| a controlled study of allergen production in cultures of dermatophadoides pteronyssinus. | allergenic substances have been isolated from cultures of dermatophagoides pteronyssinus on wheat germ flakes and powdered yeast; as controls, non-sterile nutrient medium containing no mites, as well as sterile medium, were maintained and extracted under identical conditions. chemical purification and analyses indicated the occurrence of skin-active allergens in all three preparations. this was confirmed by the assay of skin-reactivity in vivo and complement-inactivating capacity in vitro. quant ... | 1975 | 53984 |
| electron-microscopic study of the anaphylactic degranulation of human basophils from atopic subjects. | leukocytes from subjects allergic to dermatophagoides pteronyssinus were incubated for 20 min with a solution of d. pteronyssinus extracts. histamine release was measured at 0, 3, 10 and 20 min. simultaneously, samples were treated for electron microscopy in such a way as to correlate histamine release and the morphological aspects of basophil leukocytes. the principal features accompanying histamine release were: a progressive activation of the cytoplasmic membrane which showed long processes, ... | 1978 | 78905 |
| [immunologic investigations of house-dust and house-dust mites. i. schultz-dale test (author's transl)]. | with dermatophagoides pteronyssinus and house-dust sensitized guinea-pigs showed cross-reactions between house-dust, d. pteronyssinus and d. farinae in the schultz-dale test. euroglyphus maynei gave positive reactions only in animals sensitized with d. pteronyssinus. the stored food mites tyrophagus putrescentiae, chortoglyphus arcuatus and glycyphagus destructor and the rearing medium (horse dander/yeast) did not cause any cross-reactions in any test group. | 1977 | 145169 |
| standardization of dermatophagoides pteronyssinus extracts and tyrosine adsorbates. | human skin test, inhibition of rast and the radioimmunosorbent technique of ceska have been shown to be useful assays for the standardization of d. pteronyssinus aqueous extracts. the development of these assay systems for the standardization of d. pteronyssinus tyrosine adsorbed formulations is described. tyrosine solubilisation procedures are detailed together with the influence of these procedures on the three assays. good correlation has been shown between the human skin test and the radioim ... | 1975 | 238873 |
| [allergy to house dust in patients with asthma. iii. ige level and specific ige antibodies against house dust allergens, dermatophagoides pteronyssinus, aspergillus fumigatus and cladosporium herbarum in the serum of patients with asthma]. | | 1977 | 319433 |
| a clinical environmental study of the aeroallergens of the islands of bermuda. | the major allergen responsible for allergic respiratory disease in the islands of bermuda has been shown to be derived from dermatophagoides pteronyssinus. 73% of the atopic group included in the survey gave weal and erythema reactions to extracts of this mite, whereas only 30% reacted to mixed pollen extracts and 10% to mould extracts. d. pteronyssinus was isolated from all house dust samples and it represented nature, with only a modest seasonal influence, and pollen counts were low throughout ... | 1978 | 361282 |
| mites in house dust in the stockholm area. | today there is no doubt that mites (and especially species within the pyroglyphidae family: dermatophagoides pteronyssinus and dermatophagoides farinae) are an important agent in dust allergies. the author reports on a study of the occurrence of mites in house dust in the environment of allergic persons in the stockholm area. 201 private homes, 13 farmers' houses and two hospitals have been studied. in 12% of the homes several different types of mite were found in dust in relatively small number ... | 1979 | 378014 |
| [specific and nonspecific hyposensitization of animals with experimental allergy to the antigen from dermatophagoides pteronyssinus mites]. | | 1979 | 424244 |
| studies on the physicochemical properties of house dust mite (dermatophagoides pteronyssinus) allergens involved in reaginic reaction. | the physicochemical nature of the allergenic component of dermatophagoides pteronyssinus extract was studied by gel filtration, ion exchange chromatography, isoelectrofocusing, and polyacrylamide gel electrophoresis. fractions were assayed for allergenic activity by the direct radioallergosorbent test (rast) (dilution method), rast inhibition (50% inhibition method) and human skin testing. fractions were further studied by preparative polyacrylamide gel electrophoresis and the band showing a flo ... | 1979 | 470186 |
| bronchial asthma in nigerian children. | clinical and laboratory studies on bronchial asthma in 200 nigerian children who were seen during a 2 1/2-year period in ibadan are described. contrary to reports that the condition is rare in african children, after pulmonary tuberculosis, asthma is the next most common chronic chest disease in ibadan. while many features of the disease are similar to those seen elsewhere, skin tests indicate that ascaris is the most common antigen associated with asthma, followed by dermatophagoides pteronyssi ... | 1979 | 475428 |
| inhibition experiments with solid phase mite or epithelia in house dust hypersensitivity. | the present study was undertaken to verify that mites are not the only allergens in house dust extracts and that other allergens such as cat epithelia can also be responsible for house dust hypersensitivities detected both by house dust skin tests and house dust rast studies. in order to determine whether mite or epithelia fixed on a solid phase could remove not only the ige antibodies reactive with the homologous allergens, but also the ige antibodies reactive with house dust allergens, the aut ... | 1979 | 546247 |
| house dust mites in switzerland. iii. allergenic properties of the mites. | the allergenic properties of the nine mite species prevalent in the house dust of northwestern switzerland (basel and surroundings) were studies. for the preparation of mite extracts, the average weight of each species was first determined and then the number per culture glass counted. thus the extracts were prepared according to the weight of the mites. they were tested on 115 patients with ashma and/or rhinitis. tests with patients own house dust and with a human dander/yeast mixture, which s ... | 1977 | 579046 |
| selective isolation of allergens. i. reaction of house dust mite extracts with tridacnin and concanavalin a and examination of the allergenicity of the isolated components. | tridacnin, a lectin from the clam tridacna maxima was found to precipitate with crude extracts from the house dust mites dermatophagoides farinae and dermatophagoides pteronyssinus. using tridacnin in direct precipitation and affinity chromatography studies, a carbohydrate-rich preparation of high molecular weight was isolated from d. farinae extracts. the isolated preparation gave one precipitin line when used with tridacnin in gel diffusion and immunoelectrophoresis experiments but two bands w ... | 1977 | 589778 |
| production of guinea pig reaginic antibody against the house dust mite extract, dermatophagoides pteronyssinus, without adjuvant. | guinea pigs of hartley strain were sensitized with an extract of the house dust mite, dermatophagoides pteronyssinus. positive immediate skin reactions were obtained in more than half of the animals 6 weeks after repeated intradermal injection. adjuvants of a1(oh)3 gel or bordetella pertussis vaccine did not significantly enhance the production of reaginic antibody. prausnitz-küstner type skin test titers were even higher in guinea pigs sensitized with the mite extract without adjuvant. circadia ... | 1977 | 599718 |
