| studies of an epstein-barr virus-induced dna polymerase. | | 1977 | 14436 |
| appearance and some unusual features of epstein-barr virus antibody production in infectious mononucleosis and other health disorders. | the indirect immunofluorescence (if) test and the complement-fixation reaction (cfr) were used in examination of over 1500 sera obtained from patients with infectious mononucleosis (im) and other health disorders. the evidence obtained supports a direct aetiological relationship between epstein-barr virus (ebv) and im and points on a relationship of ebv to some other lymphadenopathies and health disorders. the incidence of the igg type antibody against virus capsid antigen (eb-vca) and soluble a ... | 1977 | 15437 |
| differential expression of d and r components of epstein-barr virus early antigen after superinfection and after induction with 5-iododeoxyuridine. | three non-productive human lymphoblastoid cell lines, nc-37, raji and l-1, either superinfected with epstein-barr (eb) virus from p3hr-1 cells or treated with 5-iododexyuridine (iudr), were studied for the development of early antigen (ea) of eb virus. in all three superinfected cell lines, both the restricted (r) and diffuse (d) componets of ea were synthesized, with r-component formation preceding d-component synthesis. after iudr treatment both components of ea were only formed in raji cells, ... | 1977 | 18915 |
| opportunistic non-hodgkin's lymphoma in x-linked recessive immunodeficiency and lymphoproliferative syndromes. | | 1977 | 21454 |
| mosquito-virus relationships of american encephalitides. | | 1978 | 24407 |
| epstein-barr virus infection following bone-marrow transplantation. | a 12-year-old patient with acute lymphoblastic leukemia received a bone-marrow transplant (bmt) from a matched sibling donor. nine weeks prior to transplant the donor experienced epstein-barr virus (ebv)-induced infectious mononucleosis. the bone-marrow recipient was ebv-negative at the time of transplant; however, 4 weeks post transplant the recipient developed clinical symptoms of graft-verus-host disease (gvhd) coincident with serological evidence of acute ebv infection. in addition, a lympho ... | 1978 | 29839 |
| immunovirological examination of patients with lymphoblastic and prolymphocytic lymphosarcomas. | | 1978 | 30234 |
| epstein-barr virus infections in childhood. | igm and igg antibodies against epstein-barr virus (ebv) capsid antigen and antibodies against ebv nuclear antigen and heterophil antibodies were investigated in 115 paired sera of children with acute infections and in 100 sera of healthy controls of the same age and sex. ebv-specific igm antibodies could be recognized in 13.7% of the patients and in 7% of the controls. antibodies against ebv nuclear antigen were not detected in the igm-positive sera. | 1979 | 39436 |
| enhancement of epstein-barr virus antibody production in systemic lupus erythematosus patients. | two groups of 22 patients suffering from either systemic lupus erythematosus (sle) or infectious mononucleosis (im) were checked for epstein-barr virus capsid antigen antibody (eb-vca) production. the average significant antibody levels as well as the frequency of their occurrence were clearly higher in sle than in im patients. | 1979 | 39442 |
| letter: infectious mononucleosis, academic performance, and emotional stability. | | 1975 | 46553 |
| infectious mononucleosis: model for self-limiting lymphoproliferation. | evidence for the extensive lymphoproliferation which characterises infectious mononucleosis (i.m.) is summarised, and some of the mechanisms which are though to control lymphoproliferative activity in this disease are discussed. the main host responses which appear to limit lymphoproliferation in i.m. include the development of humoral antibodies against the epstein-barr (e.b.) virus-associated membrane antigen (present on the e.b. viral envelope and on e.b.-virus-infected cells) and cellular im ... | 1975 | 48066 |
| x-linked recessive progressive combined variable immunodeficiency (duncan's disease). | of 18 boys in duncan kindred, 6 died of a lymphoproliferative disease. they exhibited a subtle, progressive combined variable immunodeficiency disease characterised by benign or malignant proliferation of lymphocytes, histiocytosis, and alterations in concentrations of serum-immunoglobulins. infectious mononucleosis occurred during or preceding terminal events in at least 3 of the cousins. fever, pharyngitis, lymphadenomegaly, hepatosplenomegaly, atypical lymphocytosis, and a spectrum ranging fr ... | 1975 | 48119 |
| lymphoblastoid transformation and kinetics of appearance of viral nuclear antigen (ebna) in cord-blood lymphocytes infected by epstein-barr virus (ebv). | human cord-blood lymphocytes were infected with b95.8 epstein-barr virus (ebv) before and after separation into b- and t-cell populations. lymphoblastoid cells exhibiting b-cell characteristics appeared after 2 to 3 days of culture in the total population and in the separated b-cell subpopulation but not in the t-cell subpopulation. ebv nuclear antigen (ebna) was detected concurrently with the appearance of lymphoblastoid cells. the proportion of ebna-positive cells corresponded to that of lymph ... | 1975 | 49325 |
