| clinical observations and virological study of aseptic meningitis in the kaohsiung area. | during 1988, an endemic outbreak of aseptic meningitis was noted in the kaohsiung area. throughout the year, a total of 89 cases were identified by cerebrospinal fluid (csf) examination at the pediatric department of kaohsiung medical college. the peak incidence was from june to october. scattered cases still occurred during november and december. the male to female ratio was 1.7:1 and the age distribution ranged from 1 month to 15 years old. two peaks of age distribution were observed; one in i ... | 1990 | 1981774 |
| early diagnosis of enteroviral meningitis by a solid-phase reverse immunosorbent test and virus isolation. | 45 cases of aseptic meningitis/meningoencephalitis were studied with regard to enteroviral etiology by virus isolation and solid-phase reverse immunosorbent test (sprist), a cross-reacting test for enterovirus igm. an etiological diagnosis was reached in 37/45 (82%) patients. etiological diagnoses other than enteroviruses were found in 8 patients: borrelia burgdorferi in 4, varicella-zoster virus in 2, herpes simplex virus in 1 and mumps virus in 1 patient. enteroviruses (echovirus 6, 21 and 30) ... | 1990 | 2175448 |
| characterization of an echovirus type 30 variant isolated from patients with aseptic meningitis. | an outbreak of echovirus type 30 infection associated with aseptic meningitis occurred among newborn babies in a hospital neonatal room at fukui city in 1983. the isolated virus was identified as an antigenic variant of echovirus type 30 by cross-neutralization tests with antisera against the prototype bastianni strain and the present isolate. western blot analysis demonstrated that the antigenic determinants responsible for virus neutralization, some of which were type specific and others strai ... | 1986 | 2425228 |
| waterhouse-friderichsen syndrome caused by haemophilus influenzae type b in an immunocompetent young adult. | a previously healthy 19-year-old woman had a febrile illness with hypotension, progressive cyanosis, and an evolving petechial rash. despite aggressive therapy in the face of shock and disseminated intravascular coagulation, the patient suffered a cardiac arrest and could not be resuscitated. haemophilus influenzae type b was cultured from the blood and echovirus 30 from the cerebrospinal fluid post mortem. fulminant h influenzae type b infection in an immunocompetent adult is rare but should be ... | 1989 | 2595428 |
| seroepidemiological studies of echovirus type 30 infection. | | 1986 | 3796325 |
| cryptosporidiosis outbreak in a day-care center. | an outbreak of diarrhea occurred in a day-care center in san marcos, tex, in august 1984. at the time of this study, the center was caring for 81 children aged 2 months through 5 years. a single stool specimen was collected from each of 50 children (62%). cryptosporidium oocysts and giardia lamblia cysts were each identified in stool specimens from 18 children. three children had both parasites present in the same stool specimen. echovirus 30 was identified in 19 of 25 stools cultured. although ... | 1985 | 4041130 |
| antibodies to echovirus type 30 in the canadian population. | | 1968 | 4176074 |
| echovirus type 30 infection: clinical and virological observations on an epidemic in western australia. | | 1973 | 4748566 |
| an epidemic of echovirus type 30 meningitis in an arctic community. | | 1974 | 4825602 |
| human diploid cell cutures in diagnosis of echovirus 30 meningitis. | | 1970 | 4986327 |
| characteristics of an echovirus type 30 strain isolated during an epidemic of aseptic meningitis in new york in 1968. | | 1971 | 4994458 |
| u.s. cases in 1968: epidemiology of echovirus 30 aseptic meningitis. | | 1972 | 5071313 |
| echovirus type 30 meningitis and related febrile illness: epidemiologic study of an outbreak in an eskimo community. | | 1970 | 5458218 |
| the seattle virus watch program. i. infection and illness experience of virus watch families during a communitywide epidemic of echovirus type 30 aseptic meningitis. | | 1970 | 5465459 |
| poliovirus and echovirus survival in tetrahymena pyriformis culture in vivo. | axenic cultures of tetrahymena pyriformis, strain i mt iv, grown in a defined medium at room temperature, were used to study interactions of these protozoa with vaccination strain l sc 2ab of poliovirus type 1, vaccination strain p 712 of poliovirus type 2 and with type 30 echovirus, strain 480/78. t. pyriformis cultures in media containing 10(3.0) tcd50/1 ml of type poliovirus, 10(3.0) tcd50/1 ml of type 2 poliovirus or 10(2.5) tcd50/1 ml echovirus 30 and in virus-free medium did not differ one ... | 1984 | 6088623 |
| elimination of some enteroviruses in the excrements of experimentally infected rats (rattus norvegicus) and gulls (larus ridibundus). | young rats of both sexes, weight 150-170 g, the first laboratory progeny of captured wild parent pairs, were used throughout this experiment. rats in two experimental groups comprising a total of 34 animals were infected orally with type 2 poliovirus vaccine strain given in each group at doses of 500, 5000 or 50,000 tcd50. in the first experiment, the presence of poliovirus in rat excrements was detectable irregularly till day 13, in the second experiment till day 2 after infection. small quanti ... | 1984 | 6092458 |
| survival of polioviruses and echoviruses in acanthamoeba castellanii cultivated in vitro. | cultures of acanthamoeba castellanii, kept at room temperature in sterile bacto-casitone (difco) medium, were artificially infected with vaccination poliovirus strains type 1 and type 3 and with echovirus type 4 and echovirus type 30. no remarkable virus cumulation was observed on the surface or inside amoeba cells during the observation period of 21 days. amoeba-adsorbed viruses were impossible to remove by repeated washings. virus neutralization with specific antisera showed that enteroviruses ... | 1981 | 6265550 |
