| human chromosome 1 localization of the gene for a prostaglandin f2alpha receptor negative regulatory protein. | a protein that copurifies with the bovine prostaglandin f2alpha (fp) receptor has been isolated and the corresponding rat cdna has been cloned. transfection experiments suggest that this protein inhibits binding of [3h]prostaglandin f2alpha ([3h]pgf2alpha) to fp. histologically, this protein (fp regulatory protein or fprp) shows a distribution coinciding well with those cells and tissues that respond to pgf2alpha. a portion of the 3' untranslated region of the human homolog to fprp was subcloned ... | 1996 | 8655148 |
| analgesic and antipyretic activities of an aqueous extract and of the flavone linarin of buddleia cordata. | the dried aqueous extract of leaves of buddleia cordata (loganiaceae) and its main flavonoid glycoside, linarin, have been evaluated for analgesic and antipyretic effects in mice and rats, respectively. both the extract and linarin exerted significant and dose-dependent analgesic and antipyretic activities, the first being obtained against a chemical stimulus (writhing a test in mice) and the second being obtained by a pyretogenic stimulus (yeast-induced hyperthermia test). furthermore, the resp ... | 1996 | 8657746 |
| changes in superoxide anion production and phagocytosis by circulating neutrophils during tumor progression in a rat model. | the functional state of circulating neutrophils was monitored in a rat model of mesoblastic nephroma during tumor progression. superoxide anion (o2.-) production in response to pma and phagocytosis of yeast particles (saccharomyces cerevisiae) were measured every second day after tumor cell implantation. both phagocytosis and pma-induced 02.- generation were found to be enhanced in the first period (on days 6, 8, and 10), while they became significantly reduced in the advanced stage of cancer (o ... | 1996 | 8660819 |
| molecular characterization and expression of rat acyl-coa synthetase 3. | isolation and characterization of a rat brain cdna identified a third acyl-coa synthetase (acs) designated acs3. the deduced amino acid sequence of the cdna revealed that acs3 consists of 720 amino acids and exhibits a structural architecture common to acss from various origins. acs3 expressed in cos cells was purified to near homogeneity. the purified acs3 resolved by sds-polyacrylamide gel electrophoresis into two major proteins of 79 and 80 kda. cell-free translation of a synthetic mrna encod ... | 1996 | 8663269 |
| characterization of multiple mrnas that encode mammalian translation initiation factor 5 (eif-5). | eukaryotic translation initiation factor 5 (eif-5) interacts with the 40 s initiation complex (40s.mrna.mettrnaf.eif-2.gtp) to promote the hydrolysis of bound gtp with the concomitant joining of the 60 s ribosomal subunit to the 40 s initiation complex to form a functional 80 s initiation complex. in this paper, the multiple mrnas that encode mammalian eif-5 have been characterized. in rat tissues, three major eif-5 mrnas of 3.5, 2.8, and 2.2 kilobases in length are detected. all major eif-5 mrn ... | 1996 | 8663286 |
| mammalian sly1 regulates syntaxin 5 function in endoplasmic reticulum to golgi transport. | members of the syntaxin gene family are components of protein complexes which regulate vesicle docking and/or fusion during transport of cargo through the secretory pathway of eukaryotic cells. we have previously demonstrated that syntaxin 5 is specifically required for endoplasmic reticulum to golgi transport (dascher, c., matteson, j., and balch, w. e.(1994) j. biol. chem. 269, 29363-29366). to extend these observations we have now cloned a protein from rat liver membranes which forms a native ... | 1996 | 8663406 |
| tmp21 and p24a, two type i proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking. | we report here on the isolation, cloning, and expression of two mr 21,000 proteins from rat pancreatic acinar cells, the rat-tmp21 (transmembrane protein, mr 21,000) and the rat-p24a. both proteins are transmembrane proteins with type i topology and share weak but significant homology to one another (23% identity). we further show the cloning and characterization of the human homologs, hum-tmp21, which is expressed in two variants (tmp21-i and tmp21-ii), and hum-p24a. tmp21 proteins and p24a hav ... | 1996 | 8663407 |
| association of a dynamin-like protein with the golgi apparatus in mammalian cells. | dynamins are a family of 100-kd gtpases comprised of at least three distinct gene products and multiple alternatively spliced variants. homologies with the shibire gene product in drosophila melanogaster and with vps1p and dnm1p in saccharomyces cerevisiae suggest that dynamins play an important role in vesicular transport. morphological studies have localized brain dynamin to coated pits and tubular invaginations at the plasma membrane, where it is believed to facilitate the formation of endocy ... | 1996 | 8666662 |
| identifying a species-specific region of yeast tf11b in vivo. | the general transcription factor iib (tfiib) is required for rna polymerase ii transcription in eukaryotes. it provides a physical link between the tata-binding protein (tbp) and the rna polymerase and is a component previously suggested to respond to transcriptional activators in vitro. in this report, we compare the yeast (saccharomyces cerevisiae) and human forms of the protein in yeast cells to study their functional differences. we demonstrate that human tfiib fails to functionally replace ... | 1996 | 8668181 |
| difference in the mechanism of interaction of raf-1 and b-raf with h-ras. | ras is known to possess multiple cellular targets including raf-1. here, we measured both direct binding of various h-ras mutants to two representative mammalian ras targets, raf-1 and b-raf, and the activity of the mutants to stimulate raf-1 and b-raf, and analysed the difference in their ras-interaction mechanisms. b-raf was shown to share almost the same h-ras binding-specificity with raf-1 by examining binding of the h-ras mutants to raf-1 and b-raf in the yeast two-hybrid and in vitro bindi ... | 1996 | 8687465 |
| sequence and expression pattern of an evolutionarily conserved transcript identified by gene trapping. | we have isolated and analysed embryonic stem (es) cell clones after electroporation with a gene trap vector. clones were screened for changes in their lacz reporter gene activity upon in vitro differentiation. the cdna of one of the trapped transcripts, t10-2a2, was isolated and analysed in detail. although not expressed constitutively in differentiating es cells, the transcript was present in most organs of adult mice and widely expressed in midgestation mouse embryos. zoo blot analysis indicat ... | 1996 | 8688464 |
| cloning and characterization of complementary and genomic dnas encoding the epsilon-subunit of rat translation initiation factor-2b. | eukaryotic initiation factor-2b (eif-2b) is a guanine nucleotide-exchange protein involved in the recycling of eif-2 during peptide-chain initiation. regulation of eif-2b activity occurs under a wide range of conditions by diverse mechanisms. to better understand the regulation of eif-2b activity as well as the coordinate expression of its five subunits, we have begun to clone and characterize the cdnas and genes encoding these proteins. in the present study, complementary and genomic dnas encod ... | 1996 | 8688467 |
