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in vitro response of chloroquine-resistant plasmodium falciparum to mefloquine.the present study was conducted to evaluate the application of the in vitro microtechnique system in determining the response of chloroquine-resistant plasmodium falciparum to mefloquine.using isolates of p. falciparum from boa vista, brazil, and villavicencio, colombia, mefloquine was more than 7.7, 7.1, 7.1, and 6.4 times more effective than chloroquine in vitro at the ed(90), ed(95), ed(99), and ed(99.9) levels, respectively.1979393426
salmonella 38:g,z51:-, a new salmonella of sub-genus iv: a serological study on the complex h-antigen g,z51 which can be divided into three sub-genus specific factors.a new salmonella of sub-genus iv with the antigen formula 38:g,z51:- has been isolated from a boa. comparative studies of the h:g,z51 revealed that this phase only occurs in the sub-genera i, iii and iv but not in sub-genus ii. furthermore it became evident that there exist typical serological differences between the g,z51 phases of salmonella sub-genus i, iii and iv which are of significance for the differential diagnosis.1979475218
ulcerative stomatitis and subcutaneous granulomas caused by mycobacterium chelonei in a boa constrictor. 19863505951
hepatozoon fusifex sp. n., a mehogregarine from boa constrictor producing marked morphological changes in infected erythrocytes. 19694980775
respiratory and digestive lesions caused by salmonella arizonae in two snakes.cases of diphtheritic necrotizing gastritis in a rosy boa (lichanura trivirgata) and of necrotizing tracheitis in a double-headed kingsnake (lampropeltis hondurensis), both associated with salmonella arizonae, are described. an immunoperoxidase technique indicated that s. arizonae played a role in the causation of the lesions. in addition, the study showed the value of the technique for the detection of s. arizonae in the tissues of infected snakes.19968910746
predation on hapalemur griseus griseus by boa manditra (boidae) in the littoral forest of eastern madagascar. 19989885347
population genetic structure of a three-host tick, amblyomma dissimile, in eastern venezuela.patterns of genetic variation for the tick amblyomma dissimile were analyzed from a total of 200 ticks collected on 12 toads (bufo marinus), 14 snakes (boa constrictor), and 8 lizards (iguana iguana) at 11 localities. the analyses were performed on electrophoretic data from 8 isozyme loci. mean heterozygosity per locus was 6% (+/-3.1) per population. differences in allelic frequencies among ticks from different individual hosts were the major source of genetic variability in this study. host spe ...19989920303
detoxification of benzoxazolinone allelochemicals from wheat by gaeumannomyces graminis var. tritici, g. graminis var. graminis, g. graminis var. avenae, and fusarium culmorum.the ability of phytopathogenic fungi to overcome the chemical defense barriers of their host plants is of great importance for fungal pathogenicity. we studied the role of cyclic hydroxamic acids and their related benzoxazolinones in plant interactions with pathogenic fungi. we identified species-dependent differences in the abilities of gaeumannomyces graminis var. tritici, gaeumannomyces graminis var. graminis, gaeumannomyces graminis var. avenae, and fusarium culmorum to detoxify these allelo ...19989647804
[study of the phlebotomines (diptera, psychodidae), in area of cutaneous leishmaniasis in the state of mato grosso do sul, brazil].studies of the phlebotomine sandflies on the boa sorte farm, corguinho country, state of mato grosso do sul, central-west region of brazil, were carried out, with the object of identifying local fauna and a cutaneous leishmaniasis vector. at the beginning of the studies, several types of primitive vegetation covering: gallery forest, forest slopes and the cerrados: s. str. and tropical xeromorphic semideciduous broadleaf forest, locally denominated "croa", existed. four months after the beginnin ...19969077009
[dengue and its vectors in brazil].history of dengue in brazil is covered from the first citations in the xixth century to the great outbreaks of the last ten years. den-1 and den-4 viruses have been isolated for the first time in 1982 during an epidemic in boa vista, roraima state. in 1986-1987, epidemics of dengue type 1 covered an extended area from rio de janeiro/sào paulo states to the north east states of brazil. during 1990-1991, dengue type 2 epidemics have been notified in the south east (rio de janeiro/são paulo) and in ...19968924771
caveat surgeon: do orthopaedic surgeons take adequate precautions against blood-borne viral infections, in particular the human immunodeficiency virus (hiv)?one year ago the british orthopaedic association issued guidelines for the prevention of cross-infection with special reference to hiv and the hepatitis viruses. we were interested to establish whether the guidelines were being widely applied and whether they had changed general orthopaedic practice. we distributed a questionnaire to scottish orthopaedic fellows, associates of the boa, and orthopaedic trainees. with a 70 per cent return rate, it would appear that the recommendations are not adhe ...19938244540
[phlebotomines in an area of transmission of tegumentar leishmaniasis in the northern region of parana state, brazil: seasonal variation and nocturnal activity].in two years of phlebotomine captures in a modified forest an appreciable decrease in the size of this insect population has been observed when compared to previous data from the same locality. the captures with the shannon light trap were made in the outlying forest of palmital farm in terra boa county, parana state, brazil. the predominant species were lutzomyia withmani, lutzomyia migonei, lutzomyia intermedius e lutzomyia fischeri. these insects were active from 6 p.m. to 6 a.m. and the peri ...19938115833
factors conditioning the habitat and the density of biomphalaria tenagophila (orbigny, 1835) in an isolated schistosomiasis focus in rio de janeiro city.the present work was carried out in a watercress garden in alto da boa vista, in the city of rio de janeiro, brazil. the investigation was carried out in two phases. the first one (1985-86) involved the sampling of biomphalaria tenegophila in two areas to determine its relative populational densities. the results showed that the populations presented similar densities and dynamics. the second phase (1988-89) involved the study of the influence of some environmental factors on the establishment o ...19938107608
[epidemiologic study on tegumentary leishmaniasis in the municipality of corguinho, mato grosso do sul -- studies in the human population].a study on the resident population of 150 inhabitants of boa sorte in the municipality of corguinho, mato grosso do sul state, brazil was made, from march 1991 to march 1994, to establish the prevalence of south american cutaneous leishmaniasis (sacl), and to characterize the affected population, in an area of recent transmission. twelve of the inhabitants showed lesions suspected to be sacl, and in 8 cases it was possible to confirm this by biopsy and parasitology. the mucosal form was found in ...19957480911
