Publications

TitleAbstractYear
Filter
PMID
Filter
[rapid detection of campylobacter jejuni by enzyme linked immunosorbent assay in the patients with acute enteritis (i)]. 19863712788
effect of iron concentration on toxin production in campylobacter jejuni and campylobacter coli.the effect of iron concentrations in culture media on supernatant yields of campylobacter cytotonic toxin (cct) was studied. of the 118 campylobacter spp. strains surveyed, 78.8% produced toxin in brucella broth or in casamino acids--yeast extract (cye) broth. when the iron concentration of cye was increased from 0.44 microgram/ml (7.9 microm) to 0.65 microgram/ml (11.6 microm) by the addition of ferric chloride, 94.9% of the strains were positive for toxin in a ganglioside gm1 based, enzyme-lin ...19863719459
[serotyping of campylobacter jejuni/coli]. 19863719684
campylobacter jejuni bacteraemia as a cause of recurrent fever in a patient with hypogammaglobulinaemia.in a hypogammaglobulinaemic patient with faecal cultures persistently positive for campylobacter jejuni it was shown that c. jejuni bacteraemia was responsible for a long history of self-limiting attacks of fever. a focus of infection outside the gastro-intestinal tract was not found. antimicrobial treatment failed to eradicate c. jejuni. antibodies against c. jejuni were not detectable and there was also a defect in serum bactericidal activity. in contrast with normal serum, it was shown that, ...19863722839
campylobacter jejuni appendicitis. 19863724634
effect of egg storage upon the survival of campylobacter jejuni in laboratory-infected fertile poultry eggs.fertile eggs were infected by campylobacter jejuni in the laboratory by a temperature differential method of inoculation, which resulted in up to 10% of the hatched birds carrying c. jejuni in the intestine. when infected eggs were stored for 5 1/2 days before incubation, the infection rate of the eggs had decreased to 20% or less when set, and no infected chicks were hatched. inoculation of eggs after 8 days in storage also failed to yield infected chicks. in all cases, the hatch ratio was no d ...19863729872
campylobacter jejuni associated with a perirectal abscess. 19863730961
colonization and infection of athymic and euthymic germfree mice by campylobacter jejuni and campylobacter fetus subsp. fetus.human clinical strains of campylobacter jejuni and campylobacter fetus subsp. fetus colonized the gastrointestinal tracts of both athymic (nu/nu) and euthymic (+/nu) germfree mice (balb/c). viable campylobacter spp. (10(9) to 10(10) cfu/g [dry weight] of cecum and colon contents) were isolated on day 3 after oral challenge, and similar large numbers of viable cells were evident at several intervals during a 10-month experiment. the stomachs and upper small intestines of nu/nu and +/nu mice that ...19863733222
monoclonal antibodies directed against the flagella of campylobacter jejuni: cross-reacting and serotypic specificity and potential use in diagnosis.nine monoclonal antibodies directed against the flagella of campylobacter jejuni strain 81116 have been investigated for serotypic and cross-reacting activity using a panel of 17 penner serotype strains of c. jejuni. four monoclonal antibodies were exclusively specific for serotype-6 strains, which was the serotype of c. jejuni strain 81116. two monoclonal antibodies cross-reacted with all the flagellated strains of c. jejuni tested. one of these cross-reacting monoclonal antibodies, cf5, was fo ...19863734423
natural campylobacter colonization in chickens raised under different environmental conditions.a cross-sectional study of 447 laying hens (age range 0-65 weeks) and a longitudinal study of 164 similar birds showed that campylobacter jejuni was not present in the faeces of newly hatched chicks, but that colonization arose after 5-9 weeks. a survey of 250 broilers obtained from four breeders showed that all were negative for c. jejuni before and after slaughter at the age of 5 weeks. once c. jejuni had appeared in a flock, it rapidly spread to virtually all birds, but at the age of 42 weeks ...19863734424
aetiology and epidemiology of acute gastro-enteritis in swedish children.in a prospective 1-year study, 144 children attending or admitted to hospital and 272 children outside hospital with acute gastro-enteritis and 200 controls were investigated by a broad panel of diagnostic methods for enteropathogenic agents in the faeces and for related antibody responses. enteropathogens were identified in 77% of the inpatients, 63% of the outpatients and 8% of the controls. rotavirus and yersinia enterocolitica were detected significantly more often among inpatients. altogeth ...19863734469
distribution of campylobacter jejuni in wild birds and serogroup of isolates by slide agglutination technique. 19863735880
the nucleotide sequences of two trna genes from campylobacter jejuni. 19863737410
isolation of campylobacter jejuni from intestinal contents of chickens in mexico city. 19863738283
[characteristics of the susceptibility to antimicrobial agents of campylobacter jejuni/coli strains]. 19863738362