| tumor viruses: 1974. | | 1975 | 50896 |
| the epstein-barr virus and malignancy: molecular evidence. | | 1975 | 50897 |
| hodgkin's disease. | | 1975 | 51322 |
| mononucleosis-associated subacute sclerosing panencephalitis. | three cases of coincidental infectious mononucleosis (i.m.) and subacute sclerosing panencephalitis (s.s.p.e.) in children are reported. in two of the cases, i.m. preceded the appearance of s.s.p.e. symptoms. the other case of s.s.p.e. was associated with apparent mononucleosis encephalitis. all three patients died within 3 months of s.s.p.e. being diagnosed and had pathological changes characteristic of that disease and raised levels of measles antibody. two of the patients had apparent t-cell- ... | 1975 | 51346 |
| critical evaluation of b and t cell markers in man. | | 1975 | 53874 |
| ebv-determined nuclear antigen (ebna)-positive cells in the peripheral blood of infectious mononucleosis patients. | after removal of srbc rosette-forming t-cells from the peripheral blood, the residual, largely b-lymphocyte fraction of five infectious mononucleosis patients was found to contain 0.5-2% blast cells, positive for the ebv-determined nuclear antigen (ebna). there was a rough parallelism between the presence of large lymphoblasts in the hematological smear, ebns-positive large blasts in the b-cell fraction and the ability of the t-cell fraction to exert an ebv-specific lymphocytotoxicity on establi ... | 1976 | 55396 |
| pathogenesis and phenotypes of an x-linked recessive lymphoproliferative syndrome. | a new x-linked recessive lymphoproliferative syndrome has variable phenotypes: fatal infectious mononucleosis (i.m.), agammaglobulinaemia after i.m., american burkitt's lymphoma, histiocytic lymphoma, immunoblastic sarcoma of b cells, or plasmacytoma. an immunodeficiency to rubeola and the epstein-barr virus probably ensues from the mutant gene. the phenotypes (spectrum of b-cell disorders) have a common inheritance and the aetiology is similar. | 1976 | 62116 |
| inducibility of the epstein-barr virus (ebv) cycle and surface marker properties of ebv-negative lymphoma lines and their in vitro ebv-converted sublines. | two ebv-negative lymphoma lines of human b-cell origin, bjab and ramos, were compared with altogether six of their in vitro ebv-converted, ebna- and ebv-dna-carrying sublines (four of ramos and two of bjab derivation). all converted lines closely resembled the parental line with regard to karyotype and hl-a and b antigen typing. induction of ebv antigens (ea and vca) by p3hr-1 virus superinfection was either similar in the converted and the negative lines, or somewhat increased in certain conver ... | 1976 | 62724 |
| aetiology of nasopharyngeal carcinoma. | | 1976 | 63856 |
| detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anti-complement immunofluorescence, and use of a blocking assay to demonstrate their specificity. | skin fibroblasts exposed to cytosine arabinoside (ara c) were infected with either cytomegalovirus (cmv) or herpes simplex virus (hsv) type 1 and 2. herpesvirus-determined early antigens (hv-ea), detected by anti-complement immunofluorescence (acif), occurred primarily in the nucleic, and the specificity of these results was established by an acif blocking reaction using f(ab')2 fragments of human and hyperimmune reference sera. direct tests with selected sera and cross-blocking experiments betw ... | 1977 | 64461 |
| complex pathogenesis of cancer in transplant recipients. | | 1977 | 65239 |
| comparison of dna polymerase activities from an american burkitt's lymphoma cell line and ebv producer and nonproducer cells. | | 1975 | 65315 |
| comparative studies on ebv antigens by immunofluorescence and immunoperoxidase techniques. | three groups of ebv antigens, vca, ma and ea, were compared by the techniques of electron microscopic immunoperoxidase (ip) and immunofluorescence (if). p3hr-1 and ebv superinfected raji cells served as targets for labelled sera from patients with bl, npc and im or from healthy donors. 125i peroxidase-labelled antibodies were also prepared to determine, autoradiographically, the penetration of the complex into the cell system, and to monitor the incubation and washing procedures. the development ... | 1977 | 66208 |
| an immunofluorescence technique with counterstain on fixed cells for the detection of antibodies to human herpesviruses; antibody patterns in patients with hodgkin's disease and nasopharyngeal carcinoma. | an indirect immunofluorescence (if) test on fixed cells with evans' blue counterstain is described for all four human herpesviruses, i.e., herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2), varicella-zoster virus (vzv), cytomegalovirus (cmv) and epstein-barr virus (ebv). comparison with immunodiffusion (id) for hsv-2 and with id and complement fixation (cf) for vzv and cmv demonstrated the specificity and high sensitivity of the if test. also introduced is a modification of the anti-compl ... | 1976 | 67099 |
