| [isolation, identification and degradation characteristics of bisphenol a degrading strain]. | a bisphenol a(bpa) degrading strain b-16 was isolated from compost leachate of municipal solid waste. according to the morphological characteristics, physiological and biochemical, and sequence analysis of 16s rdna, the strain was identified preliminarily as achromobacter xylosoxidans. by tests in shaking flasks, the effects of the conditions of growth was studied, and it was determined that the optimum conditions were inoculum amount 0.6%, ph 7.0, and 30 degrees c. under these conditions, when ... | 2006 | 17256617 |
| insights into the mechanism of n2o reduction by reductively activated n2o reductase from kinetics and spectroscopic studies of ph effects. | nitrous oxide reductase (n2or) from achromobacter cycloclastes (ac) can be reductively activated with reduced methyl viologen over a broad range of ph. activated ac n2or displays a complex activity profile as a function of the ph at which catalytic turnover is measured. spectroscopic and steady-state kinetics data suggest that [h+] has multiple effects on both the activation and the catalytic reactions. these experimental results are in good agreement with previous theoretical studies, which sug ... | 2007 | 17257001 |
| veb-1 in achromobacter xylosoxidans from cystic fibrosis patient, france. | | 2006 | 17283625 |
| aerobic degradation of bisphenol a by achromobacter xylosoxidans strain b-16 isolated from compost leachate of municipal solid waste. | a novel bacterium designated strain b-16 was isolated from the compost leachate of the municipal solid waste (msw) in a laboratory reactor. this strain was identified as a gram-negative bacterium, achromobacter xylosoxidans that could grow on bisphenol a (bpa, a representative endocrine disruptor) as a sole carbon source under aerobic condition. bpa-degrading characteristics of strain b-16 were investigated in liquid cultures. the results show that bpa degradation was influenced by several facto ... | 2007 | 17291567 |
| microbiological changes in naturally fermented cassava fish (pseudotolithus sp.) for lanhouin production. | cassava fish (pseudotolithus sp.) was naturally fermented on three different occasions at room temperature (28-30 degrees c) for 3 to 8 days and the microbial population and the occurrence of various bacterial species were monitored. in general, after a slight increase during the early stages of fermentation, the microbial population decreased as the fermentation progressed. a total of 224 isolates belonging to the genera bacillus, staphylococcus, micrococcus, streptococcus, corynebacterim, pseu ... | 2007 | 17291615 |
| emerging cystic fibrosis pathogens: incidence and antimicrobial resistance. | we examined the frequency of isolation and the antimicrobial resistance of burkholderia cepacia complex, stenotrophomonas maltophilia and achromobacter xylosoxidans in cystic fibrosis patients from 2000 to 2004. strains susceptibility to tobramycin, piperacillin/tazobactam, imipenem, gentamicin, ciprofloxacin and ceftazidime was determined by disc diffusion assay. b. cepacia complex showed a very high resistance also to ciprofloxacin reaching 100% in 2004. s. maltophilia and a. xvylosoxidans sho ... | 2007 | 17319602 |
| spectroscopic, computational, and kinetic studies of the mu4-sulfide-bridged tetranuclear cuz cluster in n2o reductase: ph effect on the edge ligand and its contribution to reactivity. | a combination of spectroscopy and density functional theory (dft) calculations has been used to evaluate the ph effect at the cuz site in pseudomonas nautica (pn) nitrous oxide reductase (n2or) and achromobacter cycloclastes (ac) n2or and its relevance to catalysis. absorption, magnetic circular dichroism, and electron paramagnetic resonance with sulfur k-edge x-ray absorption spectra of the enzymes at high and low ph show minor changes. however, resonance raman (rr) spectroscopy of pnn2or at hi ... | 2007 | 17352474 |
| culturable bacteria present in the fluid of the hooded-pitcher plant sarracenia minor based on 16s rdna gene sequence data. | the culturable microbial community within the pitcher fluid of 93 sarracenia minor carnivorous plants was examined over a 2-year study. many aspects of the plant/bacterial/insect interaction within the pitcher fluid are minimally understood because the bacterial taxa present in these pitchers have not been identified. thirteen isolates were characterized by 16s rdna sequencing and subsequent phylogenetic analysis. the proteobacteria were the most abundant taxa and included representatives from s ... | 2007 | 17380356 |
| presumed pseudobacteremia outbreak resulting from contamination of proportional disinfectant dispenser. | reported here are the microbiological and epidemiological details of a presumed outbreak of aerobic gram-negative bacilli infections affecting 19 hematological patients, which was traced to contaminated disinfectant. over a 5-month period, the following organisms were isolated from the blood cultures of 19 neutropenic patients: pseudomonas fluorescens (n = 13), achromobacter xylosoxidans (n = 12), comamonas testosteroni (n = 2) or stenotrophomonas maltophilia (n = 1). the affected patients were ... | 2007 | 17393202 |
| [isolation of achromobacter xylosoxidans ns12 and degradation of nitrophenols]. | a nitrophenols-degrading bacterium, strain ns12, was isolated from a mangrove sediment by enrichment culture under aerobic conditions. based on the analysis of 16s rdna gene sequence the isolate was identified as achromobacter xylosoxidans strain ns12 was able to metabolize both o-nitrophenol (onp) and p-nitrophenol (pnp) as the sole source of carbon, energy and nitrogen. however, this strain was not able to use 3-nitrophenol (mnp) as the only source of carbon energy and nitrogen for growth. the ... | 2007 | 17489210 |
| evaluation of the colorimetric vitek 2 card for identification of gram-negative nonfermentative rods: comparison to 16s rrna gene sequencing. | ninety strains of a collection of well-identified clinical isolates of gram-negative nonfermentative rods collected over a period of 5 years were evaluated using the new colorimetric vitek 2 card. the vitek 2 colorimetric system identified 53 (59%) of the isolates to the species level and 9 (10%) to the genus level; 28 (31%) isolates were misidentified. an algorithm combining the colorimetric vitek 2 card and 16s rrna gene sequencing for adequate identification of gram-negative nonfermentative r ... | 2007 | 17507509 |
| outbreak of achromobacter xylosoxidans pseudobacteremia in a neonatal care unit related to contaminated chlorhexidine solution. | | 2007 | 17516103 |
| emerging and unusual gram-negative infections in cystic fibrosis. | people with cystic fibrosis (cf) have chronic airway infection and frequent exposure to antibiotics, which often leads to the emergence of resistant organisms. in addition to the development of multiresistance in common cf pathogens such as pseudomonas aeruginosa, several newer, inherently resistant gram-negative species are becoming more common, including burkholderia cepacia complex, stenotrophomonas maltophilia, achromobacter (alcaligenes) xylosoxidans, certain ralstonia species, and those wi ... | 2007 | 17562501 |