campylobacter jejuni in hospitalized children with diarrhoea in chiang mai, thailand.in this hospital based study, campylobacter jejuni, although endemic in northern thailand was not found in a significantly higher percentage of diarrhoea than in control children. it was isolated from the stools of 14 of 208 diarrhoea (6.7%) and 6 of 108 (5.5%) control patients. ten of the 14 positive diarrhoea cases were mixed infections, other pathogens isolated simultaneously were enteropathogenic e. coli 4x, shigella spp. 3x, salmonella spp. 4x, rotavirus 3x, plesiomonas shigelloides 1x and ...19863738608
campylobacter colitis in ranch mink in ontario.outbreaks of colitis, where campylobacter jejuni and campylobacter coli were the only pathogens isolated occurred in weanling mink (mustella vision) on two commercial mink ranches in ontario. lesions were restricted to the proximal colon and were characterized by multiple 1 mm focal or 1 mm linear erosions/ulcers in the region 2 cm distal to the ileal-colonic junction. histological changes included thickening of the colonic mucosa, inflammatory cell infiltrate in the lamina propria and submucosa ...19863742356
use of skirrow medium with membrane filters for selective isolation of campylobacter jejuni from stools. 19863743564
the prevalence of bacterial intestinal pathogens in a healthy rural population in southern india.in a one-year prospective survey bacterial intestinal pathogens unassociated with diarrhoeal episodes were isolated from 20.5% of stool samples from 48.5% of a stratified random sample of the population of a village in southern india. campylobacter jejuni was the pathogen most frequently isolated, followed by enteropathogenic serotypes of escherichia coli. the incidence of diarrhoea in the study population was lower than the frequency of isolation of bacterial intestinal pathogens. it is necessa ...19863746876
[rapid detection of anti-campylobacter jejuni antibodies by enzyme linked immunosorbent assay in the patients with acute enteritis. (ii)]. 19863747201
preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.short synthetic oligonucleotides have been covalently cross-linked to alkaline phosphatase using the homobifunctional reagent disuccinimidyl suberate. the oligomers, twenty-one to twenty-six bases in length, are complementary to unique sequences found in herpes simplex virus, hepatitis b virus, campylobacter jejuni and enterotoxigenic escherichia coli. each oligomer contains a single modified base with a 12-atom "linker arm" terminating in a reactive primary amine. cross-linking through this ami ...19863748805
campylobacter infection associated with raw milk. an outbreak of gastroenteritis due to campylobacter jejuni and thermotolerant campylobacter fetus subsp fetus.raw milk is identified with increasing numbers of outbreaks of gastroenteritis and is an important vehicle for transmission of campylobacter infection. unlike campylobacter jejuni, campylobacter fetus subsp fetus has not been associated with common-source outbreaks of gastroenteritis. this report describes an outbreak of gastroenteritis involving c jejuni and a thermotolerant strain of c fetus subsp fetus associated with raw milk. fifteen (39%) of 38 persons who attended a banquet in wisconsin i ...19863753617
strain characterization and grouping of campylobacter jejuni and campylobacter coli by interaction with lectins.strains of campylobacter jejuni and campylobacter coli were characterized and grouped by their distinct reaction patterns with lectins. heating of the campylobacter cultures to 100 degrees c and holding for 30 to 60 min greatly enhanced their reactivity with lectins and permitted the grouping of all but 3 of 155 cultures tested in this study without interference of autoagglutination and other nonspecific activities. the lectin reaction patterns of the heated cultures were stable and reproducible ...19863754264
[diseases caused by campylobacter jejuni in childhood]. 19863757003
the chicken embryo as a model for campylobacter invasion: comparative virulence of human isolates of campylobacter jejuni and campylobacter coli.eleven-day-old chicken embryos were used to compare the relative virulence of minimally passaged human isolates of campylobacter jejuni and campylobacter coli. graded doses of bacteria were inoculated onto the chorioallantoic membrane, and 50% lethal doses were calculated at 72 h postinfection. strains varied markedly in their ability to invade the chorioallantoic membrane and kill the embryos. the 50% lethal doses varied by about 6 logs for 25 strains of c. jejuni, and by 2 logs for 5 strains o ...19863759232
chronic diarrhea associated with campylobacter jejuni infection in a cat.campylobacter jejuni was isolated from a cat with chronic diarrhea. the diarrheic cat and another cat (which previously had diarrhea) in the same household had bactericidal antibody titers to the c jejuni. clinical response to antibiotic therapy and not recovering campylobacter sp from normal feces after treatment also supported the diagnosis of campylobacter-associated diarrhea. although the owner had a protracted episode of diarrhea, c jejuni was not isolated from the owner's feces, nor was a ...19863759618