improvement in the relationship between flow to ischemic myocardium and the extent of necrosis with glycolytic intermediates that decrease blood oxygen affinity in dogs.reducing blood oxygen affinity may enhance myocardial oxygen delivery during ischemia. we evaluated this hypothesis in awake, previously instrumented dogs that received a 20 ml/kg infusion of a solution of dihydroxyacetone, phosphate, and pyruvate after acute occlusion of either the left anterior descending or circumflex coronary artery. this infusion reduced blood oxygen affinity (boa) after 2 hours; the p50 increased from 29.9 +/- 0.7 torr (mean +/- sd) to 32.1 +/- 0.6 torr; p less than 0.01 ( ...19817249276
the polar lipids from keratinized tissues of some vertebrates.1. the lipids from a variety of keratinized tissues from terrestrial vertebrates were extracted with chloroform:methanol and the nonpolar and saponifiable lipids were removed. the composition of the remaining polar lipids was examined by thin layer chromatography and color reactions. 2. the tissues studied included: hair from a monkey (macacca fascicularis), dog (canis familiaris), raccoon (procyon lotor) and cow (bos taurus); full-thickness epidermis from a pig (sus scrofa) and pilot whale (glo ...19827172624
partial characterization of retroviruses from boid snakes with inclusion body disease.to characterize retroviruses isolated from boid snakes with inclusion body disease (ibd).200111212031
detoxification of corn antimicrobial compounds as the basis for isolating fusarium verticillioides and some other fusarium species from corn.the preformed antimicrobial compounds produced by maize, 2,4-dihydroxy-7-methoxy-2h-1,4-benzoxazin-3-one and its desmethoxy derivative 2,4-dihydroxy-2h-1,4-benzoxazin-3-one, are highly reactive benzoxazinoids that quickly degrade to the antimicrobials 6-methoxy-2-benzoxazolinone (mboa) and 2-benzoxazolinone (boa), respectively. fusarium verticillioides (= f. moniliforme) is highly tolerant to mboa and boa and can actively transform these compounds to nontoxic metabolites. eleven of 29 fusarium s ...200111425710
[dengue outbreak in boa vista, roraima. preliminary report]. 19836879054
the life history of hepatozoon rarefaciens (sambon and seligmann, 1907) from drymarchon corais (colubridae), and its experimental transfer to constrictor constrictor (boidae). 19674383539
[case of pseudomonas aeruginosa infection in tropical snakes].microbiologic and morphologic studies were carried out with there tropical snakes (two boa constrictor and one pithon molurus) that contracted the disease and died. pseudomonas aeroginosa was the only organism isolated from the affected portions of the oral cavity and the lung. it was found that all strains of the species isolated were sensitive to gentamycin, tobramycin, and polymixin b. up to their death two of the animals were treated with tobramycin with no curative effect whatever. the morp ...19853936254
disease patterns in the detroit zoo: a study of reptilian and amphibian populations from 1973 through 1983.a retrospective study was conducted to determine disease patterns in reptilian and amphibian populations at the detroit zoo from 1973 through 1983. in the reptilian population (mean +/- sd = 285.2 +/- 28), overall annual mortality rates were 1% to 40%. mortality rates were highest in the fall months (20%) and lowest in the winter months (6%). the most frequently affected reptiles were iguana (iguana iguana), reticulated python (python reticulatus), rattlesnakes (crotalus spp), common boa (constr ...19853878352
identification of major lipids from the scent gland secretions of dumeril's ground boa (acrantophis dumerili jan) by gas chromatography-mass spectrometry.the scent gland secretions of dumeril's ground boa (acrantophis dumerili), pooled from two adult males and a female, were analyzed by gas chromatography-mass spectrometry. 2-hydroxy-propanoic acid, hexadecanoic acid, cis-9-octadecenoic acid, octadecanoic acid, cholesterol, and 5-cholesten-3-one were indicated. these results are compared with those obtained in analyses of the scent gland secretions of other snakes.19883245880
morphologic and morphometric analysis of hepatozoon spp. (apicomplexa, hepatozoidae) of snakes.hepatozoon species are the most abundant hemoparasites of snakes. its identification has been based mainly on the morphologic characterization of the gamonts in the peripheral blood of the vertebrate host and also of the cysts found in the internal organs of the vertebrate and invertebrate hosts. using a computerized image analysis system, we studied five species of hepatozoon from recently captured snakes in botucatu, state of s o paulo, brazil, to evaluate the importance of the morphology and ...200312563486
inhibition of energy metabolism by benzoxazolin-2-one.the effects of the title compound (boa) on energy-linked reactions in mitochondria were studied. boa inhibited electron transfer between the flavin and ubiquinone in complex i, and atp synthesis at the f1 moiety of the atpase complex. these results are discussed in relation to the toxicity of boa towards a wide range of aerobic organisms.19872956049
identification of intermediate and branch metabolites resulting from biotransformation of 2-benzoxazolinone by fusarium verticillioides.detoxification of the maize (zea mays) antimicrobial compound 2-benzoxazolinone by the fungal endophyte fusarium verticillioides involves two genetic loci, fdb1 and fdb2, and results in the formation of n-(2-hydroxyphenyl)malonamic acid. intermediate and branch metabolites were previously suggested to be part of the biotransformation pathway. evidence is presented here in support of 2-aminophenol as the intermediate metabolite and 2-acetamidophenol as the branch metabolite, which was previously ...200312788712
study of a population of biomphalaria tenagophila (orbigny, 1835) and of schistosomiasis transmission in "alto da boa vista", rio de janeiro.the present study was performed using data from a biomphalaria tenagophila population located in a watercress garden in the alto da boa vista region representing an isolated focal point of schistosomiasis in the city of rio de janeiro. the density and age structure of this b. tenagophila population and its rate of infection by schistosoma mansoni were studied for a period of 15 months. the snail population showed seasonal variation in density, with a decrease in number of individuals at the begi ...19892520827
inhibition of human fibroblast growth in vitro by a snake oil.the inhibitory effects of boa constrictor fat (bcf) oil on the growth kinetics of keloid and normal dermal fibroblasts were tested in fibroblasts cultures. bcf significantly (p less than 0.0001) inhibited the in vitro growth of both keloid and normal dermal fibroblasts. although the active ingredient(s) in this snake oil is not yet determined, it is postulated that fatty acids which are the main constituents of the oil may in part account for this observed in vitro effect.19902328380
