases is presented. enzymatic degradation of beta-lactam antibiotics can be detected by cutting a wall of inoculum into the agar 3 mm from an antibiotic disc. inactivation of the drug as it diffuses through the wall of inoculum results in distortion of the resulting inhibition zone. this procedure can be applied to the stokes, bauer-kirby, international collaborative study (ics) and calibrated dichotomous sensitivit ... | 1984 | 6607917 |
| trimethoprim sulphamethoxazole in neonatal flavobacterium meningosepticum infection. | during an outbreak of flavobacterium meningosepticum septicaemia in a neonatal intensive care unit 9 infants were treated with intravenous trimethoprim sulphamethoxazole. bacteriological cure was achieved in 8 patients; one infant died of massive intraventricular haemorrhage on the first day of treatment. apart from prolonged persistence of pre-existing thrombocytopenia there was no evidence of side effects. trimethoprim sulphamethoxazole should be considered in the treatment of neonatal f menin ... | 1984 | 6611137 |
| flavobacterial meningitis in an infant. | | 1983 | 6618568 |
| meningitis caused by flavobacterium meningosepticum after transsphenoidal hypophysectomy with recovery. | adult meningitis caused by flavobacterium meningosepticum is rare. five cases have been reported in the literature. the case reported herein developed in a woman after a transsphenoidal hypophysectomy. the isolate was resistant to erythromycin. treatment with oral rifampicin combined with intravenous chloramphenicol and cefoperazone resulted in complete recovery. | 1983 | 6623338 |
| successful treatment of neonatal flavobacterium meningosepticum infection. | an 8-day-old, 2.48-kg, 35-week gestation infant developed neonatal sepsis and meningitis due to flavobacterium meningosepticum serotype f. treatment with a new antibiotic, azlocillin, in combination with chloramphenicol, led to complete recovery. | 1983 | 6628455 |
| heparinase: in vivo activity and immunogenicity in rabbits. | anticoagulation with heparin is required during extracorporeal circulation for hemodialysis and cardiopulmonary bypass as well as during vascular surgery. reversal of anticoagulation with protamine may be associated with hypotension and rebound anticoagulation and requires stoichiometric doses. heparinase from flavobacterium heparinum catalytically degrades heparin and reverses its anticoagulant effect. heparin was administered to new zealand white rabbits and plasma levels were assayed with the ... | 1983 | 6631173 |
| rifampin therapy for brucellosis, flavobacterium meningitis, and cutaneous leishmaniasis. | rifampin has a broad antibacterial spectrum. at high concentrations it also is active in vitro against protozoa, i.e., different species of leishmania. rifampin has been used against bacterial and occasionally protozoal infections. addition of rifampin to a tetracycline regimen was found to reduce the number of relapses in patients with acute and chronic brucellosis. in a few cases of meningitis due to flavobacterium meningosepticum, which failed to respond to other drugs, patients were treated ... | 1983 | 6635449 |
| [biosynthesis of phenylalanine and tyrosine in flavobacteria]. | the enzymes of the terminal steps of phenylalanine and tyrosine biosynthesis, chorismate mutase, prephenate dehydratase, arogenate dehydratase, prephenate dehydrogenase and arogenate dehydrogenase, were studied in 11 different species of the genus flavobacteria. a comparison of the specific activities, cofactor specificity and regulation of the enzymes, allows a differentiation within the flavobacteria. all strains studied utilize both arogenate and p-hydroxyphenylpyruvate as an intermediate in ... | 1983 | 6642432 |
| evolutionary adaptation of plasmid-encoded enzymes for degrading nylon oligomers. | flavobacterium sp. ki72 metabolizes 6-aminohexanoic acid cyclic dimer, a by-product of nylon manufacture, through two newly evolved enzymes, 6-aminohexanoic acid cyclic dimer hydrolase (ei) and 6-aminohexanoic acid linear oligomer hydrolase (eii). these enzymes are active towards man-made compounds, the cyclic dimer and linear oligomers of 6-aminohexanoic acid respectively, but not towards any of the natural amide bonds tested. the structural genes of ei (nyla) and eii (nylb) are encoded on poad ... | 1983 | 6646204 |
