| immunologic reactivity against borrelia burgdorferi in patients with motor neuron disease. | of 19 unselected patients with the diagnosis of amyotrophic lateral sclerosis (als) living in suffolk county, new york (an area of high lyme disease prevalence), 9 had serologic evidence of exposure to borrelia burgdorferi; 4 of 38 matched controls were seropositive. eight of 9 seropositive patients were male (8 of 12 male patients vs 2 of 24 controls). rates of seropositivity were lower among patients with als from nonendemic areas. all patients had typical als; none had typical lyme disease. c ... | 1990 | 2334308 |
| monoclonal antibodies specific for the outer surface protein a (ospa) of borrelia burgdorferi prevent lyme borreliosis in severe combined immunodeficiency (scid) mice. | we have recently shown that viable borrelia burgdorferi organisms induce a chronic infection associated with arthritis and carditis in severe combined immunodeficiency (scid) mice but not in immunocompetent mice. the disease is similar to that found in patients suffering from lyme disease. we now show that b. burgdorferi-specific immune mouse sera as well as a monoclonal antibody to the spirochetal outer surface antigen a (31 kda) but not monoclonal antibodies specific for the 41-kda antigenic c ... | 1990 | 2339119 |
| [value and status of neurologic, serologic, internal medicine and orthopedic studies in routine diagnosis of sudden deafness]. | one hundred and eighteen patients with acute unilateral sensorineural hearing loss were examined. diagnosis included a neurological, orthopedic, and internal medical examination. serological tests were performed including influenza-, parainfluenza-, adenovirus-, rs-virus, enterovirus-, morbilli-, varicella- and cytomegalie-virus-kbr, also the serological tests for toxoplasmosis, rheuma, lues, mycosis, and borrelia. the examinations allowed conclusions to be drawn about a possible etiology in fiv ... | 1990 | 2340040 |
| ["insect bite" and borrelia]. | a case of insect bite with a peculiar evolution is presented. the simultaneous inoculation of borrelia burgdorferi and the treatment are discussed. | 1990 | 2340942 |
| the borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): molecular cloning, expression, and amplification of the gene. | monoclonal antibodies directed against the major borrelia burgdorferi flagellar protein, the 41-kilodalton (kda) protein flagellin, were used to monitor cloning and expression of the flagellin gene from a borrelia burgdorferi genomic library. the structure of the gene was analyzed, and recombinant nonfusion flagellin was produced in escherichia coli. a dna sequence analysis of the 41-kda flagellin gene revealed the presence of an open reading frame that encoded a protein having 336 amino acid re ... | 1990 | 2341173 |
| expression of antigens from chromosomal and linear plasmid dna of borrelia coriaceae. | three recombinant plasmids containing dna from borrelia coriaceae, the putative agent of epizootic bovine abortion, expressed antigens in escherichia coli that reacted with antibodies specific for b. coriaceae. two of the recombinants each expressed a single high-molecular-weight antigen. the third recombinant expressed three smaller antigens. the dna inserts were sized and mapped. hybridization of the cloned inserts to pulsed-field electrophoresis samples of b. coriaceae whole-cell dna revealed ... | 1990 | 2341175 |
| lyme disease in the dog. | | 1990 | 2343513 |
| borrelia burgdorferi in the central nervous system: experimental and clinical evidence for early invasion. | intravenous injection into adult lewis rats of live borrelia burgdorferi, the spirochetal agent of lyme disease, was followed by increased permeability of the blood-brain barrier. permeability was measured by the ratio of 125i-labeled albumin in cerebrospinal fluid to that in blood. permeability changes were dose-dependent, began 12 h after inoculation, and reversed within 1 week. only live, intravenously inoculated organisms produced impairment of the blood-brain barrier. a spirochetal strain-d ... | 1990 | 2345299 |
| oligoclonal borrelia burgdorferi-specific igg antibodies in cerebrospinal fluid in lyme neuroborreliosis. | cerebrospinal fluid (csf) and serum from 45 patients with lymphocytic meningoradiculitis were examined by isoelectric focusing combined with immunoblotting to detect borrelia burgdorferi-specific oligoclonal immunoglobulin g (igg) bands. in pretreatment samples, 35 patients (78%) showed b. burgdorferi-specific oligoclonal igg in csf indicative of intrathecal antibody production. at 2, 3-6, and 6 weeks after onset, respectively, such bands were present in 5 (42%) of 12, 21 (88%) of 24, and in all ... | 1990 | 2345300 |
| evaluation of the intrathecal antibody response to borrelia burgdorferi as a diagnostic test for lyme neuroborreliosis. | the intrathecal antibody response to borrelia burgdorferi was evaluated in american and west german patients with lyme neuroborreliosis. by an antibody capture enzyme immunoassay, 12 (92%) of 13 patients from the usa with lyme meningitis were found to have intrathecal antibody production to b. burgdorferi, usually of multiple isotypes, most commonly iga. of 12 patients with putative late central nervous system manifestations of lyme disease, 5 (42%) had local production of igg or iga spirochetal ... | 1990 | 2345301 |
| false-positive igm antibodies to borrelia burgdorferi in indirect elisa as a result of igm rheumatoid factor. | | 1990 | 2345309 |
| summary of the first 100 patients seen at a lyme disease referral center. | purpose and patients and methods: lyme disease is a major clinical problem in a number of endemic areas in the united states. in areas where anxiety about the disease is high, patients and physicians often ascribe clinical concerns to lyme disease. incorrect diagnosis often leads to unnecessary antibiotic treatment (often prolonged or repeated intravenous therapy). this report summarizes the cases of the first 100 patients referred to the lyme disease center at robert wood johnson medical school ... | 1990 | 2346158 |
| persistent atrioventricular block in lyme borreliosis. | cardiac manifestations are reported in 0.3%-4.0% of european patients with borrelia burgdorferi (b.b.) infection. usually symptoms disappear within 6 weeks. we report a case with persistent impairment of atrioventricular (av) conduction. diagnosis was confirmed by demonstration of igm antibodies and increase of igg antibody titers against b.b. in serum, by isolation of the spirochete from skin biopsy material and by the typical clinical combination of erythema migrans, bannwarth syndrome (mening ... | 1990 | 2348647 |