| [infections of the central nervous system caused by enterovirus: 223 cases seen at a pediatric hospital between 1973 and 1981]. | between 1973 and 1981, 223 patients were seen at hôpital sainte-justine in montreal for enteroviral infection of the nervous system. in 161 the cause was documented by isolation of an enterovirus from the cerebrospinal fluid (csf). the viruses most frequently isolated were echovirus 11 (36 isolates), echovirus 30 (24), coxsackievirus b2 (23), coxsackievirus b3 (19), echovirus 6 (18), coxsackievirus b5 (16), coxsackievirus a9 (15), echovirus 9 (13), echovirus 7 (12) and coxsackievirus b1 (11). as ... | 1982 | 6291736 |
| [isolation of 503 strains of virus in meningeal syndromes. virologic and epidemiologic study]. | an epidemiological and virological study on meningitis in alsace during the 13-year period 1968-1981 is reported. 503 viruses isolated were associated with the diagnosis of meningitis. the echovirus type 30 accounted for 48 p. 100 of the cases, the mumps virus of 12 p. 100. the remarkable high incidence of echo 30 virus-associated cases is related to three outbreaks which occurred in alsace during the summers of 1968, 1975 and 1980. children below 12 years of age are the most sensitive populatio ... | 1983 | 6351218 |
| echovirus type 30: 1980. | | 1981 | 6783259 |
| molecular epidemiology of enterovirus outbreaks in canada during 1991-1992: identification of echovirus 30 and coxsackievirus b1 strains by amplicon sequencing. | the relatedness of enteroviral isolates associated with two recent outbreaks in canada was assessed using direct sequencing of amplicons derived from a large portion of the 5' nontranslated region (ntr) of the viral genome. the amplicons of 60 echovirus 30 isolates originating from seven different provinces in 1991 were found to share 99% or greater sequence identity. recent coxsackievirus b1 isolates characterised in the same manner were identical to each other. when the 5' ntr sequence of thes ... | 1994 | 7534817 |
| outbreak of aseptic meningitis in taipei in spring 1993. | an outbreak of aseptic meningitis in the taipei area occurred from january to august 1993. a total of 114 cases were identified by cerebrospinal fluid (csf) examination at national taiwan university hospital. most of the cases were found from february to may 1993. the peak of age distribution was 3 to 11 years. the male-to-female ratio was 2.1 to 1. most patients had fever, headache, and vomiting and nausea. on initial csf examination, protein concentrations > 400 mg/l were noted in 38 of 93 csf ... | 1995 | 7613228 |
| sequence comparison of echovirus type 30 isolates to other enteroviruses in the 5'noncoding region. | the genetic relationship between echovirus type 30 (e30) isolates were characterised by means of amplification of a part of the 5'noncoding region by polymerase chain reaction and direct sequencing. later, the e30 sequences were compared with other enteroviruses. homology between recent clinical e30 isolates from different years exceeds 98% at the nucleotide level. comparing the e30 sequence with other enteroviruses, homology varied between 68% (coxsackievirus a24) and 93% (coxsackievirus b3). e ... | 1995 | 7636503 |
| clinical characteristics, management strategies, and cost implications of a statewide outbreak of enterovirus meningitis. | a statewide outbreak of enterovirus meningitis occurred in rhode island in the summer of 1991. a comprehensive chart review was conducted for determining the demographic and clinical characteristics of the epidemic and for assessing the interinstitutional variation in treatment strategies. four hundred eight cases were reported, and enteroviruses were recovered in 61 (68%) of 90 cases in which viral isolation was attempted. of six isolates that were serotyped, all were echovirus 30. analysis of ... | 1995 | 7795097 |
| quantitative detection of neutralizing antibodies against polioviruses and non-polio enteroviruses (npev) using an automated microneutralization assay: a seroepidemiologic survey. | a seroepidemiological survey for the assessment of immunity against polioviruses and non-polio enteroviruses (npev) seroprevalence was performed using a recently established automated microneutralization assay. the seroprevalences of neutralizing antibodies against coxsackie viruses b1-b6, and a9, echoviruses 6, 9, 11, and 30, and polioviruses 1, 2, and 3 were investigated in serum samples collected over a period of 30 months (january 1990 to june 1992). overall, npev seroprevalence ranged from ... | 1994 | 8061416 |
| echovirus 30 infection and aseptic meningitis in parents of children attending a child care center. | in july 1992, 13 parents with children attending a child care center (ccc) developed aseptic meningitis (am) due to echovirus 30 (e30). to determine the extent of illness and risk factors for transmission, survey and blood specimens were collected from ccc families and teachers and from adult and pediatric controls. infection was defined as the presence of anti-e30 igm antibodies. ccc parents (60%, 67/111) and children (75%, 79/105) had significantly higher infection rates than did teachers (14% ... | 1994 | 8169408 |
| echovirus type 30 infection associated with aseptic meningitis in nassau county, new york, usa. | an outbreak of echovirus type 30, primarily affecting infant under 1 year old, was documented in nassau county, n.y., usa. twenty-four of the 40 infected patients were definitively diagnosed with aseptic meningitis. a positive correlation was found between the proportion of polymorphonuclear leukocytes and the overall cerebrospinal fluid white cell count. | 1993 | 8225911 |
| [epidemic of aseptic meningitis with echovirus type 30 in gifu prefecture]. | during july to december in 1991, an epidemic of aseptic meningitis occurred in gifu prefecture in children. epidemiological, virological and serological investigations were performed. the results are as follows: 1) cases involved ranged from 0 to 13 years of age, and 75.9% of them were in the 3 to 7 year of age group. 2) three types of enteroviruses were isolated from 83 of 133 cases (62.4%), and the echovirus type 30 was recovered from 71 cases (85.5%). 3) in 1991 with the cross neutralizing te ... | 1993 | 8270799 |