| satellite growth of legionella pneumophila with an environmental isolate of flavobacterium breve. | legionella pneumophila serogroup 1 was observed to satellite around colonies of flavobacterium breve on an l-cysteine-deficient medium which did not support growth of legionellae. both isolates were recovered from the hot water tanks of hospitals. ferric ppi stimulated satellite growth between 0.01 and 0.1%. | 1983 | 6660882 |
| meningitis caused by a flavobacterium-like organism (cdciie strain) | | 1983 | 6663088 |
| isolation and identification of ropy bacteria in raw milk. | approximately 4.2% of 4,000 maryland-virginia raw milk tanker samples developed ropiness when incubated at 10 degrees c. of the 56 bacterial isolates 30 were identified by species. klebsiella oxytoca and pseudomonas aeruginosa were isolated most frequently. other ropy isolates were identified as pseudomonas spp., chromobacterium, flavobacterium multivorum, presumptive yersinia pestis, enterobacter agglomerans, klebsiella pneumoniae, and pasteurella-actinobacter spp. six of the klebsiella oxytoca ... | 1983 | 6685140 |
| bacterial interference with coliform colony sheen production on membrane filters. | the membrane filter (mf) method for detection and enumeration of coliform bacteria in drinking water requires that the coliforms both grow and produce a green metallic sheen when the filter is incubated on modified endo medium at 35 degrees c for 22 h. large numbers of noncoliform bacteria, which are enumerated by the standard plate count (spc) technique, can interfere with the detection of coliforms on mf. this paper presents quantitative evidence from laboratory experiments on the interference ... | 1984 | 6696423 |
| structural studies on heparin. tetrasaccharides obtained by heparinase degradation. | three tetrasaccharides representing major structural sequences of heparin were isolated in good yield and characterized after degradation of heparin by purified flavobacterial heparinase. n-desulfation was necessary to achieve good separation of these closely related compounds from each other. one of the tetrasaccharides was shown to be derived from the fully sulfated repeating segments; to contain l-iduronic acid and six sulfate groups, and have the structure delta 4,5- hexpa -(2-so4)-(1----4)- ... | 1984 | 6713443 |
| flavobacterium multivorum septicemia in a hemodialyzed patient. | a case of flavobacterium multivorum septicemia in a hemodialyzed patient is reported. two blood cultures were positive at 48 h, and the patient became afebrile only after antimicrobial therapy. the origin of the septicemia could not be determined. | 1984 | 6715525 |
| heterotrophic nitrification among denitrifiers. | twelve denitrifying bacteria representing six genera were tested for an ability to nitrify pyruvic oxime heterotrophically. six of these bacteria exhibited appreciable nitrification activity, yielding as much as 5.8 mm nitrite and little or no nitrate when grown in a mineral salts medium containing 7 mm pyruvic oxime and 0.05% yeast extract. of the six active bacteria, four (pseudomonas denitrificans, pseudomonas aeruginosa, and two strains of pseudomonas fluorescens) could grow on yeast extract ... | 1984 | 6721486 |
| bactericidal activity of ethanol against glucose nonfermentative gram-negative bacilli. | the bactericidal effect of ethanol on glucose nonfermentative gram-negative bacilli (nonfermentative bacilli) and other species of micro-organisms was studied with emphasis on the former. at 20 degrees c, 10 to 20% v/v ethanol took 1 h or more to kill thirteen strains of nonfermentative bacilli while 40 to 99.5% concentrations produced a bactericidal effect within 1 min of exposure. eleven strains of glucose fermentative organisms showed a similar tendency to that noted with nonfermentative baci ... | 1984 | 6727697 |
| acceleration of thrombin-antithrombin complex formation in rat hindquarters via heparinlike molecules bound to the endothelium. | we have examined the role of heparinlike molecules in the regulation of coagulation by perfusing rat hindquarters with purified human thrombin and with its plasma inhibitor, antithrombin. our data indicate that contact of the hemostatic components with the endothelium enhances the rate of thrombin-antithrombin complex formation by as much as 19-fold over the uncatalyzed rate of enzyme-inhibitor interaction. heparinlike molecules are responsible for the antithrombin accelerating activity. the amo ... | 1984 | 6746897 |