| analysis of supercoiled circular plasmids in infectious and non-infectious borrelia burgdorferi. | linear plasmids are widely distributed in isolates of borrelia burgdorferi, but the prevalence of supercoiled circular (sc) plasmids has not been clearly established. enriching for sc plasmids on ethidium bromide density-gradients revealed that a low passage of strain sh-2-82 of b. burgdorferi has at least six sc plasmids (8.4, 8.8, 20, 26, 29 and 51 kb). in addition, several of the sc plasmids were observed by electron microscopy to form concatenated structures. because of the high incidence of ... | 1990 | 2348778 |
| intrasynovial production of oligoclonal specific antibodies in lyme arthritis. | | 1990 | 2348847 |
| [fibromyalgia is a syndrome not a diagnosis]. | | 1990 | 2349064 |
| survival of borrelia burgdorferi in human blood stored under blood banking conditions. | hematogenous dissemination of organisms occurs in many spirochetal diseases, including lyme disease and syphilis. although syphilis has been transmitted by transfusion, in the vast majority of cases, only fresh blood products were involved, in part because treponema pallidum survives poorly when refrigerated in citrated blood. because of the rising incidence of lyme disease in certain areas, whether its causative agent, borrelia burgdorferi, could survive under blood banking conditions was studi ... | 1990 | 2349627 |
| [granulomatous thrombophlebitis in lyme borreliosis--case report]. | the presented case report shows a patient with lyme disease who also developed thrombophlebitis saltans. histopathologically we found a granulomatous perivasculitis, an immunohistological investigation showed deposits of igg, iga, igm, c3 and c4 in the vessels. antibody titers against borrelia burgdorferi were elevated, but no anti-cardiolipin antibodies were found. a pathogenetic correlation due to anti-phospholipid antibodies that have been found in thrombosis and borreliosis or a vasculitis v ... | 1990 | 2351413 |
| serologic analyses of cottontail rabbits for antibodies to borrelia burgdorferi. | an enzyme-linked immunosorbent assay was developed to detect antibodies to borrelia burgdorferi in cottontail rabbits captured in millbrook, n.y., and new york, n.y. five antigenically variable strains of b. burgdorferi were analyzed to determine the variability of serologic test results. in analyses of 79 serum samples, seropositivity ranged from 56% for a strain cultured from kidney tissues of a cottontail rabbit to 68% for a strain isolated from a larva of ixodes dentatus, a tick that parasit ... | 1990 | 2351732 |
| [elisa for specifying oligoclonal bands of isoelectric focusing of cerebrospinal fluid in patients with neuroborreliosis and multiple sclerosis]. | | 1990 | 2352572 |
| histopathological findings in the central and peripheral nervous systems in neuroborreliosis. a report of three cases. | very little is known about tissue changes and pathophysiological mechanisms in garin-boujadoux-bannwarth syndrome. we report histopathological findings in the central and peripheral nervous systems of three typical cases. in the acute stage of the disease mononuclear perivascular infiltrations with mainly t-helper cells were the prominent finding, whereas after treatment there was no vessel involvement. the fibre changes in the peripheral nervous system consisted of axonal degeneration. no borre ... | 1990 | 2355235 |
| anti-tick antibodies: an epidemiologic tool in lyme disease research. | in 1988, antibodies to arthropod (ixodes dammini, dermacentor variabilis, and aedes aegypti) salivary gland proteins and to borrelia burgdorferi were measured by enzyme-linked immunosorbent assay in 53 high-risk outdoor workers from the new jersey department of environmental protection. lyme disease seropositives had significantly higher anti-i. dammini antibody levels than seronegative controls (p = 0.006). anti-b. burgdorferi antibody (enzyme-linked immunosorbent assay) and anti-i. dammini ant ... | 1990 | 2356814 |
| articular involvement in european patients with lyme disease. a report of 32 italian patients. | lyme disease (ld) is a well recognized multisystem disorder, caused by the spirochaete borrelia burgdorferi. it involves the skin, nervous system and heart. arthritis is described in 50-60% of american patients but seems to be less common in europe. we studied the pattern of articular involvement in a group of 67 italian patients with ld. thirty-two (48%) of 67 had rheumatological manifestations. the prevalence of arthritis in our patients was 16/67 (24%), the remaining 16 experiencing only arth ... | 1990 | 2357498 |
| reactivity of european and american isolates of borrelia burgdorferi with different monoclonal antibodies by means of a microimmunoblot technique. | ten european and 3 north american isolates of borrelia burgdorferi were compared as to their reactivity with 9 mouse monoclonal antibodies (mma) to the type strain b. burgdorferi b31, and 1 mma directed against b. hermsii. a treponema pallidum strain was used for a genus-specific control. differences in the protein patterns of the european and the north american strains were mostly based on the absence of distinct ospa and ospb bands. the ospa mmas h 5332 and h 3ts were reactive with 3 european ... | 1990 | 2360965 |
| [encephalomyelitis with elevated serum antibody against borrelia burgdorferi]. | a 60-year-old female was admitted because of intermittent fever, arthralgia, itching of whole body, pretibial edema, urinary incontinence, pain of both legs and gait disturbance, after an insect bite. on admission, she had fever of 38 degrees c, and nuchal pain and stiffness. neurological examination revealed spasticity of lower legs and increased deep tendon reflexes of all extremities. hyperesthesia and hyperalgesia were noted on c2-4 and l5-s5 areas. leukocyte count was 10,100/mm3 and crp was ... | 1990 | 2364635 |
| characterization of borrelia burgdorferi proteins reactive with antibodies in synovial fluid of a patient with lyme arthritis. | four borrelia burgdorferi proteins reactive with antibodies in the synovial fluid of a patient with lyme arthritis were characterized. homology between amino acid sequences of immunoreactive spirochetal proteins and human proteins, including members of the escherichia coli groel protein family, suggests that antigenic mimicry may play a role in the pathogenesis of lyme arthritis. | 1990 | 2365463 |
| [the infectiousness of ixodes persulcatus ticks with the causative agents of lyme disease and tick-borne encephalitis simultaneously]. | by means of individual bacteriological and virological investigations of adult ixodes persulcatus ticks and comparison of obtained results there has been established their simultaneous spontaneous infection with agents of lyme disease and tick-borne encephalitis. a possible frequency of such mixed infection in these vectors is being discussed. | 1990 | 2367143 |