[identification of a new focus of schistosoma mansoni. (municipality of nova lima, mg, brazil].the detection of a new transmission focus of schistosomiasis in nova lima town, minas gerais state, brazil, is reported. the search of snail intermediate hosts in banqueta do bananal a small locality of boa vista neighbourhood detected 356 specimens of biomphalaria glabrata, with a infection rate of 8.4%. in hundred-sixty-two patients stool examinations (kato-katz method) were done, showing ninety-one patients with eggs of schistosoma mansoni in their feces. the integrated action of local reside ...19911842844
lead-210 and polonium-210 concentrations in some species of marine molluscs.data are presented for 210pb and 210po concentrations in three species of molluscs collected in the intertidal region of the coast of rio de janeiro, brazil. lead-210 activity in the soft tissue of the edible mussel perna perna was 20 times higher than that found in its shell. the visceral mass contained the largest activity for both radionuclides, which is attributed to the feeding habits of the molluscs. animals collected from ponta negra beach (open sea) showed higher concentrations of isotop ...19921566047
a parvovirus isolated from royal python (python regius) is a member of the genus dependovirus.parvoviruses were isolated from python regius and boa constrictor snakes and propagated in viper heart (vh-2) and iguana heart (igh-2) cells. the full-length genome of a snake parvovirus was cloned and both strands were sequenced. the organization of the 4432-nt-long genome was found to be typical of parvoviruses. this genome was flanked by inverted terminal repeats (itrs) of 154 nt, containing 122 nt terminal hairpins and contained two large open reading frames, encoding the non-structural and ...200414993638
epizootic of fatal amebiasis among exhibited snakes: epidemiologic, pathologic, and chemotherapeutic considerations.an epizootic of reptilian amebiasis seems to have caused the death of 15 to 16 large and valuable captive snakes (boas, pythons, and anacondas) occupying one of 5 large display dioramas in the steinhart aquarium of the california academy of science, golden gate park, san francisco. subsequent review of previous snake deaths in the colony indicated that of 464 snakes that had died since early 1969, 89 snakes had intestinal or hepatic lesions, and 80 of these snakes had pathologic features which i ...19751147335
arizona septicemia in three boa constrictors. 19751041820
reduction of human blood o2 affinity using dihydroxyacetone, phosphate, and pyruvate.human blood oxygen affinity (boa) was measured after blood from six normal donors was incubated with 4 concentrations of dihydroxyacetone (0.022, 0.044, 0.088, and 0.175 m) plus equimolar disodium phosphate and pyruvate (sodium salt) (0.013, 0.025, 0.05 and 0.1 m) in solutions labeled ddp x 1, ddp x 2, ddp x 4, and ddp x 8, respectively. blood p50 rose (boa was reduced) from a control value of 26.0 +/- 0.4 torr (mean +/- sd) to 29.4 +/- 0.6, 30.6 +/- 0.4, 31.9 +/- 0.15 and 33.3 +/- 1.4 torr afte ...1979118143
[impact of environmental changes on sand fly ecology in southern brazil].tree felling around a chicken coop and periodic insecticide spraying at the palmital farm in the county of terra boa in southern brazil impacted the ecology of sand fly species. the most prevalent species (51.6%) before clearing the trees and insecticide spraying was lutzomyia migonei (frança, 1920), and it is now lutzomyia (nyssomyia) whitmani (antunes & coutinho, 1939) (89.8%). there was a highly significant difference between the proportions of l. whitmani reported by this paper (89.8%) and a ...199910633213
analysis of its2 dna sequences from brazilian anopheles darlingi (diptera: culicidae).specimens of anopheles darlingi root, the major vector of malaria in brazil, were collected from several states in brazil: sao paulo (dourado), bahia (itabela), rondônia (porto velho), roraima (boa vista), and acre (plácido de castro). sequence divergence in the 2nd internal transcribed spacer (its2) was examined. the its2 sequences of mosquitoes captured in the amazon region (porto velho, boa vista and plácido de castro) and in the northeast of brazil (itabela) were almost identical; however, a ...199910534960
[the laboratory diagnosis and epidemiology of cutaneous leishmaniasis in paraná state, southern brazil].between 1986 and 1997 a total of 1418 patients were examined at the clinical analysis teaching and research laboratory of maringá state university (lepac/uem) for cutaneous leishmaniasis by direct examination of stained smears made from the lesions, the montenegro skin test and the indirect immunofluorescent antibody test. nine hundred and fifty five patients (67.3%) were positive for at least one of the three tests and of these 804 (84.2%) were considered to have contracted the disease in paran ...199910495672
ticks parasitizing reptiles in the bahamas.two species of reptile ticks, amblyomma dissimile koch and amblyomma torrei pérez vigueras (acari: ixodidae), are reported from the bahama islands for the first time. the widespread neotropical (including the caribbean and southern florida) a. dissimile was recovered on andros island from three species of reptiles all for the first time: the andros iguana cyclura cychlura cychlura cuvier, the andros curly tail lizard leiocephalus carinatus coryi schmidt, and the andros boa epicrates striatus fow ...200516134982
[mass production of romanomermis iyengari (nematoda: mermithidae) applied to anopheline breeding sites in boa vista (roraima), brazil].in order to begin mass producing the nematode romanomermis iyengari welch, a bioprocessing plant was designed and set up on the grounds of universidad federal in the state of roraima, brazil, after reaching an agreement with the state's health department. the objective of this paper was to establish the basic process for mass breeding the parasite in order to apply it to anopheline breeding sites. cultures were obtained at a rate of 68 during every seven-day cycle, making for a total of 272 cult ...200010808748
[malaria in the region of campinas, são paulo, brazil, 1980 to 1994].according to epidemiological data, from 1980 to 1994, 2,781 cases of malaria were registered as follows: dir xii-campinas (49.3%), dir xv-piracicaba (41.3%) and dir xx-são joão da boa vista (9.4%). the plasmodium vivax was found in 70.6% of the patients; plasmodium falciparum in 25.4% and 4% of the patients presented a mixed infection. the epidemiological classification showed that 95% of the cases came from the states of rondônia, mato grosso and par. the masculine sex, between the age of 20 to ...200010881119
[serologic survey of dogs in an endemic area of tegumentary leishmaniasis in paraná state, southern brazil]increased reporting of mucocutaneous leishmaniasis in the state of paraná points to the need for information on both this disease's epidemiology and pertinent control measures. a serological survey was thus performed, with indirect immunofluorescence (iif) for canine leishmaniasis in farm operations belonging to the companhia melhoramentos norte do paraná, in jussara and terra boa counties, in northwestern paraná. iif was performed on 132 dogs, of which 24 (18.2%) had significant titers (>/=40). ...199610904311