| nosocomial respiratory infection: the essential role of the laboratory in control efforts. | | 1982 | 6749391 |
| proline-specific dipeptidyl aminopeptidase from flavobacterium meningosepticum. | a proline-specific dipeptidyl aminopeptidase was highly purified from cell-free extract of flavobacterium meningosepticum by a series of column chromatographies on deae-sephadex a-50, sephadex g-150, hydroxyapatite, and a second gel filtration on sephadex g-150. the enzyme was most active at ph 7.4-7.8 for both gly-pro-beta-naphthylamide (gly-pro-2-nnap) and gly-pro-p-nitroanilide (gly-pro-pna) and was stable between ph 7 and 9.5. the enzyme was markedly inhibited by diisopropylphosphofluoridate ... | 1982 | 6749829 |
| the n/f and oxi/ferm systems for identification of oxidative-fermentative gram-negative rods: a comparative study. | two commercial systems, the flow n/f system and the roche oxi/ferm tube for identification of oxidative-fermentative gram-negative rods were compared. both systems were easy to use, but several reactions in both kits were sometimes difficult to interpret. of 53 reference strains, 70 per cent were identified correctly by the n/f system, whereas the oxi/ferm tube identified 43 per cent. incorrect identities were obtained in 9 per cent and 26 per cent of cases by the n/f and oxi/ferm kits respectiv ... | 1982 | 6756031 |
| the populations, characterization and activity of suspended bacteria in the welsh river dee. | | 1982 | 6757240 |
| endophthalmitis: current approaches. | seventy patients referred with a diagnosis of endophthalmitis underwent anterior chamber and vitreous taps with intracameral antibiotic injections. fifty-four eyes were culture positive, 34 (63%) after previous intraocular surgery, 12 (22%) had sustained penetrating trauma, and 8 (15%) resulted from a metastatic infection. of 61 total isolates, 48 (79%) were gram positive, 9 (15%) were gram negative, and 5 (8%) were fungi. visual recovery after surgery was related to the relative virulence of th ... | 1982 | 6757823 |
| accuracy of a rapid carbohydrate oxidation microtube method for identification of nonfermentative gram-negative bacilli. | a rapid carbohydrate oxidation microtube system (carr microbiologicals, wichita, kans.), designed for detecting the saccharolytic activity of gram-negative, nonfermenting bacilli, was evaluated and compared with the conventional oxidation-fermentation method. the oxidation of glucose, maltose, lactose, and xylose was tested with 430 strains of pseudomonas, acinetobacter, achromobacter, alcaligenes, moraxella, flavobacterium, and bordetella species. more than 95% of the isolates tested gave corre ... | 1982 | 6764775 |
| proline-specific endopeptidase from flavobacterium. purification and properties. | | 1980 | 6768725 |
| l-lysine: 2-oxoglutarate 6-aminotransferase. subunit structure composed of non-identical polypeptides and pyridoxal 5'-phosphate-binding subunit. | l-lysine:2-oxoglutarate 6-aminotransferase from flavobacterium lutescence (= achromobacter liquidum) has been shown to be composed of one each of four non-identical subunits, a, b1, b2, and c. the subunits were isolated by gel filtration, and deae-cellulose chromatography in the presence of 8 m urea. their molecular weights were determined by ultracentrifugation, gel electrophoresis and gel filtration: subunit a 24,000; b1 28,000; b2 28,000; c 45,000. these subunits were all different in amino a ... | 1980 | 6771255 |
| l-lysine epsilon-aminotransferase involved in cephamycin c synthesis in streptomyces lactamdurans. | in streptomyces lactamdurans, the precursor of the alpha-aminoadipoyl side-chain of cephamycin c is l-lysine. in this regard, streptomycetes differ strikingly from the fungi, which produce alpha-aminoadipic acid during the synthesis, rather than the breakdown, of l-lysine. studies using a cell-free system showed that an aminoadipic acid. the product of this reaction was trapped and subsequently purified by ion-exchange chromatography. thin-layer chromatography, spectrophotometry, and amino acid ... | 1980 | 6772093 |
| a novel purification procedure of l-lysine 6-aminotransferase from flavobacterium lutescence. | a new method for the purification of l-lysine 6-aminotransferase (l-lysine: 2-oxoglutarate 6-aminotransferase, ec 2.6.1.36) was devised, in which affinity chromatography with l-lysylacetamidododecyl-sepharose 6b, the most effective affinity adsorbent, was substituted for the heat treatment. the yield of the enzyme with the present procedure was approx. twice as high as that with the previous procedure (soda, k. and misono, h. (1968) biochemistry 7, 4110-4119). the enzyme purified by this method ... | 1980 | 6772228 |