| kinesin-related proteins in the mammalian testes: candidate motors for meiosis and morphogenesis. | the kinesin superfamily of molecular motors comprises proteins that participate in a wide variety of motile events within the cell. members of this family share a highly homologous head domain responsible for force generation attached to a divergent tail domain thought to couple the motor domain to its target cargo. many kinesin-related proteins (krps) participate in spindle morphogenesis and chromosome movement in cell division. genetic analysis of mitotic krps in yeast and drosophila, as well ... | 1996 | 8688559 |
| the yeast and mammalian isoforms of phosphatidylinositol transfer protein can all restore phospholipase c-mediated inositol lipid signaling in cytosol-depleted rbl-2h3 and hl-60 cells. | the mammalian phosphatidylinositol transfer proteins (pitp) and the yeast saccharomyces cerevisiae pitp (sec14p) that show no sequence homology both catalyze exchange of phosphatidylinositol (pi) between membranes compartments in vitro. in hl-60 cells where the cytosolic proteins are depleted by permeabilization, exogenously added pitpalpha is required to restore g protein-mediated phospholipase cbeta (plcbeta) signaling. recently, a second mammalian pitpbeta form has been described that shows 7 ... | 1996 | 8692861 |
| cloning, expression pattern and mapping to 12p 13.2 --> p13.1 of claps3, a gene encoding a novel clathrin-adaptor small chain. | from a human fetal-brain cdna library we isolated a novel gene encoding a peptide homologous to clathrin-adaptor small chains in rat, mouse, and yeast. the cdna, designated claps3 (clathrin-associated/assembly/adaptor protein, small 3, 22 kda), contained an open reading frame of 579 nucleotides encoding 193 amino acids. northern-blot analysis revealed expression of a 1.35-kb transcript in all human tissues examined. this gene was mapped to chromosome bands 12p13.2 --> p13.1 by fish. | 1996 | 8697810 |
| characterization of the reductase domain of rat neuronal nitric oxide synthase generated in the methylotrophic yeast pichia pastoris. calmodulin response is complete within the reductase domain itself. | rat neuronal no synthase (nnos) is comprised of a flavin-containing reductase domain and a heme-containing oxygenase domain. calmodulin binding to nnos increases the rate of electron transfer from nadph into its flavins, triggers electron transfer from flavins to the heme, activates no synthesis, and increases reduction of artificial electron acceptors such as cytochrome c. to investigate what role the reductase domain plays in calmodulin's activation of these functions, we overexpressed a form ... | 1996 | 8702805 |
| [antifebrile and anti-inflammatory effects of radix cynanchi atrati]. | the water extract of radix cynanchi atrati used as intraperitoneal injection has been proved to have an obvious antifebrile++ effect on rat fever caused by 15% yeast suspension hypodermic injection as well as a significant anti-inflammatory effect. but the antiferbrile effect of its ethanol extract is not clear. | 1995 | 8703343 |
| characterization of the interaction of the monomeric gtp-binding protein rab3a with geranylgeranyl transferase ii. | the monomeric gtp-binding protein rab3a controls exocytosis in neuroendocrine and neuronal cells. like other members of the rab family, rab3a is posttranslationally modified by the addition of hydrophobic geranylgeranyl groups to its c-terminus. the geranylgeranylation reaction is catalysed by the heterotrimeric geranylgeranyl transferase ii. we describe the cdna cloning of the beta-subunit of human geranylgeranyl transferase ii by means of the yeast two-hybrid system. the human enzyme, which is ... | 1996 | 8706741 |
| isolation and expression of a cdna clone encoding human kynureninase. | kynureninase (l-kynurenine hydrolase), a pyridoxal-5'-phosphate-(pyridoxal-p)-dependent enzyme, catalyses the cleavage of l-kynurenine and l-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. in this report, we describe the isolation of a cdna clone encoding human kynureninase. degenerate oligonucleotides designed from the amino acid sequences of peptides from rat liver kynureninase, were used as primers for reverse-transcription pcr of rat kidney rna. the resulti ... | 1996 | 8706755 |
| the human mitochondrial import receptor, htom20p, prevents a cryptic matrix targeting sequence from gaining access to the protein translocation machinery. | yeast mas70p and nadh cytochrome b5 reductase are bitopic integral proteins of the mitochondrial outer membrane and are inserted into the lipid-bilayer in an nin-ccyto orientation via an nh2-terminal signal-anchor sequence. the signal anchor of both proteins is comprised of a short, positively charged domain followed by the predicted transmembrane segment. the positively charged domain is capable of functioning independently as a matrix-targeting signal in yeast mitochondria in vitro but does no ... | 1996 | 8707817 |
| retinoid x receptor isotype identity directs human vitamin d receptor heterodimer transactivation from the 24-hydroxylase vitamin d response elements in yeast. | unlike estrogen and progesterone receptors that operate as homodimers on response elements, retinoid x receptors (rxrs) and vitamin d receptors (vdrs) can function as heterodimers. studies concerning the significance of heterodimeric partnerships are usually performed utilizing mammalian or insect cells. these cells express endogenous nuclear receptors, making it impossible to assign a role for one receptor subtype over another while studying the function of transfected receptor(s). yeast lacks ... | 1996 | 8721985 |
| myosin-v: a class of unconventional molecular motors. | in this review we focus on the biochemical and structural properties of the myosin-v class of unconventional myosins as an example of the diversity of molecular motors within the myosin superfamily. a member of this class was first identified as a novel calmodulin-binding protein in mammalian brain (larson re, pitta de and ferro ja (1988). brazilian journal of medical and biological research, 21: 213-217). to date, the myosin-v class is represented by two molecules from yeast, one from nematodes ... | 1996 | 8736123 |
| changes in the phosphorylation of eukaryotic initiation factor 2 alpha, initiation factor 2b activity and translational rates in primary neuronal cultures under different physiological growing conditions. | phosphorylation of the alpha-subunit of eukaryotic initiation factor 2 (eif-2) is one of the best known mechanisms regulating protein synthesis in a wide range of eukaryotic cells, from yeast to human. to determine whether this mechanism operates in primary neuronal cells, we have cultured primary neuronal cells for 7 days under two optimal growing conditions, complete medium (containing 15% serum) and serum-free medium, and determined the protein synthesis rate, eukaryotic initiation 2 and 2b ( ... | 1996 | 8737673 |
| mitochondrial dna migration events in yeast and humans: integration by a common end-joining mechanism and alternative perspectives on nucleotide substitution patterns. | in contrast to extensive infiltration of plant nuclear genomes by mitochondrial and chloroplast dna fragments, a computer assessment method could only detect seven mitochondrial dna integration events in saccharomyces cerevisiae chromosomes and five examples of dna migration into mammalian nuclear genes. no evidence could be detected for mitochondrial dna insertion into chromosome iii of caenorhabditis elegans or in nuclear dna sequences of drosophila sp. or plasmodium falciparum. thus, the quan ... | 1996 | 8742642 |