| [dermatophagoides pteronyssinus and dermatophagoides farinae from house dust in columbia]. | | 1977 | 605992 |
| [significance of the radioallergosorbent test (rast) in the specific diagnosis of atopic bronchial asthma (author's transl)]. | the radioallergosorbent test (rast) is a recently developed radioimmunological in-vitro method for determining ige-specific serum antibodies in reaginic allergy. there was 63.4% overall correspondence between rast and bronchial provocation test in 423 tests with a total of eight perennial allergens on adults with asthma. where there was disagreement, combination of postiive inhalation test and a negative rast was much more frequent (33.6%) than in the obverse (3%). agreement between rast and pro ... | 1978 | 639694 |
| tyrophagus putrescentiae: an allergenically important mite. | the incidence of positive skin tests to the mite tyrophagus putrescentiae was measured and compared with skin reactions to dermatophagoides pteronyssinus and other allergens in australian and new guinean populations, and mite counts were determined in sydney and new guinea houses. positive reactions to t. putrescentiae were as frequent as those for d. pteronyssinus in asthmatics in sydney and were the commonest positive reactions in the normal new guinea population. t. putrescentiae-specific ser ... | 1978 | 647897 |
| [antibody formation in experimental tick (dermatophagoides pteronyssinus) allergy]. | | 1978 | 651822 |
| [experimental aerosol sensitization with an antigen from dermatophagoides pteronyssinus ticks]. | | 1978 | 704172 |
| [ecological study of pyroglyphides mites in house dust in the grenoble area. a qualitative approach and incidence of various parameters: seasons, altitude, temperature and relative dampness in their multiplication]. | this study concerns the analysis of 70 samples of dust mites taken periodically from 13 sample areas or mattresses at different altitudes. it shows the presence of pyroglyphid mites in all the mattresses studied. four species were found: dermatophagoides pteronyssinus, dermatophagoides farinae, euroglyphus maynei and dermatophagoides evansi. from a quantitative point of view, the statistical analysis of the results obtained allows the following conclusions to be drawn: -- all the pyroglyphids (a ... | 1978 | 754622 |
| sudden infant death syndrome in south australia. measurement of serum ige antibodies to three common allergens. | radioallergosorbent test (rast) studies showed that ige antibodies to dermatophagoides pteronyssinus (house dust mite), aspergillus fumigatus and bovine beta-lactoglobulin were significantly elevated in the sera of infants who died as a result of the sudden death in infancy syndrome (sdis). no significant differences were found in the levels of total ige, iga, igg or igm in the sera of sdis victims or controls. the possible role of hypersensitivity in the aetiology of sdis is discussed. | 1975 | 768723 |
| antigenic and allergenic constituents of dermatophagoides pteronyssinus extract. | the antigenic analysis of dermatophagoides pteronyssinus extract was performed by using antiserum obtained in rabbits by immunization with mite extract in freund's adjuvant. immunodiffusion and immunoelectrophoretic analysis revealed the presence of many constituents, most of which were common to human dander, house dust and dermatophagoides farinae extracts. by fractionation of the mite extract on sephadex g-75 and deae-sephadex it was shown that the highest allergenic activity was related to a ... | 1975 | 807491 |
| [adrenergic and cholinergic structures of guinea pig lungs under normal conditions and in experimental bronchial asthma]. | fluorescent and histochemical methods were applied to the study of the morpho-functional condition of the adrenergic and cholinergic nervous system of the bronchopumonary apparatus in guinea pigs under normal conditions and during sensitization (subcutaneous and inhalant) with tick dermatophagoides pteronyssinus ticks. there was revealed a marked excitation of the adrenergic nervous system, and a reduction of the acetylcholine esterase activity in the bronchopulmonary tissue during the animal se ... | 1977 | 857971 |
| domestic acari of colombia: abundance of the european house dust mite, dermatophagoides pteronyssinus (acari: pyroglyphidae), in homes in bogatá. | | 1977 | 886563 |
| [experimental sensitization with an extract of dermatophagoides pteronyssinus ticks]. | immunogenic properties of dermatophagoides pteronyssinus ticks were studied in subcutaneous injection of the antigen to guinea pigs and rabbits. marked changes in the immunological reactivity of the animal organism were revealed; these changes were accompanied by production of agglutinating, sensitizing the mast cells and precipitating (in rabbits) antibodies, and by the development of hypersensitivity of the immediate and delayed type. | 1976 | 1026303 |
| clinical significance of the house dust mite (dermatophagoides pteronyssinus) in asthmatic children in japan. | clinical studies were conducted to evaluate the role of the house dust mite. dermatophagoides pteronyssinus, as an allergen in children's asthma in japan. skin testing and prausnitz-küstner type skin testing showed good correlations between the allergenicity of house dust and the mite. a positive prausnitz-küstner type skin reaction to house dust was neutralized by a mite extract. radioallergosorbent test showed good correlation between house dust and the mite (r=0.687). the mite exhibited in vi ... | 1976 | 1037059 |
| mites and house dust allergy. iv. antigen and allergen(s) of dermatophagoides pteronyssinus extract. | the antigenic composition of d. pteronyssinus extract was investigated by using antisera raised in rabbits by injection of the mite extract in freund's adjuvant. immunodiffusion analysis revealed the presence in the mite extract of many antigenic constituents, most of which were also common to human dander and house dust extracts. four antigenic components not shared by human dander appeared to be common to d. farinae extract. d. pteronyssinus extract was then fractionated on sephadex g-75 and d ... | 1975 | 1053431 |
| ige antibodies to dermatophagoides pteronyssinus (housedust mite), aspergillus fumigatus, and beta-lactoglobulin in sudden infant death syndrome. | the prevalence of serum ige antibodies to dermatophagoides pteronyssinus (house-dust mite), aspergillus fumigatus, and bovine beta-lactoglobulin was significantly greater in cases of sudden infancy death (s.i.d.) than in a control group of infants of the same age range. this difference was most pronounced with d. pteronyssinus antibodies, which suggests that hypersensitivity to house-dust mite may be a factor in the aetiology of s.i.d. in western australia. both the s.i.d. and control infants ha ... | 1975 | 1090327 |
| reaginic bronchial allergy and the occurrence of reagins against mites in a representative sample of a local swedish population. | this study involves an investigation of 185 subjects, aged 35--54 years, a representative sample of persons with chronic obstructive lung disease in a local swedish population. bronchial asthma was diagnosed in 86 persons, chronic bronchitis in 69 and a combination of bronchial asthma and chronic bronchitis in 30. reaginic bronchial allergy as a provoking factor was considered as definite when the case history, an intracutaneous test, a bronchial provocation test and a radioallergosorbent test w ... | 1975 | 1145150 |