| borrelia burgdorferi infection: should one treat before confirming clinical diagnosis? | | 1990 | 2370207 |
| prevalence of tick-borne encephalitis and lyme borreliosis in a defined swedish population. | sera from 346 individuals living on lisö, an area south of stockholm, endemic for tick-borne encephalitis (tbe) and lyme borreliosis, were tested for antibody activity to tbe-virus (tbev) and borrelia burgdorferi, using a sonicate antigen, by haemagglutination-inhibition and elisa, respectively. 10/346 (3%) individuals had a history of previous serologically confirmed tbe with encephalitic symptoms, and 33/346 (10%) had a history of previous erythema chronicum migrans (ecm). four individuals (1% ... | 1990 | 2371545 |
| aspects of the diagnosis of lyme borreliosis. | attempts were made to culture spirochetes from the cerebrospinal fluid of 105 patients with suspected lyme borreliosis with neurological complications. at the final evaluation, only 38 patients fulfilled the criteria of neuroborreliosis. spirochetes were cultured from the cerebrospinal fluid of four of these patients. all four had pleocytosis in their cerebrospinal fluid and a history of neurological symptoms of only four to ten days. two had no detectable antibodies in their cerebrospinal fluid ... | 1990 | 2371553 |
| borrelia burgdorferi: survival in experimentally infected human blood processed for transfusion. | the isolation of borrelia burgdorferi from blood raises the possibility of bloodborne transmission of lyme borreliosis through transfusions. to assess this possibility, the ability of b. burgdorferi to survive in human blood processed for transfusion was studied. human blood was inoculated with b. burgdorferi type strain b-31 (atcc 35210) at 0.2, 20, or 2000 viable cells/ml, processed by conventional blood banking procedures, stored at 4 degrees c, and cultured for b. burgdorferi at 12, 23, 36, ... | 1990 | 2373880 |
| [occurrence of borrelia burgdorferi in ixodes ricinus in the netherlands]. | in a 1989 surveillance to study the infection rate of ixodes ricinus ticks with borrelia burgdorferi, a total of 1838 nymphal and adult ixodes ricinus ticks were collected from 20 locations in the netherlands. by means of a culture method using modified barbour-kelly-stoenner medium nymphs were examined in pools of 4 and adults were examined individually. with 63 out of 75 isolates propagation and serological identification were possible. all of these 63 isolates were identified as borrelia burg ... | 1990 | 2374615 |
| [the occurrence of antibodies to borrelia in dogs]. | using the method of indirect hemagglutination and an antigenic extract of borrelia recurrentis the occurrence of antibodies to borrelias was investigated in 169 sera of dogs from two groups with different exposure to vectors. a significant difference in the serum positivity of dogs coming to prague outpatient veterinary wards (53.7%) and of dogs coming from laboratory packs (20.9%) confirms the participation of dogs in the epidemiology of lyme borreliosis, indicates the influence of the environm ... | 1990 | 2375071 |
| neuroborreliosis simulating a progressive stroke. | a 55-year-old man with an onset and course of neurological symptoms of a progressive stroke was found to have a cns infection caused by the borrelia spirochete. almost complete recovery was seen after intravenous infusion of bencylpenicillin. elevated borrelia igg antibody titers could be seen for long time after recovery. the possibility of an infection due to neuroborreliosis must be considered. if ct scan doesn't show any focal ischemic or hemorrhagic area further investigation with lumbar pu ... | 1990 | 2375251 |
| lyme disease. employee education and treatment. | lyme disease is a complex multisystem disorder that begins with the bite from the ixodes tick. the ixodes tick carries the spirochete, borrelia burgdorferi, and infects humans with the spirochete. the initial symptoms of lyme disease are the erythema chronicum migrans, or "bull's eye" rash, and flu-like symptoms. stage iii of lyme disease can occur weeks to months after the initial infection when more serious complications may develop, such as neurologic, cardiac, or ophthalmologic problems. if ... | 1990 | 2375798 |
| [infestation of ticks with borrelia burgdorferi and francisella tularensis in slovakia]. | examination of ticks for the presence of f. tularensis resulted in the isolation of 29 strains, predominantly from the species d. reticulatus (19x), further from i. ricinus (8x) and from h. concinna (2x). the results confirmed the activity of the known endemic focus in the area of podunajské biskupice and revealed three new foci in the localities smolenice (district of trnava), olichov (district of nitra), and plást'ovce (district of levice). in the focus of podunajské biskupice the mean infesta ... | 1990 | 2376012 |
| [choriocapillaritis (so-called pigment epitheliitis) in borrelia burgdorferi seroconversion]. | a significant b. burgdorferi seroconversion with an igm of 1:80 was observed in a patient complaining of loss of vision in his right eye. the finding correlated morphologically with hyperpigmentation and depigmentation in the macular region, similar to pigment epitheliitis or choriocapillaritis. in the course of the following four weeks the igm decreased by four titer steps. the visual acuity of the patient's right eye recovered from 0.4 on admission to 1.0 on discharge. the angiogram did not re ... | 1990 | 2376946 |
| simultaneous infection with an ehrlichia and borrelia burgdorferi in a child. | | 1990 | 2378481 |
| cross-reactivity of nonspecific treponemal antibody in serologic tests for lyme disease. | serum samples obtained from 59 persons who had acute necrotizing ulcerative gingivitis, periodontitis, syphilis, or lyme disease were tested against treponema phagedenis biotype reiter, treponema denticola, treponema vincentii, and treponema scoliodontum by indirect fluorescent-antibody staining methods. although there were positive reactions for sera representing each of these study groups and for 20 (13%) of 156 samples collected from the general population (premarital screening for syphilis), ... | 1990 | 2380356 |
| reactivity of human lyme borreliosis sera with a 39-kilodalton antigen specific to borrelia burgdorferi. | borrelia burgdorferi is the causative agent of lyme borreliosis, a spirochetal illness with a variety of acute clinical manifestations that may lead to debilitating neurological and arthritic complications. diagnosis is difficult because symptoms mimic a variety of unrelated clinical conditions, spirochetes cannot always be isolated from infected patients, and current serological tests are frequently inconclusive because of the presence of cross-reacting non-b. burgdorferi antibodies. to identif ... | 1990 | 2380361 |