| cloning of brassica napus ctp: phosphocholine cytidylyltransferase cdnas by complementation in a yeast cct mutant. | ctp:phosphocholine cytidylyltransferase is a rate-limiting enzyme in biosynthesis of phosphatidylcholine in plant cells. we have isolated four cdnas for the cytidylyltransferase from a root cdna library of brassica napus by complementation in a yeast cct mutant. the deduced amino-acid sequences of the b. napus enzymes resembled rat and yeast enzymes in the central domain. although all cytidylyltransferases ever cloned from b. napus and other organisms were predicted to be structurally hydrophili ... | 1996 | 8756587 |
| the pou domain of scip/tst-1/oct-6 is sufficient for activation of an acetylcholine receptor promoter. | in the pc12 neuroendocrine line, the neuronal nicotinic acetylcholine receptor alpha3 gene promoter is activated by scip/tst-1/oct-6, a pou domain transcription factor proposed to be important for regulating the development of specific neural cell populations. in this study, we have investigated the scip polypeptide domains involved in alpha3 promoter activation. the characteristics of activation by a chimeric effector in which the gal4 dna binding domain was substituted for the scip pou domain ... | 1996 | 8756659 |
| molecular cloning of a rna binding protein, s1-1. | s1 proteins a-d constitute a nuclear protein family that are liberated rapidly in a set from chromatin by mild digestion with a dna or rna hydrolyzing enzyme. with an anti-s1-protein b antiserum that reacted with b2, c1 and d1, a cdna clone, ps1-1, was obtained, which encoded a protein of 852 amino acids. the s1-1 protein, encoded within the cells by a mrna of 3480 nt, was a novel protein and could be distinguished from the s1 proteins b, c and d by their amino acid sequences. the s-1-1 protein ... | 1996 | 8760884 |
| broadly expressed snt-like proteins link fgf receptor stimulation to activators of ras. | snt was originally described as a approximately 90 kilodalton protein in neuronal precursor cells which bears affinity for the yeast cell cycle protein p13sucl and which undergoes rapid tyrosine phosphorylation following stimulation with growth factors which trigger terminal differentiation, but not by other growth factors which promote proliferation (rabin et al., 1993). we show here that similarly sized snt-like proteins (slps) are expressed in fibroblast, myoblast, and lymphoid cell lines, an ... | 1996 | 8761293 |
| purification and functional characterization of type ii dna topoisomerase from rat testis and comparison with topoisomerase ii from liver. | a number of studies in yeast have shown that dna topoisomerase ii is essential for chromosome condensation and disjunction during mitosis at the metaphase/anaphase transition and meiosis i. accordingly, kinetic and mechanistic studies have implied a role for topoisomerase ii in chromosome disjunction. as a step toward understanding the nature and role of topoisomerase ii in a mammalian germline in vivo, we have purified topoisomerase ii from rat testis to homogeneity and ascertained several of i ... | 1996 | 8765751 |
| role of translation initiation factor eif-2b in the regulation of protein synthesis in mammalian cells. | | 1996 | 8768075 |
| high-level bacterial expression of mitochondrial transport proteins. | the inability to obtain abundant quantities of purified, functional membrane proteins has represented a significant impediment to the goal of obtaining high-resolution structural information. in this review, procedures are described which have been developed in this laboratory and enable the high-level bacterial expression and subsequent purification of functional mitochondrial citrate transport proteins from yeast and rat liver. the data that we have obtained using these procedures and related ... | 1996 | 8786237 |
| alveolar macrophage response to yeasts and inert particles. | interactions between alveolar macrophages (am) from rats and a yeast with relatively high pathogenicity (candida albicans), a yeast with low pathogenicity (saccharomyces cerevisiae) and an inert control particle (amorphous silica) of similar diameters, 3-4 microns, were studied. both yeasts were phagocytized significantly faster by am than were the control particles and c. albicans significantly faster than s. cerevisiae. quantitative nitroblue tetrazolium reduction by am reflecting their oxidat ... | 1996 | 8786466 |
| beta-catenin associates with the actin-bundling protein fascin in a noncadherin complex. | catenins were first characterized as linking the cytoplasmic domains of cadherin cell-cell adhesion molecules to the cortical actin cytoskeleton. in addition to their essential role in modulating cadherin adhesivity, catenins have more recently been indicated to participate in cell and developmental signaling pathways. beta-catenin, for example, associates directly with at least two receptor tyrosine kinases and transduces developmental signals within the wnt pathway. catenins also complex with ... | 1996 | 8794867 |
| phylogenetic, structural and functional characteristics of the na-k-cl cotransporter family. | bumetanide-sensitive na-k-cl cotransporters and thiazide-sensitive na-cl cotransporters comprise a family of integral membrane transport proteins, the na-k-cl cotransporter (nkcc) family. each of the members of this family is over 1,000 amino acids in length. we have multiply aligned the ten currently sequenced members of this family from human, rabbit, rodent, shark, flounder, moth, worm and yeast sources. phylogenetic analyses suggest the presence of at least six isoforms of these full length ... | 1996 | 8801348 |
| expression and characterization of human pancreatic preprocarboxypeptidase a1 and preprocarboxypeptidase a2. | we are investigating the potential utility of human carboxypeptidases a in antibody-directed enzyme prodrug therapy (adept). hybridization screening of a human pancreatic cdna library with cdna probes that encoded either rat carboxypeptidase a1 (rcpa1) or carboxypeptidase a2 (rcpa2) was used to clone the human prepro-cpa homologs. after expression of the respective pro-hcpa cdna in saccharomyces cerevisiae, the enzymes were purified to homogeneity by a combination of hydrophobic and ion-exchange ... | 1996 | 8806703 |
| expression of rat neuronal nitric oxide synthase in saccharomyces cerevisiae. | the rat neuronal nitric oxide synthase (nnos) cdna was expressed in saccharomyces cerevisiae under the control of a hybrid pgk/gal promoter, pal. galactose induction resulted in the production of a soluble 160 kda protein that was recognised by anti-neuronal nos antibody. nos activity was detected by the conversion of [3h]arginine to [3h]citrulline and required the addition of the cofactors, calmodulin and tetrahydrobiopterin. the activity was inhibited by the arginine analogues ng-nitro-l-argin ... | 1996 | 8818271 |
| the inhibition of antigen-induced eosinophilia and bronchoconstriction by cdp840, a novel stereo-selective inhibitor of phosphodiesterase type 4. | 1. the novel tri-aryl ethane cdp840, is a potent and selective inhibitor of cyclic amp phosphodiesterase type 4 (pde 4) extracted from tissues or recombinant pde 4 isoforms expressed in yeast (ic50s: 4-45 nm). cdp840 is stereo-selective since its s enantiomer (ct 1731) is 10-50 times less active against all forms of pde 4 tested while both enantiomers are inactive (ic50s: > 100 microm) against pde types 1, 2, 3 and 5. 2. oral administration of cdp840 caused a dose-dependent reduction of interleu ... | 1996 | 8818342 |