| asthma in the highlands of new guinea total ige levels and incidence of ige antibodies to house dust mite and ascaris lumbricoides. | serum ige levels in adults from the highland area of papua-new guinea were considerably elevated above those found for caucasian residents in australia and most western countries. the median ige value for the asthmatic group in new guinea was significantly (p less than 0.01) elevated above that of control subjects. identification of ige antibodies by the radio-allergosorbent test to house dust mite showed an 85-percent correlation with the results of skin reactions to extracts of the mite. 90% o ... | 1975 | 1170138 |
| population structure and dynamics of the house dust mite dermatophagoides farinae (acarina: pyroglyphidae) in czechoslovakia. | the relationship between the room microclimate and population dynamics of the house dust mite dermatophagoides farinae hughes, 1961 was studied. a distinct increase of the mite population density in mattresses was observed only in the period, when the monthly means of relative air humidity in rooms were higher than 47-50%, denominated by the author as critical air humidity. this is a considerably lower value than that relevant to dermatophagoides pteronyssinus (trouessart, 1897) from the paper b ... | 1975 | 1193484 |
| [acarofauna of house dust in barcelona and surrounding cities]. | the study of the house-dust acarofauna has aroused great interest since voorhorst and colls. (41), in 1964, showed the continual presence of dermatophagoides pteronyssinus in this habitat; and considered by these authors to be mainly responsible for the allergenic capacity of housedust. studies carried out by other authors around the world confirm the universal character of this acarus and its prevailing presence in house-dust biocenosis. dermatophagoides pteronyssinus is usually accompanied wit ... | 1975 | 1221825 |
| [influence of acari of house dust in the tiology of infantile asthma]. | we have studied 61 children with a previous diagnosis of a possible allergic disease showing clear symptoms in relation to the respiratory system (rhinitis, asthma, etc.). in all cases prick tests were carried out with house-dust extracts dermatophagoides pteronyssinus, d. farinae, t. putrescentiae, a. siro and a. siro + cheyletus spp. commercial extracts (bencard) were used for the former two; the remainder were prepared from the pure culture of the corresponding acari. the technique followed h ... | 1975 | 1221826 |
| [determination of specific ige serum antibodies using the radio-allergo-sorbent-test (rast) and its significance for the diagnosis of atopic allergy]. | the radio allergo sorbent test (phadebas-rast) is a recently developed radioimmunological in-vitro test for determining ige specific serum antibodies in reaginic allergy. the overall correspondence between rast and skin tests in 1,128 tests with a total of 19 allergens was 70.2%. the agreement between positive rast and skin tests was 22.1%. in instances of disagreement the combination of a positive skin test and a negative rast was much more frequent (24.1%) than the reverse (5,7%). the agreemen ... | 1975 | 1226511 |
| a successful method for mass culture of the house dust mite, dermatophagoides pteronyssinus (troussart, 1897). | comparative studies have been carried out to find out an optimum condition for efficient mass culture of dermatophagoides pteronyssinus (troussart, 1897), the most dominant species of the house dust mites. among various food materials tested for culture media, the mixture composed of 2 parts of powdered laboratory animal food, 2 parts of dried yeast and 1 part of dried fish powder was found to be most fitted. the humidity content of the medium was best when adjusted to 16% beforehand and thin la ... | 1975 | 1228234 |
| ige antibodies to house dust, mite, animal allergens and moulds in house dust hypersensitivity. | fifty-three patients were investigated by intradermal tests and phadebas rast and, in most cases, the serum ige level was determined. they were tested for sensitivity to house dust, dermatophagoides pteronyssinus, cat, dog, horse and mould allergens. all cases with ige antibodies to house dust were found to have specific ige to other tested allergens as well. eighty-nine per cent of the patients being rast positive for house dust were also positive for d. pteronyssinus and in the majority of tho ... | 1976 | 1277438 |
| analysis of antigenic determinants shared by two different allergens recognized by human t cells: house dust mite (dermatophagoides pteronyssinus) and chironomid midge (chironomus yoshimatsui). | to analyse the cross-reactivity of t-cell-mediated immunity between dermatophagoides pteronyssinus (dp) and chironomus yoshimatsui (cy), the most common allergens in japan, we established antigen-specific human t-cell lines and clones. some but not all of the cy-induced t-cell lines showed a significant proliferative response not only to cy, but also to dp. no t-cell line responded to other unrelated antigens. when we stimulated the dp-induced t-cell clones with cy, 3 of the 40 clones (7.5%) sho ... | 1992 | 1283062 |
| allergic skin reactivity in 232 patients over 50 years. | we examined two hundred and thirty-two out-patients, ranging in age from 50 to 75 yrs. all patients were submitted to skin-tests: prick and, if it was necessary, intradermal reactions and/or rast. allergens employed were gramineae, parietaria, olea, dermatophagoides farinae, dermatophagoides pteronyssinus, house dust, cat and dog skin scales. one hundred and twenty-eight subjects had positive responses to one or more aeroallergens. asthma was the most frequent condition both in skin-positives an ... | 1992 | 1292328 |
| plasma kallikrein amidolytic activity in bronchial asthma. | we have investigated plasma kallikrein amidolytic activity in the following groups of patients: 1. normal control group of blood donors. 2. extrinsic pollen-activated bronchial asthma patients, during periods of symptomatology and at a later time after the spring. 3. subjects with atopic bronchial asthma in acute phase when admitted to our hospital's emergency room and later when clinically recovered. 4. subjects with extrinsic bronchial asthma, sensitive to dermatophagoides pteronyssinus and de ... | 1992 | 1292330 |
| skin sensitivity patterns to inhalant allergens in nigerian asthmatic patients. | the pattern of skin sensitivity response to inhalant allergens in 746 nigerian asthmatics and 92 normal controls tested over 10 years period is reported. significant differences in reactions were observed in 7 of the 16 allergens routinely used in the study. house dust mite--dermatophagoides pteronyssinus gave the highest incidence of positive skin reaction, 58% asthmatics, 4.3% controls (p < 0.001). other significant allergens were house dust 51.7%, feathers 24.4%; dog hair 12.9%, cat fur 11.9% ... | 1992 | 1298621 |
| biological characterization of a formulated allergoid from dermatophagoides pteronyssinus. | a comparative study between the biological properties of the allergen dermatophagoides pteronyssinus (dp) and its formulated allergoid (dphcho) was performed. after immunizing rats with dp or dphcho the serum level time course of ige and igg was determined by passive cutaneous anaphylaxis (pca) and indirect-elisa, respectively. the avidity for ige (pca and pca-inhibition) or igg (indirect elisa and elisa inhibition) and the ability to induce anaphylactic shock were also determined. serum ige pat ... | 1992 | 1302894 |