| is burkitt's lymphoma related to perinatal infection by epstein-barr virus? | burkitt's lymphoma (b.l.) is very restricted geographically, whereas the epstein-barr virus (e.b.v.), a very likely causal factor, exists all over the world. it is proposed that perinatal infection (either transplacental, or, more probably, neonatal) is an important risk factor for the development of b.l. this hypothesis arose when the epidemiological characteristics of e.b.v. infection were compared in different parts of the world and is supported by the preliminary results of a seroepidemiolog ... | 1977 | 64861 |
| subpopulations of human lymphoblastoid cell lines. correlation with the expression of surface receptors and content of epstein-barr virus genome. | forty lymphoblast cell lines derived from normal subjects, patients with infectious mononucleosis, leukemia, and burkitt's lymphoma have been studied for surface receptors including surface ig, complement receptors by the eac rosette and fluorescent (raji cell) techniques, and fc (aggregate) receptor by direct and indirect immunofluorescence. because of the b-cell tropism of the epstein-barr virus (ebv), an effort was made to correlate the expresion of various surface properties of lymphoblastoi ... | 1976 | 62390 |
| identification of a purified complement-fixing antigen as the epstein-barr-virus determined nuclear antigen (ebna) by its binding to metaphase chromosomes. | a soluble complement-fixing antigen carried by epstein-barr virus (ebv)-transformed human cells has been previously extracted from cell nuclei and purified by dna-cellulose chromatography [luka, j., siegert, w. & klein, g. (1977) j. virol., in press]. on addition of this antigen to methanol/acetic acid-fixed metaphase chrmosomes, followed by exposure to human sera containing antibodies against the ebv-determined nuclear antigen (ebna), brilliant positive staining was obtained by anti-complement ... | 1977 | 67603 |
| studies on epstein-barr virus-related antigens. i. indirect single radial immunodiffusion as a useful method for detection and assay of soluble antigen. | a useful method for the detection and assay of epstein-barr virus (ebv)-related soluble antigen has been developed by the application of the indirect single radial immunodiffusion technique which is frequently used for quantitative measurements of immunoglobulins and other soluble proteins. when the extracts of ebv-determined nuclear antigen (ebna)-positive non-producer cells (raji and nc-37) were applied to agar plates containing seropositive human serum, followed by overlay with anti-human igg ... | 1976 | 61945 |
| [virus etiology of lymphomas and leukemias in man]. | | 1976 | 61149 |
| unusual prevalence of antibodies to epstein-barr virus early antigen in ataxia telangiectasia. | | 1977 | 68263 |
| molecular probes for tumor viruses in human cancer. | | 1976 | 60297 |
| oncogenic viruses: implications for human diseases (part ii). | | 1976 | 60244 |
| surface markers on human b and t lymphocytes. viii. association between complement and epstein-barr virus receptors on human lymphoid cells. | an association has been found between the epstein-barr virus (ebv) and complement (c3d) receptors on human lymphoid cells. the evidence was four-fold: there was a correlation between the expression of these two receptors; inhibition experiments showed that the binding sites probably are close to each other in the cell membrane, although not identical; ebv and complement receptors have been found to co-cap in either order; and lymphoid cell lines lacking complement receptors could not be superinf ... | 1976 | 59946 |
| observations on the antigenic relationships between epstein-barr virus and herpesvirus saimiri. | the antigenic relationships between epstein-barr (eb) virus and herpesvirus saimiri (hvs) have been investigated in comparative immunofluorescence, microimmunodiffusion and serum neutralization tests. no similarity was detected between the structural antigens of the two herpesviruses but the results of microimmunodiffusion tests showed that they shared a non-structural, virus-determined antigen. the implications of this finding are discussed in relation to the importance of hvs and its monkey ly ... | 1977 | 70507 |
| somatic cell hybrids between human lymphoma lines. ii. spontaneous and induced patterns of the epstein-barr virus (ebv) cycle. | the regulation of spontaneous, iudr-induced and p3hr-1 virus-induced ea and vca production patterns was studied in two new somatic hybrids between human lymphoma lines. the hybrid 8a was derived from the crossing of the non-producer raji with the spontaneous producer daudi line. the second hybrid, 83, was produced by the fusion of raji with the ebv-genome-negative b-lymphoma line, bjab. the studies suggest the following ebv regulation patterns: (1) the spontaneous production of ea and vca appear ... | 1976 | 59708 |
| the absence of b-cell antigen b2 from leukaemia cells and lymphoblastoid cell lines. | 60 leukaemia patients with acute mylogenous leukaemia, chronic myelocytic leukaemia, acute lymphatic leukaemia, and chronic lymphocytic leukaemia were tested for five specificities of the new b-lymphocyte alloantigenic system. two specificities of the first b-cell locus were significantly lower in frequency than in b lymphocytes from 105 controls. b-cell group 1 was found in 18% of leukaemia patients vs. 48% of controls (p less than 0.025) and b-cell group 2 was found in 0% of leukaemia patients ... | 1976 | 59011 |