comparative efficacy of seven selective media for isolating campylobacter jejuni.diarrheal stools from 263 patients were inoculated on seven selective media: butzler selective medium, blaser medium, skirrow blood agar, preston campylobacter selective medium, preston campylobacter blood-free medium, butzler virion medium, and modified preston medium (with amphotericin b [2 mg/liter]). a similar number of campylobacter jejuni strains were isolated from all the media studied; nevertheless, the presence of competing fecal flora (ff) made the detection of suspect colonies difficu ...19863760137
[campylobacter jejuni enteritis in infants]. 19863764182
[acute campylobacter jejuni enterocolitis in children under 2 years of age]. 19863764191
[campylobacter jejuni septicemia]. 19863764855
viable but nonculturable stage of campylobacter jejuni and its role in survival in the natural aquatic environment.conditions influencing the survival of campylobacter jejuni in the natural aquatic environment have been determined. release of campylobacter spp. into natural waters by animal hosts is postulated to play a key role in the maintenance of viability and transmission of the organism in the environment. laboratory flask microcosms containing filter-sterilized stream water were used to test c. jejuni for the ability to remain viable in simulated natural systems. the microcosms were compared with the ...19863767358
[investigation on serotyping of the campylobacter jejuni group]. 19863769671
association with hela cells of campylobacter jejuni and campylobacter coli isolated from human feces.we developed a rapid in vitro test for determining the association of campylobacter jejuni and c. coli with hela cells. association was expressed as a weighted mean of the number of bacteria associated with one cell in an association index (ai). the reproducibility of the ai was checked by repeating the test six times, using four strains chosen at random. means and standard deviations of the means were 7.3 +/- 1.2, 6.8 +/- 0.9, 1.8 +/- 1.2, and 0.1 +/- 0.2. the experimental conditions for which ...19863770943
techniques for the optimum recovery of cold injured campylobacter jejuni from milk or water.when broth was inoculated with cells of campylobacter jejuni that had been injured by chilling there was a fall in the viable population of up to 90%. it was greater at 43 degrees than 37 degrees c and in the presence of certain antibiotics and in some cases resulted in a surviving population that was below the minimum inoculum for subsequent growth. a technique of pre-enrichment in non-selective culture broth at 37 degrees c for 2 h before the addition of antibiotics and incubation at 43 degree ...19863771411
modified selective medium for isolation of campylobacter spp. from feces: comparison with preston medium, a blood-free medium, and a filtration system.our previously described (h. goossens, m. de boeck, and j. p. butzler, eur. j. clin. microbiol. 2:389-393, 1983) selective medium, consisting of cefoperazone (15 mg/liter), rifampin (10 mg/liter), colistin (10,000 iu/liter), and amphotericin b (2 mg/liter) (medium m1), for the isolation of campylobacter jejuni and campylobacter coli from stool specimens was modified as follows: cefoperazone (30 mg/liter), rifampin (10 mg/liter), and amphotericin b (2 mg/liter) (medium m2). a comparative study of ...19863771769
biotype and serogroup distribution of campylobacter isolates from children in nigeria.a total of 101 campylobacter isolates from nigerian children with or without gastroenteritis were biotyped and serogrouped by using the lior typing schemes (h. lior, j. clin. microbiol. 20:636-640, 1984; h. lior, d. l. woodward, j. a. edgar, l. j. laroche, and p. gill, j. clin. microbiol. 15:761-768, 1982). fifty-three (52.5%) of the isolates were campylobacter jejuni biotype i, 29 (28.7%) were c. jejuni biotype ii, 10 (9.9%) were campylobacter coli biotype i, and 9 (8.9%) were c. coli biotype i ...19863771772
rate of campylobacter spp. isolation in three regions of ontario, canada, from 1978 to 1985.isolation rates of campylobacter spp. were analyzed for an 8-year period, 1978 through 1985. three laboratories of the ontario ministry of health examined 146,842 human feces samples for bacterial pathogens, including campylobacter jejuni and campylobacter coli. campylobacter spp. were isolated from 5,580 specimens (3.8%), with monthly isolation rates ranging from 1.1 to 7.4%. the data showed a seasonal distribution of isolations, with peaks during the summer months (june to september). most inf ...19863771774
monosaccharide composition of lipopolysaccharides from campylobacter jejuni and campylobacter coli.the monosaccharide composition of the lps from 5 campylobacter jejuni strains and 7 campylobacter coli strains has been studied. all lps's contained kdo, heptose, glucosamine, glucose, and (with one exception) galactose. all c. jejuni and 3 c. coli lps's contained greater than 1% galactosamine. 3-amino-3.6-dideoxyglucose was present in all but one c. coli lps and in only one c. jejuni lps.19863772734