| bacteriophages of methanotrophic bacteria. | bacteriophages of methanotrophic bacteria have been found in 16 out of 88 studied samples (underground waters, pond water, soil, gas and oil installation waters, fermentor cultural fluids, bacterial paste, and rumen of cattle) taken in different geographic zones of the soviet union. altogether, 23 phage strains were isolated: 10 strains that specifically lysed only methylosinus sporium strains, 2 strains that each lysed 1 of 5 methylosinus trichosporium strains studied, and 11 strains that lysed ... | 1980 | 6774962 |
| [on the occurrence of staphylococci and pseudomonas in swimming-pool water (author's transl)]. | 1. analyses made at a bath with an additional ozone processing stage showed that the bathers caused a marked bacterial contaminatin on busy days and that this contamination did not originate from the purification plant. 2. this finding and numerous individual analyses of water from various swimming pools yielded a spectrum of 14 micro-organisms, 4 genera being of frequent incidence: bacillus, staphylococcus, pseudomonas and flavobacterium. in general, bacterial contamination exists not only in t ... | 1980 | 6775458 |
| cellular location of enzymes involved in chondroitin sulfate breakdown by bacteroides thetaiotaomicron. | bacteroides thetaiotaomicron, a gram-negative anaerobe found in human colons, could utilize chondroitin sulfate, a tissue mucopolysaccharide, as its sole source of carbohydrate. the enzymes responsible for the breakdown of chondroitin sulfate by b. thetaiotaomicron were similar to those produced by proteus vulgaris and flavobacterium heparinum and included a lyase (ec 4.2.2.4), which degraded chondroitin sulfate into sulfated disaccharides, sulfatases (ec 3.1.6.4), which removed the sulfate resi ... | 1980 | 6782076 |
| [bacteremia due to flavobacterium meningosepticum. clinical and bacteriological aspects of patients (author's transl)]. | | 1980 | 6782178 |
| isolation and characterization of a keratan sulfate-degrading endo-beta-galactosidase from flavobacterium keratolyticus. | | 1981 | 6783650 |
| effects of activated carbon and bacteriostatic filters on microbiological quality of drinking water. | three activated carbon filters for point-of-use water treatment were tested in laboratory and field studies for chemical removal and microbiological effects on water. all removed free available chlorine in municipally treated water to below the limit of detection, but removed only about 50 to 70% of the total available chlorine and 4 to 33% of the total organic carbon. standard plate count bacteria in the effluent increased steadily with time for 3 weeks and remained elevated over the 8-week per ... | 1981 | 6784673 |
| proteoglycans and chondroitin sulfates from human multiple chondroma (enchondromatosis). | 1. this paper reports the structural analysis of proteoglycans and mucopolysaccharides extracted from a human multiple enchondroma (enchondromatosis), a benign cartilage tumor, where growth, but no calcification takes place. the tumors were located inside the phalanges of both hands of a 22-year-old patient and were obtained after surgery. 2. the proteoglycans of chondromas contain only a small amount of keratan sulfate (1.3% of total mucopolysaccharide) and the chondroitin sulfate is composed o ... | 1981 | 6802209 |
| dye-coupled electrode system for the rapid determination of cell populations in polluted water. | we determined cell populations in polluted waters by using a fuel cell-type electrode. the electrode was constructed from a platinum anode, a silver peroxide cathode, and a membrane filter for retaining microorganisms. the principle of cell number determination is based on sensing a redox dye reduced by the microorganisms with the electrode. sample solutions containing microorganisms were membrane filtered, and the resulting filter containing microbial cells was attached to the surface of a plat ... | 1982 | 6805429 |
| endo-beta-galactosidase from flavobacterium keratolyticus. | | 1982 | 6808310 |