| multiple-sclerosis-associated agent. | | 1977 | 71613 |
| [virus and cancer]. | | 1977 | 71664 |
| immunological control of human lymphoma: discussion. | | 1976 | 56225 |
| lymphocyte transformation induced by autologous cells. iii. lymphoblast-induced lymphocyte to stimulation does not correlate with eb viral antigen expression or immunity. | human mitogen-induced and cell line b lymphoblasts stimulate the proliferation of allogeneic and autologous lymphocytes in culture. the role in thes reaction of eb viral determinants on the stimulating cells and immunity of the lymphocyte donor to the eb virus has been studied. the stimulatory capacity of cultured cell line lymphoblasts is not inhibited by incubating lymphoblasts with antisera to eb viral determinants. cultured cell line b lymphoblasts stimulate as much thymidine incorporation b ... | 1976 | 55437 |
| persistence of epstein-barr viral nuclear antigen (ebna) in cells entering the eb viral cycle. | it was shown by double immunofluorescence studies that epstein-barr viral nuclear antigen (ebna) was preserved in ebv-infected cells after they had entered the productive viral cycle, as signalled by the appearance of the early antigen (ea)complex. a nuclear component of the ea comples could be clearly distinguished from ebna with regard to antigenic specificity. | 1976 | 55398 |
| detection of a nuclear, ebna-type antigen in apparently ebna-negative herpesvirus papio (hvp)-transformed lymphoid lines by the acid-fixed nuclear binding technique. | in agreement with the findings of previous authors, we could not detect a virally determined nuclear antigen in herpesvirus papio (hvp)-transformed baboon lymphoid lines by anticomplementary staining in situ, as for ebna. however, by means of our recently developed acid-fixed nuclear binding technique an ebna-like antigen could be readily demonstrated, after extraction from both producer and non-producer lines. we propose to designate the antigen as hupna. it can be detected by a human anti-ebna ... | 1977 | 73523 |
| epstein barr virus and nasopharyngeal carcinoma. | | 1976 | 73653 |
| clinical and serological analysis of transfusion-associated hepatitis. | of 108 prospectively followed, multiply transfused, open-heart-surgery patients, 12 (11%) developed hepatitis. patients received only volunteer donor blood tested for hepatitis-b surface antigen (hbsag) prior to transfusion by counterelectrophoresis (c.e.p.). 4 of the 12 patients developed hepatitis-b-virus infection. subsequent testing of donor serums by solid-phase radioimmunoassay (r.i.a.) revealed that an r.i.a.-positive, c.e.p.-negative blood unit was transfused to 3 of the 4 type-b hepatit ... | 1975 | 53329 |
| epstein-barr virus-induced transformation of human leukocytes after cell fractionation. | the efficiency of transformation of human lymphocytes after infection with epstein-barr virus (ebv) was determined in fractionated and non-fractionated preparations derived from 16 human cord blood samples and two blood samples from adult donors. the transformation efficiency of macrophage-depleted leukocytes was consistently lower as compared to non-fractionated leukocytes. additional depletion of b-cells resulted in a further decrease. reduction of t-cells, however, did not influence significa ... | 1975 | 47842 |
| effects of 5-tungsto-2-antimoniate in oncogenic dna and rna virus-cell systems. | | 1977 | 74337 |
| serum iga antibodies to epstein-barr virus capsid antigen preceding symptoms of nasopharyngeal carcinoma. | | 1978 | 75457 |
| production of rabbit antibodies against the viral capsid antigen (vca) of the epstein-barr virus (ebv). | rabbits were immunized with nuclear or cytoplasmic extracts of the epstein-barr virus (ebv)-producing marmoset cell line b95-8. following extensive absorption with human ebv-negative cells (hep-2, ramos and bjab), sera were obtained that no longer reacted with cellular or serum proteins of human origin, but gave a single precipitin band with extracts of the human ebv-producing line, p3hr-1. immunofluorescence tests performed with appropriate parallel human serum controls showed that the rabbit s ... | 1978 | 75856 |
| blastogenic response and ebna induction in human lymphocytes by epstein-barr virus only requires b cells but not macrophages. | the postulated role of macrophages in the primary infection of human lymphocytes by epstein-barr virus (ebv) was examined. macrophage removal had no effect on the blastogenic response but slightly reduced the percentage of ebna-positive cells induced by the b 95-8 virus strain. both the rate of dna synthesis and the appearance of ebna-positive cells could rather be related to the number of b cells in the infected populations. macrophages are thus not required for the initiation of ebv-induced ly ... | 1977 | 45722 |
| epstein-barr virus infection in staff treating patients with nasopharyngeal carcinoma. | | 1978 | 76242 |