| t cell activation-inducing epitopes of the house dust mite allergen der p i. proliferation and lymphokine production patterns by der p i-specific cd4+ t cell clones. | cloned human cd4+ t cell lines specific for the house dust mite dermatophagoides pteronyssinus were used to map minimal t cell activation-inducing epitopes on the group i allergen in d. pteronyssinus extracts (der p i) molecule. most of these der p i-specific t cell clones expressed different tcr v alpha and v beta gene products. using recombinant deletion proteins, three t cell epitopes were identified on the der p i molecule; p45-67 and p117-143 were recognized by hla-dr7-restricted t cells, w ... | 1992 | 1370514 |
| house dust mite-specific t cells in the skin of subjects with atopic dermatitis: frequency and lymphokine profile in the allergen patch test. | the mechanism underlying positive patch tests with house dust mite-allergen, dermatophagoides pteronyssinus (der p), in patients with atopic dermatitis was investigated by isolating t cells from the test sites of two patients. eighty-five t cell clones (tcc) were established from the epidermis and dermis of lesional skin by the limiting-dilution method with der p and interleukin (il)-2. with restimulation assays, 29 of 60 tccs tested demonstrated specific proliferation; 85% were of the cd3+, cd2 ... | 1992 | 1373161 |
| localization of allergens in the domestic mite lepidoglyphus destructor. | the non-pyroglyphid domestic mite lepidoglyphus destructor is a major source of allergen causing respiratory symptoms in farming environments. this study is the first to focus on the localization of the allergens in the non-pyroglyphid domestic mite lepidoglyphus destructor. cryostat-cut sections of l. destructor mite bodies and faecal pellets were probed with one of three mouse monoclonal antibodies (moabs) raised against l. destructor or with patient sera, and stained with immunoperoxidase. ei ... | 1992 | 1373986 |
| specific histamine release capacity of peptides selected from the modelized der p i protein, a major allergen of dermatophagoides pteronyssinus. | dust mite allergens are considered as a major cause of allergic disease and as a risk factor for asthma. der p i, a 222 amino-acid residue globular glycoprotein, is one of the major allergens from dermatophagoides pteronyssinus (dpt) mites. in this study, we have used predictive conventional algorithms (i.e. hydrophilicity, mobility, accessibility) and a three-dimensional model of der p i derived from comparison to actinidin and papain to select continuous amino acid sequences as potential b cel ... | 1992 | 1376413 |
| peptide-induced nonresponsiveness of hla-dp restricted human t cells reactive with dermatophagoides spp. (house dust mite). | the activation of cd4+ t lymphocytes, which play a central role in allergic inflammation, depends on the recognition of allergen-derived peptides in association with major histocompatibility complex class ii gene products. in this report we demonstrate, at a clonal level, that a component of the t-cell repertoire reactive with dermatophagoides spp. (house dust mite) in atopic individuals, is restricted by hla-dp class ii molecules. this supports the recent results emerging from genetic epidemiol ... | 1992 | 1385507 |
| specific activation of platelets from patients allergic to dermatophagoides pteronyssinus by synthetic peptides derived from the allergen der p i. | previous studies have shown that fc epsilon rii-bearing platelets from patients with allergic disorders could be stimulated either by anti-human ige antibodies or the relevant allergens to generate cytotoxic mediators. in this report, the reactivity of platelets from dermatophagoides pteronyssinus (d. pt)-sensitive patients to three der p i-derived synthetic peptides (52-71, 117-133 and 188-199) was investigated. the platelet stimulation observed in d. pt-sensitive patients was demonstrated to b ... | 1992 | 1386546 |
| immunopathology of sids. | immunopathological studies of sids share the problems of all necropsy based studies of this syndrome: the extent of autolytic changes in the material under study; and the lack of appropriate controls. despite these problems, several studies have been performed on serum, bronchoalveolar lavage, and pulmonary tissue. many of these studies have been inspired by the modified anaphylaxis hypothesis, based on the experiments of coombs and coworkers. lightly anaesthetised guinea-pigs, which had been se ... | 1992 | 1474158 |
| how relevant are house dust mite-fungal interactions in laboratory culture to the natural dust system? | both house dust and house dust mites dermatophagoides pteronyssinus contained a wider range of fungi than laboratory mite cultures. in total, nine species of fungi were isolated from d. pteronyssinus in house dust, and these included three xerophilic species (eurotium amstelodami, aspergillus penicillioides and wallemia sebi) commonly found in laboratory cultures of d. pteronyssinus. it is concluded that mites do interact with a similar range of fungi in natural dust and in laboratory culture, b ... | 1992 | 1493747 |
| [bronchial asthma in children with sensitization to mites]. | overall 180 children with bronchial asthma induced by sensitization to dermatophagoides pteronyssinus and dermatophagoides farinae were examined clinically and immunologically. the patients' age ranged from 1 to 14 years. a relationship was established between bronchial asthma exacerbation and the stay at home. in that disease pattern, asphyxia attacks were noted to occur more frequently in the evening and night hours. emphasis is laid on the leading part played by the ige-mediated mechanism in ... | 1992 | 1513646 |
| the house dust mite dermatophagoides pteronyssinus is the most important allergen on the island of mauritius. | to determine the relative importance of mites as a cause of allergic sensitivity and asthma on the western indian ocean island of mauritius, we measured specific ige antibodies to common inhalant allergens in sera from mauritians claiming to have allergic symptoms and we examined house dust samples for evidence of mites and their allergens. seventy-two of the 110 sera tested (65%) contained detectable ige antibody to at least one mite, mould or pollen allergenic extract. by far the most prevalen ... | 1992 | 1628251 |
| reduction of environmental mites improved atopic dermatitis patients with positive mite-patch tests. | during a series of studies on the involvement of house dust mite antigens in 183 cases of atopic dermatitis, we observed an improvement in two patients following the removal of mites from their environment by means of a thorough housecleaning and replacement of the mattress. both patients manifested the typical clinical skin lesions of atopic dermatitis and had similar laboratory findings. although the serum ige concentrations and specific ige to dermatophagoides pteronyssinus and dermatophagoid ... | 1992 | 1640023 |
| skin cross-reactivity between dermatophagoides pteronyssinus and storage mites in atopic children. | in our environment, some children with allergic respiratory pathology do not have as favorable an evolution as expected after three years of immunotherapy with d. pteronyssinus extracts. we believe this is due to the existence of other mites whose antigenic determinants are not present in d. pteronyssinus. the purpose of the present study was to evaluate skin cross-reactivity between dermatophagoides and storage mites in children with bronchial asthma. fifty patients were chosen, with a mean age ... | 1991 | 1669575 |