| endo-beta-n-acetylglucosaminidase f: endoglycosidase from flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins. | we have detected an endoglycosidase activity produced by flavobacterium meningosepticum. this enzyme, named endo f, cleaves glycans of both the high-mannose and the complex type linked through asparagine to the protein backbone. the data indicate that cleavage occurs via hydrolysis of the glycosidic bond of the n,n'-diacetylchitobiose core structure adjacent to asparagine, similar to that due to endo h and endo d. extreme variability was noted in the availability of this cleavage site among n-li ... | 1982 | 6812050 |
| [properties of aromatic-amino-acid aminotransferases from two chloramphenicol-resistant flavobacteria]. | two enzymes of chloramphenicol-resistant flavobacterium strain cb 60 and strain cb 6 which catalyse the transamination of tyrosine, phenylalanine and tryptophan were enriched 43-fold and 31-fold. the molecular mass for the aromatic-amino-acid aminotransferases of both strains was found to be 120000 da and the isoelectric point was at about ph 4.2-4.3. both enzymes are not influenced by edta. a ping pong bi-bi-mechanism was obtained for the kinetic mechanism of the reaction. the aminotransferases ... | 1982 | 6816714 |
| occurrence of nonfermentative gram-negative rods other than pseudomonas aeruginosa in the respiratory tract of children with cystic fibrosis. | there have been no comprehensive microbiologic studies of the frequency of respiratory colonization with nonfermentative gram-negative rods (nfgnr) other than pseudomonas aeruginosa in patients with cystic fibrosis (cf). records of bacteria isolated from throats and sputa of cf patients of the yale-new haven hospital cf clinic from 1975-1979 were reviewed in order to determine the incidence of these species. thirty-one strains were recovered. twenty patients from the cf clinic (with an average c ... | 1982 | 6820015 |
| the human erythrocyte cryptantigen tk: exposure by an endo-beta-galactosidase from flavobacterium keratolycus. | | 1982 | 6820566 |
| cutaneous flavobacteriosis--polymorphous skin granulomas from flavobacterium capsulatum. a case report. | a case of multiple eruptive skin granulomas caused by flavobacterium capsulatum is described. the organism was resistant or poorly sensitive to all antibiotics except carbenicillin. cure was brought about by using maximal doses of this drug. the source of the infection could not be proved, but it dated from an orthopaedic procedure to the elbow which was followed by a chronic cellulitis at the operation site. since this is an organism known to occur in stored water, it was presumed that the flav ... | 1983 | 6823642 |
| an enzymic creatinine assay and a direct ammonia assay in coated thin films. | we developed a thin-film enzymic assay for creatinine that makes use of creatinine iminohydrolase (ec 3.5.4.21) to convert creatinine to n-methylhydantoin and ammonia. the ammonia diffuses through a semipermeable layer and is quantitated by reaction with bromphenol blue. a paired analysis of the sample on a separate coating without the enzymic reaction measures endogenous ammonia and, for samples with normal concentrations of ammonia, allows accurate determination of serum creatinine to 150 mg/l ... | 1983 | 6831692 |
| microbial fouling of reverse-osmosis membranes used in advanced wastewater treatment technology: chemical, bacteriological, and ultrastructural analyses. | biofouling of reverse-osmosis membranes was investigated at an advanced wastewater treatment facility. cellulose diacetate membranes operated for approximately 4,000 h became uniformly coated with a mucilaginous fouling layer. the fouling material was approximately 93% water by weight, and nearly 90% of the dehydrated residue was organic in composition. calcium, phosphorous, sulfur, and chlorine were the major inorganic constituents detected. protein and carbohydrate represented as much as 30 an ... | 1983 | 6847180 |
| nutritional versatility of a starch-utilizing flavobacterium at low substrate concentrations. | a starch-utilizing yellow-pigmented bacterium, isolated from tap water, was tested for the utilization of 64 natural compounds at a concentration of 1 g/liter by measuring colony growth on agar media. only 12 carbohydrates and glycerol promoted growth. growth experiments with the organism in pasteurized tap water supplied with mixtures of substrates at concentrations of 1 or 10 micrograms of c of each substrate per liter, followed by separate experiments with a number of carbohydrates at 10 micr ... | 1983 | 6847187 |