| new enzymatic method of chiral amino acid synthesis by dynamic kinetic resolution of amino acid amides: use of stereoselective amino acid amidases in the presence of alpha-amino-epsilon-caprolactam racemase. | d- and l-amino acids were produced from l- and d-amino acid amides by d-aminopeptidase from ochrobactrum anthropi c1-38 and l-amino acid amidase from pseudomonas azotoformans iam 1603, respectively, in the presence of alpha-amino-epsilon-caprolactam racemase from achromobacter obae as the catalyst by dynamic kinetic resolution of amino acid amides. | 2007 | 17586677 |
| patient risk of contact with respiratory pathogens from inanimate surfaces in a cystic fibrosis outpatient clinic. a prospective study over a four-year period. | acquisition of respiratory pathogens such as pseudomonas aeruginosa (pa) is associated with increased morbidity and mortality in cystic fibrosis (cf). research on the prevalence of these pathogens on environmental surfaces of a cf center is scanty, and so far no study has determined what risk cf patients have of coming in contact with them during their visits to the cf center. this study is aimed at assessing the prevalence of some respiratory pathogens in samples taken systematically during a 4 ... | 2007 | 17639586 |
| endophytic bacteria in sunflower (helianthus annuus l.): isolation, characterization, and production of jasmonates and abscisic acid in culture medium. | this study was designed to isolate and characterize endophytic bacteria from sunflower (helianthus annuus) grown under irrigation and water stress (drought) conditions, to analyze growth of isolated bacteria under drought condition, and to evaluate the ability of bacteria isolated from plants cultivated under drought to produce jasmonates (jas) and abscisic acid (aba). bacteria were isolated from soil samples collected when sunflower plants were at the end of the vegetative stage. a total of 29 ... | 2007 | 17657487 |
| anaerobic purification, characterization and preliminary mechanistic study of recombinant nitrous oxide reductase from achromobacter cycloclastes. | an overexpression system for nitrous oxide reductase (n(2)or), an enzyme that catalyzes the conversion of n(2)o to n(2) and h(2)o, has been developed in achromobacter cycloclastes. anaerobically purified a. cycloclastes recombinant n(2)or (acn(2)or) has on average 4.5 cu and 1.2 s per monomer. upon reduction by methyl viologen, acn(2)or displays a high specific activity: 124 u/mg at 25 degrees c. anaerobically purified acn(2)or displays a unique absorption spectrum. uv-visible and epr spectra, c ... | 2007 | 17681606 |
| survival of lung transplant patients with cystic fibrosis harboring panresistant bacteria other than burkholderia cepacia, compared with patients harboring sensitive bacteria. | the impact of panresistant bacteria, other than burkholderia cepacia, on the survival after lung transplantation in patients with cystic fibrosis (cf) remains controversial. | 2007 | 17692788 |
| genomic analysis reveals widespread occurrence of new classes of copper nitrite reductases. | recently, the structure of a cu-containing nitrite reductase (nir) from hyphomicrobium denitrificans (hdnir) has been reported, establishing the existence of a new family of cu-nir where an additional type 1 cu (t1cu) containing cupredoxin domain is located at the n-terminus (nojiri et al. in proc. natl. acad. sci. usa 104:4315-4320, 2007). hdnir retains the well-characterised coupled t1cu-type 2 cu (t2cu) core, where the t2cu catalytic site is also built utilising ligands from neighbouring mono ... | 2007 | 17712582 |
| urinary tract infection due to achromobacter xylosoxidans: report of 9 cases. | urinary tract infection (uti) due to achromobacter xylosoxidans is rare. the aims were to know the frequency and clinical characteristics of this infection in our area. we performed a retrospective analysis of 9 patients with uti caused by this organism diagnosed over a period of 13 y. the mean age was 63.1 y. all patients had underlying diseases or urological abnormalities. the most frequent underlying diseases were solid or hematological malignancies (3 cases). seven patients (77.7%) had urolo ... | 2008 | 17852927 |
| [ochrobactrum anthropi bacteremia developed after cholangiopancreatography]. | ochrobactrum anthropi (formerly achromobacter spp.) is an aerobic, motile, oxidase positive and lactose negative gram negative bacillus which is widely distributed in the environment and water sources. in recent publications, o. anthropi has an increasing importance as a nosocomial infection agent. the aim of this report was to present a case of o. anthropi bacteremia developed after endoscopic retrograde cholangiopancreatography (ercp). a 89-year old female patient presented with high fever one ... | 2007 | 17933261 |
| characterization of novel linuron-mineralizing bacterial consortia enriched from long-term linuron-treated agricultural soils. | linuron-mineralizing cultures were enriched from two linuron-treated agricultural soils in the presence and absence of a solid support. the cultures contained linuron-degrading bacteria, which coexisted with bacteria degrading either 3,4-dichloroaniline (3,4-dca) or n,o-dimethylhydroxylamine (n,o-dmha), two common metabolites in the linuron degradation pathway. for one soil, the presence of a solid support enriched for linuron-degrading strains phylogenetically related to but different from thos ... | 2007 | 17991021 |
| iatrogenic hemolytic anemia and endocarditis in new zealand white rabbits secondary to achromobacter xylosoxidans infection. | during a 3-mo period, 9 of the 15 new zealand white rabbits used in a heart failure study developed a hemolytic anemia. the heart failure model involved the creation of an aortic insufficiency (ai) followed 2 to 6 wk later by the creation of an aortic stenosis (as). none of the 9 animals that developed hemolytic anemia responded to medical management, and 6 of the 9 were euthanized for humane concerns. necropsies and blood cultures were performed on all anemic animals; 7 of these cultures yielde ... | 2007 | 17994675 |
| purification and characterization of arylmalonate decarboxylase from achromobacter sp. ku1311. | we have isolated, purified and characterized arylmalonate decarboxylase (amdase; ec 4.1.1.76). this is an unique enzyme that gives optically pure arylpropionates from the corresponding arylmalonates. recently, we have screened similar enzyme producers from soil samples and succeeded in isolating achromobacter sp. ku1311. the gene encoding the enzyme was cloned and sequenced. the amdase gene consists of 720 nucleotides, which specifies a 240 amino acid protein with a relative molecular mass of 24 ... | 2007 | 18023797 |