| estimation of ige antibodies to the common allergens by reverse enzyme immunoassay. comparison with the radioallergosorbent test. | a reverse enzyme immunoassay (reina) is described, in which polystyrene microtiter wells are sensitized with murine monoclonal anti-human ige, and then sequentially allowed to react with patient's serum, peroxidase-labeled allergens and substrate. the results obtained with the sera of patients allergic to lolium perenne grass pollen, the tree pollens of betula alba and olea europea, the epithelia of cat and dog, the mite dermatophagoides pteronyssinus, or to the foodstuffs cow's milk, chicken eg ... | 1991 | 1669584 |
| increased numbers of th2-like cd4+ t cells in target organs and in the allergen-specific repertoire of allergic patients. possible role of il-4 produced by non-t cells. | phytohemagglutinin (pha)-induced human t cell clones (tcc) derived from conjunctival flogistic tissues of 3 patients with vernal conjunctivitis produced unusually high amounts of interleukin-4 (il-4) and no, or limited amounts of, gamma-interferon (ifn-gamma). allergen (dermatophagoides pteronyssinus or lolium perenne group i)-specific tcc derived from peripheral blood of two atopic donors produced significantly higher amounts of il-4 and significantly lower amounts of ifn-gamma than tcc specifi ... | 1991 | 1682270 |
| prevalence of specific ige to storage mites (a. siro, l. destructor and t. longior) in an urban population and crossreactivity with the house dust mite (d. pteronyssinus). | the role of pyroglyphid mites in house dust allergy is well established and the major allergens from the common house dust mites (dermatophagoides species) have been characterized. there is, however, relatively little progress in the understanding of the human ige response to non-pyroglyphid storage mites, allergenic crossreactivity with other mite species and extent of environmental exposure. we studied 196 individuals from an urban environment who were not occupationally exposed to storage mit ... | 1990 | 1695869 |
| the characteristics of antigen define the cellular source and kinetics of histamine-releasing factor induced by human peripheral-blood mononuclear cells. | peripheral-blood leucocytes from dermatophagoides pteronyssinus- and/or lolium perenne-allergic patients produce in vitro histamine-releasing factor (hrf) in an allergen-specific and concentration-dependent relationship. maximum production of hrf occurred in cultures containing as little as 10-100 pg/ml crude allergen extract, although a second peak occurred at higher concentrations (10-100 micrograms/ml). while hrf was detectable in 1-hour cultures, maximal production required 24 h in culture. ... | 1991 | 1718885 |
| epitope mapping of the dermatophagoides pteronyssinus house dust mite major allergen der p ii using overlapping synthetic peptides. | fourteen synthetic peptides of 15 amino acid residues length, overlapping by five residues and spanning the entire sequence of the major allergen der p ii from the house dust mite dermatophagoides pteronyssinus were synthesized. these peptides were coupled to cnbr-activated sepharose-4b and used as solid-phase antigens in epitope mapping studies using human ige antisera. these antibodies bound predominantly to the peptide comprising residues 65-78, the binding of which was inhibited by native de ... | 1991 | 1720504 |
| mucosal immunity in primary glomerulonephritis: ii. study of the serum iga subclass repertoire to food and airborne antigens. | iga specific for 7 food and 6 airborne antigens were sought in the serum of 30 adult patients with iga mesangial nephropathy (iga gn), 23 with membranous nephropathy (mgn), 20 with idiopathic nephrotic syndrome (ins), 11 with membranoproliferative gn (mpgn) and 22 healthy controls by means of an enzyme-linked immunoassay. the iga subclass was determined using monoclonal antibodies. increased levels of iga specific for gliadin, bovine serum albumin (bsa), ovalbumin, lysozyme and alpha-lactalbumin ... | 1991 | 1766494 |
| asthma in tanta, egypt: serologic analysis of total and specific ige antibody levels and their relationship to parasite infection. | the relationship between asthma, ige and parasite infection was compared in 68 randomly selected patients with asthma and 37 nonasthmatic controls living in tanta, egypt. sera were assayed for total ige and for ige antibodies to inhaled allergens (mite, cat, cockroach, ryegrass, ragweed and 3 fungi) and to parasite antigens (schistosoma mansoni and brugia malayi). parasite infection was determined by microscopic examination of stool specimens. total ige levels were significantly higher in patien ... | 1991 | 1809691 |
| analysis of house dust mite-specific ige, igg4, and igg antibodies during immunotherapy in asthmatic children. | house dust mite (dermatophagoides pteronyssinus, dp) specific ige, igg4, and igg antibodies were analyzed by western blotting in 18 asthmatic children during the course of 2-years' allergen-specific immunotherapy. although more than 50 protein bands could be identified in the crude mite extracts by sds-page, only eight of them elicited both ige and igg4 antibodies in most patients. the molecular weights were 110, 60, 55, 43, 33, 27, 16, and 14 kd, respectively. no antibodies against new antigens ... | 1991 | 1859043 |
| identification of major allergens from the house dust mites, dermatophagoides farinae and dermatophagoides pteronyssinus, by electroblotting. | the allergens were separated from the extracts of house dust mites by sds-polyacrylamide gel electrophoresis (sds-page) and identified by autoradiography. over 30 protein bands of the whole body extract of dermatophagoides farinae were apparent on 10-20% gradient sds-page, and 13 bands with mw between 93kd and 12kd bound with specific ige antibodies in patients' sera sensitive to house dust mites. the major allergenic component of the whole body extract of d. farinae was the protein of mw 14-15k ... | 1991 | 1877252 |
| diagnosis of nasal allergy to the house dust mite. | twenty-five patients with perennial rhinitis and a positive skin prick test (spt) for dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) were submitted to nasal provocation and the radioimmunosorbent test (rast) for specific ige-antibodies. we found a significant correlation in the reaction to both allergen extracts for all parameters examined. in addition, there was a significant correlation among the spt, the rast and the nasal provocation for dp and between the spt and the ... | 1991 | 1891679 |
| exposure and sensitization to dust mite allergens among asthmatic children in são paulo, brazil. | a group of 20 mite allergic asthmatic children aged 6-12 years old, living in são paulo, brazil, was studied regarding their degree of sensitization to house dust mites and exposure to mite allergens in their homes. in 18 out of 20 houses at least one dust sample was obtained which contained greater than 10 micrograms der p i/g of dust. the highest levels of dermatophagoides pteronyssinus allergens, der p i and group ii, were measured in bedding samples (geometric mean 38.4 and 36.6 micrograms/g ... | 1991 | 1913266 |