[epidemiologic aspects of cutaneous leishmaniasis in an endemic area of the state of paraná, brazil]an epidemiological investigation of cutaneous leishmaniasis (cl) was performed in 1992 and 1993 in an endemic area of jussara and terra boa counties, in the northern paraná state. the study covered 684 individuals from a population of approximately 1,400 from six agricultural areas (fazenda palmital, cerâmica andirá, fazenda jussara, fazenda lagoa, destilaria melhoramentos, and fazenda mururê) in northern paraná, brazil. the mean frequency of cl history was 19.9%. the highest frequency was at fa ...199610904316
the evolution of loss of heterozygosity on chromosome 17 during the progression to barrett's adenocarcinoma involves a unique combination of target sites in individual specimens.we have previously identified thirteen common minimally deleted regions (mrs) on chromosome 17 in twelve barrett's esophageal adenocarcinoma (boa) specimens using 41 precisely mapped microsatellite markers (dunn et al., oncogene, 17: 987-993, 1999). the aim of the present study has been to identify the earliest sites of loss on this chromosome that arise and persist during the progression to boa. this has been undertaken by the analysis of multiple carefully microdissected tissue samples from ea ...200011051253
early determination of the reproductive number for vector-borne diseases: the case of dengue in brazil.to evaluate a new method of deriving the reproductive number for vector-borne diseases from the early epidemic curves for vector-borne diseases with incubations in the vectors and in the hosts.200616553913
isolation and characterization of an antigenically distinct 68-kd protein from nonviral intracytoplasmic inclusions in boa constrictors chronically infected with the inclusion body disease virus (ibdv: retroviridae).the relationship between a retroviral infection and the development of nonviral intracytoplasmic inclusion bodies was studied in a boa constrictor model. twelve juvenile age- and size-matched inclusion body disease (ibd)-negative boas were randomly divided into three groups. each group was inoculated intraperitoneally with 1 ml of an ibd virus (ibdv)-infected liver homogenate or 1 ml of normal boa liver homogenate (sham-inoculated control) or was left untreated. all boas were monitored for devel ...200011055868
[focal and total residual insecticide spraying to control triatoma brasiliensis and triatoma pseudomaculata in northeast brazil].to efficiently control the triatomines triatoma brasiliensis and triatoma pseudomaculata, a field trial was performed to compare conventional versus focal spraying of deltamethrin 5% sc at 25 mg a. i./m2 and the slow-release organophosphate malathion 8.3% sr at 2g a. i./m2. the assay took place in the county of boa viagem, ceará state, with 1541 households, randomly separated into 4 groups. two of them received focal spraying: pt, treated with deltamethrin indoors and in the peridomicile, and pl ...200011119328
the life-cycle and ultrastructure of sarcocystis ameivamastigodryasi n. sp., in the lizard ameiva ameiva (teiidae) and the snake mastigodryas bifossatus (colubridae).sarcocysts in muscles of the teiid lizard ameiva ameiva from north brazil were fed to the colubrid snake mastigodryas bifossatus, the faeces of which had been shown to be devoid of coccidial oocysts or sporocysts. when necropsied 16 days later the snake was shown to have developed a massive intestinal infection of sarcocystis. mature sporocysts from another, naturally infected m. bifossatus were fed to juvenile specimens of a. ameiva in which no sarcocysts could be detected in tail muscle biopsi ...200011147034
novel mri contrast agent for molecular imaging of fibrin: implications for detecting vulnerable plaques.molecular imaging of thrombus within fissures of vulnerable atherosclerotic plaques requires sensitive detection of a robust thrombus-specific contrast agent. in this study, we report the development and characterization of a novel ligand-targeted paramagnetic molecular imaging agent with high avidity for fibrin and the potential to sensitively detect active vulnerable plaques.200111551880
effect of 2-benzoxazolinone (boa) on seedling growth and associated biochemical changes in mung bean (phaseolus aureus).boa (2-benzoxazolinone) is a potent phytotoxin present in several graminaceous crops such as rye, maize and wheat. due to its wide range of phytotoxicity, it is considered as a potential pesticide. a study was conducted to explore the impact of boa on the radicle and plumule elongation of mung bean (phaseolus aureus) and associated changes in the macromolecular content - proteins and carbohydrates - and activities of enzymes like amylases, proteases, polyphenol oxidases and peroxidases. boa sign ...200617137118
light microscopical structure and ultrastructure of a besnoitia sp. in the naturally infected lizard ameiva ameiva (teiidae) from north brazil, and in experimentally infected mice.a besnoitia species of the teiid lizard ameiva ameiva (l.), from north brazil was established in laboratory mice and hamster by the intraperitoneal inoculation of bradyzoites in the tissue cysts. in the lizards all the cyst wall layers were closely apposed. in the mice the layers of the wall were distinguishable, and ultrastructurally the inner cytoplasmic layer contained either a tight network of endoplasmic reticulum or packed mitochondria or both. these components were less frequent or sparse ...200111578088
fdb1 and fdb2, fusarium verticillioides loci necessary for detoxification of preformed antimicrobials from corn.fusarium verticillioides is a fungus of significant economic importance because of its deleterious effects on plant and animal health and on the quality of their products. corn (zea mays) is the primary host for f. verticillioides, and we have investigated the impact of the plant's antimicrobial compounds (dimboa, diboa, mboa, and boa) on fungal virulence and systemic colonization. f. verticillioides is able to metabolize these antimicrobials, and genetic analyses indicated two loci, fdb1 and fd ...200211876429
seasonal influence on hematologic values and hemoglobin electrophoresis in brazilian boa constrictor amarali.as ectothermic animals, snakes depend exclusively on the environment for proper temperature maintenance, which may greatly influence their activity. twenty-five adult boa constrictor amarali snakes maintained in captivity were used to determine the influence of seasons on their hematologic values and electrophoretic profile of hemoglobin. a complete blood cell count (cbc) and examination for hemoparasites were performed in the summer and winter of 2004. hemoglobin was stored for later electropho ...200617315433