| nucleotide sequence, organization and characterization of the (halo)aromatic acid catabolic plasmid pa81 from achromobacter xylosoxidans a8. | the complete 98,192bp nucleotide sequence was determined for plasmid pa81, which is harbored by the haloaromatic acid-degrading bacterium achromobacter xylosoxidans a8. the majority of the 103 open reading frames identified on pa81 could be categorized as either "backbone" genes, genes encoding (halo)aromatic compound degradation, or heavy metal resistance determinants. the backbone genes controlled conjugative transfer, replication and plasmid stability, and were well conserved with other incp1 ... | 2008 | 18249097 |
| [continuous intraspinal ceftazidime administration in a case for treatment of purulent meningitis]. | to investigate the feasibility of continuous intraspinal ceftazidime administration for treatment of purulent meningitis due to achromobacter infection. | 2008 | 18250049 |
| screening, cloning, expression, and purification of an acidic arylmalonate decarboxylase from enterobacter cloacae ku1313. | we have already isolated, purified, and characterized arylmalonate decarboxylases (amdase; ec. 4.1.1.76) from alcaligenes bronchisepticus ku1201 and achromobacter sp. ku1311. these are unique enzymes that give optically pure alpha-arylpropionates from the corresponding alpha-aryl-alpha-methylmalonates. recently, we have further screened novel amdase producers from soil samples under acidic conditions and succeeded in isolating enterobacter cloacae ku1313. the gene encoding the enzyme was cloned ... | 2008 | 18283449 |
| inoculation of plant growth promoting bacterium achromobacter xylosoxidans strain ax10 for the improvement of copper phytoextraction by brassica juncea. | in this study, a copper-resistant plant growth promoting bacterial (pgpb) strain ax10 was isolated from a cu mine soil to assess its plant growth promotion and copper uptake in brassica juncea. the strain ax10 tolerated concentrations up to 600 mg cul(-1) on a luria-bertani (lb) agar medium and utilized 1-aminocyclopropane-1-carboxylic acid (acc) as a sole n source in df salts minimal medium. the strain ax10 was characterized as achromobacter xylosoxidans based on its 16s rdna sequence homology ... | 2009 | 18329785 |
| achromobacter xylosoxidans subsp. xylosoxidans prosthetic aortic valve infective endocarditis and aortic root abscesses. | we report a case of prosthetic valve infective endocarditis and aortic root abscesses caused by achromobacter xylosoxidans subsp. xylosoxidans. the patient was an intravenous drug user and had injected amphetamines using 'duck pond water' as a diluent. after surgical intervention and 6 weeks of intravenous meropenem therapy, the patient made an uneventful recovery. | 2008 | 18349376 |
| characterization of a naturally occurring class d beta-lactamase from achromobacter xylosoxidans. | a chromosomally encoded class d beta-lactamase, oxa-114, was characterized from achromobacter xylosoxidans strain cip69598. beta-lactamase oxa-114 shared 56% amino acid identity with the naturally occurring class d beta-lactamase of burkholderia cenocepacia and 42% identity with the acquired oxacillinases oxa-9 and oxa-18. oxa-114 has a narrow-spectrum hydrolysis profile, although it includes imipenem, at a very low level. pcr and sequencing revealed that bla(oxa-114)-like genes were identified ... | 2008 | 18362192 |
| extracellular yeast-lytic enzyme of the bacterium lysobacter sp. xl 1. | an enzyme exhibiting yeast-lytic activity has been isolated from the culture liquid of the bacterium lysobacter sp. xl 1. the optimal conditions for the hydrolysis of saccharomyces cerevisiae cells by the enzyme have been established: 0.15 m sodium acetate buffer, ph 6.0, 50 degrees c. the yeast-lytic activity of the enzyme is inhibited by edta, p-chloromercuribenzoate, and phenylmethylsulfonyl fluoride. according to the data of sds-page, the molecular weight of the protein is 36 kd. the enzyme ... | 2008 | 18393767 |
| bacterial ectosymbionts and virulence silencing in a fusarium oxysporum strain. | in the present article we have ascertained the presence of a consortium of ectosymbiotic bacteria belonging to serratia, achromobacter, bacillus and stenotrophomonas genera associated to the mycelium of the antagonistic fusarium oxysporum msa 35 [wild-type (wt) strain]. morphological characterization carried out on the wt strain, on the f. oxysporum msa 35 without ectosymbionts [cured (cu) strain] and on the pathogenic f. oxysporum f.sp. lactucae (fuslat 10) showed that the ectosymbionts, presen ... | 2008 | 18397306 |
| [(3)h]adenine is a suitable radioligand for the labeling of g protein-coupled adenine receptors but shows high affinity to bacterial contaminations in buffer solutions. | [(3)h]adenine has previously been used to label the newly discovered g protein-coupled murine adenine receptors. recent reports have questioned the suitability of [(3)h]adenine for adenine receptor binding studies because of curious results, e.g. high specific binding even in the absence of mammalian protein. in this study, we showed that specific [(3)h]adenine binding to various mammalian membrane preparations increased linearly with protein concentration. furthermore, we found that tris-buffer ... | 2007 | 18404448 |
| a pyrene-degrading consortium from deep-sea sediment of the west pacific and its key member cycloclasticus sp. p1. | a pyrene-degrading bacterial consortium was obtained from deep-sea sediments of the pacific ocean. the consortium degraded many kinds of polycyclic aromatic hydrocarbons (pahs), including naphthalene, phenanthrene, pyrene, acenaphthene, fluorene, anthracene, fluoranthene, 2-methylnaphthalene and 2,6-dimethylnaphthalene, but it did not grow with chrysene and benzo[alpha]pyrene. with methods of plate cultivation and polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge), 72 b ... | 2008 | 18430013 |
| fourier transform infrared spectroscopy for rapid identification of nonfermenting gram-negative bacteria isolated from sputum samples from cystic fibrosis patients. | the accurate and rapid identification of bacteria isolated from the respiratory tract of patients with cystic fibrosis (cf) is critical in epidemiological studies, during intrahospital outbreaks, for patient treatment, and for determination of therapeutic options. while the most common organisms isolated from sputum samples are pseudomonas aeruginosa, staphylococcus aureus, and haemophilus influenzae, in recent decades an increasing fraction of cf patients has been colonized by other nonfermenti ... | 2008 | 18550747 |
| isolation and description of a stable carbazole-degrading microbial consortium consisting of chryseobacterium sp. ncy and achromobacter sp. ncw. | a stable microbial consortium, separated from a refinery wastewater sample, was able to utilize carbazole as the sole source of carbon, nitrogen, and energy, and liberated ammonia from excess nitrogen. two bacterial strains (ncy and ncw) were isolated from the microbial consortium using a nutrient agar plate. based on the 16s rdna sequence analysis, the two bacteria were identified as chryseobacterium sp. ncy and achromobacter sp. ncw, respectively. no intermediates of carbazole degradation were ... | 2008 | 18584242 |