| an unseasonable winter outbreak of echovirus type 30 meningitis. | during the winter of late october, 1990 to march, 1991, echovirus type 30 meningitis affected 10 children aged 3-9 years of age in mikuni, japan. five patients presented with constipation, four had diarrhoea, while five complained of severe abdominal pain, in addition to typical symptoms and signs compatible with aseptic meningitis. echovirus type 30 was isolated from the csf of nine, from the faeces of 10, and from the throats of eight children. all showed a significant antibody response to ech ... | 1995 | 8586842 |
| intratypic genome variability of echovirus type 30 in part of the vp4/vp2 coding region. | the genetic relationship of 33 echovirus type 30 (e30) isolates associated with three different outbreaks of meningitis in norway and one outbreak in usa was assessed using direct sequencing of amplicons derived from a region covering part of the capsid proteins vp4 and vp2. the e30 sequences were compared to each other, and to other enteroviruses. less sequence variation was observed between the isolates from a single outbreak (2-3%) than between groups of isolates from different outbreaks (4-9 ... | 1996 | 8678835 |
| [meningitis by enterovirus in pediatrics. clinical characteristics and virologic diagnosis]. | with the aim of knowing the etiology of aseptic meningitis (pleocytosis in lcr with negative bacterial culture and positive or negative virus culture or without pleocytosis with viral culture in positive lcr), 48 children attending the emergency department of our hospital from june to december, 1995 were prospectively studied. pharyngeal and rectal swab, lcr for bacteria and virus and blood cultures were carried out. the samples were inoculated in fibroblasts mrc-5, rd and bgm. the cytopathic ef ... | 1998 | 9542303 |
| diagnosis and epidemiology of echovirus 22 infections. | to find out whether the appearance and pathogenicity of infections caused by echovirus 22 (ev22)--which has considerably different molecular characteristics from those of typical enteroviruses--exhibit extraordinary features, novel diagnostic approaches were applied. staining of inoculated cell cultures with specific antibodies was followed by immunoperoxidase detection. of fecal specimens from 140 children under 6 years of age, four were positive by the immunoperoxidase method. the nucleotide i ... | 1998 | 9675466 |
| detection by pcr of enteroviruses in cerebrospinal fluid during a summer outbreak of aseptic meningitis in switzerland. | enteroviruses (ev) are among the most common causes of aseptic meningitis. standard diagnostic techniques are often too slow and lack sensitivity to be of clinical relevance. ev rna can be detected within 5 h by a commercially available reverse transcription-pcr (rt-pcr) test kit. cerebrospinal fluid (csf) samples from 68 patients presenting with aseptic meningitis during a summer outbreak in switzerland were examined in parallel with cell culture and commercial rt-pcr. rt-pcr was positive in al ... | 1998 | 9705364 |
| [outbreak of aseptic meningitis in iwamizawa, 1997, caused by echovirus 30]. | to investigate the clinical character of an outbreak of aseptic meningitis in iwamizawa 1997 caused by echovirus 30, and to investigate the spreading of the outbreak, we analyzed clinical character of 75 hospitalized patients in our hospital, and mapped the patients' distribution in iwamizawa city each week. we detected in our hospital an epidemic outbreak of acute enteroviral meningitis caused by echovirus type 30 in iwamizawa, from september to december, 1997. regarding the patients, there was ... | 1998 | 9745226 |
| aseptic meningitis due to echovirus 30, japan, 1997-1998. | | 1998 | 9763936 |
| rapid detection of enteroviral rna in cerebrospinal fluid (csf) from patients with aseptic meningitis by reverse transcription-nested polymerase chain reaction. | the aim of this study was to compare conventional enterovirus isolation with rapid detection of enteroviral rna by a reverse transcription-nested polymerase chain reaction (rt-npcr) method amplifying the 5' nontranslated region of the enteroviral genome in specimens from patients with aseptic meningitis. reference enterovirus strains and clinical enterovirus isolates were analyzed to evaluate assay sensitivity and specificity. all known enteroviral serotypes tested, but one (echovirus type 22), ... | 1998 | 9812315 |
| outbreak of echovirus 30 meningitis in southern saskatchewan. | | 1998 | 9864878 |
| aseptic meningitis due to echovirus 30, japan, 1997-1998. | | 1998 | 9864879 |
| epidemics of aseptic meningitis due to enteroviruses following national immunization days in bahrain. | we report two successive epidemics of aseptic meningitis due to enteroviruses (ev) observed after national immunization days against polio. meningitis due to echovirus 30 occurred from july 1995 to the end of january 1996, mostly among children aged 0-12 years (95.1% of cases), and meningitis due to echovirus 4 occurred from may 1996 to the end of september 1996 in the same age group. there were 286 and 169 cases, respectively. specimens from several representative cases were sent to the who col ... | 1998 | 9924570 |
| community-wide outbreak of enteroviral illness caused by echovirus 30: a cross-sectional survey and a case-control study. | in june, 1997, 21 children from a single community in germany were hospitalized with aseptic meningitis. an epidemiologic investigation was conducted to determine the extent of the outbreak and risk factors for illness. | 1999 | 10048680 |
| [an epidemic of aseptic meningitis caused by echoviruses in fukuoka prefecture during april 1997 to august 1998]. | an epidemic of aseptic meningitis in fukuoka prefecture during april 1997 to august 1998 was studied to determine the serotype of viruses isolated in fukuoka prefecture. in fukuoka prefecture, bimodal peaks were seen in july and december 1997. monthly changes of reported aseptic meningitis patients and period of virus isolation revealed that epidemics of the earlier part in 1997 was caused by echovirus 9 (e 9) and the latter part due to echovirus 30 (e 30). e 9 was isolated mainly in chikugo are ... | 1999 | 10213990 |