| [immunologic response to microorganisms from a polluted ventilation system in a working environment]. | authors point out the morbidity of employees working in ventilation systems contaminated with various microorganisms. they analysed 96 workers exposed to air conditioning system (group a), and 71 workers (group b) breathing normal ambient air. the workers of both groups were subjected clinically by functionally and immunologically. preparation of antigens "mmm" (monday morning miseries) was used as an original method by ajello et al. for producing antigens from systemic mycotic agents and subseq ... | 1991 | 1916435 |
| [the significance of individual inhalation allergens in the sensitization of children with respiratory allergy]. | four hundred children were skin tested with following allergens: dermatophagoides pteronyssinus, moulds, animal dander (mixture of dog, cat, sheep and horse dander), tree pollens, weed pollen (ambrosia artemisifolia), and six grass pollens, as well as three nutritive allergens in children less than three years of age. results demonstrated that in 200 children with perenial rhinitis and/or asthma. dermatophagoides gave positive results in over 90%. in 200 children with pollen allergy, grasses wer ... | 1991 | 1916444 |
| nutritional value of yeast for dermatophagoides pteronyssinus (acari: epidermoptidae) and the antigenic and allergenic composition of extracts during extended culturing. | cultures of european house dust mite, dermatophagoides pteronyssinus (trouessart), were evaluated with respect to yeast nutritional value, together with an assessment of the antigenic and allergenic composition. cie analysis of extracts illustrated that yeast contributes many antigens throughout the rearing period. however, there is an indication of the consumption of only one particular yeast antigen by the mites. total protein content profile of the mite cultures is shown in addition to the to ... | 1991 | 1941906 |
| diagnostic performance characteristics of the standard phadebas rast, modified rast, and pharmacia cap system versus skin testing. | results from three in vitro assays for allergen-specific ige, the standard phadebas radioallergosorbent test (phrast), modified rast (mrast), and the new pharmacia cap system (cap) were compared with skin prick testing (spt) results in 104 patients with allergic rhinitis and/or asthma and 24 nonatopic controls. five allergens were evaluated: cat, dermatophagoides pteronyssinus, alternaria, june grass, and short ragweed. using spt results as the reference standard, the phrast had the lowest sensi ... | 1991 | 1958005 |
| preparation of specific monoclonal antibodies against der f ii one of the major allergens of dermatophagoides farinae. | five monoclonal antibodies (mabs) against der f ii, one of the major allergens of dermatophagoides farinae, were obtained from mice immunized with the allergen. western blotting analysis revealed that only one mab, named 15e11, was specific for der f ii, whereas the other mabs crossreacted with der p ii, one of the major allergens of dermatophagoides pteronyssinus. these results indicate the possibility that the der f ii-specific mab, 15e11, is a useful reagent for specific determination of der ... | 1991 | 1958138 |
| caffeine, a naturally occurring acaricide. | since caffeine is a plant alkaloid that has been described as a naturally occurring insecticide, its acaricidal effect on dermatophagoides pteronyssinus (dp) was investigated. twelve cultures were established by adding 30 dp to 200 mg of tetramin fish food and brewer's yeast (8:2 ratio); six cultures were treated with 20 mg of finely ground caffeine. all 12 cultures were incubated at 75% relative humidity, 25 degrees c, and observed during 8 weeks. live mites were then counted under a stereoscop ... | 1991 | 1991914 |
| allergen- and bacterial antigen-specific t-cell clones established from atopic donors show a different profile of cytokine production. | we have established a large panel of t-cell clones (tccs) specific for dermatophagoides pteronyssinus and lolium perenne group i grass pollen allergens (total, 61) and for tetanus toxoid and protein purified derivative bacterial antigens (total, 38) from the peripheral blood of two atopic individuals and then analyzed their ability to produce interleukin 4 (il-4), il-5, and interferon gamma (ifn-gamma). upon stimulation with phorbol 12-myristate 13-acetate plus anti-cd3 antibody, the great major ... | 1991 | 1827920 |
| detection of b-lymphocytes secreting antibodies to dermatophagoides antigens. | an enzyme-linked immunospot assay (eli-spot assay) has been established to count individual cells secreting antibodies to dermatophagoides spp. allergens. initial optimization of the assay was performed using der p i-specific murine hybridoma cell lines. inhibition with soluble purified allergen demonstrated that spot formation was specific. addition of cycloheximide showed that spot formation was due to antibodies actively produced and secreted and not caused by preformed surface-associated ig. ... | 1991 | 2021882 |
| house dust acarofauna and der p i equivalent in australia: the relative importance of dermatophagoides pteronyssinus and euroglyphus maynei. | the acarofauna and der p i allergen concentrations in dust samples from mattresses and lounge room carpets obtained from 20 homes from two coastal cities. perth and bunbury, were determined. all samples were shown to contain mites and the geometric mean numbers of total mites/g of mattress and carpet dust for perth and bunbury were 480 and 263, and 585 and 992, respectively. carpets from both centres had a significantly (p less than 0.02) greater mean number of mite species (perth 9.1, bunbury 9 ... | 1991 | 2043990 |
| specific igg and ige responses to dermatophagoides pteronyssinus in sprague-dawley rats. | specific igg and ige antibodies against the mite dermatophagoides pteronyssinus (dp) have been determined in the serum of sprague dawley (sd) rats by enzyme linked immunosorbent assay (elisa) and passive cutaneous anaphylaxis (pca), respectively. immunization of rats with dp produces a rapid increase of serum specific ige antibody levels which decrease when specific igg antibodies start rising. the results indicate that the sd rat is a good model to induce a humoral immune response to allergen d ... | 1990 | 2099531 |
| expression of dermatophagoides pteronyssinus allergen, der p ii, in escherichia coli and the binding studies with human ige. | lambda gt11 clones expressing the major house dust mite allergen, der p ii, have been reported to react with ige in the serum of a high proportion of allergic patients. the clones described, however, only produced small quantities of protein which was not fused to the beta-galactosidase of the vector. a construct of the der p ii is described which produces a fusion of der p ii, minus its leader sequence, with the glutathione-s transferase in the pgex vector. this could be readily isolated and wa ... | 1990 | 2187817 |
| human basophil releasability. vi. changes in basophil releasability in patients with allergic rhinitis or bronchial asthma. | we evaluated basophil releasability in two groups of allergic patients with positive skin tests to dermatophagoides pteronyssinus major allergen (der p l) (29 adults with bronchial asthma and 17 with allergic rhinitis) and in 31 age-matched normal donors. both basophil reactivity (maximal percent histamine release) and basophil sensitivity (the concentration that causes 50% of maximal percent histamine release: hc50) to der p l in patients with asthma were similar to those in patients with aller ... | 1990 | 1700653 |