| community-based degradation of 4-chorosalicylate tracked on the single cell level. | 4-chlorosalicylate (4-cs) can be degraded completely by a bacterial consortium consisting of pseudomonas reinekei (mt1), achromobacter spanius (mt3) and pseudomonas veronii (mt4). the fourth species wautersiella falsenii (mt2) is thought to act as a 'necrotizer' of the community. single cell approaches were used to follow every species' degradation activity within the community by assuming that growth and proliferation are activity markers for the utilization of 4-cs and its degradation pathway ... | 2008 | 18593643 |
| a new procedure for enzymatic semisynthesis of human insulin by hydrolysis of single-chain des-(b-30)-lnsulin precursor with lysyl endopeptidase. | it has been shown that the single-chain des-(b-30)-insulin precursor (sci) can be converted into human insulin ester by transpeptidation using trypsin in the presence of a threonine derivative. the present study demonstrates that achromobacter lyticus protease 1 (lysyl endopeptidase) can catalyze the transpeptidation reaction more efficiently than can trypsin. it is also shown that des-(b-30)-insulin (dai) can be produced by hydrolysis of sci with the lysyl endopeptidase. since it is well known ... | 1991 | 18600661 |
| identification and management of unusual pathogens in cystic fibrosis. | | 2008 | 18607011 |
| isolation and characterization of acc deaminase gene from two plant growth-promoting rhizobacteria. | lowering of plant ethylene by deamination of its immediate precursor 1-aminocyclopropane-1-carboxylate (acc) is a key trait found in many rhizobacteria. we isolated and screened bacteria from the rhizosphere of wheat for their acc-degrading ability. the acc deaminase gene (acds) isolated from two bacterial isolates through pcr amplification was cloned and sequenced. nucleotide sequence alignment of these genes with previously reported genes of pseudomonas sp. strain acp and enterobacter cloacae ... | 2008 | 18654819 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients. | the identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (cf) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. these techniques remain time-consuming, expensive, and technically demanding. we used a method based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof-ms) for the identification of these bacteria. a set of reference strains belonging to 58 species of clinically relevan ... | 2008 | 18685005 |
| [detection methods and changes in metallo-beta-lactamase-producing gram-negative rod isolates at nagasaki university hospital from 1991 to 2005]. | since plasmid-mediated metallo-beta-lactamase (mbl)-producing pseudimonas aeruginosa was reported in japan, mbl-producing gram-negative rods (gnrs) have emerged worldwide. we developed a way to detect mbl-producing gnrs in routine examination using broth microdilution with the mbl inhibitor sodium mercaptoacetate (sma) in frozen plates. between 1996 and 2005, we evaluated this and other methods, including broth microdilution with another mbl inhibitor dipicolinic acid (dpa) in dry plates, conven ... | 2008 | 18697478 |
| [isolation and characterization of a new glyphosate-resistant strain from extremely polluted environment]. | to isolate and characterize a glyphosate-resistant strain from extremely polluted environment. | 2008 | 18720850 |
| amino acid sequence of a kinin-releasing enzyme, kr-e-1, from the venom of agkistrodon caliginosus (kankoku-mamushi). | the amino acid sequence of a bradykinin-releasing enzyme, named kr-e-1, isolated from the venom of agkistrodon caliginosus (kankoku-mamushi) was determined by edman sequencing of the peptides which was derived from digests with cyanogen bromide, hydroxylamine, achromobacter protease i, trypsin, v8 protease, arginine endopeptidase, and endoproteinase asp-n. kr-e-1 consisted of 235 amino acids and showed conservation of the catalytic amino acid residues (his(57), asp(102), and ser(195)) of the chy ... | 2008 | 18723043 |
| emerging pathogens in cystic fibrosis: ten years of follow-up in a cohort of patients. | in patients with cystic fibrosis (cf), there is an increasing incidence of some uncommon respiratory pathogens, such as burkholderia cepacia complex (bcc), stenotrophomonas maltophilia, and achromobacter xylosoxidans. in order to evaluate the prevalence and the clinical impact of these pathogens, we retrospectively studied a total of 109 patients followed in our center from 1996 to 2006 and reviewed the results of 1,550 sputum samples. the isolation of pseudomonas aeruginosa slightly decreased o ... | 2009 | 18758832 |
| a comparative study of avian bordetella-like strains, bordetella bronchiseptica, alcaligenes faecalis and other related nonfermentable bacteria. | cultural and biochemical characteristics, electrophoretic patterns of soluble proteins, and pathogenicity in turkey poults, of 30 avian gram-negative nonfermentable bacterial strains acquired from various geographical regions, of which 26 had been isolated from turkeys and one each from a chicken, duck, goose and sharp-tailed munia, were compared to those of bordetella bronchiseptica, alcaligenes faecalis, alcaligenes denitrificans, achromobacter xylosoxidans and bordetella parapertussis. with t ... | 1983 | 18766782 |
| thermotemporal dynamics of contaminant bacteria and antimicrobials in extended porcine semen. | bacterial contamination of extended porcine semen has been associated with deleterious effects on both semen quality and sow fertility. retrospective, prospective and in vitro studies were performed to delineate the prevalence and behavior of certain bacterial contaminants in extended semen, and antimicrobial pharmacodynamics in various semen diluents. retrospective review of extended semen samples submitted from north american boar studs for microbiological screening at the university of pennsy ... | 2008 | 18774598 |
| diversity of bacteria that nodulate prosopis juliflora in the eastern area of morocco. | a total of 274 bacterial strains were isolated from the root nodules of prosopis juliflora, growing in two arid soils of the eastern area of morocco. a physiological plate screening allowed the selection of 15 strains that could tolerate nacl concentrations between 175 and 500 mm. these were compared with 15 strains chosen from among the ones which did not tolerate high salinity. the diversity of strains was first assessed by rep-pcr amplification fingerprinting using boxa1r and eric primers. an ... | 2008 | 18789623 |
| isolation of microorganisms and substances inhibitory to aflatoxin production. | aflatoxins are toxic and carcinogenic secondary metabolites produced by aspergillus flavus and a. parasiticus. the contamination of crops, feeds, and foods with aflatoxins can have serious effects on the health of humans and animals. although many studies have been done to develop aflatoxin-control strategies, most are limited in their effectiveness. as part of an effort to develop control procedures, we have devised simple and safe methods that are useful for identifying microorganisms that eff ... | 2008 | 18798037 |