| [the importance of polymerase chain reaction in the diagnosis of enterovirus infections of the central nervous system in children. clinico-epidemiologic characteristics]. | to compare the diagnosis utility of a reverse transcription-polymerase chain reaction (rt-pcr) for detection of enteroviral rna in cerebrospinal fluid (csf) in comparison to viral culture for central nervous system infections in pediatric population and to know the clinic epidemiological characteristics of this infection. | 1999 | 10396087 |
| a study of enterovirus isolations in glasgow from 1977 to 1997. | the number and range of enteroviruses isolated in the regional virus laboratory, glasgow during 1977-1997 was determined. over this period, 3,039 enterovirus isolations were reported. the echoviruses represented 67% of isolations with echovirus 4 (due to an outbreak in 1990), echovirus 30 and echovirus 11 being the most frequently isolated types. the pattern of prevalence of non-polio enteroviruses had changed from the previous 20-year period with echovirus types isolated more often (77% vs. 55. ... | 1999 | 10447428 |
| an outbreak due to echovirus type 30 in a neonatal unit in france in 1997: usefulness of pcr diagnosis. | between february and august 1997, 53 patients with enterovirus meningitis were hospitalized in clermont-ferrand, france. all but one were children. echovirus type 30 was involved in 70% of cases with identified serotype. the outbreak ceased on august 8. two months later, a neonate was admitted to the neonatal unit with an echovirus type 30 meningitis thought to be acquired at delivery. twenty days later a nosocomial outbreak of echovirus type 30 involving five neonates occurred. two of them pres ... | 1999 | 10462641 |
| an echovirus 30 outbreak with a high meningitis attack rate among children and household members at four day-care centers. | echovirus 30 (e 30) outbreaks in defined cohorts have rarely been reported. in june 1996, an outbreak of e 30 occurred in four day-care centers (dccs) in neighboring villages in germany. a retrospective cohort study of dcc children, employees and household members was done to determine the extent of the outbreak and risk factors for illness. forty-two percent (39/92) of dcc children, 13% (30/228) of their household members, 5% (1/19) of employees and 2% (1/49) of household members of employees w ... | 1999 | 10543356 |
| phylogenic analysis of echovirus type 30 isolated from a large epidemic of aseptic meningitis in japan during 1997-1998. | during 1997 to 1998, a nationwide epidemic of aseptic meningitis occurred in japan. more than 4,500 isolates from patients with aseptic meningitis were identified as echovirus type 30. to investigate the character of these isolates, we examined the nucleotide sequences of thirty-seven geographical representatives and compared them with 50 strains isolated during the past 20 years. the phylogenic analysis used partial sequences from either the vp1 or vp4-vp2 region of the viral capsid. this analy ... | 1999 | 10592896 |
| [clinical study of an outbreak of aseptic meningitis due to echovirus type 30 in munakata city in 1997-1998]. | from october, 1997 through july, 1998, an outbreak of aseptic meningitis due to echovirus type 30 occurred in the northern part of kyushu area in japan. in this outbreak, clinical and virologic observations were carried out on 157 in-patients with aseptic meningitis at our hospital. the age of the patients ranged from 1 year and 9 months to 57-year old. one hundred and twenty out of 157 cases were the children under 15 years of age, and in this age group, male/female ratio was 2:1. the largest p ... | 2000 | 10783577 |
| echovirus 30 outbreak of aseptic meningitis in turkey. | | 2000 | 10819358 |
| duration of echovirus 30 excretion in stools from patients with aseptic meningitis. | | 2000 | 10957714 |
| genetic variability within the vp1 coding region of echovirus type 30 isolates. | genetic relationships of the prototype bastianni strain of 1958 and of 13 echovirus type 30 (ecv30) isolates associated with meningitis cases in germany during a period from 1966 to 1997 were investigated using direct sequencing of amplicons derived from a part of the capsid protein vp1 gene. sequences were aligned both with each other and with known sequences of other type 30 echovirus strains. phylogenetic analysis indicated that isolates investigated in this work fell into at least three gene ... | 2000 | 10963349 |
| enteroviruses in spain: virological and epidemiological studies over 10 years (1988-97). | a total of 15,662 clinical samples were analysed for enterovirus (ev) isolation in cell cultures during a 10-year period (1988-97). furthermore, 210 isolates of ev obtained in primary laboratories within spain from patients with meningitis were characterized. the total number of ev typed was 758, including 727 non-polio ev and 31 sabin-like (sl) polioviruses. twenty-eight ev serotypes were represented. echoviruses comprised 90% (653/727) of fully typed non-polio ev. the four most prevalent serot ... | 2000 | 10982074 |
| nosocomial transmission of echovirus 30: molecular evidence by phylogenetic analysis of the vp1 encoding sequence. | we investigated six cases of enterovirus infection in a neonatal unit. the index patient, a 5-day-old boy, was admitted with aseptic meningitis due to echovirus 30 (e30). secondary infections with e30 occurred in five babies. comparison of the complete vp1 sequences showed that the isolates recovered from the index patient and his mother were closely related to those recovered from the five babies with secondary infections, demonstrating a nosocomial transmission of the virus. in the phylogeneti ... | 2000 | 10921945 |
| molecular epidemiology of echovirus 30 in europe: succession of dominant sublineages within a single major genotype. | the genetic relationships between 131 echovirus type 30 (e-30) field isolates were studied using phylogenetic analysis of three genomic intervals: vp4/vp2 (420 nt), the entire vp1 and vp1/2a (150 nt). the strains had been isolated between 1975-1998, in different european countries, and in israel and japan. the maximum genetic variation was 15.7% in the vp4/vp2 region, 21.3% across the vp1/2a junction and 16.7% in the vp1-gene. the clustering patterns were very similar in all three regions. two d ... | 2001 | 11338388 |