| abortive growth of human lymphocytes carrying a dormant epstein-barr viral genome. | when p3hr-1 lymphoblastoid cells expressing epstein-barr virus (ebv)-related antigens at very low frequency were cocultivated with human umbilical cord blood lymphocytes and the cell-free mixed culture fluid was applied to fresh cord lymphocytes, cells morphologically distinct from normal lymphocytes became evident after one to two weeks' exposure. the abnormal cells became abundant after one month and were easily identified by b-cell markers and a variety of morphologic abnormalities. such abno ... | 1978 | 45600 |
| epstein-barr-specific iga in nasopharyngeal cancer. | | 1978 | 78104 |
| observations on cell lines derived from a patient with hodgkin's disease. | permanent cell lines have been established from a spleen nodule and lymph node of a male hodgkin's disease (hd) patient whose father has the same disease. th in vitro growth pattern morphological and cytogenetic characteristics of these lines maintained continuously for over 2 years are described. the cultures contain a population of mixed cell types that grow in suspension. between 5 and 10% of the cells have surface immunoglobulins m and d. b-cell alloantigens are also detectable. while the cu ... | 1978 | 78764 |
| colonies of ebna-positive cells in soft agar from peripheral leukocytes of infectious mononucleosis patients. | epstein-barr virus (ebv)-associated nuclear antigen (ebna)-positive lymphoblastoid cells grew as colonies in soft agar after seeding of leukocytes from the peripheral blood of four patients with infectious mononucleosis serologically determined to be caused by ebv. in individual cases more colonies were obtained from blood specimens during the acute phase of the disease than during the convalescent phase. incorporation of human umbilical cord serum, which contained neutralizing antibody to ebv, ... | 1978 | 78908 |
| intracranial heterotransplantation of human hematopoietic cells in nude mice. | a panel of established cell lines and many primary cell specimens from lymphomas and leukemias as well as from normal lymphatic tissues were tested for tumorigenicity by intracranial heterotransplantation in nude mice. not only lymphoma and leukemia cell lines, but also lymphoblastoid cell lines, lacking markers of malignancy, were tumorigenic in the brains of nude mice. these findings indicate that tumorigenicity following intracranial heterotransplantation in nude mice cannot be used as proof ... | 1979 | 38211 |
| persistent epstein-barr virus infection in a child with hypergammaglobulinaemia and immunoblastic proliferation associated with a selective defect in immune interferon secretion. | a 5-year-old girl had a chronic disease characterised by fever, lymphoid hyperplasia, interstitial pneumonitis, thrombocytopenia, and polyclonal hypergammaglobulinaemia. evidence for severe, persistent epstein-barr virus (e.b.v.) infection was found: titres of antibody to e.b.v. viral capsid antigen (igm and igg) and early antigen were extremely high, cells containing e.b.v.-associated nuclear antigen (e.b.n.a.) were found in lymph nodes and blood, and spontaneous permanent lymphoblastoid cell l ... | 1978 | 79029 |
| epstein-barr virus and burkitt lymphoma. | | 1978 | 79925 |
| uv-inactivation of epstein-barr virus: differences in early antigen expression in two different non-productive cell lines and influence of caffeine. | two non-productive epstein-barr (eb) virus genome-carrying lymphoblastoid cell lines, namely raji and nc37, were used for studying the effect of uv irradiation on the ability of p3hr-1 eb virus to induce early antigen (ea) formation. in nc37 cells infected with uv-irradiated virus the formation of ea was delayed; thus the slope of inactivation curve based on the early (24 hr) reading was steeper than that based on the late (72 hr) reading. this was not observed in raji cells. caffeine did not in ... | 1978 | 30263 |
| late persistence of serum gamma-glutamyl transpeptidase activity after mononucleosis. report of 3 cases. | gamma-glutamyl transpeptidase (ggtp) is a sensitive but nonspecific index hepatobiliary disease. in infectious mononucleosis (im) or the mononucleosis-like disease attributable to cytomegalovirus (cytomegalovirus-induced im), ggtp reverted to normal later than aspartate aminotransferase and alkaline phosphatase. in three cases elevated serum ggtp activity persisted for up to 24 months -- raising the question of persistent 'post-im' hepatitis. such prolonged ggtp activity was unusual in other lat ... | 1977 | 15921 |
| papain solubilization of the epstein-barr virus-induced membrane antigen. | the epstein-barr virus (ebv)-induced membrane antigen (ma) was successfully solubilized from the membranes of viable ebv-infected raji cells by treatment with papain (5 to 6 u per 1 x 10(7) to 2 x 10(7) cells). the loss of ma from viable cells was monitored by membrane immunofluorescence and antibody-dependent cellular cytotoxicity. soluble ma was demonstrated in papain digests through inhibition of antibody-dependent cellular cytotoxicity and by inhibition of the binding of anti-ma antibodies t ... | 1978 | 81320 |