biting indices, host-seeking activity and natural infection rates of anopheline species in boa vista, roraima, brazil from 1996 to 1998.the epidemiology of the transmission of malaria parasites varies ecologically. to observe some entomological aspects of the malaria transmission in an urban environment, a longitudinal survey of anopheline fauna was performed in boa vista, roraima, brazil. a total of 7,263 anophelines was collected in human bait at 13 de setembro and caranã districts: anopheles albitarsis sensu lato (82.8%), an. darlingi (10.3%), an. braziliensis (5.5%), an. peryassui (0.9%) and an. nuneztovari (0.5%). nightly 1 ...200212016435
biotransformation of 2-benzoxazolinone and 2-hydroxy-1,4-benzoxazin-3-one by endophytic fungi isolated from aphelandra tetragona.the biotransformation of the phytoanticipins 2-benzoxazolinone (boa) and 2-hydroxy-1,4-benzoxazin-3-one (hboa) by four endophytic fungi isolated from aphelandra tetragona was studied. using high-performance liquid chromatography-mass spectrometry, several new products of acylation, oxidation, reduction, hydrolysis, and nitration were identified. fusarium sambucinum detoxified boa and hboa to n-(2-hydroxyphenyl)malonamic acid. plectosporium tabacinum, gliocladium cibotii, and chaetosphaeria sp. t ...200212324332
use of an elisa for detection of antibody responses in argentine boa constrictors (boa constrictor occidentalis).to develop mouse monoclonal and rabbit polyclonal antibodies against immunoglobulin of argentine boa constrictors and to demonstrate the ability of these reagents to detect antibody responses in boa constrictors by use of an elisa and western blot analysis.200312693526
physical and transcript map of the minimally deleted region iii on 17p implicated in the early development of barrett's oesophageal adenocarcinoma.allelic imbalance (ai) studies on chromosome 17 (c17) in barrett's oesophageal adenocarcinoma (boa) tumours strongly suggest that a minimally deleted region on c17p harbours a boa-associated gene with tumour suppressor function. this deleted region, designated minimal region iii (mriii), lies between the two microsatellite markers d17s1852 and d17s954. computational sequence analysis techniques, blast and nix, were used to assemble a physical map of mriii, consisting of three overlapping bacteri ...200312821948
improved molecular imaging contrast agent for detection of human thrombus.molecular imaging of microthrombus within fissures of unstable atherosclerotic plaques requires sensitive detection with a thrombus-specific agent. effective molecular imaging has been previously demonstrated with fibrin-targeted gd-dtpa-bis-oleate (boa) nanoparticles. in this study, the relaxivity of an improved fibrin-targeted paramagnetic formulation, gd-dtpa-phosphatidylethanolamine (pe), was compared with gd-dtpa-boa at 0.05-4.7 t. ion- and particle-based r(1) relaxivities (1.5 t) for gd-dt ...200312876719
ultrastructural studies on the sporogony of hepatozoon spp. in culex quinquefasciatus say, 1823 fed on infected caiman crocodilus and boa constrictor from northern brazil.laboratory reared culex quinquefasciatus say, 1823 originating from the vicinity of belem, in northern brazil, were allowed to engorge on caimans (caiman c. crocodilus) infected with hepatozoon caimani (carini, 1909) and boas (boa constrictor) infected with h. cf. terzii (sambon and seligmann, 1907) both from para state. engorged mosquitoes killed on successive days post-feeding (p.f.) were studied by transmission electron microscopy. images of oocysts from 13 days p.f., caiman-fed mosquitoes we ...200312954016
aedes aegypti in brazil: genetically differentiated populations with high susceptibility to dengue and yellow fever viruses.aedes aegypti was eliminated from brazil in 1955, but re-infested the country in the 1970s. dengue outbreaks have occurred since 1981 and became endemic in several cities in brazil after 1986. urban yellow fever has not occurred since 1942, and only jungle yellow fever cases have been reported. a population genetic analysis using isoenzyme variation combined with an evaluation of susceptibility to both yellow fever and dengue 2 viruses was conducted among 23 a. aegypti samples from 13 brazilian ...200414702837
fatal attack of a boa constrictor on a bearded saki (chiropotes satanas utahicki). 200415010586
seasonal variation of hepatozoon spp. (apicomplexa, hepatozoidae) parasitemia from boa constrictor amarali (serpentes, boidae) and hydrodynastes gigas (serpentes, colubridae).the aim of this study was to evaluate the parasitemia variation of three hepatozoon species in brazilian snakes. this study was conducted between 2001 and 2003 and included hepatozoon terzii from boa constrictor amarali, and hepatozoon migonei and hepatozoon cyclagrasi from hydrodynastes gigas. it was observed that the parasitemia tended to decrease in all three hepatozoon species but the parasites were not eliminated. this data suggest that hepatozoon infection may be similar to toxoplasma gond ...200515999279
1,4-benzoxazin-3-one, 2-benzoxazolinone and gallic acid from calceolaria thyrsiflora graham and their antibacterial activity.secondary metabolites, diboa, hboa, 7-oh-hboa, boa and gallic acid, were isolated and quantified from calceolaria thyrsiflora graham, a native medicinal plant of chile belonging to the scrophulariaceae family. the highest diboa contents were determined in leaves (145 mmol kg(-1) dry wt) and flowers (161 mmol kg(-1) dry wt). antibacterial activities of diboa, hboa, boa, gallic acid and infusions of flowers and leaves were determined. the phytomedicinal properties attributed to c. thyrsiflora grah ...200516042337
phylogeography of aedes (stegomyia) aegypti (l.) and aedes (stegomyia) albopictus (skuse) (diptera: culicidae) based on mitochondrial dna variations.aedes (stegomyia) aegypti (l.) and aedes (stegomyia) albopictus (skuse) are the most important vectors of the dengue and yellow-fever viruses. both took advantage of trade developments to spread throughout the tropics from their native area: a. aegypti originated from africa and a. albopictus from south-east asia. we investigated the relationships between a. aegypti and a. albopictus mosquitoes based on three mitochondrial-dna genes (cytochrome b, cytochrome oxidase i and nadh dehydrogenase subu ...200516181519
idiopathic bilateral optic atrophy in the rhesus macaque.to document the existence of idiopathic bilateral optic atrophy (boa) in rhesus macaque monkeys and to characterize the structural and functional consequences of this condition.200516249467
hyperparasitism in amblyomma rotundatum (acari: ixodidae).in the present study, we report a case of hyperparasitism in amblyomma rotundatum. during examination of live ticks immediately after collecting them from boa constrictor snakes held in a reptile facility in mossoró, rn, northeastern brazil, 1 unengorged tick female was seen attached to the venter of a partially engorged female. the hypostome and chelicerae of the unengorged female had penetrated the integument of the partially engorged female to the level of the basis capitulli and the palps we ...200718314708