| enterovirus infections in germany: comparative evaluation of different laboratory diagnostic methods. | the diagnosis of an enterovirus infection may be achieved through direct virus detection from fecal or cerebrospinal fluid (csf) samples by virus isolation or pcr. serologically, a significant rise in antibody titer may be detected and different enteroviral types can be differentiated using the neutralization assay. | 2001 | 11440383 |
| molecular characterization of human enteroviruses in clinical samples: comparison between vp2, vp1, and rna polymerase regions using rt nested pcr assays and direct sequencing of products. | three nested rt-pcr assays were developed to permit sensitive typing of enteroviruses directly from clinical samples. these assays amplified short fragments from different genomic regions codifying for three proteins: vp2, vp1, and rna polymerase. given that enteroviruses have a high rate of degeneration within target codons among serotypes, the primers used consisted of mixed base and deoxyinosine residues. these techniques detected at 0.03-0.003 tcid50 of prototype poliovirus 1 and echovirus 3 ... | 2001 | 11505456 |
| outbreak of echovirus 30 meningitis in switzerland. | this study includes 80 patients (38 children and 42 adults) who contracted aseptic meningitis in the summer of 1996 in fribourg, switzerland. virological studies revealed an enteroviral infection in 65 out of 70 (93%) investigated patients. in 47 out of the 53 cases (89%) where a precise virus could be identified, the causative agent was an echovirus 30. more than 50 patients lived in an area within a 5-km radius. the patients presented with the classic symptoms and signs of aseptic meningitis. ... | 1999 | 10680981 |
| molecular typing of echovirus serotype 4 isolates. | we report on clinical samples stuttgart/97, berlin/99 and jasi/99 associated with aseptic meningitis. all three samples contained echovirus 4 (e4) but stuttgart/97 was simultaneous infected with echovirus 30 (e30). the genetic relationship of the e4 strains was assessed using rt-pcr and direct sequencing of amplicons derived from the genomic region encoding the capsid protein vp1. the sequences have been compared with each other and with sequences of further e4 strains obtained from genbank. the ... | 2001 | 11597753 |
| human surveillance for west nile virus infection in ontario in 2000. | the first reports of west nile virus (wnv) infection in the united states in 1999 prompted ontario to establish a surveillance protocol to monitor for the possible spread of the virus into the province. surveillance components included evaluation of dead birds, sentinel chickens, mosquito pools and human disease. we report the results of human surveillance in 2000. | 2002 | 11800244 |
| genomic variations in echovirus 30 persistent isolates recovered from a chronically infected immunodeficient child and comparison with the reference strain. | seven sequential isolates of echovirus type 30 (ev30) were recovered over 22 months from a child with severe combined immune deficiency syndrome. the nucleotide sequences of the 5' halves of the genomes (4,400 nucleotides) of the first (s1) and last (s7) isolates were determined and compared with that of the ev30 bastianni reference strain, also determined in this study. in genome regions p1 and p2, 101 variations were identified between the two isolates. synonymous differences far outnumbered n ... | 2000 | 10655344 |
| echovirus 30 meningitis: a new cause for cerebral venous thrombosis? | | 2002 | 11972424 |
| [lymphocytic meningitis in spain: a possible epidemic situation in 2000]. | in order to clarify the unexpected increase in 2000 of lymphocyticmeningitis analyzed in the centro nacional de microbiología,instituto de salud carlos iii, madrid, here we present their microbiological features. | 2002 | 12042133 |
| benefits of management strategy adjustments during an outbreak of enterovirus meningitis in adults. | enteroviruses (evs) are the most common identifiable cause of the aseptic meningitis syndrome. widespread seasonal outbreaks of ev meningitis result in a high financial cost to the community, in part because of the difficulty of discriminating between viral and bacterial meningitis. during a nationwide outbreak of ev meningitis due to echovirus 30 in france we tested the hypothesis that a management strategy including early testing of cerebrospinal fluid (csf) by means of ev pcr in all adult pat ... | 2002 | 12069020 |
| functional impairment and killing of human beta cells by enteroviruses: the capacity is shared by a wide range of serotypes, but the extent is a characteristic of individual virus strains. | direct infection of beta cells could explain the diabetogenic effect of enteroviruses. primary adult human beta cells are susceptible to coxsackievirus infections, which could result in impaired beta-cell function or cell death (coxsackieviruses b3, b4, b5) or both, or no apparent immediate adverse effects (coxsackievirus a9). we extended these studies to additional enterovirus serotypes including several echoviruses, some of which have been associated clinically with the development of type i ( ... | 2002 | 12107750 |
| molecular epidemiology and genetic diversity of echovirus type 30 (e30): genotypes correlate with temporal dynamics of e30 isolation. | echovirus type 30 (e30) (genus, enterovirus; family, picornaviridae) has caused large outbreaks of aseptic meningitis in many regions of the world in the last 40 years. u.s. enterovirus surveillance data for the period 1961 to 1998 indicated that the annual proportion of e30 isolations relative to total enterovirus isolations has fluctuated widely, from a low of 0% in 1966 to a high of 42% in 1998. peaks of e30 isolations occurred in the years 1968 to 1969, 1981 to 1984, 1990 to 1993, and 1997 t ... | 1999 | 10565909 |
| a hospital outbreak of aseptic meningitis due to echovirus type 30 in antalya, turkey. | we analyzed clinical and laboratory findings of 23 hospitalized patients with aseptic meningitis in the department of pediatrics, akdeniz university hospital. the patients presented with the classic symptoms and signs of aseptic meningitis. protein levels of the cerebrospinal fluid (csf) samples ranged from 18 to 99 mg/dl, with a mean of 36.5 +/- 4.9 mg/dl. the mean ratio of csf glucose compared to blood samples was 0.73. echovirus type 30 was identified in csf and/or stool samples of 19 patient ... | 2002 | 12405436 |