| model for bacterial culture growth rate throughout the entire biokinetic temperature range. | the "square-root" relationship proposed by ratkowsky et al. (j. bacteriol. 149:1-5, 1982) for modeling the growth rate of bacteria below the optimum growth temperature was extended to cover the full biokinetic temperature range. two of the four parameters of this new nonlinear regression model represent minimum and maximum temperature bounds, respectively, for the predicted growth of the culture. the new model is easy to fit and has other desirable statistical properties. for example, the least- ... | 1983 | 6853443 |
| adhesive responses of fibroblast and neuroblastoma cells to substrata coated with polyvalent or monoclonal antibody to fibronectin. | both polyvalent and hybridoma-produced antibodies to fibronectin (fn) were used to 'map' the immunoaccessible subsets of cell surface fibronectin on virus-transformed murine fibroblast svt2 and rat neuroblastoma b104 cells. as one approach to this end, attachment and spreading responses of cells were measured on tissue culture substrata coated with antibody or with plasma fibronectin to compare their adhesive responses. both svt2 and b104 cells adhere poorly to polyvalent anti-fn-coated substrat ... | 1983 | 6861909 |
| studies on a collection of strains of the genus flavobacterium. 2. nutritional studies. | an attempt has been made to further characterize the flavobacterium groups found in an earlier study by determining utilizable carbon-energy sources. as a preliminary, it was initially tried to define minimal growth factor requirements. it was found likely that most of the flavobacterium groups have amino acid requirements, but a requirement for vitamins was also established for some strains. testing flavobacteria for carbon-energy sources by the replica plating method was found sufficiently tec ... | 1983 | 6869012 |
| marinactan, antitumor polysaccharide produced by marine bacteria. | extracellular polysaccharides of marine bacteria were screened for their antitumor activity against sarcoma-180 solid tumor in mice. an active polysaccharide was purified and named marinactan. the producing microorganism has a typical marine bacterial nature requiring sea water for growth and was identified as flavobacterium uliginosum. marinactan is a novel heteroglycan consisting of glucose, mannose and fucose in a ratio of approximately 7:2:1. marinactan, 10-50 mg mg/kg daily for 10 days i.p. ... | 1983 | 6874564 |
| separation of bacterial ubiquinones by reverse-phase high-pressure liquid chromatography. | a procedure was developed for the separation of ubiquinones by high-pressure liquid chromatography on a reverse-phase c18 column. ubiquinones q6 through q14 were resolved in 20 min and were distinguished from menaquinones by comparing uv spectra at 248 and 275 nm. | 1983 | 6885985 |
| classification of bacteria from commercial egg washers and washed and unwashed eggs. | a total of 432 bacterial isolates from washed and unwashed eggs, egg-washer surfaces, and washwaters from five egg-grading plants in maryland and southeastern pennsylvania were classified. counts on equipment surfaces showed considerable variation from plant to plant, reflecting care used in cleaning. unwashed eggs had a higher percentage of gram-positive cocci (71%), and isolates included streptococcus faecalis, aerococcus, and escherichia coli, which were not isolated from equipment surfaces a ... | 1980 | 6893532 |
| flavobacterium, infective endocarditis and prosthetic heart valve. | | 1980 | 6930276 |
| the laboratory diagnosis of opportunistic infections caused by uncommon bacteria in papua new guinea. | flavobacterium meningosepticum, chromobacterium violaceum and listeria monocytogenes are opportunistic pathogens of man and are occasional but important causes of infections in neonates, infants and adults in papua new guinea. mortality is high. flavobacterium meningosepticum should be suspected when a weak indole producing oxidase positive gram-negative bacillus producing a discrete yellow non-diffusing pigment at room temperature and showing a resistant antibiotic sensitivity pattern is isolat ... | 1981 | 6951339 |