campylobacter bacteraemia: a report of 10 cases.ten cases of campylobacter bacteraemia, which were diagnosed over a three-year period, are described. four patients presented with acute gastrointestinal illness, three patients had acute febrile illnesses, two patients had cellulitis and one patient presented with ureteric colic. two patients had lifelong agammaglobulinaemia, two were undergoing corticosteroid therapy and there was one nosocomial infection. in contrast with previous reports, the most common species that was isolated was campylo ...19863773807
effect of ascorbic, isoascorbic and dehydroascorbic acids on the growth and survival of campylobacter jejuni.ascorbic acid (asa), added to nutrient broth at a concentration of 5 mmol/l, was bactericidal towards campylobacter jejuni grown at 42 degrees c in a micro-aerobic atmosphere. specific enzymes, radical scavengers, metal chelators and reducing agents were tested as possible antagonists to the cytotoxicity of asa. the addition of catalase or of the metal chelators ceruloplasmin or desferal did not prevent the cytotoxic effect of asa. the addition of the hydroxyl radical scavengers mannitol, format ...19863781941
faecal carriage rate of yersinia species.a total of 1,203 unselected routine faecal samples from 1,006 patients were cultured for yersinia species by a cold enrichment technique. seventy-five specimens (6.1%) from 63 patients were culture-positive for yersinia spp. fifty-two were yersinia enterocolitica, 22 yersinia frederiksenii and 1 yersinia intermedia. the predominant y. enterocolitica isolates belonged to biotype 1 - serotype 0:6, 30 or serotype 0:5, 27. y. frederiksenii strains were non-typable. forty isolates were recovered from ...19863782782
cytotoxic and enterotoxic activities of campylobacter jejuni are not specified by tetracycline resistance plasmids pmak175 and pua466.the 45-kilobase tetracycline resistance plasmids pmak175 and pua466 from campylobacter jejuni were examined using curing and mating experiments. however, these plasmids encoded neither cytotoxin production, as determined in vero cells, nor enterotoxin activity, as determined in chinese hamster ovary cells.19873793867
evaluation of four enrichment media for isolation of campylobacter jejuni.diarrheal stool specimens were inoculated into the following media: alkaline peptone water (apw), bruce-zochowsky medium (bz), campylobacter enrichment broth (ceb), campy-thio broth (ct), and skirrow blood-agar (sk) plate. all media were incubated at 42 degrees c in microaerophilic conditions for 24 h. afterwards, a new sk plate was inoculated from every liquid medium. campylobacter jejuni was isolated from 43 of the 259 specimens when ct was used, from 45 when apw was used, from 46 when bz was ...19873793873
the relative importance of various enteropathogens as a cause of diarrhoea in hospitalized children in chiang mai, thailand.a study of 208 children aged under 15 hospitalized with diarrhea and 108 matched controls was conducted at chiang mai university hospital, chiang mai, thailand from may 1983 to april 1984 to investigate the incidence, seasonal distribution, and causative agents of diarrhea. 1 or more enteropathogens were isolated from 121 (58%) diarrhea patients and from 32 (30%) controls. in diarrhea patients, shigella spp. were most frequently isolated (22%) followed by enteropathogenic escherichia coli (epe ...19863794260
low incidence of campylobacter enteritis in northern ireland.in a prospective survey carried out over 9 months in 1984 in the department of bacteriology, belfast city hospital, campylobacter jejuni was isolated from 24 out of 1200 faecal specimens (2%) from patients with acute diarrhoea. this isolation rate is much lower than that from other parts of mainland britain which report an isolation rate of between 8 and 15%. it is difficult to explain this large discrepancy but the limited availability of unpasteurized milk and the generally worse summer here ( ...19863794324
phagocytosis of campylobacter jejuni and c. coli by peritoneal macrophages.guinea-pig resident peritoneal macrophages had no activity against freshly isolated campylobacter jejuni, whilst c. coli was phagocytosed and killed. the number of bacteria killed by macrophages always exceeded the number of those ingested, suggesting an extracellular mechanism of killing.19863794651
early treatment with erythromycin for campylobacter jejuni-associated diarrhea. 19873794884
campylobacter jejuni infections. 19873796409
the viability of campylobacter jejuni on refrigerated chicken drumsticks.radappertized chicken drumsticks were experimentally contaminated with suspensions of campylobacter jejuni in two trials. qualitative analysis on drumsticks with an initial level of contamination of 4.8 x 10(3) cfu/cm2 showed that viability was retained for at least 10 days of storage at either 9 degrees or -12 degrees c. in a second quantitative trial, the level of contamination declined from 9.9 x 10(2) cfu/cm2 to 4.5 x 10(1) cfu/cm2 after 7 days at -20 degrees c. thereafter, c. jejuni persist ...19863798737
