| altered immune response to glycine-rich sequences of epstein-barr nuclear antigen-1 in patients with rheumatoid arthritis and systemic lupus erythematosus. | prior studies have shown that patients with rheumatoid arthritis (ra) have an increased number of circulating epstein-barr virus-infected b lymphocytes and elevated titers of antibody to epstein-barr nuclear antigen-1 (ebna-1), the major nuclear antigen expressed in latently infected b cells. however, it is not known whether antibodies from ra patients recognize the same epitopes as antibodies from normal subjects. are directed at the glycine-alanine repeating region of the molecule. antibodies ... | 1990 | 2164400 |
| high incidence of epstein-barr virus genomes in hodgkin's disease. | tissue specimens from 198 cases of hodgkin's disease and 151 non-hodgkin lymphomas, as well as 34 nonmalignant lymph node biopsies were examined for the presence of epstein-barr virus (ebv) dna by polymerase chain reaction. epstein-barr virus-specific dna sequences were detected in dna extracts from frozen and/or paraffin-embedded tissues of 58% of hodgkin's disease cases. high and low grade non-hodgkin lymphomas as well as chronic lymphocytic leukemia biopsies contained ebv dna in 26%, 14%, and ... | 1990 | 2164775 |
| deficiencies of human c3 complement receptors type 1 (cr1, cd35) and type 2 (cr2, cd21). | cr1 (cd35) and cr2 (cd21) are structurally related integral transmembrane glycoproteins that function as cellular receptors for human c3b and c3dg, respectively. the primary sequence of the most common structural allotype of cr1 and that of cr2 have been established, and ligand binding on the molecules has been mapped. cr1 and cr2 genes are located in close vicinity in the rca locus of chromosome 1. cr1 has a wide cellular/tissular distribution and mediates a variety of biologic functions, inclu ... | 1990 | 2164822 |
| cell surface alteration in epstein-barr virus-transformed cells from patients with extreme insulin resistance. | an abnormality was detected in the morphology of the cell surface of epstein-barr virus-transformed lymphocytes of patients with genetic forms of insulin resistance. in cells from two patients with leprechaunism and two patients with type a extreme insulin resistance, scanning electron microscopy demonstrated a decrease in the percentage of the cell surface occupied by microvilli in cells from the patients with leprechaunism and type a insulin resistance compared with control cells. when cells f ... | 1990 | 2165004 |
| a monoclonal antibody that neutralizes epstein-barr virus dna polymerase activity. | an epstein-barr virus (ebv) specific monoclonal antibody (mab), designated 55h3, was produced after immunizing balb/c mice with 12-o-tetradecanoylphorbol-13-acetate (tpa) activated b95-8 cells. it was possible to demonstrate that this mab detected an ebv-specific antigen(s) in the ebv genome-positive producer cell lines b95-8 and m-aba by immunofluorescence. immunoglobulin prepared from ascites fluid neutralized the activity of the ebv-encoded dna polymerase, but not the alkaline dnase. by weste ... | 1990 | 2165046 |
| different epstein-barr virus-b cell interactions in phenotypically distinct clones of a burkitt's lymphoma cell line. | epstein-barr virus (ebv)-positive burkitt's lymphoma (bl) biopsy cells and early passage bl cell lines have been reported as showing an unusual type of virus-cell interaction; at least two ebv latent proteins appear not to be expressed. serial passage of such lines is often accompanied by a broadening of virus latent gene expression and a corresponding change in the cell surface/growth phenotype towards that shown by in vitro transformed lymphoblastoid cell lines (lcls). the sequence of events, ... | 1990 | 2165133 |
| influence of chain length of pyrene fatty acids on their uptake and metabolism by epstein-barr-virus-transformed lymphoid cell lines from a patient with multisystemic lipid storage myopathy and from control subjects. | the uptake and intracellular metabolism of 4-(1-pyrene)butanoic acid (p4), 10-(1-pyrene)decanoic acid (p10) and 12-(1-pyrene)dodecanoic acid (p12) were investigated in cultured lymphoid cell lines from normal individuals and from a patient with multisystemic lipid storage myopathy (mlsm). the cellular uptake was shown to be dependent on the fatty-acid chain length, but no significant difference in the uptake of pyrene fatty acids was observed between mlsm and control lymphoid cells. after incuba ... | 1990 | 2165389 |
| classification of patients with common variable immunodeficiency by b cell secretion of igm and igg in response to anti-igm and interleukin-2. | we have classified patients with common variable immunodeficiency (cvi) on the basis of the ability of their b cells to respond to anti-igm and interleukin (il)-2 in vitro. group a had cells unable to secrete igm or igg, group b secreted igm alone, and group c secreted both igm and igg. a separate small group of patients lacked peripheral b cells. where ig secretion was present with anti-igm and il-2, ebv increased it, but where it was absent, ebv only induced igm secretion in two out of eight g ... | 1990 | 2165880 |
| the antiperinuclear factor. ii. variability of the perinuclear antigen. | since the antiperinuclear factor (apf) test on human buccal cells is rather unpredictable, we have investigated the possible factors determining the expression of appropriate antigens by the cells. we failed to find any relationship of the expression of perinuclear antigens to the donor's smoking habits, the degree of contamination with saprophytic bacteria, the presence or absence of blood group substances in saliva, or the titers of serum antibodies to epstein-barr virus. family studies were a ... | 1990 | 2165883 |
| epstein-barr virus types 1 and 2 differ in their ebna-3a, ebna-3b, and ebna-3c genes. | the two epstein-barr virus (ebv) types, ebv-1 and ebv-2, are known to differ in their ebna-2 genes, which are 64 and 53% identical in their nucleotide and predicted amino acid sequences, respectively. restriction endonuclease maps and serologic analyses detect few other differences between ebv-1 and ebv-2 except in the ebna-3 gene family. we determined the dna sequence of the ag876 ebv-2 ebna-3 coding region and have compared it with known b95-8 ebv-1 ebna-3 sequences to delineate the extent of ... | 1990 | 2166806 |
| contrasts in codon usage of latent versus productive genes of epstein-barr virus: data and hypotheses. | epstein-barr virus (ebv) has two different modes of existence: latent and productive. there are eight known genes expressed during latency (and hardly at all during the productive phase) and about 70 other ("productive") genes. it is shown that the ebv genes known to be expressed during latency display codon usage strikingly different from that of genes that are expressed during lytic growth. in particular, the percentage of s3 (g or c in codon site 3) is persistently lower (about 20%) in all la ... | 1990 | 2166815 |