| the missing link: bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic bordetellae. | bordetella petrii is the only environmental species hitherto found among the otherwise host-restricted and pathogenic members of the genus bordetella. phylogenetically, it connects the pathogenic bordetellae and environmental bacteria of the genera achromobacter and alcaligenes, which are opportunistic pathogens. b. petrii strains have been isolated from very different environmental niches, including river sediment, polluted soil, marine sponges and a grass root. recently, clinical isolates asso ... | 2008 | 18826580 |
| the preparation of a lipidic endotoxin affects its biological activities. | bacterial membrane constituents, such as ornithine-containing lipid (ol) and the lipid a portion of lipopolysaccharide, trigger various immune responses through recognition by toll-like receptor (tlr) 4. usually, these lipids are dissolved in a small amount of aqueous or organic solvent before being added to the culture medium for examination of their biological activities. macrophages stimulated with ol or lipid a sonically dissolved in saline released both interleukin-1beta (il-1beta) and tumo ... | 2008 | 18827361 |
| [the spectrum of microbiological agents causing pulmonary malt-type lymphomas. a 16s rrna-based analysis of microbial diversity]. | for several anatomical localisations of extranodal marginal zone b-cell lymphoma of malt type (emzbcl), an association with chronic inflammation caused by microbiological agents (e.g. helicobacter pylori in the stomach) has been described. in the lung, a link between lymphomagenesis and a defined causative organism is still missing. a comprehensive diversity survey using 16s-rdna library construction followed by restriction fragment length polymorphism (rflp) analysis, sequencing, and phylogenet ... | 2008 | 18854998 |
| risa-hplc analysis of lung bacterial colonizers of cystic fibrosis children. | microbiological analysis of sputum samples, from children affected by cystic fibrosis (cf) and showing signs of acute or chronic infections, is routinely performed by culture-dependent approaches involving selective media and biochemical tests. these identification schemes are time-consuming, and may lead to false negative results. the aim of this work was to evaluate the efficacy of a ribosomal intergenic spacer analysis (risa) coupled to high performance liquid chromatography (hplc) for the de ... | 2009 | 18929602 |
| pi-interaction tuning of the active site properties of metalloproteins. | the influence of pi-interactions with a his ligand have been investigated in a family of copper-containing redox metalloproteins. the met16phe and met16trp pseudoazurin, and leu12phe spinach and leu14phe phormidium laminosum plastocyanin variants possess active-site pi-contacts between the introduced residue and his81 and his87/92 respectively. the striking overlap of the side chain of phe16 in the met16phe variant and that of met16 in wild type pseudoazurin identifies that this position provide ... | 2008 | 18939838 |
| in vitro activities of a novel nanoemulsion against burkholderia and other multidrug-resistant cystic fibrosis-associated bacterial species. | respiratory tract infection, most often involving opportunistic bacterial species with broad-spectrum antibiotic resistance, is the primary cause of death in persons with cystic fibrosis (cf). species within the burkholderia cepacia complex are especially problematic in this patient population. we investigated a novel surfactant-stabilized oil-in-water nanoemulsion (nb-401) for activity against 150 bacterial isolates recovered primarily from cf respiratory tract specimens. these specimens includ ... | 2009 | 18955531 |
| [microbial degradation of organophosphonates by soil bacteria]. | bacteria that can utilize glyphosate (gp) or methylphosphonic acid (mpa) as a sole phosphorus source have been isolated from soil samples polluted with organophosphonates (op). no matter which of these compounds was predominant in the native habitat of the strains, all of them utilized methylphosphonate. some of the strains isolated from gp-polluted soil could utilize both phosphorus sources. strains growing on glyphosate only were not isolated. the isolates retained high destructive activity af ... | 2008 | 19004352 |
| molecular characterization of lysine 6-dehydrogenase from achromobacter denitrificans. | an inducible lysine 6-dehydrogenase (lys 6-dh), which catalyzes the oxidative deamination of the 6-amino group of l-lysine in the presence of nad(+), was purified to homogeneity from achromobacter denitrificans, yielding a homodimeric protein of 80 kda. the enzyme was specific for the substrate l-lysine and nad(+) served as a cofactor. the dimeric enzyme associated into a hexamer in the presence of 10 mm l-lysine. the k(m) values for l-lysine and nad(+) were 5.0 and 0.09 mm, respectively. the ly ... | 2008 | 19017491 |
| achromobacter xylosoxidans respiratory tract infections in cystic fibrosis patients. | achromobacter xylosoxidans is a ubiquitous gram-negative non-fermenting rod, recently characterized as an emerging pathogen in cystic fibrosis (cf) patients. its pathogenic potential and prevalent transmission routes are still unclear. this study investigated the pfge genetic pattern and antimicrobial resistance profile of 42 a. xylosoxidans isolates obtained over 4 years from the respiratory tract of 22 cf patients. by genotypic analysis, 31 isolates were attributed to 8 distinct pfge patterns ... | 2008 | 19024606 |
| total synthesis of desb30 insulin analogues by biomimetic folding of single-chain precursors. | insulin is a peptide hormone consisting of 51 amino acids in two chains with three disulfide bridges. human insulin and various analogues are used for the treatment of diabetes and are produced recombinantly at ton scale. herein, we report the chemical synthesis of insulin by the step-wise, fmoc-based, solid-phase synthesis of single-chain precursors with solubilising extensions, which under redox conditions, spontaneously fold with the correct pairing of the three disulfide bridges. the folded, ... | 2008 | 19035371 |
| alcaligenes xylosoxidans endophthalmitis following phacoemulsification and intraocular lens implantation. | five consecutive cases of endophthalmitis that developed after cataract extraction by a single surgeon using the same operating room during one morning session are described. following preoperative topical administration of ciprofloxacin, surgery consisted of phacoemulsification with peristaltic pump and fluid venting, polymethylmethacrylate intraocular lens implantation, and corneal suture. no complications occurred during surgery. all five patients developed endophthalmitis caused by infection ... | 2008 | 19065983 |