| signal transduction pathway on beta-glucans-triggered hydrogen peroxide production by murine peritoneal macrophages in vitro. | it has been reported that the biological activities of several beta-glucans vary due to differences in their physicochemical properties. in this study we investigated the ability of beta-glucans to trigger h2o2 production and activate signaling pathway on peritoneal macrophages. the most effective beta-glucan tested on h2o2 production was zymocel which was a particulate preparation from the yeast cell wall. in contrast, gel-forming beta-glucans which are known as immunoenhancers did not trigger ... | 1996 | 8820904 |
| in vitro decrease of rat-derived pneumocystis carinii attachment induced by human natural yeast killer toxin-like antiidiotypic candidacidal antibodies. | | 1996 | 8822831 |
| soluble factors required for nuclear protein import. | | 1995 | 8824445 |
| the dose-related effects of paracetamol on hyperalgesia and nociception in the rat. | 1. we have studied the effects of 3 low doses of paracetamol (25, 50 and 100 mg kg-1 p.o.) on inflammatory hyperalgesia, inflammatory oedema, and nociceptive thresholds in rats. 2. at the lower dose (25 mg kg-1), paracetamol reduces only central hyperalgesia. 3. at the doses of 50 and 100 mg kg-1, paracetamol reduces also peripheral hyperalgesia; moreover, it enhances nociceptive thresholds to a mechanical stimulus in the non-inflamed paws. 4. neither paw inflammatory oedema nor tail nociceptive ... | 1996 | 8825353 |
| pneumocystis carinii telomere repeats are composed of ttaggg and the subtelomeric sequence contains a gene encoding the major surface glycoprotein. | we have cloned a telomere and adjacent sequences from rat-derived pneumocystis carinii using the ability of foreign telomeres to complement a yeast artificial chromosome (yac) deficient by one telomere in saccharomyces cerevisiae. characterization of the cloned dna in the recombinant yac demonstrated that it was a chimera of two p. carinii sequences, namely a 13.5 kb fragment of mitochondrial dna and an 8.3 kb distal portion consisting of subtelomeric dna. the p. carinii telomere repeat was demo ... | 1996 | 8825773 |
| investigation of a choline phosphate synthesis pathway in streptococcus pneumoniae: evidence for choline phosphate cytidylyltransferase activity. | in this study we have demonstrated the activity of a choline phosphate cytidylyltransferase in cell free extracts of streptococcus pneumoniae. southern blot analysis of restricted s. pneumoniae genomic dna probed with the gene coding for the choline phosphate cytidylyltransferase of saccharomyces cerevisiae demonstrated that there is homology between the s. cerevisiae cct gene and genomic dna of s. pneumoniae. we believe that this enzyme is involved in the biosynthesis of the choline containing ... | 1996 | 8837483 |
| ott, a mouse x-linked multigene family expressed specifically during meiosis. | the tissue expression patterns of 10 mouse testis cdnas were analysed by rt-pcr to search for new mammalian meiotic genes. the homologue of the rat synaptonemal complex protein gene scp1 is expressed in embryonic ovary, adult brain and testis. one novel gene is stringently testis specific and another is expressed exclusively in testis and embryonic ovary. the latter clone is not expressed in the testes of adult sex-reversed mice which lack germ cells, and therefore represents a meiosis-specific ... | 1996 | 8842733 |
| pcr in diagnosis of pneumocystosis of rats. | polymerase chain reaction (pcr) is a powerful technique to detect scanty amount of dna from living organisms. the present study intended to develop specific primers for pcr diagnosis of pneumocystosis and to evaluate diagnostic efficacy by preparation of template dnas from invasive bal fluid and also to screen serum or blood as a non-invasive specimen. albino rats of wistar or fischer strains were experimentally infected by pneumocystis carinii. extracted dnas or cell lysates of their blood, bro ... | 1996 | 8843695 |
| chemical synthesis and purification of proteins: a methodology. | classical stepwise solid-phase peptide synthesis (spps) has been used successfully for the synthesis of proteins up to 150 residues in length, although usually with poor yields and homogeneity. the major limitation has been the inability to separate chromatographically similar deletion and truncated impurities from the target sequence. we have developed a highly effective protocol for stepwise spps and 'one-step' purification of small proteins. to demonstrate the effectiveness of the methodology ... | 1996 | 8844261 |
| hepatitis b virus transactivator protein, hbx, associates with the components of tfiih and stimulates the dna helicase activity of tfiih. | human hepatitis b virus genome encodes a protein, termed hbx, that is widely recognized as a transcriptional transactivator. while hbx does not directly bind cis-acting transcriptional control elements, it has been shown to associate with cellular proteins that bind dna. because hbx transactivated a large number of viral/cellular transcriptional control elements, we looked for its targets within the components of the basal transcriptional machinery. this search led to the identification of its i ... | 1996 | 8855220 |
| effect of suramin on the biological activity of the two isoforms of the rat 5 alpha-reductase. | | 1996 | 8870171 |
| molecular cloning of a cdna coding for gtp cyclohydrolase i from dictyostelium discoideum. | the gtp cyclohydrolase i (gtp-ch) gene of the cellular slime mould dictyostelium discoideum has been cloned and sequenced. the 855 bp cdna of this gene contains the open reading frame (orf) encoding 232 amino acids with a predicted molecular mass of approx. 26 kda. southern blot analysis indicated the presence of a single gene for gtp-ch in dictyostelium. pcr amplification of the orf from chromosomal dna and sequencing showed the existence of a 101 bp intron in the gtp-ch gene of dictyostelium d ... | 1996 | 8870645 |
| caprine homologue of rodent 5'-amp-activated protein kinase subunit and yeast snf4/cat3 is down-regulated by thyroid hormone. | we isolated a cdna, b12, that was down-regulated by thyroid hormone (th) in the goat cerebellum, using a polymerase chain reaction (pcr)-based subtractive hybridization and differential screening procedure. northern blot analysis of rna from cerebellum of t4-treated and untreated hypothyroid goats confirmed that clone b12 was th-regulated with an average reduction in expression of 21% after 4 days of t4 supplementation. other tissues from a t4-treated and an untreated hypothyroid goat also revea ... | 1996 | 8872308 |
| involvement of the alpha subunit of farnesyl-protein transferase in substrate recognition. | using photoaffinity labeling, we have identified a region in mammalian farnesyl-protein transferase (fptase) involved in substrate recognition. the photolabel used (compound 1) is a peptide containing the photoactive amino acid p-benzoylphenylalanine (bpa). upon exposure to uv light. compound 1 inhibits fptase activity in a time- and concentration-dependent manner. photoinhibition of fptase activity by compound 1 is prevented by adding h-ras to the reaction mixture, indicating that labeling is t ... | 1996 | 8873619 |