| composite hodgkin's and non-hodgkin's lymphoma in a patient with acquired immune deficiency syndrome. in-situ demonstration of epstein-barr virus. | a 44-year-old man infected with human immunodeficiency virus had hodgkin's disease, mixed cellularity, and malignant non-hodgkin's lymphoma, diffuse large cell type. colorimetric in-situ hybridization showed the epstein-barr virus (ebv) genome in the cells of the large cell non-hodgkin's lymphoma and in the reed-sternberg cells and reactive lymphocytes of the hodgkin's lymphoma. these results suggest that ebv may play a similar causative role in both neoplasms. this colorimetric method of hybrid ... | 1990 | 2167145 |
| significance of viral coinfections by hiv, htlv-i, epstein-barr virus, and cytomegalovirus for immunological abnormalities in hemophiliacs. | in order to clarify the influence of viral coinfection on the immunological abnormalities of hemophiliacs infected with human immunodeficiency virus (hiv), 69 patients were examined. of the 69, 35 (50.7%) were hiv-antibody positive (hiv-ab+). two of them were acquired immunodeficiency syndrome (aids) and the remaining 33 were asymptomatic carriers (ac). anti-htlv-i was found in 6 of 43 (14.3%), and 2 of these 6 were also hiv-ab+. the frequencies of positive anti-viral capsid antigen (vca) igg ag ... | 1990 | 2167171 |
| detection of herpes viral genomes in normal and diseased corneal epithelium. | herpetic ocular disease is one of the major causes of corneal blindness. clinical diagnosis of corneal disease is based principally on corneal appearance. however, abnormal morphology of the corneal epithelium (ce) is not an indicator for the presence of a herpes virus. further, it has not been established if herpes viruses are present in normal corneal epithelial tissue. in these studies, the polymerase chain reaction was used to evaluate normal and diseased corneal epithelium for the presence ... | 1990 | 2167192 |
| type-c virus-like particles in a human b-cell lymphoma cell line. | type-c virus-like particles (vlps) were found in an epstein-barr (eb) virus-infected human b-cell lymphoma cell line, sp-50b, that was established from a patient with non-hodgkin lymphoma. the cell line continuously produces a small number of type-c vlps, 150-200 nm in diameter, over 1 year. sp-50b cells were negative for htlv-i and htlv-ii antigens and did not contain the htlv-i genome. in addition, two eb virus nuclear antigen (ebna)-positive b-cell lines, sp-54-cord and sp-57-cll, were establ ... | 1990 | 2167609 |
| comparison of the classical immunofluorescent antibody tests for epstein-barr virus with commercially available test kits. | twenty reference sera with previously reported titers plus 10 normal seropositive and 10 seronegative sera were used to determine which assay for the detection of antibodies to epstein-barr virus should be utilized in our laboratory. by three commercially available test systems and by slides prepared in our laboratory, all techniques detected the same number of samples positive for igg antibodies to viral capsid antigen (vca) and epstein-barr virus-determined nuclear antigen (ebna), but the geom ... | 1990 | 2167958 |
| ribosome-lamella complexes observed in an epstein-barr virus-producing b-cell line, infected with human t-lymphotropic virus type i. | co-cultivation of peripheral blood mononuclear cells, from a 4-year-old boy with infectious mononucleosis, with a human t lymphotrophic virus type i (htlv-i)-producing t-cell line resulted in the establishment of an htlv-i infected epstein-barr virus (ebv)-producing b-cell line. ultrastructural examination revealed the presence of ribosome-lamella complexes (rlcs), in a small proportion of long-term cultured cells. evidence is presented of continuity between rlc and the rough endoplasmic reticul ... | 1990 | 2167960 |
| synthesis of digoxigenin-labelled dna probe by polymerase chain reaction: application to epstein-barr virus and chlamydia trachomatis. | | 1990 | 2168087 |
| purification of epstein-barr virus associated dnase with affinity chromatography of nasopharyngeal carcinoma patient serum. | epstein-barr virus associated dnase in the homogeneous form can be purified by chromatographys using ch-sepharose 4b column conjugated with nasopharyngeal carcinoma patient serum, dna cellulose and phosphocellulose in that sequence. the molecular weight of this enzyme is shown to be 51 kilo-daltons in silver-staining and immunostains. among various methods for keeping dnase activity, the addition of bsa and dialysis in glycerol or ethylene glycol immediately after the enzyme purification is sugg ... | 1990 | 2168313 |
| lymphokine-activated killer (lak) cells discriminate between epstein-barr virus (ebv)-positive burkitt's lymphoma cells. | we have generated in vitro lymphokine-activated killer (lak) cells from healthy donors by stimulating their mononuclear leukocytes with recombinant interleukin-2 (ril-2) (100 u/ml). after 6 days in culture, the lytic properties of the lak cells were analyzed in the 51cr-release assay by utilizing a target panel of 6 paired lines consisting of an epstein-barr virus (ebv)-positive burkitt's lymphoma (bl) cell line and an ebv-transformed lymphoblastoid cell line (lcl) from the same donor, the raji ... | 1990 | 2168343 |
| enhanced ptosis in fisher's syndrome after epstein-barr virus infection. | a 20-year-old woman presented with bilateral ptosis, total ophthalmoplegia, cerebellar symptoms, and hyporeflexia, indicating fisher's syndrome. she had been diagnosed with infectious mononucleosis 2 months previously. increased epstein-barr virus (ebv) titer was noted, and the epstein-barr virus-associated nuclear antigen (ebna) became positive during the clinical course. apparent light-near dissociation of the pupils was noted and accommodation was intact. during pharmacological tests with top ... | 1990 | 2168901 |
| [subclinical course of epstein-barr virus infection in infants with transient hepatitis]. | epstein-barr-virus-(ebv)-infections are well known to cause acute infectious mononucleosis in adolescents and young adults. however in early infancy and childhood these infections are usually mild and clinically inapparent. in the following we describe a case of a primary ebv-infection in infancy, which led to a transient hepatitis, identified by pathologically elevated liver enzymes. | 1990 | 2169026 |
| effect of il-6 on proliferation and ig production of human ebv-transformed cell lines in serum free culture media. | to optimalize growth and ig production of ebv transformed b cells for large scale tissue culture, we analyzed five stable monoclonal ebv-b cell lines for their responsiveness to interleukin (il)-6 in standard medium with 5% fcs and in several serum-free media. as we previously demonstrated these cell lines were found to be heterogeneous with regard to their production of il-1, il-6 and lymphotoxin. we could not demonstrate an effect of il-6 on proliferation on any of the cell lines in either sta ... | 1990 | 2169441 |
| assessment of rapid elisa test for detection of epstein-barr virus infection. | a rapid test for the detection of igm and igg epstein-barr nuclear antigen (ebna-1) has been extensively marketed. if igm to epstein-barr viral capsid antigen (ebv vca) is taken as evidence of current ebv infection, one observer detected 17 of 38 such samples and the other 22 of 38 as acute. the positive predictive value of the test was 63%, and the greatest difficulty was posed by the detection of igm ebv vca positive, heterophile antibody negative samples. significant false positive results we ... | 1990 | 2169485 |