| comparison of a multi-allergen dipstick ige assay to skin-prick test and rast. | a multi-allergen dipstick enzyme immunoassay 'quidel allergy screen' (qas) has recently been developed commercially for measuring ige antibodies against nine allergens (house dust 1, house dust 2, dermatophagoides pteronyssinus, d. farinae, japanese cedar, ragweed, cat dander, sweet vernal grass, and egg white) at one time. to assess whether this assay is useful in screening allergen-specific ige antibody, we compared the titres of ige antibodies against the nine allergens measured by qas to tho ... | 1990 | 2192782 |
| aberrant t cell regulation of ige production in atopy. | atopy is associated with elevated serum levels of allergen-specific immunoglobulin e (ige). ige production is preferentially induced by the t cell-derived lymphokine interleukin-4 (il-4) and is suppressed by interferon and prostaglandins, of which interferon-gamma (ifn-gamma) is produced by t cells. we review our studies on the question of whether atopy is associated with cd4+ allergen-specific t cells with an unbalanced lymphokine production. we prepared panels of housedust mite (dermatophagoid ... | 1991 | 1683236 |
| human atopen-specific types 1 and 2 t helper cell clones. | eight representative t lymphocyte clones (tlc) randomly selected from previously described panels of cd4+ housedust mite dermatophagoides pteronyssinus (dp)-specific tlc from atopic and nonatopic donors were studied in more detail in a comparative investigation. the tlc from the atopic donors closely resembled murine type 2 th (th2) cells by secreting substantial il-4, il-5, il-6, tnf-alpha, and granulocyte-macrophage (gm)-csf, minimal ifn-gamma, and relatively little il-2. in contrast, the nona ... | 1991 | 1680923 |
| [results of patch test using mite components in atopic dermatitis (ad) patients. 1st report. "as is patch test" using crushed mites]. | as is patch test using crushed live mites and crushed dried dead mites of dermatophagoides pteronyssinus (d.p.), dermatophagoides farinae (d.f.), & tryophagus ptrescentiae (t.p.) was performed on 49 ad patients and 18 control patients suffering from dermatitis other than ad. results: 1) with crushed dried dead mites all patients showed negative reactions but with live mites 11 among 49 ad patients were positive (22.4%); 2) female mites were more positive than males at the rate 8:1; 3) with cases ... | 1990 | 2273575 |
| house dust mite allergy in florida. mite survey in households of mite-sensitive individuals in tampa, florida. | this study evaluated the prevalence of positive house dust mite skin tests in a population of atopic individuals and identified the mite species present in mattress and house dust samples in homes of the tampa bay area. four hundred consecutive individuals were evaluated for respiratory complaints and skin tested with standardized extracts of dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df). two hundred forty individuals (60%) had a positive skin test to the mite extracts. d ... | 1990 | 2292372 |
| the prevalence of allergic disease and ige antibodies to house dust mite in schoolchildren in taiwan. | the prevalence of positive specific ige antibodies to house dust mites (dermatophagoides pteronyssinus; d. farinae) was determined by enzyme-linked immunosorbent assay (elisa) in 5097 (61%) volunteers of 8345 schoolchildren aged between 7 and 14 yr from two government schools. all of them filled out a questionnaire concerning allergic symptoms. among them, 412 (8.1%) children showed a positive reaction to at least one of the two mite allergens, the range varying between 5.6 and 11.2% according t ... | 1990 | 2310980 |
| ipd-1151t: a prototype drug for ige antibody synthesis modulation. | ipd-1151t [(+/-)-[2-[4-(3-ethoxy-2-hydroxypropoxy)phenylcarbamoyl]-ethyl] dimethylsulfonium p-toluenesulfonate] inhibits not only antigen-induced histamine release from mast cells but also ige antibody formation. the present paper describes the inhibitory effect of ipd-1151t on the ige antibody formation. the ige antibody formation in balb/c mice which had been immunized with dinitrophenylated ascaris extract (dnp.as) plus alum was inhibited dose-dependently by ipd-1151t given p.o. the formation ... | 1991 | 1665310 |
| dermatophagoides and storage mites: statistical analysis of rast results. | r.i.a. dosages of specific ige antibodies to dermatophagoides pteronyssinus, dermatophagoides farinae (house dust mites), acarus siro, lepidoglyphus destructor, and tyrophagus putrescentiae (storage mites) in 467 unselected patients were statistically analyzed. a significant noncorrelation between specific ige antibodies to storage mites and those to house dust mites has been found, suggesting that storage mites have their own allergenic specificity. | 1990 | 2321810 |
| localization of antigens and allergens in thin sections of the house dust mite, dermatophagoides pteronyssinus (acari: pyroglyphidae). | cryostat sections of the house dust mite, dermatophagoides pteronyssinus, were probed in fluorescent microscopy studies with rabbit polyclonal and mouse monoclonal antibodies specific for mite allergens including the major allergen, der p i. sections also were probed for allergens with sera from human mite-allergic subjects containing ige antibodies to mite allergens and with lectins. antibody binding was mainly to the gut lining and gut contents of the mite, although some specific labeling also ... | 1990 | 2332879 |
| isolation of cdna coding for the major mite allergen der p ii by ige plaque immunoassay. | a lambda gt11 library made with cdna from the house dust mite dermatophagoides pteronyssinus was screened with human allergic serum by ige plaque radioimmunoassay. this resulted in the isolation of clones coding for the major allergen der p ii. the cdna coded for a 129-residue protein of 14,131 daltons with no n-glycosylation sites. no sequence homology with other proteins was evident. the der p ii expressed in escherichia coli reacted with ige in 14 of 17 sera from mite-allergic patients giving ... | 1990 | 2341191 |
| immune response to blomia kulagini and dermatophagoides pteronyssinus in sweden and brazil. | the immune responses to the non-pyroglyphid mite blomia kulagini and the pyroglyphid mite dermatophagoides pteronyssinus were compared in 440 swedish farmers and 34 allergic subjects from brazil using the radioallergosorbent test (rast). both b. kulagini and d. pteronyssinus were shown to be a common cause of sensitization, particularly in brazil. the highest rast scores were found against d. pteronyssinus in sera from both countries. allergenic cross-reactivity between the two mites was studied ... | 1990 | 2341199 |
| a study of the relationship between childhood nephrotic syndrome and allergic diseases. | the incidence of atopic diseases in 206 children with nephrotic syndrome (ns) was studied. boys with ns had three times higher incidence of bronchial asthma than the general population. there was no difference in the girls. both boys and girls with ns had about three times more allergic rhinitis and ten times more atopic dermatitis than the general population. in ns patients with associated allergic disease, skin test and allergen-specific ige antibodies by radioallergosorbent test (rast) were p ... | 1990 | 2347227 |
| changes of serum-specific ige antibody titer during hyposensitization in mite-sensitive asthmatic children. | in this study, we used the enzyme-linked immunosorbent assay (elisa) to evaluate the changes of ige antibody titer against dermatophagoides pteronyssinus (d. pteronyssinus) or dermatophagoides farinae (d. farinae) in asthmatic children after immunotherapy. according to elisa analysis, a significantly higher mean level of ige antibody titer to d. pteronyssinus (or d. farinae) was found in nonhyposensitized asthmatic children than in the pediatric control group (p less than 0.001), but there was n ... | 1990 | 2347816 |