| the 83-kilodalton antigen of borrelia burgdorferi which stimulates immunoglobulin m (igm) and igg responses in infected hosts is expressed by a chromosomal gene. | we report the cloning and characterization of a chromosomal gene from borrelia burgdorferi expressing an 83-kilodalton protein antigen in escherichia coli. the antigen reacted strongly with antisera from two human lyme disease patients. the chromosomal gene was expressed in a 6.5-kilobase-pair clai fragment cloned into a variable-reading-frame plasmid vector. | 1990 | 2380391 |
| ceftriaxone therapy of chronic inflammatory arthritis. a double-blind placebo controlled trial. | to determine whether chronic inflammatory arthritis may respond to antibiotic therapy (implying a bacterial origin), we conducted a placebo-controlled, double-blind study. sixty patients with inflammatory arthritis and antibody titers to borrelia burgdorferi 1:64 or more were randomized to receive placebo (n = 20) or 2 g/d of ceftriaxone intravenously (n = 40) for 2 weeks. two of 20 placebo- and 19 of 40 antibiotic-treated patients improved. at 1 month, the placebo-treated patients could elect t ... | 1990 | 2383162 |
| juxtaposition of expressed variable antigen genes with a conserved telomere in the bacterium borrelia hermsii. | borrelia hermsii, an agent of relapsing fever, survives in mammals through antigenic variation. change in serotype-specific variable outer membrane proteins (vmps) occurs when a vmp gene at an expression site is replaced with a previously silent gene for another vmp. silent and active genes are on separate linear plasmids. the upstream site for a nonreciprocal recombination between two linear plasmids is near the 5' ends of the expressed and silent genes. in the present study we sought the downs ... | 1990 | 2385585 |
| status of ixodes dammini (acari: ixodidae) in illinois. | ixodes dammini spielman, clifford, piesman & corwin was found for the first time in illinois in november 1987, when two adult females were collected from two deer in jo daviess county in the northwestern corner of the state. in 1988, in a study of six state parks in northern illinois, questing adults and nymphs were encountered in one park in ogle county. during the firearm deer hunt in november 1988, adult female and male ticks were found in several counties, with a high rate of infestation (gr ... | 1990 | 2388231 |
| intrinsic competence of three ixodid ticks (acari) as vectors of the lyme disease spirochete. | we compared the intrinsic vector competence of ixodes dammini spielman et al., dermacentor variabilis (say), and amblyomma americanum (l.) for the lyme disease spirochete (borrelia burgdorferi johnson et al.) on prudence island, rhode island, a lyme disease-endemic site where all three ticks occur together. natural and experimental spirochete infection rates were determined in those ticks and their degree of contact with white-footed mice (peromyscus leucopus), the principal reservoir host, was ... | 1990 | 2388239 |
| the role of medium-sized mammals as reservoirs of borrelia burgdorferi in southern new york. | the ability of raccoons (procyon lotor), striped skunks (mephitis mephitis) and opossums (didelphis virginiana) to serve as reservoirs of borrelia burgdorferi, the spirochetal agent of lyme disease, was compared with that of white-footed mice (peromyscus leucopus). twenty-eight (28) medium-sized mammals and 34 white-footed mice were captured in westchester county, new york (usa) in summer 1986. animals were caged over pans of water for 1 to 2 days to recover engorged tick larvae (ixodes dammini) ... | 1990 | 2388356 |
| [multiple sclerosis or lyme disease? a diagnosis problem of exclusion]. | there is no diagnostic biological marker in multiple sclerosis. thus, its diagnosis is based on clinical criteria. these criteria can also be found in other conditions. lyme disease is currently among them. in a late period of the disease demyelinating involvement of central nervous system can develop, and multiple sclerosis can be erroneously diagnosed. we have evaluated a series of 55 patients with a definite diagnosis of multiple sclerosis, and we have found evidence of infection by the causa ... | 1990 | 2388492 |
| cloning and sequence analysis of linear plasmid telomeres of the bacterium borrelia burgdorferi. | borrelia burgdorferi, the lyme disease agent, has double-stranded linear plasmids with covalently closed ends. dna at the ends, or telomeres, of two linear plasmids of b. burgdorferi strain b31 was examined. telomeric sequences from both ends of a 16 kb linear plasmid and from one end of a 49 kb linear plasmid were cloned and sequenced. an 18 bp at-rich inverted repeat was found at each end of the 16 kb linear plasmid. the sequences of the two ends of this plasmid were different beyond these sho ... | 1990 | 2388560 |
| lyme borreliosis-associated encephalopathy. | borrelia burgdorferi infection (lyme disease) is frequently accompanied by cns dysfunction. particularly common is a mild confusional state, the mechanism of which is unknown. since cns infection with b burgdorferi is usually accompanied by intrathecal synthesis of specific antibody, we studied csf in 73 patients referred for presumed cns lyme, manifested primarily as this confusional state. of 30 seropositive patients evaluated, only 5 had intrathecal antibody production. seven seronegative pat ... | 1990 | 2392213 |
| lyme disease. | | 1990 | 2392409 |
| [determination of antibodies against borrelia burgdorferi in patients with morphea, lichen sclerosus et atrophicus and erythema chronicum migrans]. | several cutaneous entities described in europe as chronic migrans erythema (cme), mild cutis lymphadenosis (mcl) and chronic atrophyc acrodermatitis (caa) constitute clinical manifestations of a borrellia burgdorferi. the presence of clinical and hystologic lesions similar to those of liquen esclerosus and atrophyc (lea) and localized esclerodermia (morphea) in patients with caa has driven to several authors to demonstrate the aethiologic participation of b. burgdorferi in patients carrying thos ... | 1990 | 2392594 |
| spirochetal forms in the dermal lesions of morphea and lichen sclerosus et atrophicus. | morphea and lichen sclerosus et atrophicus are cutaneous diseases that are manifest by an early edematous stage, followed later by sclerosis and atrophy. they share features with acrodermatitis chronica atrophicans and erythema chronicum migrans, diseases that have been linked to infection by the spirochete borrelia burgdorferi. a modified silver stain was used to identify the presence of spirochetes in skin biopsy specimens of patients with morphea and lichen sclerosus et atrophicus. spirocheta ... | 1990 | 2393064 |