| suppression of human immunodeficiency virus type 1 replication by human herpesvirus-6. | the pathogenesis of aids is complex and poorly understood despite intensive research efforts. one of the most puzzling aspects of the disease is the long interval between primary infection with human immunodeficiency virus (hiv) and the production of antiviral antibodies and onset of overt disease. probably the most important factor that determines the length of these intervals is the rate at which hiv replicates within the infected host. molecular studies have suggested that the replication of ... | 1990 | 2169498 |
| [amplification of dna sequences in epstein-barr virus and human immunodeficiency virus using dna polymerase from thermus thermophilus]. | using thermophilic dna-polymerase from thermus thermophilus we have amplified by polymerase chain reaction (pcr) specific dna sequences of epstein-barr virus (ebv) and human immunodeficiency virus (hiv). dna-polymerase from thermus thermophilus (the molecular mass of 80 to 86 kda) differs in its physico-chemical properties from dna-polymerase from the thermus acquaticus (the molecular mass of 62 to 68 kda). to amplify the specific ebv dna sequence oligonucleotide primers for the virus replicon r ... | 1990 | 2169583 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 40-1990. a 16-year-old boy with a seizure disorder and past meningitis, current hepatic failure, and free intraperitoneal air. | | 1990 | 2169588 |
| severe combined immunodeficiency in man with an absence of immunoglobulin gene rearrangements but normal t cell receptor assembly. | an autosomal recessive type of severe combined immunodeficiency disease (scid) was characterized by an absence of immunoglobulins (ig) in the serum and of ig+ lymphocytes in bone barrow (bm) and peripheral blood. in the bm cd10+/terminal deoxynucleotidyl transferase-positive lymphocytes were identified. epstein-barr virus-transformed b lymphoblastoid cell lines (blcl) obtained from bm and peripheral blood did not synthesize ig. the ig heavy and light chain gene complexes in the blcl had retained ... | 1990 | 2170148 |
| high frequency of epstein-barr virus genome detection in hodgkin's disease of hiv-positive patients. | lymph nodes obtained from 7 hiv-positive and 20 hiv-negative patients with hodgkin's disease were examined for the presence of epstein-barr virus antigens and genome. ebv antigens were observed in only 2 out of 20 hiv-negative patients, whereas lymph nodes of hiv-positive patients did not reveal evidence of ebv antigens. by in situ hybridization and southern blot analysis, ebv genome was found in 5 out of 7 hiv-positive patients; the ebv genome was detected in the nucleus of reed-sternberg and h ... | 1990 | 2170278 |
| concatameric replication of epstein-barr virus: structure of the termini in virus-producer and newly transformed cell lines. | the linear form of epstein-barr virus (ebv) dna has homologous direct tandem repeats of approximately 500 bp at each terminus (tr). after infection, ebv dna circularizes via the tr to form the intracellular episomal dna. to analyze the mechanism of the synthesis of linear dna through possible replicative intermediates, the terminal fragments were identified in the total intracellular dna and the covalently closed circular dna from a productively infected cell line after induction of replication ... | 1990 | 2170673 |
| [preliminary study of the possibility of a viral etiology in cervical cancer]. | the authors examined 10 patients with cervical cancer and 5 with condyloma acuminatum. the sera were collected pre- and postoperatively and checked up to the presence of c-reactive protein (crp), while the method of the complement fixation reaction (cfr) was used to determine the presence of antibodies against herpes adeno and eb virus. the technique of indirect immunofluorescence was used to determine the presence of the mentioned viruses in the maliguant tissue. eight preoperatively obtained s ... | 1990 | 2170776 |
| variable expression of epstein-barr virus genome as demonstrated by in situ hybridization in central nervous system lymphomas in immunocompromised patients. | in situ hybridization, using a biotinylated sequence from the internal repeat (ir1) region of epstein-barr virus (ebv), was performed on two well characterized ebv-infected cell lines, b95-8 (productively infected) and namalwa (latently infected), and on eighteen formalin-fixed paraffin-embedded primary central nervous system (cns) lymphomas. ten of the lymphomas were from immunocompetent patients, and eight were from immunocompromised patients (five had acquired immunodeficiency syndrome (aids) ... | 1990 | 2170968 |
| specific human alloantibody produced by epstein-barr virus transformation of infiltrating cells from a rejected kidney graft. | | 1990 | 2171168 |
| differences in the extent of activation of epstein-barr virus replicative gene expression among four nonproducer cell lines stably transformed by orip/bzlf1 plasmids. | lymphoid cell lines were established which stably carry the epstein-barr viral (ebv) bzlf1 gene on an extrachromosomal plasmid. these lines, which spontaneously synthesize the bzlf1 gene product, zebra, were examined for expression of ebv genes which were activated by zebra. cell lines which acquired orip plasmids without bzlf1 served as controls. the extent of activation differed among derivatives of four cell lines. x50-7 cells, which harbor a standard latent ebv, could be induced by zebra to ... | 1990 | 2171186 |
| development of an igm antibody capture test using labelled fusion protein as antigen for diagnosis of b19 human parvovirus infections. | a new anti-b19 igm elisa was developed using the antibody-capture principle. biotinylated fusion protein was used as antigen. the specificity of the test was analysed using sera igm positive to rubella, hepatitis b core antigen, cytomegalovirus and epstein barr virus as well with sera positive for rheumatoid factors, antinuclear antibodies and with sera from normal blood donors. the specificity of the test was 96.18%. one hundred serum samples were tested by the new elisa and the standard macria ... | 1990 | 2171482 |
| [studies on transforming functions of epstein-barr virus-specific proteins]. | epstein-barr virus (ebv) infects human b lymphocytes and efficiently transforms them into immortalized lymphoblasts. ebv-determined nuclear antigen (ebna) and ebv latent infection membrane protein (lmp) are expressed in latently infected, growth-transformed lymphoblasts. to elucidate the functions of ebna and lmp, clones of cells were established that stably expressed ebna-1, ebna-2, ebna-3a, ebna-leader protein (ebna-lp) or lmp, using gene transfer technique and the growth characteristics of th ... | 1990 | 2172130 |