| biodegradation of methyl tert-butyl ether using bacterial strains. | prospective methyl tert-butyl ether (mtbe) degrading bacterial strains and/or consortia were identified. the potential for aerobic degradation of mtbe was examined using bacterial isolates from contaminated soils and groundwater. using the 16s rdna protocol, two isolates capable of degrading mtbe (rhodococcus pyridinivorans 4a and achromobacter xylosoxidans 6a) were identified. the most efficient consortium of microorganisms was acquired from contaminated groundwater. the growth of both strains ... | 2008 | 19085075 |
| achromobacter keratopathy. | | 2009 | 19118705 |
| effect of flagella expression on adhesion of achromobacter piechaudii to chalk surfaces. | to examine flagella role and cell motility in adhesion of achromobacter piechaudii to chalk. | 2008 | 19120648 |
| genes involved in arsenic transformation and resistance associated with different levels of arsenic-contaminated soils. | arsenic is known as a toxic metalloid, which primarily exists in inorganic form [as(iii) and as(v)] and can be transformed by microbial redox processes in the natural environment. as(iii) is much more toxic and mobile than as(v), hence microbial arsenic redox transformation has a major impact on arsenic toxicity and mobility which can greatly influence the human health. our main purpose was to investigate the distribution and diversity of microbial arsenite-resistant species in three different a ... | 2009 | 19128515 |
| degradation behaviors of nonylphenol ethoxylates by isolated bacteria using improved isolation method. | nonylphenol ethoxylate (npeo)-degrading bacteria were isolated from activated sludge using an improved isolation method, and the corresponding degradation behaviours were investigated. eight npeo-degrading strains distributed in genera pseudomonas, sphingomonas, sphingobium, cupriavidus, ralstonia, achromobacter, and staphylococcus were acquired. the latter five genera have never been reported for the degradation of npeos. four degradation patterns were observed for the eight pure strains. in pa ... | 2008 | 19143306 |
| degradation and mineralization of 2-chloro-, 3-chloro- and 4-chlorobiphenyl by a newly characterized natural bacterial strain isolated from an electrical transformer fluid-contaminated soil. | a bacterium classified as achromobacter xylosoxidans strain ir08 by phenotypic typing coupled with 16s rrna gene analysis was isolated from a soil contaminated with electrical transformer fluid for over sixty years using aroclor 1221 as an enrichment substrate. the substrate utilization profiles revealed that ir08 could grow on all three monochlorobiphenyls (cbs), 2,4'- and 4,4'-dichlorobiphenyl as well as 2-chlorobenzoate (2-cba), 3-cba, 4-cba, and 2,3-dichlorobenzoate. unusually, growth was po ... | 2008 | 19143351 |
| [comparison of microbial community structure in mbrs treating different wastewater]. | in order to investigate the microbial community structures in different membrane bioreactors, total bacterial genomic dna was extracted from biomass in four mbrs treating different wastewater. the microbial community structures were studied by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) and cloning-sequencing. furthermore, and the sequences were used for homology analysis and then two phylogenetic trees were constructed. results indicate that each mbr owns its sp ... | 2008 | 19143399 |
| the novel structure of a pyridoxal 5'-phosphate-dependent fold-type i racemase, alpha-amino-epsilon-caprolactam racemase from achromobacter obae. | alpha-amino-epsilon-caprolactam (acl) racemase (aclr) from achromobacter obae catalyzes the interconversion of l- and d-acl. aclr belongs to the fold-type i group of pyridoxal 5'-phosphate (plp) dependent enzymes. in this study, the first crystal structures of a fold-type i racemase are solved for the native form and epsilon-caprolactam-complexed form of aclr at 2.21 and 2.40 a resolution, respectively. based on the location of epsilon-caprolactam in the complex structure, the substrate-binding ... | 2009 | 19146406 |
| new insights into the biodegradation of thiodiglycol, the hydrolysis product of yperite (sulfur mustard gas). | to isolate thiodiglycol (tdg)-degrading bacteria, the mustard gas hydrolysis product, and to characterize the metabolites formed and the enzymes involved in the degradation. | 2009 | 19191966 |
| anaerobic reduction of hexavalent chromium by bacterial cells of achromobacter sp. strain ch1. | hexavalent chromium [cr(vi)] is a widespread environmental contaminant. achromobacter sp. strain chi was a cr(vi) reducing bacterium with high reduction performance. cr(vi) reductase was just existing in the cells, but was not discharged into the surrounding medium. cr(vi) reduction was carried out with resting cells of strain ch1 under anaerobic conditions. initial ph value and lactate (electron donor) concentration were found to influence the reduction rate of cr(vi), and the optimal condition ... | 2008 | 19216102 |
| isolation and characterization of biocides resistant bacteria from dental unit water line biofilms. | six biocides resistant isolates were isolated from dental unit water lines (duwl) in pakistan. all isolates could tolerate 150 microg ml(-1 )of biocides (5.25% sodium hypocholrite, 35% h2o2, 4% tween 20, 1% povidine iodine, 0.2% chlorohexidine gluconate, 1% ethylene di-amino tetra acetic acid and 1% phenol) on l-agar and 100 microg ml(-1 )in l-broth. the growth rate of all isolates was determined by generating growth curves at 37 degrees c for 48 h. the isolates were found to differ in growth ra ... | 2009 | 19219903 |
| characterization of novel plant growth promoting endophytic bacterium achromobacter xylosoxidans from wheat plant. | nine diazotrophic bacteria were isolated from surface-sterilized roots and culms of wheat variety malviya-234, which is grown with very low or no inputs of nitrogen fertilizer. out of the nine bacteria, four showed indole acetic acid (iaa) production, and five were positive for p solubilization. one isolate, wm234c-3, showed appreciable level of nitrogenase activity, iaa production, and p solubilization ability, and was further characterized with a view to exploiting its plant growth promoting a ... | 2009 | 19224271 |
| [microbiological diagnosis of bronchopulmonary colonization-infection in cystic fibrosis]. | cystic fibrosis (cf), a condition produced by mutations in the gene that encodes the cystic fibrosis transmembrane conductance regulator, is the most prevalent autosomal-recessive hereditary disease in caucasian populations. among other repercussions, this defect leads to an alteration of respiratory secretions and determines a predisposition for chronic bronchopulmonary colonization-infection, which is the main driver of the high morbidity and early mortality of cf patients. colonization by sta ... | 2009 | 19232782 |