| the toxic milk mouse is a murine model of wilson disease. | wilson disease (wd) is an autosomal recessive defect of copper transport characterized by massive accumulation of copper in the liver, which can lead to liver failure. mutations in a copper transporting atpase (wnd or atp7b) have been shown to cause the disease. the toxic milk mouse mutant (tx) accumulates copper in the liver in a manner similar to that observed in patients with wd. however, some physiological differences between tx mice and human wd patients have cast doubts on whether this mut ... | 1996 | 8894697 |
| direct inhibition of the signaling functions of the mammalian target of rapamycin by the phosphoinositide 3-kinase inhibitors, wortmannin and ly294002. | the immunosuppressant, rapamycin, inhibits cell growth by interfering with the function of a novel kinase, termed mammalian target of rapamycin (mtor). the putative catalytic domain of mtor is similar to those of mammalian and yeast phosphatidylinositol (pi) 3-kinases. this study demonstrates that mtor is a component of a cytokine-triggered protein kinase cascade leading to the phosphorylation of the eukaryotic initiation factor-4e (eif-4e) binding protein, phas-1, in activated t lymphocytes. th ... | 1996 | 8895571 |
| tyrosine kinase activation is an immediate and essential step in hypotonic cell swelling-induced erk activation and c-fos gene expression in cardiac myocytes. | hypotonic stress causes rapid cell swelling and initiates various cellular adaptive processes. however, it is unknown how cells initially sense low osmolarity and convert it into intracellular signals. we investigated the signal transduction mechanism initiated by hypotonic cell swelling in cardiac myocytes using c-fos expression as a nuclear marker. treatment of myocytes with hypotonic culture media rapidly induced c-fos expression, whereas hypertonic stress had no effect. transfection of c-fos ... | 1996 | 8896447 |
| the product of the saccharomyces cerevisiae rss1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the rat7/nup159 nucleoporin, is required for efficient mrna export. | rat7/nup159 was identified previously in a screen for genes whose products are important for nucleocytoplasmic export of poly(a)+ rna and encodes an essential nucleoporin. we report here the identification of rss1 (rat seven suppressor) as a high-copy extragenic suppressor of the rat7-1 temperature-sensitive allele. rss1p encodes a novel essential protein of 538 amino acids, which contains an extended predicted coiled-coil domain and is located both at nuclear pore complexes (npcs) and in the cy ... | 1996 | 8898365 |
| acylation of glucosaminyl phosphatidylinositol revisited. palmitoyl-coa dependent palmitoylation of the inositol residue of a synthetic dioctanoyl glucosaminyl phosphatidylinositol by hamster membranes permits efficient mannosylation of the glucosamine residue. | two critical steps in the assembly of yeast and mammalian glycosylphosphatidylinositol (gpi) anchor precursors are palmitoylation of the inositol residue and mannosylation of the glucosamine residue of the glucosaminyl phosphatidylinositol (glcnalpha-pi) intermediate. palmitoylation has been reported to be acyl-coa dependent in yeast membranes (costello, l. c., and orlean, p. (1992) j. biol. chem. 267, 8599-8603) but strictly acyl-coa independent in rodent membranes (stevens, v. l., and zhang, h ... | 1996 | 8900192 |
| isoform-specific purification and substrate specificity of the 5'-amp-activated protein kinase. | the 5'-amp-activated protein kinase (ampk) mediates several cellular responses to metabolic stress. rat liver contains at least two isoforms of this enzyme, either alpha1 or alpha2 catalytic subunits together with beta and gamma noncatalytic subunits in a trimeric complex. the alpha1 isoform is purified using a peptide substrate affinity chromatography column with adr1 (222-234)p229 (lkkltrrpsfsaq), corresponding to the camp-dependent protein kinase phosphorylation site in the yeast transcriptio ... | 1996 | 8910470 |
| endoplasmic reticulum form of calreticulin modulates glucocorticoid-sensitive gene expression. | calreticulin is a ubiquitously expressed ca2+-binding protein of the endoplasmic reticulum (er), which inhibits dna binding in vitro and transcriptional activation in vivo by steroid hormone receptors. transient transfection assays were carried out to investigate the effects of different intracellular targeting of calreticulin on transactivation mediated by glucocorticoid receptor. bsc40 cells were transfected with either calreticulin expression vector (er form of calreticulin) or calreticulin e ... | 1996 | 8910610 |
| rat 17 beta-hydroxysteroid dehydrogenase type iv is a novel peroxisome proliferator-inducible gene. | to better understand the molecular mechanisms of the pleiotropic responses induced by exposure to peroxisome proliferator chemicals (ppcs), we conducted a systematic search for genes whose mrna levels are modulated by the ppc wy-14,643 (wy) in rat liver. the sequence of one up-regulated cdna (2480 bp) was predicted to encode a protein of 735 aa with 82% identity to the porcine 17 beta-hydroxysteroid dehydrogenase type iv (hsd iv). like the porcine enzyme, the rat hsd iv contains' a region homolo ... | 1996 | 8913347 |
| isolation of a brefeldin a-inhibited guanine nucleotide-exchange protein for adp ribosylation factor (arf) 1 and arf3 that contains a sec7-like domain. | brefeldin a (bfa) inhibited the exchange of adp ribosylation factor (arf)-bound gdp for gtp by a golgi-associated guanine nucleotide-exchange protein (gep) [helms, j.b. & rothman, j.e. (1992) nature (london) 360, 352-354; donaldson, j.g., finazzi, d. & klausner, r.d. (1992) nature (london) 360, 350-352]. cytosolic arf gep was also inhibited by bfa, but after purification from bovine brain and rat spleen, it was no longer bfa-sensitive [tsai, s.-c., adamik, r., moss, j. & vaughan, m. (1996) proc. ... | 1996 | 8917509 |
| cloning of sponge (geodia cydonium) and tunicate (botryllus schlosseri) proteasome subunit epsilon (prce): implications about the vertebrate mhc-encoded homologue lmp7 (prcc). | proteasomes are large protein complexes that play a major role in selective degradation of intracellular proteins. eukaryotes feature seven different alpha and beta subunits. two of the vertebrate housekeeping beta-subunits have mhc-encoded homologues that can substitute for the housekeeping counterparts upon interferon-gamma induction. in the present study we report the cloning of invertebrate beta-subunit proteasome epsilon (prce), from the marine sponge geodia cydonium and from the colonial t ... | 1996 | 8920927 |
| copii vesicles derived from mammalian endoplasmic reticulum microsomes recruit copi. | er to golgi transport requires the function of two distinct vesicle coat complexes, termed copi (coatomer) and copii, whose assembly is regulated by the small gtpases adp-ribosylation factor 1 (arf1) and sar1, respectively. to address their individual roles in transport, we have developed a new assay using mammalian microsomes that reconstitute the formation of er-derived vesicular carriers. vesicles released from the er were found to contain the cargo molecule vesicular stomatitis virus glycopr ... | 1996 | 8922375 |