| two genomic species in borrelia burgdorferi. | a total of 13 borrelia burgdorferi strains (responsible for lyme borreliosis) and representatives of 3 other borrelia species (b. hermsii, b. parkeri, b. turicatae) associated with relapsing fever were studied by dna/dna hybridization and rrna gene-restriction patterns. two genomic dna hybridization groups were observed which could be differentiated by rrna gene-restriction patterns. moreover, the number and size of restriction fragments suggest the existence of a single set of 16 and 23 s rrna ... | 1990 | 2399368 |
| detection of borrelia burgdorferi dna in museum specimens of ixodes dammini ticks. | in order to investigate the potential for borrelia burgdorferi infection before the recognition of lyme disease as a clinical entity, the polymerase chain reaction (pcr) was used to examine museum specimens of ixodes dammini (deer ticks) for the presence of spirochete-specific dna sequences. one hundred and thirty-six archival tick specimens were obtained representing various continental u.s. locations; dna sequences characteristic of modern day isolates of b. burgdorferi were detected in 13 194 ... | 1990 | 2402635 |
| comparison of indirect immunofluorescent-antibody assay, enzyme-linked immunosorbent assay, and western immunoblot for the diagnosis of lyme disease in dogs. | enzyme-linked immunosorbent assay (elisa), indirect immunofluorescent-antibody assay (ifa), and western immunoblot were used to test serum samples from 128 dogs for the presence of antibody to borrelia burgdorferi. sera included 72 samples from dogs suspected of having lyme disease, 32 samples from dogs residing in areas in which lyme disease was not considered endemic, and 24 samples from dogs with clinical and serologic evidence of immune-mediated disease (n = 10), rocky mountain spotted fever ... | 1990 | 2405018 |
| lyme disease. | lyme disease is an infectious, immune-mediated, multisystem disease. recent epidemiologic data confirms that in the united states, lyme disease is spreading faster than any other infectious disease except aids. the bacteria that causes lyme disease, borrelia burgdorferi, is transmitted to humans by the bite of the deer tick, ixodes dammini. if diagnosed and treated in its early stages, lyme disease is less likely to result in later cardiac, neurologic, and arthritic complications. arthritic comp ... | 1990 | 2407990 |
| [recurrent and relapsing course of borreliosis of the nervous system]. | the clinical variability of chronic infections due to borrelia burgdorferi is greater than previously thought. three personal cases are presented in an overview, together with cases from the literature. chronic spastic para- and quadriparesis, transverse myelitis and recurrent hemiparesis have been noted in such cases. frequently, there is additional involvement of the cranial nerves. chronic polyneuritis, mononeuritis multiplex, as well as plexus neuritis can also occur. psychiatric manifestati ... | 1990 | 2408240 |
| variable major proteins of borrelia hermsii. epitope mapping and partial sequence analysis of cnbr peptides. | the variable major proteins (vmp) of serotypes 7 and 21 of the relapsing fever agent borrelia hermsii were isolated by detergent extraction and high performance liquid chromatography. cyanogen bromide (cnbr) digestion of the isolated vmp yielded two peptides of apparent molecular weights 20,000 (20 k) and 16 k from vmp7, and three peptides of 14.5, 14, and 7 k mol wt from vmp21. serotype-specific monoclonal antibodies bound in western blots to one of each of the two or three cnbr fragments from ... | 1985 | 2409197 |
| heterogeneity of major proteins in lyme disease borreliae: a molecular analysis of north american and european isolates. | we examined 46 isolates of borrelia burgdorferi, the etiologic agent of lyme disease and related disorders, with polyacrylamide gel electrophoresis and monoclonal antibodies. our attention was on the ospa proteins, which are major proteins of the spirochete. there were at least four discernible phenotypes of the ospa protein. while 25 north american isolates were, with one exception, homogeneous in the type of ospa protein that they produced, 21 european isolates were heterogeneous in the types ... | 1985 | 2411827 |
| tick-borne relapsing fever in a premature infant. | relapsing fever is caused by the borrelia species of spirochetes. louse-born epidemics of the disease may occur but the endemic disease is usually transmitted to humans by the bite of an infected tick (ornithodorus). transplacental infection was suggested more than 75 years ago (1) but has been rarely documented (2). we describe a case of neonatal relapsing fever where maternal infection was the probable cause of the premature delivery and infection in the infant. | 1985 | 2415056 |
| expression of hla-dq antigens on keratinocytes in borrelia spirochete-induced skin lesions. | skin biopsies were investigated with two different immunohistochemical techniques, thus revealing hla-dq antigens on hla-dr-expressing keratinocytes in the late skin manifestations of a borrelia spirochete infection. in the early skin lesions only hla-dr antigens were present on the keratinocytes. the invariant gamma chain of class ii transplantation antigens was observed on keratinocytes in 1:5 of the late cases. upon penicillin treatment detectable hla-dr and hla-dq antigens disappeared comple ... | 1986 | 2419972 |
| serologic studies of erythema chronicum migrans afzelius and acrodermatitis chronica atrophicans with indirect immunofluorescence and enzyme-linked immunosorbent assays. | to determine whether antibodies to borrelia spirochetes were present, sera from 88 patients with uncomplicated erythema chronicum migrans afzelius (ecma), from 9 patients with ecma-related extracutaneous complications and from 26 patients with acrodermatitis chronica atrophicans (aca) were submitted to an enzyme-linked immunosorbent assay (elisa) and an indirect immunofluorescence (if) assay. the assays were calculated to be 95% specific. there was good correlation between the if test with a pol ... | 1985 | 2420115 |
| lyme disease during pregnancy. | lyme disease is an increasingly recognized tick-borne illness caused by a spirochete, borrelia burgdorferi. because the etiologic agent of lyme disease is a spirochete, there has been concern about the effect of maternal lyme disease on pregnancy outcome. we reviewed cases of lyme disease in pregnant women who were identified before knowledge of the pregnancy outcomes. nineteen cases were identified with onset between 1976 and 1984. eight of the women were affected during the first trimester, se ... | 1986 | 2423719 |