| epstein-barr antibodies in suspected nasopharyngeal carcinoma. | | 1978 | 82106 |
| epstein-barr virus d.n.a. in tumour tissue from native alaskan patients with nasopharyngeal carcinoma. | | 1978 | 82107 |
| studies on epstein-barr virus-related antigens. ii. biochemical properties of soluble antigen in raji burkitt lymphoma cells. | biochemical properties of epstein-barr virus (ebv)-related soluble antigen in non-producer raji burkitt lymphoblastoid cells, assayed by the indirect single radial immunodiffusion (isrd) test, were investigated. the soluble isrd antigen retained activity even after exposure to a temperature of 80 degrees c. the antigen was precipitated in 40% saturated ammonium sulfate and isrd activity was recovered from the precipitate when reconstituted into a solution. isoelectric focusing and crossed immuno ... | 1977 | 14897 |
| tumorigenicity of human hematopoietic cell lines in athymic nude mice. | human hematopoietic cell lines, which had been classified on the basis of studies on clonality, and morphological, chromosomal and functional parameters as lymphoblastoid cell lines (lcl) of presumed non-neoplastic origin, and lymphoma, myeloma and leukemia lines of proven malignant origin, were tested for tumorigenic potential on subcutaneous transplantation to nude mice and for capacity to grow in semi-solid medium in vitro. recently established lcl failed to grow both in nude mice and in agar ... | 1977 | 14896 |
| somatic cell hybrids between human lymphoma cell lines. v. idurd inducibility and p3hr-1 superinfectability of daudi/hela (dad) and daudi/p3hr-1 (dip-1) cell lines. | we have studied two types of somatic cell hybrid with regard to expression of the epstein-barr virus (ebv) cycle and its regulation. the first, dip-1, a hybrid formed between two human lymphoma ebv producers (daudi and p3hr-1), contained ebv dna, expressed the virus-determined nuclear antigen (ebna), andwas a producer of the ebv-associated antigens ea (early antigen) and vca (viral capsid antigen). the second, dad, a hybrid series of clones formed between daudi and a hela cell derivative (d98), ... | 1978 | 82545 |
| comparison between two antibody populations in the ebv system: anti-ma versus neutralizing antibody activity. | ebv-neutralizing antibody titers were determined in 11 sera derived from african burkitt lymphoma or nasopharyngeal carcinoma patients and in the corresponding serum fractions retained above xm 100 diaflo membranes after low ph treatment, and after recombination of the retained and passed fractions by neutralization of the acidified samples. they were compared with the corresponding antimembrane antigen (ma) titers in seven of the same sera. while all sera tested showed substantial increase of t ... | 1976 | 2559 |
| the specificity of heterophil antibodies in patients and healthy donors with no or minimal signs of infectious mononucleosis. | over several years sera were collected from 14 heterophil-positive students or patients who did not fulfill minimal hematologic criteria for infectious mononucleosis (i.m.) the specificity of these heterophil reactions for i.m. was investigated by determining antibodies to epstein-barr virus-determined antigens, i.e., to viral capsid antigens (vca), early antigens (ea), and ebv-associated nuclear antigens (ebna). on the basis of detectable anti-ea and/or the early absence and late emergence of a ... | 1976 | 1126 |
| the association between undifferentiated nasopharyngeal carcinoma and epstein-barr virus shown by correlated nucleic acid hybridization and histopathological studies. | all of 51 undifferentiated carcinomas of the nasopharynx were ebv-dna-positive and, in the few tests performed, ebna-positive as well. most of these patients also had high anti-ebv [vca and ea (d)] antibody titres. of four patients that had somewhat differentiated npcs, two were ebv-dna-positive with a corresponding serological picture, whereas two were negative with an ebv serology comparable to that of healthy donors. of seven lymphomas localized in the nasopharynx, one was ebv-dna-positive an ... | 1978 | 83285 |
| biology and virology of the human malignant lymphomas: 1st milford d. schulz lecture. | permanent cell lines have been established from twelve diffuse histiocytic lymphomas (su-dhl-1 to -12), three american burkitt's lymphomas (su-amb-1 to -3), two acute lymphoblastic leukemias (su-all-1 and -2), and three diffuse undifferentiated lymphomas (su-dul-1, -2, and -3). the cultured cells displayed neoplastic characteristics, as manifested by heterotransplantability in congenitally athymic nude mice and by the presence of cytogenetic abnormalities in early passage generations. functional ... | 1979 | 83902 |
| characterization of a leukemic cell line of the pre-b phenotype. | | 1979 | 83966 |
| infection with e.b. virus and rheumatoid arthritis. | | 1979 | 84110 |
| meralgia paraesthetica and epstein-barr virus. | | 1977 | 67330 |