| cellular immune response to retinal s-antigen & interphotoreceptor retinoid binding protein fragments in idiopathic human uveitis. | the role of retinal antigens in idiopathic human uveitis has been studied in 38 patients of uveitis, and 30 patients of systemic connective tissue disease (ctd) and 30 healthy volunteers. lymphocyte proliferative responses were tested in vitro against native s-antigen, its uveitopathogenic peptides (peptide m, peptide g), yeast histone h3 peptide and uveitopathogenic fragment of interphotoreceptor retinoid binding protein (irbp: r16) to establish their role in pathogenesis of human uveitis. seve ... | 1996 | 8935743 |
| molecular cloning of cdna encoding rat very long-chain acyl-coa synthetase. | the cdna encoding rat very long-chain acyl-coa synthetase (vlacs) was cloned, using degenerative primers synthesized according to the partial amino acid sequences of the peptide fragments of the purified rat liver enzyme. the longest cdna insert was 2972 base pairs with a 1860-base pair open reading frame encoding 620 amino acids. the calculated molecular mass of 70,692 daltons was consistent with size of the purified enzyme. in northern blot analysis, a single band was detected at the position ... | 1996 | 8939997 |
| the amino acid sequence of rat kidney 5-oxo-l-prolinase determined by cdna cloning. | 5-oxoprolinase (ec 3.5.2) catalyzes a reaction in which the endergonic cleavage of 5-oxo-l-proline to form l-glutamate is coupled to the exergonic hydrolysis of atp to adp and inorganic phosphate. highly purified preparations of the enzyme have been obtained from rat kidney and pseudomonas putida. the rat kidney enzyme is composed of two strongly interacting, apparently identical subunits (mr = 142,000), whereas that from p. putida is composed of two functionally different protein components tha ... | 1996 | 8943290 |
| the involvement of nitric oxide in the anti-candida albicans activity of rat neutrophils. | rat peritoneal neutrophils (pmn) spontaneously release nitric oxide (no) when incubated in vitro. addition of the no synthase inhibitor l-monomethylarginine (l-nmma) to the pmn reduces no production and impairs the killing of the yeast candida albicans, both effects being reversed by l-arginine. these data strongly suggest that oxidative metabolism of l-arginine by pmn is involved in the candidacidal activity of these cells. rat blood pmn, which do not produce significant amounts of no, exhibit ... | 1996 | 8943729 |
| carp cdna sequence encoding a putative diazepam-binding inhibitor/endozepine/acyl-coa-binding protein. | a full-length cdna coding for common carp diazepam-binding inhibitor (dbi)/endozepine (ep)/acyl-coa-binding protein (acbp) was isolated and sequenced. the deduced dbi/ep/acbp is comprised of 87 amino acids (including initiating methionine) without possessing a signal peptide. common carp dbi/ep/acbp displays 77%, 78%, 70%, 63%, 61% and 45% identity with human, bovine, rat, frog, duck and yeast dbi/ep/acbp, respectively. | 1996 | 8948484 |
| self-interaction of the major 27-kilodalton outer dense fiber protein is in part mediated by a leucine zipper domain in the rat. | the rt7 gene is exclusively expressed in spermatids and encodes the 27-kda major outer dense fiber (odf) protein odf27. analysis of its amino acid structure had indicated the presence of a putative leucine zipper dimerization motif in the n-terminus and the presence of pcx repeats in the c-terminus. we had previously shown that the odf27 n-terminal fragment can interact with full-length odf27. we have used two different methods to analyze this interaction further. first we used fusion proteins b ... | 1996 | 8949892 |
| molecular cloning and expression of a cdna encoding a protein tyrosine phosphatase from mouse brain. | a mouse brain cdna library was screened with a radioisotope-labeled probe of human placental protein tyrosine phosphatase. the isolated clone (mbptp1b) was found to contain an open reading frame of 1,296 nucleotides as well as 5' (709 nucleotides) and 3' (341 nucleotides) non-coding regions. this cdna encodes a ptp of 432 amino acids having a mass of 49,563 daltons and exhibiting 83% and 93.5% sequence identity to that of human ptp1b and rat ptp1, respectively. the expression of the cdna in yeas ... | 1996 | 8955893 |
| uncoupling of dna replication and cell cycle progression by human cyclin e. | the g1-specific d- and e-type cyclins are among the most crucial factors controlling cell cycle progression in mammalian cells and are therefore thought to play an important role in tumorigenisis. d-type cyclins have indeed been shown to be endowed with an oncogenic potential. here, we report that the ectopic expression of human cyclin e, but not cyclin d1, deregulates dna synthesis in both yeast and mammalian cells. in yeast, induction of dna synthesis by cyclin e occurs even under conditions o ... | 1996 | 8957094 |
| engineering the c-region of human insulin-like growth factor-1: implications for receptor binding. | recombinant wild-type human igf-1 and a c-region mutant in which residues 28-37 have been replaced by a 4-glycine bridge (4-gly igf-1) were secreted and purified from yeast. an igf-1 analogue in which residues 29-41 of the c-region have been deleted (mini igf-1) was created by site-directed mutagenesis and also expressed. all three proteins adopted the insulin-fold as determined by circular dichroism. the significantly raised expression levels of mini igf-1 allowed the recording of two-dimension ... | 1996 | 8961354 |
| [effectiveness of the use of nutrient media in isolation of yersinia]. | three liquid nutrient media (phosphate buffer medium, 1% buffered peptone water ph 7.6-7.7, buffer casein yeast medium) and live solid media (endo's, serov's, and differential diagnostic medium with bromothymol blue, made in this country, and two foreign ones, agar deoxycholate and mcconkie's) are compared in examinations of the material from 213 patients with acute intestinal diseases and from 460 small mammals. the results demonstrate a higher efficacy of bacteriologic investigations carried o ... | 1993 | 8963546 |
| removal of an intron with unique 3' branch site creates an amino-terminal protein sequence directing the scerv1 gene product to mitochondria. | the yeast scerv1 gene is of special interest because it has a dual function in mitochondrial biogenesis and in the regulation of the cell cycle. the recent discovery that the yeast scerv1 gene has a structural and functional human homologue initiated a detailed comparison of the genes and their structures. in addition the homologous alr (augmenter of liver regeneration) genes from rat and mouse have just been identified and it has been found that the mammalian proteins have a specific function i ... | 1996 | 8972573 |
| investigation of the anti-inflammatory activity of acacia nilotica and hibiscus sabdariffa. | the aqueous extracts of acacia nilotica and hibiscus sabdariffa were tested for anti-inflammatory, analgesic and antipyretic activities in animal models. acacia nilotica extract had an inhibitory effect on carrageenan induced paw edema and yeast-induced pyrexia in rats. it also produced a significant increase in the hot plate reaction time in mice. hibiscus sabdariffa extract had no effect on paw edema but had an inhibitory effect on yeast induced pyrexia and a significant effect on the hot plat ... | 1996 | 8982438 |