| laboratory diagnosis and genetic analysis of an echovirus 30-associated outbreak of aseptic meningitis in taiwan in 2001. | a large outbreak of aseptic meningitis occurred from april to november 2001 in taiwan. of the 1,130 enterovirus-infected patients, echovirus 30 (e30) infection was diagnosed in 188 (16.6%), with the patients having various clinical manifestations including aseptic meningitis (73.9%), young infant fever (6.9%), respiratory symptoms or herpangina (13.3%), or others (5.9%). the majority of the e30-infected patients were between 3 and 10 years old. of the 264 e30 strains identified, 94.3, 71, and 67 ... | 2002 | 12454133 |
| molecular identification and typing of enteroviruses isolated from clinical specimens. | enterovirus characterization and typing require an integrated technological approach, using both immunological and molecular methods. the seventy-nine enteroviruses included in this study were isolated from cell cultures and classified as enteroviruses on the basis of an indirect immunofluorescence assay (ifa) against common enterovirus antigens and a neutralization test based on the lim benyesh-melnick (lbm) pool. the final identification was carried out using a number of different molecular ap ... | 2002 | 12454151 |
| molecular and biological analysis of echovirus 9 strain isolated from a diabetic child. | the full-length infectious cdna clone was constructed and sequenced from the strain dm of echovirus 9, which was recently isolated from a 6-week-old child at the clinical onset of type 1 diabetes. parallel with the isolate dm, the full-length infectious cdna clone of the prototype strain echovirus 9 barty (barty-inf), was constructed and sequenced. genetic relationships of the sequenced echo 9 viruses to the other members of the human enterovirus type b species were studied by phylogenetic analy ... | 2003 | 12601761 |
| three echovirus serotypes responsible for outbreak of aseptic meningitis in rhône-alpes region, france. | during the year 2000 in the rhône-alpes region of france, 559 cases of aseptic meningitis due to enterovirus infection were recorded (approximate incidence, 10 cases per 100,000 population compared with a mean of 0.3 cases during endemic years in this region; p<0.001). cerebrospinal fluid samples were collected from all 559 patients and processed for enterovirus rna detection using a reverse transcriptase polymerase chain reaction assay only. in addition to the cerebrospinal fluid samples, 40 st ... | 2003 | 12649719 |
| severe echovirus 30 infection in twin neonates. | although enteroviruses can cause overwhelming and fatal systemic infections in neonates, such severe neonatal infections remain uncommon and rarely involve both of twin neonates at the same time. we report the cases of twin neonates who developed fever initially, and then progressed to disseminated systemic disease with marked thrombocytopenia, coagulopathy, and hepatic failure. one of the neonates died and the other survived. both neonates were treated with intravenous immunoglobulin and matern ... | 2003 | 12684616 |
| evolution of the genome of human enterovirus b: incongruence between phylogenies of the vp1 and 3cd regions indicates frequent recombination within the species. | enteroviruses show a high degree of sequence variation both between and within serotypes due to the lack of proofreading of the viral rna-dependent rna polymerase. in addition, recombination is known to occur not only within but also between different serotypes. we have previously shown that capsid coding sequences of coxsackievirus b4 (cvb4) cluster in several coexisting genotypes (intergenotypic nucleotide difference of 12 % or more) whereas a single lineage of echovirus 30 (ev30) has been pre ... | 2003 | 12692288 |
| viral meningitis in child care center staff and parents: an outbreak of echovirus 30 infections. | a report of five cases of viral meningitis among adults with children enrolled in a child care center prompted an investigation of risk factors for viral transmission from children to adult household members. | 1999 | 10476994 |
| outbreaks of aseptic meningitis associated with echoviruses 9 and 30 and preliminary surveillance reports on enterovirus activity--united states, 2003. | aseptic or viral meningitis is the most common type of meningitis and is associated with an estimated 26,000--42,000 hospitalizations each year in the united states. enteroviruses are the most common cause of aseptic meningitis. echovirus 9 (e9) and echovirus 30 (e30) have been associated frequently with outbreaks of aseptic meningitis. during march 2003, several state public health departments noted increased reports of aseptic meningitis and, as of august 7, seven states (arizona, california, ... | 2003 | 12917581 |
| an outbreak of aseptic meningitis due to echovirus type 30 in two cities of turkey. | an outbreak of aseptic meningitis due to echovirus 30 occurred in ankara and antalya in turkey, during june to september 1999, with 176 cases fitting the clinical case definition. cases were ascertained from attendees of the three hospitals in ankara and one in antalya. medical files were reviewed and evaluated retrospectively. clinical presentation and laboratory findings were typical of viral meningitis. cerebrospinal fluid and/or stool samples of 86 patients were cultured for enteroviruses. e ... | 2003 | 12974559 |
| echovirus type 30 and meningitis. | | 1964 | 14129812 |
| assessment of an enterovirus sewage surveillance system by comparison of clinical isolates with sewage isolates from milwaukee, wisconsin, collected august 1994 to december 2002. | the quantity and serotypes of enteroviruses (evs) in the influent of a local sewage treatment plant were compared to local clinical ev cases to determine if testing of sewage is adequate for an ev surveillance system. the study was carried out from august 1994 to december 2002. monthly influent specimens were processed by organic flocculation, and dilutions of concentrate were inoculated onto a number of different cell types for virus isolation. evs were detected in 88 of 100 monthly influent sa ... | 2003 | 14660364 |