| detection of ebv gene expression in reed-sternberg cells of hodgkin's disease. | ebv dna has been detected by southern blot hybridization in 20-25% of hodgkin's disease tumor specimens and localized to the reed-sternberg cells by in situ hybridization. in the present investigation we used a 3h-labelled eber i anti-sense rna for in situ hybridization of archival formalin-fixed paraffin-embedded hodgkin's disease specimens previously shown by southern blot hybridization to be ebv-positive. in 6 of 8 specimens neoplastic cells showed an intense signal in virtually all of the tu ... | 1990 | 2172169 |
| expression of the epstein-barr virus genome in a nasopharyngeal carcinoma epithelial tumor cell line. | an epithelial tumor cell line was recently established from a biopsy specimen of a nasopharyngeal carcinoma (npc), and designated hone-i. uncloned (parental) hone-i and hone-i clone (c)-40 cells were found to contain latent epstein-barr virus (ebv). expression of the latent ebv genome in hone-i c-40 cells has been examined. it was possible to detect a small percentage of cells spontaneously synthesizing ebv early antigen (ea) and virus capsid antigen (vca) by immunofluorescence (if). in addition ... | 1990 | 2172173 |
| use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery. | the polymerase chain reaction was adapted to the amplification of a herpes simplex virus (hsv) dna sequence, common to hsv types 1 and 2 (hsv-1, hsv-2). the amplified product was detectable by ethidium-bromide staining or southern hybridization of gels and by dot hybridization. the hsv polymerase chain reaction detected hsv dna in samples obtained from eight patients with genital lesions from which hsv-2 was isolated in tissue culture and from four patients with labial lesions from which hsv-1 w ... | 1990 | 2172392 |
| recurrence of kawasaki disease and epstein-barr virus infection. | | 1990 | 2172398 |
| nucleotide sequence of a cytomegalovirus single-stranded dna-binding protein gene: comparison with alpha- and gammaherpesvirus counterparts reveals conserved segments. | the genomic sequence encoding a cytomegalovirus strain colburn homologue (db129) of the herpes simplex virus major dna-binding protein (icp8) was determined. multiple alignments of the deduced db129 amino acid sequence and three alpha- and gammaherpesvirus homologues revealed that 56% of the amino acid residues identical in all four homologues are contained within 12 relatively conserved segments, which together constitute only 11.2% of the shortest aligned sequence. in light of published icp8 d ... | 1990 | 2172458 |
| primary brain lymphoma in the immunocompetent host: relation to epstein-barr virus. | epstein-barr virus has been implicated in the pathogenesis of primary brain lymphoma in patients with congenital or acquired immunodeficiency states. to examine its role in central nervous system (cns) lymphoma in the immunocompetent host, epstein-barr virologic studies were performed in six consecutive cases seen at our institutions. virus dna sequences were detected in only one of three tumor specimens studied by southern blot hybridization. serologic studies from the entire cohort disclosed p ... | 1990 | 2172960 |
| adult t-cell leukemia-derived factor/thioredoxin, produced by both human t-lymphotropic virus type i- and epstein-barr virus-transformed lymphocytes, acts as an autocrine growth factor and synergizes with interleukin 1 and interleukin 2. | interleukin 1 (il-1) has been obtained from the epstein-barr virus-infected b-lymphoblastoid cell line 3b6 and shown to be involved in autocrine growth of 3b6 b cells. independently, adult t-cell leukemia-derived factor (adf) was purified from human t-lymphotropic virus i-infected leukemic t-cell line (atl-2) and reported as an interleukin 2 (il-2) receptor-inducing factor. we have previously reported the same molecular mass, pi, and nh2-terminal amino acid sequence for both 3b6-derived il-1 and ... | 1990 | 2172979 |
| expression of interleukin-10 activity by epstein-barr virus protein bcrf1. | cytokine synthesis inhibitory factor (csif; interleukin-10), a product of mouse th2 t cell clones that inhibits synthesis of cytokines by mouse th1 t cell clones, exhibits extensive sequence similarity to an uncharacterized open reading frame in the epstein-barr virus bcrf1. recombinant bcrf1 protein mimics the activity of interleukin-10, suggesting that bcrf1 may have a role in the interaction of the virus with the host's immune system. | 1990 | 2173142 |
| low prevalence of antibody to human herpesvirus-6 (hhv-6) in kadazans. | the prevalence of antibody to human herpesvirus type 6 (hhv-6) and epstein-barr virus (ebv) viral capsid antigens (vca) were analysed in sera from kadazans of sabah, north borneo. at a serum dilution of 10, about 34% were positive for hhv-6 antibody but in contrast all 95 individuals studied were positive for ebv vca antibody. the study shows that hhv-6 and ebv infection occur independently. the low frequency of seropositive individuals in this community suggests that other than socioeconomic fa ... | 1990 | 2173152 |
| expression of the epstein-barr virus (ebv)-encoded membrane antigen (lmp) increases the stimulatory capacity of ebv-negative b lymphoma lines in allogeneic mixed lymphocyte cultures. | epstein-barr virus (ebv)-negative burkitt lymphoma (bl) lines are poor stimulators in allogeneic mixed lymphocyte cultures compared to ebv-transformed lymphoblastoid cell lines derived from the same individuals. we have previously shown that the stimulatory capacity of the tumor cells is increased after ebv conversion (avila-carino et al., int. j. cancer 1987. 40: 691). as a first step towards the identification of the viral gene product responsible for this change we have studied the influence ... | 1990 | 2173662 |
| antibody responses against polypeptide components of epstein-barr virus-induced early diffuse antigen in patients with connective tissue diseases. | the specific humoral response against polypeptide components of epstein-barr virus (ebv), the induced early diffuse antigen (ea-d), in patients with connective tissue diseases, including systemic lupus erythematosus (sle) and mixed connective tissue disease (mctd), was investigated by using the immunoblotting technique. the ea(d)-positive sera from patients with infectious mononucleosis (im), nasopharyngeal carcinoma (npc), immunocompromised patients (renal transplant recipients and patients wit ... | 1990 | 2173736 |
| the epstein-barr virus bzlf1 gene product activates the human immunodeficiency virus type 1 5' long terminal repeat. | the epstein-barr virus immediate-early gene product bzlf1 transactivates the human immunodeficiency virus type 1 (hiv-1) long terminal repeat (ltr). the bzlf1 gene product caused an 18-fold increase in beta-galactosidase activity from an hiv-1 ltr lacz expression vector, whereas the hiv-1 transactivator tat caused a 44-fold increase in beta-galactosidase activity. when cells were transfected with both bzlf1 (pebv-z) and tat (ptat3) expression vectors, as well as hiv-1 ltr lacz plasmid (plron), a ... | 1990 | 2173793 |