cyanoacrylate repair of laser in situ keratomileusis corneal flap perforation by a snake bite.a 30-year-old man who had laser in situ keratomileusis (lasik) for myopia 1 year earlier developed a corneal perforation in the left eye from a boa constrictor. the patient presented to the emergency room, and a small corneal perforation just outside the visual axis was diagnosed within the lasik flap. cyanoacrylate adhesive was used to close the corneal perforation. the patient went on to full visual recovery with an uncorrected visual acuity of 20/20. this is the first reported case of a penet ...200516412943
effects of some benzoxazinoids on in vitro growth of cephalosporium gramineum and other fungi pathogenic to cereals and on cephalosporium stripe of winter wheat.the benzoxazolinones benzoxazolin-2(3h)-one (boa) and 6-methoxybenzoxazolin-2(3h)-one (mboa) and selected degradation products of these compounds were examined for their in vitro antifungal activity against cephalosporium gramineum, gaeumannomyces graminis var. graminis, and fusarium culmorum. boa was also applied to the soil-incorporated inoculum of c. gramineum to test its capability of reducing cephalosporium stripe disease in winter wheat. mboa reduced the mycelial growth of g. graminis var. ...200616478214
ecotoxicological effects of benzoxazinone allelochemicals and their metabolites on aquatic nontarget organisms.before natural plant allelochemicals can be exploited as biological pesticides against weeds and for disease control, more than the effect on target organisms needs to be known. this study presents results of aquatic biotests using four organisms, namely, a water flea, a freshwater alga, a soil alga, and a luminescent bacterium. the tested substances were 10 benzoxazinone derivatives, 3 of them known to be wheat allelochemicals, benzoxazolin-2(3h)-one (boa), 6-methoxybenzoxazolin-2(3h)-one (mboa ...200616478223
endoscopic examination of snakes by access through an air sac.sixteen boa constrictors (boa constrictor), three royal pythons (python regius) and 15 burmese pythons (python molurus bivittatus) were examined endoscopically by access through the air sac. the snakes were immobilised in a ventral position using a half-open anaesthetic system with assisted ventilation and a mixture of isoflurane and oxygen. the rigid endoscope was introduced percutaneously and the internal structure of the lungs and the air sac, and the shape, size and external surface of the l ...200616565339
early stone technology on flores and its implications for homo floresiensis.in the soa basin of central flores, eastern indonesia, stratified archaeological sites, including mata menge, boa lesa and kobatuwa (fig. 1), contain stone artefacts associated with the fossilized remains of stegodon florensis, komodo dragon, rat and various other taxa. these sites have been dated to 840-700 kyr bp (thousand years before present). the authenticity of the soa basin artefacts and their provenance have been demonstrated by previous work, but to quell lingering doubts, here we descr ...200616738657
the importance of anopheles albitarsis e and an. darlingi in human malaria transmission in boa vista, state of roraima, brazil.in several districts of boa vista, state of roraima, brazil we found anopheles (nyssorhynchus) albitarsis e to be the primary vector of human malaria parasites, and during 2001-2002 it was significantly more abundant than an. darlingi (p < 0.001). other species sampled were an. (nys.) braziliensis, an. (ano.) peryassui, an. (nys.) nuneztovari, an. (nys.) oswaldoi s.l., and an. (nys.) triannulatus. as determined by the elisa technique an. darlingi had a higher overall infection rate (2.1%) compar ...200616830709
2-benzoxazolinone (boa) induced oxidative stress, lipid peroxidation and changes in some antioxidant enzyme activities in mung bean (phaseolus aureus).2-benzoxazolinone (boa), a well-known allelochemical with strong phytotoxicity, is a potential herbicidal candidate. the aim of the present study was to determine whether phytotoxicity of boa is due to induction of oxidative stress caused by generation of reactive oxygen species (ros) and the changes in levels of antioxidant enzymes induced in response to boa. effect of boa was studied on electrolyte leakage, lipid peroxidation (lp), hydrogen peroxide (h(2)o(2)) generation, proline (pro) accumul ...200617107811
characteristics of hammer stones and anvils used by wild bearded capuchin monkeys (cebus libidinosus) to crack open palm nuts.capuchins living in boa vista (piauì, brazil) crack open hard palm nuts on hard, level surfaces (anvils) using stones (hammers) as percussive tools. this activity leaves diagnostic physical remains: distinctive shallow depressions (pits) on the surface of the anvil, cracked shells, and stone hammers on the anvil. to initiate comparison of percussive stone tool use and interpretation of the artifacts it produces across capuchins, chimpanzees, and hominins, we describe a sample of the anvils and h ...200717177182
physicochemical, antimicrobial and anti-inflammatory evaluation of fixed oil from boa constrictor.boa constrictor is one of the snakes found in the riverine areas of nigeria, especially in the niger delta regions. the fat obtained from the snake is used ethno-medicinally for the treatment of burns and inflammatory conditions. the purpose of this study was to validate the traditional use of this crude fat and oil. the fat obtained from the boa snake was subjected to some physiochemical screening tests. a systematic chemical and antimicrobial investigation was carried out using some bacterial ...200819051590
zea mays: benzoxazolinone detoxification under sulfur deficiency conditions--a complex allelopathic alliance including endophytic fusarium verticillioides.benzoxazolinone detoxification is similar in plants grown under sulfur deficiency conditions and in control plants grown with an optimal s supply. however, when s-deficient plants were treated with metolachlor before benzoxazolin-2(3h)-one (boa) incubation, detoxification was reduced, as indicated by a lower accumulation of the detoxification products boa-6-o-glucoside and glucoside carbamate and by a loss of boa-6-oh glucosyltransfease activity. root colonizing microorganisms and the endophytic ...200717200890
geographical distribution of genetically determined types of nezara viridula (l.) (heteroptera: pentatomidae) in brazil.nezara viridula (l.) is a cosmopolitan and polymorphic pentatomid. several genetically determined types have been described due to body color variation in adults. a survey covering 13 brazilian states was conducted during 2001 and 2002 to determine the geographical distribution of the main types. type g (smaragdula--body entirely green), the most common, showed a wide distribution, from south to north (latitude 2 degrees 49' n to 31 degrees 46' s), except in the central-west region. type o (torq ...200617348127