| erythema chronicum migrans of lyme disease: diagnosis by monoclonal antibodies. | we demonstrated spirochetes from a skin lesion of the erythema chronicum migrans in a patient with lyme disease by the murine monoclonal antibody (h9724). frozen skin biopsy specimens reacted with murine monoclonal antibodies (h5332, h9724, and h4825) and were examined by indirect immunofluorescence assay. the spirochetes stained with monoclonal antibody h9724 and fluorescein isothiocyanate-conjugated second antibody (goat antimouse igg). the antibody h9724 is specific for the periplasmic flagel ... | 1986 | 2426315 |
| comparative prevalence of babesia microti and borrelia burgdorferi in four populations of ixodes dammini in eastern massachusetts. | we determined the prevalence of babesia microti and borrelia burgdorferi in four populations of ixodes dammini in eastern massachusetts. the feulgen's reaction was more sensitive than the giemsa method for detecting salivarian babesia. a combination of darkfield and direct-fluorescent-antibody examination proved more sensitive than either method alone for detecting spirochetal infection. the prevalence of spirochetes was greater than the prevalence of babesia in each of the tick populations stud ... | 1986 | 2430433 |
| the spirochetal etiology of lymphadenosis benigna cutis solitaria. | ten patients with a clinical diagnosis of lymphadenosis benigna cutis (labc) solitaria were investigated. four of the patients knew that they had received a tick bite during the year prior to the appearance of the labc lesion. five patients had had a migrating erythema, indicating a previous erythema chronicum migrans afzelius, near the site where the labc lesion had developed. in one patient meningitis and a facial palsy occurred. elevated titers of antibodies against borrelia spirochetes were ... | 1986 | 2433866 |
| acrodermatitis chronica atrophicans in association with lichen sclerosus et atrophicans: tubulo-interstitial nephritis and urinary excretion of spirochete-like organisms. | we report about a 38-year-old male patient with coexisting acrodermatitis chronica atrophicans, lichen sclerosus et atrophicans and recurrent diabetic metabolic disorders since 9 years. serologically igg antibodies against borrelia burgdorferi could be detected. moveless winded structures, morphologically resembling borreliae could be demonstrated in the urine sediment by dark field microscopy. additionally a tubulo-interstitial nephritis was diagnosed by the presence of a dysmorphic hematuria, ... | 1987 | 2436416 |
| isolation of the outer envelope from borrelia burgdorferi. | borrelia burgdorferi consists of an inner protoplasmic cylinder, containing the genome and cytoplasmic elements, surrounded by a number of axial filaments, all completely encased within a multiple-layered outer envelope structure (oe). in this study, a sodium dodecyl sulfate-mediated technique was used to isolate the oe from borrelia burgdorferi in an attempt to better understand this structure in terms of its antigenic reactivity to lyme disease patient sera. electron microscopic evidence sugge ... | 1986 | 2437735 |
| an ige response to spirochete antigen in patients with lyme disease. | most but not all lyme disease patients produce specific ige antibodies to borrelia burgdorferi. development of ige antibodies paralleled that of other immunologic classes and appeared to be directed against a polypeptide with a molecular weight of 41,000. total serum ige levels in lyme disease patients were usually within the normal range in all stages of the disease. however, highly elevated total serum ige in certain patients were not correlated to any particular disease stage nor to specific ... | 1986 | 2437736 |
| diagnosis of lyme disease by demonstration of spirochetes in tissue biopsies. | in 14 patients with lyme disease skin biopsies, synovial specimens and a lymphnode showed lymphohistiocytic infiltrates, often perivascular. obliterative alterations of blood vessels and fibrinoid were characteristic for infection of synovium. spirochetes were demonstrated in all specimens by the bosma-steiner silver impregnation method. spirochetes were localized in dermal papilles and subepidermal zones of skin sections of ecma, in lymph vessels and collagen fibres. in synovia of patients with ... | 1986 | 2437737 |
| polymorphisms of major surface proteins of borrelia burgdorferi. | forty-five isolates of borrelia burgdorferi, the etiologic agent of lyme disease, were examined with monoclonal antibodies directed against the surface proteins, ospa and ospb. eighteen of 21 strains of european origin could be distinguished from 24 north american strains on the basis of their reactivities with two antibodies, one specific for an ospa protein and one specific for an ospb protein. differences in antibody reactivities were associated with differences in the electrophoretic migrati ... | 1986 | 2437738 |
| immunochemical and immunological analysis of european borrelia burgdorferi strains. | borrelia burgdorferi strains (n = 23), isolated from patients (n = 8) and ticks (n = 14), were analyzed by sds page and western blotting with monoclonal antibodies and polyclonal sera (rabbit immune serum and sera from patients). testing the 23 strains by sds page 9 different patterns of major protein bands were observed. in contrast to us strains some of our strains showed only weak or negative reactivity with the ospa specific monoclonal antibody h5332. analysis with polyclonal sera gave furth ... | 1986 | 2437739 |
| demonstration of spirochaetes in patients with lyme disease with a modified silver stain. | spirochaetes were demonstrated in material from patients with lyme disease by short-time high-concentrate silver impregnation after treatment with amylase. removal of mucoid material was essential to visualise borrelia burgdorferi. lyme spirochaetes were demonstrated in material from 23 patients with lyme disease--erythema chronicum migrans (ecm) 10, lymphadenosis benigna cutis (labc) 7, arthritis 4 and bannwarth's syndrome 2. spirochaetes were localised in the subepidermal zone, peri- and intra ... | 1987 | 2438410 |
| evidence for spirochetal origin of circumscribed scleroderma (morphea). | acrodermatitis chronica atrophicans (aca) and morphea are clinically distinct skin diseases with some common features and possible coexistence. we found antibodies to borrelia burgdorferi in eight of fifteen patients with morphea. six of them had igg antibodies and two both igg and igm antibodies. four of the eight seropositive and five of the seven seronegative patients had been treated with high dose penicillin previously. spirochetal organisms could be cultured in barbour-stoenner-kelly's med ... | 1987 | 2442935 |