campylobacter jejuni contamination of eggs.contamination of commercial table eggs with a fecal suspension containing 4.4 x 10(6) cfu/g campylobacter jejuni resulted in shell penetration in 3/70 eggs and recovery of the organism from homogenized egg contents in 1/70 eggs. viability of c. jejuni on the shell surface was retained for only 16 hours, attributed to desiccation of the fecal suspension. a field survey of three commercial laying farms and their associated egg-packing plants showed that hens demonstrated to be fecal shedders of c. ...19863798738
detection of serum antibody response in patients infected with one strain of campylobacter jejuni with a dig-elisa method.paired sera from 10 patients and a convalescence sample from one patient suffering from campylobacteriosis were analysed for igg, igm and iga antibodies against the infective organism (campylobacter jejuni pen 0:6,7) with a dig-elisa system. either formalinized, ethanol-inactivated or heat-inactivated preparations of the infecting organism were used as antigens. cross reactivity was tested with human sera having agglutinating antibodies against yersinia enterocolitica (n = 6) or salmonella typhi ...19863808455
[septicemia caused by campylobacter jejuni]. 19863810578
treatment of campylobacter gastroenteritis.twin boys suffered from recurrent diarrhoea due to campylobacter jejuni after entering a day nursery. stool sampling of all 17 children at the nursery revealed c. jejuni in 12. simultaneous treatment with antibiotics of all children with positive cultures successfully eradicated the infection.19873813645
[campylobacter jejuni/coli in partridges (perdix perdix l.) and pheasants (phasianus colchicus l.)]. 19863814066
demonstration of a flagellar antigen shared by a diverse group of spiral-shaped bacteria that colonize intestinal mucus.western blot analysis showed that there is little immunological cross-reactivity of the human pathogens campylobacter jejuni and campylobacter coli with campylobacter pyloridis, except for a very strong cross-reaction between the flagellins. this same antigenic cross-reaction was found with two isolates of gram-negative spiral microaerophilic bacteria that are known to colonize the intestinal mucosa of rodents, but not with the flagellins of a number of other motile bacteria. it is proposed that ...19873818099
[epidemiology and epizootiology of enteritis caused by campylobacter jejuni and campylobacter coli]. 19863822876
[incidence of campylobacter jejuni/coli in swine and poultry. possible relationship to human disease on the basis of characteristics of species and serotype]. 19863823733
[frequency of infection with campylobacter jejuni/coli and the occurrence of igg, igm and complement-fixing antibodies against the agent in workers in poultry abattoirs in santiago, chile]. 19863825343
campylobacter jejuni infection in the ferret: an animal model of human campylobacteriosis.campylobacter infection in weanling ferrets (mustela putorius furo) was studied as an animal model for enteric campylobacteriosis in persons. the screening of fecal cultures on selective campylobacter media showed that campylobacter jejuni/coli was not present in the normal enteric flora. intragastric feeding of a mixture of cat feed and 2.5 x 10(8) c jejuni isolated from ferrets with naturally occurring proliferative colitis was accomplished. all ferrets (n = 8) became infected on 3 days after ...19873826848
campylobacter jejuni associated with acute relapse and abscess formation in crohn's disease. 19853831348
[antibiotypes of campylobacter jejuni strains of human and animal origin]. 19853832256
[intestinal campylobacter jejuni infection in chilean infants]. 19853834562
in vitro susceptibilities of campylobacter-like organisms to twenty antimicrobial agents.we determined mics of 20 antimicrobial agents for 50 representative strains of four subgroups of campylobacter-like organisms (clos) by agar dilution. ampicillin, gentamicin, doxycycline, tetracycline, ceftriaxone, rifampin, spectinomycin, nalidixic acid, and chloramphenicol were active against all strains of clos. most clo strains (83%) were inhibited by 4 micrograms of sulfamethoxazole per ml and by 8 micrograms of trimethoprim-sulfamethoxazole per ml. of type 1 strains, 28% were resistant to ...19853834828
[symptomatic and asymptomatic infections due to campylobacter jejuni]. 19853836455
[electron microscope observation of campylobacter jejuni]. 19853837358
[isolation and identification of campylobacter jejuni from chicken flocks]. 19853837359
medical problems of the homosexual adolescent.physicians treating adolescents should take a complete sexual history, including sexual orientation and practices, to determine whether their patients are homosexually active. lesbians are at very low risk for sexually transmitted diseases, but they do have other health concerns. four general groups of conditions may be encountered in homosexually active men: classical sexually transmitted diseases (gonorrhea, infections with chlamydia trachomatis, syphilis, herpes simplex infections, genital wa ...19853839219