| growth characteristics and pathogenesis of experimental pneumocystis carinii pneumonia. | pneumocystis carinii pneumonia was produced in two groups of rats by the administration of corticosteroids, a low-protein (8%) diet, and tetracycline in the drinking water. a third group not on corticosteroids or a low-protein diet served as controls. members of the first group were sacrificed weekly for 8 weeks, and lungs were examined. a highly significant correlation was found between the histopathological assessment of the intensity of p. carinii infection and the number of cysts counted in ... | 1980 | 6966614 |
| infective endocarditis caused by gram-negative bacteria: a review of the literature, 1945-1977. | | 1980 | 6986059 |
| proline specific endo- and exopeptidases. | peptidases which are specific for proline residues have been described and include endopeptidases (post-proline cleaving enzyme and proline specific endopeptidase), n-terminal exopeptidases (post-proline dipeptidyl aminopeptidase, proline iminopeptidase, aminopeptidase p), c-terminal exopeptidases (prolylcarboxypeptidase, and carboxypeptidase p) and dipeptidases (prolyl dipeptidase and proline dipeptidase). the properties, distinguishing charcteristics, and possible significance of these proline ... | 1980 | 6991912 |
| phosphatidylethanolamine distribution and fluidity in outer and inner membranes of the gram-negative bacterium erwinia carotovora. | 1. the distribution of phosphatidylethanolamine, the major lipid of erwinia carotovora, was investigated in intact bacteria, spheroplasts and outer- and inner-membrane preparations, with the amino-group reagent 2,4,6-trinitrobenzenesulphonic acid. only 4% was found on the external surface of the outer membrane with 30% on the internal surface, whereas the inner membrane had 27 and 38% on its external and internal surfaces respectively. some comparative studies were made with three other bacteri ... | 1980 | 6996674 |
| purification and characterization of polyethylene glycol dehydrogenase involved in the bacterial metabolism of polyethylene glycol. | polyethylene glycol (peg) dehydrogenase in crude extracts of a peg 20,000-utilizing mixed culture was purified 24 times by precipitation with ammonium sulfate, solubilization with laurylbetaine, and chromatography with diethylamino-ethyl-cellulose, hydroxylapatite, and sephadex g-200. the purified enzyme was confirmed to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the molecular weight of the enzyme, which appeared to consist of four identical subunits, was 2.4 x ... | 1980 | 6999995 |
| biotransformation of the pesticide sodium arsenate. | biotransformation is an important parameter in assessing the environmental impact and fate of pesticides since metabolites produced may be either more or less toxic than the parent compound. sodium arsenate (+5 inorganic), the wood preservative and insecticide, may be converted to both inorganic (+3) and organic compounds (-3) by microorganisms in soil, sediment and water bodies. biotransformation of sodium arsenate was studied in pure cultures of 5 bacterial species using a mineral salt and lim ... | 1981 | 7009715 |
| [proline-specific endopeptidase (author's transl)]. | | 1980 | 7010423 |
| in vitro susceptibilities of nonfermentative gram-negative bacilli other than pseudomonas aeruginosa to 32 antimicrobial agents. | the animal inhibitory concentrations of 32 antimicrobial agents for 322 strains of non-fermentative gram-negative bacilli, representing 30 species and unnamed groups other than pseudomonas aeruginosa, were determined in cation-supplemented mueller-hinton broth with use of a microdilution method. the activities of beta-lactam antibiotics varied, but azlocillin, mezlocillin, piperacillin, cefotaxime, and moxalactam consistently were more active than were available penicillins and cephalosporins; t ... | 1980 | 7012987 |
| synthesis of 16 alpha-3h androgen and estrogen substrates for 16 alpha-hydroxylase. | the synthesis of 16 alpha-3h androgens and estrogens is described. 1-(3h)-acetic acid in the presence of zinc dust reacts with 16 alpha-bromo-17-ketosteroids to produce 16 alpha-3h-17-ketosteroids. this chemical reaction was used to prepare 16 alpha-3h-dehydroepiandrosterone (i) and 16 alpha-3h-estrone acetate (xi) from 16 alpha-bromo-dehydroepiandrosterone (x) and from 16 alpha-bromo-estrone acetate (xii), respectively. using appropriate microbiological techniques, it was possible to convert th ... | 1981 | 7013160 |