| an approach based on rflp assay to investigate outbreaks of enteroviral meningitis. | enteroviruses (ev) circulate worldwide and are a major cause for annual epidemics of meningitis in humans. during the last two decades, echovirus type 30 (e-30) has revealed to be one of the most prevalent enteroviruses at the origin of epidemics of ev meningitis. | 2004 | 14675871 |
| laboratory diagnosis of enteroviral infections of the central nervous system by using a nested rt-polymerase chain reaction (pcr) assay. | enteroviruses are the most common pathogens identified in infants hospitalized for suspected aseptic meningitis. rapid detection of enterovirus infection is essential in taking the decision for treatment with antiviral agents and applying infection control measures in hospitalized pediatric patients. the purpose of this study was to compare the results of conventional virus isolation with those of enteroviral rna detection by reverse transcription (rt)-pcr method in identical specimens from case ... | 2003 | 14711475 |
| [pcr sscp study of an echovirus 30 meningitis outbreak]. | between april and october 1997, 21 children of 4 days to 13 years old were admitted to the pedatric unit of aulnay sous bois's hospital for viral meningitidis. the number of white blood cells in the cerebrospinal fluid (csf) was between 1 and 612 cells/mm3, with, on an average, 56% of segmented cells, 34% lymphocytes and 34% monocytes. proteins and glucose of csf were standard. one csf was normal. viral meningitidis was confirmed by viral culture of csf onto mrc5. enterovirus were identified by ... | 1999 | 10418035 |
| analysis of the serotype and genotype correlation of vp1 and the 5' noncoding region in an epidemiological survey of the human enterovirus b species. | the sequence identity of the enterovirus vp1 gene has been shown to correlate with the serotype concept. enterovirus molecular typing methods are therefore often based on sequencing of the vp1 genomic region and monophyletic clustering of vp1 sequences of a homologous serotype. for epidemiological surveillance, 342 enterovirus samples obtained from patients with aseptic meningitis in belgium from 1999 to 2002 were first diagnosed as being enterovirus positive by amplification of the 5' noncoding ... | 2004 | 15004039 |
| [detection of nonpoliomyelitic enteroviruses in central nervous system infections in poland in 1995-2000; viral isolation data]. | the aim of this study was evaluation of enterovirus diagnosis based on isolation of the virus in cell culture from subjects with aseptic neuroinfection conducted in six provincial sanitary-epidemiological stations in 1995-2000 years. available data from six sanitary-epidemiological stations indicated that 1429 isolation of enteroviruses from fecal samples of those patients were performed. the most frequently isolated enterovirus serotype was echovirus type 30: 138/203 isolated enteroviruses, but ... | 2003 | 15029839 |
| [clinical observations in the enterovirus cerebrospinal meningitis outbreak in the płotsk region in the fall of 1995]. | clinical and epidemiological observations in a small outbreak of enterovirus cerebrospinal meningitis were presented. the epidemic consisted of 154 cases who were hospitalized in the observation and infectious disease departments of the provincial hospital in płock. the outbreak started on september 7, 1995 and lasted 2 months up to november 19, 1995. the initial cases were school children. there were 92 males and 62 females, 64% of patients (92 cases) were inhabitants of urban areas. 114 cases ... | 1998 | 10321092 |
| identification of enteroviruses by indirect immunofluorescence using monoclonal antibodies. | serum neutralization (nt) is used most often to type enterovirus isolates, but it is labor-intensive, expensive, and supplies of reference antisera for nt are limited. alternative methods of enterovirus typing are needed. | 1995 | 15566790 |
| [viral meningoencephalitis caused by enterovirus in cuba from 1990-1995]. | the results of a study on enterovirus as causal agents of viral meningoencephalitis from 1990 to 1995 are described. from the 1458 samples of patients studied, 586 were from feces, 108 from cerebrospinal fluid and 1095 were paired sera. the samples for viral isolation were inoculated in two different cellular systems. a total of 225 samples (32.42%) were found to be positive; the highest number of isolations (n = 217) was found in feces and in diploid human lung fibroblast cells (phue-1). antibo ... | 1997 | 9472139 |
| [outbreak of meningitis caused by echovirus type 30]. | in the cold months of 1994-1995, we detected in our hospital an epidemic outbreak of acute enteroviral meningitis caused by echovirus type 30 (echo 30). until now, these outbreaks of viral epidemics had not been detected in spain. | 1997 | 9173841 |
| an outbreak of viral meningitis associated with a public swimming pond. | from july to october 2001, 215 cases of aseptic meningitis occurred among the inhabitants of the german city of kassel and neighbouring counties. a matched case-control study identified bathing in a public, nature-like pond during the beginning of the outbreak as a risk factor for disease [matched odds ratio (mor) 44.8, 95% confidence interval (ci) 3.9-515.6]. among bathers, patients with meningitis spent more time in the water (mor 18.8, 95% ci 2.0-174.1) and swallowed water more frequently (mo ... | 2005 | 15816154 |
| echovirus 30, jiangsu province, china. | an outbreak of aseptic meningitis occurred in the northern area of jiangsu province in china from january to july in 2003. a total of 1,681 cases were involved in this outbreak, and 99% of patients were <15 years of age. to identify the etiologic agent, 66 cerebrospinal fluid specimens were tested by cell culture. eighteen showed an enteroviruslike cytopathic effect on mrc-5 human fetal diploid lung cells. an enterovirus primer-mediated reverse transcriptase-polymerase chain reaction, a standard ... | 2005 | 15829194 |