achaete-scute homolog-1 linked to remodeling and preneoplasia of pulmonary epithelium.the basic helix-loop-helix protein achaete-scute homolog-1 (ash1) is involved in lung neuroendocrine (ne) differentiation and tumor promotion in sv40 transgenic mice. constitutive expression of human ash-1 (hash1) in mouse lung results in hyperplasia and remodeling that mimics bronchiolization of alveoli (boa), a potentially premalignant lesion of human lung carcinomas. we now show that this is due to sustained cellular proliferation in terminal bronchioles and resistance to apoptosis. throughou ...200717507989
innate defensive behaviour and panic-like reactions evoked by rodents during aggressive encounters with brazilian constrictor snakes in a complex labyrinth: behavioural validation of a new model to study affective and agonistic reactions in a prey versus predator paradigm.defensive behaviour has been extensively studied, and non-invasive methodologies may be interesting approaches to analyzing the limbic system function as a whole. using experimental models of animals in the state of anxiety has been fundamental in the search for new anxiolytic and antipanic compounds. the aim of this present work is to examine a new model for the study of affective behaviour, using a complex labyrinth consisting of an arena and galleries forming a maze. furthermore, it aims to c ...200717604117
ticks infesting amphibians and reptiles in pernambuco, northeastern brazil.ticks infesting amphibians and reptiles in the state of pernambuco are reviewed, based on the current literature and new collections recently carried out by the authors. to date, three tick species have been found on amphibians and reptiles in pernambuco. amblyomma fuscum appears to be exclusively associated with boa constrictor, its type host. amblyomma rotundatum has a relatively low host-specificity, being found on toads, snakes, and iguana. amblyomma dissimile has been found on a lizard and ...200819265581
investigation of rice transgene flow in compass sectors by using male sterile line as a pollen detector.rice is the most important staple food in the world. the rapid development of transgenic rice and its future commercialization have raised concerns regarding transgene flow and its potential environmental risk. it is known that rice is a self-pollinated crop; the outcrossing rate between common cultivars is generally less than 1%. in order to improve the detection sensitivity of rice transgene flow, a male sterile (ms) line boa with a high outcrossing rate was used as a pollen detector in this s ...200717622509
[survey of cnidarian accident records in some beaches of the coast of pernambuco (brazil)].cnidarians are among the most venomous organisms known to man. they are characterized by stinging cells called cnidocytes, and several species, such as the portuguese-man-of-war and the jellyfish, can cause harm to human beings. despite not attracting () much attention on the brazilian coast, the studies that have been carried out to date show that the occurrence of this kind of accident is significant. the aim of this study was to survey cnidarian related accidents with beach goers on some beac ...200717680074
interactions of bacillus mojavensis and fusarium verticillioides with a benzoxazolinone (boa) and its transformation product, apo.the benzoxazolinones, specifically benzoxazolin-2(3h)-one (boa), are important transformation products of the benzoxazinones that can serve as allelochemicals providing resistance to maize from pathogenic bacteria, fungi, and insects. however, maize pathogens such as fusarium verticillioides are capable of detoxifying the benzoxazolinones to 2-aminophenol (ap), which is converted to the less toxic n-(2-hydroxyphenyl) malonamic acid (hpma) and 2-acetamidophenol (hpaa). as biocontrol strategies th ...200717896139
identification of chlamydophila pneumoniae in an emerald tree boa, corallus caninus.tissues were evaluated from emerald tree boas, corallus caninus, from a collection in which chlamydiosis was diagnosed. to determine the strain of chlamydia infecting these snakes, tissue samples from 5 frozen snakes were tested by a quantitative taqman polymerase chain reaction (pcr) test and a pcr sequence analysis test. of the 22 samples tested, 9 were categorized as either positive or weakly positive with the taqman test, and 6 yielded an amplicon using a serial pcr test that amplified a por ...200415053368
ticks on captive and free-living wild animals in northeastern brazil.from 2005 to 2009, 147 ticks found on 32 wild animals from or referred to two zoobotanical parks (parque zoobotânico arruda câmara and parque estadual dois irmãos) located in northeastern brazil were identified. ticks found on two veterinarians working in one of the parks (i.e., parque estadual dois irmãos), after return from forested areas within the park's territory, were also identified. the following tick-host associations were recorded: amblyomma fuscum neumann on boa constrictor l.; amblyo ...201019693679
hepatozoon cf. terzii (sambon & seligman, 1907) infection in the snake boa constrictor constrictor from north brazil: transmission to the mosquito culex quinquefasciatus and the lizard tropidurus torquatus.specimens of hepatozoon-infected boa constrictor constrictor were obtained from localities in pará state, north brazil. gametocytes in erythrocytes of the peripheral blood measured 10 x 2.5-16.2 x 3.7 microns. they were similar to those described as haemogregarina terzii by sambon & seligmann (1907) in b. c. constrictor, in that they did not distort the infected erythrocyte, and their dimensions approximated those given by carini (1947). lungs and liver of infected snakes contained actively divi ...200415224579
[perception of work conditions in a traditional farming community in boa esperança, nova friburgo, rio de janeiro state, brazil].the impact of pesticide use on human and environmental health has received attention from the international scientific community for some time, especially in developing countries where such chemical agents are used extensively and indiscriminately. the present study adopts a methodological approach to risk perception analysis based on rapid surveys, including: participatory observation; semi-structured interviews with key informants; and evaluation of local work processes. field data analysis re ...200415300299
a new species of hepatozoon (apicomplexa: adeleorina) from python regius (serpentes: pythonidae) and its experimental transmission by a mosquito vector.hepatozoon ayorgbor n. sp. is described from specimens of python regius imported from ghana. gametocytes were found in the peripheral blood of 43 of 55 snakes examined. localization of gametocytes was mainly inside the erythrocytes; free gametocytes were found in 15 (34.9%) positive specimens. infections of laboratory-reared culex quinquefasciatus feeding on infected snakes, as well as experimental infection of juvenile python regius by ingestion of infected mosquitoes, were performed to complet ...200718163356
population structure of an endemic vulnerable species, the jamaican boa (epicrates subflavus).the jamaican boa (epicrates subflavus; also called yellow boa) is an endemic species whose natural populations greatly and constantly declined since the late 19th century, mainly because of predation by introduced species, human persecution, and habitat destruction. in-situ conservation of the jamaican boa is seriously hindered by the lack of information on demographic and ecological parameters as well as by a poor understanding of the population structure and species distribution in the wild. h ...200818179440