| enterotoxigenic bacteria in food and water from an ethiopian community. | food and water samples from an ethiopian community were screened for the presence of enterotoxin-producing bacteria. using the chinese hamster ovary cell assay, 40 of 213 isolates (18.8%) produced heat-labile (lt) enterotoxin. these lt-producing isolates comprised 33 of 177 (18.6%) strains from 24 of 68 food samples (35.3%) and 7 of 36 (19.4%) isolates of 4 of 17 water samples (23.5%). one lt-producing strain each of salmonella emek and of shigella dysenteriae was found. three pseudomonads, all ... | 1981 | 7016032 |
| preservation and transportation of bacteria by a simple gelatin disk method. | a wide range of bacterial species, e.g., enterobacteriaceae and neisseria, streptococcus, branhamella, haemophilus, gemella, pseudomonas, flavobacterium, and bacteroides species, were successfully preserved for 1 to 5 years by our gelatin disk drying method. the beta-lactamase activity of penicillinase-producing neisseria gonorrhoeae was retained for more than 3 years with this method. good results were also obtained upon airmailing many strains of n. gonorrhoeae embedded in gelatin disks from j ... | 1981 | 7021588 |
| effects of storage time and temperature on quantitative culture of canine urine. | results of quantitative culture of 25 canine urine samples stored at different temperatures for varying periods (2, 4, 6, 12, and 24 hours) following collection were compared. samples stored at room temperature (21 to 25 c) were unreliable for diagnostic quantitative culture after 24 hours. false-positive results were obtained in 13 (50%) of the samples, and false-negative results were obtained in 1 (4%) sample. likewise, samples sent via the us mail were unreliable for diagnostic quantitative c ... | 1981 | 7024224 |
| [the bacteriostatic and bactericidal effect of amniotic fluid (author's transl)]. | the bacteriostatic and bactericidal effect of 100 samples of amniotic fluid (la) was studied against 5 bacterial species that are responsible for neonatal infections. these results show that there is a bacteriostatic activity in liquor. 52 specimens of liquor amnii (52%) were shown to be active against at least one of the 5 bacterial specimens studied. 32 samples of liquor (32%) were active against listeria monocytogenes, 21 (21%) against flavobacterium meningosepticum, 18 (18%) against escheric ... | 1981 | 7024394 |
| coliform inhibition by bacteriocin-like substances in drinking water distribution systems. | bacterial isolates from an unchlorinated potable groundwater system and a chlorinated surface water system were screened by an agar overlay method for the ability to produce bacteriocin-like substances (bls) inhibitory to the growth of escherichia coli, klebsiella sp., and enterobacter aerogenes. the production of coliform-specific bls by noncoliform bacteria varied with the site and date of isolation as well as the genus of the producer strain. a total of 448 bacterial isolates were screened fr ... | 1981 | 7027953 |
| stereochemistry of beta-, gamma-, and epsilon-ring formation in bacterial c50. | cell-free systems from corynebacterium poinsettiae and micrococcus luteus incorporated labeled mevalonic acids into acyclic c40 and cyclic c50 carotenoids. when (3r,4r)-[2-14c,4-3h1]mevalonate was used as substrate, the 14c:3h ratios of c.p.450 and sarcinaxanthin showed that the hydrogen atoms at c-2 of both carotenoids, and that at c-6 of sarcinaxanthin, are derived from the 4-pro-r position of mevalonate. the 14c:3h ratios of c.p.450 and sarcinaxanthin synthesized from (2rs,3r)-[2-14c,2-3h2]me ... | 1981 | 7028738 |
| sensitivity, specificity, and reproducibility of the automicrobic system (with the enterobacteriaceae-plus biochemical card) for identifying clinical isolates of gram- negative bacilli. | two independent laboratories tested 1,743 clinical isolates by using the enterobacteriaceae-plus biochemical card in the automicrobic system (ams) and identical standard reference methods. included were 55 isolates representing 11 species that cannot be identified by the enterobacteriaceae-plus biochemical card computer program; 3 or these isolates were incorrectly identified as pseudomonas cepacia. with the other 1,688 isolates, 5% of the ams identifications were considered to be equivocal (pro ... | 1982 | 7040457 |
| [enzymes and genetic informations of hydrolases active on nylon oligomer, unnatural substrates (author's transl)]. | | 1982 | 7043655 |