[the behavior of campylobacter jejuni in various foodstuffs].for the purpose of ascertaining the survival time of campylobacter jejuni in foodstuffs under differing conditions, milk, ice-cream, meat salad and drinking water were inoculated with a defined quantity of campylobacter jejuni and subsequently the number of colony-forming units was determined at first at 12-hour intervals and later once a day. it was found that the survival time in milk at +5 degrees c was 15 days, in drinking water 6 days, but only 7 and 4 days respectively at 22 degrees c. in ...19853839617
[campylobacter jejuni enteric infections in rosario]. 19853842471
comparative in vitro activities of twelve antimicrobial agents against campylobacter species.the in vitro susceptibility of 27 campylobacter jejuni, 31 campylobacter coli, and 30 campylobacter fetus subsp. fetus strains to 12 antimicrobial agents was determined. ciprofloxacin, a new quinoline derivative, was the most active agent tested. antimicrobial susceptibility differed among the three species tested.19853873216
campylobacter enteritis and bloody stools in the neonate.within 72 hours of birth three babies had loose stools containing fresh blood, mucus, and campylobacter jejuni/coli. campylobacter enteritis should be considered in newborn babies passing blood per rectum.19853874603
prevalence of campylobacter jejuni in felidae in baton rouge, louisiana.a prevalence study revealed campylobacter jejuni in rectal swabs of 1% of a sample of 430 domestic cats and from one species of 15 zoo felines which were examined. there was no correlation between age, presence of diarrhea, source of sample (animal shelter, veterinary clinic, domestic pet) and the isolation of c. jejuni. a controlled exposure trial confirmed that fecal shedders can transmit infection to susceptible contacts which subsequently demonstrate transient diarrhea. infected cats show a ...19853877024
clinical and laboratory features of yersinia, campylobacter and salmonella infections in children.clinical and laboratory findings of 78 paediatric patients with infections due to enteroinvasive bacteria were analysed. the material included 33 cases of yersinia enterocolitica, 21 of campylobacter jejuni, 21 of salmonella and 2 of shigella infection, reflecting the current order of frequency ot these enteropathogens in finnish paediatric population. diarrhoea was the presenting symptom in 73% of the cases with yersinia, 90% with campylobacter and 100% with salmonella. conversely, abdominal pa ...19853883048
isolation, characterization, and serotyping of campylobacter jejuni and campylobacter coli from slaughter cattle.a total of 525 specimens from 100 slaughter beef cattle were examined for the presence of campylobacter jejuni and campylobacter coli by direct plating and enrichment techniques. isolates were identified by cultural, biochemical, antibiotic sensitivity, and immunofluorescence tests and further characterized with the aid of recently developed biotyping and serotyping methods. fifty animals were positive for c. jejuni; only one was positive for c. coli. the distribution pattern of c. jejuni-positi ...19853888109
[epidemiology and clinical aspects of campylobacter enteritis in childhood].stool specimens of 478 children with enteritic symptoms were screened for enteropathogenic bacteria over a 5-month period. 28 cases of infection due to campylobacter jejuni were found. the incidence of campylobacter enteritis exceeded that of salmonella and shigella infections recorded over the same period (17 and 11 cases, respectively). infected children-aged between 2 months and 15 1/2 years-presented with symptoms of mild gastroenteritis. 18 patients had bloody diarrhoea, whilst 4 children a ...19853890373
[occurrence of campylobacter jejuni in animals and its significance for the human].the frequency of human infections caused by campylobacter (c.) jejuni is thought to be at present as significant as that of infections with salmonella. the epidemiology of human infections with c. jejuni is not well understood, although numerous species of animals are an important reservoir for this microorganism. in an overview the occurrence of c. jejuni at different domestic animals and pets is described. there is also given a report about possible direct and indirect ways for the infections ...19853895567
[campylobacter jejuni infections in children].in a two-year-study, the primary feces of all children with gastroenteritis or acute abdominal symptoms were checked for the presence of campylobacter jejuni (c. j.) even when there was no diarrhea. c. j. could be isolated 19 times from among 974 fecal samples. the most frequent admission diagnosis after gastroenteritis was the suspicion of appendicitis. in the forefront of the clinical picture was abdominal pain, which radiated into the right lower abdomen especially in children of school age, ...19853895753