| separation of the complement-fixing and early antigens from epstein-barr soluble antigen. | the epstein-barr virus-associated complement-fixing (soluble) antigen (cfsa) and the early antigen (ea) were isolated by ion-exchange chromatography from the p3hr-1 lymphoblastoid cell line activated with 5-iodo-2'-deoxyuridine for 72 hours. as expected, these two antigens were not immunologically identical. the cfsa, but not the ea, was isolated from the rajl lymphoblastoid cell line by the same method. | 1977 | 66320 |
| epstein-barr virus infections in the x-linked recessive lymphoproliferative syndrome. | prospective studies demonstrated variable phenotypic expression of the x-linked recessive lymphoproliferative syndrome (x.l.r.l.s.) in three brothers: (1) hypogammaglobulinaemia and subclinical epstein-barr-virus (e.b.v.) infection with antibody response to e.b.v.; (2) e.b.v. infection with defective immune response to e.b.v., fatal infectious mononucleosis (i.m.), and immunoblastic lymphoma; and (3) histiocytic lymphoma. hypogammaglobulinaemia and measles pneumonitis had preceded infection with ... | 1978 | 85816 |
| false-positive serology in infectious monoucleosis. | | 1979 | 85957 |
| x-linked immunocompetence and epstein-barr virus oncogenesis. | | 1979 | 86133 |
| cell-mediated immunity to epstein-barr virus in nasopharyngeal carcinoma. | | 1979 | 86139 |
| prospective study of epstein-barr virus antibody tetres and survival in patients with nasopharyngeal carcinoma. | | 1977 | 67401 |
| adenovirus infection and heterophile antibody production. | | 1979 | 86693 |
| a novel surface antigen on lymphoid cells transformed by epstein-barr virus. | a surface antigen (sa) was detected on ebv-carrying lymphoid cell lines by an indirect membrane immunofluorescence test with serum from rabbits immunized with raji cells; the antiserum had been extensively absorbed with normal human blood and tonsil cells. the sa was not detected on normal human umbilical-cord and adult peripheral blood lymphocytes or ebv-negative cell lines. the incidences of the sa and ebv-determined membrane antigen (ma) on certain ebv-carrying cell lines were not compatible. ... | 1978 | 87368 |
| limited immunodeficiency. | | 1978 | 87559 |
| some progress with nasopharyngeal carcinoma. | | 1979 | 87621 |
| evidence for antigenic distinctness of the epstein--barr virus-determined nuclear antigen and the herpesvirus papio-determined nuclear antigen. | previous serological evidence indicated that epstein--barr virus-determined nuclear antigen (ebna) has cross reactive components with the herpesvirus papio-determined nuclear antigen (hupna) and, in addition, it has distinct components of its own, not present in hupna. in the present study, this hypothesis was studied by absorption experiments. absorption of anti-ebna positive human sera with ebna abolished both anti-ebna and anti-hupna reactivity. absorption with hupna removed all anti-hupna re ... | 1979 | 88257 |
| quantitation of beta2-microglobulin and hla on the surface of human cells. ii. in vitro cell lines and their hybrids. | beta2-microglobulin (beta2m) participates as an integral part in molecules of the major histocompatibility complex (mhc) type. absence of beta2m makes the residual heavy mhc chain largely inactive as antigen. striking reductions in the density per unit surface area of beta2m in seven out of nine malignant lymphoid tumour lines in comparison with normal lymphocytes or "immortalized" epstein-barr-virus-transformed lymphoblastoid lines were found is this study. this would seemingly represent a spec ... | 1977 | 67633 |
| the antiherpesvirus action of phosphonoacetate. | | 1979 | 88744 |
| search for epstein-barr and type c oncornaviruses in systemic lupus erythematosus. | lymphoblastoid cell lines were derived from patients with active systemic lupus erythematosus by allowing spontaneous transformation of peripheral b lymphocytes (b cells) harboring endogenous epstein-barr virus or by superinfecting peripheral lymphocytes with exogeneous epstein-barr virus. results of extensive studies aimed at identifying type c oncornaviruses in these lymphoblastoid cells were entirely negative by electron microscopy, dna-dna hybridization, reverse transcriptase assays, and coc ... | 1979 | 88943 |
| characterization of ebv-genome negative "null" and "t" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-hodgkin lymphoma. | sixty-two explants from peripheral blood, bone marrow and cerebral fluid of children with acute lymphoblastic leukemia (all) and leukemic transformed non-hodgkin lymphoma (nhl) were cultivated for at least 8 weeks. although lymphatic cells persisted up to 16 weeks in tissue culture, no proliferation was observed in 54 cultures. from the remaining cultures, eight permanently growing cell lines were obtained. five of these were ebna (epstein-barr virus-specific nuclear antigen)-positive. three, ho ... | 1977 | 68013 |
| antibody-dependent cellular cytotoxicity (adcc) against epstein-barr virus-determined antigens. iii. reactivity in sera from patients with burkitt's lymphoma in relation to tumour development. | sera from patients with burkitt's lymphoma (bl) were tested for antibody-dependent cellular cytotoxicity (adcc) against epstein-barr virus (ebv) determined antigens and for antibodies directed against various ebv-specific antigens. no correlation was found between ebv serology and adcc in sera selected for high or low titres against the membrane antigens (ma) or in consecutive sera from three patients with isolated late tumour recurrences. furthermore, no correlation was found between adcc and t ... | 1977 | 70292 |