| assessment and control of house dust mite infestation. | most allergens of the house dust mite (dermatophagoides pteronyssinus and dermatophagoides farinae) are carried by the mite excreta rather than by the mites themselves. a method of assessing the concentration of mite excreta in dust (the acarex test) is described. the effects of acarosan products, whose active ingredient is solidified benzyl benzoate, on mites and mite excreta were evaluated. over a period of three years, three to four applications of acarosan effectively destroyed mites in carp ... | 1990 | 2379225 |
| functional characterization of lymphocyte response to fractionated house dust mite antigens (dermatophagoides pteronyssinus) in atopic and non-atopic individuals. | this study investigated the cellular response of human lymphocytes to dermatophagoides pteronyssinus crude antigen and 14 molecular weight (mw) fractions. the cells were derived from atopic patients and healthy individuals who were skin test-positive or skin test-negative to mite antigen. when stimulated with crude antigen, the group of patients showed elevated proliferation and production of lymphokine in comparison with the healthy skin test-negative individuals (p less than 0.01). by stimulat ... | 1990 | 2379943 |
| [the histamine liberation test as a diagnostic parameter and its possible modification following specific immunotherapy]. | it is generally accepted that the allergic reaction depends on the levels of specific ige, on the concentration of blocking antibodies and on the capacity of the basophils to release mediators. the fact that in the histamine release test (hrt) all of the elements intervening in an allergic reaction (antigens, ige, basophils and mononuclear cells of a lymphoid origin) have been found, has led some authors to consider it as the most suitable method of "in vitro" diagnosis. this is a reflection of ... | 1985 | 2408457 |
| purification and partial characterization of two major allergens from the house dust mite dermatophagoides pteronyssinus. | two major allergens of the house dust mite, dermatophagoides pteronyssinus (dp), were purified, and their molecular weight and isoelectric points (pis) were determined. dp 42 was purified from an acetone-precipitated mite-excrement extract by a combination of hydrophobic interaction chromatography on phenyl sepharose and copper-chelate chromatography. the molecular weight was determined to be 18,000 and 25,000 to 30,000 by gel filtration (g-75) and sodium dodecyl sulphate-polyacrylamide gel elec ... | 1985 | 2414353 |
| studies on bronchial hyperreactivity, allergic responsiveness, and asthma in rural and urban children of the highlands of papua new guinea. | the prevalence of asthma and allergic responsiveness in rural and urban children of the highlands of papua new guinea was studied. bronchial provocation studies with histamine demonstrated significant bronchial hyperreactivity in 0.5% (1 in 195) rural and 1.7% (1 in 59) urban children, rates which were significantly lower than those observed in corresponding adult populations (7%). urban children demonstrated a higher incidence of skin test reactivity toward dermatophagoides pteronyssinus, asper ... | 1986 | 2420852 |
| cross-reactivity between the house dust mite dermatophagoides pteronyssinus and the mange mites psoroptes cuniculi and p. ovis. i. demonstration of antibodies to the house dust mite allergen dpt 12 in sera from p. cuniculi-infested rabbits. | the cross-reactivity of the mange mites psoroptes cuniculi (pc) and psoroptes ovis (po) antigens with the house dust mite dermatophagoides pteronyssinus (dp) antigens has been studied. cross-reactivity between mange mite and house dust mite antigens was demonstrated by both elisa and immunoelectrophoresis by use of a sheep anti-dp antiserum. both pc and po were demonstrated to contain eight cross-reacting antigens. sera from rabbits infested with pc were demonstrated to produce antibodies to the ... | 1986 | 2426321 |
| antigen der f i from the dust mite dermatophagoides farinae: structural comparison with der p i from dermatophagoides pteronyssinus and epitope specificity of murine igg and human ige antibodies. | the physicochemical and antigenic properties of an allergen purified from dermatophagoides farinae, der f i, were compared with der p i from dermatophagoides pteronyssinus. on sds-page, der f i migrated as a single polypeptide chain with the same m.w. as der p i (24,000). two isoallergenic peaks of der f i were identified on preparative isoelectric focusing (pi 5.7 to 6.3 and pi 6.6 to 6.95). fractions from each peak were shown to have an identical amino acid composition (which was similar but n ... | 1986 | 2428875 |
| comparison by electroblotting of ige-binding components in extracts of house dust mite bodies and spent mite culture. | eight dermatophagoides pteronyssinus extracts (three culture extracts, four mite body extracts, and the world health organization international standard [is]) were investigated by side-by-side sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by electrotransfer to nitrocellulose, and by probing with a pooled serum from mite-allergic subjects. representative body and mite culture extracts were compared by probing with individual sera, and both types of extract were also compared by rast- ... | 1987 | 2433323 |
| investigations of culture medium-free house dust mites. iv. cross antigenicity and allergenicity between the house dust mites, dermatophagoides farinae and d. pteronyssinus. | heterologous crossed immunoelectrophoresis (cie) analysis demonstrated that dermatophagoides pteronyssinus body and d. farinae body extracts contained up to 21 cross-reacting antigens (ags). incubation of cie gels with two mite-sensitive individual sera and a serum pool of mite-sensitive patients indicated that up to nine cross-reacting ags bound mite-specific ige on crossed radioimmunoelectrophoresis and were common allergens. likewise, heterologous cie analysis of d. pteronyssinus feces and d. ... | 1987 | 2434550 |
| selection and characterization of monoclonal antibodies against a major allergen in dermatophagoides pteronyssinus. species-specific and common epitopes in three dermatophagoides species. | hybridomas were produced after immunization with der p i (ag 42, p1). specific anti-der p i activity was detected in a reverse elisa using immobilized anti-mouse ig and highly purified der p i labelled with horseradish peroxidase. stable subclones from 6 parental hybridomas were obtained. the species specificities of the monoclonal antibodies (mabs) were classified: by an overlayer technique analogous to the crie technique, and by two competitive elisa techniques. one mab recognized an epitope s ... | 1987 | 2440816 |
| epitope mapping of two major inhalant allergens, der p i and der f i, from mites of the genus dermatophagoides. | the repertoire of antigenic sites on two major dust mite allergens, der p i of dermatophagoides pteronyssinus and der f i of d. farinae, was studied using murine (balb/c) monoclonal antibodies (mab), polyclonal rabbit igg antibodies, and human ige antibodies. fifty-three igg mab were analyzed from six different fusions (five vs der p i, one vs der f i). by antigen binding radioimmunoassay (ria), most mab were either der p i or der f i specific, and only 2/53 bound to both allergens. epitope mapp ... | 1987 | 2442247 |