| epstein-barr virus small nuclear rnas are not expressed in permissively infected cells in aids-associated leukoplakia. | epstein-barr virus (ebv) dna structure and gene expression were analyzed in tissue specimens from oral hairy leukoplakia (hlp), a mucocutaneous lesion that develops in patients infected with human immunodeficiency virus (hiv). the structure of the terminal restriction enzyme fragments of ebv revealed that hlp is a permissive infection without a predominant, detectable population of ebv episomal dna. in rna preparations from this uniquely permissive infection, ebv replicative mrnas could be ident ... | 1990 | 2174165 |
| translational control by the epstein-barr virus small rna eber-1. reversal of the double-stranded rna-induced inhibition of protein synthesis in reticulocyte lysates. | a role for the epstein-barr virus small rna species eber-1 in the regulation of protein synthesis has been investigated in the reticulocyte-lysate cell-free translation system. recombinant eber-1 was synthesized by in vitro transcription of a plasmid containing the viral gene and purified by cf11-cellulose chromatography and ribonuclease iii treatment. when added to the reticulocyte lysate at 10-20 micrograms/ml or more, eber-1 prevents the inhibition of protein synthesis caused by low concentra ... | 1990 | 2174360 |
| expression of epstein-barr virus-encoded proteins and b-cell markers in fatal infectious mononucleosis. | we assessed 33 lymphoid tissues from 15 patients, including 7 with x-linked lymphoproliferative disease (xlp) and 8 patients with sporadic fatal infectious mononucleosis (im), to determine whether the cellular infiltrate had the immunophenotype and expressed epstein-barr virus (ebv)-encoded proteins characteristic of either ebv-immortalized lymphoblastoid cell lines (lcl) or ebv-carrying burkitt lymphoma (bl) cells. the results of these studies revealed that in 13 cases the proliferating b cells ... | 1990 | 2174416 |
| three cases of eb virus-associated uveitis. | we report our experience with three cases of epstein-barr virus (ebv)-associated uveitis. these patients had the following common clinical features: 1) a common cold-like prodrome, 2) bilateral involvement, 3) severe acute anterior uveitis with fibrinous exudate, 4) granulomatous anterior uveitis in the chronic stage, 5) little vitreous inflammation, 6) marked hyperemia and edema of the disc, 7) sunset glow fundus developed very early, 8) a good response to steroids but recurrence was common, 9) ... | 1990 | 2174420 |
| evidence for coiled-coil dimer formation by an epstein-barr virus transactivator that lacks a heptad repeat of leucine residues. | two regions of the epstein-barr virus (ebv) bzlf1 gene product, zebra, share sequence homology with c-fos, one of which corresponds to the dna binding domain of c-fos. zebra does not, however, contain the heptad repeat of leucines present in the dimerization domains of leucine zipper proteins. here it is shown that zebra binds its recognition sites as a homodimer and that the region adjacent to the basic dna binding domain is essential for dimerization. this region contains a 4-3 repeat of predo ... | 1990 | 2174563 |
| increased cell division as a cause of human cancer. | carcinogenesis research is increasingly focused on chemicals that are not genotoxic and yet, at high doses, can induce cancer, apparently by increasing cell proliferation. we hypothesize that increased cell division per se stimulated by external or internal factors is also associated with the development of many human cancers. although this hypothesis is well substantiated in the experimental literature, it has not been generalized as an important mechanism for carcinogenesis in human population ... | 1990 | 2174724 |
| j chain deficiency in human igm monoclonal antibodies produced by epstein-barr virus-transformed b lymphocytes. | six human igm monoclonal antibodies against pseudomonas aeruginosa were purified and characterized. on agarose-acrylamide sodium dodecyl sulfate (sds) gels run under nonreducing conditions, igm monoclonal antibodies showed variable amounts of a slower migrating form of igm in addition to the one co-migrating with plasma igm. protein blotting with anti-j chain antibody showed that the slower migrating form did not contain j chain. analysis of one of the monoclonal antibodies by sucrose gradient c ... | 1990 | 2174785 |
| detection of epstein-barr virus dna sequences in nasopharyngeal carcinoma cells by enzymatic dna amplification. | the presence of epstein-barr virus (ebv) dna sequences was examined by the polymerase chain reaction in 50 nasopharyngeal carcinoma (npc) biopsy specimens and in two primary epithelial tumor cell cultures derived from patients with npc. the detection limit was a single ebv genome equivalent by agarose gel electrophoresis followed by southern blot analysis of the amplified products. ebv dna sequences were detected in all 41 undifferentiated npc cell specimens, in 2 of 4 moderately differentiated ... | 1990 | 2174898 |
| comparative evaluation of two test methods (enzyme immunoassay and latex fixation) for the detection of heterophil antibodies in infectious mononucleosis. | the present study evaluated the ventrescreen mono enzyme immunoassay (ventrex laboratories) and monolatex (wampole laboratories) for detection of infectious mononucleosis-specific heterophil antibodies by comparing test results on sera of 247 symptomatic patients. the purpose of this study was to compare not only the two different test systems but also the two different methods they represent for accuracy in detecting infectious mononucleosis-specific heterophil antibodies. discrepancies in the ... | 1990 | 2174901 |
| [transforming genes of epstein-barr virus]. | | 1990 | 2174991 |
| [fatal acute encephalitis in primary epstein-barr virus infection]. | a 6 year-old girl presented an acute onset of mononucleosis-like syndrome which progressed at day 19 to encephalitis, coma and death at day 27. epstein-barr virus (ebv) serology suggested that she suffered from a primary ebv infection. therapeutic attempt with acyclovir did not improve the fatal course of the disease. | 1990 | 2175170 |
| pathogenesis of malignant lymphomas in intravenous drug-abuser, hiv-infected patients. | to study the pathogenesis of malignant lymphomas (ml) in intravenous drug-abuser hiv-infected patients, we analyzed 19 cases of reactive lymphadenopathy (las) and 10 cases of ml. clonality and differences in characteristics of these lymphoproliferative disorders were investigated by immunohistochemical and ig and tcr gene rearrangement analyses. rearrangements at the c-myc locus and presence of hiv and ebv viral genomes were also investigated. four out of the five non-hodgkin's lymphomas (nhl) a ... | 1990 | 2175249 |
| [monoclonal anti-d antibodies: obtaining and use]. | the principles of obtaining monoclonal antibodies against d-antigen of the rhesus system are overviewed. the lines secreting anti-d antibodies are characterized. the strategies for selecting the anti-d producers secreting the antibodies are substantiated, which are useful for developing rhesus-typing diagnostics and preparations to prevent rh-sensitization. | 1990 | 2176019 |