| biodegradation and detoxification of endosulfan in aqueous medium and soil by achromobacter xylosoxidans strain cs5. | achromobacter xylosoxidans cs5, capable of utilizing endosulfan as the sole carbon, sulfur and energy source, was isolated from the activated sludge. degradation of endosulfan by strain cs5 was examined by hplc. analysis of culture ph, cells growth, and residual endosulfan demonstrated that cs5 could degrade more than 24.8 mg/l alpha-endosulfan and 10.5mg/l beta-endosulfan after 8 days in aqueous medium, with the formation of endosulfan diol and endosulfan ether as the major metabolites. cell-fr ... | 2009 | 19237238 |
| low rates of pseudomonas aeruginosa misidentification in isolates from cystic fibrosis patients. | pseudomonas aeruginosa is an important cause of pulmonary infection in cystic fibrosis (cf). its correct identification ensures effective patient management and infection control strategies. however, little is known about how often cf sputum isolates are falsely identified as p. aeruginosa. we used p. aeruginosa-specific duplex real-time pcr assays to determine if 2,267 p. aeruginosa sputum isolates from 561 cf patients were correctly identified by 17 australian clinical microbiology laboratorie ... | 2009 | 19261796 |
| achromobacter xylosoxidans, an emerging pathogen in catheter-related infection in dialysis population causing prosthetic valve endocarditis: a case report and review of literature. | dialysis catheter-related infection is a major cause of morbidity and mortality in patients on dialysis. in recent years, there have been reported cases of infections with opportunistic environmental organism, achromobacter xylosoxidans (ax) causing bacteremia in patients on dialysis. however, no previous such reports on prosthetic valve endocarditis in a dialysis patient with achromobacter xylosoxidans were found after a medline search. we report such a case and review the literature. | 2009 | 19281752 |
| novel gene clusters involved in arsenite oxidation and resistance in two arsenite oxidizers: achromobacter sp. sy8 and pseudomonas sp. ts44. | this study describes three gene clusters involved in arsenic redox transformation of two arsenite oxidizers: achromobacter sp. sy8 and pseudomonas sp. ts44. a 17.5-kb sequence containing the arsenite oxidase (aox) gene cluster (aoxx-aoxs-aoxr and aoxa-aoxb-aoxc-aoxd) was isolated from sy8 using a fosmid library approach. similarly, a 14.6-kb sequence including the aox cluster (arsd-arsa-aoxa-aoxb) and the arsenic resistance (ars) gene cluster (arsc1-arsr-arsc2-acr3-arsh-dual specificity phosphat ... | 2009 | 19283378 |
| a tn5051-like mer-containing transposon identified in a heavy metal tolerant strain achromobacter sp. ao22. | achromobacter sp. ao22 (formerly alcaligenes sp. ao22), a bacterial strain isolated from a lead-contaminated industrial site in australia, was previously found to be resistant to moderate to high levels of mercury, copper and other heavy metals. however, the nature and location of the genetic basis for mercuric ion resistance in this strain, had not been previously identified. | 2009 | 19284535 |
| epidemiological typing of clinical isolates of achromobacter xylosoxidans: comparison of phenotypic and genotypic methods. | the purpose of this paper is to evaluate the utility of different typing methods for achromobacter xylosoxidans clinical isolates. ninety-two blood culture isolates of a. xylosoxidans subsp. xylosoxidans were collected over a 25-month period. the typeability, discriminatory power and reproducibility of commonly used phenotypic and genotypic methods, such as resistotyping, plasmid profiling, whole-cell protein fingerprinting, random amplification of polymorphic dna (rapd) and pulsed-field gel ele ... | 2009 | 19373499 |
| in this issue: proteomics 8/2009. | in this issue of proteomics you will find the following highlighted articles:are you sick or are you just getting old: does it matter?we all joke about the hazards of aging: various systems that break down, some you didn't even know you had. but then there's the alternative of not aging. hmmm. now what if aging were a disease? if so, it is worse than any cold i've had. zürbig et al. have found that the proteome of the aging kidney has many markers in common with chronic kidney disease. the degre ... | 2009 | 19382142 |
| bacterial consortium proteomics under 4-chlorosalicylate carbon-limiting conditions. | in this study, the stable consortium composed by pseudomonas reinekei strain mt1 and achromobacter xylosoxidans strain mt3 (cell numbers in proportion 9:1) was under investigation to reveal bacterial interactions that take place under severe nutrient-limiting conditions. the analysis of steady states in continuous cultures was carried out at the proteome, metabolic profile, and population dynamic levels. carbon-limiting studies showed a higher metabolic versatility in the community through upreg ... | 2009 | 19382143 |
| volatile organic compounds: a potential direct long-distance mechanism for antagonistic action of fusarium oxysporum strain msa 35. | fusarium oxysporum msa35 [wild-type (wt) strain] is an antagonistic fusarium that lives in association with a consortium of bacteria belonging to the genera serratia, achromobacter, bacillus and stenotrophomonas in an italian soil suppressive to fusarium wilt. typing experiments and virulence tests provided evidence that the f. oxysporum isolate when cured of the bacterial symbionts [the cured (cu) form], is pathogenic, causing wilt symptoms identical to those caused by f. oxysporum f. sp. lactu ... | 2009 | 19396945 |
| diffusion of hexavalent chromium in chromium-containing slag as affected by microbial detoxification. | an electrochemical method was used to determine the diffusion coefficient of chromium(vi) in chromium-containing slag. a slag plate was prepared from the original slag or the detoxified slag by achromobacter sp. ch-1. the results revealed that the apparent diffusion coefficient of cr(vi) was 4.4 x 10(-9)m(2)s(-1) in original slag and 2.62 x 10(-8)m(2)s(-1) in detoxified slag. the results implied that detoxification of chromium-containing slag by achromobacter sp. ch-1 could enhance cr(vi) releas ... | 2009 | 19446394 |
| [achromobacter xylosoxidans colonization in bronchiectasis]. | | 2010 | 19446928 |
| effects of gassericins a and t, bacteriocins produced by lactobacillus gasseri, with glycine on custard cream preservation. | lactobacillus gasseri la39 and la158 isolated from human-infant feces produce bacteriocins named gassericins a and t, respectively. both gassericins have high heat stability (121 degrees c, 10 min), good ph tolerance (ph 2-11), and strong bactericidality against many gram-positive bacteria, especially lactic acid bacteria, and thus are expected to be effective food preservatives. a microwell plate assay against 12 strains of custard cream spoilage bacteria showed that the gassericins had broader ... | 2009 | 19447968 |
| [cellulose-utilising bacterial association]. | | 2009 | 19449742 |
| [conjunctivitis due to achromobacter xylosoxidans: case report]. | we report here a case of conjunctivitis in an immunocompetent patient due to achromobacter xylosoxidans, which was associated with the use of rigid contact lenses. the bacteria were isolated from the scraped conjunctival swab as well as from the lens cleaning fluid. a. xylosoxidans is an opportunistic pathogen, especially in immunocompromised patients; however, in isolates of ocular infections, from immunocompetent patients, it may be confused with other gram-negative organisms, particularly pse ... | 2009 | 19466343 |