| a murine igm monoclonal antibody binds an antigenic determinant in outer surface protein a, an immunodominant basic protein of the lyme disease spirochete. | a hybridoma cell line formed by the fusion of the p3x63-ag8.653 myeloma cell line with splenocytes from balb/c mice immunized with borrelia burgdorferi produced an igm monoclonal antibody (mab-11g1) with kappa-light chains which detected an antigenic determinant in a major spirochetal protein of m.w. approximately 31,000, also known as outer surface protein a (osp-a). apparent saturation was reached in approximately 35 min with 34 ng of mab-11g1 binding to 5 x 10(7) spirochetes giving an estimat ... | 1988 | 2447170 |
| [primary lymphoma of the nervous system following radiculoneuritis caused by borrelia burgdorferi: study of a case]. | a 50-year-old man had a primary lymphoma of the central nervous system one year after a tick bite and a radiculoneuritis secondary to borrelia burgdorferi infection. determination of antibodies against borrelia burgdorferi revealed increasing igm and igg titers, and the autopsy showed a primary b-cell immunoblastic lymphoma of the brain without evidence of extraneural lymphoproliferative disorder. lymphoma of the brain is a rare type of central nervous system cancer, and sporadic cases appear wi ... | 1988 | 2451279 |
| biological activity of borrelia burgdorferi antigens. | osp-a (approximately 31 kda) and flagellins (approximately 41 kda) are prominent antigens of borrelia burgdorferi. both osp-a and flagellins are immunogenic in patients and in experimentally infected mice and hamsters, but the kinetics of antibody formation to each vary considerably between the species. the role of eluted osp-a and flagellins in the cellular immune response, chemotaxigenesis, and cytoadherence was measured. eluted osp-a and flagellins stimulated the proliferation of normal and i ... | 1988 | 2461134 |
| antigenic variability of borrelia burgdorferi. | borrelia burgdorferi strains (six isolates from north america and 28 isolates from europe) were analyzed by physicochemical and immunological methods. by sds-page, all borrelia burgdorferi strains tested had two major proteins with constant molecular weights of 60 and 41 kda and one, two, or three variable low molecular weight proteins (ospa = 30-32 kda, ospb = 34-36 kda, pc = 21-22 kda). all combinations--except ospb alone or ospb/pc--were observed. borrelia burgdorferi strains were different f ... | 1988 | 2461135 |
| demonstration of antigen-specific t cells and histopathological alterations in mice experimentally inoculated with borrelia burgdorferi. | antigen-specific t-cell responses and histopathological changes were studied in mice experimentally inoculated with borrelia burgdorferi b31. inbred mice with different h-2 haplotypes and/or different genetic backgrounds were inoculated with b. burgdorferi organisms and tested for antigen-specific t-cell responses in vivo (delayed-type hypersensitivity [dth]) and in vitro (t-cell proliferation). comparable dth responses were found after inoculation with either inactivated (in the presence of adj ... | 1989 | 2462540 |
| isolation and characterization of borrelia burgdorferi-specific and autoreactive t-cell lines from the cerebrospinal fluid of patients with lyme meningoradiculomyelitis. | | 1988 | 2462820 |
| lyme disease. | lyme disease is of interest to the neurologist and neuroscientist for a variety of reasons. as more arthropod hosts throughout the world are infected with the causative organism, borrelia burgdorferi, the illness in humans is becoming more prevalent; in addition, recognition of the disease in humans and susceptible animals is increasing. the neurological manifestations include acute and chronic forms, and it has become clear that b. burgdorferi has joined treponema pallidum, herpes simplex virus ... | 1989 | 2472690 |
| shared flagellar epitopes of borrelia burgdorferi and borrelia anserina. | antigenic cross-reactivity between borrelia burgdorferi and borrelia anserina was studied using mouse immune sera and monoclonal antibodies. with immune sera, significant cross-reactivity between b. burgdorferi and b. anserina was demonstrated by indirect immunofluorescent assay. in immunoblots, most of the cross-reactivity was shown to be associated with the periplasmic flagella. using monoclonal antibodies in immunoblots, it was shown that b. burgdorferi and b. anserina shared at least two fla ... | 1989 | 2473569 |
| immune responses to borrelia burgdorferi in patients with reactive arthritis. | in reactive arthritis (rea), including reiter's syndrome, a close relationship between chronic enteric and genitourinary infections and the clinical features of enthesitis has been described. in contrast, in lyme arthritis, a distinct clinical entity, chronic infection with the tick-transmitted spirochete borrelia burgdorferi has been associated with the disease. in a prospective study, 51 patients with rea were tested for evidence of chlamydial and spirochetal infection. the presence of chlamyd ... | 1989 | 2476133 |
| [effect of borrelia--the causative agent of tick-borne relapsing fever--on the vector]. | direct or indirect evidence of pathogenic effect of borrelia sogdiana, a tick-borne recurrent fever causative agent, on ornithodoros papillipes is obtained, pointing to a parasitic character of their relations. differences between infected and uninfected ticks are revealed both under normal life conditions (lower survival, more intensive process of moult), as well as under the effect of poison. higher sensitivity of infected ticks to ddt was established experimentally; hcch effect was determined ... | 1989 | 2476652 |
| temporary pacing in complete heart block due to lyme disease: a case report. | the authors present the case of a 44-year-old man who was admitted with complete heart block and signs of severe bradycardia. after steroid administration and temporary pacemaker treatment the complete heart block resolved. during this therapy transient st segment and t wave abnormalities occurred. the positive borrelia burgdorferi antibody titer arrived only after therapy had been completed. this is regarded as the first case of lyme carditis with complete heart block diagnosed in eastern europ ... | 1989 | 2476769 |
| evidence for spirochetal origin of sudeck's atrophy (algodystrophy, reflex sympathetic dystrophy). | four female patients with sudeck's atrophy (sa) were evaluated at our department. sera of all patients were investigated for antibodies against borrelia burgdorferi. in all patients elevated igg antibody titers and in one patient also elevated igm titer were found. in one patient structures identical with b. burgdorferi could be detected on histological sections from the skin of the affected limb. our data and those of other authors suggest that a spirochetal infection can be regarded as an impo ... | 1989 | 2476974 |