| oral hairy leukoplakia: a histopathologic study of 32 cases. | oral hairy leukoplakia (hl) was studied in 32 patients infected with the human immunodeficiency virus (hiv). features most often found were parakeratosis of variable thickness, acanthosis, little or absent submucosal chronic inflammatory cell infiltrate, association with candidiasis in 71.8%, and intranuclear herpetic type inclusions in keratinocytes, associated with ballooned or ground-glass cytoplasm. the last feature was found in all biopsies and was most striking at the top of the stratum sp ... | 1990 | 2176438 |
| molecular characterization of a cdna clone encoding the epstein-barr virus (ebv) dnase. | rna from idurd-treated p3hr1 cells was used for the construction of a cdna library and screened with b95-8 ebv dna bamhi fragment b and g probes. one clone, bg9, containing a 1.7 kb cdna insert was further studied. complete dna sequence analysis revealed that bg9 encompassed the b95-8 ebv dna sequences from nucleotide 120,747 to nucleotide 122,412 and corresponded to the bglf5 open reading frame of the ebv dnase gene. comparison of the sequences of bg9 with that of published b95-8 ebv dna indica ... | 1990 | 2176660 |
| oxidative burst in human epstein-barr virus-transformed b-cell lines triggered by immobilized specific antigen. | normal human b lymphocytes and epstein-barr virus-transformed b-cell lines can produce reactive oxygen species such as superoxide if treated with phorbol myristate acetate (pma) or with the surface immunoglobulin cross-linking agents protein a and anti-immunoglobulin. here, we investigated under which conditions specific antigen, the natural ligand of surface immunoglobulin, can stimulate an oxidative burst in monoclonal epstein-barr virus-transformed b-cell lines producing antibodies of known s ... | 1990 | 2176740 |
| immune deficiency as a risk factor in epstein-barr virus-induced malignant diseases. | epstein-barr virus (ebv) is a ubiquitous dna virus that normally infects silently, establishing lifelong latency. substantial empirical observations support the view that immunodeficiency is permissive in ebv-induced lymphoproliferative diseases (lpd). primary immune deficient patients such as those with x-linked lymphoproliferative disease and individuals with acquired immune deficiency secondary to immunosuppressive drugs for organ transplantation or individuals infected with human immunodefic ... | 1990 | 2176975 |
| multiple small bowel perforations in leukemia secondary to epstein-barr virus lymphoma with survival: a case report. | this is the case report of a 4-year-old white boy who was diagnosed as having acute lymphoblastic leukemia (all) in november 1985. while in remission and on maintenance chemotherapy, he developed a primary epstein-barr virus (ebv) respiratory infection in october 1986. on october 27, 1986 a plain abdominal radiograph taken for abdominal distention showed free air. at celiotomy, multiple nodules were noted to stud the small bowel. central necrosis of these nodules with perforations were present i ... | 1990 | 2177105 |
| role of atl-derived factor (adf) in the normal and abnormal cellular activation: involvement of dithiol related reduction. | htlv-i transformed t cells not only express a large number of interleukin-2 receptors (il-2r/p55(tac], but also produce an il-2r/tac inducer named atl-derived factor (adf). we have cloned the adf cdna and found that adf production in human lymphocytes can be enhanced by cellular activators such as mitogens or phorbol esters. recombinant adf produced by e. coli was shown to have growth-promoting activity in combination with interleukin-2 or suboptimal mitogenic stimuli on several lymphoid cells i ... | 1990 | 2177148 |
| c3d-mediated negative and positive signals on the proliferation of human b cells separated from blood. | soluble c3d applied to human blood-derived b lymphocytes inhibited anti-mu, t cell-produced growth factor, and ebv-induced dna synthesis in serum-free culture. c3d added to the b cell cultures 1 and 2 days after the stimulus, still exerted inhibition, though with gradually diminishing efficiency. c3d, fixed on zymosan or attached to the culture wells, induced [3h]thymidine incorporation of the b cells in serum-free medium. the concentration of c3d used to coat the wells was critical, with optima ... | 1990 | 2177450 |
| non-tumorigenicity of bloom's syndrome lymphoblastoid cell lines in immunodeficient mice. | | 1990 | 2177613 |
| ebv utilizes a unique activation pathway for the transformation of human b cells. | ebv growth-transforms primate b lymphocytes and directly causes mono/multiclonal b cell lymphomas in vulnerable hosts. in this report we demonstrate that the degree of b cell transformability is not quantitatively determined at the level of either the saturable, transformation-prerequisite virus receptors or of the actual viral cell entry process. instead, post-receptor binding events [na+/h+ exchange, ca2+ flux, tyrosine phosphorylation of two proteins (55-60/130-140 kd)] were identified as cri ... | 1990 | 2177646 |
| diagnosis of epstein-barr virus infection in hairy leukoplakia by using nucleic acid hybridization and noninvasive techniques. | the presence of epstein-barr virus (ebv) dna in the epithelial cells of oral hairy leukoplakia is the confirming criterion in the diagnosis of this lesion, which occurs mainly in persons infected by the human immunodeficiency virus. because hairy leukoplakia often presages the development of the acquired immune deficiency syndrome, it is important that suspicious lesions be accurately diagnosed. commonly, biopsy tissue is removed for detection of ebv dna by in situ hybridization, but biopsy is c ... | 1990 | 2177752 |
| medical eponyms updated: 3. burkitt's lymphoma. | | 1990 | 2177998 |
| isolation of human herpesvirus-6 from clinical specimens using human fibroblast cultures. | the isolation and characterization of human herpesvirus-6 (hhv-6) has been hindered by the lack of cell lines useful for its rapid propagation. recently, we have reported that the mrc-5 cell line (human diploid lung fibroblasts) was susceptible for hhv-6 infection. in this study, we report on the isolation of hhv-6 from the peripheral blood or buffy coat of three chronic fatigue syndrome patients, one post-liver transplant patient, and one severe chronic active epstein-barr virus syndrome patien ... | 1990 | 2178187 |
| toxoplasmic chorioretinitis and hepatic granulomas. | a 71-yr-old male presented with a 2-month history of fever, malaise, and weight loss. physical exam revealed chorioretinitis. laboratory studies were notable for elevated levels of alkaline phosphatase, gamma-glutamyl transpeptidase, aspartate transaminase, and alanine transaminase. immunoglobulin g antibody to toxoplasma gondii was positive to a dilution of 1:4096, whereas serologic studies for hepatitis a virus, hepatitis b virus, cytomegalovirus, epstein-barr virus, human immunodeficiency vir ... | 1990 | 2220741 |
| viral oncogenesis and the immune system. | oncogenic transformation of normal cells and the establishment of transformed cells to form malignant tumors is a complex, multistep process influenced by viruses in multiple ways. the relationship between viruses and the immune system manifests itself, in part, through various roles of viruses in transformation of host cells, including cells of the immune system. a large number of viruses participate in oncogenic transformation of cells in many animal species. candidates for oncogenic transform ... | 1990 | 2224909 |