| further study on association of 5srrna-l5 protein complex and methionyl-trna to methionyl-trna synthetase in the macromolecular aminoacyl-trna synthetase complex. | to obtain direct evidence for the attachment of 5srrna-ribosomal l5 protein particles (5srnp) and methionine-trna (trna(met)) to methionyl-trna synthetase (metrs) in the macromolecular aminoacyl-trna synthetase (ars) complex of rat liver, a metrs-5srnp-trna(met) complex was dissociated from the macromolecular ars complex fraction by n-octyl-beta-d-glucoside (method i) or by omega-aminooctyl agarose (method ii) chromatography. the dissociated metrs complex fraction was purified by gel filtration ... | 1996 | 8982850 |
| co-elevation of brain natriuretic peptide and proprotein-processing endoprotease furin after myocardial infarction in rats. | we investigated the expression of the yeast kex2 family endoproteases furin and pace4, and brain natriuretic peptide (bnp) in the atrium and ventricle after infarction as well as the conversion of the bnp precursor gammabnp to bnp-45. in a rat heart failure model, plasma bnp rose in two phases--first at day 3, and again at day 14. bnp mrna, as measured by northern blot analysis, increased strongly at day 3, then at days 14 and 28 less strongly in the atrium, and in the ventricle it increased wea ... | 1997 | 9001393 |
| tropomyosin isoforms in nonmuscle cells. | vertebrate nonmuscle cells, such as human and rat fibroblasts, express multiple isoforms of tropomyosin, which are generated from four different genes and a combination of alternative promoter activities and alternative splicing. the amino acid variability among these isoforms is primarily restricted to three alternatively spliced exon regions; an amino-terminal region, an internal exon, and a carboxyl-terminal exon. recent evidence reveals that these variable exon regions encode amino acid sequ ... | 1997 | 9002235 |
| peroxisomal multifunctional enzyme of beta-oxidation metabolizing d-3-hydroxyacyl-coa esters in rat liver: molecular cloning, expression and characterization. | in the present study we have cloned and characterized a novel rat peroxisomal multifunctional enzyme (mfe) named permfe-ii. the purified 2-enoyl-coa hydratase 2 with an m(r) of 31500 from rat liver [malila, siivari, mäkelä, jalonen, latipää, kunau and hiltunen (1993) j. biol. chem. 268, 21578-21585] was subjected to tryptic fragmentation and the resulting peptides were isolated and sequenced. surprisingly, the full-length cdna, amplified by pcr, had an open reading frame of 2205 bp encoding a po ... | 1997 | 9003397 |
| novel isoform of syntaxin 1 is expressed in mammalian cells. | syntaxin 1a has been identified previously as a neural-cell-specific, membrane-anchored receptor protein required for docking and fusion of synaptic vesicles with the presynaptic plasma membrane. syntaxin 1a consists of 288 amino acid residues including a 265-residue n-terminal region exposed to the cytoplasm and a c-terminal hydrophobic stretch of 23 residues believed to anchor syntaxin to the plasma membrane. using a human fat-cell library we have isolated a novel cdna clone of syntaxin 1a con ... | 1997 | 9003414 |
| anti-recombinogenic and convertible co-mutagenic effects of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) and other 5-substituted pyrimidine nucleoside analogs in s. cerevisiae mp1. | in experiments using yeast, without addition of an external metabolic activation system, (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) was co-mutagenic and showed an insignificant anti-recombinogenic effect in combination with triethylene melamine (tem). in the presence of activating s9-mix, the anti-recombinogenicity and co-mutagenicity could clearly be seen. at higher concentrations the co-mutagenic effect was converted into anti-mutagenicity. the other three 5-substituted pyrimidine nucleoside ... | 1996 | 9003540 |
| hexokinase as a sugar sensor in higher plants. | the mechanisms by which higher plants recognize and respond to sugars are largely unknown. here, we present evidence that the first enzyme in the hexose assimilation pathway, hexokinase (hxk), acts as a sensor for plant sugar responses. transgenic arabidopsis plants expressing antisense hexokinase (athxk) genes are sugar hyposensitive, whereas plants overexpressing athxk are sugar hypersensitive. the transgenic plants exhibited a wide spectrum of altered sugar responses in seedling development a ... | 1997 | 9014361 |
| cytochrome p450 2c11: escherichia coli expression, purification, functional characterization, and mechanism-based inactivation of the enzyme. | the male-specific p450 enzyme cyp 2c11, whose expression is developmentally and hormonally regulated, is the major steroid 16alpha-hydroxylase of the untreated rat liver. the enzyme metabolizes a host of substrates, including mechanism-based inactivators, such as 3,5-dicarbethoxy-2,6-dimethyl-4-ethyl-1,4-dihydropyridine (ddep) and spironolactone (spl). structural and functional characterization of the specific mode of such inactivation, however, requires sufficient quantities of the fully purifi ... | 1997 | 9015385 |
| the cytoplasmic domain of rat nkr-p1 receptor interacts with the n-terminal domain of p56(lck) via cysteine residues. | nkr-p1 is a type ii transmembrane protein which acts as an activation receptor on natural killer (nk) cells. the cytoplasmic domains of the cd4, cd8 and 4-1bb receptors contain the sequence cys-x-cys-pro which is directly involved in coupling to another pair of cysteines in the n-terminal domain of the src family tyrosine kinase p56(lck). the cytoplasmic domain of nkr-p1 in rodents also contains the cys-x-cys-pro sequence, but the capacity of the receptor to bind p56(lck) is presently unknown. w ... | 1997 | 9022000 |
| acetaldehyde as well as ethanol is metabolized by human cyp2e1. | acetaldehyde was oxidized by rat and human hepatic microsomes in the presence of nadph. we designated this nadph-dependent oxidation system maos (microsomal acetaldehyde-oxidizing system), to distinguish it from the nad-dependent acetaldehyde oxidation system of acetaldehyde dehydrogenase in mitochondria and cytosol. this activity was increased 2.3-fold by giving rats ethanol. judging from the vmax/km values, the metabolic capacity of rat hepatic microsomes for maos activity was increased 24-fol ... | 1997 | 9023260 |
| isolation and characterization of glycogen synthase in dictyostelium discoideum. | we have partially purified the protein and isolated the glcs gene for glycogen synthase in dictyostelium. glcs mrna is present throughout development and is the product of a single gene coding for 775 amino acids, with a predicted molecular mass of 87 kd. the sequence is highly similar to glycogen synthase from human muscle, yeast, and rat liver, diverging significantly only at the amino and carboxy termini. phosphorylation and udpg binding sites are conserved, with k(m) values for udpg being co ... | 1996 | 9023987 |
| the multiple murine 3 beta-hydroxysteroid dehydrogenase isoforms: structure, function, and tissue- and developmentally specific expression. | the enzyme 3 beta-hydroxysteroid dehydrogenase (3 beta-hsd) is essential for the biosynthesis of all active steroid hormones. to date five distinct isoforms have been identified in the mouse. the different isoforms are indicated by roman numerals (i-v) in the chronological order in which they have been isolated. the different isoforms are expressed in a tissue- and developmentally specific manner and fall into two functionally distinct groups. 3 beta-hsd i, ii, and iii function as nad(+)-depende ... | 1997 | 9029733 |
| reflections on internal images. | | 1997 | 9035130 |