| perinatal infection by epstein-barr virus. | | 1979 | 89313 |
| thymidine sensitivity of cultured leukaemic lymphocytes. | cultured leukaemic lymphocytes from patients with t, null, or pre-b acute lymphoblastic leukaemia are shown to be highly sensitive to growth inhibition by thymidine. thymidine sensitivity was correlated with reduced activity of the catabolic enzyme thymidine phosphorylase and sustained elevation of the deoxythymidine triphosphate pool after exposure to thymidine. it is suggested that thymidine may have a role in the management of certain acute lymphoblastic leukaemias of lymphomas. | 1979 | 89451 |
| reciprocal 8;14 translocation in ebv-negative b-cell acute lymphocytic leukemia with burkitt-type cells. | a cytogenetic study, including prophase-prometaphase chromosome analysis, of a patient with ebv-genome-negative acute lymphocytic leukemia of b-cell type with burkitt-type cells is presented. all bone-marrow mitoses examined had a 14q+ marker chromosome which was identified as a reciprocal 8;14 translocation of exactly the same type as in burkitt's lymphoma. | 1979 | 90020 |
| cytomegalovirus-directed lymphocyte reactivity in healthy adults tested by a cmv-induced lymphocyte transformation test. | a cytomegalovirus (cmv) induced lymphocyte tranformation test (ltt) performed as a microtechnique assay is described. investigations were done in a group of twenty-five healthy adults with normal lymphocyte reactivity to phytohaemagglutinin and previously encountered antigens. lymphocyte reactivity was established in cmv-seropositive persons using heat-inactivated cell-free cmv strain ad 169 in an optimal stimulating dose of 10(1-5) tcid 50. positive cmv-ltt results were shown to be induced by p ... | 1977 | 70294 |
| site of epstein-barr virus replication in the oropharynx. | cells and cell-free material in saliva, parotid secretions, and throat washings from patients with acute infectious mononucleosis and patients undergoing tonsillectomy were assayed for the presence of infectious epstein-barr (ebv) virus. the agent was invariably present in cell-free form in saliva; neither infectious virus nor viral antigens were found in the cells. tonsillar lymphocytes from eight patients were also free of ebv. in 10 of 40 patients virus was recovered in secretions from paroti ... | 1979 | 91888 |
| establishment of a lymphoblastoid cell line and isolation of an epstein-barr-related virus of gorilla origin. | a b-lymphoid cell line was established from a normal gorilla. the cells contained epstein-barr virus-related antigens, and herpesvirus particles were demonstrated by electron microscopy. dna-dna reassociation kinétics revealed 30 to 40% hybridization to epstein-barr virus with 50 genomes per cell. examination of the viral nuclear antigen with gorilla sera showed this to be a unique isolate termed herpesvirus gorilla. h. gorilla transformed gibbon b-lymphocytes in vitro. | 1979 | 92573 |
| terminal deoxynucleotidyl transferase activity and cell surface antigens of two unique cell lines (nalm-1 and balm-2) of human leukemic origin. | two unique cell lines, nalm-1 and balm-2 derived from lymphoblast-like cells of chronic myelogenous leukemia and rare b cell acute lymphoblastic leukemia patients, respectively, were compared with fresh parent cells from the patients and with a philadelphia chromosome positive k-562 cell line previously established from a chronic myelogenous leukemia patient in blastic phase. nalm-1 resembled the parent cells in the presence of philadelphia chromosome, non-t/non-b acute lymphoblastic leukemia sp ... | 1977 | 70413 |
| continuous lymphoblastoid suspension cultures from cells of haematopoietic organs of baboons with malignant lymphoma. report iii: immunological studies. | lymphoblastoid cultures established from haematopoietic organs of baboons with lymphoma belong to the b-cell type. cytoplasmic antigen is revealed in 2--6% of suspension culture cells established from lymphomatous baboons by indirect immunofluorescence reaction using serum from human burkitt's lymphoma patients with antibody titer to capsid antigen of epstein-barr virus 1: 320. there is a correlation between the number of antigen-containing cells in these cultures and the number of cells in whic ... | 1977 | 71245 |
| [epstein-barr virus associated burkitt's lymphoma in a french child (author's transl)]. | pathologic diagnosis of malignant lymphoma, burkitt type, was made in a 12 year old child with an abdominal tumour. the association of this lymphoma with epstein-barr virus was done based on the detection of epstein-barr viral markers within the tumour cells, as well as on the patient's serology. to our knowledge, it is the first time that the association between burkitt's lymphoma and epstein-barr virus, which is frequent in africa, is reported in a caucasian french. | 1979 | 93275 |