| aftr: a fifth human b-cell-specific surface antigen. | we report the identification and characterization of a fifth b-cell-specific surface antigen (aftr) detected by three murine monoclonal antibodies. aftr is present on all malignant human b-cell lines tested except those of plasma cells and on malignant b cells from 49/49 patients, including those with pre-b-cell acute lymphoblastic leukemia. similarly, aftr is found on all cd19+ b cells from normal bone marrow, peripheral blood, and tonsil, although it is only weakly expressed on epstein-barr vi ... | 1990 | 2341301 |
| [establishment of a novel cell line derived from nasopharyngeal carcinoma]. | a novel epithelial cell line (designated as hne-1), derived from nasopharyngeal carcinoma (npc), was established and has passed more than 100 generations over one year. the npc biopsy specimen was taken from a 27 year old man with poorly differentiated squamous cell carcinoma of the nasopharynx. the cultured cells showed polygonal shape and grew into multilayers under the inverted microscope. electron microscopy showed that hne-1 cells were characterized by bi-directional differentiation with so ... | 1990 | 2364868 |
| fc receptor gene translocation in a t(1;19) pre-b all cell line. | we have recently mapped the human fcgr2 gene to chromosome 1 bands q23-q24. in situ hybridization of fcgr2 cdna with a cell line containing a t(1:19)(q23;p13) derived from a patient with pre-b all has allowed a more accurate localization of this gene to chromosome 1 band q23. furthermore, this study indicated a splitting of the fcgr2 gene or gene cluster by the t(1;19). however, southern analysis showed no genetic rearrangement when compared with a karyotypically normal epstein-barr virus (ebv)- ... | 1990 | 2370080 |
| immunostimulation by cytomegalovirus (cmv): helper t cell-dependent activation of immunoglobulin production in vitro by lymphocytes from cmv-immune donors. | cytomegalovirus (cmv) is the cause of a number of different diseases ranging from self-limited benign infections in healthy adults to life threatening illnesses among immunocompromised hosts and newborns. suppression of cell-mediated immunity is often found in cases of acute cmv infection, and in addition, the virus may also be a potent stimulant of lymphoid cells in vivo. we studied cellular proliferation and immunoglobulin (ig) production induced by cmv to determine its effect on human lymphoc ... | 1985 | 2409150 |
| a shared antigenic determinant between human b lymphocytes and carcinomas (blca). | we have raised a murine igm monoclonal antibody (ma6) against immunoprecipitate obtained by reacting the serum of an npc patient with extract of the burkitt lymphoma cell line, raji. it reacts against an antigen (blca) which is broadly represented on human b lymphoid tissues and cell lines but is different from the functional b-cell markers such as surface immunoglobulins, ia products, fc and complement receptors. blca was found to occur on lymphoid cell lines representing all stages of b-cell d ... | 1985 | 2411675 |
| monoclonal human anti-dna antibodies from eb virus-transformed lymphocytes of systemic lupus erythematosus (sle) patients. | sixteen monoclonal human anti-dna antibodies were obtained from epstein-barr virus-transformed lymphoblastoid cells of patients with systemic lupus erythematosus (sle) and were studied in terms of antigenic specificity. all of the antibodies showed polyspecificity to polynucleotides. among them, some antibodies had a specificity to single-stranded (ss) dna. especially, o-8 antibodies showed a preference for polynucleotides with pyrimidine bases. the binding specificity of the antibody was also s ... | 1985 | 2413066 |
| cdna of the immunoglobulin kappa chain of an epstein-barr virus-transformed human lymphoid cell line: partial sequence determination and bacterial expression. | we report the isolation, nucleotide sequence determination, and bacterial expression of a partial cdna for the immunoglobulin kappa chain from the epstein-barr virus-transformed human lymphoid cell line gm131. the cdna, cloned in pbr322 by use of oligo(dg) x oligo(dc) tails, yields two pst i fragments of 250 and 600 base pairs (bp). various restriction enzyme fragments of the cdna were subcloned in the vectors m13 mp10 and m13 mp11 for sequence analysis. as a result of instability of the 250-bp ... | 1985 | 2413453 |
| two monoclonal antibodies defining unique antigenic determinants on human b-lymphoma cells. | the non-hodgkin's lymphomas are a clinically, morphologically, and immunologically heterogeneous group of diseases. why lymphoma cells are unresponsive to normal regulatory growth controls and how they differ from normal lymphocytes are not well understood. in order to address these questions we have raised monoclonal antibodies to neoplastic b-cells. two of these, lm-26 and lm-155, show a high degree of specificity for b-cell lymphomas. when tested by fluorescence activated cell sorter analysis ... | 1985 | 2415242 |
| production of monoclonal antibodies against prostatic acid phosphatase by in vitro immunization of human spleen cells. | monoclonal antibodies against human prostatic acid phosphatase (papase) were produced by immunization of human primary spleen cell cultures. dissociated spleen cells were cultured for 5-8 days in the presence of 100 ng/ml of papase and pokeweed mitogen (1:5000). following immunization, b cells were isolated and infected with epstein-barr virus (ebv). two weeks after ebv-transformation, cells were fused with either mouse myeloma cells (sp2/oag14) or human/mouse heteromyeloma cells (shm-d33). hybr ... | 1985 | 2415632 |
| clonal analysis of hla-dpw1 (sb1) associated allodeterminants: recognition of novel epitopes and evidence for quantitative variation in class ii antigen expression. | alloreactive human t-lymphocyte clones were derived from the sb1 hla-dpw1)-specific, primed lymphocyte typing cell line sb1a used in the ninth international histocompatibility workshop. the clones from two separate subclonings were analyzed for their proliferative patterns in panel experiments with cells from unrelated individuals and in family segregation analyses. while only one clone gave a perfect correlation with the dpw1 specificity, the maturity of clones recognized multiple specificities ... | 1986 | 2419289 |
| isoprinosine abolishes the blocking factor-mediated inhibition of lymphocyte responses to epstein-barr virus antigens and phytohemagglutinin. | acute infectious mononucleosis (im) is accompanied by measurable abnormalities of immune function, including a transient immunosuppression. the sera of patients with acute im contain an igg blocking factor which binds to t-lymphocytes and decreases their responses to antigens and mitogens. the experiments reported herein indicate that isoprinosine, an immunopotentiating agent, can reverse this inhibition of t cells by im-associated igg blocking factor. isoprinosine may be a useful tool in unders ... | 1986 | 2420730 |
| interferon and 2-5a synthetase in patients with immune diseases. | | 1985 | 2421319 |