| [serotype distribution of enteroviruses isolated from paediatric cases prediagnosed as aseptic meningitis between 2001-2004 period]. | enteroviruses have major clinical and public health importance and are one of the leading causes of aseptic meningitis. there are many diseases with similar clinical symptoms and cerebrospinal fluid (csf) findings of aseptic meningitis, thus virus isolation and identification is crucial for definitive diagnosis. virological diagnosis is nonetheless important to distinguish between induced meningitis and other treatable causes of disease with a similar clinical picture. a total of 249 samples obt ... | 2005 | 15900836 |
| the clinical evaluation of an outbreak of aseptic meningitis in children. | aseptic meningitis is a common disease in children; the present report describes the clinical and laboratory studies of 62 children involved in an outbreak of aseptic meningitis. | 1996 | 8634928 |
| analysis of cytokine levels in cerebrospinal fluid in mumps meningitis: comparison with echovirus type 30 meningitis. | it is unclear whether or not the csf cytokine profiles in viral meningitis differ with the kind of causative virus. | 2005 | 15927848 |
| [sequence analysis of echovirus type 30 isolated from an aseptic meningitis outbreak in northern jiangsu province in 2003]. | to determine the partial sequence of virus strains causing an aseptic meningitis outbreak in northern part of jiangsu province in 2003 and to compare them with the same serotype strains isolated in other countries to better understand its genetic characteristics and hereditary trend of development. | 2005 | 15941539 |
| replication of respiratory viruses, particularly influenza virus, rhinovirus, and coronavirus in huh7 hepatocarcinoma cell line. | detection of viral antigens and isolation methods has long been used for the diagnosis of respiratory virus infections. the objective was to determine the ability of huh7 cells to support the replication of prototype and wild strains of respiratory viruses. the cell culture-adapted strains of influenza viruses a and b, parainfluenza viruses 1-4, respiratory syncytial viruses a and b, both strains of the human metapneumoviruses, numerous rhinoviruses, most of the adenoviruses, coronaviruses 229e ... | 2005 | 16121382 |
| [investigation of enterovirus in cases of aseptic meningitis syndrome of belém, pa]. | with the objective of isolating and identifying enteroviral serotypes, which are the most frequent etiologic agents of aseptic meningitis syndrome, cerebrospinal fluid samples were studied from patients referred to the health unit in the city of belém (state of pará, brazil), from march 2002 to march 2003. the samples were inoculated in rd and hep-2 cells line, and the positive were identified by neutralization test or indirect immunofluorescence. out of 249 samples, 33 (13.2%) were positive 57. ... | 2005 | 16172753 |
| an epidemic of encephalitis and meningoencephalitis in children caused by echovirus type 30 in shanghai. | an outbreak of viral central nervous system infection occurred from may to september, 1991 in shanghai, china. totally 376 cases were admitted into our hospital, 54% with encephalitis and 46% with meningoencephalitis. echovirus type 30 was isolated from csf and stools of the patients. only 3 of the patients were under 1 year of age. csf examination revealed the cell count from 0-1600 x 10(6)/l. interestingly, 9 had csf pmns over 90% and 13 had low sugar levels. ekg was randomly done in 20 patien ... | 1993 | 8033610 |
| a community outbreak of echovirus infection associated with an outdoor swimming pool. | forty-six people became ill with vomiting, diarrhoea and headache within days of an outdoor swimming pool opening for the summer season in a small seaside village. during the weekend of the outbreak, 185 tickets to the pool had been sold. it was found that 34 bathers were ill, and one subject had vomited into the pool. all other cases arose after this incident. the risk of infection was greatest among those who swallowed pool water (24/28 versus 10/17, p = 0.07). echovirus 30 was isolated from t ... | 1994 | 7946487 |
| epidemics of aseptic meningitis due to echovirus 30 in japan. a report of the national epidemiological surveillance of infectious agents in japan. | two rages of epidemic of aseptic meningitis (am) due to echovirus 30 (e30) in japan were analyzed with respect to two sources of information, am incidence and e30 isolation, both gathered through the national epidemiological surveillance of infectious diseases. the first e30 epidemic spread throughout japan in 1983 and ceased within the year. the second epidemic, starting in 1989, continued for the three successive years, and in the last year, 1991, the total e30 reports numbered 4,061, the larg ... | 1994 | 7715095 |
| transverse myelitis due to echovirus type 30. | | 2005 | 16421057 |
| an outbreak of aseptic meningitis due to echovirus 30 associated with attending school and swimming in pools. | to identify the risk factors of an outbreak of meningitis associated with echovirus 30-infection that occurred in rome, italy, in late 1997 among children from two different schools. | 2006 | 16458563 |
| enterovirus surveillance--united states, 2002-2004. | enteroviruses are common viruses associated with diverse clinical syndromes, ranging from minor febrile illness to severe, potentially fatal conditions (e.g., aseptic meningitis, encephalitis, paralysis, myocarditis, and neonatal enteroviral sepsis). a total of 68 enterovirus serotypes are recognized, including 65 nonpolio enteroviruses. individual serotypes have different temporal patterns of circulation and can be associated with different clinical manifestations. this report describes trends ... | 2006 | 16484979 |
| [laboratory network within the polio eradication initiative (1998-2003): six years of surveillance for acute flaccid paralysis in spain]. | worldwide poliomyelitis eradication was proposed in 1988 by the world health organization (who), based on reaching and maintaining high vaccination coverage and on implementing effective poliovirus infection surveillance systems. | 2006 | 16606558 |