| microbial responses to mustard gas dumped in the baltic sea. | microbiological studies were carried out on chemical weapon dump sites in the baltic sea. the effect of mustard gas hydrolysis products (mghps) on marine microbiota and the ability of microorganisms to degrade mghps were studied. many stations at the dump sites demonstrated reduced microbial diversity, and increased growth of species able to use mustard gas hydrolysis products as sole source of carbon. significant amounts of mghp-degrading bacteria were revealed in the near-bottom water. the mgh ... | 2009 | 19481794 |
| tetracycline and chloramphenicol efficiency against selected biofilm forming bacteria. | despite the constantly increasing need for new antimicrobial agents, antibiotic drug discovery and development seem to have greatly decelerated in recent years. presented with the significant problem of advancing antimicrobial resistance, the global scientific community has attempted to find alternative solutions; one of the most promising ones is the evaluation and use of old antibiotic compounds. a number of old antibiotic compounds, such as aminoglycosides, chloramphenicol, and tetracycline, ... | 2009 | 19484302 |
| identification of phosphorylated and glycosylated sites in peptides by chemically targeted proteolysis. | a chemical strategy has been developed for identifying phosphorylated and glycosylated sites in peptides. phosphoserine, phosphothreonine, o-glycosylserine, and o-glycosylthreonine residues in the peptides were converted to the protease-sensitive s-2-aminoethylcysteine derivatives by beta-elimination followed by michael addition of 2-aminoethanethiol.the resultant lysine analogs were cleaved with achromobacter lysine endopeptidase.the predicted proteolytic fragments were analyzed by mass spectro ... | 2002 | 19498988 |
| multiple drug-resistant alcaligenes xylosoxidans keratitis in a sanitation worker. | to report a case of daily wear soft contact lens-associated keratitis caused by alcaligenes xylosoxidans in a portable toilet sanitation worker. | 2009 | 19516143 |
| antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy. | this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ... | 2009 | 19567344 |
| broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis. | broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples. | 2009 | 19570210 |
| cough-generated aerosols of pseudomonas aeruginosa and other gram-negative bacteria from patients with cystic fibrosis. | pseudomonas aeruginosa is the most common bacterial pathogen in patients with cystic fibrosis (cf). current infection control guidelines aim to prevent transmission via contact and respiratory droplet routes and do not consider the possibility of airborne transmission. it was hypothesised that subjects with cf produce viable respirable bacterial aerosols with coughing. | 2009 | 19574243 |
| association between contaminated faucets and colonization or infection by nonfermenting gram-negative bacteria in intensive care units in taiwan. | this study was designed to determine the strength of the association between the isolation of nonfermentative gram-negative bacilli (nfgnb) from tap water faucet aerators and the prevalence of colonization or infection of patients in intensive care units (icus). surveillance cultures were obtained during a 4-month period from 162 faucet aerators located in seven different icus. the prevalence of colonization or infection of icu patients with nfgnb was determined by prospective surveillance durin ... | 2009 | 19587299 |
| complicated intra-abdominal infection caused by extended drug-resistant achromobacter xylosoxidans. | achromobacter xylosoxidans (formerly alcaligenes xylosoxidans) is a rare but important nosocomial pathogen. antibiotic resistance has been increasing during the past decade. a. xylosoxidans may be confused with pseudomonas spp. but, unlike pseudomonas spp., this organism has peritrichous flagella. complicated intra-abdominal infection with a. xylosoxidans has rarely been reported in the literature. this report is of an immunocompetent patient with acute cholecystitis complicated by an intra-abdo ... | 2009 | 19597652 |
| dna-stable isotope probing integrated with metagenomics for retrieval of biphenyl dioxygenase genes from polychlorinated biphenyl-contaminated river sediment. | stable isotope probing with [(13)c]biphenyl was used to explore the genetic properties of indigenous bacteria able to grow on biphenyl in pcb-contaminated river raisin sediment. a bacterial 16s rrna gene clone library generated from [(13)c]dna after a 14-day incubation with [(13)c]biphenyl revealed the dominant organisms to be members of the genera achromobacter and pseudomonas. a library built from pcr amplification of genes for aromatic-ring-hydroxylating dioxygenases from the [(13)c]dna fract ... | 2009 | 19648381 |
| parapusillimonas granuli gen. nov., sp. nov., isolated from granules from a wastewater-treatment bioreactor. | a novel betaproteobacterium, designated strain ch07(t), was isolated from granules from the wastewater-treatment bioreactor of an alcohol fermentation factory in south korea. in order to determine its taxonomic position, the novel strain was characterized using a polyphasic approach. the new strain was gram-negative, facultatively anaerobic, non-spore-forming, motile and short rod-shaped. 16s rrna gene sequence analysis revealed that strain ch07(t) belonged to the class betaproteobacteria, being ... | 2010 | 19671719 |
| biphenyl-metabolizing bacteria in the rhizosphere of horseradish and bulk soil contaminated by polychlorinated biphenyls as revealed by stable isotope probing. | dna-based stable isotope probing in combination with terminal restriction fragment length polymorphism was used in order to identify members of the microbial community that metabolize biphenyl in the rhizosphere of horseradish (armoracia rusticana) cultivated in soil contaminated with polychlorinated biphenyls (pcbs) compared to members of the microbial community in initial, uncultivated bulk soil. on the basis of early and recurrent detection of their 16s rrna genes in clone libraries construct ... | 2009 | 19700551 |
| nitrite reductase genes in halobenzoate degrading denitrifying bacteria. | abstract diversity of the functional genes encoding dissimilatory nitrite reductase was investigated for the first time in denitrifying halobenzoate degrading bacteria and in two 4-chlorobenzoate degrading denitrifying consortia. nitrite reductase genes were pcr-amplified with degenerate primers (specific to the two different types of respiratory nitrite reductase, nirs and nirk), cloned and sequenced to determine which type of nitrite reductase was present in each isolate and consortium. halobe ... | 2003 | 19719666 |