| therapeutic potential of antiidiotypic single chain antibodies with yeast killer toxin activity. | single chain fragment (scfv) antiidiotypic antibodies (antilds) of a killer toxin (kt) from the yeast pichia anomala have been produced by recombinant dna methodology from the splenic lymphocytes of mice immunized by idiotypic vaccination with a kt-neutralizing monoclonal antibody (mab kt4). scfv kt-like antilds (ktidab) react with specific candida albicans kt cell wall receptors (ktr) exerting a candidacidal activity in vitro could be neutralized by adsorption with mab kt4. scfv ktidab displaye ... | 1997 | 9035141 |
| biochemical and genetic interactions of two commercial pesticides with the monooxygenase system and chlorophyllin. | two commercial preparations of atrazine and zineb were tested on a diploid d7 strain of the yeast saccharomyces cerevisiae using cells from logarithmic growth phase (with a high level of cytochrome p-450) and from stationary growth phase. the compounds induced marked increases of both gene conversion and point mutation frequencies in the logarithmic phase cells, while in the stationary phase no genotoxic effect was observed. the results obtained employing ta98 and ta100 strains of salmonella typ ... | 1996 | 9037261 |
| central noradrenergic & dopaminergic modulation of brewer's yeast-induced inflammation & nociception in rats. | the possibility of central noradrenergic and dopaminergic modulation of brewer's yeast-induced peripheral inflammation was investigated in rats. centrally administered noradrenaline (na), amphetamine, which liberates na and dopamine in the central nervous system and l-dopa, the precursor of dopamine significantly suppressed paw oedema. conversely, the beta-adrenoceptor blocker, propranolol, catecholaminergic neuron degenerator, 6-hydroxydopamine (6-ohda), dopaminergic antagonist, haloperidol and ... | 1997 | 9055502 |
| phospholipase c isoforms delta 1 and delta 3 from human fibroblasts. high-yield expression in escherichia coli, simple purification, and properties. | phospholipase c isoforms delta 1 and delta 3 (plc delta 1 and delta 3) were expressed in escherichia coli using the cdna sequences from human fibroblasts. the enzymes were also expressed with the sequence met-gly-his6-ser-gly-leu-phe-lys-arg, a hexahistidine sequence followed by a kex2 protease cleavage site, denoted as "-h6k2," attached to their amino termini. plc delta 1, plc delta 1-h6k2, plc delta 3, plc delta 3-h6k2 all expressed in highly active form. the h6k2-bearing isoforms were each pu ... | 1997 | 9056492 |
| human succinic semialdehyde dehydrogenase. molecular cloning and chromosomal localization. | | 1997 | 9059628 |
| structural and mechanistic aspects of a new family of dehydrogenases, the beta-hydroxyacid dehydrogenases. | | 1997 | 9059644 |
| the s3a ribosomal protein gene is identical to the fte-1 (v-fos transformation effector) gene and the tnf-alpha-induced tu-11 gene, and its transcript level is altered in transformed and tumor cells. | previous work on mouse x rat hybrid cells (bs series) led to the assignment of a transformation suppressor locus (sail) to the rat 5q22-q33 region. this gene is not yet identified. from a non-transformed bs hybrid cell line, we isolated a partial cdna insert (13t), which detects a transcript more abundant in transformed cells than in their non-transformed homologs. sequence comparisons led us to conclude that 13t is identical to the coding sequences of the ribosomal protein s3a gene (rps3a), of ... | 1997 | 9074506 |
| influence of diet on the kinetic behavior of hepatic carnitine palmitoyltransferase i toward different acyl coa esters. | the influence of diet on the kinetics of the overt form of rat liver mitochondrial carnitine palmitoyltransferase (cpt i; ec 2.3.1.21) was studied using rats fed either a low-fat diet (3% w/w fat), or diets which were supplemented with either olive oil (oo), safflower oil (so) or menhaden (fish) oil (mo) to 20% w/w of fat (high fat diets). when animals were fed each of these four diets for 10 days, the order of the apparent maximal activity (vmax) of cpt i toward various individual fatty acyl co ... | 1997 | 9075190 |
| estrogenic activity of a dieldrin/toxaphene mixture in the mouse uterus, mcf-7 human breast cancer cells, and yeast-based estrogen receptor assays: no apparent synergism. | the estrogenic activity of dieldrin, toxaphene, and an equimolar mixture of both compounds (dieldrin/toxaphene) was investigated in the 21-day-old b6c3f1 mouse uterus, mcf-7 human breast cancer cells, and in yeast-based reporter gene assays. treatment of the animals with 17beta-estradiol (e2) (0.0053 kg/day x3) resulted in a 3.1-, 4.8-, and 7.8-fold increase in uterine wet weight, peroxidase activity, and progesterone receptor binding, respectively. in contrast, treatment with 2.5, 15 and 60 mic ... | 1997 | 9075711 |
| molecular cloning of mouse liver flavin containing monooxygenase (fmo1) cdna and characterization of the expression product: metabolism of the neurotoxin, 1,2,3,4-tetrahydroisoquinoline (tiq). | a mouse liver cdna clone, mfmo1, coding for a flavin-containing monooxygenase (fmo) was isolated. this cdna clone encoded a protein of 532 amino acids. based upon its predicted amino acid sequence, this clone was assumed to belong to the fmo1 subfamily. the deduced amino acid sequence showed 94, 84, 83, and 83% identity with fmo1s of rats, pigs, rabbits and humans, respectively, while it showed only 50-59% identity with human fmo3 and fmo4, rabbit fmo2, fmo3, fmo4 and fmo5, and guinea-pig fmo2. ... | 1997 | 9076656 |
| the molecular interaction of fas and fap-1. a tripeptide blocker of human fas interaction with fap-1 promotes fas-induced apoptosis. | fas (apo-1/cd95), which is a member of the tumor necrosis factor receptor superfamily, is a cell surface receptor that induces apoptosis. a protein tyrosine phosphatase, fas-associated phosphatase-1 (fap-1), that was previously identified as a fas binding protein interacts with the c-terminal 15 amino acids of the regulatory domain of the fas receptor. to identify the minimal region of the fas c-terminal necessary for binding to fap-1, we employed an in vitro inhibition assay of fas/fap-1 bindin ... | 1997 | 9079683 |
| cloning of a human cdna for ctp-phosphoethanolamine cytidylyltransferase by complementation in vivo of a yeast mutant. | ctp-phosphoethanolamine cytidylyltransferase (et) is the enzyme that catalyzes the formation of cdp-ethanolamine in the phosphatidylethanolamine biosynthetic pathway from ethanolamine. we constructed a saccharomyces cerevisiae mutant of which the ect1 gene, putatively encoding et, was disrupted. this mutant showed a growth defect on ethanolamine-containing medium and a decrease of et activity. a cdna clone was isolated from a human glioblastoma cdna expression library by complementation of the y ... | 1997 | 9083101 |
| cloning of ctp:phosphocholine cytidylyltransferase cdna from arabidopsis thaliana. | as one of the first steps to elucidate the relationship between the structure and function of ctp:phosphocholine cytidylytransferase (ec 2.7.7.15) in plants, the cytidylyltransferase cdna of arabidopsis thaliana was cloned and characterized. the a. thaliana cytidylyltransferase cdna is 1447 bp long and contains an open reading frame of 993 bp coding for a protein of 331 amino acids. the deduced structure of the enzyme was composed of three main regions; the catalytic domain in the n-terminal hal ... | 1997 | 9085266 |