occurrence of campylobacter jejuni and giardia species in muskrat (ondatra zibethica).a total of 189 muskrat fecal samples were surveyed for campylobacter and giardia species. campylobacter jejuni was recovered from 47.5% of these samples, and giardia species were detected in 82.5%. these findings indicate that muskrat may be of importance to the health both of humans and of domestic animals.19853896141
the occurrence and significance of campylobacter jejuni in man and animals.campylobacter jejuni, which is now recognized as a discrete species, is a gram negative, microaerophilic, thermophilic, nalidixic acid sensitive, hippurate positive pathogen requiring special selective media for propogation. the organism is widely distributed in avian species, experimental and companion animals and in humans. mammalian campylobacteriosis is characterized by an enterocolitis of variable severity. the prevalence of the condition is relatively high in young individuals, in underdev ...19853898555
pathogenic properties of campylobacter jejuni: assay and correlation with clinical manifestations.the pathogenic properties of 20 strains of campylobacter jejuni isolated from persons with clearly defined clinical manifestations were determined. cell-free broth filtrates were examined for (i) enterotoxin production by chinese hamster tissue culture assay and an enzyme-linked immunosorbent assay (elisa) employing gm1 ganglioside and affinity-purified antiserum to escherichia coli heat-labile toxin, (ii) cytotoxin production by vero and hela cell tissue culture lines, and (iii) their ability t ...19853899937
comparison of four hippurate hydrolysis methods for identification of thermophilic campylobacter spp.the test for hippurate hydrolysis is critical for separation of campylobacter jejuni and c. coli strains. glycine and benzoic acid are formed when hippurate is hydrolyzed by c. jejuni. the test used in most laboratories is one of several variations of the ninhydrin tube test described by hwang and ederer (m. hwang and g. m. ederer, j. clin. microbiol. 1:114-115, 1975) for detection of glycine. we evaluated three modifications of the hwang and ederer method and the gas-liquid chromatographic (glc ...19853902875
acute childhood diarrhoea in naples: an aetiologic study.a potential aetiologic agent was detected in the stools of 56% of 118 children hospitalized in naples for acute diarrhoea. rotavirus and salmonella were the agents most commonly associated with disease, accounting for 23 and 17 percent of cases, respectively. campylobacter jejuni, enterotoxigenic escherichia coli, yersinia and shigella were less frequently isolated (total, 11% of cases). these findings fit well with the epidemiological picture described for other developed countries, except for ...19853906366
the impact of routine isolation techniques for campylobacter jejuni in stool cultures.campylobacter jejuni is recognized as a leading cause of bacterial diarrhea. we studied the impact of performing routine isolation techniques for c jejuni in stool cultures in a community hospital. since instituting such techniques, we have found c jejuni to be the leading cause of bacterial diarrhea at our hospital. clinical and laboratory manifestations and treatment did not distinguish patients with c jejuni from patients with salmonella species isolated from the stool. we believe that microb ...19853906909
[campylobacter jejuni infections]. 19853908243
demonstration of antibodies reacting with escherichia coli heat-labile enterotoxin in sera of patients suffering from campylobacter jejuni/coli. 19853909022
in vitro blastogenic response and immunoglobulin synthesis in murine spleen cells stimulated with lipopolysaccharides from campylobacter jejuni and campylobacter coli.lipopolysaccharides isolated from strains of campylobacter jejuni and campylobacter coli were mitogenic for spleen cells as measured by 3h-thymidine incorporation. the incorporation was dose dependent with an increase with increased concentration of lps. addition of lps beyond 500 micrograms/ml gave inhibition of incorporation. stimulation of the spleen cells for 3 days with lps led to a polyclonal activation of immunoglobulin synthesis. the amount of immunoglobulins synthesised showed a maximum ...19853909739
[occurrence and transmission of campylobacter jejuni/coli in young poultry fattening production. 1]. 19853910389
studies on the identification of campylobacter species using biochemical tests and high-performance liquid chromatography.a total of 56 strains of campylobacter jejuni (c. jejuni) isolated from diarrhoeal patients were characterized by biochemical tests. the following reactions were performed: hydrolysis of hippurate, reduction of nitrate and nitrite, activity of deoxyribonuclease, hydrolysis of tween(r) 40, 60 and 80. including the hydrolysis of the different tweens(r), 14 biotypes could be distinguished. 19 out of the 56 strains of c. jejuni and the nomenclatural type strains of c. jejuni, c. coli, c. laridis, c. ...19853911663
expression of campylobacter genes for proline biosynthesis in escherichia coli.cloned dna from campylobacter jejuni was found to complement auxotrophic defects in proline metabolism in several strains of escherichia coli. a 4.4-kilobase fragment of campylobacter dna encodes the genes analogous to the proa and b genes of e. coli and salmonella typhimurium.19853912037