| bilateral keratitis in lyme disease. | lyme disease, caused by the spirochete borrelia burgdorferi, has ophthalmic manifestations. the authors describe two cases of lyme keratitis characterized by multiple focal, nebular opacities at varying levels of the stroma which may progress to edema, neovascularization, and scarring. close observation, in addition to systemic antibiotic therapy, may be sufficient if the visual axis is not involved, and the patient is asymptomatic. | 1989 | 2477779 |
| molecular mimicry and lyme borreliosis: a shared antigenic determinant between borrelia burgdorferi and human tissue. | the pathogenesis of chronic manifestations in lyme borreliosis, a disease induced by borrelia burgdorferi, is at present unresolved. by testing monoclonal antibodies directed against various borrelia antigens, we found an antigenic determinant shared by the 41 kda flagella protein and human tissue, especially prominent on myelinated fibers of human peripheral nerve, on nerve cells and axons of the central nervous system, as well as on certain epithelial cells (including joint synovia) and on hea ... | 1989 | 2481425 |
| structural features of borrelia burgdorferi--the lyme disease spirochete: silver staining for nucleic acids. | borrelia burgdorferi--the lyme disease spirochete--was grown in modified kelly medium and characterized by transmission and by scanning electron microscopy. using silver staining procedures which preferentially bind to nuclear components of eukaryotic cells, signal could be detected by backscattered electron imaging throughout the length of the prokaryotic spirochete. interestingly, however, the highest levels of backscattered signal were observed in naturally elaborated membrane blebs that were ... | 1989 | 2482525 |
| [lymphocytic meningoradiculitis (bannwarth's syndrome). neurologic involvement of lyme disease]. | we describe a patient with lyme's disease who showed neurologic symptoms of meningoradiculitis (bannwarth's syndrome) and had no previous history of exposure to tick bite or chronic erythema migrans. he had longterm fever and bilateral facial paralysis. antibody titres for borrelia burgdorferi in serum and cerebrospinal fluid (csf) were increased. the csf changes showed an intrathecal production of immunoglobulins (presence of plasmatic cells and immunoblasts; igg-csf: igg-serum ratio much highe ... | 1989 | 2490177 |
| [prevalence of antibodies against borrelia burgdorferi among the population of seville]. | in order to determine the prevalence of antibodies against borrelia burgdorferi in the area of sevilla, we have studied 300 healthy subjects of different age and sex groups by means of iif. of the 300 studied sera, only 4% had antibodies at titers greater than or equal to 1:16, a fact that shows the low prevalence of antibodies against this microorganism in the area. such titers were mainly found among subjects over 40 years (p less than 0.001). | 1989 | 2490479 |
| [new advances in rheumatology]. | (1) molecular pharmacology which is a starting to reach the market place, what is expected to be the next big step in the biotechnology revolution: the creation of complex compounds that are custom-designed to meet human needs. the new technology known as protein engineering promises to take us one step further by modifying natural proteins or by creating new ones especially by using somatic gene transfer and germ gene transfer and its influence on progeny. by doing this we hope to control some ... | 1989 | 2491405 |
| autoimmunity in lyme disease: molecular cloning of antigens recognized by antibodies in the cerebrospinal fluid. | in inflammatory disease of the central nervous system (cns), oligoclonal bands of immunoglobulin with restricted heterogeneity can often be observed in cerebrospinal fluid (csf) samples. these antibodies can be directed against the disease inducing pathogen or might be autoreactive and involved in the process of brain inflammation and demyelination. we used a molecular biology approach to characterize these antibody responses in patients with lyme disease. this disorder is caused by infections w ... | 1989 | 2491615 |
| another swedish family with complete properdin deficiency: association with fulminant meningococcal disease in one male family member. | inherited deficiency of the complement component properdin is described in a swedish family without any previous history of meningococcal infections. the properdin-deficient index patient died from a fulminant infection caused by neisseria meningitidis serogroup y. family investigation revealed properdin deficiency in the patient's half-brother and in the maternal grandfather. the half-brother had a history of pneumococcal pneumonia and meningitis probably caused by borrelia burgdorferi. opsonic ... | 1989 | 2502833 |
| comparison of culture, indirect immunofluorescence and dark-field microscopy for detection of spirochetes from ixodes ricinus ticks. | | 1989 | 2504604 |
| infected ticks feeding on persons in areas endemic for lyme disease and rocky mountain spotted fever. | | 1989 | 2507649 |
| prevalence of antibody indicating lyme disease in farmers in wigtownshire. | | 1989 | 2510849 |
| clinical and microbiologic findings in six patients with erythema migrans of lyme disease. | the clinical course of six patients with erythema migrans of lyme disease was viewed in the context of antibiotic susceptibility studies of their own borrelia burgdorferi isolates. an initial poor response by two patients to antibiotic therapy suggested the possibility of b. burgdorferi resistance to these agents. minimum bactericidal concentration determinations of eight antimicrobial agents against these isolates did not support this suggestion. | 1989 | 2511231 |
| comparison of western blot and enzyme-linked immunosorbent assay for diagnosis of lyme borreliosis. | the usefulness of western blot in the serological diagnosis of lyme borreliosis was evaluated compared with an elisa using a whole cell sonicate antigen. fifty-three of 68 (78%) patients with neuroborreliosis had positive igm and/or igg immunoblots and 40 of 68 (59%) had positive igm and/or igg elisa titers in serum. eight of 44 (18%) controls with meningitis/encephalitis of non-borrelia etiology had positive igm and/or igg immunoblots and 4 of 44 (9%) had positive igm and/or igg elisa titers in ... | 1989 | 2512131 |
| prevalence of antibody indicating lyme disease in farmers in wigtownshire. | | 1989 | 2513072 |