| epstein-barr virus-transformed lymphocytes from patients with bullous diseases produce autoantibodies to cytokeratins. | human cell lines obtained after epstein-barr virus transformation of lymphocytes from seven patients with bullous diseases (bullous pemphigoid, pemphigus) and five controls were screened for the production of autoantibodies against skin antigens. in five out of seven patients, the culture supernatants tested by indirect immunofluorescence on frozen sections of normal human skin and rabbit lip showed the production of autoantibodies with different specificities: basal epidermal cells, whole epide ... | 1986 | 2421952 |
| indirect alkaline phosphatase immunoenzymatic staining for the detection of antibodies to epstein-barr virus-induced virus capsid antigens and early antigens. | an indirect alkaline phosphatase immunoenzymatic staining technique was developed for the detection of antibodies against epstein-barr virus-induced virus capsid antigens and early antigens in cell smears. the presence of antibodies against epstein-barr virus-induced virus capsid antigens and early antigens was revealed by a dark blue staining of cells expressing the antigens. the alkaline phosphatase assay gave a permanent record of the reaction that could be visualized under an ordinary light ... | 1986 | 2422201 |
| an influenza a virus-specific and hla-drw8-restricted t cell clone cross-reacting with a transcomplementation product of the hla-dr2 and dr4 haplotypes. | the clone ta10 is a t3+ t4+ t8- proliferative and cytolytic human t cell clone. this clone has been shown to be specific for the hemagglutinin of influenza a texas virus and restricted by an hla class ii molecule associated with the drw8-dw8.1 phenotype. here we show that ta10 and all of its subclones can also react with eight hla-drw8 negative, epstein-barr virus (ebv)-transformed cell lines or phytohemagglutinin blasts in the absence of influenza antigens. all of these cell lines are hla-dr2/d ... | 1986 | 2422266 |
| characterization of the restricted component of epstein-barr virus early antigens as a cytoplasmic filamentous protein. | four monoclonal antibodies produced against the restricted component of the epstein-barr virus (ebv) early antigen (ea-r) precipitated a polypeptide with an approximate molecular weight of 85,000. three of these antibodies prepared against the native 85,000-molecular-weight protein (85k protein) reacted by immunofluorescence with acetone-fixed smears but not methanol-fixed smears of ebv-producing cells activated with tumor-promoting agent and sodium butyrate. the fourth monoclonal antibody which ... | 1986 | 2422401 |
| establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. | using a serum-free defined medium, we have established a human cell line, nci-h929, from a malignant effusion occurring in a patient with igak myeloma. the cultured cells have the morphologic, ultrastructural, biochemical, immunologic, and cytochemical features of plasma cells. the cells have rearranged alpha and kappa genes and synthesize and secrete high amounts of igak (greater than 80 micrograms/10(6) cells per 24 hours). the cells express surface immunoglobulin (alpha and kappa), the plasma ... | 1986 | 2423157 |
| antiviral therapy. new drugs and their uses. | | 1986 | 2425347 |
| functional analysis of the hla-a2/aw68 recombinant molecule hla-aw69: epstein-barr virus specific cytotoxic t cells restricted through aw69 can utilise restricting determinants on aw68 but not on a2. | epstein-barr virus-specific cytotoxic t cell lines, expanded in interleukin 2-conditioned medium, have been generated in vitro from virus-immune individuals bearing either the common hla-a2 antigen (a2.1), a variant a2 antigen (a2.2) or a novel antigen aw69 which is a recombinant molecule having identity with hla-aw68 in the alpha 1 domain and with hla-a2 in the alpha 2 and alpha 3 domains. virus-specific cytotoxic t cells restricted through a2.1 or a2.2 only recognised targets bearing the ident ... | 1986 | 2425456 |
| expression of the epstein-barr virus nuclear protein 2 in rodent cells. | a 3.0-kilobase-pair epstein-barr virus (ebv) dna segment necessary for lymphocyte immortalization encodes at least part of a nuclear protein (ebna2) which is characteristically expressed in latently infected, immortalized cells. a 1.5-kilobase open reading frame within this dna segment has now been inserted into a murine leukemia virus (mulv)-derived expression vector (pzip-neo-sv(x)1) which provides for transcription of heterologous dna but not for translational start. transfection of the recom ... | 1986 | 2426468 |
| natural killer susceptibility of human cells may be regulated by genes in the hla region on chromosome 6. | natural killer (nk) cells exist in each individual in the absence of any intentional immunization. they are able to kill a wide range of targets from tumoral as well as from normal origin. however, their exact physiologic role is not clearly understood. in this study we report results about a human epstein-barr virus-transformed b-cell line from which variants perturbed in the expression of hla molecules have been derived. our results indicate that in these cell lines an inverse relationship exi ... | 1986 | 2426704 |
| polymyositis and molecular mimicry, a mechanism of autoimmunity. | the amino acid sequences of escherichia coli histidyl-trna synthetase and alanyl-trna synthetase, two proteins recently identified as autoantigens in polymyositis, were compared by a computer alignment procedure with those of the 3600 proteins tabulated in the national biomedical research foundation protein sequence database. both proteins contain sequences long enough to function as epitopes that match sequences on viral and muscle proteins. the homology thus revealed not only lends strong supp ... | 1986 | 2427902 |
| monoclonal antibodies to hla-dp, dq, and dr determinants: functional effects on the activation and proliferation of normal and ebv-transformed b cells. | to investigate the function of hla class ii molecules in b-cell activation, we generated three new anti-hla class ii monoclonal antibodies with differing specificities for dp, dq, and dr determinants. these were tested for their ability to inhibit various b- and t-lymphocyte responses. each of these antibodies (nb-29, dh-84, and dh-224) immunoprecipitates a heterodimer of approximately 35,000 and 28,000 mol wt from 125i-surface-labeled b-lymphoma cells, as shown by sds-page. nb-29 (igg1) detects ... | 1986 | 2428652 |
| the glycine-alanine repeating region is the major epitope of the epstein-barr nuclear antigen-1 (ebna-1). | the epstein-barr nuclear antigen-1 (ebna-1) is a protein containing a large glycine-alanine repeat that has been shown to be antigenic. antibodies to ebna-1 can be detected by means of immunoblotting. preincubation of antisera with purified ebna-1 protein inhibits the binding of igg antibodies in this system, indicating that those epitopes detected by immunoblots are also accessible on the native molecule. a number of synthetic peptides the sequences of which were derived from the glycine-alanin ... | 1987 | 2432130 |
| treatment of virus-associated tumors with interferon. | | 1986 | 2434902 |