[epidemiology of campylobacter jejuni infection]. 19853915445
microbiological aspects of goat's milk. a public health laboratory service survey.in a 12-month survey (june 1982-may 1983) 41 laboratories examined 2493 samples of goat's milk for colony counts and the presence of pathogens. the statutory tests for cow's milk were also applied. surface counts of less than 10(5) organisms per ml of raw milk were given by 79% of samples at 37 degrees c and by 76% at 22 degrees c. there were less than 100 coliforms per ml in 71% of samples, less than 10 escherichia coli per ml in 91%. staphylococcus aureus was not detected in countable numbers ...19853919086
immunological relationship of the b subunits of campylobacter jejuni and escherichia coli heat-labile enterotoxins.the application of dissociation techniques, involving gel filtration in the presence of guanidine, to a semipurified preparation of campylobacter jejuni heat-labile enterotoxin yielded a material whose functional and immunological properties resemble those of the b subunits of cholera toxin and escherichia coli heat-labile toxin (lt). the c. jejuni toxin b subunit reacted with gm1 ganglioside in an enzyme-linked immunosorbent assay, but lacked the holotoxin's cytotonic activity in the chinese ha ...19853922890
the effect of carbon dioxide packaging on detection of campylobacter jejuni from chicken carcasses.commercially-processed broilers were held at 4 c in carbon dioxide(co2)-flushed bags or in natural atmosphere for nine days prior to determining the most probable number of campylobacter jejuni on individual carcasses. fifty broilers were evaluated. the co2-enriched atmosphere packaging of broilers had no detectable effect on the c. jejuni populations.19853923461
campylobacter jejuni infection in colorado: unexplained excess of cases in males.between january 1, 1981, and december 31, 1982, the colorado department of health received reports of 1,185 culture-confirmed cases of campylobacter jejuni infection. incidence rates were highest among infants less than 1 year old and among persons aged 20-29 years. the distribution of cases by sex showed a predominance among males at all ages except 40-59 years, the most marked predominance occurring in infants under 1 year. the higher rates for males were also significant for all ages combined ...19853923544
[isolation of campylobacter jejuni and campylobacter coli from intestinal contents and feces of chickens]. 19853925041
antibiotic and deoxycholate resistance in campylobacter jejuni following freezing or heating.the surviving populations of campylobacter jejuni serotypes following freezing or heat were found to be more sensitive to rifampicin and sodium deoxycholate on subsequent culture. thus while control cultures had an ic50 of greater than 20 micrograms/ml rifampicin those of injured cells were less than 5 micrograms/ml. treatment with edta caused almost identical changes in resistance suggesting that the altered resistance pattern of injured cells was due to loss of the barrier properties of the ba ...19853928571
antibacterial activity in vitro and regression studies for ceftazidime and ceftriaxone.the antibacterial activity in vitro of ceftazidime and ceftriaxone was investigated against 575 recent clinical isolates. both cephalosporins displayed excellent activity against most of the pathogens tested, in particular gram-negative bacteria, including pseudomonas aeruginosa. apart from the pseudomonas group, acinetobacter calcoaceticus and campylobacter jejuni ceftriaxone was slightly to moderately more active than ceftazidime overall. ceftriaxone was moderately active against streptococcus ...19853929558
[enteric infection due to campylobacter jejuni]. 19853930026
[survival of campylobacter jejuni in drinking water, river water and sewage].campylobacter jejuni (c. jejuni) has not been found by us in raw sewage or anaerobically stabilized sludge. therefore the survival of c. jejuni has been tested in drinking water, river water and sewage. a suspension of about 10(7) c. jejuni per ml was filled into dialysis tubes which were placed in wire baskets and exposed. exposition to drinking water was done using two continuously perfused containers in the laboratory, whereas exposition to sewage and river-water was performed at the local se ...19853939052
morphologic observations of experimental campylobacter jejuni infection in the hamster intestinal tract.the authors have developed a model for the diarrhea and intestinal lesions seen in campylobacter jejuni enterocolitis by colonizing the hamster ileum and cecum with c jejuni. erythematous inflammation of the ileum and cecum and distention of the cecum with fluid were observed at autopsy. the cecal mucosa appeared edematous. epithelial abnormalities observed by light microscopy included focal edema, occasional hyperplasia, diffuse hyperemia, and infiltration of the lamina propria with leukocytes. ...19863942198
Displaying items 901 - 1000 of 12694