diphtheroid colitis in a boa constrictor infected with amphibian entamoeba sp.a female boa (boa constrictor) from a zoological collection was submitted for necropsy after sudden death. prominent pathological findings included a diphtheroid colitis, endoparasitism, focal pneumonia and inclusion bodies typical for inclusion body disease (ibd). in the colon entamoebae were identified, which differed in size and distribution from entamoeba invadens. gene sequence analysis of the 18s ribosomal rna revealed 100% similarity with an entamoeba species from the african bullfrog (py ...200818291588
[effect of collect time on communities of epigaeic ants (hymenoptera: formicidae) in areas of eucalyptus cloeziana and cerrado].this study aimed at evaluating the effect of collect time (day and night) on ant fauna attracted to baits in areas of eucalyptus cloeziana (myrtaceae) and cerrado. the ants were collected in fazenda boa vista, mannesmann fi - el florestal ltda, paineiras, minas gerais state. eigthteen sample units were collected: 12 in e. cloezina and six in cerrado. each sample unit consisted of three plots (25 x 35 m each). the plot consisted of 34 baits distributed in a grid pattern at 5 m intervals. the samp ...200818506289
[geographic distribution and epidemiological features of american tegumentary leishmaniasis in old rural settlements in paraná state, southern brazil].this study analyzes the epidemiology of american tegumentary leishmaniasis in the municipalities of the central north, central west, and northwest mesoregions of paraná state, brazil. diagnosis of american tegumentary leishmaniasis cases was performed at the state university in maringá from 1987 to 2004. according to the probable site of infection, cases were classified as autochthonous (infection inside the household domain) or allochthonous (outside the household domain). municipalities with t ...200818545755
occurrence of cryptosporidium sp. in snakes in japan.the aim of this study was to determine the prevalence of cryptosporidium in snakes in japan. fecal samples or intestinal contents of 469 snakes, consisting of five species, were analyzed and cryptosporidium oocysts were detected only from the japanese grass snake rhabdophis tigrinus. the mean prevalence of cryptosporidium sp. in japanese grass snakes was approximately 26% in the region studied. histopathological observations revealed that the organism caused proliferative enteritis in the small ...200818548279
[abundance and seasonality of diptera (insecta) in a poultry house in the northeast region of the state of são paulo, brazil].modern production systems accommodate broody hens in high densities, leading to the accumulation of excrement under the cages. this substrate is excellent for the development of sinantropic flies. thus, the accomplishment of surveys in these places becomes essential, in order to plan better strategies of control. the present work aimed at studying the entomofauna and the seasonality of the species of dipterous present in the crisdan poultry house located in the municipality of são joão da boa vi ...200818554436
ticks infesting wildlife species in northeastern brazil with new host and locality records.from september 2008 to march 2010, 397 ticks (315 larvae, 33 nymphs, 23 females, and 26 males) were collected from captive and free-living wildlife species in northeastern brazil. six tick species were identified, including amblyomma auricularium (conil) on tamandua tetradactyla (l.), amblyomma dubitatum neumann on hydrochaeris hydrochaeris (l.), nectomys rattus (pelzen) and t. tetradactyla, amblyomma parvum aragão on t. tetradactyla, amblyomma rotundatum koch on boa constrictor l., chelonoidis ...201021175080
physical properties of palm fruits processed with tools by wild bearded capuchins (cebus libidinosus).habitually, capuchin monkeys access encased hard foods by using their canines and premolars and/or by pounding the food on hard surfaces. instead, the wild bearded capuchins (cebus libidinosus) of boa vista (brazil) routinely crack palm fruits with tools. we measured size, weight, structure, and peak-force-at-failure of the four palm fruit species most frequently processed with tools by wild capuchin monkeys living in boa vista. moreover, for each nut species we identify whether peak-force-at-fa ...200818618602
oviduct adenocarcinoma in some species of captive snakes.this article reports 5 cases of oviduct adenocarcinoma in adult captive snakes from smithsonian's national zoological park. this neoplasm was found in 1 of each of the following species: emerald tree boa (corallus caninus), amazonian tree boa (corallus enydris enydris), burmese rock python (python molurus bivittatus), northern pine snake (pituophis melanoleucus melanoleucus), and corn snake (elaphe guttata). grossly, tan to red firm masses were found within oviducts in 3 cases. in an additional ...200818725476
blood testosterone in rats: correlation of the level of individual anxiety and its impairment after "death threat".studies of identical groups of male wistar rats after preliminary selection to give groups including extreme behavioral types with low and high rankings on the anxiety scale showed that blood testosterone concentrations in intact rats (controls) correlated negatively with anxiety ranking, i.e., minimal hormone concentrations (no greater than 5 nm) corresponded to high levels of anxiety - with a predominance of passive defensive behavioral components on testing. short-term exposure to a "death th ...200818975099
dengue virus in aedes aegypti larvae and infestation dynamics in roraima, brazil.to detect the presence of dengue virus in larval forms of aedes aegypti and to associate vector presence with rainfall and incidence of disease.200819031506
granulomatous myocarditis and coelomic effusion due to salmonella enterica arizonae in a madagascar dumerili's boa (acrantophis dumerili, jan. 1860).a granulomatous myocarditis due to salmonella enterica arizonae was diagnosed in an 8-year-old madagascar dumerili's boa (acrantophis dumerili) based on positive coelomic effusion culture, ultrasound visualization of abnormal ventricular myocardium, necropsy and cardiac histological examination.200319081357
molecular characterisation of cryptosporidium isolates from pet reptiles.in this study, we investigated the presence of cryptosporidium in 171 faecal samples from reptiles commonly used as pet animals. these include lizards belonging to the genera eublepharis, pogona, chlamydosaurus, hemiteconyx, teratoscincus, tiliqua, iguana, and chamaeleo, snakes of the genera lampropeltis, elaphe, python, boa and corallus, and tortoises belonging to the genera testudo and kinixys. cryptosporidium oocysts were detected by immunofluorescence using a commercially available kit and c ...200919101086
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